Rh1DG381400

SAD/SRA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0002400
Physical Location & Seq
Reverse (-)
60303744 .. 60305856
2113 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG381400.1

Sequence Viewer

Length: 1116 bp
ATGGTTACATTGAAAACACCTGAAAAACAAAATGGGTTTCGTCCCAATTTTAAGAGGCCTAGAGTTTATACAGTTCGAGATTTCCCTGAAGGTTGTGGACGATTTGTGGCTCCAGTGCAGCCAAGTGAATGTGAAGAGGGTGATGAAGATATGAACACAATACCAAGGAAAAATTGCTCCCTGCCTAGTCCTCGAGTTGATGTTGATGTTGTTCGAGATTTTCCTAAAGGTTGTGGGCCGACGGTAGTTTCTTCTTTGAAAGATGCTGAAGAGAAGAGTTTGGTTCCAGACGTTAAAGAATGCGAGCAGCGTGTTGTTGGTCACAAGTTGAGTGTTGCAAGTGTGGTTGATAAAGCTCATAACAAGGCGAAGAAAGCTTTGGCTGCATATCAAGAAAAGAGTTTGGTTCCAGGCGTCAAGGATAAAGAATGCGAGCAGCGTGTTGTTGGTCACAAGTTGAGTGTTGCAAGTGTGGTTGATAAAGCTCATAACAAGGTGAAGAAAGCTTTGGCTGCATATCAAGAGACTTTGATTGAGATGGGTGGAGAATGCGATGCTAATTTCAGCAGACTAAAGGGTTGGAAATTGTACGTTGAAGCAGCAATGTATCTTAAGAAGAAACATAAATGGGTTAACACAAAGAAACAAACAGGGGACATTCCAGGAGTGAAGGTTGGTGACAAGTTTCGGTATAGACCTGAATTAGCTATTGTTGGTCTTCATAGTCACTTTCAATGCGGTATTGATTATATGACACAGGACGGGGAAATGATTGCCACAAGTATTGTTGAATCTGGCCGCTATGCGAACGAGACAGAATCACCTGATGTCATGATATACACAGGTCAAGGTGGAAATCCAAAGGTTTCAAAAGGGGAACCAATAGATCAAAAGCCCGACCGTGGAAACCGTGCACTGATGAATAGCAAGGCAGCAGGAACTCCTGTGAGGGTAATCCGGAGCTATAAATTTCAGGAGGAATCTGGTTATGTTTATGATGGTCTCTACATTGTTGATGATTTTTGGCGAGAAAGAGGGGTTTATGGTAAATTTGTGTTTAGATTTTCGTTGAGGAGGATTTTGGGTCAACCAAAACTCACAGTTTGCAACAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 ko04211 map00310 map04211
Pfam Domains
Protein Families
Pre-SET SAD_SRA SET

Protein Analysis

371

Amino Acids

41.54

Weight (kDa)

9.17

Isoelectric Point (pI)

33.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAD_SRA PF02182 217 - 363 6.7e-47 SAD/SRA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 957
AciI CCGC 2 cut(s) 738, 799
AcoI YGGCCR 1 cut(s) 796
AcsI RAATTY 2 cut(s) 968, 1049
AcuI CTGAAG 2 cut(s) 108, 288
AcyI GRCGYC 1 cut(s) 414
AfaI GTAC 1 cut(s) 590
AfiI CCNNNNNNNGG 1 cut(s) 902
AflII CTTAAG 1 cut(s) 611
AgsI TTSAA 6 cut(s) 13, 259, 596, 734, 791, 870
AjnI CCWGG 2 cut(s) 409, 661
AjuI GAANNNNNNNTTGG 4 cut(s) 362, 394, 491, 523
AluBI AGCT 6 cut(s) 356, 377, 485, 506, 707, 963
AluI AGCT 6 cut(s) 356, 377, 485, 506, 707, 963
Alw21I GWGCWC 1 cut(s) 916
Alw26I GTCTC 3 cut(s) 518, 806, 1007
Alw44I GTGCAC 1 cut(s) 912
Ama87I CYCGRG 1 cut(s) 192
Aor13HI TCCGGA 1 cut(s) 957
AoxI GGCC 3 cut(s) 56, 236, 796
ApaLI GTGCAC 1 cut(s) 912
ApeKI GCWGC 7 cut(s) 118, 307, 383, 436, 512, 599, 932
ApoI RAATTY 2 cut(s) 968, 1049
AspS9I GGNCC 1 cut(s) 236
AsuHPI GGTGA 4 cut(s) 152, 508, 689, 813
AvaI CYCGRG 1 cut(s) 192
BaeGI GKGCMC 1 cut(s) 916
BbsI GAAGAC 1 cut(s) 710
Bbv12I GWGCWC 1 cut(s) 916
BbvI GCAGC 7 cut(s) 130, 319, 370, 448, 499, 611, 944
BccI CCATC 2 cut(s) 532, 992
BciT130I CCWGG 2 cut(s) 411, 663
BcoDI GTCTC 3 cut(s) 518, 806, 1007
BfaI CTAG 2 cut(s) 60, 186
BfrI CTTAAG 1 cut(s) 611
BisI GCNGC 8 cut(s) 119, 308, 384, 437, 513, 600, 799, 933
BlsI GCNGC 8 cut(s) 120, 309, 385, 438, 514, 601, 800, 934
Bme1390I CCNGG 2 cut(s) 411, 663
BmeT110I CYCGRG 1 cut(s) 192
BmgT120I GGNCC 1 cut(s) 236
BmiI GGNNCC 4 cut(s) 111, 285, 408, 879
BmrFI CCNGG 2 cut(s) 411, 663
BmsI GCATC 2 cut(s) 253, 544
BpiI GAAGAC 1 cut(s) 710
BpmI CTGGAG 1 cut(s) 96
BsaHI GRCGYC 1 cut(s) 414
BsaI GGTCTC 1 cut(s) 1007
BsaJI CCNNGG 2 cut(s) 164, 901
BsaWI WCCGGW 1 cut(s) 957
BsaXI ACNNNNNCTCC 2 cut(s) 657, 687
Bsc4I CCNNNNNNNGG 1 cut(s) 902
Bse1I ACTGG 1 cut(s) 113
Bse3DI GCAATG 1 cut(s) 609
BseAI TCCGGA 1 cut(s) 957
BseBI CCWGG 2 cut(s) 411, 663
BseDI CCNNGG 2 cut(s) 164, 901
BseLI CCNNNNNNNGG 1 cut(s) 902
BseMI GCAATG 1 cut(s) 609
BseNI ACTGG 1 cut(s) 113
BseRI GAGGAG 1 cut(s) 1087
BseSI GKGCMC 1 cut(s) 916
BseXI GCAGC 7 cut(s) 130, 319, 370, 448, 499, 611, 944
BsgI GTGCAG 1 cut(s) 137
Bsh1285I CGRYCG 1 cut(s) 901
BshFI GGCC 3 cut(s) 58, 238, 798
BsiEI CGRYCG 1 cut(s) 901
BsiHKAI GWGCWC 1 cut(s) 916
BsiHKCI CYCGRG 1 cut(s) 192
BsiSI CCGG 1 cut(s) 958
BslFI GGGAC 2 cut(s) 27, 668
BslI CCNNNNNNNGG 1 cut(s) 902
BsmAI GTCTC 3 cut(s) 518, 806, 1007
BsmFI GGGAC 2 cut(s) 27, 668
BsmI GAATGC 3 cut(s) 305, 434, 554
BsnI GGCC 3 cut(s) 58, 238, 798
Bso31I GGTCTC 1 cut(s) 1007
BsoBI CYCGRG 1 cut(s) 192
Bsp1286I GDGCHC 1 cut(s) 916
Bsp13I TCCGGA 1 cut(s) 957
Bsp143I GATC 1 cut(s) 886
BspACI CCGC 2 cut(s) 738, 799
BspANI GGCC 3 cut(s) 58, 238, 798
BspEI TCCGGA 1 cut(s) 957
BspHI TCATGA 1 cut(s) 831
BspLI GGNNCC 4 cut(s) 111, 285, 408, 879
BspTI CTTAAG 1 cut(s) 611
BspTNI GGTCTC 1 cut(s) 1007
BsrDI GCAATG 1 cut(s) 609
BsrI ACTGG 1 cut(s) 113
BssECI CCNNGG 2 cut(s) 164, 901
BssMI GATC 1 cut(s) 886
BssNI GRCGYC 1 cut(s) 414
BssT1I CCWWGG 1 cut(s) 164
Bst2UI CCWGG 2 cut(s) 411, 663
Bst4CI ACNGT 6 cut(s) 73, 244, 902, 911, 1102, 1112
Bst6I CTCTTC 3 cut(s) 129, 264, 269
BstACI GRCGYC 1 cut(s) 414
BstAFI CTTAAG 1 cut(s) 611
BstC8I GCNNGC 2 cut(s) 305, 434
BstDSI CCRYGG 1 cut(s) 901
BstKTI GATC 1 cut(s) 889
BstMAI GTCTC 3 cut(s) 518, 806, 1007
BstMBI GATC 1 cut(s) 886
BstMCI CGRYCG 1 cut(s) 901
BstMWI GCNNNNNNNGC 3 cut(s) 374, 383, 512
BstNI CCWGG 2 cut(s) 411, 663
BstSCI CCNGG 2 cut(s) 409, 661
BstSLI GKGCMC 1 cut(s) 916
BstV1I GCAGC 7 cut(s) 130, 319, 370, 448, 499, 611, 944
BstV2I GAAGAC 1 cut(s) 710
BsuRI GGCC 3 cut(s) 58, 238, 798
BtgI CCRYGG 1 cut(s) 901
BtgZI GCGATG 1 cut(s) 567
BtsIMutI CAGTG 2 cut(s) 120, 914
Cac8I GCNNGC 2 cut(s) 305, 434
CciI TCATGA 1 cut(s) 831
Cfr13I GGNCC 1 cut(s) 236
CseI GACGC 1 cut(s) 403
Csp6I GTAC 1 cut(s) 589
CspCI CAANNNNNGTGG 2 cut(s) 766, 801
CviAII CATG 1 cut(s) 832
CviQI GTAC 1 cut(s) 589
DpnI GATC 1 cut(s) 888
DpnII GATC 1 cut(s) 886
EaeI YGGCCR 1 cut(s) 796
Eam1104I CTCTTC 3 cut(s) 129, 264, 269
EarI CTCTTC 3 cut(s) 129, 264, 269
Eco130I CCWWGG 1 cut(s) 164
Eco147I AGGCCT 1 cut(s) 58
Eco31I GGTCTC 1 cut(s) 1007
Eco57I CTGAAG 2 cut(s) 108, 288
Eco88I CYCGRG 1 cut(s) 192
EcoRII CCWGG 2 cut(s) 409, 661
EcoT14I CCWWGG 1 cut(s) 164
ErhI CCWWGG 1 cut(s) 164
FaeI CATG 1 cut(s) 835
FaqI GGGAC 2 cut(s) 27, 668
FatI CATG 1 cut(s) 831
Fnu4HI GCNGC 8 cut(s) 119, 308, 384, 437, 513, 600, 799, 933
Fsp4HI GCNGC 8 cut(s) 119, 308, 384, 437, 513, 600, 799, 933
FspBI CTAG 2 cut(s) 60, 186
GluI GCNGC 8 cut(s) 119, 308, 384, 437, 513, 600, 799, 933
GsuI CTGGAG 1 cut(s) 96
HaeIII GGCC 3 cut(s) 58, 238, 798
HapII CCGG 1 cut(s) 958
HgaI GACGC 1 cut(s) 403
Hin1I GRCGYC 1 cut(s) 414
Hin1II CATG 1 cut(s) 835
HincII GTYRAC 2 cut(s) 634, 1088
HindII GTYRAC 2 cut(s) 634, 1088
HindIII AAGCTT 2 cut(s) 375, 504
HinfI GANTC 3 cut(s) 791, 818, 980
HpaI GTTAAC 1 cut(s) 634
HpaII CCGG 1 cut(s) 958
HphI GGTGA 4 cut(s) 152, 508, 689, 813
Hpy166II GTNNAC 4 cut(s) 98, 634, 914, 1088
Hpy188III TCNNGA 8 cut(s) 77, 215, 287, 392, 521, 832, 958, 974
Hpy8I GTNNAC 4 cut(s) 98, 634, 914, 1088
Hpy99I CGWCG 1 cut(s) 244
HpyAV CCTTC 2 cut(s) 83, 664
HpyCH4III ACNGT 6 cut(s) 73, 244, 902, 911, 1102, 1112
HpyCH4IV ACGT 2 cut(s) 291, 591
HpyCH4V TGCA 7 cut(s) 118, 338, 386, 467, 515, 914, 1107
HpyF10VI GCNNNNNNNGC 3 cut(s) 374, 383, 512
HpySE526I ACGT 2 cut(s) 291, 591
Hsp92I GRCGYC 1 cut(s) 414
Hsp92II CATG 1 cut(s) 835
Kpn2I TCCGGA 1 cut(s) 957
KspAI GTTAAC 1 cut(s) 634
Kzo9I GATC 1 cut(s) 886
LmnI GCTCC 3 cut(s) 115, 182, 960
Lsp1109I GCAGC 7 cut(s) 130, 319, 370, 448, 499, 611, 944
LweI GCATC 2 cut(s) 253, 544
MaeI CTAG 2 cut(s) 60, 186
MaeII ACGT 2 cut(s) 291, 591
MaeIII GTNAC 5 cut(s) 4, 320, 449, 677, 725
MalI GATC 1 cut(s) 888
MboI GATC 1 cut(s) 886
MboII GAAGA 9 cut(s) 146, 158, 243, 281, 286, 382, 511, 628, 710
MhlI GDGCHC 1 cut(s) 916
MluCI AATT 7 cut(s) 46, 172, 559, 584, 701, 968, 1049
MmeI TCCRAC 1 cut(s) 560
MnlI CCTC 8 cut(s) 48, 130, 201, 942, 970, 1028, 1065, 1068
MroI TCCGGA 1 cut(s) 957
MseI TTAA 5 cut(s) 51, 294, 612, 633, 1114
MspCI CTTAAG 1 cut(s) 611
MspI CCGG 1 cut(s) 958
MspR9I CCNGG 2 cut(s) 411, 663
Mva1269I GAATGC 3 cut(s) 305, 434, 554
MvaI CCWGG 2 cut(s) 411, 663
MwoI GCNNNNNNNGC 3 cut(s) 374, 383, 512
NdeII GATC 1 cut(s) 886
NlaIII CATG 1 cut(s) 835
NlaIV GGNNCC 4 cut(s) 111, 285, 408, 879
NmuCI GTSAC 4 cut(s) 320, 449, 677, 725
PaeR7I CTCGAG 1 cut(s) 192
PagI TCATGA 1 cut(s) 831
PceI AGGCCT 1 cut(s) 58
PctI GAATGC 3 cut(s) 305, 434, 554
PfeI GAWTC 3 cut(s) 791, 818, 980
PfoI TCCNGGA 1 cut(s) 661
PkrI GCNGC 8 cut(s) 120, 309, 385, 438, 514, 601, 800, 934
Psp6I CCWGG 2 cut(s) 409, 661
PspGI CCWGG 2 cut(s) 409, 661
PspN4I GGNNCC 4 cut(s) 111, 285, 408, 879
PspPI GGNCC 1 cut(s) 236
PspXI VCTCGAGB 1 cut(s) 192
RsaI GTAC 1 cut(s) 590
RsaNI GTAC 1 cut(s) 589
SaqAI TTAA 5 cut(s) 51, 294, 612, 633, 1114
SatI GCNGC 8 cut(s) 119, 308, 384, 437, 513, 600, 799, 933
Sau3AI GATC 1 cut(s) 886
Sau96I GGNCC 1 cut(s) 236
ScrFI CCNGG 2 cut(s) 411, 663
SduI GDGCHC 1 cut(s) 916
SfaNI GCATC 2 cut(s) 253, 544
Sfr274I CTCGAG 1 cut(s) 192
SlaI CTCGAG 1 cut(s) 192
SmlI CTYRAG 2 cut(s) 192, 611
SmoI CTYRAG 2 cut(s) 192, 611
Sse9I AATT 7 cut(s) 46, 172, 559, 584, 701, 968, 1049
SseBI AGGCCT 1 cut(s) 58
SsiI CCGC 2 cut(s) 738, 799
SspMI CTAG 2 cut(s) 60, 186
StuI AGGCCT 1 cut(s) 58
StyD4I CCNGG 2 cut(s) 409, 661
StyI CCWWGG 1 cut(s) 164
TaaI ACNGT 6 cut(s) 73, 244, 902, 911, 1102, 1112
TaiI ACGT 2 cut(s) 294, 594
TaqI TCGA 3 cut(s) 76, 193, 214
TasI AATT 7 cut(s) 46, 172, 559, 584, 701, 968, 1049
TauI GCSGC 1 cut(s) 801
TfiI GAWTC 3 cut(s) 791, 818, 980
Tru1I TTAA 5 cut(s) 51, 294, 612, 633, 1114
Tru9I TTAA 5 cut(s) 51, 294, 612, 633, 1114
TscAI CASTG 2 cut(s) 120, 921
TseFI GTSAC 4 cut(s) 320, 449, 677, 725
TseI GCWGC 7 cut(s) 118, 307, 383, 436, 512, 599, 932
Tsp45I GTSAC 4 cut(s) 320, 449, 677, 725
TspDTI ATGAA 4 cut(s) 159, 167, 710, 935
TspRI CASTG 2 cut(s) 120, 921
Vha464I CTTAAG 1 cut(s) 611
VneI GTGCAC 1 cut(s) 912
XapI RAATTY 2 cut(s) 968, 1049
XhoI CTCGAG 1 cut(s) 192
XspI CTAG 2 cut(s) 60, 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.