FvH4_2g33321

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001634
Physical Location & Seq
Forward (+)
25037656 .. 25040995
3340 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33321.t3

Sequence Viewer

Length: 2301 bp
ATGAAGAAGTTCATGCTTCTGGTAACGCTTATGGTTCTGGCTATCACTCACAACTGTGAGGTGGTAGCAGAGAAGAGTGAGCATGTGAAAAAGACGTACATCATTCACATGGACAAGTCCCTAATGCCTACGAGTTTCGAAGACGACCATGTCAACTGGTACAGCTCGTCGTTGAAAGCAGTCACCGATTCAGCTGACATGCTTTACACTTACAAGACTGTCATCCATGGCTACTCCACGAGGCTGACTGCCGAGGAAGCTGAACTTCTCGAAAAGCAGCCAGGGATTCTCTCTGTCCTGCCGGAGAGGAGGTACGAGCTTCACACAACTAGGACTCCGGAGTTTCTTGGACTAGGCAAAAGCGAAGCTGTGTTTCCGGCTTCGGATATGGTGAGTGAGGTGATCATCGGAGTGGTGGACACCGGCGTGTGGCCGGAGCACAAAAGCTACGACGACACTGGTCTTGGACCAGTGCCGAGTGGCTGGAAAGGGACGTGCCAGCAGGGTAAGACGTTCAACTCTTCGAGCTGCAACAAGAAGCTGATTGGTGCTACGTTTTTCTCAAACGGGTACGAAGCAGCGTTTGGACCTATTGACGAAAGCGTTGAATCGAAGTCACCGAGAGACGACGATGGCCACGGAACGCACACCTCAACCACAGCGGCGGGGTCAGTTGTTACAGGAGCCAGCTTTCTTGGTTATGCTCCAGGCACAGCTAGGGGTATGGCGCCACAAGCTCGAATAGCTACATACAAGGCTTGCTGGCTTGGAGGGTGCTTCGGTTCTGATATCATAGCTGCAATGGAGAAGGCTGTTGAGGATGGGGTTAATGTTTTGTCTTTGTCTATTGGTGGATCGCAGGTTGATTATTATAGAGACACGGTTGCCATTGGAGCTTTCTCTGCGGCGGCGCAGGGTATTCTAGTGTCGTGTTCGGCGGGAAATGGTGGACCGGAGGCGGGAAGCTTGTCCAATGTTGCGCCTTGGATAACCACTGTGGGCGCTGGAACATTGGACAGGGACTTCCCGGCTTATGTCAGCCTTGGAAGTGCGAAGAAGTATGCAGGTATATCTCTATACAGGGGAACGGCCTTATCTAGTGGATTGATTCCACTTATAGATGCTCGCAACGCGAGCAGTTCAAGTGATGATCTTTGCAGTCCAGACAGTTTGATTCCGGCAAAGGTTGCCGGGAAGATTGTGGTGTGTGACAGAGGGGGAACTCCGAGGGTTCAAAAGAGTCTGGTGGTGAAAAAGGCTGGTGGTGTGGGGATGATTTTGACCAACACCGAAACATTTGGAGAAGAGCTTGTTGCTGATGCGTATTTCCTTCCTACAGCAGCAGTTGGCCAGAAAGCTGGTGACGCAATAAAGAGCTATATTGCATCAGATTCTAATCCCACAGCAACAATTACTCTTGGAGAAACAAAGTTAGGCGTTCAACCTTCACCGGTGGTGGCTGCGTTTAGCTCTAGAGGACCAAATTTTGTCACTCCAGAAGTGCTCAAACCAGACCTGATAGCACCAGGAATGAACATCCTAGCTGGGTGGACTGGCGCAGTTGGACCAACTGGACTTCCTGAAGACAAGAGGAGGGTGAACTTCAACATCATTTCAGGTACATCCATGTCGTGCCCACACGTGAGTGGACTAGCGGCCCTTGTCAAGGCGGCTCACCCTGACTGGAGTCCTGCAGCCATCAAGTCTGCTCTCATGACCACAGCCTATTCAAACTACAAAACTGGAGAAACAATCAGAGATGTTTCTACCGGAAGTGCTGCAACACCTTTCGATTATGGTGCTGGGCATGTGGATCCAGTAACAGCTCTTGACCCTGGCCTTGTCTATGATCTTGCTGTGGAGGACTACCTAAGTTTCCTATGTGCCTTGAATTATTCCGCTTCTCAGATCAAGCAAACCACTAAGAAGGACTTTACCTGTGGCTCAAGCAAGAAATATAGCCTCGGAGATTTGAATTACCCCTCCTTTTCTCTTGCTCTGGATACATCTTCAGGCCAAGGGGGTGGTACTGGTGCATCAACCACTCTTAAGTATACAAGGACTCTGACCAATGTGGGTGCCCCGGCAACATACAAGGTCTCAGTGTCTTCACTGACCCCAGCAGTGAAGATATCTGTAGAACCAGGAACACTAAGTTTCAGTGATTCATATGAGAAGAAGACATACACTGTGACTTTTGTTGCTAGCTCTTTGCCATCCGGCACAGCCAGCTTCGGTCGCTTGGAATGGTCTGATGGGAAACACATTGTAGGTAGTCATATTGCTTACACCTGGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

767

Amino Acids

80.39

Weight (kDa)

5.92

Isoelectric Point (pI)

29.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 82 - 551 2e-48 Subtilase family
PA PF02225 331 - 406 9.9e-14 PA domain
fn3_6 PF17766 606 - 711 1.3e-23 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 149
Acc36I ACCTGC 2 cut(s) 850, 1055
AccB1I GGYRCC 2 cut(s) 727, 2078
AccI GTMKAC 1 cut(s) 2054
AccII CGCG 1 cut(s) 1133
AccIII TCCGGA 1 cut(s) 337
AciI CCGC 9 cut(s) 662, 665, 905, 908, 938, 959, 1655, 1670, 1899
AclWI GGATC 3 cut(s) 862, 1808, 1821
AcoI YGGCCR 3 cut(s) 431, 634, 1348
AcsI RAATTY 1 cut(s) 1483
AcuI CTGAAG 2 cut(s) 1602, 1995
AcvI CACGTG 1 cut(s) 1642
AcyI GRCGYC 1 cut(s) 728
AfaI GTAC 6 cut(s) 98, 161, 314, 572, 1621, 2029
AfiI CCNNNNNNNGG 3 cut(s) 429, 959, 1666
AflII CTTAAG 1 cut(s) 2048
AflIII ACRYGT 1 cut(s) 1639
AgeI ACCGGT 1 cut(s) 1450
AjiI CACGTC 1 cut(s) 495
AjnI CCWGG 6 cut(s) 280, 706, 1525, 1834, 2143, 2291
AjuI GAANNNNNNNTTGG 2 cut(s) 567, 599
AleI CACNNNNGTG 3 cut(s) 54, 425, 1644
Alw21I GWGCWC 2 cut(s) 441, 1506
Alw26I GTCTC 3 cut(s) 618, 870, 2104
AlwI GGATC 3 cut(s) 862, 1808, 1821
Aor13HI TCCGGA 1 cut(s) 337
AoxI GGCC 7 cut(s) 431, 634, 1089, 1348, 1656, 1837, 2014
ApeKI GCWGC 8 cut(s) 277, 528, 578, 797, 1340, 1460, 1694, 1778
ApoI RAATTY 1 cut(s) 1483
AsiGI ACCGGT 1 cut(s) 1450
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 5 cut(s) 730, 913, 982, 1004, 1559
AspS9I GGNCC 6 cut(s) 467, 587, 950, 1478, 1565, 1657
AsuC2I CCSGG 3 cut(s) 1028, 1192, 2084
AsuHPI GGTGA 9 cut(s) 175, 403, 412, 609, 1261, 1373, 1440, 1609, 1667
AsuII TTCGAA 1 cut(s) 138
AsuNHI GCTAGC 1 cut(s) 2204
AvaII GGWCC 5 cut(s) 467, 587, 950, 1478, 1565
BaeGI GKGCMC 2 cut(s) 1637, 2083
BalI TGGCCA 2 cut(s) 636, 1350
BamHI GGATCC 1 cut(s) 1813
BanI GGYRCC 2 cut(s) 727, 2078
BarI GAAGNNNNNNTAC 2 cut(s) 2168, 2200
BauI CACGAG 1 cut(s) 238
BbrPI CACGTG 1 cut(s) 1642
BbsI GAAGAC 4 cut(s) 147, 1590, 2100, 2186
Bbv12I GWGCWC 2 cut(s) 441, 1506
BbvI GCAGC 8 cut(s) 289, 515, 590, 784, 1352, 1447, 1706, 1765
BccI CCATC 5 cut(s) 626, 815, 1706, 2224, 2249
BceAI ACGGC 1 cut(s) 1104
BcgI CGANNNNNNTGC 2 cut(s) 1781, 1815
BciT130I CCWGG 6 cut(s) 282, 708, 1527, 1836, 2145, 2293
BciVI GTATCC 1 cut(s) 1996
BclI TGATCA 1 cut(s) 402
BcnI CCSGG 3 cut(s) 1028, 1192, 2084
BcoDI GTCTC 3 cut(s) 618, 870, 2104
BfaI CTAG 9 cut(s) 330, 353, 717, 923, 1098, 1473, 1541, 1652, 2205
BfmI CTRYAG 3 cut(s) 1335, 1692, 2136
BfoI RGCGCY 2 cut(s) 731, 1005
BfrI CTTAAG 1 cut(s) 2048
BfuAI ACCTGC 2 cut(s) 850, 1055
BfuI GTATCC 1 cut(s) 1996
Bme1390I CCNGG 9 cut(s) 282, 708, 1028, 1192, 1527, 1836, 2084, 2145, 2293
Bme18I GGWCC 5 cut(s) 467, 587, 950, 1478, 1565
BmgBI CACGTC 1 cut(s) 495
BmgT120I GGNCC 6 cut(s) 467, 587, 950, 1478, 1565, 1657
BmiI GGNNCC 4 cut(s) 685, 729, 1815, 2080
BmrFI CCNGG 9 cut(s) 282, 708, 1028, 1192, 1527, 1836, 2084, 2145, 2293
BmsI GCATC 4 cut(s) 1111, 1309, 1394, 2045
BmtI GCTAGC 1 cut(s) 2208
BoxI GACNNNNGTC 2 cut(s) 459, 1686
BpiI GAAGAC 4 cut(s) 147, 1590, 2100, 2186
BpmI CTGGAG 4 cut(s) 690, 1479, 1705, 1764
Bpu14I TTCGAA 1 cut(s) 138
BpuEI CTTGAG 1 cut(s) 1930
BpuMI CCSGG 3 cut(s) 1028, 1192, 2084
BsaAI YACGTR 1 cut(s) 1642
BsaBI GATNNNNATC 1 cut(s) 1395
BsaHI GRCGYC 1 cut(s) 728
BsaI GGTCTC 1 cut(s) 2104
BsaWI WCCGGW 4 cut(s) 337, 952, 1450, 1769
BsaXI ACNNNNNCTCC 4 cut(s) 296, 319, 326, 349
Bsc4I CCNNNNNNNGG 3 cut(s) 429, 959, 1666
Bse118I RCCGGY 2 cut(s) 422, 1450
Bse1I ACTGG 9 cut(s) 161, 463, 470, 1558, 1576, 1688, 1747, 1817, 2035
Bse3DI GCAATG 1 cut(s) 807
Bse8I GATNNNNATC 1 cut(s) 1395
BseAI TCCGGA 1 cut(s) 337
BseBI CCWGG 6 cut(s) 282, 708, 1527, 1836, 2145, 2293
BseGI GGATG 6 cut(s) 222, 826, 1278, 1536, 1622, 2216
BseJI GATNNNNATC 1 cut(s) 1395
BseLI CCNNNNNNNGG 3 cut(s) 429, 959, 1666
BseMI GCAATG 1 cut(s) 807
BseMII CTCAG 2 cut(s) 1919, 2115
BseNI ACTGG 9 cut(s) 161, 463, 470, 1558, 1576, 1688, 1747, 1817, 2035
BseRI GAGGAG 2 cut(s) 322, 1606
BseSI GKGCMC 2 cut(s) 1637, 2083
BseXI GCAGC 8 cut(s) 289, 515, 590, 784, 1352, 1447, 1706, 1765
BseYI CCCAGC 3 cut(s) 1544, 1802, 2119
Bsh1236I CGCG 1 cut(s) 1133
Bsh1285I CGRYCG 1 cut(s) 2239
BshFI GGCC 7 cut(s) 433, 636, 1091, 1350, 1658, 1839, 2016
BshNI GGYRCC 2 cut(s) 727, 2078
BshTI ACCGGT 1 cut(s) 1450
BsiEI CGRYCG 1 cut(s) 2239
BsiHKAI GWGCWC 2 cut(s) 441, 1506
BslFI GGGAC 3 cut(s) 103, 505, 1034
BslI CCNNNNNNNGG 3 cut(s) 429, 959, 1666
BsmAI GTCTC 3 cut(s) 618, 870, 2104
BsmBI CGTCTC 1 cut(s) 618
BsmFI GGGAC 3 cut(s) 103, 505, 1034
BsnI GGCC 7 cut(s) 433, 636, 1091, 1350, 1658, 1839, 2016
Bso31I GGTCTC 1 cut(s) 2104
Bsp119I TTCGAA 1 cut(s) 138
Bsp1286I GDGCHC 4 cut(s) 441, 1506, 1637, 2083
Bsp13I TCCGGA 1 cut(s) 337
Bsp143I GATC 6 cut(s) 402, 854, 1150, 1813, 1849, 1908
Bsp19I CCATGG 1 cut(s) 226
BspACI CCGC 9 cut(s) 662, 665, 905, 908, 938, 959, 1655, 1670, 1899
BspANI GGCC 7 cut(s) 433, 636, 1091, 1350, 1658, 1839, 2016
BspCNI CTCAG 2 cut(s) 1918, 2114
BspEI TCCGGA 1 cut(s) 337
BspFNI CGCG 1 cut(s) 1133
BspHI TCATGA 1 cut(s) 1713
BspLI GGNNCC 4 cut(s) 685, 729, 1815, 2080
BspMAI CTGCAG 1 cut(s) 1696
BspMI ACCTGC 2 cut(s) 850, 1055
BspOI GCTAGC 1 cut(s) 2208
BspPI GGATC 3 cut(s) 862, 1808, 1821
BspQI GCTCTTC 1 cut(s) 1299
BspT104I TTCGAA 1 cut(s) 138
BspT107I GGYRCC 2 cut(s) 727, 2078
BspTI CTTAAG 1 cut(s) 2048
BspTNI GGTCTC 1 cut(s) 2104
BsrDI GCAATG 1 cut(s) 807
BsrFI RCCGGY 2 cut(s) 422, 1450
BsrI ACTGG 9 cut(s) 161, 463, 470, 1558, 1576, 1688, 1747, 1817, 2035
BssAI RCCGGY 2 cut(s) 422, 1450
BssMI GATC 6 cut(s) 402, 854, 1150, 1813, 1849, 1908
BssNAI GTATAC 1 cut(s) 2055
BssNI GRCGYC 1 cut(s) 728
BssSI CACGAG 1 cut(s) 238
BssT1I CCWWGG 4 cut(s) 226, 983, 1042, 2017
Bst1107I GTATAC 1 cut(s) 2055
Bst2BI CACGAG 1 cut(s) 238
Bst2UI CCWGG 6 cut(s) 282, 708, 1527, 1836, 2145, 2293
Bst4CI ACNGT 6 cut(s) 56, 220, 883, 997, 1169, 2191
Bst6I CTCTTC 3 cut(s) 68, 526, 1299
BstACI GRCGYC 1 cut(s) 728
BstAFI CTTAAG 1 cut(s) 2048
BstAPI GCANNNNNTGC 1 cut(s) 1187
BstBAI YACGTR 1 cut(s) 1642
BstBI TTCGAA 1 cut(s) 138
BstC8I GCNNGC 8 cut(s) 500, 688, 760, 764, 1126, 1135, 2206, 2230
BstDEI CTNAG 5 cut(s) 1871, 1905, 1923, 2101, 2153
BstDSI CCRYGG 2 cut(s) 226, 637
BstENI CCTNNNNNAGG 1 cut(s) 1664
BstF5I GGATG 6 cut(s) 222, 826, 1278, 1536, 1622, 2216
BstFNI CGCG 1 cut(s) 1133
BstH2I RGCGCY 2 cut(s) 731, 1005
BstHHI GCGC 5 cut(s) 730, 913, 982, 1004, 1559
BstKTI GATC 6 cut(s) 405, 857, 1153, 1816, 1852, 1911
BstMAI GTCTC 3 cut(s) 618, 870, 2104
BstMBI GATC 6 cut(s) 402, 854, 1150, 1813, 1849, 1908
BstMCI CGRYCG 1 cut(s) 2239
BstNI CCWGG 6 cut(s) 282, 708, 1527, 1836, 2145, 2293
BstNSI RCATGY 3 cut(s) 86, 202, 1811
BstPAI GACNNNNGTC 2 cut(s) 459, 1686
BstSCI CCNGG 9 cut(s) 280, 706, 1026, 1190, 1525, 1834, 2082, 2143, 2291
BstSFI CTRYAG 3 cut(s) 1335, 1692, 2136
BstSLI GKGCMC 2 cut(s) 1637, 2083
BstUI CGCG 1 cut(s) 1133
BstV1I GCAGC 8 cut(s) 289, 515, 590, 784, 1352, 1447, 1706, 1765
BstV2I GAAGAC 4 cut(s) 147, 1590, 2100, 2186
BstX2I RGATCY 1 cut(s) 1813
BstXI CCANNNNNNTGG 2 cut(s) 1358, 2024
BstYI RGATCY 1 cut(s) 1813
BstZ17I GTATAC 1 cut(s) 2055
BsuI GTATCC 1 cut(s) 1996
BsuRI GGCC 7 cut(s) 433, 636, 1091, 1350, 1658, 1839, 2016
BtgI CCRYGG 2 cut(s) 226, 637
BtrI CACGTC 1 cut(s) 495
BtsCI GGATG 6 cut(s) 222, 826, 1278, 1536, 1622, 2216
BtsI GCAGTG 1 cut(s) 2130
BtsIMutI CAGTG 8 cut(s) 456, 477, 993, 2109, 2111, 2130, 2167, 2187
BveI ACCTGC 2 cut(s) 850, 1055
Cac8I GCNNGC 8 cut(s) 500, 688, 760, 764, 1126, 1135, 2206, 2230
CciI TCATGA 1 cut(s) 1713
CfoI GCGC 5 cut(s) 730, 913, 982, 1004, 1559
Cfr10I RCCGGY 2 cut(s) 422, 1450
Cfr13I GGNCC 6 cut(s) 467, 587, 950, 1478, 1565, 1657
CseI GACGC 1 cut(s) 1373
Csp6I GTAC 6 cut(s) 97, 160, 313, 571, 1620, 2028
CspAI ACCGGT 1 cut(s) 1450
CviAII CATG 9 cut(s) 13, 83, 109, 149, 199, 227, 1627, 1714, 1808
CviQI GTAC 6 cut(s) 97, 160, 313, 571, 1620, 2028
DdeI CTNAG 5 cut(s) 1871, 1905, 1923, 2101, 2153
DinI GGCGCC 1 cut(s) 729
DpnI GATC 6 cut(s) 404, 856, 1152, 1815, 1851, 1910
DpnII GATC 6 cut(s) 402, 854, 1150, 1813, 1849, 1908
DrdI GACNNNNNNGTC 1 cut(s) 149
DseDI GACNNNNNNGTC 1 cut(s) 149
EaeI YGGCCR 3 cut(s) 431, 634, 1348
Eam1104I CTCTTC 3 cut(s) 68, 526, 1299
EarI CTCTTC 3 cut(s) 68, 526, 1299
Eco130I CCWWGG 4 cut(s) 226, 983, 1042, 2017
Eco31I GGTCTC 1 cut(s) 2104
Eco32I GATATC 2 cut(s) 790, 2133
Eco47I GGWCC 5 cut(s) 467, 587, 950, 1478, 1565
Eco57I CTGAAG 2 cut(s) 1602, 1995
Eco72I CACGTG 1 cut(s) 1642
EcoNI CCTNNNNNAGG 1 cut(s) 1664
EcoRII CCWGG 6 cut(s) 280, 706, 1525, 1834, 2143, 2291
EcoRV GATATC 2 cut(s) 790, 2133
EcoT14I CCWWGG 4 cut(s) 226, 983, 1042, 2017
EgeI GGCGCC 1 cut(s) 729
EheI GGCGCC 1 cut(s) 729
ErhI CCWWGG 4 cut(s) 226, 983, 1042, 2017
Esp3I CGTCTC 1 cut(s) 618
FaeI CATG 9 cut(s) 16, 86, 112, 152, 202, 230, 1630, 1717, 1811
FalI AAGNNNNNCTT 4 cut(s) 249, 281, 1916, 1948
FaqI GGGAC 3 cut(s) 103, 505, 1034
FatI CATG 9 cut(s) 12, 82, 108, 148, 198, 226, 1626, 1713, 1807
FauI CCCGC 3 cut(s) 658, 931, 952
FauNDI CATATG 1 cut(s) 2170
FbaI TGATCA 1 cut(s) 402
FblI GTMKAC 1 cut(s) 2054
FokI GGATG 6 cut(s) 209, 833, 1285, 1523, 1609, 2203
FspBI CTAG 9 cut(s) 330, 353, 717, 923, 1098, 1473, 1541, 1652, 2205
GlaI GCGC 5 cut(s) 729, 912, 981, 1003, 1558
GsaI CCCAGC 3 cut(s) 1548, 1806, 2123
GsuI CTGGAG 4 cut(s) 690, 1479, 1705, 1764
HaeII RGCGCY 2 cut(s) 731, 1005
HaeIII GGCC 7 cut(s) 433, 636, 1091, 1350, 1658, 1839, 2016
HgaI GACGC 1 cut(s) 1373
HhaI GCGC 5 cut(s) 730, 913, 982, 1004, 1559
Hin1I GRCGYC 1 cut(s) 728
Hin1II CATG 9 cut(s) 16, 86, 112, 152, 202, 230, 1630, 1717, 1811
Hin6I GCGC 5 cut(s) 728, 911, 980, 1002, 1557
HinP1I GCGC 5 cut(s) 728, 911, 980, 1002, 1557
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HindIII AAGCTT 1 cut(s) 964
HphI GGTGA 9 cut(s) 175, 403, 412, 609, 1261, 1373, 1440, 1609, 1667
Hpy166II GTNNAC 7 cut(s) 154, 418, 950, 1551, 1600, 1649, 2055
Hpy188III TCNNGA 9 cut(s) 269, 338, 1163, 1473, 1496, 1580, 1714, 1829, 2000
Hpy8I GTNNAC 7 cut(s) 154, 418, 950, 1551, 1600, 1649, 2055
Hpy99I CGWCG 3 cut(s) 172, 455, 632
HpyAV CCTTC 4 cut(s) 802, 1340, 1455, 1921
HpyCH4III ACNGT 6 cut(s) 56, 220, 883, 997, 1169, 2191
HpyCH4IV ACGT 5 cut(s) 95, 494, 512, 554, 1641
HpyCH4V TGCA 8 cut(s) 531, 800, 1064, 1158, 1385, 1694, 1781, 2036
HpyF3I CTNAG 5 cut(s) 1871, 1905, 1923, 2101, 2153
HpySE526I ACGT 5 cut(s) 95, 494, 512, 554, 1641
Hsp92I GRCGYC 1 cut(s) 728
Hsp92II CATG 9 cut(s) 16, 86, 112, 152, 202, 230, 1630, 1717, 1811
HspAI GCGC 5 cut(s) 728, 911, 980, 1002, 1557
KasI GGCGCC 1 cut(s) 727
Kpn2I TCCGGA 1 cut(s) 337
Ksp22I TGATCA 1 cut(s) 402
Kzo9I GATC 6 cut(s) 402, 854, 1150, 1813, 1849, 1908
LguI GCTCTTC 1 cut(s) 1299
LmnI GCTCC 4 cut(s) 436, 683, 709, 893
Lsp1109I GCAGC 8 cut(s) 289, 515, 590, 784, 1352, 1447, 1706, 1765
LweI GCATC 4 cut(s) 1111, 1309, 1394, 2045
MaeI CTAG 9 cut(s) 330, 353, 717, 923, 1098, 1473, 1541, 1652, 2205
MaeII ACGT 5 cut(s) 95, 494, 512, 554, 1641
MaeIII GTNAC 9 cut(s) 22, 181, 615, 676, 1208, 1361, 1489, 1819, 2191
MalI GATC 6 cut(s) 404, 856, 1152, 1815, 1851, 1910
MboI GATC 6 cut(s) 402, 854, 1150, 1813, 1849, 1908
MflI RGATCY 1 cut(s) 1813
MhlI GDGCHC 4 cut(s) 441, 1506, 1637, 2083
MlsI TGGCCA 2 cut(s) 636, 1350
MluCI AATT 4 cut(s) 1410, 1483, 1891, 1975
MluNI TGGCCA 2 cut(s) 636, 1350
Mly113I GGCGCC 1 cut(s) 728
MlyI GAGTC 4 cut(s) 328, 1249, 1696, 2056
MmeI TCCRAC 1 cut(s) 1543
Mox20I TGGCCA 2 cut(s) 636, 1350
MroI TCCGGA 1 cut(s) 337
MroXI GAANNNNTTC 1 cut(s) 8
MscI TGGCCA 2 cut(s) 636, 1350
MseI TTAA 2 cut(s) 828, 2049
MslI CAYNNNNRTG 5 cut(s) 54, 107, 410, 425, 1644
Msp20I TGGCCA 2 cut(s) 636, 1350
MspA1I CMGCKG 2 cut(s) 194, 662
MspCI CTTAAG 1 cut(s) 2048
MspR9I CCNGG 9 cut(s) 282, 708, 1028, 1192, 1527, 1836, 2084, 2145, 2293
MvaI CCWGG 6 cut(s) 282, 708, 1527, 1836, 2145, 2293
MvnI CGCG 1 cut(s) 1133
NarI GGCGCC 1 cut(s) 728
NciI CCSGG 3 cut(s) 1028, 1192, 2084
NcoI CCATGG 1 cut(s) 226
NdeI CATATG 1 cut(s) 2170
NdeII GATC 6 cut(s) 402, 854, 1150, 1813, 1849, 1908
NheI GCTAGC 1 cut(s) 2204
NlaIII CATG 9 cut(s) 16, 86, 112, 152, 202, 230, 1630, 1717, 1811
NlaIV GGNNCC 4 cut(s) 685, 729, 1815, 2080
NmeAIII GCCGAG 2 cut(s) 277, 501
NmuCI GTSAC 6 cut(s) 181, 615, 1208, 1361, 1489, 2191
NspI RCATGY 3 cut(s) 86, 202, 1811
NspV TTCGAA 1 cut(s) 138
OliI CACNNNNGTG 3 cut(s) 54, 425, 1644
PagI TCATGA 1 cut(s) 1713
PciSI GCTCTTC 1 cut(s) 1299
PcsI WCGNNNNNNNCGW 1 cut(s) 617
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 7 cut(s) 188, 286, 608, 1108, 1174, 1391, 2165
PflFI GACNNNGTC 1 cut(s) 149
PinAI ACCGGT 1 cut(s) 1450
PleI GAGTC 4 cut(s) 328, 1248, 1695, 2056
PluTI GGCGCC 1 cut(s) 731
PmaCI CACGTG 1 cut(s) 1642
PmlI CACGTG 1 cut(s) 1642
PpsI GAGTC 4 cut(s) 328, 1248, 1695, 2056
Ppu21I YACGTR 1 cut(s) 1642
PshAI GACNNNNGTC 2 cut(s) 459, 1686
Psp6I CCWGG 6 cut(s) 280, 706, 1525, 1834, 2143, 2291
PspCI CACGTG 1 cut(s) 1642
PspFI CCCAGC 3 cut(s) 1544, 1802, 2119
PspGI CCWGG 6 cut(s) 280, 706, 1525, 1834, 2143, 2291
PspN4I GGNNCC 4 cut(s) 685, 729, 1815, 2080
PspPI GGNCC 6 cut(s) 467, 587, 950, 1478, 1565, 1657
PstI CTGCAG 1 cut(s) 1696
PsuI RGATCY 1 cut(s) 1813
PsyI GACNNNGTC 1 cut(s) 149
PvuII CAGCTG 1 cut(s) 194
RsaI GTAC 6 cut(s) 98, 161, 314, 572, 1621, 2029
RsaNI GTAC 6 cut(s) 97, 160, 313, 571, 1620, 2028
RseI CAYNNNNRTG 5 cut(s) 54, 107, 410, 425, 1644
SapI GCTCTTC 1 cut(s) 1299
SaqAI TTAA 2 cut(s) 828, 2049
Sau3AI GATC 6 cut(s) 402, 854, 1150, 1813, 1849, 1908
Sau96I GGNCC 6 cut(s) 467, 587, 950, 1478, 1565, 1657
SchI GAGTC 4 cut(s) 328, 1249, 1696, 2056
ScrFI CCNGG 9 cut(s) 282, 708, 1028, 1192, 1527, 1836, 2084, 2145, 2293
SduI GDGCHC 4 cut(s) 441, 1506, 1637, 2083
SfaNI GCATC 4 cut(s) 1111, 1309, 1394, 2045
SfcI CTRYAG 3 cut(s) 1335, 1692, 2136
SfoI GGCGCC 1 cut(s) 729
SfuI TTCGAA 1 cut(s) 138
SgrAI CRCCGGYG 2 cut(s) 422, 1450
SinI GGWCC 5 cut(s) 467, 587, 950, 1478, 1565
SmiMI CAYNNNNRTG 5 cut(s) 54, 107, 410, 425, 1644
SmlI CTYRAG 2 cut(s) 1945, 2048
SmoI CTYRAG 2 cut(s) 1945, 2048
Sse9I AATT 4 cut(s) 1410, 1483, 1891, 1975
SsiI CCGC 9 cut(s) 662, 665, 905, 908, 938, 959, 1655, 1670, 1899
SspDI GGCGCC 1 cut(s) 727
SspMI CTAG 9 cut(s) 330, 353, 717, 923, 1098, 1473, 1541, 1652, 2205
StyD4I CCNGG 9 cut(s) 280, 706, 1026, 1190, 1525, 1834, 2082, 2143, 2291
StyI CCWWGG 4 cut(s) 226, 983, 1042, 2017
TaaI ACNGT 6 cut(s) 56, 220, 883, 997, 1169, 2191
TaiI ACGT 5 cut(s) 98, 497, 515, 557, 1644
TaqI TCGA 6 cut(s) 138, 270, 524, 611, 739, 1791
TaqII GACCGA 1 cut(s) 2225
TasI AATT 4 cut(s) 1410, 1483, 1891, 1975
TauI GCSGC 5 cut(s) 665, 908, 911, 1658, 1673
TfiI GAWTC 7 cut(s) 188, 286, 608, 1108, 1174, 1391, 2165
Tru1I TTAA 2 cut(s) 828, 2049
Tru9I TTAA 2 cut(s) 828, 2049
TscAI CASTG 8 cut(s) 463, 477, 1000, 2109, 2118, 2130, 2167, 2194
TseFI GTSAC 6 cut(s) 181, 615, 1208, 1361, 1489, 2191
TseI GCWGC 8 cut(s) 277, 528, 578, 797, 1340, 1460, 1694, 1778
Tsp45I GTSAC 6 cut(s) 181, 615, 1208, 1361, 1489, 2191
TspDTI ATGAA 3 cut(s) 17, 1547, 2157
TspGWI ACGGA 1 cut(s) 654
TspRI CASTG 8 cut(s) 463, 477, 1000, 2109, 2118, 2130, 2167, 2194
Tth111I GACNNNGTC 1 cut(s) 149
Vha464I CTTAAG 1 cut(s) 2048
VpaK11BI GGWCC 5 cut(s) 467, 587, 950, 1478, 1565
XagI CCTNNNNNAGG 1 cut(s) 1664
XapI RAATTY 1 cut(s) 1483
XbaI TCTAGA 1 cut(s) 1472
XceI RCATGY 3 cut(s) 86, 202, 1811
XmiI GTMKAC 1 cut(s) 2054
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 9 cut(s) 330, 353, 717, 923, 1098, 1473, 1541, 1652, 2205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.