pycom15g06800

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001634
Physical Location & Seq
Forward (+)
4182568 .. 4184883
2316 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g06800.1

Sequence Viewer

Length: 2316 bp
ATGAAGATATCCACTTTCATGCTTCTGCTAACGGTCATGCTTCTTGCTCTCTCTCACGTCTCCGCTATTGCGGAAGACAACGCGAGAGAGCAGCAAGTGAAAAAGACTTACATCATTCACATGGACAAGTCCGAAATGCCGGCAAGTTTTGAAGATGATCGTTTCGAATGGTATGATTCGTCTTTGAAATCCGTGTCGGATTCAGCCGACATGCTTTACGCTTACAAGACCATATTCCACGGCTTCGCCACCAGGCTAACCGCCGAGGAAGCTGGGCTGCTTGAGAAGCAGTTGGGAATTCTGTCCATTCTGCCCGAAAGGAGGTACGAGCTGCATACAACTCGGACGCCCGAGTTTCTTGGATTGGAAAAAAGCGAAGCCCTCTTACCAGCGACGGATAAAGCGAGTGATTTGATAGTCGGACTGGTGGACACTGGCGTGTGGCCTGAGATCAAAAGCTACGATGACACAGGTCTCGCGGCGGTACCAAGTCGCTGGAAAGGGGTGTGTGAGGAGGGAACAAACTTCAACTCCTCGAGCTGCAACCGGAAACTCATTGGCGCGAGGTTTTTCTCTAAAGGGTATGAAGCTGAATTTGGACCAATCGATGTGAAAACAGAATCGAAATCACCACGTGATGATGATGGCCACGGAACTCACACCTCATCCACTGCAGCCGGCTCCGCAGTTTCAGGAGCTAGCCTCTTTGGTTATGCTTCGGGGACAGCGCGAGGGATGGCGCCACAGGCTCGAGTGGCCACATACAAGGCGTGCTGGCTTGGTGGGTGTTTCGGTTCTGATATCATAGCTGCAATGGAGAAGGCCATTGAAGACGGTGTCAATGTTTTATCTTTGTCTATCGGGGGACCACTGACTGATTATTACAAAGACACTGTTGCTATTGGAGCTTTCTCAGCCGTTGCACAGGGGATCCTTGTGTCGTGTTCAGCTGGTAATGGTGGGCCGGAAGCGGGGAGTTTGTCCAACGTTGCGCCTTGGATAACCACAGTCGGTGCTGGAACATTAGACAGGGATTTCCCGGCTTTTGTTAGCCTCGGAAGTGCAAAGAAATTCAGAGGTATATCGCTCTACAGGGGAACATCCTTACCCAGTGGATTACTTCCACTGGTATATGCTCGTGATGCAAGTAACTCCTCCAGTGGCGAGCTATGCGCTCCTGAAAGTTTGATTCCTGCAAAGGTTGCAGGGAAAATTGTGTTATGTGATCGAGGGGGACCGCCTAGGGTCCAAAAGAGTTTGGTGGTGAAACAGGCCGGTGGCTTAGGGATGATTTTGGCGAACACTGATGATTACGGAGAAGAACTTGTTGCGGATGCATATCTACTGCCTACAGCAGCAGTTGGTGCGATAGCTGGTGCTGCAATAAAAAGCTATATTGCCTCCGATTCTAATCCCACAGCCACAATTGCGCTTGGAGACACGGAGTTAGGTGTGCAGCCCTCGCCTGTGGTGGCATCATTCAGCTCCAGAGGACCAAATCCAGTCACTCCCGAAATACTGAAACCAGACCTAATAGCACCGGGAGTAAATATCCTTGCTGGGTGGACTGGTGCAGTTGGACCAACCGGACTGAATGAAGACAAGAGGCGGGTCAACTTCAACATCATTTCAGGTACTTCCATGTCATGCCCGCATGTGAGTGGTTTGGCCGCCCTTGTCAAGGCTGCTCGTCCAGAATGGAGCCCTGCAGCCATCAAGTCTGCACTCATGACCACATCCTACACAGCATACAAAACTGGAGAACCCATCATAGATGTAGCAACTGGAAATCCTGCAACGCCGTTTGATTATGGTGCTGGGCATGTGGATCCGGTGGCAGCCCTCGACCCTGGCCTTGTTTATGATAATGTGGTTGAGGACTACTTCAGCTTCCTCTGCGCCTTGAATTATAGCTCAAGTCAGATCAAACTAACCACTCACAAAGACTTCACTTGTGATACAAGCAAGAAATACAGCCTCGGAGAATTTAATTACCCGTCTTTTGCTGTTCCTCTAGAAACATCTTCAGGCAAAGGGGGCGGGACAGGTGCATCATCAACTGTAAAATACACAAGGACACTGACCAATGTGGGTACCCCAGGAACATACGAGGTCTCGATATCTTCGCAGGCGCCATCAGTGAAGATTTCGGTCGAACCACAATCCCTGACTTTCAGTCAAGCATACGAGAAGAAAACATATACCGTTACTTTTGTTGCAGGTTCTTCGCCATCTGGTACAAACAGCTTTGCTCAGTTGGCATGGTCCGATGGGAAACACACAGTAGGTAGTCCAATTGCTTTCAGCTGGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

772

Amino Acids

81.06

Weight (kDa)

5.42

Isoelectric Point (pI)

34.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 36 - 112 4.6e-15 Peptidase inhibitor I9
Peptidase_S8 PF00082 137 - 607 4.7e-45 Subtilase family
PA PF02225 375 - 462 1.1e-11 PA domain
fn3_6 PF17766 663 - 767 6.2e-25 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2210
Acc65I GGTACC 2 cut(s) 484, 2093
AccB1I GGYRCC 4 cut(s) 484, 739, 2093, 2131
AccII CGCG 4 cut(s) 83, 479, 563, 730
AclI AACGTT 1 cut(s) 987
AclWI GGATC 4 cut(s) 925, 938, 1823, 1836
AcoI YGGCCR 3 cut(s) 646, 756, 1668
AcsI RAATTY 4 cut(s) 297, 593, 1070, 1985
AcuI CTGAAG 2 cut(s) 1870, 2010
AcvI CACGTG 1 cut(s) 635
AcyI GRCGYC 3 cut(s) 347, 740, 2132
AdeI CACNNNGTG 1 cut(s) 635
AfaI GTAC 5 cut(s) 326, 486, 1636, 2095, 2239
AfiI CCNNNNNNNGG 3 cut(s) 321, 971, 1681
AgsI TTSAA 6 cut(s) 152, 187, 529, 830, 1621, 1906
AhdI GACNNNNNGTC 1 cut(s) 2175
AjiI CACGTC 1 cut(s) 58
AjnI CCWGG 3 cut(s) 251, 1849, 2098
AjuI GAANNNNNNNTTGG 2 cut(s) 579, 611
AleI CACNNNNGTG 1 cut(s) 437
Alw26I GTCTC 4 cut(s) 64, 479, 1431, 2119
AlwI GGATC 4 cut(s) 925, 938, 1823, 1836
Ama87I CYCGRG 3 cut(s) 350, 535, 750
AoxI GGCC 8 cut(s) 443, 646, 756, 822, 962, 1272, 1668, 1852
ApoI RAATTY 4 cut(s) 297, 593, 1070, 1985
Asp718I GGTACC 2 cut(s) 484, 2093
AspA2I CCTAGG 1 cut(s) 1241
AspLEI GCGC 8 cut(s) 563, 730, 742, 994, 1175, 1432, 1901, 2134
AspS9I GGNCC 8 cut(s) 599, 866, 962, 1235, 1246, 1493, 1580, 2265
AsuC2I CCSGG 2 cut(s) 1040, 1542
AsuHPI GGTGA 2 cut(s) 621, 1276
AsuII TTCGAA 1 cut(s) 165
AsuNHI GCTAGC 1 cut(s) 698
AvaI CYCGRG 3 cut(s) 350, 535, 750
AvaII GGWCC 7 cut(s) 599, 866, 1235, 1246, 1493, 1580, 2265
AvrII CCTAGG 1 cut(s) 1241
BalI TGGCCA 2 cut(s) 648, 758
BamHI GGATCC 2 cut(s) 930, 1828
BanI GGYRCC 4 cut(s) 484, 739, 2093, 2131
BanII GRGCYC 1 cut(s) 1706
BarI GAAGNNNNNNTAC 2 cut(s) 369, 401
BauI CACGAG 1 cut(s) 1137
BbrPI CACGTG 1 cut(s) 635
BbsI GAAGAC 3 cut(s) 81, 837, 1605
BccI CCATC 7 cut(s) 638, 730, 1721, 1775, 2143, 2239, 2264
BceAI ACGGC 3 cut(s) 256, 902, 1786
BcgI CGANNNNNNTGC 4 cut(s) 323, 357, 2208, 2242
BciT130I CCWGG 3 cut(s) 253, 1851, 2100
BcnI CCSGG 2 cut(s) 1040, 1542
BcoDI GTCTC 4 cut(s) 64, 479, 1431, 2119
BfaI CTAG 3 cut(s) 699, 1242, 2015
BfmI CTRYAG 4 cut(s) 672, 1090, 1350, 1707
BfoI RGCGCY 2 cut(s) 743, 2135
BfuAI ACCTGC 1 cut(s) 2210
BlnI CCTAGG 1 cut(s) 1241
Bme1390I CCNGG 5 cut(s) 253, 1040, 1542, 1851, 2100
Bme18I GGWCC 7 cut(s) 599, 866, 1235, 1246, 1493, 1580, 2265
BmeRI GACNNNNNGTC 1 cut(s) 2175
BmeT110I CYCGRG 3 cut(s) 350, 535, 750
BmgBI CACGTC 1 cut(s) 58
BmgT120I GGNCC 8 cut(s) 599, 866, 962, 1235, 1246, 1493, 1580, 2265
BmrFI CCNGG 5 cut(s) 253, 1040, 1542, 1851, 2100
BmrI ACTGGG 1 cut(s) 1104
BmsI GCATC 4 cut(s) 1132, 1324, 1484, 2060
BmtI GCTAGC 1 cut(s) 702
BmuI ACTGGG 1 cut(s) 1104
BoxI GACNNNNGTC 1 cut(s) 471
BpiI GAAGAC 3 cut(s) 81, 837, 1605
BplI GAGNNNNNCTC 2 cut(s) 687, 719
BpmI CTGGAG 3 cut(s) 1141, 1471, 1779
Bpu10I CCTNAGC 1 cut(s) 1282
Bpu14I TTCGAA 1 cut(s) 165
BpuEI CTTGAG 2 cut(s) 302, 1900
BpuMI CCSGG 2 cut(s) 1040, 1542
Bsa29I ATCGAT 1 cut(s) 606
BsaAI YACGTR 1 cut(s) 635
BsaBI GATNNNNATC 2 cut(s) 1338, 1410
BsaHI GRCGYC 3 cut(s) 347, 740, 2132
BsaI GGTCTC 2 cut(s) 479, 2119
BsaJI CCNNGG 9 cut(s) 238, 264, 649, 995, 1054, 1241, 1849, 1978, 2098
BsaWI WCCGGW 3 cut(s) 546, 1586, 1831
BsaXI ACNNNNNCTCC 2 cut(s) 515, 545
Bsc4I CCNNNNNNNGG 3 cut(s) 321, 971, 1681
Bse118I RCCGGY 3 cut(s) 139, 677, 1274
Bse1I ACTGG 9 cut(s) 429, 439, 1110, 1131, 1158, 1502, 1573, 1762, 1789
Bse3DI GCAATG 1 cut(s) 819
Bse8I GATNNNNATC 2 cut(s) 1338, 1410
BseBI CCWGG 3 cut(s) 253, 1851, 2100
BseCI ATCGAT 1 cut(s) 606
BseDI CCNNGG 9 cut(s) 238, 264, 649, 995, 1054, 1241, 1849, 1978, 2098
BseGI GGATG 6 cut(s) 665, 741, 1100, 1293, 1339, 1736
BseJI GATNNNNATC 2 cut(s) 1338, 1410
BseLI CCNNNNNNNGG 3 cut(s) 321, 971, 1681
BseMI GCAATG 1 cut(s) 819
BseMII CTCAG 3 cut(s) 438, 927, 2267
BseNI ACTGG 9 cut(s) 429, 439, 1110, 1131, 1158, 1502, 1573, 1762, 1789
BseRI GAGGAG 3 cut(s) 523, 527, 1144
BseYI CCCAGC 3 cut(s) 272, 1559, 1817
BsgI GTGCAG 3 cut(s) 1475, 1593, 1707
Bsh1236I CGCG 4 cut(s) 83, 479, 563, 730
Bsh1285I CGRYCG 1 cut(s) 2154
BshFI GGCC 8 cut(s) 445, 648, 758, 824, 964, 1274, 1670, 1854
BshNI GGYRCC 4 cut(s) 484, 739, 2093, 2131
BshVI ATCGAT 1 cut(s) 606
BsiEI CGRYCG 1 cut(s) 2154
BsiHKCI CYCGRG 3 cut(s) 350, 535, 750
BsiSI CCGG 9 cut(s) 140, 547, 678, 965, 1040, 1275, 1541, 1587, 1832
BslFI GGGAC 4 cut(s) 736, 879, 1248, 2056
BslI CCNNNNNNNGG 3 cut(s) 321, 971, 1681
BsmAI GTCTC 4 cut(s) 64, 479, 1431, 2119
BsmBI CGTCTC 1 cut(s) 64
BsmFI GGGAC 4 cut(s) 736, 879, 1248, 2056
BsnI GGCC 8 cut(s) 445, 648, 758, 824, 964, 1274, 1670, 1854
Bso31I GGTCTC 2 cut(s) 479, 2119
BsoBI CYCGRG 3 cut(s) 350, 535, 750
Bsp119I TTCGAA 1 cut(s) 165
Bsp1286I GDGCHC 1 cut(s) 1706
Bsp143I GATC 6 cut(s) 157, 450, 930, 1225, 1828, 1923
BspANI GGCC 8 cut(s) 445, 648, 758, 824, 964, 1274, 1670, 1854
BspCNI CTCAG 3 cut(s) 439, 926, 2266
BspDI ATCGAT 1 cut(s) 606
BspFNI CGCG 4 cut(s) 83, 479, 563, 730
BspHI TCATGA 1 cut(s) 1728
BspMAI CTGCAG 2 cut(s) 676, 1711
BspMI ACCTGC 1 cut(s) 2210
BspOI GCTAGC 1 cut(s) 702
BspPI GGATC 4 cut(s) 925, 938, 1823, 1836
BspT104I TTCGAA 1 cut(s) 165
BspT107I GGYRCC 4 cut(s) 484, 739, 2093, 2131
BspTNI GGTCTC 2 cut(s) 479, 2119
BsrDI GCAATG 1 cut(s) 819
BsrFI RCCGGY 3 cut(s) 139, 677, 1274
BsrI ACTGG 9 cut(s) 429, 439, 1110, 1131, 1158, 1502, 1573, 1762, 1789
BssAI RCCGGY 3 cut(s) 139, 677, 1274
BssECI CCNNGG 9 cut(s) 238, 264, 649, 995, 1054, 1241, 1849, 1978, 2098
BssMI GATC 6 cut(s) 157, 450, 930, 1225, 1828, 1923
BssNI GRCGYC 3 cut(s) 347, 740, 2132
BssSI CACGAG 1 cut(s) 1137
BssT1I CCWWGG 2 cut(s) 995, 1241
Bst2BI CACGAG 1 cut(s) 1137
Bst2UI CCWGG 3 cut(s) 253, 1851, 2100
Bst4CI ACNGT 7 cut(s) 34, 836, 895, 1009, 2062, 2206, 2284
BstACI GRCGYC 3 cut(s) 347, 740, 2132
BstAPI GCANNNNNTGC 2 cut(s) 1202, 1364
BstBAI YACGTR 1 cut(s) 635
BstBI TTCGAA 1 cut(s) 165
BstC8I GCNNGC 8 cut(s) 141, 679, 700, 772, 776, 1166, 1652, 2130
BstDEI CTNAG 4 cut(s) 447, 913, 1282, 2253
BstDSI CCRYGG 2 cut(s) 238, 649
BstENI CCTNNNNNAGG 1 cut(s) 1679
BstF5I GGATG 6 cut(s) 665, 741, 1100, 1293, 1339, 1736
BstFNI CGCG 4 cut(s) 83, 479, 563, 730
BstH2I RGCGCY 2 cut(s) 743, 2135
BstHHI GCGC 8 cut(s) 563, 730, 742, 994, 1175, 1432, 1901, 2134
BstKTI GATC 6 cut(s) 160, 453, 933, 1228, 1831, 1926
BstMAI GTCTC 4 cut(s) 64, 479, 1431, 2119
BstMBI GATC 6 cut(s) 157, 450, 930, 1225, 1828, 1923
BstMCI CGRYCG 1 cut(s) 2154
BstNI CCWGG 3 cut(s) 253, 1851, 2100
BstNSI RCATGY 3 cut(s) 214, 1658, 1826
BstPAI GACNNNNGTC 1 cut(s) 471
BstSCI CCNGG 5 cut(s) 251, 1038, 1540, 1849, 2098
BstSFI CTRYAG 4 cut(s) 672, 1090, 1350, 1707
BstUI CGCG 4 cut(s) 83, 479, 563, 730
BstV2I GAAGAC 3 cut(s) 81, 837, 1605
BstX2I RGATCY 2 cut(s) 930, 1828
BstXI CCANNNNNNTGG 1 cut(s) 495
BstYI RGATCY 2 cut(s) 930, 1828
Bsu15I ATCGAT 1 cut(s) 606
BsuRI GGCC 8 cut(s) 445, 648, 758, 824, 964, 1274, 1670, 1854
BsuTUI ATCGAT 1 cut(s) 606
BtgI CCRYGG 2 cut(s) 238, 649
BtrI CACGTC 1 cut(s) 58
BtsCI GGATG 6 cut(s) 665, 741, 1100, 1293, 1339, 1736
BtsI GCAGTG 1 cut(s) 669
BveI ACCTGC 1 cut(s) 2210
Cac8I GCNNGC 8 cut(s) 141, 679, 700, 772, 776, 1166, 1652, 2130
CciI TCATGA 1 cut(s) 1728
CfoI GCGC 8 cut(s) 563, 730, 742, 994, 1175, 1432, 1901, 2134
Cfr10I RCCGGY 3 cut(s) 139, 677, 1274
Cfr13I GGNCC 8 cut(s) 599, 866, 962, 1235, 1246, 1493, 1580, 2265
ClaI ATCGAT 1 cut(s) 606
CseI GACGC 1 cut(s) 355
Csp6I GTAC 5 cut(s) 325, 485, 1635, 2094, 2238
CviQI GTAC 5 cut(s) 325, 485, 1635, 2094, 2238
DdeI CTNAG 4 cut(s) 447, 913, 1282, 2253
DinI GGCGCC 2 cut(s) 741, 2133
DpnI GATC 6 cut(s) 159, 452, 932, 1227, 1830, 1925
DpnII GATC 6 cut(s) 157, 450, 930, 1225, 1828, 1923
DraIII CACNNNGTG 1 cut(s) 635
DriI GACNNNNNGTC 1 cut(s) 2175
EaeI YGGCCR 3 cut(s) 646, 756, 1668
Eam1105I GACNNNNNGTC 1 cut(s) 2175
Eco130I CCWWGG 2 cut(s) 995, 1241
Eco24I GRGCYC 1 cut(s) 1706
Eco31I GGTCTC 2 cut(s) 479, 2119
Eco32I GATATC 3 cut(s) 9, 802, 2121
Eco47I GGWCC 7 cut(s) 599, 866, 1235, 1246, 1493, 1580, 2265
Eco57I CTGAAG 2 cut(s) 1870, 2010
Eco72I CACGTG 1 cut(s) 635
Eco88I CYCGRG 3 cut(s) 350, 535, 750
EcoNI CCTNNNNNAGG 1 cut(s) 1679
EcoRI GAATTC 1 cut(s) 297
EcoRII CCWGG 3 cut(s) 251, 1849, 2098
EcoRV GATATC 3 cut(s) 9, 802, 2121
EcoT14I CCWWGG 2 cut(s) 995, 1241
EcoT22I ATGCAT 1 cut(s) 1339
EcoT38I GRGCYC 1 cut(s) 1706
EgeI GGCGCC 2 cut(s) 741, 2133
EheI GGCGCC 2 cut(s) 741, 2133
ErhI CCWWGG 2 cut(s) 995, 1241
Esp3I CGTCTC 1 cut(s) 64
FaqI GGGAC 4 cut(s) 736, 879, 1248, 2056
FauI CCCGC 4 cut(s) 964, 1602, 1659, 2033
FokI GGATG 6 cut(s) 652, 748, 1087, 1300, 1346, 1723
FriOI GRGCYC 1 cut(s) 1706
FspBI CTAG 3 cut(s) 699, 1242, 2015
GlaI GCGC 8 cut(s) 562, 729, 741, 993, 1174, 1431, 1900, 2133
GsaI CCCAGC 3 cut(s) 276, 1563, 1821
GsuI CTGGAG 3 cut(s) 1141, 1471, 1779
HaeII RGCGCY 2 cut(s) 743, 2135
HaeIII GGCC 8 cut(s) 445, 648, 758, 824, 964, 1274, 1670, 1854
HapII CCGG 9 cut(s) 140, 547, 678, 965, 1040, 1275, 1541, 1587, 1832
HgaI GACGC 1 cut(s) 355
HhaI GCGC 8 cut(s) 563, 730, 742, 994, 1175, 1432, 1901, 2134
Hin1I GRCGYC 3 cut(s) 347, 740, 2132
Hin6I GCGC 8 cut(s) 561, 728, 740, 992, 1173, 1430, 1899, 2132
HinP1I GCGC 8 cut(s) 561, 728, 740, 992, 1173, 1430, 1899, 2132
HincII GTYRAC 1 cut(s) 1615
HindII GTYRAC 1 cut(s) 1615
HinfI GANTC 5 cut(s) 176, 200, 620, 1189, 1406
HpaII CCGG 9 cut(s) 140, 547, 678, 965, 1040, 1275, 1541, 1587, 1832
HphI GGTGA 2 cut(s) 621, 1276
Hpy166II GTNNAC 3 cut(s) 430, 1566, 1615
Hpy188III TCNNGA 9 cut(s) 693, 1139, 1178, 1488, 1511, 1694, 1729, 2015, 2116
Hpy8I GTNNAC 3 cut(s) 430, 1566, 1615
Hpy99I CGWCG 1 cut(s) 397
HpyAV CCTTC 1 cut(s) 814
HpyCH4III ACNGT 7 cut(s) 34, 836, 895, 1009, 2062, 2206, 2284
HpyCH4IV ACGT 3 cut(s) 57, 634, 987
HpyF3I CTNAG 4 cut(s) 447, 913, 1282, 2253
HpySE526I ACGT 3 cut(s) 57, 634, 987
Hsp92I GRCGYC 3 cut(s) 347, 740, 2132
HspAI GCGC 8 cut(s) 561, 728, 740, 992, 1173, 1430, 1899, 2132
KasI GGCGCC 2 cut(s) 739, 2131
KpnI GGTACC 2 cut(s) 488, 2097
KroI GCCGGC 2 cut(s) 139, 677
KroNI GCCGGC 2 cut(s) 141, 679
Kzo9I GATC 6 cut(s) 157, 450, 930, 1225, 1828, 1923
LmnI GCTCC 6 cut(s) 686, 695, 905, 1180, 1490, 1701
LweI GCATC 4 cut(s) 1132, 1324, 1484, 2060
MaeI CTAG 3 cut(s) 699, 1242, 2015
MaeII ACGT 3 cut(s) 57, 634, 987
MaeIII GTNAC 3 cut(s) 1148, 1504, 2206
MalI GATC 6 cut(s) 159, 452, 932, 1227, 1830, 1925
MboI GATC 6 cut(s) 157, 450, 930, 1225, 1828, 1923
MfeI CAATTG 2 cut(s) 1425, 2295
MflI RGATCY 2 cut(s) 930, 1828
MhlI GDGCHC 1 cut(s) 1706
MlsI TGGCCA 2 cut(s) 648, 758
MluCI AATT 9 cut(s) 297, 593, 1070, 1212, 1425, 1906, 1985, 1990, 2295
MluNI TGGCCA 2 cut(s) 648, 758
Mly113I GGCGCC 2 cut(s) 740, 2132
MmeI TCCRAC 4 cut(s) 177, 400, 1008, 1558
Mox20I TGGCCA 2 cut(s) 648, 758
Mph1103I ATGCAT 1 cut(s) 1339
MroNI GCCGGC 2 cut(s) 139, 677
MscI TGGCCA 2 cut(s) 648, 758
MseI TTAA 1 cut(s) 1989
MslI CAYNNNNRTG 4 cut(s) 17, 119, 437, 1659
Msp20I TGGCCA 2 cut(s) 648, 758
MspA1I CMGCKG 2 cut(s) 950, 2307
MspI CCGG 9 cut(s) 140, 547, 678, 965, 1040, 1275, 1541, 1587, 1832
MspR9I CCNGG 5 cut(s) 253, 1040, 1542, 1851, 2100
MunI CAATTG 2 cut(s) 1425, 2295
MvaI CCWGG 3 cut(s) 253, 1851, 2100
MvnI CGCG 4 cut(s) 83, 479, 563, 730
NaeI GCCGGC 2 cut(s) 141, 679
NarI GGCGCC 2 cut(s) 740, 2132
NciI CCSGG 2 cut(s) 1040, 1542
NdeII GATC 6 cut(s) 157, 450, 930, 1225, 1828, 1923
NgoMIV GCCGGC 2 cut(s) 139, 677
NheI GCTAGC 1 cut(s) 698
NmeAIII GCCGAG 1 cut(s) 289
NmuCI GTSAC 1 cut(s) 1504
NsiI ATGCAT 1 cut(s) 1339
NspI RCATGY 3 cut(s) 214, 1658, 1826
NspV TTCGAA 1 cut(s) 165
OliI CACNNNNGTG 1 cut(s) 437
PaeR7I CTCGAG 2 cut(s) 535, 750
PagI TCATGA 1 cut(s) 1728
PcsI WCGNNNNNNNCGW 1 cut(s) 401
PdiI GCCGGC 2 cut(s) 141, 679
PfeI GAWTC 5 cut(s) 176, 200, 620, 1189, 1406
PflFI GACNNNGTC 1 cut(s) 836
PluTI GGCGCC 2 cut(s) 743, 2135
PmaCI CACGTG 1 cut(s) 635
PmlI CACGTG 1 cut(s) 635
Ppu21I YACGTR 1 cut(s) 635
PshAI GACNNNNGTC 1 cut(s) 471
Psp1406I AACGTT 1 cut(s) 987
Psp6I CCWGG 3 cut(s) 251, 1849, 2098
PspCI CACGTG 1 cut(s) 635
PspFI CCCAGC 3 cut(s) 272, 1559, 1817
PspGI CCWGG 3 cut(s) 251, 1849, 2098
PspPI GGNCC 8 cut(s) 599, 866, 962, 1235, 1246, 1493, 1580, 2265
PspXI VCTCGAGB 2 cut(s) 535, 750
PstI CTGCAG 2 cut(s) 676, 1711
PsuI RGATCY 2 cut(s) 930, 1828
PsyI GACNNNGTC 1 cut(s) 836
PvuII CAGCTG 2 cut(s) 950, 2307
RsaI GTAC 5 cut(s) 326, 486, 1636, 2095, 2239
RsaNI GTAC 5 cut(s) 325, 485, 1635, 2094, 2238
RseI CAYNNNNRTG 4 cut(s) 17, 119, 437, 1659
SaqAI TTAA 1 cut(s) 1989
Sau3AI GATC 6 cut(s) 157, 450, 930, 1225, 1828, 1923
Sau96I GGNCC 8 cut(s) 599, 866, 962, 1235, 1246, 1493, 1580, 2265
ScrFI CCNGG 5 cut(s) 253, 1040, 1542, 1851, 2100
SduI GDGCHC 1 cut(s) 1706
SfaNI GCATC 4 cut(s) 1132, 1324, 1484, 2060
SfcI CTRYAG 4 cut(s) 672, 1090, 1350, 1707
SfoI GGCGCC 2 cut(s) 741, 2133
Sfr274I CTCGAG 2 cut(s) 535, 750
SfuI TTCGAA 1 cut(s) 165
SinI GGWCC 7 cut(s) 599, 866, 1235, 1246, 1493, 1580, 2265
SlaI CTCGAG 2 cut(s) 535, 750
SmiMI CAYNNNNRTG 4 cut(s) 17, 119, 437, 1659
SmlI CTYRAG 4 cut(s) 281, 535, 750, 1915
SmoI CTYRAG 4 cut(s) 281, 535, 750, 1915
Sse9I AATT 9 cut(s) 297, 593, 1070, 1212, 1425, 1906, 1985, 1990, 2295
SspDI GGCGCC 2 cut(s) 739, 2131
SspMI CTAG 3 cut(s) 699, 1242, 2015
StyD4I CCNGG 5 cut(s) 251, 1038, 1540, 1849, 2098
StyI CCWWGG 2 cut(s) 995, 1241
TaaI ACNGT 7 cut(s) 34, 836, 895, 1009, 2062, 2206, 2284
TaiI ACGT 3 cut(s) 60, 637, 990
TaqI TCGA 9 cut(s) 165, 536, 606, 623, 751, 1228, 1845, 2117, 2154
TaqII GACCGA 1 cut(s) 2140
TasI AATT 9 cut(s) 297, 593, 1070, 1212, 1425, 1906, 1985, 1990, 2295
TauI GCSGC 2 cut(s) 482, 1673
TfiI GAWTC 5 cut(s) 176, 200, 620, 1189, 1406
Tru1I TTAA 1 cut(s) 1989
Tru9I TTAA 1 cut(s) 1989
TseFI GTSAC 1 cut(s) 1504
Tsp45I GTSAC 1 cut(s) 1504
TspDTI ATGAA 4 cut(s) 7, 17, 600, 1611
TspGWI ACGGA 5 cut(s) 181, 410, 666, 1329, 1457
Tth111I GACNNNGTC 1 cut(s) 836
VpaK11BI GGWCC 7 cut(s) 599, 866, 1235, 1246, 1493, 1580, 2265
XagI CCTNNNNNAGG 1 cut(s) 1679
XapI RAATTY 4 cut(s) 297, 593, 1070, 1985
XbaI TCTAGA 1 cut(s) 2014
XceI RCATGY 3 cut(s) 214, 1658, 1826
XhoI CTCGAG 2 cut(s) 535, 750
XmaJI CCTAGG 1 cut(s) 1241
XspI CTAG 3 cut(s) 699, 1242, 2015
Zsp2I ATGCAT 1 cut(s) 1339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.