subtilisin-like protease
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 158 |
| AccB1I | GGYRCC | 3 cut(s) | 736, 2087, 2579 |
| AccBSI | CCGCTC | 3 cut(s) | 3270, 3296, 3422 |
| AccI | GTMKAC | 2 cut(s) | 1854, 2445 |
| AccII | CGCG | 4 cut(s) | 140, 917, 1664, 3066 |
| AccIII | TCCGGA | 1 cut(s) | 346 |
| AclWI | GGATC | 5 cut(s) | 871, 1817, 1830, 2694, 2707 |
| AcoI | YGGCCR | 6 cut(s) | 440, 643, 753, 1357, 1704, 2665 |
| AcsI | RAATTY | 5 cut(s) | 294, 1067, 1492, 1978, 2331 |
| AcuI | CTGAAG | 3 cut(s) | 1611, 2004, 3225 |
| AcvI | CACGTG | 2 cut(s) | 1651, 3430 |
| AcyI | GRCGYC | 3 cut(s) | 737, 1446, 2580 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 438, 968, 1675, 2711, 3289 |
| AflIII | ACRYGT | 2 cut(s) | 216, 1648 |
| AgeI | ACCGGT | 1 cut(s) | 1459 |
| AgsI | TTSAA | 9 cut(s) | 526, 1244, 1394, 1615, 1900, 1984, 2318, 2548, 3137 |
| AjnI | CCWGG | 6 cut(s) | 289, 715, 1534, 1843, 2582, 2710 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 576, 608, 2598, 2630 |
| AleI | CACNNNNGTG | 3 cut(s) | 63, 434, 1653 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2691, 2723 |
| Alw21I | GWGCWC | 2 cut(s) | 450, 2896 |
| Alw26I | GTCTC | 3 cut(s) | 196, 879, 2113 |
| AlwI | GGATC | 5 cut(s) | 871, 1817, 1830, 2694, 2707 |
| Ama87I | CYCGRG | 1 cut(s) | 747 |
| Aor13HI | TCCGGA | 1 cut(s) | 346 |
| ApoI | RAATTY | 5 cut(s) | 294, 1067, 1492, 1978, 2331 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 455, 487 |
| AsiGI | ACCGGT | 1 cut(s) | 1459 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1940 |
| AspLEI | GCGC | 6 cut(s) | 739, 922, 991, 1013, 1568, 2582 |
| AspS9I | GGNCC | 8 cut(s) | 476, 596, 959, 1487, 1574, 1666, 2618, 2933 |
| AsuC2I | CCSGG | 6 cut(s) | 1037, 1173, 1201, 2093, 2889, 3002 |
| AsuNHI | GCTAGC | 3 cut(s) | 695, 2213, 2717 |
| AvaI | CYCGRG | 1 cut(s) | 747 |
| AvaII | GGWCC | 6 cut(s) | 476, 596, 959, 1487, 1574, 2618 |
| BaeGI | GKGCMC | 2 cut(s) | 2092, 3429 |
| BalI | TGGCCA | 4 cut(s) | 645, 755, 1359, 2667 |
| BamHI | GGATCC | 2 cut(s) | 1822, 2699 |
| BanI | GGYRCC | 3 cut(s) | 736, 2087, 2579 |
| BanII | GRGCYC | 4 cut(s) | 450, 1662, 1700, 3314 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 366, 398, 2177, 2209 |
| BauI | CACGAG | 2 cut(s) | 2652, 2769 |
| BbrPI | CACGTG | 2 cut(s) | 1651, 3430 |
| BbsI | GAAGAC | 4 cut(s) | 1599, 1603, 2195, 3213 |
| Bbv12I | GWGCWC | 2 cut(s) | 450, 2896 |
| BccI | CCATC | 9 cut(s) | 394, 635, 824, 2233, 2258, 2630, 2657, 2846, 2924 |
| BceAI | ACGGC | 2 cut(s) | 187, 1113 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 4, 38 |
| BciT130I | CCWGG | 6 cut(s) | 291, 717, 1536, 1845, 2584, 2712 |
| BclI | TGATCA | 1 cut(s) | 411 |
| BcnI | CCSGG | 6 cut(s) | 1037, 1173, 1201, 2093, 2889, 3002 |
| BcoDI | GTCTC | 3 cut(s) | 196, 879, 2113 |
| BfmI | CTRYAG | 1 cut(s) | 1344 |
| BfoI | RGCGCY | 3 cut(s) | 740, 1014, 2583 |
| BlpI | GCTNAGC | 3 cut(s) | 270, 2307, 3221 |
| BmcAI | AGTACT | 1 cut(s) | 1512 |
| Bme18I | GGWCC | 6 cut(s) | 476, 596, 959, 1487, 1574, 2618 |
| BmeT110I | CYCGRG | 1 cut(s) | 747 |
| BmgT120I | GGNCC | 8 cut(s) | 476, 596, 959, 1487, 1574, 1666, 2618, 2933 |
| BmiI | GGNNCC | 8 cut(s) | 738, 1697, 1824, 2089, 2581, 2620, 2701, 3311 |
| BmsI | GCATC | 6 cut(s) | 20, 1120, 1318, 1363, 2054, 2164 |
| BmtI | GCTAGC | 3 cut(s) | 699, 2217, 2721 |
| BoxI | GACNNNNGTC | 1 cut(s) | 2487 |
| BpiI | GAAGAC | 4 cut(s) | 1599, 1603, 2195, 3213 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 2706, 2738, 2894, 2926 |
| BpmI | CTGGAG | 5 cut(s) | 699, 1488, 1714, 1773, 2668 |
| Bpu1102I | GCTNAGC | 3 cut(s) | 270, 2307, 3221 |
| BpuEI | CTTGAG | 3 cut(s) | 1939, 2539, 3272 |
| BpuMI | CCSGG | 6 cut(s) | 1037, 1173, 1201, 2093, 2889, 3002 |
| BsaAI | YACGTR | 3 cut(s) | 219, 1651, 3430 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1404 |
| BsaHI | GRCGYC | 3 cut(s) | 737, 1446, 2580 |
| BsaI | GGTCTC | 2 cut(s) | 196, 2113 |
| BsaWI | WCCGGW | 6 cut(s) | 346, 961, 1459, 2228, 2383, 2921 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 328, 358, 2534, 2564 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 438, 968, 1675, 2711, 3289 |
| Bse118I | RCCGGY | 3 cut(s) | 385, 431, 1459 |
| Bse3DI | GCAATG | 6 cut(s) | 816, 1144, 2485, 2688, 2838, 3185 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1404 |
| BseAI | TCCGGA | 1 cut(s) | 346 |
| BseBI | CCWGG | 6 cut(s) | 291, 717, 1536, 1845, 2584, 2712 |
| BseGI | GGATG | 9 cut(s) | 231, 835, 1545, 1631, 2225, 2951, 3123, 3245, 3344 |
| BseJI | GATNNNNATC | 1 cut(s) | 1404 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 438, 968, 1675, 2711, 3289 |
| BseMI | GCAATG | 6 cut(s) | 816, 1144, 2485, 2688, 2838, 3185 |
| BseMII | CTCAG | 7 cut(s) | 261, 1928, 2153, 2298, 2340, 2390, 2454 |
| BseRI | GAGGAG | 3 cut(s) | 1615, 2542, 2546 |
| BseSI | GKGCMC | 2 cut(s) | 2092, 3429 |
| BseYI | CCCAGC | 3 cut(s) | 1553, 1811, 3010 |
| BsgI | GTGCAG | 2 cut(s) | 1186, 2745 |
| Bsh1236I | CGCG | 4 cut(s) | 140, 917, 1664, 3066 |
| BshNI | GGYRCC | 3 cut(s) | 736, 2087, 2579 |
| BshTI | ACCGGT | 1 cut(s) | 1459 |
| BsiHKAI | GWGCWC | 2 cut(s) | 450, 2896 |
| BsiHKCI | CYCGRG | 1 cut(s) | 747 |
| BslFI | GGGAC | 5 cut(s) | 112, 1043, 1155, 2755, 3017 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 438, 968, 1675, 2711, 3289 |
| BsmAI | GTCTC | 3 cut(s) | 196, 879, 2113 |
| BsmFI | GGGAC | 5 cut(s) | 112, 1043, 1155, 2755, 3017 |
| BsmI | GAATGC | 1 cut(s) | 727 |
| Bso31I | GGTCTC | 2 cut(s) | 196, 2113 |
| BsoBI | CYCGRG | 1 cut(s) | 747 |
| Bsp1286I | GDGCHC | 7 cut(s) | 450, 1662, 1700, 2092, 2896, 3314, 3429 |
| Bsp13I | TCCGGA | 1 cut(s) | 346 |
| Bsp143I | GATC | 9 cut(s) | 411, 863, 1159, 1822, 1858, 1917, 2129, 2645, 2699 |
| Bsp1720I | GCTNAGC | 3 cut(s) | 270, 2307, 3221 |
| Bsp19I | CCATGG | 3 cut(s) | 235, 646, 2668 |
| BspCNI | CTCAG | 7 cut(s) | 262, 1927, 2154, 2299, 2341, 2389, 2455 |
| BspEI | TCCGGA | 1 cut(s) | 346 |
| BspFNI | CGCG | 4 cut(s) | 140, 917, 1664, 3066 |
| BspHI | TCATGA | 2 cut(s) | 1722, 3336 |
| BspLI | GGNNCC | 8 cut(s) | 738, 1697, 1824, 2089, 2581, 2620, 2701, 3311 |
| BspOI | GCTAGC | 3 cut(s) | 699, 2217, 2721 |
| BspPI | GGATC | 5 cut(s) | 871, 1817, 1830, 2694, 2707 |
| BspQI | GCTCTTC | 1 cut(s) | 1308 |
| BspT107I | GGYRCC | 3 cut(s) | 736, 2087, 2579 |
| BspTNI | GGTCTC | 2 cut(s) | 196, 2113 |
| BsrBI | CCGCTC | 3 cut(s) | 3270, 3296, 3422 |
| BsrDI | GCAATG | 6 cut(s) | 816, 1144, 2485, 2688, 2838, 3185 |
| BsrFI | RCCGGY | 3 cut(s) | 385, 431, 1459 |
| BssAI | RCCGGY | 3 cut(s) | 385, 431, 1459 |
| BssMI | GATC | 9 cut(s) | 411, 863, 1159, 1822, 1858, 1917, 2129, 2645, 2699 |
| BssNI | GRCGYC | 3 cut(s) | 737, 1446, 2580 |
| BssSI | CACGAG | 2 cut(s) | 2652, 2769 |
| BssT1I | CCWWGG | 8 cut(s) | 235, 646, 703, 992, 1051, 2026, 2504, 2668 |
| Bst2BI | CACGAG | 2 cut(s) | 2652, 2769 |
| Bst2UI | CCWGG | 6 cut(s) | 291, 717, 1536, 1845, 2584, 2712 |
| Bst4CI | ACNGT | 7 cut(s) | 65, 229, 892, 1006, 2200, 2980, 3437 |
| Bst6I | CTCTTC | 3 cut(s) | 75, 535, 1308 |
| BstACI | GRCGYC | 3 cut(s) | 737, 1446, 2580 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1196, 2475 |
| BstBAI | YACGTR | 3 cut(s) | 219, 1651, 3430 |
| BstDSI | CCRYGG | 5 cut(s) | 235, 646, 915, 2668, 3064 |
| BstENI | CCTNNNNNAGG | 3 cut(s) | 1673, 2709, 3287 |
| BstF5I | GGATG | 9 cut(s) | 231, 835, 1545, 1631, 2225, 2951, 3123, 3245, 3344 |
| BstFNI | CGCG | 4 cut(s) | 140, 917, 1664, 3066 |
| BstH2I | RGCGCY | 3 cut(s) | 740, 1014, 2583 |
| BstHHI | GCGC | 6 cut(s) | 739, 922, 991, 1013, 1568, 2582 |
| BstKTI | GATC | 9 cut(s) | 414, 866, 1162, 1825, 1861, 1920, 2132, 2648, 2702 |
| BstMAI | GTCTC | 3 cut(s) | 196, 879, 2113 |
| BstMBI | GATC | 9 cut(s) | 411, 863, 1159, 1822, 1858, 1917, 2129, 2645, 2699 |
| BstNI | CCWGG | 6 cut(s) | 291, 717, 1536, 1845, 2584, 2712 |
| BstNSI | RCATGY | 4 cut(s) | 95, 211, 1820, 2793 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 2487 |
| BstSFI | CTRYAG | 1 cut(s) | 1344 |
| BstSLI | GKGCMC | 2 cut(s) | 2092, 3429 |
| BstUI | CGCG | 4 cut(s) | 140, 917, 1664, 3066 |
| BstV2I | GAAGAC | 4 cut(s) | 1599, 1603, 2195, 3213 |
| BstX2I | RGATCY | 2 cut(s) | 1822, 2699 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 1367, 2033, 2511 |
| BstYI | RGATCY | 2 cut(s) | 1822, 2699 |
| BtgI | CCRYGG | 5 cut(s) | 235, 646, 915, 2668, 3064 |
| BtsCI | GGATG | 9 cut(s) | 231, 835, 1545, 1631, 2225, 2951, 3123, 3245, 3344 |
| BtsI | GCAGTG | 1 cut(s) | 2528 |
| CciI | TCATGA | 2 cut(s) | 1722, 3336 |
| CfoI | GCGC | 6 cut(s) | 739, 922, 991, 1013, 1568, 2582 |
| Cfr10I | RCCGGY | 3 cut(s) | 385, 431, 1459 |
| Cfr13I | GGNCC | 8 cut(s) | 476, 596, 959, 1487, 1574, 1666, 2618, 2933 |
| Cfr42I | CCGCGG | 2 cut(s) | 918, 3067 |
| CseI | GACGC | 2 cut(s) | 1435, 2724 |
| CspAI | ACCGGT | 1 cut(s) | 1459 |
| DinI | GGCGCC | 2 cut(s) | 738, 2581 |
| DpnI | GATC | 9 cut(s) | 413, 865, 1161, 1824, 1860, 1919, 2131, 2647, 2701 |
| DpnII | GATC | 9 cut(s) | 411, 863, 1159, 1822, 1858, 1917, 2129, 2645, 2699 |
| DraI | TTTAAA | 1 cut(s) | 2058 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 158 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 158 |
| EaeI | YGGCCR | 6 cut(s) | 440, 643, 753, 1357, 1704, 2665 |
| Eam1104I | CTCTTC | 3 cut(s) | 75, 535, 1308 |
| EarI | CTCTTC | 3 cut(s) | 75, 535, 1308 |
| Ecl136II | GAGCTC | 1 cut(s) | 448 |
| Eco130I | CCWWGG | 8 cut(s) | 235, 646, 703, 992, 1051, 2026, 2504, 2668 |
| Eco24I | GRGCYC | 4 cut(s) | 450, 1662, 1700, 3314 |
| Eco31I | GGTCTC | 2 cut(s) | 196, 2113 |
| Eco32I | GATATC | 1 cut(s) | 799 |
| Eco47I | GGWCC | 6 cut(s) | 476, 596, 959, 1487, 1574, 2618 |
| Eco53kI | GAGCTC | 1 cut(s) | 448 |
| Eco57I | CTGAAG | 3 cut(s) | 1611, 2004, 3225 |
| Eco72I | CACGTG | 2 cut(s) | 1651, 3430 |
| Eco88I | CYCGRG | 1 cut(s) | 747 |
| EcoICRI | GAGCTC | 1 cut(s) | 448 |
| EcoNI | CCTNNNNNAGG | 3 cut(s) | 1673, 2709, 3287 |
| EcoRI | GAATTC | 2 cut(s) | 294, 2331 |
| EcoRII | CCWGG | 6 cut(s) | 289, 715, 1534, 1843, 2582, 2710 |
| EcoRV | GATATC | 1 cut(s) | 799 |
| EcoT14I | CCWWGG | 8 cut(s) | 235, 646, 703, 992, 1051, 2026, 2504, 2668 |
| EcoT22I | ATGCAT | 1 cut(s) | 1333 |
| EcoT38I | GRGCYC | 4 cut(s) | 450, 1662, 1700, 3314 |
| EgeI | GGCGCC | 2 cut(s) | 738, 2581 |
| EheI | GGCGCC | 2 cut(s) | 738, 2581 |
| ErhI | CCWWGG | 8 cut(s) | 235, 646, 703, 992, 1051, 2026, 2504, 2668 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2598, 2630 |
| FaqI | GGGAC | 5 cut(s) | 112, 1043, 1155, 2755, 3017 |
| FauI | CCCGC | 5 cut(s) | 145, 667, 961, 3263, 3415 |
| FauNDI | CATATG | 1 cut(s) | 1306 |
| FbaI | TGATCA | 1 cut(s) | 411 |
| FblI | GTMKAC | 2 cut(s) | 1854, 2445 |
| FokI | GGATG | 9 cut(s) | 218, 842, 1532, 1618, 2212, 2938, 3130, 3232, 3331 |
| FriOI | GRGCYC | 4 cut(s) | 450, 1662, 1700, 3314 |
| GlaI | GCGC | 6 cut(s) | 738, 921, 990, 1012, 1567, 2581 |
| GsaI | CCCAGC | 3 cut(s) | 1557, 1815, 3014 |
| GsuI | CTGGAG | 5 cut(s) | 699, 1488, 1714, 1773, 2668 |
| HaeII | RGCGCY | 3 cut(s) | 740, 1014, 2583 |
| HgaI | GACGC | 2 cut(s) | 1435, 2724 |
| HhaI | GCGC | 6 cut(s) | 739, 922, 991, 1013, 1568, 2582 |
| Hin1I | GRCGYC | 3 cut(s) | 737, 1446, 2580 |
| Hin6I | GCGC | 6 cut(s) | 737, 920, 989, 1011, 1566, 2580 |
| HinP1I | GCGC | 6 cut(s) | 737, 920, 989, 1011, 1566, 2580 |
| HincII | GTYRAC | 2 cut(s) | 163, 2860 |
| HindII | GTYRAC | 2 cut(s) | 163, 2860 |
| HindIII | AAGCTT | 1 cut(s) | 973 |
| Hpy99I | CGWCG | 4 cut(s) | 152, 155, 181, 464 |
| HpyAV | CCTTC | 5 cut(s) | 811, 1464, 1930, 3131, 3293 |
| HpyCH4III | ACNGT | 7 cut(s) | 65, 229, 892, 1006, 2200, 2980, 3437 |
| HpyCH4IV | ACGT | 6 cut(s) | 104, 218, 612, 1650, 2948, 3429 |
| HpySE526I | ACGT | 6 cut(s) | 104, 218, 612, 1650, 2948, 3429 |
| Hsp92I | GRCGYC | 3 cut(s) | 737, 1446, 2580 |
| HspAI | GCGC | 6 cut(s) | 737, 920, 989, 1011, 1566, 2580 |
| KasI | GGCGCC | 2 cut(s) | 736, 2579 |
| Kpn2I | TCCGGA | 1 cut(s) | 346 |
| Ksp22I | TGATCA | 1 cut(s) | 411 |
| KspI | CCGCGG | 2 cut(s) | 918, 3067 |
| Kzo9I | GATC | 9 cut(s) | 411, 863, 1159, 1822, 1858, 1917, 2129, 2645, 2699 |
| LguI | GCTCTTC | 1 cut(s) | 1308 |
| LweI | GCATC | 6 cut(s) | 20, 1120, 1318, 1363, 2054, 2164 |
| MaeII | ACGT | 6 cut(s) | 104, 218, 612, 1650, 2948, 3429 |
| MaeIII | GTNAC | 8 cut(s) | 31, 381, 624, 685, 1217, 1498, 1949, 2200 |
| MalI | GATC | 9 cut(s) | 413, 865, 1161, 1824, 1860, 1919, 2131, 2647, 2701 |
| MbiI | CCGCTC | 3 cut(s) | 3270, 3296, 3422 |
| MboI | GATC | 9 cut(s) | 411, 863, 1159, 1822, 1858, 1917, 2129, 2645, 2699 |
| MflI | RGATCY | 2 cut(s) | 1822, 2699 |
| MhlI | GDGCHC | 7 cut(s) | 450, 1662, 1700, 2092, 2896, 3314, 3429 |
| MlsI | TGGCCA | 4 cut(s) | 645, 755, 1359, 2667 |
| MluCI | AATT | 9 cut(s) | 294, 1067, 1419, 1492, 1900, 1978, 1984, 2331, 2937 |
| MluNI | TGGCCA | 4 cut(s) | 645, 755, 1359, 2667 |
| Mly113I | GGCGCC | 2 cut(s) | 737, 2580 |
| MlyI | GAGTC | 4 cut(s) | 337, 1258, 1946, 2065 |
| MmeI | TCCRAC | 4 cut(s) | 1433, 1474, 1552, 2979 |
| Mox20I | TGGCCA | 4 cut(s) | 645, 755, 1359, 2667 |
| Mph1103I | ATGCAT | 1 cut(s) | 1333 |
| MroI | TCCGGA | 1 cut(s) | 346 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1940 |
| MscI | TGGCCA | 4 cut(s) | 645, 755, 1359, 2667 |
| MseI | TTAA | 5 cut(s) | 1710, 2057, 2399, 2847, 3324 |
| MslI | CAYNNNNRTG | 4 cut(s) | 63, 116, 434, 1653 |
| Msp20I | TGGCCA | 4 cut(s) | 645, 755, 1359, 2667 |
| MspA1I | CMGCKG | 5 cut(s) | 203, 917, 2696, 2828, 3066 |
| Mva1269I | GAATGC | 1 cut(s) | 727 |
| MvaI | CCWGG | 6 cut(s) | 291, 717, 1536, 1845, 2584, 2712 |
| MvnI | CGCG | 4 cut(s) | 140, 917, 1664, 3066 |
| NarI | GGCGCC | 2 cut(s) | 737, 2580 |
| NciI | CCSGG | 6 cut(s) | 1037, 1173, 1201, 2093, 2889, 3002 |
| NcoI | CCATGG | 3 cut(s) | 235, 646, 2668 |
| NdeI | CATATG | 1 cut(s) | 1306 |
| NdeII | GATC | 9 cut(s) | 411, 863, 1159, 1822, 1858, 1917, 2129, 2645, 2699 |
| NheI | GCTAGC | 3 cut(s) | 695, 2213, 2717 |
| NlaIV | GGNNCC | 8 cut(s) | 738, 1697, 1824, 2089, 2581, 2620, 2701, 3311 |
| NmeAIII | GCCGAG | 2 cut(s) | 286, 510 |
| NmuCI | GTSAC | 5 cut(s) | 624, 1217, 1498, 1949, 2200 |
| NsiI | ATGCAT | 1 cut(s) | 1333 |
| NspI | RCATGY | 4 cut(s) | 95, 211, 1820, 2793 |
| OliI | CACNNNNGTG | 3 cut(s) | 63, 434, 1653 |
| PaeI | GCATGC | 1 cut(s) | 2793 |
| PaeR7I | CTCGAG | 1 cut(s) | 747 |
| PagI | TCATGA | 2 cut(s) | 1722, 3336 |
| PciSI | GCTCTTC | 1 cut(s) | 1308 |
| PctI | GAATGC | 1 cut(s) | 727 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1940 |
| PfeI | GAWTC | 8 cut(s) | 197, 617, 1117, 1183, 1394, 1400, 2156, 2639 |
| PflFI | GACNNNGTC | 1 cut(s) | 158 |
| PfoI | TCCNGGA | 2 cut(s) | 2710, 2887 |
| PinAI | ACCGGT | 1 cut(s) | 1459 |
| PleI | GAGTC | 4 cut(s) | 337, 1257, 1946, 2065 |
| PluTI | GGCGCC | 2 cut(s) | 740, 2583 |
| PmaCI | CACGTG | 2 cut(s) | 1651, 3430 |
| PmlI | CACGTG | 2 cut(s) | 1651, 3430 |
| PpsI | GAGTC | 4 cut(s) | 337, 1257, 1946, 2065 |
| Ppu21I | YACGTR | 3 cut(s) | 219, 1651, 3430 |
| PshAI | GACNNNNGTC | 1 cut(s) | 2487 |
| Psp124BI | GAGCTC | 1 cut(s) | 450 |
| Psp6I | CCWGG | 6 cut(s) | 289, 715, 1534, 1843, 2582, 2710 |
| PspCI | CACGTG | 2 cut(s) | 1651, 3430 |
| PspFI | CCCAGC | 3 cut(s) | 1553, 1811, 3010 |
| PspGI | CCWGG | 6 cut(s) | 289, 715, 1534, 1843, 2582, 2710 |
| PspN4I | GGNNCC | 8 cut(s) | 738, 1697, 1824, 2089, 2581, 2620, 2701, 3311 |
| PspPI | GGNCC | 8 cut(s) | 476, 596, 959, 1487, 1574, 1666, 2618, 2933 |
| PspXI | VCTCGAGB | 1 cut(s) | 747 |
| PsuI | RGATCY | 2 cut(s) | 1822, 2699 |
| PsyI | GACNNNGTC | 1 cut(s) | 158 |
| PvuII | CAGCTG | 3 cut(s) | 203, 2696, 2828 |
| RseI | CAYNNNNRTG | 4 cut(s) | 63, 116, 434, 1653 |
| SacI | GAGCTC | 1 cut(s) | 450 |
| SacII | CCGCGG | 2 cut(s) | 918, 3067 |
| SapI | GCTCTTC | 1 cut(s) | 1308 |
| SaqAI | TTAA | 5 cut(s) | 1710, 2057, 2399, 2847, 3324 |
| Sau3AI | GATC | 9 cut(s) | 411, 863, 1159, 1822, 1858, 1917, 2129, 2645, 2699 |
| Sau96I | GGNCC | 8 cut(s) | 476, 596, 959, 1487, 1574, 1666, 2618, 2933 |
| ScaI | AGTACT | 1 cut(s) | 1512 |
| SchI | GAGTC | 4 cut(s) | 337, 1258, 1946, 2065 |
| SduI | GDGCHC | 7 cut(s) | 450, 1662, 1700, 2092, 2896, 3314, 3429 |
| SfaNI | GCATC | 6 cut(s) | 20, 1120, 1318, 1363, 2054, 2164 |
| SfcI | CTRYAG | 1 cut(s) | 1344 |
| SfoI | GGCGCC | 2 cut(s) | 738, 2581 |
| Sfr274I | CTCGAG | 1 cut(s) | 747 |
| Sfr303I | CCGCGG | 2 cut(s) | 918, 3067 |
| SgrAI | CRCCGGYG | 1 cut(s) | 431 |
| SgrBI | CCGCGG | 2 cut(s) | 918, 3067 |
| SinI | GGWCC | 6 cut(s) | 476, 596, 959, 1487, 1574, 2618 |
| SlaI | CTCGAG | 1 cut(s) | 747 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 63, 116, 434, 1653 |
| SmlI | CTYRAG | 4 cut(s) | 747, 1954, 2554, 3287 |
| SmoI | CTYRAG | 4 cut(s) | 747, 1954, 2554, 3287 |
| SphI | GCATGC | 1 cut(s) | 2793 |
| Sse9I | AATT | 9 cut(s) | 294, 1067, 1419, 1492, 1900, 1978, 1984, 2331, 2937 |
| SspDI | GGCGCC | 2 cut(s) | 736, 2579 |
| SstI | GAGCTC | 1 cut(s) | 450 |
| StyI | CCWWGG | 8 cut(s) | 235, 646, 703, 992, 1051, 2026, 2504, 2668 |
| TaaI | ACNGT | 7 cut(s) | 65, 229, 892, 1006, 2200, 2980, 3437 |
| TaiI | ACGT | 6 cut(s) | 107, 221, 615, 1653, 2951, 3432 |
| TaqI | TCGA | 9 cut(s) | 14, 147, 279, 533, 748, 1151, 1764, 1800, 2395 |
| TaqII | GACCGA | 2 cut(s) | 1236, 1504 |
| TasI | AATT | 9 cut(s) | 294, 1067, 1419, 1492, 1900, 1978, 1984, 2331, 2937 |
| TatI | WGTACW | 2 cut(s) | 1510, 2061 |
| TauI | GCSGC | 9 cut(s) | 674, 742, 917, 920, 1667, 1682, 1706, 3281, 3296 |
| TfiI | GAWTC | 8 cut(s) | 197, 617, 1117, 1183, 1394, 1400, 2156, 2639 |
| Tru1I | TTAA | 5 cut(s) | 1710, 2057, 2399, 2847, 3324 |
| Tru9I | TTAA | 5 cut(s) | 1710, 2057, 2399, 2847, 3324 |
| TseFI | GTSAC | 5 cut(s) | 624, 1217, 1498, 1949, 2200 |
| Tsp45I | GTSAC | 5 cut(s) | 624, 1217, 1498, 1949, 2200 |
| TspDTI | ATGAA | 7 cut(s) | 17, 621, 1556, 2357, 2427, 2619, 2643 |
| TspGWI | ACGGA | 3 cut(s) | 10, 1451, 2134 |
| Tth111I | GACNNNGTC | 1 cut(s) | 158 |
| VpaK11BI | GGWCC | 6 cut(s) | 476, 596, 959, 1487, 1574, 2618 |
| XagI | CCTNNNNNAGG | 3 cut(s) | 1673, 2709, 3287 |
| XapI | RAATTY | 5 cut(s) | 294, 1067, 1492, 1978, 2331 |
| XbaI | TCTAGA | 2 cut(s) | 1481, 2008 |
| XceI | RCATGY | 4 cut(s) | 95, 211, 1820, 2793 |
| XhoI | CTCGAG | 1 cut(s) | 747 |
| XmiI | GTMKAC | 2 cut(s) | 1854, 2445 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1940 |
| ZrmI | AGTACT | 1 cut(s) | 1512 |
| Zsp2I | ATGCAT | 1 cut(s) | 1333 |