Rh6BG492900

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001634
Physical Location & Seq
Reverse (-)
69534771 .. 69537926
3156 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG492900.1

Sequence Viewer

Length: 1203 bp
ATGGCCAAGGGAGTATTTGTGTCATGTTCTGCCGGAAATGATGGGCCAATTTCCTATACCTCATCCAACAATGCACCTTGGATAACCACTGTGGGTGCTGGAACACTAGACCGGGACTTCCCAGCTTATGTTTGTCTTGGAAATGGAAAGAAATACAGAGGTATAGCACTTGACCGCGGTATAGTATCATTATCTAGTGGATTACATCCCCTTGTATATGCTTCTAATGCAAGTAACTCCACAAGTGGTGATCAATGCTCTCCTAACAGTTTGATTCCTCAGATGGTTGCTGGGAAAATTGTGGTATGCGATCGGGGAGGAAGTAGGGTCAGAAAGAGTGTGGTGGTAAAAAAGGCTGGTGGGGTAGGGATGATTATGGCAAACATTGAAGGTTATGGAGAAGAACTTGTAGCCGACCCATATCTCTTGCCAACTGCATTGGTTGGCAAGAAAGCTGGTGATGCAATAAGAAGATATATTGCCTCGCATGCTAATCCGGAAGCCACATTGGTTTTCAGAAAGACTCAGTTGGGTATCGAACCCTCACCAGTGGTGGCAGCATTCAGCTCTAGAGGACCCAATCCAGTCGCTACATCACTACTCAAGCCAGACTTGATAGCACCAGGAGTTAATATTCTAGCTGGGTGGACTGGTGCAGATGGACCAACTGGACTACCTGAAGACAAAAAGAGGGTTAGCTACAACATCATTTCAGGTACATCCATGTCATGCCCTCATGTGAGCGGGCTTGCCGCCCTTCTCAAGGCCGCTCACCCAAAATGGAGCCCTGCTGCCATTAAATCTGCTCTCATGACCACATCCTATACAAGAAGAAATGGAAAACCAATCAAAGATGTTGCTACTGGAAAGCCTGCAACACCATTTGATTATGGAGCTGGGCACGTGAACCCAGTTGGAGCCCTTGACCCTGGCCTTATTTATGATCTAGCTGTGGAGGACTACCTAGGCTTCCTCTGTGCCATGAATTACACGACGAAAGATATCAAGAAACTCACTCACAGAGACTCCACCTGTGACTCAAGAAAGAAATACAGAGTTCGAGATTTCAACTACCCATCTCTTGCTGCTACCAGTCGGGGGTGGATCTGTCAATTACACAAGGACTCTAACCAATGTGGGTACCCCAGCAAGATACAAGGTCCGAGTGACTACAAAGTCCCCATTAGTGAAGATCTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

400

Amino Acids

42.34

Weight (kDa)

9.23

Isoelectric Point (pI)

33.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 2 - 300 3e-24 Subtilase family
PA PF02225 70 - 156 5.7e-11 PA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1175
Acc65I GGTACC 1 cut(s) 1140
AccB1I GGYRCC 1 cut(s) 1140
AccBSI CCGCTC 2 cut(s) 744, 770
AccII CGCG 1 cut(s) 177
AccIII TCCGGA 1 cut(s) 496
AciI CCGC 5 cut(s) 175, 177, 744, 753, 768
AclWI GGATC 1 cut(s) 1112
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 699
AcvI CACGTG 1 cut(s) 904
AfaI GTAC 2 cut(s) 718, 1142
AfiI CCNNNNNNNGG 2 cut(s) 763, 1098
AgsI TTSAA 2 cut(s) 389, 1069
AjnI CCWGG 2 cut(s) 622, 928
AluBI AGCT 7 cut(s) 125, 455, 567, 641, 699, 896, 950
AluI AGCT 7 cut(s) 125, 455, 567, 641, 699, 896, 950
Alw26I GTCTC 1 cut(s) 1017
AlwI GGATC 1 cut(s) 1112
Aor13HI TCCGGA 1 cut(s) 496
AoxI GGCC 4 cut(s) 3, 44, 765, 931
ApeKI GCWGC 3 cut(s) 557, 791, 1085
Asp718I GGTACC 1 cut(s) 1140
AspA2I CCTAGG 1 cut(s) 964
AspS9I GGNCC 4 cut(s) 44, 575, 662, 1160
AsuC2I CCSGG 1 cut(s) 113
AsuHPI GGTGA 4 cut(s) 260, 470, 537, 764
AvaII GGWCC 3 cut(s) 575, 662, 1160
AvrII CCTAGG 1 cut(s) 964
BaeGI GKGCMC 1 cut(s) 903
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 1140
BanII GRGCYC 2 cut(s) 788, 922
BbrPI CACGTG 1 cut(s) 904
BbsI GAAGAC 1 cut(s) 687
BbvI GCAGC 3 cut(s) 569, 778, 1072
BccI CCATC 4 cut(s) 35, 277, 653, 1084
BciT130I CCWGG 2 cut(s) 624, 930
BclI TGATCA 1 cut(s) 250
BcnI CCSGG 1 cut(s) 113
BcoDI GTCTC 1 cut(s) 1017
BfaI CTAG 6 cut(s) 107, 195, 570, 638, 947, 965
BglII AGATCT 1 cut(s) 1192
BisI GCNGC 5 cut(s) 558, 753, 768, 792, 1086
BlnI CCTAGG 1 cut(s) 964
BlsI GCNGC 5 cut(s) 559, 754, 769, 793, 1087
Bme1390I CCNGG 3 cut(s) 113, 624, 930
Bme18I GGWCC 3 cut(s) 575, 662, 1160
BmgT120I GGNCC 4 cut(s) 44, 575, 662, 1160
BmiI GGNNCC 4 cut(s) 577, 785, 919, 1142
BmrFI CCNGG 3 cut(s) 113, 624, 930
BmrI ACTGGG 1 cut(s) 905
BmsI GCATC 1 cut(s) 451
BmuI ACTGGG 1 cut(s) 905
BpiI GAAGAC 1 cut(s) 687
BpuEI CTTGAG 3 cut(s) 587, 746, 1024
BpuMI CCSGG 1 cut(s) 113
BsaAI YACGTR 1 cut(s) 904
BsaJI CCNNGG 5 cut(s) 6, 77, 175, 928, 964
BsaWI WCCGGW 1 cut(s) 496
BsaXI ACNNNNNCTCC 3 cut(s) 33, 1010, 1040
Bsc4I CCNNNNNNNGG 2 cut(s) 763, 1098
Bse1I ACTGG 7 cut(s) 548, 584, 655, 673, 868, 911, 1092
BseAI TCCGGA 1 cut(s) 496
BseBI CCWGG 2 cut(s) 624, 930
BseDI CCNNGG 5 cut(s) 6, 77, 175, 928, 964
BseGI GGATG 5 cut(s) 62, 205, 375, 719, 818
BseLI CCNNNNNNNGG 2 cut(s) 763, 1098
BseMII CTCAG 2 cut(s) 293, 539
BseNI ACTGG 7 cut(s) 548, 584, 655, 673, 868, 911, 1092
BseSI GKGCMC 1 cut(s) 903
BseXI GCAGC 3 cut(s) 569, 778, 1072
BseYI CCCAGC 5 cut(s) 121, 290, 641, 896, 1145
BsgI GTGCAG 1 cut(s) 675
Bsh1236I CGCG 1 cut(s) 177
Bsh1285I CGRYCG 1 cut(s) 313
BshFI GGCC 4 cut(s) 5, 46, 767, 933
BshNI GGYRCC 1 cut(s) 1140
BsiEI CGRYCG 1 cut(s) 313
BsiSI CCGG 3 cut(s) 33, 112, 497
BslFI GGGAC 2 cut(s) 128, 1163
BslI CCNNNNNNNGG 2 cut(s) 763, 1098
BsmAI GTCTC 1 cut(s) 1017
BsmFI GGGAC 2 cut(s) 128, 1163
BsmI GAATGC 1 cut(s) 560
BsnI GGCC 4 cut(s) 5, 46, 767, 933
Bsp1286I GDGCHC 3 cut(s) 788, 903, 922
Bsp13I TCCGGA 1 cut(s) 496
Bsp143I GATC 5 cut(s) 250, 310, 943, 1104, 1192
BspACI CCGC 5 cut(s) 175, 177, 744, 753, 768
BspANI GGCC 4 cut(s) 5, 46, 767, 933
BspCNI CTCAG 2 cut(s) 292, 538
BspEI TCCGGA 1 cut(s) 496
BspFNI CGCG 1 cut(s) 177
BspHI TCATGA 1 cut(s) 810
BspLI GGNNCC 4 cut(s) 577, 785, 919, 1142
BspPI GGATC 1 cut(s) 1112
BspT107I GGYRCC 1 cut(s) 1140
BsrBI CCGCTC 2 cut(s) 744, 770
BsrI ACTGG 7 cut(s) 548, 584, 655, 673, 868, 911, 1092
BssECI CCNNGG 5 cut(s) 6, 77, 175, 928, 964
BssMI GATC 5 cut(s) 250, 310, 943, 1104, 1192
BssT1I CCWWGG 3 cut(s) 6, 77, 964
Bst2UI CCWGG 2 cut(s) 624, 930
Bst4CI ACNGT 2 cut(s) 91, 269
BstBAI YACGTR 1 cut(s) 904
BstC8I GCNNGC 4 cut(s) 489, 746, 750, 873
BstDEI CTNAG 2 cut(s) 279, 525
BstDSI CCRYGG 1 cut(s) 175
BstENI CCTNNNNNAGG 1 cut(s) 761
BstF5I GGATG 5 cut(s) 62, 205, 375, 719, 818
BstFNI CGCG 1 cut(s) 177
BstKTI GATC 5 cut(s) 253, 313, 946, 1107, 1195
BstMAI GTCTC 1 cut(s) 1017
BstMBI GATC 5 cut(s) 250, 310, 943, 1104, 1192
BstMCI CGRYCG 1 cut(s) 313
BstMWI GCNNNNNNNGC 3 cut(s) 227, 461, 488
BstNI CCWGG 2 cut(s) 624, 930
BstNSI RCATGY 1 cut(s) 491
BstSCI CCNGG 3 cut(s) 111, 622, 928
BstSLI GKGCMC 1 cut(s) 903
BstUI CGCG 1 cut(s) 177
BstV1I GCAGC 3 cut(s) 569, 778, 1072
BstV2I GAAGAC 1 cut(s) 687
BstX2I RGATCY 2 cut(s) 1104, 1192
BstYI RGATCY 2 cut(s) 1104, 1192
BsuRI GGCC 4 cut(s) 5, 46, 767, 933
BtgI CCRYGG 1 cut(s) 175
BtsCI GGATG 5 cut(s) 62, 205, 375, 719, 818
BtsIMutI CAGTG 2 cut(s) 87, 555
Cac8I GCNNGC 4 cut(s) 489, 746, 750, 873
CciI TCATGA 1 cut(s) 810
Cfr13I GGNCC 4 cut(s) 44, 575, 662, 1160
Cfr42I CCGCGG 1 cut(s) 178
Csp6I GTAC 2 cut(s) 717, 1141
CviAII CATG 7 cut(s) 24, 488, 724, 729, 737, 811, 982
CviQI GTAC 2 cut(s) 717, 1141
DdeI CTNAG 2 cut(s) 279, 525
DpnI GATC 5 cut(s) 252, 312, 945, 1106, 1194
DpnII GATC 5 cut(s) 250, 310, 943, 1104, 1192
DrdI GACNNNNNNGTC 1 cut(s) 1175
DseDI GACNNNNNNGTC 1 cut(s) 1175
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 3 cut(s) 6, 77, 964
Eco24I GRGCYC 2 cut(s) 788, 922
Eco32I GATATC 1 cut(s) 1003
Eco47I GGWCC 3 cut(s) 575, 662, 1160
Eco57I CTGAAG 1 cut(s) 699
Eco72I CACGTG 1 cut(s) 904
EcoNI CCTNNNNNAGG 1 cut(s) 761
EcoO109I RGGNCCY 1 cut(s) 575
EcoRII CCWGG 2 cut(s) 622, 928
EcoRV GATATC 1 cut(s) 1003
EcoT14I CCWWGG 3 cut(s) 6, 77, 964
EcoT38I GRGCYC 2 cut(s) 788, 922
ErhI CCWWGG 3 cut(s) 6, 77, 964
FaeI CATG 7 cut(s) 27, 491, 727, 732, 740, 814, 985
FalI AAGNNNNNCTT 2 cut(s) 596, 628
FaqI GGGAC 2 cut(s) 128, 1163
FatI CATG 7 cut(s) 23, 487, 723, 728, 736, 810, 981
FauI CCCGC 1 cut(s) 737
FbaI TGATCA 1 cut(s) 250
Fnu4HI GCNGC 5 cut(s) 558, 753, 768, 792, 1086
FokI GGATG 5 cut(s) 49, 192, 382, 706, 805
FriOI GRGCYC 2 cut(s) 788, 922
Fsp4HI GCNGC 5 cut(s) 558, 753, 768, 792, 1086
FspBI CTAG 6 cut(s) 107, 195, 570, 638, 947, 965
GluI GCNGC 5 cut(s) 558, 753, 768, 792, 1086
GsaI CCCAGC 5 cut(s) 125, 294, 645, 900, 1149
HaeIII GGCC 4 cut(s) 5, 46, 767, 933
HapII CCGG 3 cut(s) 33, 112, 497
Hin1II CATG 7 cut(s) 27, 491, 727, 732, 740, 814, 985
HinfI GANTC 5 cut(s) 274, 523, 1025, 1037, 1124
HpaII CCGG 3 cut(s) 33, 112, 497
HphI GGTGA 4 cut(s) 260, 470, 537, 764
Hpy166II GTNNAC 2 cut(s) 648, 907
Hpy188I TCNGA 4 cut(s) 282, 332, 518, 1164
Hpy188III TCNNGA 7 cut(s) 497, 570, 811, 1006, 1041, 1061, 1196
Hpy8I GTNNAC 2 cut(s) 648, 907
Hpy99I CGWCG 1 cut(s) 997
HpyAV CCTTC 2 cut(s) 383, 767
HpyCH4III ACNGT 2 cut(s) 91, 269
HpyCH4IV ACGT 1 cut(s) 903
HpyCH4V TGCA 6 cut(s) 74, 230, 437, 464, 656, 875
HpyF10VI GCNNNNNNNGC 3 cut(s) 227, 461, 488
HpyF3I CTNAG 2 cut(s) 279, 525
HpySE526I ACGT 1 cut(s) 903
Hsp92II CATG 7 cut(s) 27, 491, 727, 732, 740, 814, 985
Kpn2I TCCGGA 1 cut(s) 496
KpnI GGTACC 1 cut(s) 1144
Ksp22I TGATCA 1 cut(s) 250
KspI CCGCGG 1 cut(s) 178
Kzo9I GATC 5 cut(s) 250, 310, 943, 1104, 1192
LmnI GCTCC 3 cut(s) 783, 893, 917
Lsp1109I GCAGC 3 cut(s) 569, 778, 1072
LweI GCATC 1 cut(s) 451
MaeI CTAG 6 cut(s) 107, 195, 570, 638, 947, 965
MaeII ACGT 1 cut(s) 903
MaeIII GTNAC 3 cut(s) 233, 1034, 1166
MalI GATC 5 cut(s) 252, 312, 945, 1106, 1194
MbiI CCGCTC 2 cut(s) 744, 770
MboI GATC 5 cut(s) 250, 310, 943, 1104, 1192
MboII GAAGA 5 cut(s) 413, 483, 692, 843, 1202
MflI RGATCY 2 cut(s) 1104, 1192
MhlI GDGCHC 3 cut(s) 788, 903, 922
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 48, 297, 985, 1112
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 4 cut(s) 517, 1019, 1031, 1118
MmeI TCCRAC 2 cut(s) 90, 895
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 496
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 630, 798, 1201
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 177
MspI CCGG 3 cut(s) 33, 112, 497
MspR9I CCNGG 3 cut(s) 113, 624, 930
Mva1269I GAATGC 1 cut(s) 560
MvaI CCWGG 2 cut(s) 624, 930
MvnI CGCG 1 cut(s) 177
MwoI GCNNNNNNNGC 3 cut(s) 227, 461, 488
NciI CCSGG 1 cut(s) 113
NdeII GATC 5 cut(s) 250, 310, 943, 1104, 1192
NlaIII CATG 7 cut(s) 27, 491, 727, 732, 740, 814, 985
NlaIV GGNNCC 4 cut(s) 577, 785, 919, 1142
NmuCI GTSAC 2 cut(s) 1034, 1166
NspI RCATGY 1 cut(s) 491
PaeI GCATGC 1 cut(s) 491
PagI TCATGA 1 cut(s) 810
PctI GAATGC 1 cut(s) 560
PfeI GAWTC 1 cut(s) 274
PkrI GCNGC 5 cut(s) 559, 754, 769, 793, 1087
Ple19I CGATCG 1 cut(s) 313
PleI GAGTC 4 cut(s) 517, 1019, 1031, 1118
PmaCI CACGTG 1 cut(s) 904
PmlI CACGTG 1 cut(s) 904
PpsI GAGTC 4 cut(s) 517, 1019, 1031, 1118
Ppu21I YACGTR 1 cut(s) 904
PpuMI RGGWCCY 1 cut(s) 575
Psp5II RGGWCCY 1 cut(s) 575
Psp6I CCWGG 2 cut(s) 622, 928
PspCI CACGTG 1 cut(s) 904
PspFI CCCAGC 5 cut(s) 121, 290, 641, 896, 1145
PspGI CCWGG 2 cut(s) 622, 928
PspN4I GGNNCC 4 cut(s) 577, 785, 919, 1142
PspPI GGNCC 4 cut(s) 44, 575, 662, 1160
PspPPI RGGWCCY 1 cut(s) 575
PsuI RGATCY 2 cut(s) 1104, 1192
PvuI CGATCG 1 cut(s) 313
RsaI GTAC 2 cut(s) 718, 1142
RsaNI GTAC 2 cut(s) 717, 1141
SacII CCGCGG 1 cut(s) 178
SaqAI TTAA 3 cut(s) 630, 798, 1201
SatI GCNGC 5 cut(s) 558, 753, 768, 792, 1086
Sau3AI GATC 5 cut(s) 250, 310, 943, 1104, 1192
Sau96I GGNCC 4 cut(s) 44, 575, 662, 1160
SchI GAGTC 4 cut(s) 517, 1019, 1031, 1118
ScrFI CCNGG 3 cut(s) 113, 624, 930
SduI GDGCHC 3 cut(s) 788, 903, 922
SfaNI GCATC 1 cut(s) 451
Sfr303I CCGCGG 1 cut(s) 178
SgrBI CCGCGG 1 cut(s) 178
SinI GGWCC 3 cut(s) 575, 662, 1160
SmlI CTYRAG 3 cut(s) 602, 761, 1039
SmoI CTYRAG 3 cut(s) 602, 761, 1039
SphI GCATGC 1 cut(s) 491
Sse9I AATT 4 cut(s) 48, 297, 985, 1112
SsiI CCGC 5 cut(s) 175, 177, 744, 753, 768
SspI AATATT 1 cut(s) 634
SspMI CTAG 6 cut(s) 107, 195, 570, 638, 947, 965
StyD4I CCNGG 3 cut(s) 111, 622, 928
StyI CCWWGG 3 cut(s) 6, 77, 964
TaaI ACNGT 2 cut(s) 91, 269
TaiI ACGT 1 cut(s) 906
TaqI TCGA 2 cut(s) 537, 1060
TasI AATT 4 cut(s) 48, 297, 985, 1112
TauI GCSGC 2 cut(s) 755, 770
TfiI GAWTC 1 cut(s) 274
Tru1I TTAA 3 cut(s) 630, 798, 1201
Tru9I TTAA 3 cut(s) 630, 798, 1201
TscAI CASTG 2 cut(s) 94, 555
TseFI GTSAC 2 cut(s) 1034, 1166
TseI GCWGC 3 cut(s) 557, 791, 1085
Tsp45I GTSAC 2 cut(s) 1034, 1166
TspDTI ATGAA 1 cut(s) 998
TspRI CASTG 2 cut(s) 94, 555
VpaK11BI GGWCC 3 cut(s) 575, 662, 1160
XagI CCTNNNNNAGG 1 cut(s) 761
XbaI TCTAGA 1 cut(s) 569
XceI RCATGY 1 cut(s) 491
XmaJI CCTAGG 1 cut(s) 964
XspI CTAG 6 cut(s) 107, 195, 570, 638, 947, 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.