Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0001634

Physical Location & Seq

Genomic Coordinates Reverse (-)

27114513 .. 27116906

Gene Structure 2394 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1292900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2394 bp

ATGGTCCCTCACTCCCTTCTAAACACAGGGAGTGAAGAATATACAGTGACTGAGCTTTCAGCTGCTAAGCTGAACAGAATGGCCATTATGTCTACCTCTTTTGTGCTTCTTCTCCTTCTGGCTCTCTGCCGATTCTCCACTGCAGCAGCGGTGGCGGACAATACCGATGTCTCCCACCCCAAAACCACGCCTTCTACTTACATCGTCCACATGGCCAAATCCCAAATGCCCGCGAGTTTCGAGCATCACACCCACTGGTACGATTCGTCGCTCAAATCCGTCTCCGACTCGGCCGAAATGCTCTACACTTACACCTCTGCCATCCACGGCTTCTCCACCAGGCTGACGCCGGAGCAGGCAGCGTCGCTCAGTTCACAACCCGGAGTACTCTCCGTGCTTCCTGAACTCAAGTACGAGCTCCACACGACTCGGACCCCCGAGTTCCTCGGACTCGGCCAGACCACCGAGACAATGCCCCAGTCAGACTCGGCCGGTGACGTCATCATCGGAGTACTGGACACCGGCGTCTGGCCCGAGAGCAAGAGCTTCGATGACACCGGATTCGGACCCGTGCCCTCCTCCTGGAATGGCACGTGCGAATCTGGAACCAACTTCAACTCCTCCAATTGTAACCGCAAACTAATCGGCGCCAGGTACTTCGCCAAAGGCTACGAGGCCACGCTGGGCCCCATCGAGGAGTCCAAAGAATCCAAATCCCCCCGCGACGACGACGGCCACGGCACTCACACCGCGACAACCGCCGCTGGCTCCTTTGTCCCCGGCGCGAGTCTATTAGGGTACGCGCCAGGAACCGCCCGTGGGATGGCCCCGCGCGCCCGAATCGCCGCCTACAAGGTGTGCTGGGTCGGCGGCTGTTTCAGCTCCGACATTCTTGCCGCCATGGACAAAGCAATCGACGACAACGTCAATGTCCTCTCGATGTCGCTCGGCGGCGGGAATTCGGACTATTTCCGTGACAGCGTGGCGATGGGAGCTTTCTCAGCGATGGAGAAGGGAATCTTAATCTCGTGCTCTGCTGGAAACGCGGGCCCCAGCGCTTATAGCTTGTCGAACTCGGCTCCATGGATCACAACCGTGGGAGCAGGCACACTGGATCGAGATTTCCCCGCTTTCGTCAGCCTCGGTAACGGCAAAAACTTCTCCGGTGTTTCGCTTTACCGCGACAACTCCAACTCAATGCCAAGCGCCTTGACGCCGTTTATTTATGCCGGTAACGCGAGCAACGCCACTTCCGGGAATCTATGCATGATGGGGACTTTGAGTCCCCAACAAGTGAAGGGGAAGATAGTGATGTGCGACCGCGGAGTCAACGCTAGAGTCCAAAAAGGGGCCGTGGTCAAAGCTGCGGGAGGATTGGGCATGGTTTTGGCTAACACCGCCGCGAACGGCGAAGAACTGGTGGCTGACGCTCATTTGTTACCGGCCACGTCTGTGGGTCAGAGAAACAGCGACGTCATCAAGAGCTATTTATTCTCGGATCCGAACCCGACCGCCACGATTTTATTCGAGGGGACAAAAGTCGGAGTCCAGCCATCTCCGGTAGTCGCGGCGTTTAGTTCTCGGGGCCCAAATTCGATCACAGCGGATATACTGAAACCCGATATAGTTGCTCCAGGTGTCAACATCCTAGCGGGGTGGTCAGGTGCGGTAGGACCTACTGGTCTTGCGATCGATGCGAGACGCGTGGCGTTCAATATTATATCCGGGACGTCGATGTCTTGCCCACATGTGAGTGGGCTGGCGGCGCTGCTGAAGGGGGCGCATCCGGAGTGGAGCCCGGCGGCGATTCGATCGGCGCTCATGACCACGGCGTATACGGCGTACAAGAACGGGCAGAAGTTGCAAGATGTGGCTACCGGGAAGCCATCCACCCCGTTCGATCACGGTGCAGGACACGTGGATCCTATATCGGCCCTCAACCCGGGACTTGTCTATGATCTAACGGTGGACGATTATCTGAACTTTCTGTGTGCGTTGAATTACTCGGCGTCTGAGATCAGCACCCTGGCGAAAAGATCGTACACTTGCGACGAGAAAAAGAGGTACAGCGTGAGGGACCTTAACTACCCGTCGTTCGCGGTGAACTTCGAGGGCAGAACGACGTCGACGAATGTAGCGAAGTACACGAGGACGGTGACGAATGTGGGGCCGGCGGGAACGTACAAGGCAACAGTGACGTCGGACAACCCGTCGGTGAAGATAACGGTGGAGCCCAACACGCTGAGTTTTGGTCAGGTGAACGAGAAGAAAGCCTATACGGTGACGTTTAGTGCTTCCGGTTCGGCGCCGGTGAATGCGAACAGTTTCGGGAGGCTGGAGTGGTCGGACGGCAAGCATGTTGTGGGAAGCCCAATAGCAATGAGCTGGAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0006508 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009505 GO:0009791 GO:0009845 GO:0009987 GO:0010154 GO:0010191 GO:0010214 GO:0016787 GO:0017171 GO:0019538 GO:0022414 GO:0030312 GO:0032501 GO:0032502 GO:0032504 GO:0043170 GO:0044237 GO:0044238 GO:0044464 GO:0048046 GO:0048316 GO:0048359 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0061458 GO:0070011 GO:0071704 GO:0071944 GO:0080001 GO:0090351 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

798

Amino Acids

83.78

Weight (kDa)

6.36

Isoelectric Point (pI)

30.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	66 - 141	2.2e-20	Peptidase inhibitor I9
Peptidase_S8	PF00082	166 - 619	2.5e-50	Subtilase family
PA	PF02225	421 - 490	1.7e-11	PA domain
fn3_6	PF17766	693 - 793	1.4e-27	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001634)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G67360
fragaria_vesca	FvH4_1g17150 FvH4_2g33321 FvH4_2g33321 FvH4_2g33321 FvH4_2g33321 FvH4_2g33340
malus_domestica	MD08G1087900.v1.1 MD08G1088000.v1.1 MD15G1072700.v1.1 MD15G1292900.v1.1
prunus_persica	Prupe.1G425400_v2.0.a1 Prupe.1G425500_v2.0.a1 Prupe.1G425600_v2.0.a1 Prupe.1G425700_v2.0.a1 Prupe.6G224600_v2.0.a1
pyrus_communis	pycom08g07160 pycom15g06800 pycom15g25580
rosa_chinensis	RchiOBHm_Chr2g0107331 RchiOBHm_Chr6g0309241 RchiOBHm_Chr6g0309251
rosa_laevigata	RLG00000010577 RLG00000010578 RLG00000017570
rosa_multiflora	Rmu_co8090004.1_g000001 Rmu_co8331721.1_g000001 Rmu_sc0007504.1_g000002 Rmu_sc0015692.1_g000006
rosa_roxburghii	Rroxscaffold_2G00136430 Rroxscaffold_7G00159520 Rroxscaffold_7G00159530
rosa_rugosa	Rorug02G0142100 Rorug06G0370500 Rorug06G0370600
rosa_samantha	Rh2AG192800 Rh2BG204900 Rh2CG198000 Rh2DG200000 Rh6AG483900 Rh6AG484000 Rh6BG492900 Rh6BG493000 Rh6CG497800 Rh6CG497900 Rh6DG484400 Rh6DG484600
rosa_wichuraiana	Rw2G015270 Rw6G042130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	5 cut(s)	524, 923, 1325, 1680, 1735
AatII	GACGTC	5 cut(s)	501, 1476, 1733, 2126, 2201
AccB1I	GGYRCC	2 cut(s)	647, 2305
AccI	GTMKAC	3 cut(s)	92, 1835, 2126
AccIII	TCCGGA	1 cut(s)	1786
AclWI	GGATC	6 cut(s)	1094, 1122, 1493, 1506, 1916, 1929
AcoI	YGGCCR	7 cut(s)	81, 213, 291, 454, 489, 733, 1443
AcsI	RAATTY	2 cut(s)	958, 1591
AcuI	CTGAAG	1 cut(s)	1793
AcvI	CACGTG	2 cut(s)	594, 1918
AfeI	AGCGCT	1 cut(s)	1057
AfiI	CCNNNNNNNGG	8 cut(s)	528, 582, 694, 819, 823, 1254, 1348, 1942
AflIII	ACRYGT	3 cut(s)	1702, 1747, 1915
AgsI	TTSAA	3 cut(s)	616, 1714, 1999
AhdI	GACNNNNNGTC	2 cut(s)	1281, 2209
AjiI	CACGTC	1 cut(s)	1449
AjnI	CCWGG	6 cut(s)	338, 581, 650, 805, 1633, 2025
AleI	CACNNNNGTG	2 cut(s)	1094, 1451
Alw21I	GWGCWC	2 cut(s)	420, 1034
Alw26I	GTCTC	4 cut(s)	175, 286, 461, 1693
AlwI	GGATC	6 cut(s)	1094, 1122, 1493, 1506, 1916, 1929
AlwNI	CAGNNNCTG	1 cut(s)	50
Ama87I	CYCGRG	4 cut(s)	437, 533, 1581, 1942
Aor13HI	TCCGGA	1 cut(s)	1786
Aor51HI	AGCGCT	1 cut(s)	1057
ApaI	GGGCCC	3 cut(s)	689, 1052, 1589
ApeKI	GCWGC	6 cut(s)	62, 143, 146, 359, 1364, 1768
ApoI	RAATTY	2 cut(s)	958, 1591
ArsI	GACNNNNNNTTYG	2 cut(s)	545, 577
Asp700I	GAANNNNTTC	1 cut(s)	2324
AsuC2I	CCSGG	8 cut(s)	381, 780, 1255, 1726, 1799, 1879, 1943, 1944
AsuHPI	GGTGA	7 cut(s)	506, 2113, 2167, 2227, 2269, 2293, 2323
AvaI	CYCGRG	4 cut(s)	437, 533, 1581, 1942
AvaII	GGWCC	5 cut(s)	4, 432, 566, 1673, 2077
BaeGI	GKGCMC	4 cut(s)	576, 689, 1052, 1589
BalI	TGGCCA	2 cut(s)	83, 215
BamHI	GGATCC	2 cut(s)	1498, 1921
BanI	GGYRCC	2 cut(s)	647, 2305
BanII	GRGCYC	6 cut(s)	420, 689, 1052, 1589, 1799, 2235
BauI	CACGAG	2 cut(s)	1027, 2146
BbrPI	CACGTG	2 cut(s)	594, 1918
Bbv12I	GWGCWC	2 cut(s)	420, 1034
BbvI	GCAGC	6 cut(s)	49, 155, 158, 371, 1351, 1755
BccI	CCATC	8 cut(s)	329, 698, 817, 982, 1000, 1264, 1561, 1894
BciT130I	CCWGG	6 cut(s)	340, 583, 652, 807, 1635, 2027
BcnI	CCSGG	8 cut(s)	381, 780, 1255, 1726, 1799, 1879, 1943, 1944
BcoDI	GTCTC	4 cut(s)	175, 286, 461, 1693
BfaI	CTAG	2 cut(s)	1335, 1649
BfmI	CTRYAG	1 cut(s)	141
BfoI	RGCGCY	6 cut(s)	651, 1059, 1209, 1769, 1820, 2309
BlpI	GCTNAGC	1 cut(s)	66
BmcAI	AGTACT	2 cut(s)	387, 513
Bme18I	GGWCC	5 cut(s)	4, 432, 566, 1673, 2077
BmeRI	GACNNNNNGTC	2 cut(s)	1281, 2209
BmeT110I	CYCGRG	4 cut(s)	437, 533, 1581, 1942
BmgBI	CACGTC	1 cut(s)	1449
BmrI	ACTGGG	1 cut(s)	472
BmsI	GCATC	3 cut(s)	253, 1684, 1792
BmuI	ACTGGG	1 cut(s)	472
BoxI	GACNNNNGTC	1 cut(s)	1538
BpmI	CTGGAG	2 cut(s)	1617, 2357
Bpu1102I	GCTNAGC	1 cut(s)	66
BpuEI	CTTGAG	1 cut(s)	392
BpuMI	CCSGG	8 cut(s)	381, 780, 1255, 1726, 1799, 1879, 1943, 1944
Bsa29I	ATCGAT	1 cut(s)	1692
BsaAI	YACGTR	2 cut(s)	594, 1918
BsaWI	WCCGGW	5 cut(s)	557, 1163, 1558, 1786, 2297
BsaXI	ACNNNNNCTCC	4 cut(s)	317, 347, 602, 632
Bsc4I	CCNNNNNNNGG	8 cut(s)	528, 582, 694, 819, 823, 1254, 1348, 1942
Bse118I	RCCGGY	6 cut(s)	491, 521, 1229, 1441, 2170, 2308
Bse1I	ACTGG	6 cut(s)	260, 478, 519, 1116, 1422, 1684
Bse3DI	GCAATG	1 cut(s)	2385
BseAI	TCCGGA	1 cut(s)	1786
BseBI	CCWGG	6 cut(s)	340, 583, 652, 807, 1635, 2027
BseCI	ATCGAT	1 cut(s)	1692
BseGI	GGATG	5 cut(s)	321, 828, 1644, 1783, 1886
BseLI	CCNNNNNNNGG	8 cut(s)	528, 582, 694, 819, 823, 1254, 1348, 1942
BseMI	GCAATG	1 cut(s)	2385
BseMII	CTCAG	5 cut(s)	42, 382, 1014, 2004, 2234
BseNI	ACTGG	6 cut(s)	260, 478, 519, 1116, 1422, 1684
BsePI	GCGCGC	1 cut(s)	832
BseRI	GAGGAG	3 cut(s)	568, 610, 710
BseSI	GKGCMC	4 cut(s)	576, 689, 1052, 1589
BseX3I	CGGCCG	2 cut(s)	291, 489
BseXI	GCAGC	6 cut(s)	49, 155, 158, 371, 1351, 1755
BseYI	CCCAGC	3 cut(s)	682, 861, 1052
BsgI	GTGCAG	1 cut(s)	1929
Bsh1285I	CGRYCG	6 cut(s)	294, 492, 1321, 1512, 1692, 1814
BshNI	GGYRCC	2 cut(s)	647, 2305
BshVI	ATCGAT	1 cut(s)	1692
BsiEI	CGRYCG	6 cut(s)	294, 492, 1321, 1512, 1692, 1814
BsiHKAI	GWGCWC	2 cut(s)	420, 1034
BsiHKCI	CYCGRG	4 cut(s)	437, 533, 1581, 1942
BslFI	GGGAC	7 cut(s)	761, 1269, 1288, 1546, 1741, 1959, 2090
BslI	CCNNNNNNNGG	8 cut(s)	528, 582, 694, 819, 823, 1254, 1348, 1942
BsmAI	GTCTC	4 cut(s)	175, 286, 461, 1693
BsmBI	CGTCTC	2 cut(s)	286, 1693
BsmFI	GGGAC	7 cut(s)	761, 1269, 1288, 1546, 1741, 1959, 2090
BsmI	GAATGC	1 cut(s)	2320
BsoBI	CYCGRG	4 cut(s)	437, 533, 1581, 1942
Bsp120I	GGGCCC	3 cut(s)	685, 1048, 1585
Bsp1286I	GDGCHC	8 cut(s)	420, 576, 689, 1034, 1052, 1589, 1799, 2235
Bsp13I	TCCGGA	1 cut(s)	1786
Bsp1720I	GCTNAGC	1 cut(s)	66
Bsp19I	CCATGG	2 cut(s)	900, 1082
BspCNI	CTCAG	5 cut(s)	43, 381, 1013, 2005, 2235
BspDI	ATCGAT	1 cut(s)	1692
BspEI	TCCGGA	1 cut(s)	1786
BspHI	TCATGA	1 cut(s)	1821
BspMAI	CTGCAG	1 cut(s)	145
BspPI	GGATC	6 cut(s)	1094, 1122, 1493, 1506, 1916, 1929
BspT107I	GGYRCC	2 cut(s)	647, 2305
BsrDI	GCAATG	1 cut(s)	2385
BsrFI	RCCGGY	6 cut(s)	491, 521, 1229, 1441, 2170, 2308
BsrI	ACTGG	6 cut(s)	260, 478, 519, 1116, 1422, 1684
BssAI	RCCGGY	6 cut(s)	491, 521, 1229, 1441, 2170, 2308
BssHII	GCGCGC	1 cut(s)	832
BssNAI	GTATAC	1 cut(s)	1836
BssSI	CACGAG	2 cut(s)	1027, 2146
BssT1I	CCWWGG	2 cut(s)	900, 1082
Bst1107I	GTATAC	1 cut(s)	1836
Bst2BI	CACGAG	2 cut(s)	1027, 2146
Bst2UI	CCWGG	6 cut(s)	340, 583, 652, 807, 1635, 2027
Bst4CI	ACNGT	9 cut(s)	46, 1096, 1907, 1966, 2155, 2194, 2227, 2281, 2324
BstAPI	GCANNNNNTGC	1 cut(s)	1861
BstBAI	YACGTR	2 cut(s)	594, 1918
BstDEI	CTNAG	6 cut(s)	51, 66, 368, 1000, 2013, 2243
BstDSI	CCRYGG	9 cut(s)	325, 736, 817, 900, 1082, 1095, 1321, 1353, 1827
BstF5I	GGATG	5 cut(s)	321, 828, 1644, 1783, 1886
BstH2I	RGCGCY	6 cut(s)	651, 1059, 1209, 1769, 1820, 2309
BstMAI	GTCTC	4 cut(s)	175, 286, 461, 1693
BstMCI	CGRYCG	6 cut(s)	294, 492, 1321, 1512, 1692, 1814
BstNI	CCWGG	6 cut(s)	340, 583, 652, 807, 1635, 2027
BstNSI	RCATGY	2 cut(s)	1751, 2360
BstPAI	GACNNNNGTC	1 cut(s)	1538
BstSFI	CTRYAG	1 cut(s)	141
BstSLI	GKGCMC	4 cut(s)	576, 689, 1052, 1589
BstV1I	GCAGC	6 cut(s)	49, 155, 158, 371, 1351, 1755
BstX2I	RGATCY	2 cut(s)	1498, 1921
BstXI	CCANNNNNNTGG	1 cut(s)	1453
BstYI	RGATCY	2 cut(s)	1498, 1921
BstZ17I	GTATAC	1 cut(s)	1836
BstZI	CGGCCG	2 cut(s)	291, 489
Bsu15I	ATCGAT	1 cut(s)	1692
BsuTUI	ATCGAT	1 cut(s)	1692
BtgI	CCRYGG	9 cut(s)	325, 736, 817, 900, 1082, 1095, 1321, 1353, 1827
BtgZI	GCGATG	2 cut(s)	1001, 1019
BtrI	CACGTC	1 cut(s)	1449
BtsCI	GGATG	5 cut(s)	321, 828, 1644, 1783, 1886
BtsI	GCAGTG	1 cut(s)	138
BtsIMutI	CAGTG	5 cut(s)	51, 138, 253, 1109, 2199
CaiI	CAGNNNCTG	1 cut(s)	50
CciI	TCATGA	1 cut(s)	1821
Cfr10I	RCCGGY	6 cut(s)	491, 521, 1229, 1441, 2170, 2308
Cfr42I	CCGCGG	1 cut(s)	1324
Cfr9I	CCCGGG	1 cut(s)	1942
ClaI	ATCGAT	1 cut(s)	1692
CseI	GACGC	7 cut(s)	351, 355, 514, 1222, 1436, 1710, 1998
CviAII	CATG	8 cut(s)	211, 901, 1083, 1267, 1381, 1748, 1822, 2357
DdeI	CTNAG	6 cut(s)	51, 66, 368, 1000, 2013, 2243
DinI	GGCGCC	2 cut(s)	649, 2307
DrdI	GACNNNNNNGTC	5 cut(s)	524, 923, 1325, 1680, 1735
DriI	GACNNNNNGTC	2 cut(s)	1281, 2209
DseDI	GACNNNNNNGTC	5 cut(s)	524, 923, 1325, 1680, 1735
EaeI	YGGCCR	7 cut(s)	81, 213, 291, 454, 489, 733, 1443
EagI	CGGCCG	2 cut(s)	291, 489
Eam1105I	GACNNNNNGTC	2 cut(s)	1281, 2209
EciI	GGCGGA	1 cut(s)	170
Ecl136II	GAGCTC	1 cut(s)	418
EclXI	CGGCCG	2 cut(s)	291, 489
Eco130I	CCWWGG	2 cut(s)	900, 1082
Eco24I	GRGCYC	6 cut(s)	420, 689, 1052, 1589, 1799, 2235
Eco47I	GGWCC	5 cut(s)	4, 432, 566, 1673, 2077
Eco47III	AGCGCT	1 cut(s)	1057
Eco52I	CGGCCG	2 cut(s)	291, 489
Eco53kI	GAGCTC	1 cut(s)	418
Eco57I	CTGAAG	1 cut(s)	1793
Eco72I	CACGTG	2 cut(s)	594, 1918
Eco88I	CYCGRG	4 cut(s)	437, 533, 1581, 1942
EcoICRI	GAGCTC	1 cut(s)	418
EcoO109I	RGGNCCY	5 cut(s)	686, 1049, 1585, 1673, 2077
EcoRI	GAATTC	1 cut(s)	958
EcoRII	CCWGG	6 cut(s)	338, 581, 650, 805, 1633, 2025
EcoT14I	CCWWGG	2 cut(s)	900, 1082
EcoT22I	ATGCAT	1 cut(s)	1268
EcoT38I	GRGCYC	6 cut(s)	420, 689, 1052, 1589, 1799, 2235
EgeI	GGCGCC	2 cut(s)	649, 2307
EheI	GGCGCC	2 cut(s)	649, 2307
ErhI	CCWWGG	2 cut(s)	900, 1082
Esp3I	CGTCTC	2 cut(s)	286, 1693
FaeI	CATG	8 cut(s)	214, 904, 1086, 1270, 1384, 1751, 1825, 2360
FalI	AAGNNNNNCTT	2 cut(s)	1004, 1036
FaqI	GGGAC	7 cut(s)	761, 1269, 1288, 1546, 1741, 1959, 2090
FatI	CATG	8 cut(s)	210, 900, 1082, 1266, 1380, 1747, 1821, 2356
FauI	CCCGC	9 cut(s)	238, 728, 837, 947, 1039, 1135, 1360, 1645, 2167
FblI	GTMKAC	3 cut(s)	92, 1835, 2126
FokI	GGATG	5 cut(s)	308, 835, 1631, 1770, 1873
FriOI	GRGCYC	6 cut(s)	420, 689, 1052, 1589, 1799, 2235
FspBI	CTAG	2 cut(s)	1335, 1649
GsaI	CCCAGC	3 cut(s)	686, 865, 1056
GsuI	CTGGAG	2 cut(s)	1617, 2357
HaeII	RGCGCY	6 cut(s)	651, 1059, 1209, 1769, 1820, 2309
HgaI	GACGC	7 cut(s)	351, 355, 514, 1222, 1436, 1710, 1998
Hin1II	CATG	8 cut(s)	214, 904, 1086, 1270, 1384, 1751, 1825, 2360
HincII	GTYRAC	3 cut(s)	1330, 1642, 2127
HindII	GTYRAC	3 cut(s)	1330, 1642, 2127
HphI	GGTGA	7 cut(s)	506, 2113, 2167, 2227, 2269, 2293, 2323
Hpy188III	TCNNGA	8 cut(s)	401, 603, 937, 1118, 1480, 1787, 1822, 2329
HpyAV	CCTTC	6 cut(s)	26, 125, 201, 1006, 1291, 1768
HpyCH4III	ACNGT	9 cut(s)	46, 1096, 1907, 1966, 2155, 2194, 2227, 2281, 2324
HpyCH4V	TGCA	4 cut(s)	143, 1266, 1864, 1910
HpyF3I	CTNAG	6 cut(s)	51, 66, 368, 1000, 2013, 2243
Hsp92II	CATG	8 cut(s)	214, 904, 1086, 1270, 1384, 1751, 1825, 2360
KasI	GGCGCC	2 cut(s)	647, 2305
Kpn2I	TCCGGA	1 cut(s)	1786
KroI	GCCGGC	1 cut(s)	2170
KroNI	GCCGGC	1 cut(s)	2172
KspI	CCGCGG	1 cut(s)	1324
Lsp1109I	GCAGC	6 cut(s)	49, 155, 158, 371, 1351, 1755
LweI	GCATC	3 cut(s)	253, 1684, 1792
MaeI	CTAG	2 cut(s)	1335, 1649
MboII	GAAGA	6 cut(s)	47, 101, 1315, 1424, 2230, 2278
MfeI	CAATTG	1 cut(s)	625
MflI	RGATCY	2 cut(s)	1498, 1921
MhlI	GDGCHC	8 cut(s)	420, 576, 689, 1034, 1052, 1589, 1799, 2235
MlsI	TGGCCA	2 cut(s)	83, 215
MluCI	AATT	5 cut(s)	625, 958, 1591, 1999, 2389
MluI	ACGCGT	1 cut(s)	1702
MluNI	TGGCCA	2 cut(s)	83, 215
Mly113I	GGCGCC	2 cut(s)	648, 2306
MmeI	TCCRAC	6 cut(s)	309, 909, 1215, 1522, 2181, 2325
Mox20I	TGGCCA	2 cut(s)	83, 215
Mph1103I	ATGCAT	1 cut(s)	1268
MroI	TCCGGA	1 cut(s)	1786
MroNI	GCCGGC	1 cut(s)	2170
MroXI	GAANNNNTTC	1 cut(s)	2324
MscI	TGGCCA	2 cut(s)	83, 215
MseI	TTAA	2 cut(s)	1022, 2082
MslI	CAYNNNNRTG	3 cut(s)	1094, 1451, 1752
Msp20I	TGGCCA	2 cut(s)	83, 215
MspA1I	CMGCKG	5 cut(s)	62, 149, 764, 1323, 1604
MunI	CAATTG	1 cut(s)	625
Mva1269I	GAATGC	1 cut(s)	2320
MvaI	CCWGG	6 cut(s)	340, 583, 652, 807, 1635, 2027
NaeI	GCCGGC	1 cut(s)	2172
NarI	GGCGCC	2 cut(s)	648, 2306
NciI	CCSGG	8 cut(s)	381, 780, 1255, 1726, 1799, 1879, 1943, 1944
NcoI	CCATGG	2 cut(s)	900, 1082
NgoMIV	GCCGGC	1 cut(s)	2170
NlaIII	CATG	8 cut(s)	214, 904, 1086, 1270, 1384, 1751, 1825, 2360
NmeAIII	GCCGAG	6 cut(s)	269, 432, 467, 927, 1055, 1985
NmuCI	GTSAC	6 cut(s)	46, 494, 974, 2155, 2194, 2281
NsiI	ATGCAT	1 cut(s)	1268
NspI	RCATGY	2 cut(s)	1751, 2360
OliI	CACNNNNGTG	2 cut(s)	1094, 1451
PagI	TCATGA	1 cut(s)	1821
PauI	GCGCGC	1 cut(s)	832
PciI	ACATGT	1 cut(s)	1747
PcsI	WCGNNNNNNNCGW	4 cut(s)	921, 2126, 2135, 2207
PctI	GAATGC	1 cut(s)	2320
PdiI	GCCGGC	1 cut(s)	2172
PdmI	GAANNNNTTC	1 cut(s)	2324
PfeI	GAWTC	9 cut(s)	132, 263, 561, 599, 707, 840, 1017, 1258, 1807
PflFI	GACNNNGTC	1 cut(s)	923
PfoI	TCCNGGA	3 cut(s)	581, 1253, 1724
Ple19I	CGATCG	2 cut(s)	1692, 1814
PluTI	GGCGCC	2 cut(s)	651, 2309
PmaCI	CACGTG	2 cut(s)	594, 1918
PmlI	CACGTG	2 cut(s)	594, 1918
Ppu21I	YACGTR	2 cut(s)	594, 1918
PpuMI	RGGWCCY	2 cut(s)	1673, 2077
PscI	ACATGT	1 cut(s)	1747
PshAI	GACNNNNGTC	1 cut(s)	1538
Psp124BI	GAGCTC	1 cut(s)	420
Psp5II	RGGWCCY	2 cut(s)	1673, 2077
Psp6I	CCWGG	6 cut(s)	338, 581, 650, 805, 1633, 2025
PspCI	CACGTG	2 cut(s)	594, 1918
PspFI	CCCAGC	3 cut(s)	682, 861, 1052
PspGI	CCWGG	6 cut(s)	338, 581, 650, 805, 1633, 2025
PspOMI	GGGCCC	3 cut(s)	685, 1048, 1585
PspPPI	RGGWCCY	2 cut(s)	1673, 2077
PstI	CTGCAG	1 cut(s)	145
PstNI	CAGNNNCTG	1 cut(s)	50
PsuI	RGATCY	2 cut(s)	1498, 1921
PsyI	GACNNNGTC	1 cut(s)	923
PteI	GCGCGC	1 cut(s)	832
PvuI	CGATCG	2 cut(s)	1692, 1814
PvuII	CAGCTG	1 cut(s)	62
RseI	CAYNNNNRTG	3 cut(s)	1094, 1451, 1752
SacI	GAGCTC	1 cut(s)	420
SacII	CCGCGG	1 cut(s)	1324
SalI	GTCGAC	1 cut(s)	2125
SaqAI	TTAA	2 cut(s)	1022, 2082
ScaI	AGTACT	2 cut(s)	387, 513
SduI	GDGCHC	8 cut(s)	420, 576, 689, 1034, 1052, 1589, 1799, 2235
SfaNI	GCATC	3 cut(s)	253, 1684, 1792
SfcI	CTRYAG	1 cut(s)	141
SfoI	GGCGCC	2 cut(s)	649, 2307
Sfr303I	CCGCGG	1 cut(s)	1324
SgrAI	CRCCGGYG	2 cut(s)	521, 2308
SgrBI	CCGCGG	1 cut(s)	1324
SgrDI	CGTCGACG	1 cut(s)	2125
SinI	GGWCC	5 cut(s)	4, 432, 566, 1673, 2077
SmaI	CCCGGG	1 cut(s)	1944
SmiMI	CAYNNNNRTG	3 cut(s)	1094, 1451, 1752
SmlI	CTYRAG	1 cut(s)	407
SmoI	CTYRAG	1 cut(s)	407
Sse9I	AATT	5 cut(s)	625, 958, 1591, 1999, 2389
SspDI	GGCGCC	2 cut(s)	647, 2305
SspI	AATATT	1 cut(s)	1717
SspMI	CTAG	2 cut(s)	1335, 1649
SstI	GAGCTC	1 cut(s)	420
StyI	CCWWGG	2 cut(s)	900, 1082
TaaI	ACNGT	9 cut(s)	46, 1096, 1907, 1966, 2155, 2194, 2227, 2281, 2324
TasI	AATT	5 cut(s)	625, 958, 1591, 1999, 2389
TatI	WGTACW	3 cut(s)	385, 511, 2142
TauI	GCSGC	9 cut(s)	764, 848, 873, 899, 954, 1403, 1571, 1766, 1805
TfiI	GAWTC	9 cut(s)	132, 263, 561, 599, 707, 840, 1017, 1258, 1807
Tru1I	TTAA	2 cut(s)	1022, 2082
Tru9I	TTAA	2 cut(s)	1022, 2082
TscAI	CASTG	5 cut(s)	51, 145, 260, 1116, 2199
TseFI	GTSAC	6 cut(s)	46, 494, 974, 2155, 2194, 2281
TseI	GCWGC	6 cut(s)	62, 143, 146, 359, 1364, 1768
Tsp45I	GTSAC	6 cut(s)	46, 494, 974, 2155, 2194, 2281
TspGWI	ACGGA	3 cut(s)	268, 382, 962
TspMI	CCCGGG	1 cut(s)	1942
TspRI	CASTG	5 cut(s)	51, 145, 260, 1116, 2199
Tth111I	GACNNNGTC	1 cut(s)	923
VpaK11BI	GGWCC	5 cut(s)	4, 432, 566, 1673, 2077
XapI	RAATTY	2 cut(s)	958, 1591
XceI	RCATGY	2 cut(s)	1751, 2360
XmaI	CCCGGG	1 cut(s)	1942
XmiI	GTMKAC	3 cut(s)	92, 1835, 2126
XmnI	GAANNNNTTC	1 cut(s)	2324
XspI	CTAG	2 cut(s)	1335, 1649
ZraI	GACGTC	5 cut(s)	499, 1474, 1731, 2124, 2199
ZrmI	AGTACT	2 cut(s)	387, 513
Zsp2I	ATGCAT	1 cut(s)	1268

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.