Prupe.1G425600_v2.0.a1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001634
Physical Location & Seq
Forward (+)
36631469 .. 36633192
1724 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G425600.1

Sequence Viewer

Length: 1713 bp
ATGCGAACCTATCAATGCCATTGGCACTGCAAATACTGCCGTTCCCAAAGTAGCAAAAGGAAAAATTCAGAAGCTCAGGCTTGCAACGATTGGGAAGATGAAAACATCCATTTGCATGCTTTCACTAACGCTGATGCTGATGCTTCTGGTTCTCTCTCACAACATCTTGTGCACGGTAGCGGTAGCAGGAGAGAGAGAGAGCAGCAGATTAAAAATACTTACATCATTCAGATGGACAAGTCCAAAATGCCTGCAAGTTTTGTCGACGACCATTTCCAATGGTATGGCTCCTCTTTGAAATCGGTGTCCAACTCAGCAGACATGCTCTACACCTACAAAAATGTTATCCACGGCTTCTCCACAAGGCTAACTGCTGAGGAAGTTGAGCTTCTTGAAAAACAATCCGGAATTCTTTCTGTTATGCCTGAGTTGAGATATGAGGTCCACACAACTCGGACGCCCCAGTTCCTTGGAATGTTAGGCTGCATAAACGAAGCCGTCTTCCCGGCATCAGAGAAACTCAGCAAGGTGATTATTGGAGTGGTTGATACTGGCATCAAAAGCTACGATGACAAAGGGCTTGGGCCAGTGCCAAGAAGCTGGAGAGGACTGTGTGAGGAAGGCAAGAACTTCAACTCCTCAAGCTGCAACCGAAAACTCATTGGTGCGAGGTTTTTCCCAAAAGGGTATGAAGCATCAAATCTTGGACCCATTGATGAAAAAGTAGAATCAAGATCACCAAGAGATGTTGATGGCCATAGAACTCACGCCTCAACCATTGCAGCCGGATCTGCCGTTCCAGGAGCTAGCCTATTTGGTTACGCTTCAGGGACAGCAAGGGGGATTGCTACACAAGCTAGAGTGGCCACATATAAGGCTTGCTGGTCTGGTTGGTGCTTGAGCTCTGATATTCTAGCCGCAATGGACAAAGCTGTTGAAGATGGTGTCCACATTTTATCTGTGTCTATTGGGAGATCGCAGTATGAGGATTTCTACACCGACTTTATTGCTATTGGAGCATTCTCTGCCATGGCCAAGGGAGTCTTTGTATCGTGTTCAGCTGGAAATCGTGGGCCAGAAGCGGATAGCACTTCTAACAACGCACCTTGGATAACCACCGTGGGTGCTGGAACAATAGACCGTGATTTCCCCGCTCATGTTAGCTTGGGAAATGGGAAAAAATACAGAGGAGCATCAATTTACAGCGGAACACACTTATCTAGTGGATTGCATCCGCTTGTTTATGCTCGCAATGCAAGTAACTCCACAAGTGACTCCACAAGTGATCCATGCGCTCCTGACAGCTTGATTCCTGAAAAGGTTTTTGGGAAAATTGTGGTGTGTGATCAGGGAGGAACTTACAGCAGGGTCGATAAGAGTATGGTGGTCAAAAAGGCCGGTGGCATGGGGATGATTTTGGCTGACATTGAAGGTTATGATGAAGAACAACTTGTTGTCGACTCATTTGTCTTGCCTGTAGTCGTGGTTGGCCAAAAAGCAGGTGATGCAATAAAAAGATATATTGCCTCCCACGACAATCCAAAAGCCACATTTTCTGGCGGGAAGACAGAGTTGGGTGTCGAACCCTCACCGGTGGTGGCAGCATTCAGCTCTCGAGGACCAAATCCAATCTGTGTAGAACTACTCAAACCAGACCTACTAGCACCTGGAGTGATTATGGAGTGGTGCAGTTGGACCAAGTGGACTCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

571

Amino Acids

61.81

Weight (kDa)

6.76

Isoelectric Point (pI)

35.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1490
AasI GACNNNNNNGTC 1 cut(s) 1466
Acc36I ACCTGC 1 cut(s) 1490
AccBSI CCGCTC 1 cut(s) 1154
AccI GTMKAC 2 cut(s) 264, 1458
AccIII TCCGGA 1 cut(s) 404
AciI CCGC 7 cut(s) 180, 918, 1082, 1152, 1206, 1235, 1560
AclWI GGATC 2 cut(s) 796, 1280
AcoI YGGCCR 4 cut(s) 754, 864, 1032, 1489
AcsI RAATTY 2 cut(s) 64, 408
AcuI CTGAAG 1 cut(s) 810
AcyI GRCGYC 1 cut(s) 458
AgeI ACCGGT 1 cut(s) 1591
AgsI TTSAA 5 cut(s) 298, 395, 634, 938, 1430
AjnI CCWGG 2 cut(s) 799, 1666
AjuI GAANNNNNNNTTGG 4 cut(s) 1308, 1340, 1556, 1588
AloI GAACNNNNNNTCC 4 cut(s) 620, 652, 780, 812
Alw21I GWGCWC 2 cut(s) 174, 905
Alw44I GTGCAC 1 cut(s) 170
AlwI GGATC 2 cut(s) 796, 1280
Ama87I CYCGRG 1 cut(s) 1614
Aor13HI TCCGGA 1 cut(s) 404
AoxI GGCC 7 cut(s) 584, 754, 864, 1032, 1073, 1395, 1489
ApaLI GTGCAC 1 cut(s) 170
ApeKI GCWGC 5 cut(s) 202, 483, 645, 782, 1601
ApoI RAATTY 2 cut(s) 64, 408
AsiGI ACCGGT 1 cut(s) 1591
Asp700I GAANNNNTTC 1 cut(s) 412
AspLEI GCGC 1 cut(s) 1295
AspS9I GGNCC 6 cut(s) 442, 584, 707, 1073, 1619, 1695
AsuC2I CCSGG 1 cut(s) 506
AsuHPI GGTGA 4 cut(s) 541, 729, 1514, 1581
AsuNHI GCTAGC 1 cut(s) 806
AvaI CYCGRG 1 cut(s) 1614
AvaII GGWCC 4 cut(s) 442, 707, 1619, 1695
BaeGI GKGCMC 1 cut(s) 174
BalI TGGCCA 4 cut(s) 756, 866, 1034, 1491
BanII GRGCYC 1 cut(s) 905
BbsI GAAGAC 2 cut(s) 493, 1571
Bbv12I GWGCWC 2 cut(s) 174, 905
BbvCI CCTCAGC 1 cut(s) 375
BbvI GCAGC 5 cut(s) 214, 470, 632, 794, 1613
BccI CCATC 3 cut(s) 226, 746, 935
BceAI ACGGC 4 cut(s) 24, 367, 482, 779
BcgI CGANNNNNNTGC 2 cut(s) 989, 1023
BciT130I CCWGG 2 cut(s) 801, 1668
BclI TGATCA 1 cut(s) 1345
BcnI CCSGG 1 cut(s) 506
BfaI CTAG 5 cut(s) 807, 858, 914, 1221, 1661
BfmI CTRYAG 1 cut(s) 1476
BfuAI ACCTGC 1 cut(s) 1490
BisI GCNGC 6 cut(s) 203, 484, 646, 783, 918, 1602
BlsI GCNGC 6 cut(s) 204, 485, 647, 784, 919, 1603
Bme1390I CCNGG 3 cut(s) 506, 801, 1668
Bme18I GGWCC 4 cut(s) 442, 707, 1619, 1695
BmeT110I CYCGRG 1 cut(s) 1614
BmgT120I GGNCC 6 cut(s) 442, 584, 707, 1073, 1619, 1695
BmiI GGNNCC 2 cut(s) 289, 709
BmrFI CCNGG 3 cut(s) 506, 801, 1668
BmrI ACTGGG 1 cut(s) 457
BmsI GCATC 8 cut(s) 124, 130, 518, 564, 704, 1202, 1240, 1495
BmtI GCTAGC 1 cut(s) 810
BmuI ACTGGG 1 cut(s) 457
BpiI GAAGAC 2 cut(s) 493, 1571
BpmI CTGGAG 2 cut(s) 622, 1689
Bpu10I CCTNAGC 2 cut(s) 75, 375
BpuEI CTTGAG 2 cut(s) 625, 919
BpuMI CCSGG 1 cut(s) 506
BsaHI GRCGYC 1 cut(s) 458
BsaJI CCNNGG 6 cut(s) 349, 469, 1029, 1035, 1106, 1119
BsaWI WCCGGW 2 cut(s) 404, 1591
BsaXI ACNNNNNCTCC 6 cut(s) 341, 371, 620, 650, 1032, 1062
Bse118I RCCGGY 2 cut(s) 1397, 1591
Bse1I ACTGG 3 cut(s) 463, 556, 587
Bse3DI GCAATG 3 cut(s) 777, 927, 1258
BseAI TCCGGA 1 cut(s) 404
BseBI CCWGG 2 cut(s) 801, 1668
BseDI CCNNGG 6 cut(s) 349, 469, 1029, 1035, 1106, 1119
BseGI GGATG 3 cut(s) 105, 1231, 1416
BseMI GCAATG 3 cut(s) 777, 927, 1258
BseMII CTCAG 5 cut(s) 89, 327, 366, 417, 535
BseNI ACTGG 3 cut(s) 463, 556, 587
BseRI GAGGAG 3 cut(s) 280, 628, 1203
BseSI GKGCMC 1 cut(s) 174
BseXI GCAGC 5 cut(s) 214, 470, 632, 794, 1613
BsgI GTGCAG 1 cut(s) 1708
BshFI GGCC 7 cut(s) 586, 756, 866, 1034, 1075, 1397, 1491
BshTI ACCGGT 1 cut(s) 1591
BsiHKAI GWGCWC 2 cut(s) 174, 905
BsiHKCI CYCGRG 1 cut(s) 1614
BsiSI CCGG 5 cut(s) 405, 506, 786, 1398, 1592
BslFI GGGAC 1 cut(s) 844
BsmFI GGGAC 1 cut(s) 844
BsmI GAATGC 2 cut(s) 1019, 1604
BsnI GGCC 7 cut(s) 586, 756, 866, 1034, 1075, 1397, 1491
BsoBI CYCGRG 1 cut(s) 1614
Bsp1286I GDGCHC 2 cut(s) 174, 905
Bsp13I TCCGGA 1 cut(s) 404
Bsp143I GATC 5 cut(s) 734, 788, 974, 1285, 1345
Bsp19I CCATGG 1 cut(s) 1029
BspACI CCGC 7 cut(s) 180, 918, 1082, 1152, 1206, 1235, 1560
BspANI GGCC 7 cut(s) 586, 756, 866, 1034, 1075, 1397, 1491
BspCNI CTCAG 5 cut(s) 88, 326, 367, 418, 534
BspEI TCCGGA 1 cut(s) 404
BspLI GGNNCC 2 cut(s) 289, 709
BspMI ACCTGC 1 cut(s) 1490
BspOI GCTAGC 1 cut(s) 810
BspPI GGATC 2 cut(s) 796, 1280
BsrBI CCGCTC 1 cut(s) 1154
BsrDI GCAATG 3 cut(s) 777, 927, 1258
BsrFI RCCGGY 2 cut(s) 1397, 1591
BsrI ACTGG 3 cut(s) 463, 556, 587
BssAI RCCGGY 2 cut(s) 1397, 1591
BssECI CCNNGG 6 cut(s) 349, 469, 1029, 1035, 1106, 1119
BssMI GATC 5 cut(s) 734, 788, 974, 1285, 1345
BssNI GRCGYC 1 cut(s) 458
BssT1I CCWWGG 4 cut(s) 469, 1029, 1035, 1106
Bst2UI CCWGG 2 cut(s) 801, 1668
Bst4CI ACNGT 4 cut(s) 176, 612, 1120, 1142
BstACI GRCGYC 1 cut(s) 458
BstAPI GCANNNNNTGC 3 cut(s) 36, 1025, 1505
BstC8I GCNNGC 6 cut(s) 82, 117, 252, 808, 880, 1249
BstDEI CTNAG 5 cut(s) 75, 313, 375, 426, 521
BstDSI CCRYGG 3 cut(s) 349, 1029, 1119
BstF5I GGATG 3 cut(s) 105, 1231, 1416
BstHHI GCGC 1 cut(s) 1295
BstKTI GATC 5 cut(s) 737, 791, 977, 1288, 1348
BstMBI GATC 5 cut(s) 734, 788, 974, 1285, 1345
BstMWI GCNNNNNNNGC 9 cut(s) 36, 561, 791, 854, 863, 1016, 1025, 1253, 1505
BstNI CCWGG 2 cut(s) 801, 1668
BstNSI RCATGY 2 cut(s) 119, 325
BstSCI CCNGG 3 cut(s) 504, 799, 1666
BstSFI CTRYAG 1 cut(s) 1476
BstSLI GKGCMC 1 cut(s) 174
BstV1I GCAGC 5 cut(s) 214, 470, 632, 794, 1613
BstV2I GAAGAC 2 cut(s) 493, 1571
BstX2I RGATCY 1 cut(s) 788
BstXI CCANNNNNNTGG 3 cut(s) 284, 470, 600
BstYI RGATCY 1 cut(s) 788
BsuRI GGCC 7 cut(s) 586, 756, 866, 1034, 1075, 1397, 1491
BtgI CCRYGG 3 cut(s) 349, 1029, 1119
BtsCI GGATG 3 cut(s) 105, 1231, 1416
BtsI GCAGTG 1 cut(s) 25
BtsIMutI CAGTG 2 cut(s) 25, 594
BveI ACCTGC 1 cut(s) 1490
Cac8I GCNNGC 6 cut(s) 82, 117, 252, 808, 880, 1249
CfoI GCGC 1 cut(s) 1295
Cfr10I RCCGGY 2 cut(s) 1397, 1591
Cfr13I GGNCC 6 cut(s) 442, 584, 707, 1073, 1619, 1695
CseI GACGC 1 cut(s) 466
CspAI ACCGGT 1 cut(s) 1591
CviAII CATG 6 cut(s) 116, 322, 1030, 1157, 1290, 1405
DdeI CTNAG 5 cut(s) 75, 313, 375, 426, 521
DpnI GATC 5 cut(s) 736, 790, 976, 1287, 1347
DpnII GATC 5 cut(s) 734, 788, 974, 1285, 1345
DrdI GACNNNNNNGTC 1 cut(s) 1466
DseDI GACNNNNNNGTC 1 cut(s) 1466
EaeI YGGCCR 4 cut(s) 754, 864, 1032, 1489
Ecl136II GAGCTC 1 cut(s) 903
Eco130I CCWWGG 4 cut(s) 469, 1029, 1035, 1106
Eco24I GRGCYC 1 cut(s) 905
Eco47I GGWCC 4 cut(s) 442, 707, 1619, 1695
Eco53kI GAGCTC 1 cut(s) 903
Eco57I CTGAAG 1 cut(s) 810
Eco88I CYCGRG 1 cut(s) 1614
EcoICRI GAGCTC 1 cut(s) 903
EcoRI GAATTC 1 cut(s) 408
EcoRII CCWGG 2 cut(s) 799, 1666
EcoT14I CCWWGG 4 cut(s) 469, 1029, 1035, 1106
EcoT38I GRGCYC 1 cut(s) 905
ErhI CCWWGG 4 cut(s) 469, 1029, 1035, 1106
FaeI CATG 6 cut(s) 119, 325, 1033, 1160, 1293, 1408
FalI AAGNNNNNCTT 6 cut(s) 372, 404, 1028, 1060, 1434, 1466
FaqI GGGAC 1 cut(s) 844
FatI CATG 6 cut(s) 115, 321, 1029, 1156, 1289, 1404
FauI CCCGC 2 cut(s) 1159, 1553
FbaI TGATCA 1 cut(s) 1345
FblI GTMKAC 2 cut(s) 264, 1458
Fnu4HI GCNGC 6 cut(s) 203, 484, 646, 783, 918, 1602
FokI GGATG 3 cut(s) 92, 1218, 1423
FriOI GRGCYC 1 cut(s) 905
Fsp4HI GCNGC 6 cut(s) 203, 484, 646, 783, 918, 1602
FspBI CTAG 5 cut(s) 807, 858, 914, 1221, 1661
GlaI GCGC 1 cut(s) 1294
GluI GCNGC 6 cut(s) 203, 484, 646, 783, 918, 1602
GsuI CTGGAG 2 cut(s) 622, 1689
HaeIII GGCC 7 cut(s) 586, 756, 866, 1034, 1075, 1397, 1491
HapII CCGG 5 cut(s) 405, 506, 786, 1398, 1592
HgaI GACGC 1 cut(s) 466
HhaI GCGC 1 cut(s) 1295
Hin1I GRCGYC 1 cut(s) 458
Hin1II CATG 6 cut(s) 119, 325, 1033, 1160, 1293, 1408
Hin6I GCGC 1 cut(s) 1293
HinP1I GCGC 1 cut(s) 1293
HincII GTYRAC 2 cut(s) 265, 1459
HindII GTYRAC 2 cut(s) 265, 1459
HinfI GANTC 6 cut(s) 728, 1041, 1274, 1309, 1460, 1705
HpaII CCGG 5 cut(s) 405, 506, 786, 1398, 1592
HphI GGTGA 4 cut(s) 541, 729, 1514, 1581
Hpy166II GTNNAC 6 cut(s) 172, 265, 445, 949, 1459, 1704
Hpy188I TCNGA 5 cut(s) 70, 231, 456, 514, 907
Hpy188III TCNNGA 6 cut(s) 392, 405, 732, 1298, 1313, 1614
Hpy8I GTNNAC 6 cut(s) 172, 265, 445, 949, 1459, 1704
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 2 cut(s) 614, 1424
HpyCH4III ACNGT 4 cut(s) 176, 612, 1120, 1142
HpyF10VI GCNNNNNNNGC 9 cut(s) 36, 561, 791, 854, 863, 1016, 1025, 1253, 1505
HpyF3I CTNAG 5 cut(s) 75, 313, 375, 426, 521
Hsp92I GRCGYC 1 cut(s) 458
Hsp92II CATG 6 cut(s) 119, 325, 1033, 1160, 1293, 1408
HspAI GCGC 1 cut(s) 1293
Kpn2I TCCGGA 1 cut(s) 404
Ksp22I TGATCA 1 cut(s) 1345
Kzo9I GATC 5 cut(s) 734, 788, 974, 1285, 1345
LmnI GCTCC 5 cut(s) 293, 803, 1016, 1190, 1300
Lsp1109I GCAGC 5 cut(s) 214, 470, 632, 794, 1613
LweI GCATC 8 cut(s) 124, 130, 518, 564, 704, 1202, 1240, 1495
MaeI CTAG 5 cut(s) 807, 858, 914, 1221, 1661
MaeIII GTNAC 3 cut(s) 818, 1259, 1271
MalI GATC 5 cut(s) 736, 790, 976, 1287, 1347
MbiI CCGCTC 1 cut(s) 1154
MboI GATC 5 cut(s) 734, 788, 974, 1285, 1345
MboII GAAGA 5 cut(s) 107, 493, 950, 1454, 1576
MflI RGATCY 1 cut(s) 788
MhlI GDGCHC 2 cut(s) 174, 905
MlsI TGGCCA 4 cut(s) 756, 866, 1034, 1491
MluCI AATT 4 cut(s) 64, 408, 1197, 1332
MluNI TGGCCA 4 cut(s) 756, 866, 1034, 1491
MlyI GAGTC 4 cut(s) 1050, 1268, 1454, 1699
MmeI TCCRAC 2 cut(s) 333, 1673
Mox20I TGGCCA 4 cut(s) 756, 866, 1034, 1491
MroI TCCGGA 1 cut(s) 404
MroXI GAANNNNTTC 1 cut(s) 412
MscI TGGCCA 4 cut(s) 756, 866, 1034, 1491
MseI TTAA 1 cut(s) 210
MslI CAYNNNNRTG 3 cut(s) 114, 230, 1409
Msp20I TGGCCA 4 cut(s) 756, 866, 1034, 1491
MspA1I CMGCKG 2 cut(s) 1061, 1206
MspI CCGG 5 cut(s) 405, 506, 786, 1398, 1592
MspR9I CCNGG 3 cut(s) 506, 801, 1668
Mva1269I GAATGC 2 cut(s) 1019, 1604
MvaI CCWGG 2 cut(s) 801, 1668
MwoI GCNNNNNNNGC 9 cut(s) 36, 561, 791, 854, 863, 1016, 1025, 1253, 1505
NciI CCSGG 1 cut(s) 506
NcoI CCATGG 1 cut(s) 1029
NdeII GATC 5 cut(s) 734, 788, 974, 1285, 1345
NheI GCTAGC 1 cut(s) 806
NlaIII CATG 6 cut(s) 119, 325, 1033, 1160, 1293, 1408
NlaIV GGNNCC 2 cut(s) 289, 709
NmuCI GTSAC 1 cut(s) 1271
NspI RCATGY 2 cut(s) 119, 325
PaeI GCATGC 1 cut(s) 119
PaeR7I CTCGAG 1 cut(s) 1614
PaqCI CACCTGC 1 cut(s) 1490
PctI GAATGC 2 cut(s) 1019, 1604
PdmI GAANNNNTTC 1 cut(s) 412
PfeI GAWTC 2 cut(s) 728, 1309
PfoI TCCNGGA 1 cut(s) 799
PinAI ACCGGT 1 cut(s) 1591
PkrI GCNGC 6 cut(s) 204, 485, 647, 784, 919, 1603
PleI GAGTC 4 cut(s) 1049, 1268, 1454, 1699
PpsI GAGTC 4 cut(s) 1049, 1268, 1454, 1699
Psp124BI GAGCTC 1 cut(s) 905
Psp6I CCWGG 2 cut(s) 799, 1666
PspGI CCWGG 2 cut(s) 799, 1666
PspN4I GGNNCC 2 cut(s) 289, 709
PspPI GGNCC 6 cut(s) 442, 584, 707, 1073, 1619, 1695
PsuI RGATCY 1 cut(s) 788
PvuII CAGCTG 1 cut(s) 1061
RseI CAYNNNNRTG 3 cut(s) 114, 230, 1409
SacI GAGCTC 1 cut(s) 905
SalI GTCGAC 2 cut(s) 263, 1457
SaqAI TTAA 1 cut(s) 210
SatI GCNGC 6 cut(s) 203, 484, 646, 783, 918, 1602
Sau3AI GATC 5 cut(s) 734, 788, 974, 1285, 1345
Sau96I GGNCC 6 cut(s) 442, 584, 707, 1073, 1619, 1695
SchI GAGTC 4 cut(s) 1050, 1268, 1454, 1699
ScrFI CCNGG 3 cut(s) 506, 801, 1668
SduI GDGCHC 2 cut(s) 174, 905
SfaNI GCATC 8 cut(s) 124, 130, 518, 564, 704, 1202, 1240, 1495
SfcI CTRYAG 1 cut(s) 1476
Sfr274I CTCGAG 1 cut(s) 1614
SgrAI CRCCGGYG 1 cut(s) 1591
SinI GGWCC 4 cut(s) 442, 707, 1619, 1695
SlaI CTCGAG 1 cut(s) 1614
SmiMI CAYNNNNRTG 3 cut(s) 114, 230, 1409
SmlI CTYRAG 3 cut(s) 640, 898, 1614
SmoI CTYRAG 3 cut(s) 640, 898, 1614
SphI GCATGC 1 cut(s) 119
Sse9I AATT 4 cut(s) 64, 408, 1197, 1332
SsiI CCGC 7 cut(s) 180, 918, 1082, 1152, 1206, 1235, 1560
SspMI CTAG 5 cut(s) 807, 858, 914, 1221, 1661
SstI GAGCTC 1 cut(s) 905
StyD4I CCNGG 3 cut(s) 504, 799, 1666
StyI CCWWGG 4 cut(s) 469, 1029, 1035, 1106
TaaI ACNGT 4 cut(s) 176, 612, 1120, 1142
TaqI TCGA 5 cut(s) 264, 1371, 1458, 1581, 1615
TasI AATT 4 cut(s) 64, 408, 1197, 1332
TauI GCSGC 1 cut(s) 920
TfiI GAWTC 2 cut(s) 728, 1309
Tru1I TTAA 1 cut(s) 210
Tru9I TTAA 1 cut(s) 210
TscAI CASTG 2 cut(s) 32, 594
TseFI GTSAC 1 cut(s) 1271
TseI GCWGC 5 cut(s) 202, 483, 645, 782, 1601
Tsp45I GTSAC 1 cut(s) 1271
TspDTI ATGAA 4 cut(s) 114, 705, 732, 1455
TspRI CASTG 2 cut(s) 32, 594
VneI GTGCAC 1 cut(s) 170
VpaK11BI GGWCC 4 cut(s) 442, 707, 1619, 1695
XapI RAATTY 2 cut(s) 64, 408
XceI RCATGY 2 cut(s) 119, 325
XhoI CTCGAG 1 cut(s) 1614
XmiI GTMKAC 2 cut(s) 264, 1458
XmnI GAANNNNTTC 1 cut(s) 412
XspI CTAG 5 cut(s) 807, 858, 914, 1221, 1661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.