MD08G1088000.v1.1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001634
Physical Location & Seq
Forward (+)
7271687 .. 7273988
2302 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1088000.v1.1.491

Sequence Viewer

Length: 2259 bp
ATGAGAATGTCGGTTTTCATACTTCCCGTGGCACTTGCGCTTCTGCTGCTCTCTAACATCTCCGCAATTGCAGAAGACGAGAAAGAGCAGCAAATGAAGAAGACGTACATCATTCACATGGACAAGTCCAATATGCCAGCAAGTTTTGAAGATGACCATTTCCGATGGTATGGCTCGTCTTTGAAGCTGGTGTCGGATTCAGCAGACATGCTTTACACATACACAAACGTAATCCACGGCTTTTCCGCGAGGCTGACTCTTGCAGAAGCTGTGCTGCTTGAGAAACAACCAGGAACTGTTTCTGTCCTGCCTGAATTGAGATATGAACTTCAAACAACTCGGACGCCTGAGTTCCTTGGAATATCAGGAAAACATGCAGCTGTCTTCCCTGGATCGGTTAAAGTGAGTGATGTGGTTATTGGAGTTGTAGATTCAGGTGTGTGGCCTGAGAGCAAAAGCTACGATGACAAAAGTCTCGGCCCTGTGCCGAGAAGCTGGAAAGGAAAGTGCGAGGAGGGAAAGAACTTCAACTCCTCAAGCTGCAACCGAAAACTCATTGGTGCGAGGTTTTTCTCAAGGGGGTATGAAGCAGGACTTGGACATCCCATGAATGAAACCAAAGAATCAAGATCACCAAGAGATGATGATGGTCATGGAACTCACACTTCGACCACTGCAGCCTGGTCCCCTGTTCCAGGAGCTAGCCTCTTCGGCTATGCTTCAGGGACAGCAAAGGGAATGGCAACGCAAGCTCGAGTAGCCACATACAAGGTTTGCTGGCTTAGTGGGTGCTTTAGCTCCGATATATTGGCCGCAATGGACAAGGCTGTTGAAGATGGCATTATTACAGACGGTATTGACATTGGAGCTTTCTCTGTTGTGGCGAAGGGAATCTTCGTGTCATGTGCAGCCGGAAATCAGGGGCCATTTAAGGTCAGCTCGAGCAATAATGCTCCTTGGATAACCACTGTCGGTGCTGGAACATTAGACCGGGATTTCCCTGCATATGTTAGCCTTGGAAATCGAAGAAAATACAGAGGTATATCAGTCTACGGCGGAACATCCTTACCTAGTGGATTACTTCCACTTATTTATGGAAGCAATGGAAGCAATCTATGCATTCCTGACAGTTTAGTTCCTGCAAAAGTTGCTGGGAAAATTGTGGTATGTGATCAAGGGGAAAACCCTGAGGTTGAAAAGTGTGCGGTGGTCAAAAGGGCTGGTGGTGTAGGGATGATTTTGGCGGATGCTGAAATTAACGGGGAAGAACTAATTGCCGACGTGCATCTACTGGCTACAATAATGGTTGGCCATAAAGCTGGTAAGGCAATAAAAAGATATATTGCCTCCCAGGCAGATCCAAAAGCGCACTTTGCTTTCGGGAAGACACAGTTAGGCGTCGAACCCTCACCAGTGGTGGCAGCATTCAGCTCTAGAGGACCAAATCCAGTCTCTCCGAGCGTACTCAAACCAGACCTGATAGCACCGGGAGTGAACATCCTAGCTGGGTGGACTGGTGCAGTTGGACCAACAAGACTAGATGACGACACCATGCGGGTGAGCTTTAACATCCTCTCAGGTACATCCATGTCGTGCCCACATGTGAGCGGGTTGGCCGCCATTCTCAAGGCTGCTCACCCAAAATGGAGCCCTGCAGCCATTAAGTCTGCTCTCATGACCACATCCTATACAACATACAAAAATGGAAAAACAATCAAAGATATTGCTACTGGAAATGCTGCAACACCATACGATTACGGTGCTGGGCATGTAGACCCCGTGGCAGCCATTGACCCTGGCCTTGTTTACGATCTTGCAGTCAAGGACTACCTAAGCTTCCTCTGTGCCTTGCATTATACAACAACAGACATCAAGAAATTGACTCACAGAGACTTCACCTGCGATTCAAGCAAGAATTACAGCGTTGGAGATCTTAATTATCCATCTTTTTCTGTAGACCTATACACAAATTCAAACAACGGTGGAGCAGGTACCGAAAAATACACGAGGACTCTGACCAATGTTGGTCCACCAGCAAGATACAATGTCTCTGTGTCCATAGACTCTACACTTGCAGCGTCAGTGAAGATCTTGGTTGAACCAAAATCACTGAGTTTCAGTCAAGCATATGAGAAGAAGACTTATACTGTGACTTTTGTAACTAGTTCCATGCCATCTGGTACAGACATATTTGCTCGCCTGGTCTGGTCTGATGGGAAACACATAGTGAGTAGTCCCATTGCTATCAGCTGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

753

Amino Acids

80.2

Weight (kDa)

8.11

Isoelectric Point (pI)

31.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 35 - 111 2e-14 Peptidase inhibitor I9
Peptidase_S8 PF00082 137 - 573 8.4e-33 Subtilase family
PA PF02225 362 - 444 4.2e-10 PA domain
fn3_6 PF17766 644 - 749 2e-23 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1907
Acc36I ACCTGC 2 cut(s) 1907, 1979
Acc65I GGTACC 1 cut(s) 1991
AccB1I GGYRCC 1 cut(s) 1991
AccBSI CCGCTC 1 cut(s) 1608
AccI GTMKAC 3 cut(s) 1050, 1773, 1956
AccII CGCG 1 cut(s) 248
AciI CCGC 9 cut(s) 63, 246, 813, 1056, 1205, 1244, 1555, 1608, 1617
AclWI GGATC 2 cut(s) 400, 1352
AcoI YGGCCR 3 cut(s) 810, 1309, 1614
AcsI RAATTY 1 cut(s) 1969
AcuI CTGAAG 1 cut(s) 705
AcyI GRCGYC 2 cut(s) 344, 1398
AdeI CACNNNGTG 1 cut(s) 2227
AfaI GTAC 5 cut(s) 107, 1464, 1582, 1993, 2182
AfiI CCNNNNNNNGG 2 cut(s) 394, 695
AflIII ACRYGT 1 cut(s) 1600
AgsI TTSAA 9 cut(s) 149, 184, 332, 529, 833, 1196, 1908, 1974, 2099
AhlI ACTAGT 1 cut(s) 2162
AjiI CACGTC 1 cut(s) 1282
AjnI CCWGG 7 cut(s) 289, 388, 680, 694, 1350, 1795, 2199
AjuI GAANNNNNNNTTGG 2 cut(s) 579, 611
AloI GAACNNNNNNTCC 2 cut(s) 515, 547
Alw26I GTCTC 4 cut(s) 479, 1456, 1884, 2053
AlwI GGATC 2 cut(s) 400, 1352
AlwNI CAGNNNCTG 2 cut(s) 269, 296
Ama87I CYCGRG 2 cut(s) 753, 940
AoxI GGCC 7 cut(s) 443, 478, 810, 923, 1309, 1614, 1798
ApoI RAATTY 1 cut(s) 1969
Asp700I GAANNNNTTC 1 cut(s) 298
Asp718I GGTACC 1 cut(s) 1991
AspLEI GCGC 2 cut(s) 40, 1369
AspS9I GGNCC 6 cut(s) 479, 684, 923, 1439, 1526, 2027
AsuC2I CCSGG 2 cut(s) 992, 1488
AsuHPI GGTGA 5 cut(s) 624, 1401, 1570, 1628, 1888
AsuNHI GCTAGC 1 cut(s) 701
AvaI CYCGRG 2 cut(s) 753, 940
AvaII GGWCC 4 cut(s) 684, 1439, 1526, 2027
AxyI CCTNAGG 1 cut(s) 1188
BaeGI GKGCMC 1 cut(s) 1598
BalI TGGCCA 1 cut(s) 1311
BanI GGYRCC 1 cut(s) 1991
BanII GRGCYC 1 cut(s) 1652
BarI GAAGNNNNNNTAC 2 cut(s) 89, 121
BauI CACGAG 1 cut(s) 2005
BbsI GAAGAC 5 cut(s) 81, 107, 376, 1391, 2144
BccI CCATC 6 cut(s) 159, 641, 830, 1951, 2182, 2207
BceAI ACGGC 2 cut(s) 253, 1069
BcgI CGANNNNNNTGC 2 cut(s) 1742, 1776
BciT130I CCWGG 7 cut(s) 291, 390, 682, 696, 1352, 1797, 2201
BclI TGATCA 1 cut(s) 1171
BcnI CCSGG 2 cut(s) 992, 1488
BcoDI GTCTC 4 cut(s) 479, 1456, 1884, 2053
BcuI ACTAGT 1 cut(s) 2162
BfaI CTAG 6 cut(s) 702, 1071, 1434, 1502, 1538, 2163
BfmI CTRYAG 3 cut(s) 675, 1653, 1953
BfuAI ACCTGC 2 cut(s) 1907, 1979
BglI GCCNNNNNGGC 2 cut(s) 711, 1352
BglII AGATCT 2 cut(s) 1930, 2088
Bme1390I CCNGG 9 cut(s) 291, 390, 682, 696, 992, 1352, 1488, 1797, 2201
Bme18I GGWCC 4 cut(s) 684, 1439, 1526, 2027
BmeT110I CYCGRG 2 cut(s) 753, 940
BmgBI CACGTC 1 cut(s) 1282
BmgT120I GGNCC 6 cut(s) 479, 684, 923, 1439, 1526, 2027
BmiI GGNNCC 4 cut(s) 686, 924, 1649, 1993
BmrFI CCNGG 9 cut(s) 291, 390, 682, 696, 992, 1352, 1488, 1797, 2201
BmsI GCATC 2 cut(s) 1237, 1294
BmtI GCTAGC 1 cut(s) 705
BoxI GACNNNNGTC 1 cut(s) 471
BpiI GAAGAC 5 cut(s) 81, 107, 376, 1391, 2144
BplI GAGNNNNNCTC 4 cut(s) 241, 273, 690, 722
Bpu10I CCTNAGC 1 cut(s) 1832
BpuEI CTTGAG 4 cut(s) 299, 520, 559, 1610
BpuMI CCSGG 2 cut(s) 992, 1488
BsaHI GRCGYC 2 cut(s) 344, 1398
BsaJI CCNNGG 9 cut(s) 27, 235, 355, 388, 956, 1015, 1350, 1779, 1795
BsaXI ACNNNNNCTCC 2 cut(s) 515, 545
Bsc4I CCNNNNNNNGG 2 cut(s) 394, 695
Bse1I ACTGG 5 cut(s) 1296, 1412, 1448, 1519, 1735
Bse21I CCTNAGG 1 cut(s) 1188
Bse3DI GCAATG 3 cut(s) 822, 1108, 2238
BseBI CCWGG 7 cut(s) 291, 390, 682, 696, 1352, 1797, 2201
BseDI CCNNGG 9 cut(s) 27, 235, 355, 388, 956, 1015, 1350, 1779, 1795
BseGI GGATG 8 cut(s) 601, 1061, 1239, 1252, 1497, 1569, 1583, 1682
BseLI CCNNNNNNNGG 2 cut(s) 394, 695
BseMI GCAATG 3 cut(s) 822, 1108, 2238
BseMII CTCAG 5 cut(s) 339, 438, 1179, 1590, 2102
BseNI ACTGG 5 cut(s) 1296, 1412, 1448, 1519, 1735
BseRI GAGGAG 2 cut(s) 523, 527
BseSI GKGCMC 1 cut(s) 1598
BseYI CCCAGC 3 cut(s) 1151, 1505, 1763
BsgI GTGCAG 2 cut(s) 927, 1539
Bsh1236I CGCG 1 cut(s) 248
BshFI GGCC 7 cut(s) 445, 480, 812, 925, 1311, 1616, 1800
BshNI GGYRCC 1 cut(s) 1991
BsiHKCI CYCGRG 2 cut(s) 753, 940
BsiSI CCGG 3 cut(s) 912, 991, 1487
BslFI GGGAC 3 cut(s) 670, 739, 2220
BslI CCNNNNNNNGG 2 cut(s) 394, 695
BsmAI GTCTC 4 cut(s) 479, 1456, 1884, 2053
BsmFI GGGAC 3 cut(s) 670, 739, 2220
BsmI GAATGC 2 cut(s) 1119, 1424
BsnI GGCC 7 cut(s) 445, 480, 812, 925, 1311, 1616, 1800
BsoBI CYCGRG 2 cut(s) 753, 940
Bsp1286I GDGCHC 2 cut(s) 1598, 1652
Bsp143I GATC 7 cut(s) 392, 629, 1171, 1357, 1810, 1930, 2088
BspACI CCGC 9 cut(s) 63, 246, 813, 1056, 1205, 1244, 1555, 1608, 1617
BspANI GGCC 7 cut(s) 445, 480, 812, 925, 1311, 1616, 1800
BspCNI CTCAG 5 cut(s) 340, 439, 1180, 1589, 2103
BspFNI CGCG 1 cut(s) 248
BspHI TCATGA 1 cut(s) 1674
BspLI GGNNCC 4 cut(s) 686, 924, 1649, 1993
BspMAI CTGCAG 2 cut(s) 679, 1657
BspMI ACCTGC 2 cut(s) 1907, 1979
BspOI GCTAGC 1 cut(s) 705
BspPI GGATC 2 cut(s) 400, 1352
BspT107I GGYRCC 1 cut(s) 1991
BsrBI CCGCTC 1 cut(s) 1608
BsrDI GCAATG 3 cut(s) 822, 1108, 2238
BsrI ACTGG 5 cut(s) 1296, 1412, 1448, 1519, 1735
BssECI CCNNGG 9 cut(s) 27, 235, 355, 388, 956, 1015, 1350, 1779, 1795
BssMI GATC 7 cut(s) 392, 629, 1171, 1357, 1810, 1930, 2088
BssNI GRCGYC 2 cut(s) 344, 1398
BssSI CACGAG 1 cut(s) 2005
BssT1I CCWWGG 3 cut(s) 355, 956, 1015
Bst2BI CACGAG 1 cut(s) 2005
Bst2UI CCWGG 7 cut(s) 291, 390, 682, 696, 1352, 1797, 2201
Bst4CI ACNGT 8 cut(s) 298, 854, 970, 1130, 1392, 1760, 1982, 2149
Bst6I CTCTTC 1 cut(s) 713
BstACI GRCGYC 2 cut(s) 344, 1398
BstAPI GCANNNNNTGC 2 cut(s) 1116, 1148
BstC8I GCNNGC 5 cut(s) 138, 703, 750, 779, 2197
BstDEI CTNAG 7 cut(s) 348, 447, 782, 1188, 1576, 1832, 2111
BstDSI CCRYGG 3 cut(s) 27, 235, 1779
BstENI CCTNNNNNAGG 1 cut(s) 693
BstF5I GGATG 8 cut(s) 601, 1061, 1239, 1252, 1497, 1569, 1583, 1682
BstFNI CGCG 1 cut(s) 248
BstHHI GCGC 2 cut(s) 40, 1369
BstKTI GATC 7 cut(s) 395, 632, 1174, 1360, 1813, 1933, 2091
BstMAI GTCTC 4 cut(s) 479, 1456, 1884, 2053
BstMBI GATC 7 cut(s) 392, 629, 1171, 1357, 1810, 1930, 2088
BstNI CCWGG 7 cut(s) 291, 390, 682, 696, 1352, 1797, 2201
BstNSI RCATGY 4 cut(s) 211, 377, 1604, 1772
BstPAI GACNNNNGTC 1 cut(s) 471
BstSCI CCNGG 9 cut(s) 289, 388, 680, 694, 990, 1350, 1486, 1795, 2199
BstSFI CTRYAG 3 cut(s) 675, 1653, 1953
BstSLI GKGCMC 1 cut(s) 1598
BstUI CGCG 1 cut(s) 248
BstV2I GAAGAC 5 cut(s) 81, 107, 376, 1391, 2144
BstX2I RGATCY 3 cut(s) 1357, 1930, 2088
BstXI CCANNNNNNTGG 1 cut(s) 1319
BstYI RGATCY 3 cut(s) 1357, 1930, 2088
Bsu36I CCTNAGG 1 cut(s) 1188
BsuRI GGCC 7 cut(s) 445, 480, 812, 925, 1311, 1616, 1800
BtgI CCRYGG 3 cut(s) 27, 235, 1779
BtrI CACGTC 1 cut(s) 1282
BtsCI GGATG 8 cut(s) 601, 1061, 1239, 1252, 1497, 1569, 1583, 1682
BtsI GCAGTG 1 cut(s) 672
BtsIMutI CAGTG 5 cut(s) 672, 966, 1419, 2088, 2108
BveI ACCTGC 2 cut(s) 1907, 1979
Cac8I GCNNGC 5 cut(s) 138, 703, 750, 779, 2197
CaiI CAGNNNCTG 2 cut(s) 269, 296
CciI TCATGA 1 cut(s) 1674
CfoI GCGC 2 cut(s) 40, 1369
Cfr13I GGNCC 6 cut(s) 479, 684, 923, 1439, 1526, 2027
CseI GACGC 3 cut(s) 352, 1387, 2067
Csp6I GTAC 5 cut(s) 106, 1463, 1581, 1992, 2181
CviQI GTAC 5 cut(s) 106, 1463, 1581, 1992, 2181
DdeI CTNAG 7 cut(s) 348, 447, 782, 1188, 1576, 1832, 2111
DpnI GATC 7 cut(s) 394, 631, 1173, 1359, 1812, 1932, 2090
DpnII GATC 7 cut(s) 392, 629, 1171, 1357, 1810, 1930, 2088
DraIII CACNNNGTG 1 cut(s) 2227
EaeI YGGCCR 3 cut(s) 810, 1309, 1614
Eam1104I CTCTTC 1 cut(s) 713
EarI CTCTTC 1 cut(s) 713
EciI GGCGGA 2 cut(s) 1071, 1259
Eco130I CCWWGG 3 cut(s) 355, 956, 1015
Eco24I GRGCYC 1 cut(s) 1652
Eco47I GGWCC 4 cut(s) 684, 1439, 1526, 2027
Eco57I CTGAAG 1 cut(s) 705
Eco81I CCTNAGG 1 cut(s) 1188
Eco88I CYCGRG 2 cut(s) 753, 940
EcoNI CCTNNNNNAGG 1 cut(s) 693
EcoRII CCWGG 7 cut(s) 289, 388, 680, 694, 1350, 1795, 2199
EcoT14I CCWWGG 3 cut(s) 355, 956, 1015
EcoT22I ATGCAT 1 cut(s) 1121
EcoT38I GRGCYC 1 cut(s) 1652
ErhI CCWWGG 3 cut(s) 355, 956, 1015
FalI AAGNNNNNCTT 4 cut(s) 579, 611, 878, 910
FaqI GGGAC 3 cut(s) 670, 739, 2220
FauI CCCGC 2 cut(s) 1548, 1601
FauNDI CATATG 2 cut(s) 1006, 2128
FbaI TGATCA 1 cut(s) 1171
FblI GTMKAC 3 cut(s) 1050, 1773, 1956
FokI GGATG 8 cut(s) 588, 1048, 1246, 1259, 1484, 1556, 1570, 1669
FriOI GRGCYC 1 cut(s) 1652
FspBI CTAG 6 cut(s) 702, 1071, 1434, 1502, 1538, 2163
GlaI GCGC 2 cut(s) 39, 1368
GsaI CCCAGC 3 cut(s) 1155, 1509, 1767
HaeIII GGCC 7 cut(s) 445, 480, 812, 925, 1311, 1616, 1800
HapII CCGG 3 cut(s) 912, 991, 1487
HgaI GACGC 3 cut(s) 352, 1387, 2067
HhaI GCGC 2 cut(s) 40, 1369
Hin1I GRCGYC 2 cut(s) 344, 1398
Hin6I GCGC 2 cut(s) 38, 1367
HinP1I GCGC 2 cut(s) 38, 1367
HindIII AAGCTT 1 cut(s) 1834
HinfI GANTC 9 cut(s) 197, 256, 431, 623, 891, 1882, 1904, 2011, 2063
HpaII CCGG 3 cut(s) 912, 991, 1487
HphI GGTGA 5 cut(s) 624, 1401, 1570, 1628, 1888
Hpy166II GTNNAC 7 cut(s) 1051, 1495, 1512, 1774, 1807, 1957, 2030
Hpy188I TCNGA 7 cut(s) 164, 196, 342, 802, 1458, 2016, 2212
Hpy188III TCNNGA 7 cut(s) 366, 627, 1124, 1381, 1434, 1675, 1873
Hpy8I GTNNAC 7 cut(s) 1051, 1495, 1512, 1774, 1807, 1957, 2030
Hpy99I CGWCG 2 cut(s) 1283, 1403
HpyAV CCTTC 1 cut(s) 880
HpyCH4III ACNGT 8 cut(s) 298, 854, 970, 1130, 1392, 1760, 1982, 2149
HpyCH4IV ACGT 3 cut(s) 104, 228, 1281
HpyF3I CTNAG 7 cut(s) 348, 447, 782, 1188, 1576, 1832, 2111
HpySE526I ACGT 3 cut(s) 104, 228, 1281
Hsp92I GRCGYC 2 cut(s) 344, 1398
HspAI GCGC 2 cut(s) 38, 1367
KpnI GGTACC 1 cut(s) 1995
Ksp22I TGATCA 1 cut(s) 1171
Kzo9I GATC 7 cut(s) 392, 629, 1171, 1357, 1810, 1930, 2088
LmnI GCTCC 6 cut(s) 698, 803, 866, 958, 1647, 1985
LweI GCATC 2 cut(s) 1237, 1294
MaeI CTAG 6 cut(s) 702, 1071, 1434, 1502, 1538, 2163
MaeII ACGT 3 cut(s) 104, 228, 1281
MaeIII GTNAC 2 cut(s) 2149, 2158
MalI GATC 7 cut(s) 394, 631, 1173, 1359, 1812, 1932, 2090
MbiI CCGCTC 1 cut(s) 1608
MboI GATC 7 cut(s) 392, 629, 1171, 1357, 1810, 1930, 2088
MfeI CAATTG 1 cut(s) 66
MflI RGATCY 3 cut(s) 1357, 1930, 2088
MhlI GDGCHC 2 cut(s) 1598, 1652
MlsI TGGCCA 1 cut(s) 1311
MluCI AATT 9 cut(s) 66, 314, 1158, 1254, 1272, 1877, 1915, 1936, 1969
MluNI TGGCCA 1 cut(s) 1311
MlyI GAGTC 4 cut(s) 250, 1876, 2005, 2057
MmeI TCCRAC 3 cut(s) 174, 1504, 1906
Mox20I TGGCCA 1 cut(s) 1311
Mph1103I ATGCAT 1 cut(s) 1121
MroXI GAANNNNTTC 1 cut(s) 298
MscI TGGCCA 1 cut(s) 1311
MseI TTAA 6 cut(s) 399, 930, 1257, 1566, 1662, 1935
MslI CAYNNNNRTG 2 cut(s) 116, 1556
Msp20I TGGCCA 1 cut(s) 1311
MspA1I CMGCKG 2 cut(s) 380, 2250
MspI CCGG 3 cut(s) 912, 991, 1487
MspR9I CCNGG 9 cut(s) 291, 390, 682, 696, 992, 1352, 1488, 1797, 2201
MunI CAATTG 1 cut(s) 66
Mva1269I GAATGC 2 cut(s) 1119, 1424
MvaI CCWGG 7 cut(s) 291, 390, 682, 696, 1352, 1797, 2201
MvnI CGCG 1 cut(s) 248
NciI CCSGG 2 cut(s) 992, 1488
NdeI CATATG 2 cut(s) 1006, 2128
NdeII GATC 7 cut(s) 392, 629, 1171, 1357, 1810, 1930, 2088
NheI GCTAGC 1 cut(s) 701
NlaIV GGNNCC 4 cut(s) 686, 924, 1649, 1993
NmeAIII GCCGAG 2 cut(s) 456, 513
NmuCI GTSAC 1 cut(s) 2149
NsiI ATGCAT 1 cut(s) 1121
NspI RCATGY 4 cut(s) 211, 377, 1604, 1772
PaeR7I CTCGAG 2 cut(s) 753, 940
PagI TCATGA 1 cut(s) 1674
PaqCI CACCTGC 1 cut(s) 1907
PciI ACATGT 1 cut(s) 1600
PctI GAATGC 2 cut(s) 1119, 1424
PdmI GAANNNNTTC 1 cut(s) 298
PfeI GAWTC 5 cut(s) 197, 431, 623, 891, 1904
PfoI TCCNGGA 1 cut(s) 694
PleI GAGTC 4 cut(s) 250, 1876, 2005, 2057
PpsI GAGTC 4 cut(s) 250, 1876, 2005, 2057
PscI ACATGT 1 cut(s) 1600
PshAI GACNNNNGTC 1 cut(s) 471
Psp6I CCWGG 7 cut(s) 289, 388, 680, 694, 1350, 1795, 2199
PspFI CCCAGC 3 cut(s) 1151, 1505, 1763
PspGI CCWGG 7 cut(s) 289, 388, 680, 694, 1350, 1795, 2199
PspN4I GGNNCC 4 cut(s) 686, 924, 1649, 1993
PspPI GGNCC 6 cut(s) 479, 684, 923, 1439, 1526, 2027
PspXI VCTCGAGB 2 cut(s) 753, 940
PstI CTGCAG 2 cut(s) 679, 1657
PstNI CAGNNNCTG 2 cut(s) 269, 296
PsuI RGATCY 3 cut(s) 1357, 1930, 2088
PvuII CAGCTG 2 cut(s) 380, 2250
RsaI GTAC 5 cut(s) 107, 1464, 1582, 1993, 2182
RsaNI GTAC 5 cut(s) 106, 1463, 1581, 1992, 2181
RseI CAYNNNNRTG 2 cut(s) 116, 1556
SaqAI TTAA 6 cut(s) 399, 930, 1257, 1566, 1662, 1935
Sau3AI GATC 7 cut(s) 392, 629, 1171, 1357, 1810, 1930, 2088
Sau96I GGNCC 6 cut(s) 479, 684, 923, 1439, 1526, 2027
SchI GAGTC 4 cut(s) 250, 1876, 2005, 2057
ScrFI CCNGG 9 cut(s) 291, 390, 682, 696, 992, 1352, 1488, 1797, 2201
SduI GDGCHC 2 cut(s) 1598, 1652
SfaNI GCATC 2 cut(s) 1237, 1294
SfcI CTRYAG 3 cut(s) 675, 1653, 1953
Sfr274I CTCGAG 2 cut(s) 753, 940
SinI GGWCC 4 cut(s) 684, 1439, 1526, 2027
SlaI CTCGAG 2 cut(s) 753, 940
SmiMI CAYNNNNRTG 2 cut(s) 116, 1556
SmlI CTYRAG 6 cut(s) 278, 535, 574, 753, 940, 1625
SmoI CTYRAG 6 cut(s) 278, 535, 574, 753, 940, 1625
SpeI ACTAGT 1 cut(s) 2162
Sse9I AATT 9 cut(s) 66, 314, 1158, 1254, 1272, 1877, 1915, 1936, 1969
SsiI CCGC 9 cut(s) 63, 246, 813, 1056, 1205, 1244, 1555, 1608, 1617
SspMI CTAG 6 cut(s) 702, 1071, 1434, 1502, 1538, 2163
StyD4I CCNGG 9 cut(s) 289, 388, 680, 694, 990, 1350, 1486, 1795, 2199
StyI CCWWGG 3 cut(s) 355, 956, 1015
TaaI ACNGT 8 cut(s) 298, 854, 970, 1130, 1392, 1760, 1982, 2149
TaiI ACGT 3 cut(s) 107, 231, 1284
TaqI TCGA 5 cut(s) 668, 754, 941, 1024, 1401
TasI AATT 9 cut(s) 66, 314, 1158, 1254, 1272, 1877, 1915, 1936, 1969
TauI GCSGC 2 cut(s) 815, 1619
TfiI GAWTC 5 cut(s) 197, 431, 623, 891, 1904
Tru1I TTAA 6 cut(s) 399, 930, 1257, 1566, 1662, 1935
Tru9I TTAA 6 cut(s) 399, 930, 1257, 1566, 1662, 1935
TscAI CASTG 5 cut(s) 679, 973, 1419, 2088, 2115
TseFI GTSAC 1 cut(s) 2149
Tsp45I GTSAC 1 cut(s) 2149
TspDTI ATGAA 6 cut(s) 7, 110, 339, 600, 623, 627
TspRI CASTG 5 cut(s) 679, 973, 1419, 2088, 2115
VpaK11BI GGWCC 4 cut(s) 684, 1439, 1526, 2027
XagI CCTNNNNNAGG 1 cut(s) 693
XapI RAATTY 1 cut(s) 1969
XbaI TCTAGA 1 cut(s) 1433
XceI RCATGY 4 cut(s) 211, 377, 1604, 1772
XhoI CTCGAG 2 cut(s) 753, 940
XmiI GTMKAC 3 cut(s) 1050, 1773, 1956
XmnI GAANNNNTTC 1 cut(s) 298
XspI CTAG 6 cut(s) 702, 1071, 1434, 1502, 1538, 2163
Zsp2I ATGCAT 1 cut(s) 1121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.