subtilisin-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 3 cut(s) | 410, 431, 830 |
| AatII | GACGTC | 1 cut(s) | 408 |
| AccB1I | GGYRCC | 5 cut(s) | 497, 512, 554, 1439, 1571 |
| AccBSI | CCGCTC | 2 cut(s) | 1662, 2022 |
| AccI | GTMKAC | 3 cut(s) | 1154, 1599, 1860 |
| AclI | AACGTT | 1 cut(s) | 837 |
| AcsI | RAATTY | 1 cut(s) | 2011 |
| AcvI | CACGTG | 2 cut(s) | 1657, 1825 |
| AcyI | GRCGYC | 4 cut(s) | 254, 405, 1121, 1572 |
| AfaI | GTAC | 6 cut(s) | 167, 320, 563, 968, 2054, 2093 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 289, 672, 726, 1435, 1837, 1849 |
| AflIII | ACRYGT | 2 cut(s) | 171, 1822 |
| AgeI | ACCGGT | 1 cut(s) | 1586 |
| AgsI | TTSAA | 5 cut(s) | 316, 523, 1621, 1906, 2050 |
| AjiI | CACGTC | 2 cut(s) | 1638, 1777 |
| AjnI | CCWGG | 5 cut(s) | 557, 617, 712, 743, 1540 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 276, 308 |
| AleI | CACNNNNGTG | 1 cut(s) | 2072 |
| Alw21I | GWGCWC | 4 cut(s) | 104, 153, 327, 941 |
| Alw26I | GTCTC | 1 cut(s) | 624 |
| Ama87I | CYCGRG | 7 cut(s) | 287, 308, 344, 1242, 1488, 2056, 2240 |
| ApaI | GGGCCC | 2 cut(s) | 1690, 2081 |
| ApeKI | GCWGC | 4 cut(s) | 247, 1345, 1675, 2226 |
| ApoI | RAATTY | 1 cut(s) | 2011 |
| AsiGI | ACCGGT | 1 cut(s) | 1586 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1948 |
| AsuHPI | GGTGA | 5 cut(s) | 1918, 2029, 2059, 2137, 2203 |
| AvaI | CYCGRG | 7 cut(s) | 287, 308, 344, 1242, 1488, 2056, 2240 |
| AvaII | GGWCC | 8 cut(s) | 473, 621, 675, 956, 1246, 1291, 1566, 1828 |
| BaeGI | GKGCMC | 5 cut(s) | 500, 1016, 1442, 1690, 2081 |
| BalI | TGGCCA | 4 cut(s) | 5, 439, 734, 806 |
| BamHI | GGATCC | 2 cut(s) | 716, 1405 |
| BanI | GGYRCC | 5 cut(s) | 497, 512, 554, 1439, 1571 |
| BanII | GRGCYC | 4 cut(s) | 327, 1690, 1706, 2081 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 1141, 1173, 2168, 2200 |
| BauI | CACGAG | 1 cut(s) | 934 |
| BbrPI | CACGTG | 2 cut(s) | 1657, 1825 |
| BbsI | GAAGAC | 1 cut(s) | 2025 |
| Bbv12I | GWGCWC | 4 cut(s) | 104, 153, 327, 941 |
| BbvCI | CCTCAGC | 1 cut(s) | 1043 |
| BbvI | GCAGC | 4 cut(s) | 259, 1332, 1662, 2238 |
| BceAI | ACGGC | 8 cut(s) | 250, 655, 1330, 1371, 1390, 1434, 1559, 1761 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 119, 153, 1429, 1463, 1796, 1830 |
| BciT130I | CCWGG | 5 cut(s) | 559, 619, 714, 745, 1542 |
| BciVI | GTATCC | 1 cut(s) | 698 |
| BcoDI | GTCTC | 1 cut(s) | 624 |
| BfaI | CTAG | 2 cut(s) | 12, 87 |
| BfoI | RGCGCY | 2 cut(s) | 1575, 1727 |
| BfuI | GTATCC | 1 cut(s) | 698 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1745 |
| BmcAI | AGTACT | 1 cut(s) | 2054 |
| Bme18I | GGWCC | 8 cut(s) | 473, 621, 675, 956, 1246, 1291, 1566, 1828 |
| BmeT110I | CYCGRG | 7 cut(s) | 287, 308, 344, 1242, 1488, 2056, 2240 |
| BmgBI | CACGTC | 2 cut(s) | 1638, 1777 |
| BmsI | GCATC | 2 cut(s) | 195, 261 |
| BpiI | GAAGAC | 1 cut(s) | 2025 |
| BpmI | CTGGAG | 1 cut(s) | 450 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1043 |
| BpuEI | CTTGAG | 2 cut(s) | 260, 1664 |
| BsaAI | YACGTR | 2 cut(s) | 1657, 1825 |
| BsaBI | GATNNNNATC | 3 cut(s) | 929, 1410, 2282 |
| BsaHI | GRCGYC | 4 cut(s) | 254, 405, 1121, 1572 |
| BsaWI | WCCGGW | 5 cut(s) | 464, 1192, 1465, 1586, 1784 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 289, 672, 726, 1435, 1837, 1849 |
| Bse118I | RCCGGY | 2 cut(s) | 1348, 1586 |
| Bse1I | ACTGG | 4 cut(s) | 167, 399, 433, 1023 |
| Bse8I | GATNNNNATC | 3 cut(s) | 929, 1410, 2282 |
| BseBI | CCWGG | 5 cut(s) | 559, 619, 714, 745, 1542 |
| BseGI | GGATG | 5 cut(s) | 27, 228, 735, 1793, 2119 |
| BseJI | GATNNNNATC | 3 cut(s) | 929, 1410, 2282 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 289, 672, 726, 1435, 1837, 1849 |
| BseMII | CTCAG | 2 cut(s) | 1057, 1083 |
| BseNI | ACTGG | 4 cut(s) | 167, 399, 433, 1023 |
| BseRI | GAGGAG | 2 cut(s) | 275, 1334 |
| BseSI | GKGCMC | 5 cut(s) | 500, 1016, 1442, 1690, 2081 |
| BseX3I | CGGCCG | 4 cut(s) | 198, 942, 1419, 2082 |
| BseXI | GCAGC | 4 cut(s) | 259, 1332, 1662, 2238 |
| BseYI | CCCAGC | 1 cut(s) | 959 |
| Bsh1285I | CGRYCG | 8 cut(s) | 201, 897, 945, 1228, 1422, 1599, 1721, 2085 |
| BshNI | GGYRCC | 5 cut(s) | 497, 512, 554, 1439, 1571 |
| BshTI | ACCGGT | 1 cut(s) | 1586 |
| BsiEI | CGRYCG | 8 cut(s) | 201, 897, 945, 1228, 1422, 1599, 1721, 2085 |
| BsiHKAI | GWGCWC | 4 cut(s) | 104, 153, 327, 941 |
| BsiHKCI | CYCGRG | 7 cut(s) | 287, 308, 344, 1242, 1488, 2056, 2240 |
| BslFI | GGGAC | 3 cut(s) | 607, 896, 942 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 289, 672, 726, 1435, 1837, 1849 |
| BsmAI | GTCTC | 1 cut(s) | 624 |
| BsmBI | CGTCTC | 1 cut(s) | 624 |
| BsmFI | GGGAC | 3 cut(s) | 607, 896, 942 |
| BsoBI | CYCGRG | 7 cut(s) | 287, 308, 344, 1242, 1488, 2056, 2240 |
| Bsp120I | GGGCCC | 2 cut(s) | 1686, 2077 |
| Bsp19I | CCATGG | 1 cut(s) | 2109 |
| Bsp68I | TCGCGA | 1 cut(s) | 1026 |
| BspCNI | CTCAG | 2 cut(s) | 1056, 1082 |
| BspQI | GCTCTTC | 1 cut(s) | 383 |
| BspT107I | GGYRCC | 5 cut(s) | 497, 512, 554, 1439, 1571 |
| BsrBI | CCGCTC | 2 cut(s) | 1662, 2022 |
| BsrFI | RCCGGY | 2 cut(s) | 1348, 1586 |
| BsrI | ACTGG | 4 cut(s) | 167, 399, 433, 1023 |
| BssAI | RCCGGY | 2 cut(s) | 1348, 1586 |
| BssNI | GRCGYC | 4 cut(s) | 254, 405, 1121, 1572 |
| BssSI | CACGAG | 1 cut(s) | 934 |
| BssT1I | CCWWGG | 3 cut(s) | 609, 1443, 2109 |
| Bst2BI | CACGAG | 1 cut(s) | 934 |
| Bst2UI | CCWGG | 5 cut(s) | 559, 619, 714, 745, 1542 |
| Bst4CI | ACNGT | 8 cut(s) | 971, 1003, 1058, 1261, 1814, 1873, 2044, 2191 |
| Bst6I | CTCTTC | 2 cut(s) | 383, 2174 |
| BstACI | GRCGYC | 4 cut(s) | 254, 405, 1121, 1572 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1768 |
| BstBAI | YACGTR | 2 cut(s) | 1657, 1825 |
| BstC8I | GCNNGC | 6 cut(s) | 138, 487, 694, 1012, 1350, 1652 |
| BstDEI | CTNAG | 3 cut(s) | 1043, 1069, 1555 |
| BstDSI | CCRYGG | 9 cut(s) | 63, 232, 643, 666, 989, 1087, 1260, 2109, 2163 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1433 |
| BstF5I | GGATG | 5 cut(s) | 27, 228, 735, 1793, 2119 |
| BstH2I | RGCGCY | 2 cut(s) | 1575, 1727 |
| BstMAI | GTCTC | 1 cut(s) | 624 |
| BstMCI | CGRYCG | 8 cut(s) | 201, 897, 945, 1228, 1422, 1599, 1721, 2085 |
| BstNI | CCWGG | 5 cut(s) | 559, 619, 714, 745, 1542 |
| BstSLI | GKGCMC | 5 cut(s) | 500, 1016, 1442, 1690, 2081 |
| BstV1I | GCAGC | 4 cut(s) | 259, 1332, 1662, 2238 |
| BstV2I | GAAGAC | 1 cut(s) | 2025 |
| BstX2I | RGATCY | 5 cut(s) | 716, 899, 924, 1405, 1984 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1111, 1591 |
| BstYI | RGATCY | 5 cut(s) | 716, 899, 924, 1405, 1984 |
| BstZI | CGGCCG | 4 cut(s) | 198, 942, 1419, 2082 |
| BsuI | GTATCC | 1 cut(s) | 698 |
| BtgI | CCRYGG | 9 cut(s) | 63, 232, 643, 666, 989, 1087, 1260, 2109, 2163 |
| BtgZI | GCGATG | 2 cut(s) | 926, 1392 |
| BtrI | CACGTC | 2 cut(s) | 1638, 1777 |
| BtsCI | GGATG | 5 cut(s) | 27, 228, 735, 1793, 2119 |
| BtsIMutI | CAGTG | 5 cut(s) | 160, 406, 426, 1016, 2040 |
| BtuMI | TCGCGA | 1 cut(s) | 1026 |
| Cac8I | GCNNGC | 6 cut(s) | 138, 487, 694, 1012, 1350, 1652 |
| Cfr10I | RCCGGY | 2 cut(s) | 1348, 1586 |
| Cfr42I | CCGCGG | 4 cut(s) | 66, 669, 1090, 2166 |
| Cfr9I | CCCGGG | 2 cut(s) | 287, 1242 |
| CseI | GACGC | 6 cut(s) | 92, 162, 179, 262, 1129, 1343 |
| Csp6I | GTAC | 6 cut(s) | 166, 319, 562, 967, 2053, 2092 |
| CspAI | ACCGGT | 1 cut(s) | 1586 |
| CviAII | CATG | 6 cut(s) | 7, 118, 847, 1174, 1288, 2110 |
| CviQI | GTAC | 6 cut(s) | 166, 319, 562, 967, 2053, 2092 |
| DdeI | CTNAG | 3 cut(s) | 1043, 1069, 1555 |
| DinI | GGCGCC | 1 cut(s) | 1573 |
| DrdI | GACNNNNNNGTC | 3 cut(s) | 410, 431, 830 |
| DseDI | GACNNNNNNGTC | 3 cut(s) | 410, 431, 830 |
| EagI | CGGCCG | 4 cut(s) | 198, 942, 1419, 2082 |
| Eam1104I | CTCTTC | 2 cut(s) | 383, 2174 |
| EarI | CTCTTC | 2 cut(s) | 383, 2174 |
| EciI | GGCGGA | 4 cut(s) | 83, 95, 281, 876 |
| Ecl136II | GAGCTC | 1 cut(s) | 325 |
| EclXI | CGGCCG | 4 cut(s) | 198, 942, 1419, 2082 |
| Eco130I | CCWWGG | 3 cut(s) | 609, 1443, 2109 |
| Eco24I | GRGCYC | 4 cut(s) | 327, 1690, 1706, 2081 |
| Eco47I | GGWCC | 8 cut(s) | 473, 621, 675, 956, 1246, 1291, 1566, 1828 |
| Eco52I | CGGCCG | 4 cut(s) | 198, 942, 1419, 2082 |
| Eco53kI | GAGCTC | 1 cut(s) | 325 |
| Eco72I | CACGTG | 2 cut(s) | 1657, 1825 |
| Eco88I | CYCGRG | 7 cut(s) | 287, 308, 344, 1242, 1488, 2056, 2240 |
| EcoICRI | GAGCTC | 1 cut(s) | 325 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1433 |
| EcoO109I | RGGNCCY | 4 cut(s) | 593, 621, 956, 1686 |
| EcoRII | CCWGG | 5 cut(s) | 557, 617, 712, 743, 1540 |
| EcoT14I | CCWWGG | 3 cut(s) | 609, 1443, 2109 |
| EcoT38I | GRGCYC | 4 cut(s) | 327, 1690, 1706, 2081 |
| EgeI | GGCGCC | 1 cut(s) | 1573 |
| EheI | GGCGCC | 1 cut(s) | 1573 |
| ErhI | CCWWGG | 3 cut(s) | 609, 1443, 2109 |
| Esp3I | CGTCTC | 1 cut(s) | 624 |
| FaeI | CATG | 6 cut(s) | 10, 121, 850, 1177, 1291, 2113 |
| FaiI | YATR | 9 cut(s) | 8, 119, 809, 848, 1175, 1289, 1341, 1343, 2111 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 911, 943 |
| FaqI | GGGAC | 3 cut(s) | 607, 896, 942 |
| FatI | CATG | 6 cut(s) | 6, 117, 846, 1173, 1287, 2109 |
| FauI | CCCGC | 6 cut(s) | 664, 770, 946, 1003, 1659, 1714 |
| FauNDI | CATATG | 1 cut(s) | 1341 |
| FblI | GTMKAC | 3 cut(s) | 1154, 1599, 1860 |
| FokI | GGATG | 5 cut(s) | 34, 215, 742, 1780, 2126 |
| FriOI | GRGCYC | 4 cut(s) | 327, 1690, 1706, 2081 |
| FspBI | CTAG | 2 cut(s) | 12, 87 |
| GsaI | CCCAGC | 1 cut(s) | 963 |
| GsuI | CTGGAG | 1 cut(s) | 450 |
| HaeII | RGCGCY | 2 cut(s) | 1575, 1727 |
| HgaI | GACGC | 6 cut(s) | 92, 162, 179, 262, 1129, 1343 |
| Hin1I | GRCGYC | 4 cut(s) | 254, 405, 1121, 1572 |
| Hin1II | CATG | 6 cut(s) | 10, 121, 850, 1177, 1291, 2113 |
| HincII | GTYRAC | 4 cut(s) | 835, 1237, 1549, 1600 |
| HindII | GTYRAC | 4 cut(s) | 835, 1237, 1549, 1600 |
| HphI | GGTGA | 5 cut(s) | 1918, 2029, 2059, 2137, 2203 |
| Hpy166II | GTNNAC | 9 cut(s) | 115, 835, 1155, 1237, 1549, 1600, 1828, 1861, 2040 |
| Hpy188III | TCNNGA | 9 cut(s) | 19, 308, 344, 799, 928, 1025, 1249, 1488, 2214 |
| Hpy8I | GTNNAC | 9 cut(s) | 115, 835, 1155, 1237, 1549, 1600, 1828, 1861, 2040 |
| HpyAV | CCTTC | 6 cut(s) | 487, 913, 1198, 1470, 1813, 2007 |
| HpyCH4III | ACNGT | 8 cut(s) | 971, 1003, 1058, 1261, 1814, 1873, 2044, 2191 |
| HpyCH4IV | ACGT | 9 cut(s) | 405, 831, 837, 1637, 1656, 1776, 1824, 2090, 2195 |
| HpyCH4V | TGCA | 2 cut(s) | 1173, 1771 |
| HpyF3I | CTNAG | 3 cut(s) | 1043, 1069, 1555 |
| HpySE526I | ACGT | 9 cut(s) | 405, 831, 837, 1637, 1656, 1776, 1824, 2090, 2195 |
| Hsp92I | GRCGYC | 4 cut(s) | 254, 405, 1121, 1572 |
| Hsp92II | CATG | 6 cut(s) | 10, 121, 850, 1177, 1291, 2113 |
| KasI | GGCGCC | 1 cut(s) | 1571 |
| KflI | GGGWCCC | 1 cut(s) | 956 |
| KroI | GCCGGC | 1 cut(s) | 1348 |
| KroNI | GCCGGC | 1 cut(s) | 1350 |
| KspI | CCGCGG | 4 cut(s) | 66, 669, 1090, 2166 |
| LguI | GCTCTTC | 1 cut(s) | 383 |
| LmnI | GCTCC | 9 cut(s) | 99, 330, 689, 794, 991, 1321, 1389, 1701, 2140 |
| Lsp1109I | GCAGC | 4 cut(s) | 259, 1332, 1662, 2238 |
| LweI | GCATC | 2 cut(s) | 195, 261 |
| MaeI | CTAG | 2 cut(s) | 12, 87 |
| MaeII | ACGT | 9 cut(s) | 405, 831, 837, 1637, 1656, 1776, 1824, 2090, 2195 |
| MaeIII | GTNAC | 6 cut(s) | 401, 1052, 1970, 2183, 2191, 2203 |
| MbiI | CCGCTC | 2 cut(s) | 1662, 2022 |
| MboII | GAAGA | 6 cut(s) | 296, 370, 2025, 2140, 2188, 2191 |
| MflI | RGATCY | 5 cut(s) | 716, 899, 924, 1405, 1984 |
| MlsI | TGGCCA | 4 cut(s) | 5, 439, 734, 806 |
| MluCI | AATT | 3 cut(s) | 523, 1093, 2011 |
| MluI | ACGCGT | 1 cut(s) | 171 |
| MluNI | TGGCCA | 4 cut(s) | 5, 439, 734, 806 |
| Mly113I | GGCGCC | 1 cut(s) | 1572 |
| Mox20I | TGGCCA | 4 cut(s) | 5, 439, 734, 806 |
| MroNI | GCCGGC | 1 cut(s) | 1348 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1948 |
| MscI | TGGCCA | 4 cut(s) | 5, 439, 734, 806 |
| MslI | CAYNNNNRTG | 3 cut(s) | 800, 1640, 2072 |
| Msp20I | TGGCCA | 4 cut(s) | 5, 439, 734, 806 |
| MspA1I | CMGCKG | 5 cut(s) | 65, 250, 668, 1089, 2165 |
| MvaI | CCWGG | 5 cut(s) | 559, 619, 714, 745, 1542 |
| NaeI | GCCGGC | 1 cut(s) | 1350 |
| NarI | GGCGCC | 1 cut(s) | 1572 |
| NcoI | CCATGG | 1 cut(s) | 2109 |
| NdeI | CATATG | 1 cut(s) | 1341 |
| NgoMIV | GCCGGC | 1 cut(s) | 1348 |
| NlaIII | CATG | 6 cut(s) | 10, 121, 850, 1177, 1291, 2113 |
| NmeAIII | GCCGAG | 7 cut(s) | 176, 226, 509, 834, 887, 920, 962 |
| NmuCI | GTSAC | 2 cut(s) | 401, 2191 |
| NruI | TCGCGA | 1 cut(s) | 1026 |
| OliI | CACNNNNGTG | 1 cut(s) | 2072 |
| PaeR7I | CTCGAG | 2 cut(s) | 2056, 2240 |
| PciSI | GCTCTTC | 1 cut(s) | 383 |
| PcsI | WCGNNNNNNNCGW | 6 cut(s) | 306, 828, 1380, 1830, 2033, 2096 |
| PdiI | GCCGGC | 1 cut(s) | 1350 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1948 |
| PfeI | GAWTC | 4 cut(s) | 1165, 1281, 1411, 1452 |
| PflFI | GACNNNGTC | 2 cut(s) | 830, 1532 |
| PfoI | TCCNGGA | 1 cut(s) | 1160 |
| PinAI | ACCGGT | 1 cut(s) | 1586 |
| Ple19I | CGATCG | 3 cut(s) | 897, 1228, 1721 |
| PluTI | GGCGCC | 1 cut(s) | 1575 |
| PmaCI | CACGTG | 2 cut(s) | 1657, 1825 |
| PmlI | CACGTG | 2 cut(s) | 1657, 1825 |
| Ppu21I | YACGTR | 2 cut(s) | 1657, 1825 |
| PpuMI | RGGWCCY | 2 cut(s) | 621, 956 |
| Psp124BI | GAGCTC | 1 cut(s) | 327 |
| Psp1406I | AACGTT | 1 cut(s) | 837 |
| Psp5II | RGGWCCY | 2 cut(s) | 621, 956 |
| Psp6I | CCWGG | 5 cut(s) | 557, 617, 712, 743, 1540 |
| PspCI | CACGTG | 2 cut(s) | 1657, 1825 |
| PspFI | CCCAGC | 1 cut(s) | 959 |
| PspGI | CCWGG | 5 cut(s) | 557, 617, 712, 743, 1540 |
| PspOMI | GGGCCC | 2 cut(s) | 1686, 2077 |
| PspPPI | RGGWCCY | 2 cut(s) | 621, 956 |
| PspXI | VCTCGAGB | 2 cut(s) | 2056, 2240 |
| PsuI | RGATCY | 5 cut(s) | 716, 899, 924, 1405, 1984 |
| PsyI | GACNNNGTC | 2 cut(s) | 830, 1532 |
| PvuI | CGATCG | 3 cut(s) | 897, 1228, 1721 |
| PvuII | CAGCTG | 1 cut(s) | 250 |
| RruI | TCGCGA | 1 cut(s) | 1026 |
| RsaI | GTAC | 6 cut(s) | 167, 320, 563, 968, 2054, 2093 |
| RsaNI | GTAC | 6 cut(s) | 166, 319, 562, 967, 2053, 2092 |
| RseI | CAYNNNNRTG | 3 cut(s) | 800, 1640, 2072 |
| SacI | GAGCTC | 1 cut(s) | 327 |
| SacII | CCGCGG | 4 cut(s) | 66, 669, 1090, 2166 |
| SalI | GTCGAC | 1 cut(s) | 1598 |
| SapI | GCTCTTC | 1 cut(s) | 383 |
| ScaI | AGTACT | 1 cut(s) | 2054 |
| SfaNI | GCATC | 2 cut(s) | 195, 261 |
| SfoI | GGCGCC | 1 cut(s) | 1573 |
| Sfr274I | CTCGAG | 2 cut(s) | 2056, 2240 |
| Sfr303I | CCGCGG | 4 cut(s) | 66, 669, 1090, 2166 |
| SgrBI | CCGCGG | 4 cut(s) | 66, 669, 1090, 2166 |
| SinI | GGWCC | 8 cut(s) | 473, 621, 675, 956, 1246, 1291, 1566, 1828 |
| SlaI | CTCGAG | 2 cut(s) | 2056, 2240 |
| SmaI | CCCGGG | 2 cut(s) | 289, 1244 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 800, 1640, 2072 |
| SmlI | CTYRAG | 4 cut(s) | 275, 1679, 2056, 2240 |
| SmoI | CTYRAG | 4 cut(s) | 275, 1679, 2056, 2240 |
| Sse9I | AATT | 3 cut(s) | 523, 1093, 2011 |
| SspDI | GGCGCC | 1 cut(s) | 1571 |
| SspMI | CTAG | 2 cut(s) | 12, 87 |
| SstI | GAGCTC | 1 cut(s) | 327 |
| StyI | CCWWGG | 3 cut(s) | 609, 1443, 2109 |
| TaaI | ACNGT | 8 cut(s) | 971, 1003, 1058, 1261, 1814, 1873, 2044, 2191 |
| TaiI | ACGT | 9 cut(s) | 408, 834, 840, 1640, 1659, 1779, 1827, 2093, 2198 |
| TasI | AATT | 3 cut(s) | 523, 1093, 2011 |
| TatI | WGTACW | 1 cut(s) | 2052 |
| TfiI | GAWTC | 4 cut(s) | 1165, 1281, 1411, 1452 |
| TscAI | CASTG | 5 cut(s) | 167, 406, 433, 1023, 2047 |
| TseFI | GTSAC | 2 cut(s) | 401, 2191 |
| TseI | GCWGC | 4 cut(s) | 247, 1345, 1675, 2226 |
| Tsp45I | GTSAC | 2 cut(s) | 401, 2191 |
| TspGWI | ACGGA | 4 cut(s) | 379, 660, 667, 978 |
| TspMI | CCCGGG | 2 cut(s) | 287, 1242 |
| TspRI | CASTG | 5 cut(s) | 167, 406, 433, 1023, 2047 |
| Tth111I | GACNNNGTC | 2 cut(s) | 830, 1532 |
| VpaK11BI | GGWCC | 8 cut(s) | 473, 621, 675, 956, 1246, 1291, 1566, 1828 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1433 |
| XapI | RAATTY | 1 cut(s) | 2011 |
| XhoI | CTCGAG | 2 cut(s) | 2056, 2240 |
| XmaI | CCCGGG | 2 cut(s) | 287, 1242 |
| XmiI | GTMKAC | 3 cut(s) | 1154, 1599, 1860 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1948 |
| XspI | CTAG | 2 cut(s) | 12, 87 |
| ZraI | GACGTC | 1 cut(s) | 406 |
| ZrmI | AGTACT | 1 cut(s) | 2054 |