Rh6BG493000

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001634
Physical Location & Seq
Reverse (-)
69539181 .. 69541490
2310 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG493000.1

Sequence Viewer

Length: 1401 bp
ATGAAGAAGGCATTGAAATCCGTGCTTCTGGTAACGCTTATGGTTCTGGTTATCACTCACAACTGTGAGGTGGTAGCAGAAGAGAGTGAGCATGTGAAAAAGACGTACATCATTCACATGGACAAGTCCCAAATGCCCGCGAGTTTCGACGACGACCATGTCAACTGGTACGGCTCGTCGTTGAAAGCGGTCTCCGATTCAGCTGACATGCTTTACACGTACAAGACTGTCATCCATGGCTACTCAACAAGGCTAACTGCCGAGGAAGCTGAGCAGCTCGAAAAGCAGCCAGGAATTCTCTCTGTCCTGCCGGAAAGGAGGGACTTCCCGGCTTATGTCAGCCTTGGAAATGCAAAGAAATTTAGAGGTATATCTCTATACAGGGGAACGGCCTTGTCTAGTGGATTGATTCCACTTGTTGATGCTCGCAATGCGAGCAGTTCGAGTGGTGATCTGTGCAGTCCCGACAGTCTGATTCCGGCAAAGGTTGCCGGGAAGATTGTGGTATGTGACCGAGGGGGAACTCCGAGGGTTCAAAAGAGTCTGGTGGTGAAAAAGGCTGGTGGTGTGGGAATGATTTTGACCAACACCGAAACATATGGTGAAGAGCTTGTTGCTGATGCATATTTCTTGCCTACAGCAGCAGTTGGCCAGAAAGCTGGTGACGCAATAAAGAGCTATATTGCATCCGATTCTAATCCAACAGCAACAATTTCTCTTGGAGAAACGGAGTTAGGCGTCCAACCTTCACCGGTGGTGGCTGCGTTTAGCTCTAGAGGACCAAATTTTGTCACTCCAGAAGTACTCAAACCAGACCTGATAGCACCAGGAATGAACATCCTAGCTGGGTGGACGGGCGCAGTTGGACCAACTGGACTTCCTGAAGACAAGAGGAGGGTGGGCTTCAACATCATTTCAGTGGCAGCTCTTGACCCTGGCCTTGTCTACGATCTTGCTGTTGAGGACTACCTAAGTTTCCTCTGTGCCTTGAATTATTCCGCTTCTCAGATCAAGCAAACCACTAAGAAGGACTTCACCTGTGATTCAAGCAAGAAATACAGCCTCGGAGATTTGAATTACCCGTCCTTCTCTCTTGCTCTAGATACATCTTCAGGCCAAGGGGGTGGTACTGGTGCATCAACCACTTTAAAGTACACAAGGACTCTGACCAATGTGGGTGCCCCGGCAACATACAAGGTCTCAATGTCTTCACTGACCAGATCAGTGAAGATGTCCGTAGAACCAGAATCACTGAGTTTCAGTGATGCGTATGAGAAGAAGACATACACTGTGACTTTTGTTGCTAGCTCTTTGCCATCCGGTACAGCCAGCTTTGGTCGCTTGGAATGGTCTGATGGGAAACACATAGTAGGTAGTCCTATTGCTTACACCTGGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

466

Amino Acids

49.71

Weight (kDa)

6.62

Isoelectric Point (pI)

36.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 35 - 106 1.7e-17 Peptidase inhibitor I9
PA PF02225 138 - 224 3.5e-14 PA domain
fn3_6 PF17766 357 - 462 8e-25 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 158
AccB1I GGYRCC 1 cut(s) 1178
AccI GTMKAC 1 cut(s) 945
AccII CGCG 1 cut(s) 140
AciI CCGC 3 cut(s) 138, 188, 999
AcoI YGGCCR 1 cut(s) 649
AcsI RAATTY 3 cut(s) 294, 359, 784
AcuI CTGAAG 2 cut(s) 903, 1095
AcyI GRCGYC 1 cut(s) 738
AfaI GTAC 7 cut(s) 107, 170, 221, 804, 1129, 1154, 1324
AflIII ACRYGT 1 cut(s) 216
AgeI ACCGGT 1 cut(s) 751
AgsI TTSAA 7 cut(s) 16, 184, 536, 907, 991, 1047, 1075
AjnI CCWGG 4 cut(s) 289, 826, 934, 1391
AleI CACNNNNGTG 1 cut(s) 63
Alw26I GTCTC 2 cut(s) 196, 1204
AoxI GGCC 4 cut(s) 390, 649, 937, 1114
ApeKI GCWGC 5 cut(s) 274, 286, 641, 761, 923
ApoI RAATTY 3 cut(s) 294, 359, 784
AsiGI ACCGGT 1 cut(s) 751
Asp700I GAANNNNTTC 1 cut(s) 1031
AspLEI GCGC 1 cut(s) 860
AspS9I GGNCC 2 cut(s) 779, 866
AsuC2I CCSGG 3 cut(s) 329, 493, 1184
AsuHPI GGTGA 6 cut(s) 461, 562, 614, 674, 741, 1027
AsuNHI GCTAGC 1 cut(s) 1304
AvaII GGWCC 2 cut(s) 779, 866
BaeGI GKGCMC 1 cut(s) 1183
BalI TGGCCA 1 cut(s) 651
BanI GGYRCC 1 cut(s) 1178
BarI GAAGNNNNNNTAC 2 cut(s) 1268, 1300
BbsI GAAGAC 3 cut(s) 891, 1200, 1286
BbvI GCAGC 5 cut(s) 286, 298, 653, 748, 935
BccI CCATC 2 cut(s) 1324, 1349
BceAI ACGGC 2 cut(s) 187, 405
BciT130I CCWGG 4 cut(s) 291, 828, 936, 1393
BcnI CCSGG 3 cut(s) 329, 493, 1184
BcoDI GTCTC 2 cut(s) 196, 1204
BfaI CTAG 5 cut(s) 399, 774, 842, 1100, 1305
BfmI CTRYAG 1 cut(s) 636
BisI GCNGC 5 cut(s) 275, 287, 642, 762, 924
BlpI GCTNAGC 1 cut(s) 270
BlsI GCNGC 5 cut(s) 276, 288, 643, 763, 925
BmcAI AGTACT 1 cut(s) 804
Bme1390I CCNGG 7 cut(s) 291, 329, 493, 828, 936, 1184, 1393
Bme18I GGWCC 2 cut(s) 779, 866
BmgT120I GGNCC 2 cut(s) 779, 866
BmiI GGNNCC 1 cut(s) 1180
BmrFI CCNGG 7 cut(s) 291, 329, 493, 828, 936, 1184, 1393
BmsI GCATC 5 cut(s) 412, 610, 695, 1145, 1255
BmtI GCTAGC 1 cut(s) 1308
BpiI GAAGAC 3 cut(s) 891, 1200, 1286
BpmI CTGGAG 1 cut(s) 780
Bpu1102I GCTNAGC 1 cut(s) 270
BpuMI CCSGG 3 cut(s) 329, 493, 1184
BsaAI YACGTR 1 cut(s) 219
BsaBI GATNNNNATC 1 cut(s) 696
BsaHI GRCGYC 1 cut(s) 738
BsaI GGTCTC 2 cut(s) 196, 1204
BsaJI CCNNGG 9 cut(s) 235, 261, 343, 514, 527, 934, 1063, 1117, 1182
BsaWI WCCGGW 2 cut(s) 751, 1319
Bse118I RCCGGY 1 cut(s) 751
Bse1I ACTGG 3 cut(s) 170, 877, 1135
Bse3DI GCAATG 1 cut(s) 436
Bse8I GATNNNNATC 1 cut(s) 696
BseBI CCWGG 4 cut(s) 291, 828, 936, 1393
BseDI CCNNGG 9 cut(s) 235, 261, 343, 514, 527, 934, 1063, 1117, 1182
BseGI GGATG 4 cut(s) 231, 686, 837, 1316
BseJI GATNNNNATC 1 cut(s) 696
BseMI GCAATG 1 cut(s) 436
BseMII CTCAG 3 cut(s) 261, 1019, 1244
BseNI ACTGG 3 cut(s) 170, 877, 1135
BseRI GAGGAG 1 cut(s) 907
BseSI GKGCMC 1 cut(s) 1183
BseXI GCAGC 5 cut(s) 286, 298, 653, 748, 935
BseYI CCCAGC 1 cut(s) 845
BsgI GTGCAG 1 cut(s) 478
Bsh1236I CGCG 1 cut(s) 140
BshFI GGCC 4 cut(s) 392, 651, 939, 1116
BshNI GGYRCC 1 cut(s) 1178
BshTI ACCGGT 1 cut(s) 751
BsiSI CCGG 7 cut(s) 311, 329, 479, 492, 752, 1184, 1320
BslFI GGGAC 3 cut(s) 112, 335, 447
BsmAI GTCTC 2 cut(s) 196, 1204
BsmFI GGGAC 3 cut(s) 112, 335, 447
BsnI GGCC 4 cut(s) 392, 651, 939, 1116
Bso31I GGTCTC 2 cut(s) 196, 1204
Bsp1286I GDGCHC 1 cut(s) 1183
Bsp143I GATC 4 cut(s) 451, 949, 1008, 1220
Bsp1720I GCTNAGC 1 cut(s) 270
Bsp19I CCATGG 1 cut(s) 235
BspACI CCGC 3 cut(s) 138, 188, 999
BspANI GGCC 4 cut(s) 392, 651, 939, 1116
BspCNI CTCAG 3 cut(s) 262, 1018, 1245
BspFNI CGCG 1 cut(s) 140
BspLI GGNNCC 1 cut(s) 1180
BspOI GCTAGC 1 cut(s) 1308
BspQI GCTCTTC 1 cut(s) 600
BspT107I GGYRCC 1 cut(s) 1178
BspTNI GGTCTC 2 cut(s) 196, 1204
BsrDI GCAATG 1 cut(s) 436
BsrFI RCCGGY 1 cut(s) 751
BsrI ACTGG 3 cut(s) 170, 877, 1135
BssAI RCCGGY 1 cut(s) 751
BssECI CCNNGG 9 cut(s) 235, 261, 343, 514, 527, 934, 1063, 1117, 1182
BssMI GATC 4 cut(s) 451, 949, 1008, 1220
BssNI GRCGYC 1 cut(s) 738
BssT1I CCWWGG 3 cut(s) 235, 343, 1117
Bst2UI CCWGG 4 cut(s) 291, 828, 936, 1393
Bst4CI ACNGT 4 cut(s) 65, 229, 470, 1291
Bst6I CTCTTC 2 cut(s) 75, 600
BstACI GRCGYC 1 cut(s) 738
BstAPI GCANNNNNTGC 1 cut(s) 488
BstBAI YACGTR 1 cut(s) 219
BstC8I GCNNGC 5 cut(s) 138, 427, 436, 1306, 1330
BstDEI CTNAG 5 cut(s) 270, 971, 1005, 1023, 1253
BstDSI CCRYGG 1 cut(s) 235
BstF5I GGATG 4 cut(s) 231, 686, 837, 1316
BstFNI CGCG 1 cut(s) 140
BstHHI GCGC 1 cut(s) 860
BstKTI GATC 4 cut(s) 454, 952, 1011, 1223
BstMAI GTCTC 2 cut(s) 196, 1204
BstMBI GATC 4 cut(s) 451, 949, 1008, 1220
BstMWI GCNNNNNNNGC 7 cut(s) 266, 283, 431, 435, 488, 665, 1338
BstNI CCWGG 4 cut(s) 291, 828, 936, 1393
BstNSI RCATGY 2 cut(s) 95, 211
BstSCI CCNGG 7 cut(s) 289, 327, 491, 826, 934, 1182, 1391
BstSFI CTRYAG 1 cut(s) 636
BstSLI GKGCMC 1 cut(s) 1183
BstUI CGCG 1 cut(s) 140
BstV1I GCAGC 5 cut(s) 286, 298, 653, 748, 935
BstV2I GAAGAC 3 cut(s) 891, 1200, 1286
BstXI CCANNNNNNTGG 2 cut(s) 659, 1124
BsuRI GGCC 4 cut(s) 392, 651, 939, 1116
BtgI CCRYGG 1 cut(s) 235
BtsCI GGATG 4 cut(s) 231, 686, 837, 1316
BtsIMutI CAGTG 6 cut(s) 924, 1211, 1230, 1250, 1267, 1287
Cac8I GCNNGC 5 cut(s) 138, 427, 436, 1306, 1330
CfoI GCGC 1 cut(s) 860
Cfr10I RCCGGY 1 cut(s) 751
Cfr13I GGNCC 2 cut(s) 779, 866
CseI GACGC 2 cut(s) 674, 727
Csp6I GTAC 7 cut(s) 106, 169, 220, 803, 1128, 1153, 1323
CspAI ACCGGT 1 cut(s) 751
CviAII CATG 5 cut(s) 92, 118, 158, 208, 236
CviQI GTAC 7 cut(s) 106, 169, 220, 803, 1128, 1153, 1323
DdeI CTNAG 5 cut(s) 270, 971, 1005, 1023, 1253
DpnI GATC 4 cut(s) 453, 951, 1010, 1222
DpnII GATC 4 cut(s) 451, 949, 1008, 1220
DraI TTTAAA 1 cut(s) 1149
DrdI GACNNNNNNGTC 1 cut(s) 158
DseDI GACNNNNNNGTC 1 cut(s) 158
EaeI YGGCCR 1 cut(s) 649
Eam1104I CTCTTC 2 cut(s) 75, 600
EarI CTCTTC 2 cut(s) 75, 600
Eco130I CCWWGG 3 cut(s) 235, 343, 1117
Eco31I GGTCTC 2 cut(s) 196, 1204
Eco47I GGWCC 2 cut(s) 779, 866
Eco57I CTGAAG 2 cut(s) 903, 1095
EcoRI GAATTC 1 cut(s) 294
EcoRII CCWGG 4 cut(s) 289, 826, 934, 1391
EcoT14I CCWWGG 3 cut(s) 235, 343, 1117
EcoT22I ATGCAT 1 cut(s) 625
ErhI CCWWGG 3 cut(s) 235, 343, 1117
FaeI CATG 5 cut(s) 95, 121, 161, 211, 239
FalI AAGNNNNNCTT 2 cut(s) 1016, 1048
FaqI GGGAC 3 cut(s) 112, 335, 447
FatI CATG 5 cut(s) 91, 117, 157, 207, 235
FauI CCCGC 1 cut(s) 145
FauNDI CATATG 1 cut(s) 598
FblI GTMKAC 1 cut(s) 945
Fnu4HI GCNGC 5 cut(s) 275, 287, 642, 762, 924
FokI GGATG 4 cut(s) 218, 673, 824, 1303
Fsp4HI GCNGC 5 cut(s) 275, 287, 642, 762, 924
FspBI CTAG 5 cut(s) 399, 774, 842, 1100, 1305
GlaI GCGC 1 cut(s) 859
GluI GCNGC 5 cut(s) 275, 287, 642, 762, 924
GsaI CCCAGC 1 cut(s) 849
GsuI CTGGAG 1 cut(s) 780
HaeIII GGCC 4 cut(s) 392, 651, 939, 1116
HapII CCGG 7 cut(s) 311, 329, 479, 492, 752, 1184, 1320
HgaI GACGC 2 cut(s) 674, 727
HhaI GCGC 1 cut(s) 860
Hin1I GRCGYC 1 cut(s) 738
Hin1II CATG 5 cut(s) 95, 121, 161, 211, 239
Hin6I GCGC 1 cut(s) 858
HinP1I GCGC 1 cut(s) 858
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 8 cut(s) 197, 409, 475, 541, 692, 1043, 1162, 1247
HpaII CCGG 7 cut(s) 311, 329, 479, 492, 752, 1184, 1320
HphI GGTGA 6 cut(s) 461, 562, 614, 674, 741, 1027
Hpy166II GTNNAC 4 cut(s) 163, 852, 946, 1155
Hpy188I TCNGA 8 cut(s) 196, 474, 528, 691, 1008, 1067, 1167, 1354
Hpy188III TCNNGA 6 cut(s) 464, 774, 797, 881, 929, 1100
Hpy8I GTNNAC 4 cut(s) 163, 852, 946, 1155
Hpy99I CGWCG 3 cut(s) 152, 155, 181
HpyAV CCTTC 3 cut(s) 756, 1021, 1096
HpyCH4III ACNGT 4 cut(s) 65, 229, 470, 1291
HpyCH4IV ACGT 2 cut(s) 104, 218
HpyCH4V TGCA 5 cut(s) 353, 459, 623, 686, 1136
HpyF10VI GCNNNNNNNGC 7 cut(s) 266, 283, 431, 435, 488, 665, 1338
HpyF3I CTNAG 5 cut(s) 270, 971, 1005, 1023, 1253
HpySE526I ACGT 2 cut(s) 104, 218
Hsp92I GRCGYC 1 cut(s) 738
Hsp92II CATG 5 cut(s) 95, 121, 161, 211, 239
HspAI GCGC 1 cut(s) 858
Kzo9I GATC 4 cut(s) 451, 949, 1008, 1220
LguI GCTCTTC 1 cut(s) 600
Lsp1109I GCAGC 5 cut(s) 286, 298, 653, 748, 935
LweI GCATC 5 cut(s) 412, 610, 695, 1145, 1255
MaeI CTAG 5 cut(s) 399, 774, 842, 1100, 1305
MaeII ACGT 2 cut(s) 104, 218
MaeIII GTNAC 5 cut(s) 31, 509, 662, 790, 1291
MalI GATC 4 cut(s) 453, 951, 1010, 1222
MboI GATC 4 cut(s) 451, 949, 1008, 1220
MhlI GDGCHC 1 cut(s) 1183
MlsI TGGCCA 1 cut(s) 651
MluCI AATT 6 cut(s) 294, 359, 711, 784, 991, 1075
MluNI TGGCCA 1 cut(s) 651
MlyI GAGTC 2 cut(s) 550, 1156
MmeI TCCRAC 3 cut(s) 725, 766, 844
Mox20I TGGCCA 1 cut(s) 651
Mph1103I ATGCAT 1 cut(s) 625
MroXI GAANNNNTTC 1 cut(s) 1031
MscI TGGCCA 1 cut(s) 651
MseI TTAA 1 cut(s) 1148
MslI CAYNNNNRTG 3 cut(s) 63, 116, 917
Msp20I TGGCCA 1 cut(s) 651
MspA1I CMGCKG 1 cut(s) 203
MspI CCGG 7 cut(s) 311, 329, 479, 492, 752, 1184, 1320
MspR9I CCNGG 7 cut(s) 291, 329, 493, 828, 936, 1184, 1393
MvaI CCWGG 4 cut(s) 291, 828, 936, 1393
MvnI CGCG 1 cut(s) 140
MwoI GCNNNNNNNGC 7 cut(s) 266, 283, 431, 435, 488, 665, 1338
NciI CCSGG 3 cut(s) 329, 493, 1184
NcoI CCATGG 1 cut(s) 235
NdeI CATATG 1 cut(s) 598
NdeII GATC 4 cut(s) 451, 949, 1008, 1220
NheI GCTAGC 1 cut(s) 1304
NlaIII CATG 5 cut(s) 95, 121, 161, 211, 239
NlaIV GGNNCC 1 cut(s) 1180
NmeAIII GCCGAG 1 cut(s) 286
NmuCI GTSAC 4 cut(s) 509, 662, 790, 1291
NsiI ATGCAT 1 cut(s) 625
NspI RCATGY 2 cut(s) 95, 211
OliI CACNNNNGTG 1 cut(s) 63
PciSI GCTCTTC 1 cut(s) 600
PdmI GAANNNNTTC 1 cut(s) 1031
PfeI GAWTC 6 cut(s) 197, 409, 475, 692, 1043, 1247
PflFI GACNNNGTC 1 cut(s) 158
PinAI ACCGGT 1 cut(s) 751
PkrI GCNGC 5 cut(s) 276, 288, 643, 763, 925
PleI GAGTC 2 cut(s) 549, 1156
PpsI GAGTC 2 cut(s) 549, 1156
Ppu21I YACGTR 1 cut(s) 219
Psp6I CCWGG 4 cut(s) 289, 826, 934, 1391
PspFI CCCAGC 1 cut(s) 845
PspGI CCWGG 4 cut(s) 289, 826, 934, 1391
PspN4I GGNNCC 1 cut(s) 1180
PspPI GGNCC 2 cut(s) 779, 866
PsyI GACNNNGTC 1 cut(s) 158
PvuII CAGCTG 1 cut(s) 203
RsaI GTAC 7 cut(s) 107, 170, 221, 804, 1129, 1154, 1324
RsaNI GTAC 7 cut(s) 106, 169, 220, 803, 1128, 1153, 1323
RseI CAYNNNNRTG 3 cut(s) 63, 116, 917
SapI GCTCTTC 1 cut(s) 600
SaqAI TTAA 1 cut(s) 1148
SatI GCNGC 5 cut(s) 275, 287, 642, 762, 924
Sau3AI GATC 4 cut(s) 451, 949, 1008, 1220
Sau96I GGNCC 2 cut(s) 779, 866
ScaI AGTACT 1 cut(s) 804
SchI GAGTC 2 cut(s) 550, 1156
ScrFI CCNGG 7 cut(s) 291, 329, 493, 828, 936, 1184, 1393
SduI GDGCHC 1 cut(s) 1183
SfaNI GCATC 5 cut(s) 412, 610, 695, 1145, 1255
SfcI CTRYAG 1 cut(s) 636
SgrAI CRCCGGYG 1 cut(s) 751
SinI GGWCC 2 cut(s) 779, 866
SmiMI CAYNNNNRTG 3 cut(s) 63, 116, 917
Sse9I AATT 6 cut(s) 294, 359, 711, 784, 991, 1075
SsiI CCGC 3 cut(s) 138, 188, 999
SspMI CTAG 5 cut(s) 399, 774, 842, 1100, 1305
StyD4I CCNGG 7 cut(s) 289, 327, 491, 826, 934, 1182, 1391
StyI CCWWGG 3 cut(s) 235, 343, 1117
TaaI ACNGT 4 cut(s) 65, 229, 470, 1291
TaiI ACGT 2 cut(s) 107, 221
TaqI TCGA 3 cut(s) 147, 279, 443
TaqII GACCGA 1 cut(s) 528
TasI AATT 6 cut(s) 294, 359, 711, 784, 991, 1075
TatI WGTACW 2 cut(s) 802, 1152
TfiI GAWTC 6 cut(s) 197, 409, 475, 692, 1043, 1247
Tru1I TTAA 1 cut(s) 1148
Tru9I TTAA 1 cut(s) 1148
TscAI CASTG 6 cut(s) 924, 1218, 1230, 1257, 1267, 1294
TseFI GTSAC 4 cut(s) 509, 662, 790, 1291
TseI GCWGC 5 cut(s) 274, 286, 641, 761, 923
Tsp45I GTSAC 4 cut(s) 509, 662, 790, 1291
TspDTI ATGAA 2 cut(s) 17, 848
TspGWI ACGGA 3 cut(s) 10, 743, 1225
TspRI CASTG 6 cut(s) 924, 1218, 1230, 1257, 1267, 1294
Tth111I GACNNNGTC 1 cut(s) 158
VpaK11BI GGWCC 2 cut(s) 779, 866
XapI RAATTY 3 cut(s) 294, 359, 784
XbaI TCTAGA 2 cut(s) 773, 1099
XceI RCATGY 2 cut(s) 95, 211
XmiI GTMKAC 1 cut(s) 945
XmnI GAANNNNTTC 1 cut(s) 1031
XspI CTAG 5 cut(s) 399, 774, 842, 1100, 1305
ZrmI AGTACT 1 cut(s) 804
Zsp2I ATGCAT 1 cut(s) 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.