subtilisin-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 4 cut(s) | 42, 63, 462, 864 |
| AatII | GACGTC | 1 cut(s) | 40 |
| AccB1I | GGYRCC | 1 cut(s) | 186 |
| AccII | CGCG | 9 cut(s) | 300, 342, 354, 356, 585, 597, 642, 658, 721 |
| AccIII | TCCGGA | 1 cut(s) | 96 |
| AclI | AACGTT | 1 cut(s) | 469 |
| AclWI | GGATC | 8 cut(s) | 343, 356, 387, 411, 539, 564, 633, 661 |
| AcoI | YGGCCR | 7 cut(s) | 28, 69, 272, 364, 436, 574, 961 |
| AcvI | CACGTG | 1 cut(s) | 133 |
| AcyI | GRCGYC | 2 cut(s) | 37, 753 |
| AfaI | GTAC | 3 cut(s) | 195, 339, 600 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 20, 304, 358 |
| AflIII | ACRYGT | 1 cut(s) | 132 |
| AgsI | TTSAA | 1 cut(s) | 155 |
| AjnI | CCWGG | 3 cut(s) | 189, 249, 375 |
| AluBI | AGCT | 7 cut(s) | 8, 85, 121, 151, 179, 421, 956 |
| AluI | AGCT | 7 cut(s) | 8, 85, 121, 151, 179, 421, 956 |
| Alw21I | GWGCWC | 1 cut(s) | 573 |
| AlwI | GGATC | 8 cut(s) | 343, 356, 387, 411, 539, 564, 633, 661 |
| Ama87I | CYCGRG | 1 cut(s) | 874 |
| Aor13HI | TCCGGA | 1 cut(s) | 96 |
| AspLEI | GCGC | 4 cut(s) | 344, 356, 597, 642 |
| AspS9I | GGNCC | 7 cut(s) | 105, 225, 253, 307, 588, 878, 923 |
| AsuC2I | CCSGG | 5 cut(s) | 346, 666, 794, 875, 876 |
| AsuHPI | GGTGA | 2 cut(s) | 308, 845 |
| AvaI | CYCGRG | 1 cut(s) | 874 |
| AvaII | GGWCC | 6 cut(s) | 105, 253, 307, 588, 878, 923 |
| BaeGI | GKGCMC | 2 cut(s) | 132, 648 |
| BalI | TGGCCA | 3 cut(s) | 71, 366, 438 |
| BamHI | GGATCC | 1 cut(s) | 348 |
| BanI | GGYRCC | 1 cut(s) | 186 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 773, 805 |
| BauI | CACGAG | 2 cut(s) | 146, 566 |
| BbrPI | CACGTG | 1 cut(s) | 133 |
| Bbv12I | GWGCWC | 1 cut(s) | 573 |
| BbvCI | CCTCAGC | 1 cut(s) | 675 |
| BccI | CCATC | 6 cut(s) | 237, 356, 428, 459, 539, 803 |
| BceAI | ACGGC | 2 cut(s) | 287, 962 |
| BciT130I | CCWGG | 3 cut(s) | 191, 251, 377 |
| BcnI | CCSGG | 5 cut(s) | 346, 666, 794, 875, 876 |
| BisI | GCNGC | 5 cut(s) | 301, 385, 491, 904, 940 |
| BlsI | GCNGC | 5 cut(s) | 302, 386, 492, 905, 941 |
| Bme1390I | CCNGG | 8 cut(s) | 191, 251, 346, 377, 666, 794, 875, 876 |
| Bme18I | GGWCC | 6 cut(s) | 105, 253, 307, 588, 878, 923 |
| BmeT110I | CYCGRG | 1 cut(s) | 874 |
| BmgT120I | GGNCC | 7 cut(s) | 105, 225, 253, 307, 588, 878, 923 |
| BmrFI | CCNGG | 8 cut(s) | 191, 251, 346, 377, 666, 794, 875, 876 |
| BpmI | CTGGAG | 1 cut(s) | 82 |
| Bpu10I | CCTNAGC | 1 cut(s) | 675 |
| BpuMI | CCSGG | 5 cut(s) | 346, 666, 794, 875, 876 |
| BsaAI | YACGTR | 1 cut(s) | 133 |
| BsaBI | GATNNNNATC | 1 cut(s) | 561 |
| BsaHI | GRCGYC | 2 cut(s) | 37, 753 |
| BsaWI | WCCGGW | 2 cut(s) | 96, 318 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 237, 267 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 20, 304, 358 |
| Bse118I | RCCGGY | 1 cut(s) | 768 |
| Bse1I | ACTGG | 4 cut(s) | 31, 65, 655, 778 |
| Bse8I | GATNNNNATC | 1 cut(s) | 561 |
| BseAI | TCCGGA | 1 cut(s) | 96 |
| BseBI | CCWGG | 3 cut(s) | 191, 251, 377 |
| BseGI | GGATG | 1 cut(s) | 367 |
| BseJI | GATNNNNATC | 1 cut(s) | 561 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 20, 304, 358 |
| BseMII | CTCAG | 2 cut(s) | 689, 715 |
| BseNI | ACTGG | 4 cut(s) | 31, 65, 655, 778 |
| BseRI | GAGGAG | 2 cut(s) | 149, 966 |
| BseSI | GKGCMC | 2 cut(s) | 132, 648 |
| BseX3I | CGGCCG | 1 cut(s) | 574 |
| BseYI | CCCAGC | 1 cut(s) | 591 |
| Bsh1236I | CGCG | 9 cut(s) | 300, 342, 354, 356, 585, 597, 642, 658, 721 |
| Bsh1285I | CGRYCG | 3 cut(s) | 529, 577, 860 |
| BshNI | GGYRCC | 1 cut(s) | 186 |
| BsiEI | CGRYCG | 3 cut(s) | 529, 577, 860 |
| BsiHKAI | GWGCWC | 1 cut(s) | 573 |
| BsiHKCI | CYCGRG | 1 cut(s) | 874 |
| BslFI | GGGAC | 3 cut(s) | 239, 528, 574 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 20, 304, 358 |
| BsmFI | GGGAC | 3 cut(s) | 239, 528, 574 |
| BsoBI | CYCGRG | 1 cut(s) | 874 |
| Bsp1286I | GDGCHC | 3 cut(s) | 132, 573, 648 |
| Bsp13I | TCCGGA | 1 cut(s) | 96 |
| Bsp143I | GATC | 9 cut(s) | 348, 379, 403, 526, 531, 556, 562, 625, 653 |
| Bsp68I | TCGCGA | 1 cut(s) | 658 |
| BspCNI | CTCAG | 2 cut(s) | 688, 714 |
| BspEI | TCCGGA | 1 cut(s) | 96 |
| BspFNI | CGCG | 9 cut(s) | 300, 342, 354, 356, 585, 597, 642, 658, 721 |
| BspPI | GGATC | 8 cut(s) | 343, 356, 387, 411, 539, 564, 633, 661 |
| BspQI | GCTCTTC | 1 cut(s) | 15 |
| BspT107I | GGYRCC | 1 cut(s) | 186 |
| BsrFI | RCCGGY | 1 cut(s) | 768 |
| BsrI | ACTGG | 4 cut(s) | 31, 65, 655, 778 |
| BssAI | RCCGGY | 1 cut(s) | 768 |
| BssMI | GATC | 9 cut(s) | 348, 379, 403, 526, 531, 556, 562, 625, 653 |
| BssNI | GRCGYC | 2 cut(s) | 37, 753 |
| BssSI | CACGAG | 2 cut(s) | 146, 566 |
| BssT1I | CCWWGG | 1 cut(s) | 241 |
| Bst2BI | CACGAG | 2 cut(s) | 146, 566 |
| Bst2UI | CCWGG | 3 cut(s) | 191, 251, 377 |
| Bst4CI | ACNGT | 5 cut(s) | 603, 635, 690, 861, 893 |
| Bst6I | CTCTTC | 1 cut(s) | 15 |
| BstACI | GRCGYC | 2 cut(s) | 37, 753 |
| BstBAI | YACGTR | 1 cut(s) | 133 |
| BstC8I | GCNNGC | 4 cut(s) | 119, 326, 373, 644 |
| BstDEI | CTNAG | 2 cut(s) | 675, 701 |
| BstDSI | CCRYGG | 6 cut(s) | 275, 298, 621, 719, 860, 892 |
| BstF5I | GGATG | 1 cut(s) | 367 |
| BstFNI | CGCG | 9 cut(s) | 300, 342, 354, 356, 585, 597, 642, 658, 721 |
| BstHHI | GCGC | 4 cut(s) | 344, 356, 597, 642 |
| BstKTI | GATC | 9 cut(s) | 351, 382, 406, 529, 534, 559, 565, 628, 656 |
| BstMBI | GATC | 9 cut(s) | 348, 379, 403, 526, 531, 556, 562, 625, 653 |
| BstMCI | CGRYCG | 3 cut(s) | 529, 577, 860 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 127, 297, 372, 381, 406, 582, 774 |
| BstNI | CCWGG | 3 cut(s) | 191, 251, 377 |
| BstSCI | CCNGG | 8 cut(s) | 189, 249, 344, 375, 664, 792, 873, 874 |
| BstSLI | GKGCMC | 2 cut(s) | 132, 648 |
| BstUI | CGCG | 9 cut(s) | 300, 342, 354, 356, 585, 597, 642, 658, 721 |
| BstX2I | RGATCY | 3 cut(s) | 348, 531, 556 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 743 |
| BstYI | RGATCY | 3 cut(s) | 348, 531, 556 |
| BstZI | CGGCCG | 1 cut(s) | 574 |
| BtgI | CCRYGG | 6 cut(s) | 275, 298, 621, 719, 860, 892 |
| BtgZI | GCGATG | 1 cut(s) | 558 |
| BtsCI | GGATG | 1 cut(s) | 367 |
| BtsIMutI | CAGTG | 3 cut(s) | 38, 58, 648 |
| BtuMI | TCGCGA | 1 cut(s) | 658 |
| Cac8I | GCNNGC | 4 cut(s) | 119, 326, 373, 644 |
| CfoI | GCGC | 4 cut(s) | 344, 356, 597, 642 |
| Cfr10I | RCCGGY | 1 cut(s) | 768 |
| Cfr13I | GGNCC | 7 cut(s) | 105, 225, 253, 307, 588, 878, 923 |
| Cfr42I | CCGCGG | 2 cut(s) | 301, 722 |
| Cfr9I | CCCGGG | 1 cut(s) | 874 |
| CseI | GACGC | 1 cut(s) | 761 |
| Csp6I | GTAC | 3 cut(s) | 194, 338, 599 |
| CviAII | CATG | 3 cut(s) | 479, 806, 920 |
| CviQI | GTAC | 3 cut(s) | 194, 338, 599 |
| DdeI | CTNAG | 2 cut(s) | 675, 701 |
| DpnI | GATC | 9 cut(s) | 350, 381, 405, 528, 533, 558, 564, 627, 655 |
| DpnII | GATC | 9 cut(s) | 348, 379, 403, 526, 531, 556, 562, 625, 653 |
| DrdI | GACNNNNNNGTC | 4 cut(s) | 42, 63, 462, 864 |
| DseDI | GACNNNNNNGTC | 4 cut(s) | 42, 63, 462, 864 |
| EaeI | YGGCCR | 7 cut(s) | 28, 69, 272, 364, 436, 574, 961 |
| EagI | CGGCCG | 1 cut(s) | 574 |
| Eam1104I | CTCTTC | 1 cut(s) | 15 |
| EarI | CTCTTC | 1 cut(s) | 15 |
| EciI | GGCGGA | 1 cut(s) | 508 |
| EclXI | CGGCCG | 1 cut(s) | 574 |
| Eco130I | CCWWGG | 1 cut(s) | 241 |
| Eco47I | GGWCC | 6 cut(s) | 105, 253, 307, 588, 878, 923 |
| Eco52I | CGGCCG | 1 cut(s) | 574 |
| Eco72I | CACGTG | 1 cut(s) | 133 |
| Eco88I | CYCGRG | 1 cut(s) | 874 |
| EcoO109I | RGGNCCY | 3 cut(s) | 225, 253, 588 |
| EcoRII | CCWGG | 3 cut(s) | 189, 249, 375 |
| EcoT14I | CCWWGG | 1 cut(s) | 241 |
| ErhI | CCWWGG | 1 cut(s) | 241 |
| FaeI | CATG | 3 cut(s) | 482, 809, 923 |
| FaiI | YATR | 4 cut(s) | 441, 480, 807, 921 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 543, 575 |
| FaqI | GGGAC | 3 cut(s) | 239, 528, 574 |
| FatI | CATG | 3 cut(s) | 478, 805, 919 |
| FauI | CCCGC | 4 cut(s) | 296, 402, 578, 635 |
| Fnu4HI | GCNGC | 5 cut(s) | 301, 385, 491, 904, 940 |
| FokI | GGATG | 1 cut(s) | 374 |
| Fsp4HI | GCNGC | 5 cut(s) | 301, 385, 491, 904, 940 |
| GlaI | GCGC | 4 cut(s) | 343, 355, 596, 641 |
| GluI | GCNGC | 5 cut(s) | 301, 385, 491, 904, 940 |
| GsaI | CCCAGC | 1 cut(s) | 595 |
| GsuI | CTGGAG | 1 cut(s) | 82 |
| HgaI | GACGC | 1 cut(s) | 761 |
| HhaI | GCGC | 4 cut(s) | 344, 356, 597, 642 |
| Hin1I | GRCGYC | 2 cut(s) | 37, 753 |
| Hin1II | CATG | 3 cut(s) | 482, 809, 923 |
| Hin6I | GCGC | 4 cut(s) | 342, 354, 595, 640 |
| HinP1I | GCGC | 4 cut(s) | 342, 354, 595, 640 |
| HincII | GTYRAC | 2 cut(s) | 467, 869 |
| HindII | GTYRAC | 2 cut(s) | 467, 869 |
| HinfI | GANTC | 7 cut(s) | 64, 93, 100, 246, 797, 865, 913 |
| HphI | GGTGA | 2 cut(s) | 308, 845 |
| Hpy166II | GTNNAC | 2 cut(s) | 467, 869 |
| Hpy188I | TCNGA | 9 cut(s) | 48, 105, 175, 425, 503, 531, 684, 731, 820 |
| Hpy188III | TCNNGA | 5 cut(s) | 97, 431, 560, 657, 881 |
| Hpy8I | GTNNAC | 2 cut(s) | 467, 869 |
| Hpy99I | CGWCG | 5 cut(s) | 48, 273, 315, 459, 969 |
| HpyAV | CCTTC | 3 cut(s) | 119, 545, 830 |
| HpyCH4III | ACNGT | 5 cut(s) | 603, 635, 690, 861, 893 |
| HpyCH4IV | ACGT | 4 cut(s) | 37, 132, 463, 469 |
| HpyCH4V | TGCA | 1 cut(s) | 805 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 127, 297, 372, 381, 406, 582, 774 |
| HpyF3I | CTNAG | 2 cut(s) | 675, 701 |
| HpySE526I | ACGT | 4 cut(s) | 37, 132, 463, 469 |
| Hsp92I | GRCGYC | 2 cut(s) | 37, 753 |
| Hsp92II | CATG | 3 cut(s) | 482, 809, 923 |
| HspAI | GCGC | 4 cut(s) | 342, 354, 595, 640 |
| KflI | GGGWCCC | 1 cut(s) | 588 |
| Kpn2I | TCCGGA | 1 cut(s) | 96 |
| KspI | CCGCGG | 2 cut(s) | 301, 722 |
| Kzo9I | GATC | 9 cut(s) | 348, 379, 403, 526, 531, 556, 562, 625, 653 |
| LguI | GCTCTTC | 1 cut(s) | 15 |
| LmnI | GCTCC | 4 cut(s) | 321, 426, 623, 953 |
| MaeII | ACGT | 4 cut(s) | 37, 132, 463, 469 |
| MaeIII | GTNAC | 4 cut(s) | 33, 314, 771, 780 |
| MalI | GATC | 9 cut(s) | 350, 381, 405, 528, 533, 558, 564, 627, 655 |
| MboI | GATC | 9 cut(s) | 348, 379, 403, 526, 531, 556, 562, 625, 653 |
| MflI | RGATCY | 3 cut(s) | 348, 531, 556 |
| MhlI | GDGCHC | 3 cut(s) | 132, 573, 648 |
| MlsI | TGGCCA | 3 cut(s) | 71, 366, 438 |
| MluCI | AATT | 1 cut(s) | 725 |
| MluNI | TGGCCA | 3 cut(s) | 71, 366, 438 |
| MlyI | GAGTC | 5 cut(s) | 73, 87, 94, 255, 874 |
| MmeI | TCCRAC | 6 cut(s) | 26, 34, 448, 526, 634, 955 |
| MnlI | CCTC | 8 cut(s) | 170, 206, 684, 690, 725, 903, 936, 944 |
| Mox20I | TGGCCA | 3 cut(s) | 71, 366, 438 |
| MroI | TCCGGA | 1 cut(s) | 96 |
| MscI | TGGCCA | 3 cut(s) | 71, 366, 438 |
| MslI | CAYNNNNRTG | 1 cut(s) | 432 |
| Msp20I | TGGCCA | 3 cut(s) | 71, 366, 438 |
| MspA1I | CMGCKG | 2 cut(s) | 300, 721 |
| MspR9I | CCNGG | 8 cut(s) | 191, 251, 346, 377, 666, 794, 875, 876 |
| MvaI | CCWGG | 3 cut(s) | 191, 251, 377 |
| MvnI | CGCG | 9 cut(s) | 300, 342, 354, 356, 585, 597, 642, 658, 721 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 127, 297, 372, 381, 406, 582, 774 |
| NciI | CCSGG | 5 cut(s) | 346, 666, 794, 875, 876 |
| NdeII | GATC | 9 cut(s) | 348, 379, 403, 526, 531, 556, 562, 625, 653 |
| NlaIII | CATG | 3 cut(s) | 482, 809, 923 |
| NmeAIII | GCCGAG | 5 cut(s) | 141, 466, 519, 552, 594 |
| NmuCI | GTSAC | 2 cut(s) | 33, 314 |
| NruI | TCGCGA | 1 cut(s) | 658 |
| PciSI | GCTCTTC | 1 cut(s) | 15 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 460 |
| PfeI | GAWTC | 2 cut(s) | 797, 913 |
| PflFI | GACNNNGTC | 1 cut(s) | 462 |
| PfoI | TCCNGGA | 1 cut(s) | 792 |
| PkrI | GCNGC | 5 cut(s) | 302, 386, 492, 905, 941 |
| Ple19I | CGATCG | 1 cut(s) | 529 |
| PleI | GAGTC | 5 cut(s) | 72, 87, 94, 254, 873 |
| PmaCI | CACGTG | 1 cut(s) | 133 |
| PmlI | CACGTG | 1 cut(s) | 133 |
| PpsI | GAGTC | 5 cut(s) | 72, 87, 94, 254, 873 |
| Ppu21I | YACGTR | 1 cut(s) | 133 |
| PpuMI | RGGWCCY | 2 cut(s) | 253, 588 |
| Psp1406I | AACGTT | 1 cut(s) | 469 |
| Psp5II | RGGWCCY | 2 cut(s) | 253, 588 |
| Psp6I | CCWGG | 3 cut(s) | 189, 249, 375 |
| PspCI | CACGTG | 1 cut(s) | 133 |
| PspFI | CCCAGC | 1 cut(s) | 591 |
| PspGI | CCWGG | 3 cut(s) | 189, 249, 375 |
| PspPI | GGNCC | 7 cut(s) | 105, 225, 253, 307, 588, 878, 923 |
| PspPPI | RGGWCCY | 2 cut(s) | 253, 588 |
| PsuI | RGATCY | 3 cut(s) | 348, 531, 556 |
| PsyI | GACNNNGTC | 1 cut(s) | 462 |
| PvuI | CGATCG | 1 cut(s) | 529 |
| RruI | TCGCGA | 1 cut(s) | 658 |
| RsaI | GTAC | 3 cut(s) | 195, 339, 600 |
| RsaNI | GTAC | 3 cut(s) | 194, 338, 599 |
| RseI | CAYNNNNRTG | 1 cut(s) | 432 |
| SacII | CCGCGG | 2 cut(s) | 301, 722 |
| SapI | GCTCTTC | 1 cut(s) | 15 |
| SatI | GCNGC | 5 cut(s) | 301, 385, 491, 904, 940 |
| Sau3AI | GATC | 9 cut(s) | 348, 379, 403, 526, 531, 556, 562, 625, 653 |
| Sau96I | GGNCC | 7 cut(s) | 105, 225, 253, 307, 588, 878, 923 |
| SchI | GAGTC | 5 cut(s) | 73, 87, 94, 255, 874 |
| ScrFI | CCNGG | 8 cut(s) | 191, 251, 346, 377, 666, 794, 875, 876 |
| SduI | GDGCHC | 3 cut(s) | 132, 573, 648 |
| Sfr303I | CCGCGG | 2 cut(s) | 301, 722 |
| SgrBI | CCGCGG | 2 cut(s) | 301, 722 |
| SinI | GGWCC | 6 cut(s) | 105, 253, 307, 588, 878, 923 |
| SmaI | CCCGGG | 1 cut(s) | 876 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 432 |
| Sse9I | AATT | 1 cut(s) | 725 |
| StyD4I | CCNGG | 8 cut(s) | 189, 249, 344, 375, 664, 792, 873, 874 |
| StyI | CCWWGG | 1 cut(s) | 241 |
| TaaI | ACNGT | 5 cut(s) | 603, 635, 690, 861, 893 |
| TaiI | ACGT | 4 cut(s) | 40, 135, 466, 472 |
| TaqI | TCGA | 2 cut(s) | 232, 454 |
| TasI | AATT | 1 cut(s) | 725 |
| TauI | GCSGC | 5 cut(s) | 303, 387, 493, 906, 942 |
| TfiI | GAWTC | 2 cut(s) | 797, 913 |
| TscAI | CASTG | 3 cut(s) | 38, 65, 655 |
| TseFI | GTSAC | 2 cut(s) | 33, 314 |
| Tsp45I | GTSAC | 2 cut(s) | 33, 314 |
| TspGWI | ACGGA | 3 cut(s) | 292, 299, 610 |
| TspMI | CCCGGG | 1 cut(s) | 874 |
| TspRI | CASTG | 3 cut(s) | 38, 65, 655 |
| Tth111I | GACNNNGTC | 1 cut(s) | 462 |
| VpaK11BI | GGWCC | 6 cut(s) | 105, 253, 307, 588, 878, 923 |
| XmaI | CCCGGG | 1 cut(s) | 874 |
| ZraI | GACGTC | 1 cut(s) | 38 |