Rroxscaffold_7G00159530

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001634
Physical Location & Seq
Forward (+)
2718505 .. 2720412
1908 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00159530.1

Sequence Viewer

Length: 1890 bp
ATGCTTTACACGTACCAAAATGTAGTTCACGGCTTCTCCACCAGGCTTACGAGTGAGGAAGCTGAGCTGCTTGAAAAGCAGTCAGGAATTCTTTCTGTTCTTCCTGCGATGAGATATGAGCTTCATACAACTCGGACACCGGAGTTTCTTCGATTAACAGGACAATATGAAGCTTTCTTCCCAGCATCAGGCAATGTGAGTAAGGTGGTCATCGGACTTGTAGACACTGGTGTATGGCCTGCAGAGAGCAAAAGCTACAATGACAAGGGTCTTGCACCTGTGCCAAGGAGCTGGAAAGGGCAGTGCGAGGAGGTTCAGGACTTCAACTCCTCAAGCTGCAACAGGAAACTGATTGGCGCCAGGTTTTTCTCAAAAGGGTATGAGGCAGCACTTGGACCCATGAATGAAAAGTTAGAATCAAGATCACCACGAGATGATGACGGCCATGGAACTCACACCTCCAGCATTGCAGCTGGATCCCCTGTTCCAGGCGCTAGCCTCTTTGGTTACACGTCAGGGACAGCACGGGGAATGGCTGCACAAGCTCGCGTTGCCACATATATGGCATGCTGGCTTGGAGGTTGTTTCGGCTCTGATGTTCTAGCCGCAATGGACAAGGCTATTAAAGATGGTGTCAACACTTTATCCTTGTCTTTGTCTTTTCCGGGAGCACAATACGAGGATTTCTTCAGAGACATTGTTGCTATTGGAGCTTTTTCTGCCATGGCCAAGGGAGTGTTTGTGTCATGTTCAGCCGGAAATGATGGGCCAATTTCCTATACCTCATCCAACAATGCACCTTGGATAACCACTGTGGGTGCTGGAACACTAGACCAGGACTTCCCAGCTTATGTTAGTCTTGGAAATGGAAAGAAATACAGAGGTATAGCACTTGACCGCGGTACAGTATCATTATCTAGTGGATTACATCCCCTTGTTTATGCTTCTAATGCAAGTAACTCCACAAGTGGTGATCAATGCCCTCCTAACAGTTTGATTCCCGAGATGGTTGCTGGGAAAATTGTGGTATGCGATCGGGGAAGAAGCAGGGTCAGAAAGAGTGTGGTGGTAAAAAAGGCTGGTGGGGTAGGGATGATTATGGCAAACATTGAAGGTTATGGAGAAGAACTTGTAGCCGACCCATATCTCTTGCCAACTGCATTGGTTGGCAAGAAAGCTGGTGATGCAATAAAAAGATATATTGCCTCGCATGCTAATCCGGAAGCCACATTGTTGGGTATCAAACCCTCACCGGTGGTGGCAGCATTCAGCTCTAGAGGACCCAATCCAGTCGCTACATCACTACTCAAGCCAGACTTAATAGCACCGGGAGTTAATATTCTAGCTGGGTGGACTGGTGCAGATGGACCAACTGGACTACCTGAAGACAAAAAGAGGGTTAGCTACAACATCATTTCAGGTACATCCATGTCATGCGCTCATGTGACCGGTCTTGCCGCCCTTCTCAAGGCCGCTCACCCAAAATGGAGCCCTGCTGCCATTAAATCTGCTCTCATGACCACATCCTATACAAGAAGAAATGGAAAACCAATCAAAGATGTTGCTACTGGAAAGCCTGCAACACCATTTGATTATGGAGCGGGGCACGTGAACCCAGTTGGAGCCCTTGACCCTGGCCTTATTTATGATCTAGCTGTGGAGGACTACCTAGGCTTCCTCTGTGCCATGAATTACACGACAAAAGATATCAAGAAACTCACTCACAGAGACTCCACCTGTGACTCAAGAAAGAAATACAGAGTTCGAGATTTCAATTACCCATCTTTTGCTGCGACCATTTTTACCTCTGATGAAGGGGTCGGGGGTGGATCTGTCAATTACACAAGGACTCTAACCAATGTGGGCACCCCAGCAAGATACAAGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

629

Amino Acids

66.68

Weight (kDa)

8.96

Isoelectric Point (pI)

29.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 1 - 42 2.6e-07 Peptidase inhibitor I9
Peptidase_S8 PF00082 68 - 535 5.6e-39 Subtilase family
PA PF02225 311 - 397 1.3e-10 PA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 356, 1865
AccBSI CCGCTC 2 cut(s) 1475, 1601
AccI GTMKAC 1 cut(s) 222
AccII CGCG 2 cut(s) 549, 900
AccIII TCCGGA 1 cut(s) 1219
AciI CCGC 6 cut(s) 606, 898, 900, 1458, 1473, 1601
AclWI GGATC 3 cut(s) 471, 484, 1837
AcoI YGGCCR 2 cut(s) 442, 726
AcsI RAATTY 1 cut(s) 87
AcuI CTGAAG 2 cut(s) 673, 1404
AcvI CACGTG 1 cut(s) 1609
AcyI GRCGYC 1 cut(s) 357
AfaI GTAC 3 cut(s) 14, 904, 1423
AfiI CCNNNNNNNGG 3 cut(s) 188, 488, 1468
AflIII ACRYGT 2 cut(s) 9, 510
AgeI ACCGGT 2 cut(s) 1252, 1448
AgsI TTSAA 4 cut(s) 74, 325, 1112, 1774
AjiI CACGTC 1 cut(s) 513
AjnI CCWGG 5 cut(s) 41, 359, 487, 834, 1633
AjuI GAANNNNNNNTTGG 2 cut(s) 9, 41
AloI GAACNNNNNNTCC 2 cut(s) 468, 500
Alw21I GWGCWC 1 cut(s) 673
Alw26I GTCTC 2 cut(s) 687, 1722
AlwI GGATC 3 cut(s) 471, 484, 1837
Ama87I CYCGRG 1 cut(s) 1001
Aor13HI TCCGGA 1 cut(s) 1219
AoxI GGCC 6 cut(s) 236, 442, 726, 767, 1470, 1636
ApeKI GCWGC 8 cut(s) 67, 336, 386, 470, 536, 1262, 1496, 1790
ApoI RAATTY 1 cut(s) 87
AsiGI ACCGGT 2 cut(s) 1252, 1448
Asp700I GAANNNNTTC 1 cut(s) 91
AspA2I CCTAGG 1 cut(s) 1669
AspLEI GCGC 3 cut(s) 359, 494, 1439
AspS9I GGNCC 4 cut(s) 395, 767, 1280, 1367
AsuC2I CCSGG 2 cut(s) 666, 1329
AsuHPI GGTGA 5 cut(s) 417, 983, 1193, 1242, 1469
AsuNHI GCTAGC 1 cut(s) 494
AvaI CYCGRG 1 cut(s) 1001
AvaII GGWCC 3 cut(s) 395, 1280, 1367
AvrII CCTAGG 1 cut(s) 1669
BaeGI GKGCMC 2 cut(s) 1608, 1868
BalI TGGCCA 1 cut(s) 728
BamHI GGATCC 1 cut(s) 476
BanI GGYRCC 2 cut(s) 356, 1865
BanII GRGCYC 2 cut(s) 1493, 1627
BauI CACGAG 1 cut(s) 429
BbrPI CACGTG 1 cut(s) 1609
BbsI GAAGAC 1 cut(s) 1392
Bbv12I GWGCWC 1 cut(s) 673
BbvI GCAGC 8 cut(s) 54, 323, 398, 482, 523, 1274, 1483, 1777
BccI CCATC 5 cut(s) 623, 758, 1000, 1358, 1789
BceAI ACGGC 2 cut(s) 46, 457
BcgI CGANNNNNNTGC 2 cut(s) 992, 1026
BciT130I CCWGG 5 cut(s) 43, 361, 489, 836, 1635
BclI TGATCA 1 cut(s) 973
BcnI CCSGG 2 cut(s) 666, 1329
BcoDI GTCTC 2 cut(s) 687, 1722
BfaI CTAG 8 cut(s) 495, 602, 830, 918, 1275, 1343, 1652, 1670
BfmI CTRYAG 1 cut(s) 240
BfoI RGCGCY 2 cut(s) 360, 495
BlnI CCTAGG 1 cut(s) 1669
BlpI GCTNAGC 1 cut(s) 63
Bme1390I CCNGG 7 cut(s) 43, 361, 489, 666, 836, 1329, 1635
Bme18I GGWCC 3 cut(s) 395, 1280, 1367
BmeT110I CYCGRG 1 cut(s) 1001
BmgBI CACGTC 1 cut(s) 513
BmgT120I GGNCC 4 cut(s) 395, 767, 1280, 1367
BmiI GGNNCC 7 cut(s) 358, 397, 478, 1282, 1490, 1624, 1867
BmrFI CCNGG 7 cut(s) 43, 361, 489, 666, 836, 1329, 1635
BmrI ACTGGG 1 cut(s) 1610
BmsI GCATC 2 cut(s) 194, 1174
BmtI GCTAGC 1 cut(s) 498
BmuI ACTGGG 1 cut(s) 1610
BoxI GACNNNNGTC 1 cut(s) 267
BpiI GAAGAC 1 cut(s) 1392
BpmI CTGGAG 1 cut(s) 445
Bpu1102I GCTNAGC 1 cut(s) 63
BpuEI CTTGAG 4 cut(s) 316, 1292, 1451, 1729
BpuMI CCSGG 2 cut(s) 666, 1329
BsaAI YACGTR 2 cut(s) 12, 1609
BsaHI GRCGYC 1 cut(s) 357
BsaJI CCNNGG 8 cut(s) 284, 445, 723, 729, 800, 898, 1633, 1669
BsaWI WCCGGW 4 cut(s) 139, 1219, 1252, 1448
BsaXI ACNNNNNCTCC 8 cut(s) 20, 50, 311, 341, 726, 756, 1715, 1745
Bsc4I CCNNNNNNNGG 3 cut(s) 188, 488, 1468
Bse118I RCCGGY 2 cut(s) 1252, 1448
Bse1I ACTGG 6 cut(s) 232, 1289, 1360, 1378, 1573, 1616
Bse3DI GCAATG 3 cut(s) 199, 465, 615
BseAI TCCGGA 1 cut(s) 1219
BseBI CCWGG 5 cut(s) 43, 361, 489, 836, 1635
BseDI CCNNGG 8 cut(s) 284, 445, 723, 729, 800, 898, 1633, 1669
BseGI GGATG 5 cut(s) 785, 928, 1098, 1424, 1523
BseLI CCNNNNNNNGG 3 cut(s) 188, 488, 1468
BseMI GCAATG 3 cut(s) 199, 465, 615
BseMII CTCAG 1 cut(s) 54
BseNI ACTGG 6 cut(s) 232, 1289, 1360, 1378, 1573, 1616
BseRI GAGGAG 2 cut(s) 319, 323
BseSI GKGCMC 2 cut(s) 1608, 1868
BseXI GCAGC 8 cut(s) 54, 323, 398, 482, 523, 1274, 1483, 1777
BseYI CCCAGC 5 cut(s) 181, 844, 1013, 1346, 1870
BsgI GTGCAG 2 cut(s) 522, 1380
Bsh1236I CGCG 2 cut(s) 549, 900
Bsh1285I CGRYCG 1 cut(s) 1036
BshFI GGCC 6 cut(s) 238, 444, 728, 769, 1472, 1638
BshNI GGYRCC 2 cut(s) 356, 1865
BshTI ACCGGT 2 cut(s) 1252, 1448
BsiEI CGRYCG 1 cut(s) 1036
BsiHKAI GWGCWC 1 cut(s) 673
BsiHKCI CYCGRG 1 cut(s) 1001
BsiSI CCGG 7 cut(s) 140, 665, 756, 1220, 1253, 1328, 1449
BslFI GGGAC 1 cut(s) 532
BslI CCNNNNNNNGG 3 cut(s) 188, 488, 1468
BsmAI GTCTC 2 cut(s) 687, 1722
BsmFI GGGAC 1 cut(s) 532
BsmI GAATGC 1 cut(s) 1265
BsnI GGCC 6 cut(s) 238, 444, 728, 769, 1472, 1638
BsoBI CYCGRG 1 cut(s) 1001
Bsp1286I GDGCHC 5 cut(s) 673, 1493, 1608, 1627, 1868
Bsp13I TCCGGA 1 cut(s) 1219
Bsp143I GATC 6 cut(s) 422, 476, 973, 1033, 1648, 1829
Bsp1720I GCTNAGC 1 cut(s) 63
Bsp19I CCATGG 2 cut(s) 445, 723
BspACI CCGC 6 cut(s) 606, 898, 900, 1458, 1473, 1601
BspANI GGCC 6 cut(s) 238, 444, 728, 769, 1472, 1638
BspCNI CTCAG 1 cut(s) 55
BspEI TCCGGA 1 cut(s) 1219
BspFNI CGCG 2 cut(s) 549, 900
BspHI TCATGA 1 cut(s) 1515
BspLI GGNNCC 7 cut(s) 358, 397, 478, 1282, 1490, 1624, 1867
BspMAI CTGCAG 1 cut(s) 244
BspOI GCTAGC 1 cut(s) 498
BspPI GGATC 3 cut(s) 471, 484, 1837
BspT107I GGYRCC 2 cut(s) 356, 1865
BsrBI CCGCTC 2 cut(s) 1475, 1601
BsrDI GCAATG 3 cut(s) 199, 465, 615
BsrFI RCCGGY 2 cut(s) 1252, 1448
BsrI ACTGG 6 cut(s) 232, 1289, 1360, 1378, 1573, 1616
BssAI RCCGGY 2 cut(s) 1252, 1448
BssECI CCNNGG 8 cut(s) 284, 445, 723, 729, 800, 898, 1633, 1669
BssMI GATC 6 cut(s) 422, 476, 973, 1033, 1648, 1829
BssNI GRCGYC 1 cut(s) 357
BssSI CACGAG 1 cut(s) 429
BssT1I CCWWGG 6 cut(s) 284, 445, 723, 729, 800, 1669
Bst2BI CACGAG 1 cut(s) 429
Bst2UI CCWGG 5 cut(s) 43, 361, 489, 836, 1635
Bst4CI ACNGT 3 cut(s) 814, 907, 992
BstACI GRCGYC 1 cut(s) 357
BstBAI YACGTR 2 cut(s) 12, 1609
BstC8I GCNNGC 7 cut(s) 240, 496, 547, 568, 572, 1212, 1578
BstDEI CTNAG 1 cut(s) 63
BstDSI CCRYGG 3 cut(s) 445, 723, 898
BstENI CCTNNNNNAGG 2 cut(s) 486, 1466
BstF5I GGATG 5 cut(s) 785, 928, 1098, 1424, 1523
BstFNI CGCG 2 cut(s) 549, 900
BstH2I RGCGCY 2 cut(s) 360, 495
BstHHI GCGC 3 cut(s) 359, 494, 1439
BstKTI GATC 6 cut(s) 425, 479, 976, 1036, 1651, 1832
BstMAI GTCTC 2 cut(s) 687, 1722
BstMBI GATC 6 cut(s) 422, 476, 973, 1033, 1648, 1829
BstMCI CGRYCG 1 cut(s) 1036
BstMWI GCNNNNNNNGC 8 cut(s) 76, 542, 551, 710, 719, 950, 1184, 1211
BstNI CCWGG 5 cut(s) 43, 361, 489, 836, 1635
BstNSI RCATGY 2 cut(s) 570, 1214
BstPAI GACNNNNGTC 1 cut(s) 267
BstSCI CCNGG 7 cut(s) 41, 359, 487, 664, 834, 1327, 1633
BstSFI CTRYAG 1 cut(s) 240
BstSLI GKGCMC 2 cut(s) 1608, 1868
BstUI CGCG 2 cut(s) 549, 900
BstV1I GCAGC 8 cut(s) 54, 323, 398, 482, 523, 1274, 1483, 1777
BstV2I GAAGAC 1 cut(s) 1392
BstX2I RGATCY 2 cut(s) 476, 1829
BstXI CCANNNNNNTGG 3 cut(s) 291, 562, 1234
BstYI RGATCY 2 cut(s) 476, 1829
BsuRI GGCC 6 cut(s) 238, 444, 728, 769, 1472, 1638
BtgI CCRYGG 3 cut(s) 445, 723, 898
BtgZI GCGATG 1 cut(s) 122
BtrI CACGTC 1 cut(s) 513
BtsCI GGATG 5 cut(s) 785, 928, 1098, 1424, 1523
BtsI GCAGTG 1 cut(s) 308
BtsIMutI CAGTG 3 cut(s) 225, 308, 810
Cac8I GCNNGC 7 cut(s) 240, 496, 547, 568, 572, 1212, 1578
CciI TCATGA 1 cut(s) 1515
CfoI GCGC 3 cut(s) 359, 494, 1439
Cfr10I RCCGGY 2 cut(s) 1252, 1448
Cfr13I GGNCC 4 cut(s) 395, 767, 1280, 1367
Cfr42I CCGCGG 1 cut(s) 901
Csp6I GTAC 3 cut(s) 13, 903, 1422
CspAI ACCGGT 2 cut(s) 1252, 1448
CviQI GTAC 3 cut(s) 13, 903, 1422
DdeI CTNAG 1 cut(s) 63
DinI GGCGCC 1 cut(s) 358
DpnI GATC 6 cut(s) 424, 478, 975, 1035, 1650, 1831
DpnII GATC 6 cut(s) 422, 476, 973, 1033, 1648, 1829
EaeI YGGCCR 2 cut(s) 442, 726
Eco130I CCWWGG 6 cut(s) 284, 445, 723, 729, 800, 1669
Eco24I GRGCYC 2 cut(s) 1493, 1627
Eco32I GATATC 1 cut(s) 1708
Eco47I GGWCC 3 cut(s) 395, 1280, 1367
Eco57I CTGAAG 2 cut(s) 673, 1404
Eco72I CACGTG 1 cut(s) 1609
Eco88I CYCGRG 1 cut(s) 1001
EcoNI CCTNNNNNAGG 2 cut(s) 486, 1466
EcoO109I RGGNCCY 1 cut(s) 1280
EcoRI GAATTC 1 cut(s) 87
EcoRII CCWGG 5 cut(s) 41, 359, 487, 834, 1633
EcoRV GATATC 1 cut(s) 1708
EcoT14I CCWWGG 6 cut(s) 284, 445, 723, 729, 800, 1669
EcoT38I GRGCYC 2 cut(s) 1493, 1627
EgeI GGCGCC 1 cut(s) 358
EheI GGCGCC 1 cut(s) 358
ErhI CCWWGG 6 cut(s) 284, 445, 723, 729, 800, 1669
FalI AAGNNNNNCTT 2 cut(s) 1301, 1333
FaqI GGGAC 1 cut(s) 532
FauI CCCGC 1 cut(s) 1594
FbaI TGATCA 1 cut(s) 973
FblI GTMKAC 1 cut(s) 222
FokI GGATG 5 cut(s) 772, 915, 1105, 1411, 1510
FriOI GRGCYC 2 cut(s) 1493, 1627
FspBI CTAG 8 cut(s) 495, 602, 830, 918, 1275, 1343, 1652, 1670
GlaI GCGC 3 cut(s) 358, 493, 1438
GsaI CCCAGC 5 cut(s) 185, 848, 1017, 1350, 1874
GsuI CTGGAG 1 cut(s) 445
HaeII RGCGCY 2 cut(s) 360, 495
HaeIII GGCC 6 cut(s) 238, 444, 728, 769, 1472, 1638
HapII CCGG 7 cut(s) 140, 665, 756, 1220, 1253, 1328, 1449
HhaI GCGC 3 cut(s) 359, 494, 1439
Hin1I GRCGYC 1 cut(s) 357
Hin6I GCGC 3 cut(s) 357, 492, 1437
HinP1I GCGC 3 cut(s) 357, 492, 1437
HincII GTYRAC 1 cut(s) 637
HindII GTYRAC 1 cut(s) 637
HindIII AAGCTT 1 cut(s) 171
HinfI GANTC 5 cut(s) 416, 997, 1730, 1742, 1849
HpaII CCGG 7 cut(s) 140, 665, 756, 1220, 1253, 1328, 1449
HphI GGTGA 5 cut(s) 417, 983, 1193, 1242, 1469
Hpy166II GTNNAC 5 cut(s) 28, 223, 637, 1353, 1612
Hpy188I TCNGA 7 cut(s) 135, 215, 595, 692, 1055, 1810, 1889
Hpy8I GTNNAC 5 cut(s) 28, 223, 637, 1353, 1612
HpyAV CCTTC 3 cut(s) 1106, 1472, 1808
HpyCH4III ACNGT 3 cut(s) 814, 907, 992
HpyCH4IV ACGT 3 cut(s) 11, 512, 1608
HpyF10VI GCNNNNNNNGC 8 cut(s) 76, 542, 551, 710, 719, 950, 1184, 1211
HpyF3I CTNAG 1 cut(s) 63
HpySE526I ACGT 3 cut(s) 11, 512, 1608
Hsp92I GRCGYC 1 cut(s) 357
HspAI GCGC 3 cut(s) 357, 492, 1437
KasI GGCGCC 1 cut(s) 356
Kpn2I TCCGGA 1 cut(s) 1219
Ksp22I TGATCA 1 cut(s) 973
KspI CCGCGG 1 cut(s) 901
Kzo9I GATC 6 cut(s) 422, 476, 973, 1033, 1648, 1829
LmnI GCTCC 6 cut(s) 288, 668, 710, 1488, 1598, 1622
Lsp1109I GCAGC 8 cut(s) 54, 323, 398, 482, 523, 1274, 1483, 1777
LweI GCATC 2 cut(s) 194, 1174
MaeI CTAG 8 cut(s) 495, 602, 830, 918, 1275, 1343, 1652, 1670
MaeII ACGT 3 cut(s) 11, 512, 1608
MaeIII GTNAC 4 cut(s) 506, 956, 1444, 1739
MalI GATC 6 cut(s) 424, 478, 975, 1035, 1650, 1831
MbiI CCGCTC 2 cut(s) 1475, 1601
MboI GATC 6 cut(s) 422, 476, 973, 1033, 1648, 1829
MboII GAAGA 8 cut(s) 92, 140, 169, 679, 1053, 1136, 1397, 1548
MflI RGATCY 2 cut(s) 476, 1829
MhlI GDGCHC 5 cut(s) 673, 1493, 1608, 1627, 1868
MlsI TGGCCA 1 cut(s) 728
MluCI AATT 6 cut(s) 87, 771, 1020, 1690, 1774, 1837
MluNI TGGCCA 1 cut(s) 728
Mly113I GGCGCC 1 cut(s) 357
MlyI GAGTC 3 cut(s) 1724, 1736, 1843
MmeI TCCRAC 2 cut(s) 813, 1600
Mox20I TGGCCA 1 cut(s) 728
MroI TCCGGA 1 cut(s) 1219
MroXI GAANNNNTTC 1 cut(s) 91
MscI TGGCCA 1 cut(s) 728
MseI TTAA 5 cut(s) 155, 624, 1319, 1335, 1503
MslI CAYNNNNRTG 1 cut(s) 560
Msp20I TGGCCA 1 cut(s) 728
MspA1I CMGCKG 2 cut(s) 473, 900
MspI CCGG 7 cut(s) 140, 665, 756, 1220, 1253, 1328, 1449
MspR9I CCNGG 7 cut(s) 43, 361, 489, 666, 836, 1329, 1635
Mva1269I GAATGC 1 cut(s) 1265
MvaI CCWGG 5 cut(s) 43, 361, 489, 836, 1635
MvnI CGCG 2 cut(s) 549, 900
MwoI GCNNNNNNNGC 8 cut(s) 76, 542, 551, 710, 719, 950, 1184, 1211
NarI GGCGCC 1 cut(s) 357
NciI CCSGG 2 cut(s) 666, 1329
NcoI CCATGG 2 cut(s) 445, 723
NdeII GATC 6 cut(s) 422, 476, 973, 1033, 1648, 1829
NheI GCTAGC 1 cut(s) 494
NlaIV GGNNCC 7 cut(s) 358, 397, 478, 1282, 1490, 1624, 1867
NmuCI GTSAC 2 cut(s) 1444, 1739
NspI RCATGY 2 cut(s) 570, 1214
PaeI GCATGC 2 cut(s) 570, 1214
PagI TCATGA 1 cut(s) 1515
PctI GAATGC 1 cut(s) 1265
PdmI GAANNNNTTC 1 cut(s) 91
PfeI GAWTC 2 cut(s) 416, 997
PfoI TCCNGGA 1 cut(s) 664
PinAI ACCGGT 2 cut(s) 1252, 1448
Ple19I CGATCG 1 cut(s) 1036
PleI GAGTC 3 cut(s) 1724, 1736, 1843
PluTI GGCGCC 1 cut(s) 360
PmaCI CACGTG 1 cut(s) 1609
PmlI CACGTG 1 cut(s) 1609
PpsI GAGTC 3 cut(s) 1724, 1736, 1843
Ppu21I YACGTR 2 cut(s) 12, 1609
PpuMI RGGWCCY 1 cut(s) 1280
PshAI GACNNNNGTC 1 cut(s) 267
Psp5II RGGWCCY 1 cut(s) 1280
Psp6I CCWGG 5 cut(s) 41, 359, 487, 834, 1633
PspCI CACGTG 1 cut(s) 1609
PspFI CCCAGC 5 cut(s) 181, 844, 1013, 1346, 1870
PspGI CCWGG 5 cut(s) 41, 359, 487, 834, 1633
PspN4I GGNNCC 7 cut(s) 358, 397, 478, 1282, 1490, 1624, 1867
PspPI GGNCC 4 cut(s) 395, 767, 1280, 1367
PspPPI RGGWCCY 1 cut(s) 1280
PstI CTGCAG 1 cut(s) 244
PsuI RGATCY 2 cut(s) 476, 1829
PvuI CGATCG 1 cut(s) 1036
PvuII CAGCTG 1 cut(s) 473
RsaI GTAC 3 cut(s) 14, 904, 1423
RsaNI GTAC 3 cut(s) 13, 903, 1422
RseI CAYNNNNRTG 1 cut(s) 560
SacII CCGCGG 1 cut(s) 901
SaqAI TTAA 5 cut(s) 155, 624, 1319, 1335, 1503
Sau3AI GATC 6 cut(s) 422, 476, 973, 1033, 1648, 1829
Sau96I GGNCC 4 cut(s) 395, 767, 1280, 1367
SchI GAGTC 3 cut(s) 1724, 1736, 1843
ScrFI CCNGG 7 cut(s) 43, 361, 489, 666, 836, 1329, 1635
SduI GDGCHC 5 cut(s) 673, 1493, 1608, 1627, 1868
SfaNI GCATC 2 cut(s) 194, 1174
SfcI CTRYAG 1 cut(s) 240
SfoI GGCGCC 1 cut(s) 358
Sfr303I CCGCGG 1 cut(s) 901
SgrAI CRCCGGYG 1 cut(s) 1252
SgrBI CCGCGG 1 cut(s) 901
SinI GGWCC 3 cut(s) 395, 1280, 1367
SmiMI CAYNNNNRTG 1 cut(s) 560
SmlI CTYRAG 4 cut(s) 331, 1307, 1466, 1744
SmoI CTYRAG 4 cut(s) 331, 1307, 1466, 1744
SphI GCATGC 2 cut(s) 570, 1214
Sse9I AATT 6 cut(s) 87, 771, 1020, 1690, 1774, 1837
SsiI CCGC 6 cut(s) 606, 898, 900, 1458, 1473, 1601
SspDI GGCGCC 1 cut(s) 356
SspI AATATT 1 cut(s) 1339
SspMI CTAG 8 cut(s) 495, 602, 830, 918, 1275, 1343, 1652, 1670
StyD4I CCNGG 7 cut(s) 41, 359, 487, 664, 834, 1327, 1633
StyI CCWWGG 6 cut(s) 284, 445, 723, 729, 800, 1669
TaaI ACNGT 3 cut(s) 814, 907, 992
TaiI ACGT 3 cut(s) 14, 515, 1611
TaqI TCGA 2 cut(s) 151, 1765
TasI AATT 6 cut(s) 87, 771, 1020, 1690, 1774, 1837
TauI GCSGC 3 cut(s) 608, 1460, 1475
TfiI GAWTC 2 cut(s) 416, 997
Tru1I TTAA 5 cut(s) 155, 624, 1319, 1335, 1503
Tru9I TTAA 5 cut(s) 155, 624, 1319, 1335, 1503
TscAI CASTG 3 cut(s) 232, 308, 817
TseFI GTSAC 2 cut(s) 1444, 1739
TseI GCWGC 8 cut(s) 67, 336, 386, 470, 536, 1262, 1496, 1790
Tsp45I GTSAC 2 cut(s) 1444, 1739
TspDTI ATGAA 6 cut(s) 113, 183, 416, 420, 1703, 1827
TspRI CASTG 3 cut(s) 232, 308, 817
VpaK11BI GGWCC 3 cut(s) 395, 1280, 1367
XagI CCTNNNNNAGG 2 cut(s) 486, 1466
XapI RAATTY 1 cut(s) 87
XbaI TCTAGA 1 cut(s) 1274
XceI RCATGY 2 cut(s) 570, 1214
XmaJI CCTAGG 1 cut(s) 1669
XmiI GTMKAC 1 cut(s) 222
XmnI GAANNNNTTC 1 cut(s) 91
XspI CTAG 8 cut(s) 495, 602, 830, 918, 1275, 1343, 1652, 1670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.