RchiOBHm_Chr6g0309251

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001634
Physical Location & Seq
Reverse (-)
67058491 .. 67059832
1342 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27809

Sequence Viewer

Length: 1029 bp
ATGATTTTGACCAATACCGAAACATATGGTGAAGAGCTTGTTGCTGATGCATATTTCTTGCCTACAGCAGCAGTTGGCCAGAAAGCTGGTGACGCAATAAAGAGCTATATTGCATCCGATTCTAATCCAACAGCAACAATTTCTCTTGGAGAAACGGAGGTAGGCGTCCAACCTTCACCAGTAGTGGCTGCGTTTAGCTCTAGAGGACCGAATTCTGTCACTCCAGAAGTACTCAAACCAGACTTGATAGCACCAGGAATGAACATCCTAGCTGGGTGGACTGGCGCAGTTGGACCAACTGGACTTCCTGAAGACAAGAGGAGTGTGGTCTTCAACATCATTTCAGGTACATCCATGTCGTGTCCACACGTGAGTGGGCTCGCAGCCCTTGTCAAGGCGGCTCACCCAGACTGGAGCCCTGCGGCCATTAAGTCTGCCCTCATGACCACAGCCTATTCTAACTACAAAACTGGAGAAACAATCGAAGATGTTTCTACTGGAAGTGCTGCAACACCTTTCGATTATGGGGCTGGGCATGTGGATCCAGTGGCAGCTCTTGACCCTGGCCTTGTCTACGATCTTGCTGTTGAGGACTACCTAAGTTTCCTCTGTGCCTTGAATTATTCCGCTTCTAAGATCAAGCAAACCACTACGAAGGACTTCACCTGTGACTCAAGCAAGAAATACAGCCTCGGAAATTTGAATTACCCGTCCTTTTCTCTTGCTCTAGATACATCTTCAGGCCAAGGGGGTGGTACTGGTGCATCAACCACTTTAAAGTACACAAGGACTCTGACCAATGTGGGTGCCCCGGCAACATACAAGGTCTCAATGTCCTCACTGACCAGATCAGTGAAGATGTCCGTAGAACCAGAATCACTGAGTTTCAGTGATGCGTATGAGAAGAAGACATACACTGTGACTTTTGTTGCTAGCTCTTTGCCATCCGGTACAGCCAGCTTTGGTCGCTTGGAATGGTCTGATGGGAAACACATAGTAGGTAGTCCTATTGCTTACACCTGGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

342

Amino Acids

35.72

Weight (kDa)

5.05

Isoelectric Point (pI)

37.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 23 - 178 8.1e-22 Subtilase family
fn3_6 PF17766 233 - 338 7.4e-25 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 806
AccI GTMKAC 1 cut(s) 573
AciI CCGC 3 cut(s) 398, 422, 627
AclWI GGATC 2 cut(s) 536, 549
AcoI YGGCCR 2 cut(s) 76, 423
AcsI RAATTY 2 cut(s) 211, 697
AcuI CTGAAG 2 cut(s) 330, 723
AcvI CACGTG 1 cut(s) 370
AcyI GRCGYC 1 cut(s) 165
AfaI GTAC 5 cut(s) 231, 349, 757, 782, 952
AfiI CCNNNNNNNGG 1 cut(s) 394
AflIII ACRYGT 1 cut(s) 367
AgsI TTSAA 3 cut(s) 334, 619, 703
AjnI CCWGG 3 cut(s) 253, 562, 1019
AleI CACNNNNGTG 1 cut(s) 372
AluBI AGCT 8 cut(s) 37, 86, 105, 198, 272, 554, 936, 960
AluI AGCT 8 cut(s) 37, 86, 105, 198, 272, 554, 936, 960
Alw26I GTCTC 1 cut(s) 832
AlwI GGATC 2 cut(s) 536, 549
AoxI GGCC 4 cut(s) 76, 423, 565, 742
ApeKI GCWGC 5 cut(s) 68, 188, 383, 506, 551
ApoI RAATTY 2 cut(s) 211, 697
Asp700I GAANNNNTTC 1 cut(s) 659
AspLEI GCGC 1 cut(s) 287
AspS9I GGNCC 2 cut(s) 206, 293
AsuC2I CCSGG 1 cut(s) 812
AsuHPI GGTGA 5 cut(s) 41, 101, 168, 395, 655
AsuNHI GCTAGC 1 cut(s) 932
AvaII GGWCC 2 cut(s) 206, 293
BaeGI GKGCMC 1 cut(s) 811
BalI TGGCCA 1 cut(s) 78
BamHI GGATCC 1 cut(s) 541
BanI GGYRCC 1 cut(s) 806
BanII GRGCYC 2 cut(s) 381, 419
BarI GAAGNNNNNNTAC 2 cut(s) 896, 928
BbrPI CACGTG 1 cut(s) 370
BbsI GAAGAC 3 cut(s) 318, 322, 914
BbvI GCAGC 5 cut(s) 80, 175, 395, 493, 563
BccI CCATC 2 cut(s) 952, 977
BciT130I CCWGG 3 cut(s) 255, 564, 1021
BcnI CCSGG 1 cut(s) 812
BcoDI GTCTC 1 cut(s) 832
BfaI CTAG 4 cut(s) 201, 269, 728, 933
BfmI CTRYAG 1 cut(s) 63
BisI GCNGC 7 cut(s) 69, 189, 384, 399, 423, 507, 552
BlsI GCNGC 7 cut(s) 70, 190, 385, 400, 424, 508, 553
BmcAI AGTACT 1 cut(s) 231
Bme1390I CCNGG 4 cut(s) 255, 564, 812, 1021
Bme18I GGWCC 2 cut(s) 206, 293
BmgT120I GGNCC 2 cut(s) 206, 293
BmiI GGNNCC 3 cut(s) 416, 543, 808
BmrFI CCNGG 4 cut(s) 255, 564, 812, 1021
BmsI GCATC 4 cut(s) 37, 122, 773, 883
BmtI GCTAGC 1 cut(s) 936
BpiI GAAGAC 3 cut(s) 318, 322, 914
BpmI CTGGAG 3 cut(s) 207, 433, 492
BpuEI CTTGAG 1 cut(s) 658
BpuMI CCSGG 1 cut(s) 812
BsaAI YACGTR 1 cut(s) 370
BsaBI GATNNNNATC 1 cut(s) 123
BsaHI GRCGYC 1 cut(s) 165
BsaI GGTCTC 1 cut(s) 832
BsaJI CCNNGG 4 cut(s) 562, 691, 745, 810
BsaWI WCCGGW 1 cut(s) 947
Bsc4I CCNNNNNNNGG 1 cut(s) 394
Bse1I ACTGG 8 cut(s) 179, 286, 304, 416, 475, 502, 545, 763
Bse8I GATNNNNATC 1 cut(s) 123
BseBI CCWGG 3 cut(s) 255, 564, 1021
BseDI CCNNGG 4 cut(s) 562, 691, 745, 810
BseGI GGATG 4 cut(s) 113, 264, 350, 944
BseJI GATNNNNATC 1 cut(s) 123
BseLI CCNNNNNNNGG 1 cut(s) 394
BseMII CTCAG 1 cut(s) 872
BseNI ACTGG 8 cut(s) 179, 286, 304, 416, 475, 502, 545, 763
BseRI GAGGAG 1 cut(s) 334
BseSI GKGCMC 1 cut(s) 811
BseXI GCAGC 5 cut(s) 80, 175, 395, 493, 563
BseYI CCCAGC 2 cut(s) 272, 530
BshFI GGCC 4 cut(s) 78, 425, 567, 744
BshNI GGYRCC 1 cut(s) 806
BsiSI CCGG 2 cut(s) 812, 948
BslI CCNNNNNNNGG 1 cut(s) 394
BsmAI GTCTC 1 cut(s) 832
BsnI GGCC 4 cut(s) 78, 425, 567, 744
Bso31I GGTCTC 1 cut(s) 832
Bsp1286I GDGCHC 3 cut(s) 381, 419, 811
Bsp143I GATC 4 cut(s) 541, 577, 636, 848
BspACI CCGC 3 cut(s) 398, 422, 627
BspANI GGCC 4 cut(s) 78, 425, 567, 744
BspCNI CTCAG 1 cut(s) 873
BspHI TCATGA 1 cut(s) 441
BspLI GGNNCC 3 cut(s) 416, 543, 808
BspOI GCTAGC 1 cut(s) 936
BspPI GGATC 2 cut(s) 536, 549
BspQI GCTCTTC 1 cut(s) 27
BspT107I GGYRCC 1 cut(s) 806
BspTNI GGTCTC 1 cut(s) 832
BsrI ACTGG 8 cut(s) 179, 286, 304, 416, 475, 502, 545, 763
BssECI CCNNGG 4 cut(s) 562, 691, 745, 810
BssMI GATC 4 cut(s) 541, 577, 636, 848
BssNI GRCGYC 1 cut(s) 165
BssT1I CCWWGG 1 cut(s) 745
Bst2UI CCWGG 3 cut(s) 255, 564, 1021
Bst4CI ACNGT 1 cut(s) 919
Bst6I CTCTTC 1 cut(s) 27
BstACI GRCGYC 1 cut(s) 165
BstBAI YACGTR 1 cut(s) 370
BstC8I GCNNGC 3 cut(s) 381, 934, 958
BstDEI CTNAG 3 cut(s) 599, 633, 881
BstENI CCTNNNNNAGG 1 cut(s) 392
BstF5I GGATG 4 cut(s) 113, 264, 350, 944
BstHHI GCGC 1 cut(s) 287
BstKTI GATC 4 cut(s) 544, 580, 639, 851
BstMAI GTCTC 1 cut(s) 832
BstMBI GATC 4 cut(s) 541, 577, 636, 848
BstMWI GCNNNNNNNGC 2 cut(s) 92, 966
BstNI CCWGG 3 cut(s) 255, 564, 1021
BstNSI RCATGY 1 cut(s) 539
BstSCI CCNGG 4 cut(s) 253, 562, 810, 1019
BstSFI CTRYAG 1 cut(s) 63
BstSLI GKGCMC 1 cut(s) 811
BstV1I GCAGC 5 cut(s) 80, 175, 395, 493, 563
BstV2I GAAGAC 3 cut(s) 318, 322, 914
BstX2I RGATCY 1 cut(s) 541
BstXI CCANNNNNNTGG 2 cut(s) 86, 752
BstYI RGATCY 1 cut(s) 541
BsuRI GGCC 4 cut(s) 78, 425, 567, 744
BtsCI GGATG 4 cut(s) 113, 264, 350, 944
BtsIMutI CAGTG 6 cut(s) 552, 839, 858, 878, 895, 915
Cac8I GCNNGC 3 cut(s) 381, 934, 958
CciI TCATGA 1 cut(s) 441
CfoI GCGC 1 cut(s) 287
Cfr13I GGNCC 2 cut(s) 206, 293
CseI GACGC 2 cut(s) 101, 154
Csp6I GTAC 5 cut(s) 230, 348, 756, 781, 951
CviAII CATG 3 cut(s) 355, 442, 536
CviQI GTAC 5 cut(s) 230, 348, 756, 781, 951
DdeI CTNAG 3 cut(s) 599, 633, 881
DpnI GATC 4 cut(s) 543, 579, 638, 850
DpnII GATC 4 cut(s) 541, 577, 636, 848
DraI TTTAAA 1 cut(s) 777
EaeI YGGCCR 2 cut(s) 76, 423
Eam1104I CTCTTC 1 cut(s) 27
EarI CTCTTC 1 cut(s) 27
Eco130I CCWWGG 1 cut(s) 745
Eco24I GRGCYC 2 cut(s) 381, 419
Eco31I GGTCTC 1 cut(s) 832
Eco47I GGWCC 2 cut(s) 206, 293
Eco57I CTGAAG 2 cut(s) 330, 723
Eco72I CACGTG 1 cut(s) 370
EcoNI CCTNNNNNAGG 1 cut(s) 392
EcoRI GAATTC 1 cut(s) 211
EcoRII CCWGG 3 cut(s) 253, 562, 1019
EcoT14I CCWWGG 1 cut(s) 745
EcoT22I ATGCAT 1 cut(s) 52
EcoT38I GRGCYC 2 cut(s) 381, 419
ErhI CCWWGG 1 cut(s) 745
FaeI CATG 3 cut(s) 358, 445, 539
FatI CATG 3 cut(s) 354, 441, 535
FauNDI CATATG 1 cut(s) 25
FblI GTMKAC 1 cut(s) 573
Fnu4HI GCNGC 7 cut(s) 69, 189, 384, 399, 423, 507, 552
FokI GGATG 4 cut(s) 100, 251, 337, 931
FriOI GRGCYC 2 cut(s) 381, 419
Fsp4HI GCNGC 7 cut(s) 69, 189, 384, 399, 423, 507, 552
FspBI CTAG 4 cut(s) 201, 269, 728, 933
GlaI GCGC 1 cut(s) 286
GluI GCNGC 7 cut(s) 69, 189, 384, 399, 423, 507, 552
GsaI CCCAGC 2 cut(s) 276, 534
GsuI CTGGAG 3 cut(s) 207, 433, 492
HaeIII GGCC 4 cut(s) 78, 425, 567, 744
HapII CCGG 2 cut(s) 812, 948
HgaI GACGC 2 cut(s) 101, 154
HhaI GCGC 1 cut(s) 287
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 3 cut(s) 358, 445, 539
Hin6I GCGC 1 cut(s) 285
HinP1I GCGC 1 cut(s) 285
HinfI GANTC 4 cut(s) 119, 671, 790, 875
HpaII CCGG 2 cut(s) 812, 948
HphI GGTGA 5 cut(s) 41, 101, 168, 395, 655
Hpy166II GTNNAC 4 cut(s) 279, 365, 574, 783
Hpy188I TCNGA 4 cut(s) 118, 695, 795, 982
Hpy188III TCNNGA 6 cut(s) 201, 224, 308, 442, 557, 728
Hpy8I GTNNAC 4 cut(s) 279, 365, 574, 783
HpyAV CCTTC 2 cut(s) 183, 649
HpyCH4III ACNGT 1 cut(s) 919
HpyCH4IV ACGT 1 cut(s) 369
HpyCH4V TGCA 4 cut(s) 50, 113, 509, 764
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 966
HpyF3I CTNAG 3 cut(s) 599, 633, 881
HpySE526I ACGT 1 cut(s) 369
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 3 cut(s) 358, 445, 539
HspAI GCGC 1 cut(s) 285
Kzo9I GATC 4 cut(s) 541, 577, 636, 848
LguI GCTCTTC 1 cut(s) 27
LmnI GCTCC 1 cut(s) 414
Lsp1109I GCAGC 5 cut(s) 80, 175, 395, 493, 563
LweI GCATC 4 cut(s) 37, 122, 773, 883
MaeI CTAG 4 cut(s) 201, 269, 728, 933
MaeII ACGT 1 cut(s) 369
MaeIII GTNAC 4 cut(s) 89, 217, 668, 919
MalI GATC 4 cut(s) 543, 579, 638, 850
MboI GATC 4 cut(s) 541, 577, 636, 848
MboII GAAGA 8 cut(s) 44, 322, 323, 497, 729, 868, 916, 919
MflI RGATCY 1 cut(s) 541
MhlI GDGCHC 3 cut(s) 381, 419, 811
MlsI TGGCCA 1 cut(s) 78
MluCI AATT 5 cut(s) 138, 211, 619, 697, 703
MluNI TGGCCA 1 cut(s) 78
MlyI GAGTC 2 cut(s) 665, 784
MmeI TCCRAC 3 cut(s) 152, 193, 271
MnlI CCTC 8 cut(s) 151, 197, 312, 449, 583, 617, 701, 847
Mox20I TGGCCA 1 cut(s) 78
Mph1103I ATGCAT 1 cut(s) 52
MroXI GAANNNNTTC 1 cut(s) 659
MscI TGGCCA 1 cut(s) 78
MseI TTAA 2 cut(s) 429, 776
MslI CAYNNNNRTG 1 cut(s) 372
Msp20I TGGCCA 1 cut(s) 78
MspI CCGG 2 cut(s) 812, 948
MspR9I CCNGG 4 cut(s) 255, 564, 812, 1021
MvaI CCWGG 3 cut(s) 255, 564, 1021
MwoI GCNNNNNNNGC 2 cut(s) 92, 966
NciI CCSGG 1 cut(s) 812
NdeI CATATG 1 cut(s) 25
NdeII GATC 4 cut(s) 541, 577, 636, 848
NheI GCTAGC 1 cut(s) 932
NlaIII CATG 3 cut(s) 358, 445, 539
NlaIV GGNNCC 3 cut(s) 416, 543, 808
NmuCI GTSAC 4 cut(s) 89, 217, 668, 919
NsiI ATGCAT 1 cut(s) 52
NspI RCATGY 1 cut(s) 539
OliI CACNNNNGTG 1 cut(s) 372
PagI TCATGA 1 cut(s) 441
PciSI GCTCTTC 1 cut(s) 27
PdmI GAANNNNTTC 1 cut(s) 659
PfeI GAWTC 2 cut(s) 119, 875
PkrI GCNGC 7 cut(s) 70, 190, 385, 400, 424, 508, 553
PleI GAGTC 2 cut(s) 665, 784
PmaCI CACGTG 1 cut(s) 370
PmlI CACGTG 1 cut(s) 370
PpsI GAGTC 2 cut(s) 665, 784
Ppu21I YACGTR 1 cut(s) 370
Psp6I CCWGG 3 cut(s) 253, 562, 1019
PspCI CACGTG 1 cut(s) 370
PspFI CCCAGC 2 cut(s) 272, 530
PspGI CCWGG 3 cut(s) 253, 562, 1019
PspN4I GGNNCC 3 cut(s) 416, 543, 808
PspPI GGNCC 2 cut(s) 206, 293
PsuI RGATCY 1 cut(s) 541
RsaI GTAC 5 cut(s) 231, 349, 757, 782, 952
RsaNI GTAC 5 cut(s) 230, 348, 756, 781, 951
RseI CAYNNNNRTG 1 cut(s) 372
SapI GCTCTTC 1 cut(s) 27
SaqAI TTAA 2 cut(s) 429, 776
SatI GCNGC 7 cut(s) 69, 189, 384, 399, 423, 507, 552
Sau3AI GATC 4 cut(s) 541, 577, 636, 848
Sau96I GGNCC 2 cut(s) 206, 293
ScaI AGTACT 1 cut(s) 231
SchI GAGTC 2 cut(s) 665, 784
ScrFI CCNGG 4 cut(s) 255, 564, 812, 1021
SduI GDGCHC 3 cut(s) 381, 419, 811
SfaNI GCATC 4 cut(s) 37, 122, 773, 883
SfcI CTRYAG 1 cut(s) 63
SinI GGWCC 2 cut(s) 206, 293
SmiMI CAYNNNNRTG 1 cut(s) 372
SmlI CTYRAG 1 cut(s) 673
SmoI CTYRAG 1 cut(s) 673
Sse9I AATT 5 cut(s) 138, 211, 619, 697, 703
SsiI CCGC 3 cut(s) 398, 422, 627
SspMI CTAG 4 cut(s) 201, 269, 728, 933
StyD4I CCNGG 4 cut(s) 253, 562, 810, 1019
StyI CCWWGG 1 cut(s) 745
TaaI ACNGT 1 cut(s) 919
TaiI ACGT 1 cut(s) 372
TaqI TCGA 2 cut(s) 483, 519
TaqII GACCGA 1 cut(s) 223
TasI AATT 5 cut(s) 138, 211, 619, 697, 703
TatI WGTACW 2 cut(s) 229, 780
TauI GCSGC 2 cut(s) 401, 425
TfiI GAWTC 2 cut(s) 119, 875
Tru1I TTAA 2 cut(s) 429, 776
Tru9I TTAA 2 cut(s) 429, 776
TscAI CASTG 6 cut(s) 552, 846, 858, 885, 895, 922
TseFI GTSAC 4 cut(s) 89, 217, 668, 919
TseI GCWGC 5 cut(s) 68, 188, 383, 506, 551
Tsp45I GTSAC 4 cut(s) 89, 217, 668, 919
TspDTI ATGAA 1 cut(s) 275
TspGWI ACGGA 2 cut(s) 170, 853
TspRI CASTG 6 cut(s) 552, 846, 858, 885, 895, 922
VpaK11BI GGWCC 2 cut(s) 206, 293
XagI CCTNNNNNAGG 1 cut(s) 392
XapI RAATTY 2 cut(s) 211, 697
XbaI TCTAGA 2 cut(s) 200, 727
XceI RCATGY 1 cut(s) 539
XmiI GTMKAC 1 cut(s) 573
XmnI GAANNNNTTC 1 cut(s) 659
XspI CTAG 4 cut(s) 201, 269, 728, 933
ZrmI AGTACT 1 cut(s) 231
Zsp2I ATGCAT 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.