Rh6AG483900

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0001634
Physical Location & Seq
Reverse (-)
66331188 .. 66331820
633 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG483900.1

Sequence Viewer

Length: 633 bp
ATGGTCGCTGGGAAAATTGTGGTATGCGATCAGGGAGGAAGTAGGGTCAGAAAGAGCGTGGTGGTAAAAAAGGCTGGTGGGGTAGGGATGATTATGGCAAACATTGAAGGTTATGGAGAAGAACTTGTAGCCGACCCATATCTCTTGCCAACTGCATTGGTTGGCAAGAAAGCTGGTGATGCAATAAAAAGATATATTGCCTCGCATGCTAATCCGGAAGCCACATTGGTTTTCAGAAAGACTCAGTTGGGTATCAAGCCCTCACCGGTGGTGGCAGCATTCAGCTCTAGAGGACCCAATCCAGTCGCTACATCCCTACTCAAGCTAGACTTAATAGCACCAGGGGTTAATATTCTAGCTGGGTGGACTGGTGCAGATGGACCAACTGGACTACCTGAAGACAAAAAGACGGTTGGCTACAACATCATTTCAGGTACATCCATGTCATGCCCTCATGTGAGCGGGCTTGCCGCCCTTCTCAAGGCCGCTCACCCAAAATGGAGCCCTGCTGCCATTAAATCTGCTCTCATGACCACATCCTATACAAGAAGAAATGGAAAACCAATCAAAGATGTTGCTACTGGAAAGCCTGCAACACCATTTGATTATGGAGCGGGGCACGTGAACTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

210

Amino Acids

21.7

Weight (kDa)

9.73

Isoelectric Point (pI)

34.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PA PF02225 2 - 62 1.9e-08 PA domain
Peptidase_S8 PF00082 86 - 206 4.2e-20 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 3 cut(s) 462, 488, 614
AccIII TCCGGA 1 cut(s) 214
AciI CCGC 4 cut(s) 462, 471, 486, 614
AcuI CTGAAG 1 cut(s) 417
AcvI CACGTG 1 cut(s) 622
AfaI GTAC 1 cut(s) 436
AfiI CCNNNNNNNGG 1 cut(s) 481
AgeI ACCGGT 1 cut(s) 265
AgsI TTSAA 1 cut(s) 107
AjnI CCWGG 1 cut(s) 340
AluBI AGCT 4 cut(s) 173, 285, 325, 359
AluI AGCT 4 cut(s) 173, 285, 325, 359
Aor13HI TCCGGA 1 cut(s) 214
AoxI GGCC 1 cut(s) 483
ApeKI GCWGC 2 cut(s) 275, 509
AsiGI ACCGGT 1 cut(s) 265
AspS9I GGNCC 2 cut(s) 293, 380
AsuHPI GGTGA 3 cut(s) 188, 255, 482
AvaII GGWCC 2 cut(s) 293, 380
BaeGI GKGCMC 1 cut(s) 621
BanII GRGCYC 1 cut(s) 506
BbrPI CACGTG 1 cut(s) 622
BbsI GAAGAC 1 cut(s) 405
BbvI GCAGC 2 cut(s) 287, 496
BccI CCATC 1 cut(s) 371
BciT130I CCWGG 1 cut(s) 342
BfaI CTAG 3 cut(s) 288, 326, 356
BisI GCNGC 4 cut(s) 276, 471, 486, 510
BlsI GCNGC 4 cut(s) 277, 472, 487, 511
Bme1390I CCNGG 1 cut(s) 342
Bme18I GGWCC 2 cut(s) 293, 380
BmgT120I GGNCC 2 cut(s) 293, 380
BmiI GGNNCC 2 cut(s) 295, 503
BmrFI CCNGG 1 cut(s) 342
BmsI GCATC 1 cut(s) 169
BpiI GAAGAC 1 cut(s) 405
BpuEI CTTGAG 2 cut(s) 305, 464
BsaAI YACGTR 1 cut(s) 622
BsaJI CCNNGG 1 cut(s) 341
BsaWI WCCGGW 2 cut(s) 214, 265
Bsc4I CCNNNNNNNGG 1 cut(s) 481
Bse118I RCCGGY 1 cut(s) 265
Bse1I ACTGG 4 cut(s) 302, 373, 391, 586
BseAI TCCGGA 1 cut(s) 214
BseBI CCWGG 1 cut(s) 342
BseDI CCNNGG 1 cut(s) 341
BseGI GGATG 4 cut(s) 93, 311, 437, 536
BseLI CCNNNNNNNGG 1 cut(s) 481
BseMII CTCAG 1 cut(s) 257
BseNI ACTGG 4 cut(s) 302, 373, 391, 586
BseSI GKGCMC 1 cut(s) 621
BseXI GCAGC 2 cut(s) 287, 496
BseYI CCCAGC 2 cut(s) 8, 359
BsgI GTGCAG 1 cut(s) 393
BshFI GGCC 1 cut(s) 485
BshTI ACCGGT 1 cut(s) 265
BsiSI CCGG 2 cut(s) 215, 266
BslI CCNNNNNNNGG 1 cut(s) 481
BsmI GAATGC 1 cut(s) 278
BsnI GGCC 1 cut(s) 485
Bsp1286I GDGCHC 2 cut(s) 506, 621
Bsp13I TCCGGA 1 cut(s) 214
Bsp143I GATC 1 cut(s) 28
BspACI CCGC 4 cut(s) 462, 471, 486, 614
BspANI GGCC 1 cut(s) 485
BspCNI CTCAG 1 cut(s) 256
BspEI TCCGGA 1 cut(s) 214
BspHI TCATGA 1 cut(s) 528
BspLI GGNNCC 2 cut(s) 295, 503
BsrBI CCGCTC 3 cut(s) 462, 488, 614
BsrFI RCCGGY 1 cut(s) 265
BsrI ACTGG 4 cut(s) 302, 373, 391, 586
BssAI RCCGGY 1 cut(s) 265
BssECI CCNNGG 1 cut(s) 341
BssMI GATC 1 cut(s) 28
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 2 cut(s) 412, 629
BstBAI YACGTR 1 cut(s) 622
BstC8I GCNNGC 4 cut(s) 207, 464, 468, 591
BstDEI CTNAG 1 cut(s) 243
BstENI CCTNNNNNAGG 1 cut(s) 479
BstF5I GGATG 4 cut(s) 93, 311, 437, 536
BstKTI GATC 1 cut(s) 31
BstMBI GATC 1 cut(s) 28
BstMWI GCNNNNNNNGC 2 cut(s) 179, 206
BstNI CCWGG 1 cut(s) 342
BstNSI RCATGY 1 cut(s) 209
BstSCI CCNGG 1 cut(s) 340
BstSLI GKGCMC 1 cut(s) 621
BstV1I GCAGC 2 cut(s) 287, 496
BstV2I GAAGAC 1 cut(s) 405
BsuRI GGCC 1 cut(s) 485
BtsCI GGATG 4 cut(s) 93, 311, 437, 536
Cac8I GCNNGC 4 cut(s) 207, 464, 468, 591
CciI TCATGA 1 cut(s) 528
Cfr10I RCCGGY 1 cut(s) 265
Cfr13I GGNCC 2 cut(s) 293, 380
Csp6I GTAC 1 cut(s) 435
CspAI ACCGGT 1 cut(s) 265
CviAII CATG 5 cut(s) 206, 442, 447, 455, 529
CviQI GTAC 1 cut(s) 435
DdeI CTNAG 1 cut(s) 243
DpnI GATC 1 cut(s) 30
DpnII GATC 1 cut(s) 28
Eco24I GRGCYC 1 cut(s) 506
Eco47I GGWCC 2 cut(s) 293, 380
Eco57I CTGAAG 1 cut(s) 417
Eco72I CACGTG 1 cut(s) 622
EcoNI CCTNNNNNAGG 1 cut(s) 479
EcoO109I RGGNCCY 1 cut(s) 293
EcoRII CCWGG 1 cut(s) 340
EcoT38I GRGCYC 1 cut(s) 506
FaeI CATG 5 cut(s) 209, 445, 450, 458, 532
FalI AAGNNNNNCTT 2 cut(s) 314, 346
FatI CATG 5 cut(s) 205, 441, 446, 454, 528
FauI CCCGC 2 cut(s) 455, 607
Fnu4HI GCNGC 4 cut(s) 276, 471, 486, 510
FokI GGATG 4 cut(s) 100, 298, 424, 523
FriOI GRGCYC 1 cut(s) 506
Fsp4HI GCNGC 4 cut(s) 276, 471, 486, 510
FspBI CTAG 3 cut(s) 288, 326, 356
GluI GCNGC 4 cut(s) 276, 471, 486, 510
GsaI CCCAGC 2 cut(s) 12, 363
HaeIII GGCC 1 cut(s) 485
HapII CCGG 2 cut(s) 215, 266
Hin1II CATG 5 cut(s) 209, 445, 450, 458, 532
HinfI GANTC 1 cut(s) 241
HpaII CCGG 2 cut(s) 215, 266
HphI GGTGA 3 cut(s) 188, 255, 482
Hpy166II GTNNAC 2 cut(s) 366, 625
Hpy188I TCNGA 2 cut(s) 50, 236
Hpy188III TCNNGA 3 cut(s) 215, 288, 529
Hpy8I GTNNAC 2 cut(s) 366, 625
HpyAV CCTTC 2 cut(s) 101, 485
HpyCH4III ACNGT 2 cut(s) 412, 629
HpyCH4IV ACGT 1 cut(s) 621
HpyCH4V TGCA 4 cut(s) 155, 182, 374, 593
HpyF10VI GCNNNNNNNGC 2 cut(s) 179, 206
HpyF3I CTNAG 1 cut(s) 243
HpySE526I ACGT 1 cut(s) 621
Hsp92II CATG 5 cut(s) 209, 445, 450, 458, 532
Kpn2I TCCGGA 1 cut(s) 214
Kzo9I GATC 1 cut(s) 28
LmnI GCTCC 2 cut(s) 501, 611
Lsp1109I GCAGC 2 cut(s) 287, 496
LweI GCATC 1 cut(s) 169
MaeI CTAG 3 cut(s) 288, 326, 356
MaeII ACGT 1 cut(s) 621
MalI GATC 1 cut(s) 30
MbiI CCGCTC 3 cut(s) 462, 488, 614
MboI GATC 1 cut(s) 28
MboII GAAGA 3 cut(s) 131, 410, 561
MhlI GDGCHC 2 cut(s) 506, 621
MluCI AATT 1 cut(s) 15
MlyI GAGTC 1 cut(s) 235
MnlI CCTC 5 cut(s) 29, 211, 271, 284, 462
MroI TCCGGA 1 cut(s) 214
MseI TTAA 3 cut(s) 332, 348, 516
MspI CCGG 2 cut(s) 215, 266
MspR9I CCNGG 1 cut(s) 342
Mva1269I GAATGC 1 cut(s) 278
MvaI CCWGG 1 cut(s) 342
MwoI GCNNNNNNNGC 2 cut(s) 179, 206
NdeII GATC 1 cut(s) 28
NlaIII CATG 5 cut(s) 209, 445, 450, 458, 532
NlaIV GGNNCC 2 cut(s) 295, 503
NspI RCATGY 1 cut(s) 209
PaeI GCATGC 1 cut(s) 209
PagI TCATGA 1 cut(s) 528
PctI GAATGC 1 cut(s) 278
PinAI ACCGGT 1 cut(s) 265
PkrI GCNGC 4 cut(s) 277, 472, 487, 511
PleI GAGTC 1 cut(s) 235
PmaCI CACGTG 1 cut(s) 622
PmlI CACGTG 1 cut(s) 622
PpsI GAGTC 1 cut(s) 235
Ppu21I YACGTR 1 cut(s) 622
PpuMI RGGWCCY 1 cut(s) 293
Psp5II RGGWCCY 1 cut(s) 293
Psp6I CCWGG 1 cut(s) 340
PspCI CACGTG 1 cut(s) 622
PspFI CCCAGC 2 cut(s) 8, 359
PspGI CCWGG 1 cut(s) 340
PspN4I GGNNCC 2 cut(s) 295, 503
PspPI GGNCC 2 cut(s) 293, 380
PspPPI RGGWCCY 1 cut(s) 293
RsaI GTAC 1 cut(s) 436
RsaNI GTAC 1 cut(s) 435
SaqAI TTAA 3 cut(s) 332, 348, 516
SatI GCNGC 4 cut(s) 276, 471, 486, 510
Sau3AI GATC 1 cut(s) 28
Sau96I GGNCC 2 cut(s) 293, 380
SchI GAGTC 1 cut(s) 235
ScrFI CCNGG 1 cut(s) 342
SduI GDGCHC 2 cut(s) 506, 621
SetI ASST 8 cut(s) 112, 175, 287, 327, 361, 397, 436, 624
SfaNI GCATC 1 cut(s) 169
SgrAI CRCCGGYG 1 cut(s) 265
SinI GGWCC 2 cut(s) 293, 380
SmlI CTYRAG 2 cut(s) 320, 479
SmoI CTYRAG 2 cut(s) 320, 479
SphI GCATGC 1 cut(s) 209
Sse9I AATT 1 cut(s) 15
SsiI CCGC 4 cut(s) 462, 471, 486, 614
SspI AATATT 1 cut(s) 352
SspMI CTAG 3 cut(s) 288, 326, 356
StyD4I CCNGG 1 cut(s) 340
TaaI ACNGT 2 cut(s) 412, 629
TaiI ACGT 1 cut(s) 624
TasI AATT 1 cut(s) 15
TauI GCSGC 2 cut(s) 473, 488
Tru1I TTAA 3 cut(s) 332, 348, 516
Tru9I TTAA 3 cut(s) 332, 348, 516
TseI GCWGC 2 cut(s) 275, 509
VpaK11BI GGWCC 2 cut(s) 293, 380
XagI CCTNNNNNAGG 1 cut(s) 479
XbaI TCTAGA 1 cut(s) 287
XceI RCATGY 1 cut(s) 209
XspI CTAG 3 cut(s) 288, 326, 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.