Rroxscaffold_7G00159520

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001634
Physical Location & Seq
Forward (+)
2714564 .. 2716623
2060 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00159520.1

Sequence Viewer

Length: 2010 bp
ATGAAGAAGGCATCGAAATCCGTGCTTCTGCTAACGCTTATGGTTCTGGTTATCACTCACAACTGTGAGGTGGTAGCAGAAGAGAGTGAGCATGTGAAAAAGACGTACATCATTCACATGGACAAGTCCCAAATGCCCGCGAGTTTCGACGACGACCATGTCAACTGGTACGGCTCGTCGTTGAAAGCGGTCTCCGATTCAGCTGACATGCTTTACACGTACAAGACTGTCATCCATGGCTTCTCAACAAGGCTAACTGCCGAGGAAGCTGAGCAGCTCGAAAAGCAGCCAGGAATTCTCTCTGTCCTGCCGGAAAGGAGGTACGAGCTTCACACAACTAGGACTCCGGAGTTTCTCGGACTAGGCAAAAGCGAAGCTGTGTTACCGGCTTCGGATAAGATCGGTGAGGTGATCATCGGAGTAGTGGACACCGGCGTGTGGCCGGAGCTCAAAAGCTACGACGACACTGGTTTTGGACCAGTGCCGAGTGGCTGGAAAGGGTCGTGCCAGCAGGGAAAGACATTCAACTCTTCGAGATGCAACAAAAAGCTGATTGGTGCTGCATTTTTCTCAAACGGGTACGAAGCAGCGTTTGGACCTGTTGACGAAAACGTAGAATCAAAGTCACCGAGAGATGACGATGGTCATGGAACTCACACCTCAACAACTGCGGCGGGGTCTGTTGTTACAGGAGCTAGCTTCCTCGGTTATGCTCCAGGGACAGCTAGGGGTATGGCGCCGCAAGCTCGAGTGGCCACATACAAGGCTTGCTGGCTTGGAGGGTGTTTCGGTTCTGATATCATAGCTGCAATGGAGAAGGCGGTTGAGGATGGGAGACACGGTTGCCATTGGAGCTTTGCTGCAGCGGCGCAGGGTATTCTAGTATCGTGTTCGGCTGGAAATGGTGGACCGGAAGCGGGAAGCTTGTCCAATGTTGCGCCTTGGATAACTACTGTGGGCGCTGGAACATTGGACAGGGACTTCCCGGCTTATGTCAGCCTTGGAAATGCAAAGAAATTTAGAGGTATATCTCTATACCGGGGAACGGCCTTGTCTAGTGGATTGATTCCACTTGTTGATGCTCGCAATGCAAGCAGTTCGAGTGGTGATCTGTGCAGTCCCGACAGTCTGATTCCGGCAAAGGTTGCCGGGAAGATTGTGGTATGTGACCGAGGGGGAACTCCGAGGGTTCAAAAGAGTCTGGTGGTGAAAAAGGCTGGTGGTGTGGGAATGATTTTGACCAACACCGAAACATATGGTGAAGAGCTTGTTGCTGATGCATATTTCTTGCCTACAGCAGCAGTTGGCCAGAAAGCTGGTGACGCAATAAAGAGCTATATTGCATCCGATTCTAATCCAACAGCAACAATTTCTCTTGGAGAAACGGAGTTAGGCGTCCAACCTTCACCGGTGGTGGCTGCGTTTAGCTCTAGAGGACCGAATTTTATCACTCCACAAGTACTCAAACCAGACCTGATAGCACCAGGAATGAACATCCTAGCTGGGTGGACTGGCGCAGTTGGACCAACTGGACTTCCTGAAGACAAGAGGAGGGTGGGCTTCAACATCATTTCAGGTACATCCATGTCGTGTCCACACGTGAGTGGGCTCGCGGCCCTTGTCAAGGCGGCTCACCCAGACTGGAGCCCCGCGGCCATTAAGTCTGCCCTCATGACCACAGCCTATTCTAACTACAAAAGTACTGGAGAAACAATCGAAGATGTTTCTACTGGAAGTGCTGCAACACCTTTCGATTATGGGGCTGGGCATGTGGATCCAGTGGCAGCTCTTGACCCTGGCCTTGTCTACGATCTTGCTGTTGAAGACTACCTAAGTTTCCTCTGTGCCTTGAATTATTCCGCTTCTCAGATCAAGCAAACCACTAAGAAGGACTTCACCTGTGACTCAAGCAAGAAATACAGCCTCGGAGATTTGAATTACCCGTCCTTTTCTCTTGCTCTAGATACATCTTCAGGCCAAGGGGTGGTACTGGTGCATCAACCACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

669

Amino Acids

70.2

Weight (kDa)

5.74

Isoelectric Point (pI)

33.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 35 - 111 1.9e-19 Peptidase inhibitor I9
Peptidase_S8 PF00082 136 - 589 2.6e-38 Subtilase family
PA PF02225 357 - 443 6e-14 PA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 158
AccB1I GGYRCC 1 cut(s) 736
AccB7I CCANNNNNTGG 1 cut(s) 1984
AccI GTMKAC 1 cut(s) 1806
AccII CGCG 3 cut(s) 140, 1613, 1652
AccIII TCCGGA 1 cut(s) 346
AclWI GGATC 2 cut(s) 1769, 1782
AcoI YGGCCR 4 cut(s) 440, 753, 1306, 1653
AcsI RAATTY 3 cut(s) 294, 1016, 1441
AcuI CTGAAG 2 cut(s) 1560, 1956
AcvI CACGTG 1 cut(s) 1600
AcyI GRCGYC 2 cut(s) 737, 1395
AfaI GTAC 9 cut(s) 107, 170, 221, 323, 581, 1461, 1579, 1702, 1989
AfiI CCNNNNNNNGG 5 cut(s) 438, 917, 1045, 1624, 1984
AflIII ACRYGT 2 cut(s) 216, 1597
AgeI ACCGGT 1 cut(s) 1408
AgsI TTSAA 7 cut(s) 184, 526, 1193, 1564, 1823, 1852, 1936
AjnI CCWGG 4 cut(s) 289, 715, 1483, 1795
AjuI GAANNNNNNNTTGG 2 cut(s) 576, 608
AleI CACNNNNGTG 3 cut(s) 63, 434, 1602
Alw21I GWGCWC 1 cut(s) 450
Alw26I GTCTC 2 cut(s) 196, 829
AlwI GGATC 2 cut(s) 1769, 1782
Ama87I CYCGRG 1 cut(s) 747
Aor13HI TCCGGA 1 cut(s) 346
AoxI GGCC 8 cut(s) 440, 753, 1047, 1306, 1614, 1653, 1798, 1975
ApoI RAATTY 3 cut(s) 294, 1016, 1441
ArsI GACNNNNNNTTYG 2 cut(s) 455, 487
AsiGI ACCGGT 1 cut(s) 1408
Asp700I GAANNNNTTC 1 cut(s) 1892
AspLEI GCGC 5 cut(s) 739, 871, 940, 962, 1517
AspS9I GGNCC 6 cut(s) 476, 596, 908, 1436, 1523, 1615
AsuC2I CCSGG 3 cut(s) 986, 1040, 1150
AsuNHI GCTAGC 1 cut(s) 695
AvaI CYCGRG 1 cut(s) 747
AvaII GGWCC 5 cut(s) 476, 596, 908, 1436, 1523
BalI TGGCCA 2 cut(s) 755, 1308
BamHI GGATCC 1 cut(s) 1774
BanI GGYRCC 1 cut(s) 736
BanII GRGCYC 3 cut(s) 450, 1611, 1649
BarI GAAGNNNNNNTAC 2 cut(s) 366, 398
BbrPI CACGTG 1 cut(s) 1600
BbsI GAAGAC 2 cut(s) 1548, 1830
Bbv12I GWGCWC 1 cut(s) 450
BccI CCATC 2 cut(s) 635, 824
BceAI ACGGC 2 cut(s) 187, 1062
BcgI CGANNNNNNTGC 4 cut(s) 4, 38, 1080, 1114
BciT130I CCWGG 4 cut(s) 291, 717, 1485, 1797
BclI TGATCA 1 cut(s) 411
BcnI CCSGG 3 cut(s) 986, 1040, 1150
BcoDI GTCTC 2 cut(s) 196, 829
BfaI CTAG 9 cut(s) 339, 362, 696, 726, 881, 1056, 1431, 1499, 1961
BfmI CTRYAG 2 cut(s) 861, 1293
BfoI RGCGCY 2 cut(s) 740, 963
BlpI GCTNAGC 1 cut(s) 270
BmcAI AGTACT 2 cut(s) 1461, 1702
Bme1390I CCNGG 7 cut(s) 291, 717, 986, 1040, 1150, 1485, 1797
Bme18I GGWCC 5 cut(s) 476, 596, 908, 1436, 1523
BmeT110I CYCGRG 1 cut(s) 747
BmgT120I GGNCC 6 cut(s) 476, 596, 908, 1436, 1523, 1615
BmiI GGNNCC 3 cut(s) 738, 1646, 1776
BmrFI CCNGG 7 cut(s) 291, 717, 986, 1040, 1150, 1485, 1797
BmsI GCATC 6 cut(s) 20, 527, 1069, 1267, 1352, 2005
BmtI GCTAGC 1 cut(s) 699
BoxI GACNNNNGTC 1 cut(s) 642
BpiI GAAGAC 2 cut(s) 1548, 1830
BpmI CTGGAG 3 cut(s) 699, 1663, 1725
Bpu1102I GCTNAGC 1 cut(s) 270
BpuEI CTTGAG 1 cut(s) 1891
BpuMI CCSGG 3 cut(s) 986, 1040, 1150
BsaAI YACGTR 2 cut(s) 219, 1600
BsaBI GATNNNNATC 1 cut(s) 1353
BsaHI GRCGYC 2 cut(s) 737, 1395
BsaI GGTCTC 1 cut(s) 196
BsaWI WCCGGW 3 cut(s) 346, 910, 1408
BsaXI ACNNNNNCTCC 4 cut(s) 328, 358, 826, 856
Bsc4I CCNNNNNNNGG 5 cut(s) 438, 917, 1045, 1624, 1984
Bse118I RCCGGY 3 cut(s) 385, 431, 1408
Bse3DI GCAATG 2 cut(s) 816, 1093
Bse8I GATNNNNATC 1 cut(s) 1353
BseAI TCCGGA 1 cut(s) 346
BseBI CCWGG 4 cut(s) 291, 717, 1485, 1797
BseGI GGATG 5 cut(s) 231, 835, 1343, 1494, 1580
BseJI GATNNNNATC 1 cut(s) 1353
BseLI CCNNNNNNNGG 5 cut(s) 438, 917, 1045, 1624, 1984
BseMI GCAATG 2 cut(s) 816, 1093
BseMII CTCAG 2 cut(s) 261, 1880
BseRI GAGGAG 1 cut(s) 1564
BseYI CCCAGC 2 cut(s) 1502, 1763
BsgI GTGCAG 1 cut(s) 1135
Bsh1236I CGCG 3 cut(s) 140, 1613, 1652
BshFI GGCC 8 cut(s) 442, 755, 1049, 1308, 1616, 1655, 1800, 1977
BshNI GGYRCC 1 cut(s) 736
BshTI ACCGGT 1 cut(s) 1408
BsiHKAI GWGCWC 1 cut(s) 450
BsiHKCI CYCGRG 1 cut(s) 747
BslFI GGGAC 4 cut(s) 112, 733, 992, 1104
BslI CCNNNNNNNGG 5 cut(s) 438, 917, 1045, 1624, 1984
BsmAI GTCTC 2 cut(s) 196, 829
BsmFI GGGAC 4 cut(s) 112, 733, 992, 1104
BsnI GGCC 8 cut(s) 442, 755, 1049, 1308, 1616, 1655, 1800, 1977
Bso31I GGTCTC 1 cut(s) 196
BsoBI CYCGRG 1 cut(s) 747
Bsp1286I GDGCHC 3 cut(s) 450, 1611, 1649
Bsp13I TCCGGA 1 cut(s) 346
Bsp143I GATC 6 cut(s) 399, 411, 1108, 1774, 1810, 1869
Bsp1720I GCTNAGC 1 cut(s) 270
Bsp19I CCATGG 1 cut(s) 235
BspANI GGCC 8 cut(s) 442, 755, 1049, 1308, 1616, 1655, 1800, 1977
BspCNI CTCAG 2 cut(s) 262, 1879
BspEI TCCGGA 1 cut(s) 346
BspFNI CGCG 3 cut(s) 140, 1613, 1652
BspHI TCATGA 1 cut(s) 1671
BspLI GGNNCC 3 cut(s) 738, 1646, 1776
BspMAI CTGCAG 1 cut(s) 865
BspOI GCTAGC 1 cut(s) 699
BspPI GGATC 2 cut(s) 1769, 1782
BspQI GCTCTTC 1 cut(s) 1257
BspT107I GGYRCC 1 cut(s) 736
BspTNI GGTCTC 1 cut(s) 196
BsrDI GCAATG 2 cut(s) 816, 1093
BsrFI RCCGGY 3 cut(s) 385, 431, 1408
BssAI RCCGGY 3 cut(s) 385, 431, 1408
BssMI GATC 6 cut(s) 399, 411, 1108, 1774, 1810, 1869
BssNI GRCGYC 2 cut(s) 737, 1395
BssT1I CCWWGG 4 cut(s) 235, 941, 1000, 1978
Bst2UI CCWGG 4 cut(s) 291, 717, 1485, 1797
Bst4CI ACNGT 5 cut(s) 65, 229, 842, 955, 1127
Bst6I CTCTTC 3 cut(s) 75, 535, 1257
BstACI GRCGYC 2 cut(s) 737, 1395
BstAPI GCANNNNNTGC 1 cut(s) 1145
BstBAI YACGTR 2 cut(s) 219, 1600
BstC8I GCNNGC 9 cut(s) 138, 509, 697, 744, 769, 773, 1084, 1093, 1611
BstDEI CTNAG 4 cut(s) 270, 1832, 1866, 1884
BstDSI CCRYGG 2 cut(s) 235, 1650
BstENI CCTNNNNNAGG 1 cut(s) 1622
BstF5I GGATG 5 cut(s) 231, 835, 1343, 1494, 1580
BstFNI CGCG 3 cut(s) 140, 1613, 1652
BstH2I RGCGCY 2 cut(s) 740, 963
BstHHI GCGC 5 cut(s) 739, 871, 940, 962, 1517
BstKTI GATC 6 cut(s) 402, 414, 1111, 1777, 1813, 1872
BstMAI GTCTC 2 cut(s) 196, 829
BstMBI GATC 6 cut(s) 399, 411, 1108, 1774, 1810, 1869
BstNI CCWGG 4 cut(s) 291, 717, 1485, 1797
BstNSI RCATGY 3 cut(s) 95, 211, 1772
BstPAI GACNNNNGTC 1 cut(s) 642
BstSCI CCNGG 7 cut(s) 289, 715, 984, 1038, 1148, 1483, 1795
BstSFI CTRYAG 2 cut(s) 861, 1293
BstUI CGCG 3 cut(s) 140, 1613, 1652
BstV2I GAAGAC 2 cut(s) 1548, 1830
BstX2I RGATCY 1 cut(s) 1774
BstXI CCANNNNNNTGG 1 cut(s) 1316
BstYI RGATCY 1 cut(s) 1774
BsuRI GGCC 8 cut(s) 442, 755, 1049, 1308, 1616, 1655, 1800, 1977
BtgI CCRYGG 2 cut(s) 235, 1650
BtsCI GGATG 5 cut(s) 231, 835, 1343, 1494, 1580
BtsIMutI CAGTG 3 cut(s) 465, 486, 1785
Cac8I GCNNGC 9 cut(s) 138, 509, 697, 744, 769, 773, 1084, 1093, 1611
CciI TCATGA 1 cut(s) 1671
CfoI GCGC 5 cut(s) 739, 871, 940, 962, 1517
Cfr10I RCCGGY 3 cut(s) 385, 431, 1408
Cfr13I GGNCC 6 cut(s) 476, 596, 908, 1436, 1523, 1615
Cfr42I CCGCGG 1 cut(s) 1653
CseI GACGC 2 cut(s) 1331, 1384
Csp6I GTAC 9 cut(s) 106, 169, 220, 322, 580, 1460, 1578, 1701, 1988
CspAI ACCGGT 1 cut(s) 1408
CviAII CATG 9 cut(s) 92, 118, 158, 208, 236, 647, 1585, 1672, 1769
CviQI GTAC 9 cut(s) 106, 169, 220, 322, 580, 1460, 1578, 1701, 1988
DdeI CTNAG 4 cut(s) 270, 1832, 1866, 1884
DinI GGCGCC 1 cut(s) 738
DpnI GATC 6 cut(s) 401, 413, 1110, 1776, 1812, 1871
DpnII GATC 6 cut(s) 399, 411, 1108, 1774, 1810, 1869
DrdI GACNNNNNNGTC 1 cut(s) 158
DseDI GACNNNNNNGTC 1 cut(s) 158
EaeI YGGCCR 4 cut(s) 440, 753, 1306, 1653
Eam1104I CTCTTC 3 cut(s) 75, 535, 1257
EarI CTCTTC 3 cut(s) 75, 535, 1257
Ecl136II GAGCTC 1 cut(s) 448
Eco130I CCWWGG 4 cut(s) 235, 941, 1000, 1978
Eco24I GRGCYC 3 cut(s) 450, 1611, 1649
Eco31I GGTCTC 1 cut(s) 196
Eco32I GATATC 1 cut(s) 799
Eco47I GGWCC 5 cut(s) 476, 596, 908, 1436, 1523
Eco53kI GAGCTC 1 cut(s) 448
Eco57I CTGAAG 2 cut(s) 1560, 1956
Eco72I CACGTG 1 cut(s) 1600
Eco88I CYCGRG 1 cut(s) 747
EcoICRI GAGCTC 1 cut(s) 448
EcoNI CCTNNNNNAGG 1 cut(s) 1622
EcoRI GAATTC 1 cut(s) 294
EcoRII CCWGG 4 cut(s) 289, 715, 1483, 1795
EcoRV GATATC 1 cut(s) 799
EcoT14I CCWWGG 4 cut(s) 235, 941, 1000, 1978
EcoT22I ATGCAT 1 cut(s) 1282
EcoT38I GRGCYC 3 cut(s) 450, 1611, 1649
EgeI GGCGCC 1 cut(s) 738
EheI GGCGCC 1 cut(s) 738
ErhI CCWWGG 4 cut(s) 235, 941, 1000, 1978
FaeI CATG 9 cut(s) 95, 121, 161, 211, 239, 650, 1588, 1675, 1772
FalI AAGNNNNNCTT 2 cut(s) 1877, 1909
FaqI GGGAC 4 cut(s) 112, 733, 992, 1104
FatI CATG 9 cut(s) 91, 117, 157, 207, 235, 646, 1584, 1671, 1768
FauI CCCGC 4 cut(s) 145, 667, 910, 1657
FauNDI CATATG 1 cut(s) 1255
FbaI TGATCA 1 cut(s) 411
FblI GTMKAC 1 cut(s) 1806
FokI GGATG 5 cut(s) 218, 842, 1330, 1481, 1567
FriOI GRGCYC 3 cut(s) 450, 1611, 1649
FspBI CTAG 9 cut(s) 339, 362, 696, 726, 881, 1056, 1431, 1499, 1961
GlaI GCGC 5 cut(s) 738, 870, 939, 961, 1516
GsaI CCCAGC 2 cut(s) 1506, 1767
GsuI CTGGAG 3 cut(s) 699, 1663, 1725
HaeII RGCGCY 2 cut(s) 740, 963
HaeIII GGCC 8 cut(s) 442, 755, 1049, 1308, 1616, 1655, 1800, 1977
HgaI GACGC 2 cut(s) 1331, 1384
HhaI GCGC 5 cut(s) 739, 871, 940, 962, 1517
Hin1I GRCGYC 2 cut(s) 737, 1395
Hin1II CATG 9 cut(s) 95, 121, 161, 211, 239, 650, 1588, 1675, 1772
Hin6I GCGC 5 cut(s) 737, 869, 938, 960, 1515
HinP1I GCGC 5 cut(s) 737, 869, 938, 960, 1515
HincII GTYRAC 2 cut(s) 163, 604
HindII GTYRAC 2 cut(s) 163, 604
HindIII AAGCTT 1 cut(s) 922
HinfI GANTC 8 cut(s) 197, 343, 617, 1066, 1132, 1198, 1349, 1904
Hpy166II GTNNAC 7 cut(s) 163, 427, 604, 908, 1509, 1595, 1807
Hpy188III TCNNGA 8 cut(s) 347, 534, 1121, 1431, 1538, 1672, 1790, 1961
Hpy8I GTNNAC 7 cut(s) 163, 427, 604, 908, 1509, 1595, 1807
Hpy99I CGWCG 4 cut(s) 152, 155, 181, 464
HpyAV CCTTC 3 cut(s) 811, 1413, 1882
HpyCH4III ACNGT 5 cut(s) 65, 229, 842, 955, 1127
HpyCH4IV ACGT 4 cut(s) 104, 218, 612, 1599
HpyF3I CTNAG 4 cut(s) 270, 1832, 1866, 1884
HpySE526I ACGT 4 cut(s) 104, 218, 612, 1599
Hsp92I GRCGYC 2 cut(s) 737, 1395
Hsp92II CATG 9 cut(s) 95, 121, 161, 211, 239, 650, 1588, 1675, 1772
HspAI GCGC 5 cut(s) 737, 869, 938, 960, 1515
KasI GGCGCC 1 cut(s) 736
Kpn2I TCCGGA 1 cut(s) 346
Ksp22I TGATCA 1 cut(s) 411
KspI CCGCGG 1 cut(s) 1653
Kzo9I GATC 6 cut(s) 399, 411, 1108, 1774, 1810, 1869
LguI GCTCTTC 1 cut(s) 1257
LmnI GCTCC 5 cut(s) 445, 692, 718, 852, 1644
LweI GCATC 6 cut(s) 20, 527, 1069, 1267, 1352, 2005
MaeI CTAG 9 cut(s) 339, 362, 696, 726, 881, 1056, 1431, 1499, 1961
MaeII ACGT 4 cut(s) 104, 218, 612, 1599
MaeIII GTNAC 6 cut(s) 381, 624, 685, 1166, 1319, 1901
MalI GATC 6 cut(s) 401, 413, 1110, 1776, 1812, 1871
MboI GATC 6 cut(s) 399, 411, 1108, 1774, 1810, 1869
MboII GAAGA 9 cut(s) 16, 92, 522, 1165, 1274, 1553, 1730, 1835, 1962
MflI RGATCY 1 cut(s) 1774
MhlI GDGCHC 3 cut(s) 450, 1611, 1649
MlsI TGGCCA 2 cut(s) 755, 1308
MluCI AATT 6 cut(s) 294, 1016, 1368, 1441, 1852, 1936
MluNI TGGCCA 2 cut(s) 755, 1308
Mly113I GGCGCC 1 cut(s) 737
MlyI GAGTC 3 cut(s) 337, 1207, 1898
MmeI TCCRAC 3 cut(s) 1382, 1423, 1501
Mox20I TGGCCA 2 cut(s) 755, 1308
Mph1103I ATGCAT 1 cut(s) 1282
MroI TCCGGA 1 cut(s) 346
MroXI GAANNNNTTC 1 cut(s) 1892
MscI TGGCCA 2 cut(s) 755, 1308
MseI TTAA 2 cut(s) 1659, 2008
MslI CAYNNNNRTG 4 cut(s) 63, 116, 434, 1602
Msp20I TGGCCA 2 cut(s) 755, 1308
MspA1I CMGCKG 3 cut(s) 203, 866, 1652
MspR9I CCNGG 7 cut(s) 291, 717, 986, 1040, 1150, 1485, 1797
MvaI CCWGG 4 cut(s) 291, 717, 1485, 1797
MvnI CGCG 3 cut(s) 140, 1613, 1652
NarI GGCGCC 1 cut(s) 737
NciI CCSGG 3 cut(s) 986, 1040, 1150
NcoI CCATGG 1 cut(s) 235
NdeI CATATG 1 cut(s) 1255
NdeII GATC 6 cut(s) 399, 411, 1108, 1774, 1810, 1869
NheI GCTAGC 1 cut(s) 695
NlaIII CATG 9 cut(s) 95, 121, 161, 211, 239, 650, 1588, 1675, 1772
NlaIV GGNNCC 3 cut(s) 738, 1646, 1776
NmeAIII GCCGAG 2 cut(s) 286, 510
NmuCI GTSAC 4 cut(s) 624, 1166, 1319, 1901
NsiI ATGCAT 1 cut(s) 1282
NspI RCATGY 3 cut(s) 95, 211, 1772
OliI CACNNNNGTG 3 cut(s) 63, 434, 1602
PaeR7I CTCGAG 1 cut(s) 747
PagI TCATGA 1 cut(s) 1671
PciSI GCTCTTC 1 cut(s) 1257
PdmI GAANNNNTTC 1 cut(s) 1892
PfeI GAWTC 5 cut(s) 197, 617, 1066, 1132, 1349
PflFI GACNNNGTC 1 cut(s) 158
PflMI CCANNNNNTGG 1 cut(s) 1984
PinAI ACCGGT 1 cut(s) 1408
PleI GAGTC 3 cut(s) 337, 1206, 1898
PluTI GGCGCC 1 cut(s) 740
PmaCI CACGTG 1 cut(s) 1600
PmlI CACGTG 1 cut(s) 1600
PpsI GAGTC 3 cut(s) 337, 1206, 1898
Ppu21I YACGTR 2 cut(s) 219, 1600
PshAI GACNNNNGTC 1 cut(s) 642
Psp124BI GAGCTC 1 cut(s) 450
Psp6I CCWGG 4 cut(s) 289, 715, 1483, 1795
PspCI CACGTG 1 cut(s) 1600
PspFI CCCAGC 2 cut(s) 1502, 1763
PspGI CCWGG 4 cut(s) 289, 715, 1483, 1795
PspN4I GGNNCC 3 cut(s) 738, 1646, 1776
PspPI GGNCC 6 cut(s) 476, 596, 908, 1436, 1523, 1615
PspXI VCTCGAGB 1 cut(s) 747
PstI CTGCAG 1 cut(s) 865
PsuI RGATCY 1 cut(s) 1774
PsyI GACNNNGTC 1 cut(s) 158
PvuII CAGCTG 1 cut(s) 203
RsaI GTAC 9 cut(s) 107, 170, 221, 323, 581, 1461, 1579, 1702, 1989
RsaNI GTAC 9 cut(s) 106, 169, 220, 322, 580, 1460, 1578, 1701, 1988
RseI CAYNNNNRTG 4 cut(s) 63, 116, 434, 1602
SacI GAGCTC 1 cut(s) 450
SacII CCGCGG 1 cut(s) 1653
SapI GCTCTTC 1 cut(s) 1257
SaqAI TTAA 2 cut(s) 1659, 2008
Sau3AI GATC 6 cut(s) 399, 411, 1108, 1774, 1810, 1869
Sau96I GGNCC 6 cut(s) 476, 596, 908, 1436, 1523, 1615
ScaI AGTACT 2 cut(s) 1461, 1702
SchI GAGTC 3 cut(s) 337, 1207, 1898
ScrFI CCNGG 7 cut(s) 291, 717, 986, 1040, 1150, 1485, 1797
SduI GDGCHC 3 cut(s) 450, 1611, 1649
SfaNI GCATC 6 cut(s) 20, 527, 1069, 1267, 1352, 2005
SfcI CTRYAG 2 cut(s) 861, 1293
SfoI GGCGCC 1 cut(s) 738
Sfr274I CTCGAG 1 cut(s) 747
Sfr303I CCGCGG 1 cut(s) 1653
SgrAI CRCCGGYG 2 cut(s) 431, 1408
SgrBI CCGCGG 1 cut(s) 1653
SinI GGWCC 5 cut(s) 476, 596, 908, 1436, 1523
SlaI CTCGAG 1 cut(s) 747
SmiMI CAYNNNNRTG 4 cut(s) 63, 116, 434, 1602
SmlI CTYRAG 2 cut(s) 747, 1906
SmoI CTYRAG 2 cut(s) 747, 1906
Sse9I AATT 6 cut(s) 294, 1016, 1368, 1441, 1852, 1936
SspDI GGCGCC 1 cut(s) 736
SspMI CTAG 9 cut(s) 339, 362, 696, 726, 881, 1056, 1431, 1499, 1961
SstI GAGCTC 1 cut(s) 450
StyD4I CCNGG 7 cut(s) 289, 715, 984, 1038, 1148, 1483, 1795
StyI CCWWGG 4 cut(s) 235, 941, 1000, 1978
TaaI ACNGT 5 cut(s) 65, 229, 842, 955, 1127
TaiI ACGT 4 cut(s) 107, 221, 615, 1602
TaqI TCGA 8 cut(s) 14, 147, 279, 533, 748, 1100, 1716, 1752
TaqII GACCGA 2 cut(s) 1185, 1453
TasI AATT 6 cut(s) 294, 1016, 1368, 1441, 1852, 1936
TatI WGTACW 2 cut(s) 1459, 1700
TauI GCSGC 6 cut(s) 674, 742, 869, 1616, 1631, 1655
TfiI GAWTC 5 cut(s) 197, 617, 1066, 1132, 1349
Tru1I TTAA 2 cut(s) 1659, 2008
Tru9I TTAA 2 cut(s) 1659, 2008
TscAI CASTG 3 cut(s) 472, 486, 1785
TseFI GTSAC 4 cut(s) 624, 1166, 1319, 1901
Tsp45I GTSAC 4 cut(s) 624, 1166, 1319, 1901
TspDTI ATGAA 2 cut(s) 17, 1505
TspGWI ACGGA 2 cut(s) 10, 1400
TspRI CASTG 3 cut(s) 472, 486, 1785
Tth111I GACNNNGTC 1 cut(s) 158
Van91I CCANNNNNTGG 1 cut(s) 1984
VpaK11BI GGWCC 5 cut(s) 476, 596, 908, 1436, 1523
XagI CCTNNNNNAGG 1 cut(s) 1622
XapI RAATTY 3 cut(s) 294, 1016, 1441
XbaI TCTAGA 2 cut(s) 1430, 1960
XceI RCATGY 3 cut(s) 95, 211, 1772
XhoI CTCGAG 1 cut(s) 747
XmiI GTMKAC 1 cut(s) 1806
XmnI GAANNNNTTC 1 cut(s) 1892
XspI CTAG 9 cut(s) 339, 362, 696, 726, 881, 1056, 1431, 1499, 1961
ZrmI AGTACT 2 cut(s) 1461, 1702
Zsp2I ATGCAT 1 cut(s) 1282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.