subtilisin-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 4 cut(s) | 404, 425, 824, 1226 |
| AatII | GACGTC | 1 cut(s) | 402 |
| AccB1I | GGYRCC | 3 cut(s) | 548, 1433, 1565 |
| AccBSI | CCGCTC | 2 cut(s) | 1656, 2016 |
| AccI | GTMKAC | 2 cut(s) | 1593, 1854 |
| AclI | AACGTT | 1 cut(s) | 831 |
| AcsI | RAATTY | 1 cut(s) | 2005 |
| AcvI | CACGTG | 3 cut(s) | 495, 1651, 1819 |
| AcyI | GRCGYC | 5 cut(s) | 248, 399, 456, 1115, 1566 |
| AfaI | GTAC | 7 cut(s) | 161, 314, 557, 701, 962, 2048, 2087 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 283, 382, 666, 720, 1429, 1831, 1843 |
| AflIII | ACRYGT | 3 cut(s) | 165, 494, 1816 |
| AgeI | ACCGGT | 1 cut(s) | 1580 |
| AgsI | TTSAA | 5 cut(s) | 310, 517, 1615, 1900, 2044 |
| AjiI | CACGTC | 2 cut(s) | 1632, 1771 |
| AjnI | CCWGG | 4 cut(s) | 551, 611, 737, 1534 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 270, 302 |
| AleI | CACNNNNGTG | 1 cut(s) | 2066 |
| Alw21I | GWGCWC | 4 cut(s) | 98, 147, 321, 935 |
| Alw26I | GTCTC | 2 cut(s) | 257, 618 |
| Ama87I | CYCGRG | 7 cut(s) | 281, 302, 338, 1236, 1482, 2050, 2234 |
| ApaI | GGGCCC | 2 cut(s) | 1684, 2075 |
| ApeKI | GCWGC | 4 cut(s) | 241, 1339, 1669, 2220 |
| ApoI | RAATTY | 1 cut(s) | 2005 |
| AsiGI | ACCGGT | 1 cut(s) | 1580 |
| AspLEI | GCGC | 9 cut(s) | 706, 718, 959, 1004, 1534, 1568, 1605, 1669, 1720 |
| AsuHPI | GGTGA | 6 cut(s) | 670, 1912, 2023, 2053, 2131, 2197 |
| AvaI | CYCGRG | 7 cut(s) | 281, 302, 338, 1236, 1482, 2050, 2234 |
| AvaII | GGWCC | 9 cut(s) | 333, 467, 615, 669, 950, 1240, 1285, 1560, 1822 |
| BaeGI | GKGCMC | 5 cut(s) | 494, 1010, 1436, 1684, 2075 |
| BalI | TGGCCA | 4 cut(s) | 5, 433, 728, 800 |
| BamHI | GGATCC | 2 cut(s) | 710, 1399 |
| BanI | GGYRCC | 3 cut(s) | 548, 1433, 1565 |
| BanII | GRGCYC | 4 cut(s) | 321, 1684, 1700, 2075 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 1135, 1167, 2162, 2194 |
| BauI | CACGAG | 2 cut(s) | 508, 928 |
| BbrPI | CACGTG | 3 cut(s) | 495, 1651, 1819 |
| BbsI | GAAGAC | 1 cut(s) | 2019 |
| Bbv12I | GWGCWC | 4 cut(s) | 98, 147, 321, 935 |
| BbvCI | CCTCAGC | 1 cut(s) | 1037 |
| BbvI | GCAGC | 4 cut(s) | 253, 1326, 1656, 2232 |
| BceAI | ACGGC | 7 cut(s) | 244, 649, 1324, 1365, 1384, 1428, 1755 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1423, 1457, 1790, 1824 |
| BciT130I | CCWGG | 4 cut(s) | 553, 613, 739, 1536 |
| BcoDI | GTCTC | 2 cut(s) | 257, 618 |
| BfaI | CTAG | 3 cut(s) | 12, 81, 954 |
| BfoI | RGCGCY | 2 cut(s) | 1569, 1721 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1739 |
| BmcAI | AGTACT | 1 cut(s) | 2048 |
| Bme18I | GGWCC | 9 cut(s) | 333, 467, 615, 669, 950, 1240, 1285, 1560, 1822 |
| BmeT110I | CYCGRG | 7 cut(s) | 281, 302, 338, 1236, 1482, 2050, 2234 |
| BmgBI | CACGTC | 2 cut(s) | 1632, 1771 |
| BmsI | GCATC | 1 cut(s) | 189 |
| BpiI | GAAGAC | 1 cut(s) | 2019 |
| BpmI | CTGGAG | 1 cut(s) | 444 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1037 |
| BpuEI | CTTGAG | 2 cut(s) | 254, 1658 |
| BsaAI | YACGTR | 3 cut(s) | 495, 1651, 1819 |
| BsaBI | GATNNNNATC | 3 cut(s) | 923, 1404, 2276 |
| BsaHI | GRCGYC | 5 cut(s) | 248, 399, 456, 1115, 1566 |
| BsaWI | WCCGGW | 4 cut(s) | 680, 1459, 1580, 1778 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 218, 248, 599, 629, 1607, 1637, 2126, 2156 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 283, 382, 666, 720, 1429, 1831, 1843 |
| Bse118I | RCCGGY | 5 cut(s) | 130, 1130, 1342, 1580, 1922 |
| Bse1I | ACTGG | 5 cut(s) | 161, 393, 427, 1017, 1140 |
| Bse8I | GATNNNNATC | 3 cut(s) | 923, 1404, 2276 |
| BseBI | CCWGG | 4 cut(s) | 553, 613, 739, 1536 |
| BseGI | GGATG | 4 cut(s) | 222, 729, 1787, 2113 |
| BseJI | GATNNNNATC | 3 cut(s) | 923, 1404, 2276 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 283, 382, 666, 720, 1429, 1831, 1843 |
| BseMII | CTCAG | 2 cut(s) | 1051, 1077 |
| BseNI | ACTGG | 5 cut(s) | 161, 393, 427, 1017, 1140 |
| BseRI | GAGGAG | 3 cut(s) | 269, 511, 1328 |
| BseSI | GKGCMC | 5 cut(s) | 494, 1010, 1436, 1684, 2075 |
| BseX3I | CGGCCG | 4 cut(s) | 192, 936, 1413, 2076 |
| BseXI | GCAGC | 4 cut(s) | 253, 1326, 1656, 2232 |
| BseYI | CCCAGC | 1 cut(s) | 665 |
| Bsh1285I | CGRYCG | 7 cut(s) | 195, 939, 1222, 1416, 1593, 1715, 2079 |
| BshNI | GGYRCC | 3 cut(s) | 548, 1433, 1565 |
| BshTI | ACCGGT | 1 cut(s) | 1580 |
| BsiEI | CGRYCG | 7 cut(s) | 195, 939, 1222, 1416, 1593, 1715, 2079 |
| BsiHKAI | GWGCWC | 4 cut(s) | 98, 147, 321, 935 |
| BsiHKCI | CYCGRG | 7 cut(s) | 281, 302, 338, 1236, 1482, 2050, 2234 |
| BslFI | GGGAC | 2 cut(s) | 601, 890 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 283, 382, 666, 720, 1429, 1831, 1843 |
| BsmAI | GTCTC | 2 cut(s) | 257, 618 |
| BsmBI | CGTCTC | 2 cut(s) | 257, 618 |
| BsmFI | GGGAC | 2 cut(s) | 601, 890 |
| BsoBI | CYCGRG | 7 cut(s) | 281, 302, 338, 1236, 1482, 2050, 2234 |
| Bsp120I | GGGCCC | 2 cut(s) | 1680, 2071 |
| Bsp19I | CCATGG | 1 cut(s) | 2103 |
| Bsp68I | TCGCGA | 1 cut(s) | 1020 |
| BspCNI | CTCAG | 2 cut(s) | 1050, 1076 |
| BspQI | GCTCTTC | 1 cut(s) | 377 |
| BspT107I | GGYRCC | 3 cut(s) | 548, 1433, 1565 |
| BsrBI | CCGCTC | 2 cut(s) | 1656, 2016 |
| BsrFI | RCCGGY | 5 cut(s) | 130, 1130, 1342, 1580, 1922 |
| BsrI | ACTGG | 5 cut(s) | 161, 393, 427, 1017, 1140 |
| BssAI | RCCGGY | 5 cut(s) | 130, 1130, 1342, 1580, 1922 |
| BssNI | GRCGYC | 5 cut(s) | 248, 399, 456, 1115, 1566 |
| BssSI | CACGAG | 2 cut(s) | 508, 928 |
| BssT1I | CCWWGG | 3 cut(s) | 603, 1437, 2103 |
| Bst2BI | CACGAG | 2 cut(s) | 508, 928 |
| Bst2UI | CCWGG | 4 cut(s) | 553, 613, 739, 1536 |
| Bst4CI | ACNGT | 9 cut(s) | 965, 997, 1052, 1223, 1255, 1808, 1867, 2038, 2185 |
| Bst6I | CTCTTC | 2 cut(s) | 377, 2168 |
| BstACI | GRCGYC | 5 cut(s) | 248, 399, 456, 1115, 1566 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1762 |
| BstBAI | YACGTR | 3 cut(s) | 495, 1651, 1819 |
| BstC8I | GCNNGC | 8 cut(s) | 132, 481, 688, 735, 1006, 1344, 1646, 1948 |
| BstDEI | CTNAG | 3 cut(s) | 1037, 1063, 1549 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1427 |
| BstF5I | GGATG | 4 cut(s) | 222, 729, 1787, 2113 |
| BstH2I | RGCGCY | 2 cut(s) | 1569, 1721 |
| BstHHI | GCGC | 9 cut(s) | 706, 718, 959, 1004, 1534, 1568, 1605, 1669, 1720 |
| BstMAI | GTCTC | 2 cut(s) | 257, 618 |
| BstMCI | CGRYCG | 7 cut(s) | 195, 939, 1222, 1416, 1593, 1715, 2079 |
| BstNI | CCWGG | 4 cut(s) | 553, 613, 739, 1536 |
| BstSLI | GKGCMC | 5 cut(s) | 494, 1010, 1436, 1684, 2075 |
| BstV1I | GCAGC | 4 cut(s) | 253, 1326, 1656, 2232 |
| BstV2I | GAAGAC | 1 cut(s) | 2019 |
| BstX2I | RGATCY | 5 cut(s) | 710, 893, 918, 1399, 1978 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1105, 1585 |
| BstYI | RGATCY | 5 cut(s) | 710, 893, 918, 1399, 1978 |
| BstZI | CGGCCG | 4 cut(s) | 192, 936, 1413, 2076 |
| BtgZI | GCGATG | 3 cut(s) | 902, 920, 1386 |
| BtrI | CACGTC | 2 cut(s) | 1632, 1771 |
| BtsCI | GGATG | 4 cut(s) | 222, 729, 1787, 2113 |
| BtsIMutI | CAGTG | 5 cut(s) | 154, 400, 420, 1010, 2034 |
| BtuMI | TCGCGA | 1 cut(s) | 1020 |
| Cac8I | GCNNGC | 8 cut(s) | 132, 481, 688, 735, 1006, 1344, 1646, 1948 |
| CfoI | GCGC | 9 cut(s) | 706, 718, 959, 1004, 1534, 1568, 1605, 1669, 1720 |
| Cfr10I | RCCGGY | 5 cut(s) | 130, 1130, 1342, 1580, 1922 |
| Cfr42I | CCGCGG | 4 cut(s) | 60, 663, 1084, 2160 |
| Cfr9I | CCCGGG | 2 cut(s) | 281, 1236 |
| CseI | GACGC | 8 cut(s) | 86, 156, 173, 256, 274, 464, 1123, 1337 |
| Csp6I | GTAC | 7 cut(s) | 160, 313, 556, 700, 961, 2047, 2086 |
| CspAI | ACCGGT | 1 cut(s) | 1580 |
| CviAII | CATG | 5 cut(s) | 7, 112, 1168, 1282, 2104 |
| CviQI | GTAC | 7 cut(s) | 160, 313, 556, 700, 961, 2047, 2086 |
| DdeI | CTNAG | 3 cut(s) | 1037, 1063, 1549 |
| DinI | GGCGCC | 1 cut(s) | 1567 |
| DrdI | GACNNNNNNGTC | 4 cut(s) | 404, 425, 824, 1226 |
| DseDI | GACNNNNNNGTC | 4 cut(s) | 404, 425, 824, 1226 |
| EagI | CGGCCG | 4 cut(s) | 192, 936, 1413, 2076 |
| Eam1104I | CTCTTC | 2 cut(s) | 377, 2168 |
| EarI | CTCTTC | 2 cut(s) | 377, 2168 |
| EciI | GGCGGA | 2 cut(s) | 89, 870 |
| Ecl136II | GAGCTC | 1 cut(s) | 319 |
| EclXI | CGGCCG | 4 cut(s) | 192, 936, 1413, 2076 |
| Eco130I | CCWWGG | 3 cut(s) | 603, 1437, 2103 |
| Eco24I | GRGCYC | 4 cut(s) | 321, 1684, 1700, 2075 |
| Eco47I | GGWCC | 9 cut(s) | 333, 467, 615, 669, 950, 1240, 1285, 1560, 1822 |
| Eco52I | CGGCCG | 4 cut(s) | 192, 936, 1413, 2076 |
| Eco53kI | GAGCTC | 1 cut(s) | 319 |
| Eco72I | CACGTG | 3 cut(s) | 495, 1651, 1819 |
| Eco88I | CYCGRG | 7 cut(s) | 281, 302, 338, 1236, 1482, 2050, 2234 |
| EcoICRI | GAGCTC | 1 cut(s) | 319 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1427 |
| EcoO109I | RGGNCCY | 4 cut(s) | 587, 615, 950, 1680 |
| EcoRII | CCWGG | 4 cut(s) | 551, 611, 737, 1534 |
| EcoT14I | CCWWGG | 3 cut(s) | 603, 1437, 2103 |
| EcoT38I | GRGCYC | 4 cut(s) | 321, 1684, 1700, 2075 |
| EgeI | GGCGCC | 1 cut(s) | 1567 |
| EheI | GGCGCC | 1 cut(s) | 1567 |
| ErhI | CCWWGG | 3 cut(s) | 603, 1437, 2103 |
| Esp3I | CGTCTC | 2 cut(s) | 257, 618 |
| FaeI | CATG | 5 cut(s) | 10, 115, 1171, 1285, 2107 |
| FaiI | YATR | 7 cut(s) | 8, 113, 803, 842, 1169, 1283, 2105 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 905, 937 |
| FaqI | GGGAC | 2 cut(s) | 601, 890 |
| FatI | CATG | 5 cut(s) | 6, 111, 1167, 1281, 2103 |
| FauI | CCCGC | 5 cut(s) | 764, 940, 997, 1653, 1708 |
| FblI | GTMKAC | 2 cut(s) | 1593, 1854 |
| FokI | GGATG | 4 cut(s) | 209, 736, 1774, 2120 |
| FriOI | GRGCYC | 4 cut(s) | 321, 1684, 1700, 2075 |
| FspBI | CTAG | 3 cut(s) | 12, 81, 954 |
| GlaI | GCGC | 9 cut(s) | 705, 717, 958, 1003, 1533, 1567, 1604, 1668, 1719 |
| GsaI | CCCAGC | 1 cut(s) | 669 |
| GsuI | CTGGAG | 1 cut(s) | 444 |
| HaeII | RGCGCY | 2 cut(s) | 1569, 1721 |
| HgaI | GACGC | 8 cut(s) | 86, 156, 173, 256, 274, 464, 1123, 1337 |
| HhaI | GCGC | 9 cut(s) | 706, 718, 959, 1004, 1534, 1568, 1605, 1669, 1720 |
| Hin1I | GRCGYC | 5 cut(s) | 248, 399, 456, 1115, 1566 |
| Hin1II | CATG | 5 cut(s) | 10, 115, 1171, 1285, 2107 |
| Hin6I | GCGC | 9 cut(s) | 704, 716, 957, 1002, 1532, 1566, 1603, 1667, 1718 |
| HinP1I | GCGC | 9 cut(s) | 704, 716, 957, 1002, 1532, 1566, 1603, 1667, 1718 |
| HincII | GTYRAC | 4 cut(s) | 829, 1231, 1543, 1594 |
| HindII | GTYRAC | 4 cut(s) | 829, 1231, 1543, 1594 |
| HphI | GGTGA | 6 cut(s) | 670, 1912, 2023, 2053, 2131, 2197 |
| Hpy166II | GTNNAC | 8 cut(s) | 109, 829, 1231, 1543, 1594, 1822, 1855, 2034 |
| Hpy188III | TCNNGA | 8 cut(s) | 19, 302, 793, 922, 1019, 1243, 1482, 2208 |
| Hpy8I | GTNNAC | 8 cut(s) | 109, 829, 1231, 1543, 1594, 1822, 1855, 2034 |
| HpyAV | CCTTC | 6 cut(s) | 481, 907, 1192, 1464, 1807, 2001 |
| HpyCH4III | ACNGT | 9 cut(s) | 965, 997, 1052, 1223, 1255, 1808, 1867, 2038, 2185 |
| HpyCH4V | TGCA | 2 cut(s) | 1167, 1765 |
| HpyF3I | CTNAG | 3 cut(s) | 1037, 1063, 1549 |
| Hsp92I | GRCGYC | 5 cut(s) | 248, 399, 456, 1115, 1566 |
| Hsp92II | CATG | 5 cut(s) | 10, 115, 1171, 1285, 2107 |
| HspAI | GCGC | 9 cut(s) | 704, 716, 957, 1002, 1532, 1566, 1603, 1667, 1718 |
| KasI | GGCGCC | 1 cut(s) | 1565 |
| KroI | GCCGGC | 2 cut(s) | 130, 1342 |
| KroNI | GCCGGC | 2 cut(s) | 132, 1344 |
| KspI | CCGCGG | 4 cut(s) | 60, 663, 1084, 2160 |
| LguI | GCTCTTC | 1 cut(s) | 377 |
| LmnI | GCTCC | 9 cut(s) | 93, 324, 683, 788, 985, 1315, 1383, 1695, 2134 |
| Lsp1109I | GCAGC | 4 cut(s) | 253, 1326, 1656, 2232 |
| LweI | GCATC | 1 cut(s) | 189 |
| MaeI | CTAG | 3 cut(s) | 12, 81, 954 |
| MaeIII | GTNAC | 8 cut(s) | 395, 676, 1133, 1142, 1964, 2177, 2185, 2197 |
| MbiI | CCGCTC | 2 cut(s) | 1656, 2016 |
| MboII | GAAGA | 6 cut(s) | 290, 364, 2019, 2134, 2182, 2185 |
| MflI | RGATCY | 5 cut(s) | 710, 893, 918, 1399, 1978 |
| MlsI | TGGCCA | 4 cut(s) | 5, 433, 728, 800 |
| MluCI | AATT | 2 cut(s) | 1087, 2005 |
| MluI | ACGCGT | 1 cut(s) | 165 |
| MluNI | TGGCCA | 4 cut(s) | 5, 433, 728, 800 |
| Mly113I | GGCGCC | 1 cut(s) | 1566 |
| MlyI | GAGTC | 9 cut(s) | 345, 435, 456, 617, 1236, 1589, 1919, 2050, 2150 |
| Mox20I | TGGCCA | 4 cut(s) | 5, 433, 728, 800 |
| MroNI | GCCGGC | 2 cut(s) | 130, 1342 |
| MscI | TGGCCA | 4 cut(s) | 5, 433, 728, 800 |
| MslI | CAYNNNNRTG | 3 cut(s) | 794, 1634, 2066 |
| Msp20I | TGGCCA | 4 cut(s) | 5, 433, 728, 800 |
| MspA1I | CMGCKG | 5 cut(s) | 59, 244, 662, 1083, 2159 |
| MvaI | CCWGG | 4 cut(s) | 553, 613, 739, 1536 |
| NaeI | GCCGGC | 2 cut(s) | 132, 1344 |
| NarI | GGCGCC | 1 cut(s) | 1566 |
| NcoI | CCATGG | 1 cut(s) | 2103 |
| NgoMIV | GCCGGC | 2 cut(s) | 130, 1342 |
| NlaIII | CATG | 5 cut(s) | 10, 115, 1171, 1285, 2107 |
| NmeAIII | GCCGAG | 8 cut(s) | 170, 220, 286, 503, 828, 881, 914, 956 |
| NmuCI | GTSAC | 3 cut(s) | 395, 676, 2185 |
| NruI | TCGCGA | 1 cut(s) | 1020 |
| OliI | CACNNNNGTG | 1 cut(s) | 2066 |
| PaeR7I | CTCGAG | 2 cut(s) | 2050, 2234 |
| PciSI | GCTCTTC | 1 cut(s) | 377 |
| PcsI | WCGNNNNNNNCGW | 6 cut(s) | 300, 822, 1374, 1824, 2027, 2090 |
| PdiI | GCCGGC | 2 cut(s) | 132, 1344 |
| PfeI | GAWTC | 4 cut(s) | 1159, 1275, 1405, 1446 |
| PflFI | GACNNNGTC | 2 cut(s) | 824, 1526 |
| PfoI | TCCNGGA | 1 cut(s) | 1154 |
| PinAI | ACCGGT | 1 cut(s) | 1580 |
| Ple19I | CGATCG | 1 cut(s) | 1715 |
| PleI | GAGTC | 9 cut(s) | 345, 434, 456, 616, 1235, 1589, 1918, 2050, 2149 |
| PluTI | GGCGCC | 1 cut(s) | 1569 |
| PmaCI | CACGTG | 3 cut(s) | 495, 1651, 1819 |
| PmlI | CACGTG | 3 cut(s) | 495, 1651, 1819 |
| PpsI | GAGTC | 9 cut(s) | 345, 434, 456, 616, 1235, 1589, 1918, 2050, 2149 |
| Ppu21I | YACGTR | 3 cut(s) | 495, 1651, 1819 |
| PpuMI | RGGWCCY | 2 cut(s) | 615, 950 |
| Psp124BI | GAGCTC | 1 cut(s) | 321 |
| Psp1406I | AACGTT | 1 cut(s) | 831 |
| Psp5II | RGGWCCY | 2 cut(s) | 615, 950 |
| Psp6I | CCWGG | 4 cut(s) | 551, 611, 737, 1534 |
| PspCI | CACGTG | 3 cut(s) | 495, 1651, 1819 |
| PspFI | CCCAGC | 1 cut(s) | 665 |
| PspGI | CCWGG | 4 cut(s) | 551, 611, 737, 1534 |
| PspOMI | GGGCCC | 2 cut(s) | 1680, 2071 |
| PspPPI | RGGWCCY | 2 cut(s) | 615, 950 |
| PspXI | VCTCGAGB | 2 cut(s) | 2050, 2234 |
| PsuI | RGATCY | 5 cut(s) | 710, 893, 918, 1399, 1978 |
| PsyI | GACNNNGTC | 2 cut(s) | 824, 1526 |
| PvuI | CGATCG | 1 cut(s) | 1715 |
| PvuII | CAGCTG | 1 cut(s) | 244 |
| RruI | TCGCGA | 1 cut(s) | 1020 |
| RsaI | GTAC | 7 cut(s) | 161, 314, 557, 701, 962, 2048, 2087 |
| RsaNI | GTAC | 7 cut(s) | 160, 313, 556, 700, 961, 2047, 2086 |
| RseI | CAYNNNNRTG | 3 cut(s) | 794, 1634, 2066 |
| SacI | GAGCTC | 1 cut(s) | 321 |
| SacII | CCGCGG | 4 cut(s) | 60, 663, 1084, 2160 |
| SalI | GTCGAC | 1 cut(s) | 1592 |
| SapI | GCTCTTC | 1 cut(s) | 377 |
| ScaI | AGTACT | 1 cut(s) | 2048 |
| SchI | GAGTC | 9 cut(s) | 345, 435, 456, 617, 1236, 1589, 1919, 2050, 2150 |
| SfaNI | GCATC | 1 cut(s) | 189 |
| SfoI | GGCGCC | 1 cut(s) | 1567 |
| Sfr274I | CTCGAG | 2 cut(s) | 2050, 2234 |
| Sfr303I | CCGCGG | 4 cut(s) | 60, 663, 1084, 2160 |
| SgrBI | CCGCGG | 4 cut(s) | 60, 663, 1084, 2160 |
| SinI | GGWCC | 9 cut(s) | 333, 467, 615, 669, 950, 1240, 1285, 1560, 1822 |
| SlaI | CTCGAG | 2 cut(s) | 2050, 2234 |
| SmaI | CCCGGG | 2 cut(s) | 283, 1238 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 794, 1634, 2066 |
| SmlI | CTYRAG | 4 cut(s) | 269, 1673, 2050, 2234 |
| SmoI | CTYRAG | 4 cut(s) | 269, 1673, 2050, 2234 |
| Sse9I | AATT | 2 cut(s) | 1087, 2005 |
| SspDI | GGCGCC | 1 cut(s) | 1565 |
| SspMI | CTAG | 3 cut(s) | 12, 81, 954 |
| SstI | GAGCTC | 1 cut(s) | 321 |
| StyI | CCWWGG | 3 cut(s) | 603, 1437, 2103 |
| TaaI | ACNGT | 9 cut(s) | 965, 997, 1052, 1223, 1255, 1808, 1867, 2038, 2185 |
| TasI | AATT | 2 cut(s) | 1087, 2005 |
| TatI | WGTACW | 1 cut(s) | 2046 |
| TfiI | GAWTC | 4 cut(s) | 1159, 1275, 1405, 1446 |
| TscAI | CASTG | 5 cut(s) | 161, 400, 427, 1017, 2041 |
| TseFI | GTSAC | 3 cut(s) | 395, 676, 2185 |
| TseI | GCWGC | 4 cut(s) | 241, 1339, 1669, 2220 |
| Tsp45I | GTSAC | 3 cut(s) | 395, 676, 2185 |
| TspGWI | ACGGA | 3 cut(s) | 654, 661, 972 |
| TspMI | CCCGGG | 2 cut(s) | 281, 1236 |
| TspRI | CASTG | 5 cut(s) | 161, 400, 427, 1017, 2041 |
| Tth111I | GACNNNGTC | 2 cut(s) | 824, 1526 |
| VpaK11BI | GGWCC | 9 cut(s) | 333, 467, 615, 669, 950, 1240, 1285, 1560, 1822 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1427 |
| XapI | RAATTY | 1 cut(s) | 2005 |
| XhoI | CTCGAG | 2 cut(s) | 2050, 2234 |
| XmaI | CCCGGG | 2 cut(s) | 281, 1236 |
| XmiI | GTMKAC | 2 cut(s) | 1593, 1854 |
| XspI | CTAG | 3 cut(s) | 12, 81, 954 |
| ZraI | GACGTC | 1 cut(s) | 400 |
| ZrmI | AGTACT | 1 cut(s) | 2048 |