Rh6CG497800

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0001634
Physical Location & Seq
Reverse (-)
66254245 .. 66257396
3152 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG497800.1

Sequence Viewer

Length: 1296 bp
ATGGTGTCAACATTTTATCTTTGTCTGTCTTTTCCGGGAGCACAATACGAGGATTTCTCCACAGACATTGTTGCTATTGGAGCTTTTTCTGCCATGGCCAAGGGAGTATTTGTGTCATGTTCAGCCGGAAATGATGGGCCAATTTCCTATACCTCATCCAACAATGCACCTTGGATAACCACTGTGGGTGCTGGAACACTAGACCGGGACTTCCCAGCTTATGTTAGTTTTGGAAATGGAAAGAAATACAGAGGTATAGCACTTGACCGCGGTATAGTATCATTATCTAGTGGATTACATCCCCTTGTATATGCTTCTAATGCAAGTAACTCCACAAGTGGTGATCAATGCTCTCCTAACAGTTTGATTCCTCAGATGGTTGCTGGGAAAATTGTGGTATGCGATCGGGGAGGAAGTAGGGTCAGAAAGAGTGTGGTGGTAAAAAAGGCTGGTGGGGTAGGGATGATTATGGCAAACATTGAAGGTTATGGAGAAGAACTTGTAGCCGACCCATATCTCTTGCCAACTGCATTGGTTGGCAAGAAAGCTGGTGATGCAATAAGAAGATATATTGCCTCGCATGCTAATCCGGAAGCCACATTGGTTTTCAGAAAGACTCAGTTGGGTATCGAACCCTCACCAGTGGTGGCAGCATTCAGCTCTAGAGGACCCAATCCAGTCGCTACATCACTACTCAAGCCAGACTTGATAGCACCAGGAGTTAATATTCTAGCTGGGTGGACTGGTGCAGATGGACCAACTGGACTACCTGAAGACAAAAAGAGGGTTAGCTACAACATCATTTCAGGTACATCCATGTCATGCCCTCATGTGAGCGGGCTTGCCGCCCTTCTCAAGGCCGCTCACCCAAAATGGAGCCCTGCTGCCATTAAATCTGCTCTCATGACCACATCCTATACAAGAAGAAATGGAAAACCAATCAAAGATGTTGCTACTGGAAAGCCTGCAACACCATTTGATTATGGAGCTGGGCACGTGAACCCAGTTGGAGCCCTTGACCCTGGCCTTATATATGATCTAGCTGTGGAGGACTACCTAGGCTTCCTCTGTGCCATGAATTACACGACGAAAGATATCAAGAAACTCACTCACAGAGACTCCACCTGTGACTCAAGAAAGAAATACAGAGTTCGAGATTTCAACTACCCATCTCTTGCTGCTACCAGTCGGGGGTGGATCTGTCAATTACACAAGGACTCTAACCAATGTGGGTACCCCAGCAAGATACAAGGTCCGAGTGACTACAAAGTCCCCATTAGTGAAGATCTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

431

Amino Acids

45.69

Weight (kDa)

9.02

Isoelectric Point (pI)

32.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 20 - 331 7.8e-26 Subtilase family
PA PF02225 101 - 187 6.5e-11 PA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1268
Acc65I GGTACC 1 cut(s) 1233
AccB1I GGYRCC 1 cut(s) 1233
AccBSI CCGCTC 2 cut(s) 837, 863
AccII CGCG 1 cut(s) 270
AccIII TCCGGA 1 cut(s) 589
AciI CCGC 5 cut(s) 268, 270, 837, 846, 861
AclWI GGATC 1 cut(s) 1205
AcoI YGGCCR 1 cut(s) 96
AcuI CTGAAG 1 cut(s) 792
AcvI CACGTG 1 cut(s) 997
AfaI GTAC 2 cut(s) 811, 1235
AfiI CCNNNNNNNGG 2 cut(s) 856, 1191
AgsI TTSAA 2 cut(s) 482, 1162
AjnI CCWGG 2 cut(s) 715, 1021
AluBI AGCT 8 cut(s) 83, 218, 548, 660, 734, 792, 989, 1043
AluI AGCT 8 cut(s) 83, 218, 548, 660, 734, 792, 989, 1043
Alw21I GWGCWC 1 cut(s) 43
Alw26I GTCTC 1 cut(s) 1110
AlwI GGATC 1 cut(s) 1205
Aor13HI TCCGGA 1 cut(s) 589
AoxI GGCC 4 cut(s) 96, 137, 858, 1024
ApeKI GCWGC 3 cut(s) 650, 884, 1178
Asp718I GGTACC 1 cut(s) 1233
AspA2I CCTAGG 1 cut(s) 1057
AspS9I GGNCC 4 cut(s) 137, 668, 755, 1253
AsuC2I CCSGG 2 cut(s) 36, 206
AsuHPI GGTGA 4 cut(s) 353, 563, 630, 857
AvaII GGWCC 3 cut(s) 668, 755, 1253
AvrII CCTAGG 1 cut(s) 1057
BaeGI GKGCMC 1 cut(s) 996
BalI TGGCCA 1 cut(s) 98
BanI GGYRCC 1 cut(s) 1233
BanII GRGCYC 2 cut(s) 881, 1015
BbrPI CACGTG 1 cut(s) 997
BbsI GAAGAC 1 cut(s) 780
Bbv12I GWGCWC 1 cut(s) 43
BbvI GCAGC 3 cut(s) 662, 871, 1165
BccI CCATC 4 cut(s) 128, 370, 746, 1177
BciT130I CCWGG 2 cut(s) 717, 1023
BclI TGATCA 1 cut(s) 343
BcnI CCSGG 2 cut(s) 36, 206
BcoDI GTCTC 1 cut(s) 1110
BfaI CTAG 6 cut(s) 200, 288, 663, 731, 1040, 1058
BglII AGATCT 1 cut(s) 1285
BisI GCNGC 5 cut(s) 651, 846, 861, 885, 1179
BlnI CCTAGG 1 cut(s) 1057
BlsI GCNGC 5 cut(s) 652, 847, 862, 886, 1180
Bme1390I CCNGG 4 cut(s) 36, 206, 717, 1023
Bme18I GGWCC 3 cut(s) 668, 755, 1253
BmgT120I GGNCC 4 cut(s) 137, 668, 755, 1253
BmiI GGNNCC 4 cut(s) 670, 878, 1012, 1235
BmrFI CCNGG 4 cut(s) 36, 206, 717, 1023
BmrI ACTGGG 1 cut(s) 998
BmsI GCATC 1 cut(s) 544
BmuI ACTGGG 1 cut(s) 998
BpiI GAAGAC 1 cut(s) 780
BplI GAGNNNNNCTC 2 cut(s) 41, 73
BpuEI CTTGAG 3 cut(s) 680, 839, 1117
BpuMI CCSGG 2 cut(s) 36, 206
BsaAI YACGTR 1 cut(s) 997
BsaJI CCNNGG 6 cut(s) 93, 99, 170, 268, 1021, 1057
BsaWI WCCGGW 1 cut(s) 589
BsaXI ACNNNNNCTCC 4 cut(s) 96, 126, 1103, 1133
Bsc4I CCNNNNNNNGG 2 cut(s) 856, 1191
Bse1I ACTGG 7 cut(s) 641, 677, 748, 766, 961, 1004, 1185
BseAI TCCGGA 1 cut(s) 589
BseBI CCWGG 2 cut(s) 717, 1023
BseDI CCNNGG 6 cut(s) 93, 99, 170, 268, 1021, 1057
BseGI GGATG 5 cut(s) 155, 298, 468, 812, 911
BseLI CCNNNNNNNGG 2 cut(s) 856, 1191
BseMII CTCAG 2 cut(s) 386, 632
BseNI ACTGG 7 cut(s) 641, 677, 748, 766, 961, 1004, 1185
BseSI GKGCMC 1 cut(s) 996
BseXI GCAGC 3 cut(s) 662, 871, 1165
BseYI CCCAGC 5 cut(s) 214, 383, 734, 989, 1238
BsgI GTGCAG 1 cut(s) 768
Bsh1236I CGCG 1 cut(s) 270
Bsh1285I CGRYCG 1 cut(s) 406
BshFI GGCC 4 cut(s) 98, 139, 860, 1026
BshNI GGYRCC 1 cut(s) 1233
BsiEI CGRYCG 1 cut(s) 406
BsiHKAI GWGCWC 1 cut(s) 43
BsiSI CCGG 4 cut(s) 35, 126, 205, 590
BslFI GGGAC 2 cut(s) 221, 1256
BslI CCNNNNNNNGG 2 cut(s) 856, 1191
BsmAI GTCTC 1 cut(s) 1110
BsmFI GGGAC 2 cut(s) 221, 1256
BsmI GAATGC 1 cut(s) 653
BsnI GGCC 4 cut(s) 98, 139, 860, 1026
Bsp1286I GDGCHC 4 cut(s) 43, 881, 996, 1015
Bsp13I TCCGGA 1 cut(s) 589
Bsp143I GATC 5 cut(s) 343, 403, 1036, 1197, 1285
Bsp19I CCATGG 1 cut(s) 93
BspACI CCGC 5 cut(s) 268, 270, 837, 846, 861
BspANI GGCC 4 cut(s) 98, 139, 860, 1026
BspCNI CTCAG 2 cut(s) 385, 631
BspEI TCCGGA 1 cut(s) 589
BspFNI CGCG 1 cut(s) 270
BspHI TCATGA 1 cut(s) 903
BspLI GGNNCC 4 cut(s) 670, 878, 1012, 1235
BspPI GGATC 1 cut(s) 1205
BspT107I GGYRCC 1 cut(s) 1233
BsrBI CCGCTC 2 cut(s) 837, 863
BsrI ACTGG 7 cut(s) 641, 677, 748, 766, 961, 1004, 1185
BssECI CCNNGG 6 cut(s) 93, 99, 170, 268, 1021, 1057
BssMI GATC 5 cut(s) 343, 403, 1036, 1197, 1285
BssT1I CCWWGG 4 cut(s) 93, 99, 170, 1057
Bst2UI CCWGG 2 cut(s) 717, 1023
Bst4CI ACNGT 2 cut(s) 184, 362
BstBAI YACGTR 1 cut(s) 997
BstC8I GCNNGC 4 cut(s) 582, 839, 843, 966
BstDEI CTNAG 2 cut(s) 372, 618
BstDSI CCRYGG 2 cut(s) 93, 268
BstENI CCTNNNNNAGG 1 cut(s) 854
BstF5I GGATG 5 cut(s) 155, 298, 468, 812, 911
BstFNI CGCG 1 cut(s) 270
BstKTI GATC 5 cut(s) 346, 406, 1039, 1200, 1288
BstMAI GTCTC 1 cut(s) 1110
BstMBI GATC 5 cut(s) 343, 403, 1036, 1197, 1285
BstMCI CGRYCG 1 cut(s) 406
BstMWI GCNNNNNNNGC 5 cut(s) 80, 89, 320, 554, 581
BstNI CCWGG 2 cut(s) 717, 1023
BstNSI RCATGY 1 cut(s) 584
BstSCI CCNGG 4 cut(s) 34, 204, 715, 1021
BstSLI GKGCMC 1 cut(s) 996
BstUI CGCG 1 cut(s) 270
BstV1I GCAGC 3 cut(s) 662, 871, 1165
BstV2I GAAGAC 1 cut(s) 780
BstX2I RGATCY 2 cut(s) 1197, 1285
BstYI RGATCY 2 cut(s) 1197, 1285
BsuRI GGCC 4 cut(s) 98, 139, 860, 1026
BtgI CCRYGG 2 cut(s) 93, 268
BtsCI GGATG 5 cut(s) 155, 298, 468, 812, 911
BtsIMutI CAGTG 2 cut(s) 180, 648
Cac8I GCNNGC 4 cut(s) 582, 839, 843, 966
CciI TCATGA 1 cut(s) 903
Cfr13I GGNCC 4 cut(s) 137, 668, 755, 1253
Cfr42I CCGCGG 1 cut(s) 271
Csp6I GTAC 2 cut(s) 810, 1234
CspCI CAANNNNNGTGG 2 cut(s) 49, 84
CviAII CATG 8 cut(s) 94, 117, 581, 817, 822, 830, 904, 1075
CviQI GTAC 2 cut(s) 810, 1234
DdeI CTNAG 2 cut(s) 372, 618
DpnI GATC 5 cut(s) 345, 405, 1038, 1199, 1287
DpnII GATC 5 cut(s) 343, 403, 1036, 1197, 1285
DrdI GACNNNNNNGTC 1 cut(s) 1268
DseDI GACNNNNNNGTC 1 cut(s) 1268
EaeI YGGCCR 1 cut(s) 96
Eco130I CCWWGG 4 cut(s) 93, 99, 170, 1057
Eco24I GRGCYC 2 cut(s) 881, 1015
Eco32I GATATC 1 cut(s) 1096
Eco47I GGWCC 3 cut(s) 668, 755, 1253
Eco57I CTGAAG 1 cut(s) 792
Eco72I CACGTG 1 cut(s) 997
EcoNI CCTNNNNNAGG 1 cut(s) 854
EcoO109I RGGNCCY 1 cut(s) 668
EcoRII CCWGG 2 cut(s) 715, 1021
EcoRV GATATC 1 cut(s) 1096
EcoT14I CCWWGG 4 cut(s) 93, 99, 170, 1057
EcoT38I GRGCYC 2 cut(s) 881, 1015
ErhI CCWWGG 4 cut(s) 93, 99, 170, 1057
FaeI CATG 8 cut(s) 97, 120, 584, 820, 825, 833, 907, 1078
FalI AAGNNNNNCTT 2 cut(s) 689, 721
FaqI GGGAC 2 cut(s) 221, 1256
FatI CATG 8 cut(s) 93, 116, 580, 816, 821, 829, 903, 1074
FauI CCCGC 1 cut(s) 830
FbaI TGATCA 1 cut(s) 343
Fnu4HI GCNGC 5 cut(s) 651, 846, 861, 885, 1179
FokI GGATG 5 cut(s) 142, 285, 475, 799, 898
FriOI GRGCYC 2 cut(s) 881, 1015
Fsp4HI GCNGC 5 cut(s) 651, 846, 861, 885, 1179
FspBI CTAG 6 cut(s) 200, 288, 663, 731, 1040, 1058
GluI GCNGC 5 cut(s) 651, 846, 861, 885, 1179
GsaI CCCAGC 5 cut(s) 218, 387, 738, 993, 1242
HaeIII GGCC 4 cut(s) 98, 139, 860, 1026
HapII CCGG 4 cut(s) 35, 126, 205, 590
Hin1II CATG 8 cut(s) 97, 120, 584, 820, 825, 833, 907, 1078
HincII GTYRAC 1 cut(s) 9
HindII GTYRAC 1 cut(s) 9
HinfI GANTC 5 cut(s) 367, 616, 1118, 1130, 1217
HpaII CCGG 4 cut(s) 35, 126, 205, 590
HphI GGTGA 4 cut(s) 353, 563, 630, 857
Hpy166II GTNNAC 3 cut(s) 9, 741, 1000
Hpy188I TCNGA 4 cut(s) 375, 425, 611, 1257
Hpy188III TCNNGA 7 cut(s) 590, 663, 904, 1099, 1134, 1154, 1289
Hpy8I GTNNAC 3 cut(s) 9, 741, 1000
Hpy99I CGWCG 1 cut(s) 1090
HpyAV CCTTC 2 cut(s) 476, 860
HpyCH4III ACNGT 2 cut(s) 184, 362
HpyCH4IV ACGT 1 cut(s) 996
HpyCH4V TGCA 6 cut(s) 167, 323, 530, 557, 749, 968
HpyF10VI GCNNNNNNNGC 5 cut(s) 80, 89, 320, 554, 581
HpyF3I CTNAG 2 cut(s) 372, 618
HpySE526I ACGT 1 cut(s) 996
Hsp92II CATG 8 cut(s) 97, 120, 584, 820, 825, 833, 907, 1078
Kpn2I TCCGGA 1 cut(s) 589
KpnI GGTACC 1 cut(s) 1237
Ksp22I TGATCA 1 cut(s) 343
KspI CCGCGG 1 cut(s) 271
Kzo9I GATC 5 cut(s) 343, 403, 1036, 1197, 1285
LmnI GCTCC 5 cut(s) 38, 80, 876, 986, 1010
Lsp1109I GCAGC 3 cut(s) 662, 871, 1165
LweI GCATC 1 cut(s) 544
MaeI CTAG 6 cut(s) 200, 288, 663, 731, 1040, 1058
MaeII ACGT 1 cut(s) 996
MaeIII GTNAC 3 cut(s) 326, 1127, 1259
MalI GATC 5 cut(s) 345, 405, 1038, 1199, 1287
MbiI CCGCTC 2 cut(s) 837, 863
MboI GATC 5 cut(s) 343, 403, 1036, 1197, 1285
MboII GAAGA 5 cut(s) 506, 576, 785, 936, 1295
MflI RGATCY 2 cut(s) 1197, 1285
MhlI GDGCHC 4 cut(s) 43, 881, 996, 1015
MlsI TGGCCA 1 cut(s) 98
MluCI AATT 4 cut(s) 141, 390, 1078, 1205
MluNI TGGCCA 1 cut(s) 98
MlyI GAGTC 4 cut(s) 610, 1112, 1124, 1211
MmeI TCCRAC 2 cut(s) 183, 988
Mox20I TGGCCA 1 cut(s) 98
MroI TCCGGA 1 cut(s) 589
MscI TGGCCA 1 cut(s) 98
MseI TTAA 3 cut(s) 723, 891, 1294
Msp20I TGGCCA 1 cut(s) 98
MspA1I CMGCKG 1 cut(s) 270
MspI CCGG 4 cut(s) 35, 126, 205, 590
MspR9I CCNGG 4 cut(s) 36, 206, 717, 1023
Mva1269I GAATGC 1 cut(s) 653
MvaI CCWGG 2 cut(s) 717, 1023
MvnI CGCG 1 cut(s) 270
MwoI GCNNNNNNNGC 5 cut(s) 80, 89, 320, 554, 581
NciI CCSGG 2 cut(s) 36, 206
NcoI CCATGG 1 cut(s) 93
NdeII GATC 5 cut(s) 343, 403, 1036, 1197, 1285
NlaIII CATG 8 cut(s) 97, 120, 584, 820, 825, 833, 907, 1078
NlaIV GGNNCC 4 cut(s) 670, 878, 1012, 1235
NmuCI GTSAC 2 cut(s) 1127, 1259
NspI RCATGY 1 cut(s) 584
PaeI GCATGC 1 cut(s) 584
PagI TCATGA 1 cut(s) 903
PctI GAATGC 1 cut(s) 653
PfeI GAWTC 1 cut(s) 367
PfoI TCCNGGA 1 cut(s) 34
PkrI GCNGC 5 cut(s) 652, 847, 862, 886, 1180
Ple19I CGATCG 1 cut(s) 406
PleI GAGTC 4 cut(s) 610, 1112, 1124, 1211
PmaCI CACGTG 1 cut(s) 997
PmlI CACGTG 1 cut(s) 997
PpsI GAGTC 4 cut(s) 610, 1112, 1124, 1211
Ppu21I YACGTR 1 cut(s) 997
PpuMI RGGWCCY 1 cut(s) 668
Psp5II RGGWCCY 1 cut(s) 668
Psp6I CCWGG 2 cut(s) 715, 1021
PspCI CACGTG 1 cut(s) 997
PspFI CCCAGC 5 cut(s) 214, 383, 734, 989, 1238
PspGI CCWGG 2 cut(s) 715, 1021
PspN4I GGNNCC 4 cut(s) 670, 878, 1012, 1235
PspPI GGNCC 4 cut(s) 137, 668, 755, 1253
PspPPI RGGWCCY 1 cut(s) 668
PsuI RGATCY 2 cut(s) 1197, 1285
PvuI CGATCG 1 cut(s) 406
RsaI GTAC 2 cut(s) 811, 1235
RsaNI GTAC 2 cut(s) 810, 1234
SacII CCGCGG 1 cut(s) 271
SaqAI TTAA 3 cut(s) 723, 891, 1294
SatI GCNGC 5 cut(s) 651, 846, 861, 885, 1179
Sau3AI GATC 5 cut(s) 343, 403, 1036, 1197, 1285
Sau96I GGNCC 4 cut(s) 137, 668, 755, 1253
SchI GAGTC 4 cut(s) 610, 1112, 1124, 1211
ScrFI CCNGG 4 cut(s) 36, 206, 717, 1023
SduI GDGCHC 4 cut(s) 43, 881, 996, 1015
SfaNI GCATC 1 cut(s) 544
Sfr303I CCGCGG 1 cut(s) 271
SgrBI CCGCGG 1 cut(s) 271
SinI GGWCC 3 cut(s) 668, 755, 1253
SmlI CTYRAG 3 cut(s) 695, 854, 1132
SmoI CTYRAG 3 cut(s) 695, 854, 1132
SphI GCATGC 1 cut(s) 584
Sse9I AATT 4 cut(s) 141, 390, 1078, 1205
SsiI CCGC 5 cut(s) 268, 270, 837, 846, 861
SspI AATATT 1 cut(s) 727
SspMI CTAG 6 cut(s) 200, 288, 663, 731, 1040, 1058
StyD4I CCNGG 4 cut(s) 34, 204, 715, 1021
StyI CCWWGG 4 cut(s) 93, 99, 170, 1057
TaaI ACNGT 2 cut(s) 184, 362
TaiI ACGT 1 cut(s) 999
TaqI TCGA 2 cut(s) 630, 1153
TasI AATT 4 cut(s) 141, 390, 1078, 1205
TauI GCSGC 2 cut(s) 848, 863
TfiI GAWTC 1 cut(s) 367
Tru1I TTAA 3 cut(s) 723, 891, 1294
Tru9I TTAA 3 cut(s) 723, 891, 1294
TscAI CASTG 2 cut(s) 187, 648
TseFI GTSAC 2 cut(s) 1127, 1259
TseI GCWGC 3 cut(s) 650, 884, 1178
Tsp45I GTSAC 2 cut(s) 1127, 1259
TspDTI ATGAA 1 cut(s) 1091
TspRI CASTG 2 cut(s) 187, 648
VpaK11BI GGWCC 3 cut(s) 668, 755, 1253
XagI CCTNNNNNAGG 1 cut(s) 854
XbaI TCTAGA 1 cut(s) 662
XceI RCATGY 1 cut(s) 584
XmaJI CCTAGG 1 cut(s) 1057
XspI CTAG 6 cut(s) 200, 288, 663, 731, 1040, 1058
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.