FvH4_2g33340

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001634
Physical Location & Seq
Forward (+)
25041615 .. 25044176
2562 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33340.t1

Sequence Viewer

Length: 2322 bp
ATGGCACAAGTCGAGAAGATGAAAAATGTACCTGTTTTCATGCTTCTAGTAGTACTTCTGATTCTGCTTTTCTCTAACATCTGCGTCATAGCAGAAGAGAGAGATCAGAACATGAAGGAAACTTACATTATTCAGATGGACAAGTCCAACATACCAGCAACCTTCGACAATGATCATCACCAATGGTATGGTTCATCTTTAAAATCAGTGTCAGCTTCAGCCGAAGTGCTTTACACTTACCAAACTGTAGTTCACGGCTTCTCCAGCAGGCTCACGAGTGAGGAAGCTGAGCTGCTTGAAAAGCGGTCAGGTATTCTTTCTGTTCTACCTGAGATGAGATATGAACTTCATACAACTCGGACGTCGGAGTTTCTTGGGTTAACAGGAGAATACGAAGCTATCTTCCCAGCATCAGGCGATGTGAGTAACGTGGTGATCGGAGTTGTAGACACTGGTGTATGGCCTGAGAGCAAAAGCTACAATGACAAGGATCTTGCACCTGTGCCAAGGAGCTGGAAAGGGCAATGCGAGGACGCTAAGGGCTTCAACTCCTCAAGCTGCAATCGGAAACTGATTGGCGCCAGGTTTTTCTCAAAAGGGTATGAAGCAACACTTGGATCTGTGAACGAAAAAGTAGAATCAAGATCACCAAGAGATGATGACGGCCATGGAACTCACACCTCAAGCATTGCAGCTGGATCCCCTGTTCCAGGAGCTAGCCTCTTTGGTTACGCATCAGGGACAGCACGGGGAATGGCTGCACAAGCTCGTGTTGCCACATATAAGGCATGCTGGCATGGAGGGTGTTTCGGGTCCGATGTTCTAGCCGCAATGGACAAGGCTGTTCAAGATGGTGTCAACATTTTATCTCTGTCCTTGTCTTTTCCAGGAGCACAGTACGAGGATTTATCCACAGACGTTGTTGCTATTGGAGCTTTTTCTGCCATGGCCAGGGGAGTACTTGTGTCATGTTCAGCCGGAAATGATGGGCCATATTCCGATAGCTCATCCAACAATGCACCTTGGATAACCACTGTGGGTGCTGGAACACTAGACCGGGACTTCCCAGCTTATGTCAGTCTGGGAAATGGAAAAAAATACAGAGGTATATCACTCGATCATGGTACAGCACCATTACCCAATGGATTAGTTCCGCTTGTTTATGCTCCTAATGCAAGTAACTCCACTAGTGGTGATCAATGCGCTCCTAACAGTTTGATTCCTGAAATGGTTGCTGGGAAAATTGTGGTTTGTGATCGGGGAGGAAGTAGGGTCGGAAAGAGTGTGGTGGTAAAAAAGGCCGGTGGTGTAGGGATGATTTTGGCAAACACTGGAGATTATGGAGAAGAACTTGTGGCAGACGCATATCTCTTGCCAACTGCAATGGTTGGCAAGAAAGCTGGCGATGCAATAAAAAGATACATTGCCTCGCATGGTAATCCGAAAGCCATATTGGTTTTCGGAAAGACACAGTTGGGTATCAAACCCTCACCGGTGGTGACAGCATTCAGCTCTAGAGGACCGAATCCAGTCACTACATCAGTCCTCAAACCGGACTTGATAGCACCGGGAGTGAATATTCTAGCTGGGTGGACTCGTGCAGCTGGACCAACCGGACTACCTGAAGACAAAAGGAGGGTGAGCTACAACATCATTTCAGGTACATCCATGTCATGCCCTCATGTGAGCGGGCTTGCCGCCTTGCTCAAGGCCGCTCACCCAAAATGGAGCCCTGCTGCCATAAAATCTGCTCTCATGACCACATCCTATACAAGAAGAAATGGAAAACCAATCAAAGATGTTGCTACTGGAAAGCCTGCAACACCATTTGATTATGGAGCTGGGCATATGAACCCAGTCGCAGCCCTCCACCCAGGACTTATATATGATCTTGCTGTCAAGGACTACCTAGGCTTCCTCTGTGCCATGAATTACACAACAAAACATATCAAGAAACTCACTCACAAAGACTTCACCTGTGACTCGAGCAAGATATACAGAGTTGGAGATTTCAATTACCCATCTTTTGCTGCTACCATTTTAACCTCTGATGAAGGGGTCGGGGGTGGATCTGTCAATTACACAAGGACTCTAACCAATGTGGGTACCGCAGCAAGATACAAGGTCCGAGTGACTAAAAAATCCCCATTAGTGAAGATCTTGATTGAACCAAAATCATTGAGTTTCAGCCAAGCAAATGAGAAGAAGACTTGCACTGTGACTTTTATTGCCAGTTCTATGCCATCAACCAGCTTTGCTCACTTGGAATGGTTCGATGGGAAACACGTAGTAGGTAGTCCTATTGCTATCATGTGGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

774

Amino Acids

82.28

Weight (kDa)

8.85

Isoelectric Point (pI)

34.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 41 - 117 3.5e-16 Peptidase inhibitor I9
Peptidase_S8 PF00082 142 - 615 3e-44 Subtilase family
PA PF02225 385 - 471 1.9e-11 PA domain
fn3_6 PF17766 670 - 770 1.2e-20 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 365
Acc65I GGTACC 1 cut(s) 2105
AccB1I GGYRCC 2 cut(s) 578, 2105
AccBSI CCGCTC 2 cut(s) 1689, 1715
AccI GTMKAC 1 cut(s) 447
AciI CCGC 7 cut(s) 304, 828, 1154, 1689, 1698, 1713, 2109
AclWI GGATC 5 cut(s) 498, 625, 693, 706, 2077
AcoI YGGCCR 2 cut(s) 664, 948
AcuI CTGAAG 2 cut(s) 201, 1644
AcyI GRCGYC 2 cut(s) 362, 579
AfaI GTAC 7 cut(s) 30, 54, 899, 960, 1126, 1663, 2107
AfiI CCNNNNNNNGG 4 cut(s) 413, 710, 951, 1552
AflIII ACRYGT 1 cut(s) 2284
AgeI ACCGGT 1 cut(s) 1492
AgsI TTSAA 5 cut(s) 299, 547, 848, 2014, 2168
AhlI ACTAGT 1 cut(s) 1187
AjnI CCWGG 5 cut(s) 581, 709, 886, 950, 1873
AjuI GAANNNNNNNTTGG 6 cut(s) 234, 266, 597, 629, 1436, 1468
AloI GAACNNNNNNTCC 2 cut(s) 690, 722
Alw21I GWGCWC 1 cut(s) 895
AlwI GGATC 5 cut(s) 498, 625, 693, 706, 2077
Ama87I CYCGRG 1 cut(s) 1984
AoxI GGCC 6 cut(s) 461, 664, 948, 989, 1299, 1710
ApeKI GCWGC 9 cut(s) 292, 558, 692, 758, 1601, 1736, 1862, 2030, 2111
AsiGI ACCGGT 1 cut(s) 1492
Asp718I GGTACC 1 cut(s) 2105
AspA2I CCTAGG 1 cut(s) 1909
AspLEI GCGC 2 cut(s) 581, 1205
AspS9I GGNCC 5 cut(s) 813, 989, 1520, 1607, 2125
AsuC2I CCSGG 2 cut(s) 1058, 1569
AsuHPI GGTGA 9 cut(s) 170, 445, 639, 1205, 1482, 1510, 1651, 1709, 1966
AsuNHI GCTAGC 1 cut(s) 716
AvaI CYCGRG 1 cut(s) 1984
AvaII GGWCC 4 cut(s) 813, 1520, 1607, 2125
AvrII CCTAGG 1 cut(s) 1909
BalI TGGCCA 1 cut(s) 950
BamHI GGATCC 1 cut(s) 698
BanI GGYRCC 2 cut(s) 578, 2105
BanII GRGCYC 1 cut(s) 1733
BarI GAAGNNNNNNTAC 2 cut(s) 107, 139
BauI CACGAG 3 cut(s) 274, 768, 1596
BbsI GAAGAC 2 cut(s) 1632, 2213
Bbv12I GWGCWC 1 cut(s) 895
BbvI GCAGC 9 cut(s) 279, 545, 704, 745, 1613, 1723, 1874, 2017, 2123
BccI CCATC 6 cut(s) 130, 845, 980, 2029, 2251, 2270
BceAI ACGGC 2 cut(s) 271, 679
BciT130I CCWGG 5 cut(s) 583, 711, 888, 952, 1875
BclI TGATCA 2 cut(s) 172, 1195
BcnI CCSGG 2 cut(s) 1058, 1569
BcuI ACTAGT 1 cut(s) 1187
BfaI CTAG 8 cut(s) 47, 717, 824, 1052, 1188, 1515, 1583, 1910
BfmI CTRYAG 1 cut(s) 246
BfoI RGCGCY 1 cut(s) 582
BglII AGATCT 1 cut(s) 2157
BlnI CCTAGG 1 cut(s) 1909
BlpI GCTNAGC 1 cut(s) 288
BmcAI AGTACT 2 cut(s) 54, 960
Bme1390I CCNGG 7 cut(s) 583, 711, 888, 952, 1058, 1569, 1875
Bme18I GGWCC 4 cut(s) 813, 1520, 1607, 2125
BmeT110I CYCGRG 1 cut(s) 1984
BmgT120I GGNCC 5 cut(s) 813, 989, 1520, 1607, 2125
BmiI GGNNCC 5 cut(s) 580, 700, 814, 1730, 2107
BmrFI CCNGG 7 cut(s) 583, 711, 888, 952, 1058, 1569, 1875
BmrI ACTGGG 1 cut(s) 1850
BmsI GCATC 3 cut(s) 419, 743, 1396
BmtI GCTAGC 1 cut(s) 720
BmuI ACTGGG 1 cut(s) 1850
BpiI GAAGAC 2 cut(s) 1632, 2213
BplI GAGNNNNNCTC 2 cut(s) 705, 737
BpmI CTGGAG 2 cut(s) 247, 1353
Bpu10I CCTNAGC 1 cut(s) 537
Bpu1102I GCTNAGC 1 cut(s) 288
BpuEI CTTGAG 3 cut(s) 538, 667, 1691
BpuMI CCSGG 2 cut(s) 1058, 1569
BsaAI YACGTR 1 cut(s) 2287
BsaHI GRCGYC 2 cut(s) 362, 579
BsaJI CCNNGG 7 cut(s) 506, 667, 945, 951, 1022, 1873, 1909
BsaWI WCCGGW 3 cut(s) 1492, 1552, 1613
BsaXI ACNNNNNCTCC 4 cut(s) 245, 275, 948, 978
Bsc4I CCNNNNNNNGG 4 cut(s) 413, 710, 951, 1552
Bse118I RCCGGY 2 cut(s) 1301, 1492
Bse1I ACTGG 6 cut(s) 457, 1336, 1529, 1813, 1856, 2232
Bse3DI GCAATG 5 cut(s) 530, 687, 837, 1389, 1422
BseBI CCWGG 5 cut(s) 583, 711, 888, 952, 1875
BseDI CCNNGG 7 cut(s) 506, 667, 945, 951, 1022, 1873, 1909
BseGI GGATG 4 cut(s) 1007, 1320, 1664, 1763
BseLI CCNNNNNNNGG 4 cut(s) 413, 710, 951, 1552
BseMI GCAATG 5 cut(s) 530, 687, 837, 1389, 1422
BseMII CTCAG 3 cut(s) 279, 321, 456
BseNI ACTGG 6 cut(s) 457, 1336, 1529, 1813, 1856, 2232
BseRI GAGGAG 1 cut(s) 541
BseXI GCAGC 9 cut(s) 279, 545, 704, 745, 1613, 1723, 1874, 2017, 2123
BseYI CCCAGC 5 cut(s) 406, 1066, 1235, 1586, 1841
BsgI GTGCAG 2 cut(s) 744, 1620
BshFI GGCC 6 cut(s) 463, 666, 950, 991, 1301, 1712
BshNI GGYRCC 2 cut(s) 578, 2105
BshTI ACCGGT 1 cut(s) 1492
BsiHKAI GWGCWC 1 cut(s) 895
BsiHKCI CYCGRG 1 cut(s) 1984
BsiSI CCGG 7 cut(s) 978, 1057, 1302, 1493, 1553, 1568, 1614
BslFI GGGAC 2 cut(s) 754, 1073
BslI CCNNNNNNNGG 4 cut(s) 413, 710, 951, 1552
BsmFI GGGAC 2 cut(s) 754, 1073
BsmI GAATGC 1 cut(s) 1505
BsnI GGCC 6 cut(s) 463, 666, 950, 991, 1301, 1712
BsoBI CYCGRG 1 cut(s) 1984
Bsp1286I GDGCHC 2 cut(s) 895, 1733
Bsp1720I GCTNAGC 1 cut(s) 288
Bsp19I CCATGG 2 cut(s) 667, 945
BspACI CCGC 7 cut(s) 304, 828, 1154, 1689, 1698, 1713, 2109
BspANI GGCC 6 cut(s) 463, 666, 950, 991, 1301, 1712
BspCNI CTCAG 3 cut(s) 280, 322, 457
BspHI TCATGA 1 cut(s) 1755
BspLI GGNNCC 5 cut(s) 580, 700, 814, 1730, 2107
BspOI GCTAGC 1 cut(s) 720
BspPI GGATC 5 cut(s) 498, 625, 693, 706, 2077
BspT107I GGYRCC 2 cut(s) 578, 2105
BsrBI CCGCTC 2 cut(s) 1689, 1715
BsrDI GCAATG 5 cut(s) 530, 687, 837, 1389, 1422
BsrFI RCCGGY 2 cut(s) 1301, 1492
BsrI ACTGG 6 cut(s) 457, 1336, 1529, 1813, 1856, 2232
BssAI RCCGGY 2 cut(s) 1301, 1492
BssECI CCNNGG 7 cut(s) 506, 667, 945, 951, 1022, 1873, 1909
BssNI GRCGYC 2 cut(s) 362, 579
BssSI CACGAG 3 cut(s) 274, 768, 1596
BssT1I CCWWGG 5 cut(s) 506, 667, 945, 1022, 1909
Bst2BI CACGAG 3 cut(s) 274, 768, 1596
Bst2UI CCWGG 5 cut(s) 583, 711, 888, 952, 1875
Bst4CI ACNGT 6 cut(s) 247, 897, 1036, 1214, 1473, 2218
Bst6I CTCTTC 1 cut(s) 90
BstACI GRCGYC 2 cut(s) 362, 579
BstBAI YACGTR 1 cut(s) 2287
BstC8I GCNNGC 8 cut(s) 269, 718, 790, 794, 1402, 1691, 1695, 1818
BstDEI CTNAG 4 cut(s) 288, 330, 465, 537
BstDSI CCRYGG 2 cut(s) 667, 945
BstENI CCTNNNNNAGG 1 cut(s) 708
BstF5I GGATG 4 cut(s) 1007, 1320, 1664, 1763
BstH2I RGCGCY 1 cut(s) 582
BstHHI GCGC 2 cut(s) 581, 1205
BstMWI GCNNNNNNNGC 8 cut(s) 264, 301, 764, 773, 932, 941, 1172, 1406
BstNI CCWGG 5 cut(s) 583, 711, 888, 952, 1875
BstNSI RCATGY 1 cut(s) 792
BstSCI CCNGG 7 cut(s) 581, 709, 886, 950, 1056, 1567, 1873
BstSFI CTRYAG 1 cut(s) 246
BstV1I GCAGC 9 cut(s) 279, 545, 704, 745, 1613, 1723, 1874, 2017, 2123
BstV2I GAAGAC 2 cut(s) 1632, 2213
BstX2I RGATCY 5 cut(s) 490, 617, 698, 2069, 2157
BstXI CCANNNNNNTGG 2 cut(s) 188, 513
BstYI RGATCY 5 cut(s) 490, 617, 698, 2069, 2157
BsuRI GGCC 6 cut(s) 463, 666, 950, 991, 1301, 1712
BtgI CCRYGG 2 cut(s) 667, 945
BtgZI GCGATG 2 cut(s) 432, 1419
BtsCI GGATG 4 cut(s) 1007, 1320, 1664, 1763
BtsIMutI CAGTG 5 cut(s) 213, 450, 1032, 1329, 2214
Cac8I GCNNGC 8 cut(s) 269, 718, 790, 794, 1402, 1691, 1695, 1818
CciI TCATGA 1 cut(s) 1755
CfoI GCGC 2 cut(s) 581, 1205
Cfr10I RCCGGY 2 cut(s) 1301, 1492
Cfr13I GGNCC 5 cut(s) 813, 989, 1520, 1607, 2125
CseI GACGC 3 cut(s) 73, 542, 1370
Csp6I GTAC 7 cut(s) 29, 53, 898, 959, 1125, 1662, 2106
CspAI ACCGGT 1 cut(s) 1492
CspCI CAANNNNNGTGG 2 cut(s) 901, 936
CviQI GTAC 7 cut(s) 29, 53, 898, 959, 1125, 1662, 2106
DdeI CTNAG 4 cut(s) 288, 330, 465, 537
DinI GGCGCC 1 cut(s) 580
DraI TTTAAA 1 cut(s) 201
EaeI YGGCCR 2 cut(s) 664, 948
Eam1104I CTCTTC 1 cut(s) 90
EarI CTCTTC 1 cut(s) 90
Eco130I CCWWGG 5 cut(s) 506, 667, 945, 1022, 1909
Eco24I GRGCYC 1 cut(s) 1733
Eco47I GGWCC 4 cut(s) 813, 1520, 1607, 2125
Eco57I CTGAAG 2 cut(s) 201, 1644
Eco88I CYCGRG 1 cut(s) 1984
EcoNI CCTNNNNNAGG 1 cut(s) 708
EcoRII CCWGG 5 cut(s) 581, 709, 886, 950, 1873
EcoT14I CCWWGG 5 cut(s) 506, 667, 945, 1022, 1909
EcoT38I GRGCYC 1 cut(s) 1733
EgeI GGCGCC 1 cut(s) 580
EheI GGCGCC 1 cut(s) 580
ErhI CCWWGG 5 cut(s) 506, 667, 945, 1022, 1909
FalI AAGNNNNNCTT 2 cut(s) 597, 629
FaqI GGGAC 2 cut(s) 754, 1073
FauI CCCGC 1 cut(s) 1682
FauNDI CATATG 1 cut(s) 1848
FbaI TGATCA 2 cut(s) 172, 1195
FblI GTMKAC 1 cut(s) 447
FokI GGATG 4 cut(s) 994, 1327, 1651, 1750
FriOI GRGCYC 1 cut(s) 1733
FspBI CTAG 8 cut(s) 47, 717, 824, 1052, 1188, 1515, 1583, 1910
GlaI GCGC 2 cut(s) 580, 1204
GsaI CCCAGC 5 cut(s) 410, 1070, 1239, 1590, 1845
GsuI CTGGAG 2 cut(s) 247, 1353
HaeII RGCGCY 1 cut(s) 582
HaeIII GGCC 6 cut(s) 463, 666, 950, 991, 1301, 1712
HapII CCGG 7 cut(s) 978, 1057, 1302, 1493, 1553, 1568, 1614
HgaI GACGC 3 cut(s) 73, 542, 1370
HhaI GCGC 2 cut(s) 581, 1205
Hin1I GRCGYC 2 cut(s) 362, 579
Hin6I GCGC 2 cut(s) 579, 1203
HinP1I GCGC 2 cut(s) 579, 1203
HincII GTYRAC 2 cut(s) 381, 859
HindII GTYRAC 2 cut(s) 381, 859
HinfI GANTC 7 cut(s) 61, 638, 1219, 1525, 1594, 1982, 2089
HpaI GTTAAC 1 cut(s) 381
HpaII CCGG 7 cut(s) 978, 1057, 1302, 1493, 1553, 1568, 1614
HphI GGTGA 9 cut(s) 170, 445, 639, 1205, 1482, 1510, 1651, 1709, 1966
Hpy166II GTNNAC 6 cut(s) 253, 381, 448, 625, 859, 1593
Hpy188III TCNNGA 9 cut(s) 13, 274, 642, 848, 1223, 1515, 1756, 1951, 2161
Hpy8I GTNNAC 6 cut(s) 253, 381, 448, 625, 859, 1593
Hpy99I CGWCG 1 cut(s) 367
HpyAV CCTTC 3 cut(s) 109, 172, 2048
HpyCH4III ACNGT 6 cut(s) 247, 897, 1036, 1214, 1473, 2218
HpyCH4IV ACGT 4 cut(s) 362, 429, 918, 2286
HpyF10VI GCNNNNNNNGC 8 cut(s) 264, 301, 764, 773, 932, 941, 1172, 1406
HpyF3I CTNAG 4 cut(s) 288, 330, 465, 537
HpySE526I ACGT 4 cut(s) 362, 429, 918, 2286
Hsp92I GRCGYC 2 cut(s) 362, 579
HspAI GCGC 2 cut(s) 579, 1203
KasI GGCGCC 1 cut(s) 578
KpnI GGTACC 1 cut(s) 2109
Ksp22I TGATCA 2 cut(s) 172, 1195
KspAI GTTAAC 1 cut(s) 381
LmnI GCTCC 8 cut(s) 510, 713, 890, 932, 1171, 1210, 1728, 1838
Lsp1109I GCAGC 9 cut(s) 279, 545, 704, 745, 1613, 1723, 1874, 2017, 2123
LweI GCATC 3 cut(s) 419, 743, 1396
MaeI CTAG 8 cut(s) 47, 717, 824, 1052, 1188, 1515, 1583, 1910
MaeII ACGT 4 cut(s) 362, 429, 918, 2286
MaeIII GTNAC 8 cut(s) 425, 728, 1178, 1498, 1531, 1979, 2131, 2218
MbiI CCGCTC 2 cut(s) 1689, 1715
MboII GAAGA 9 cut(s) 28, 107, 394, 1358, 1637, 1788, 2167, 2215, 2218
MflI RGATCY 5 cut(s) 490, 617, 698, 2069, 2157
MhlI GDGCHC 2 cut(s) 895, 1733
MlsI TGGCCA 1 cut(s) 950
MluCI AATT 4 cut(s) 1242, 1930, 2014, 2077
MluNI TGGCCA 1 cut(s) 950
Mly113I GGCGCC 1 cut(s) 579
MlyI GAGTC 3 cut(s) 1588, 1976, 2083
MmeI TCCRAC 5 cut(s) 171, 345, 1035, 1255, 1984
Mox20I TGGCCA 1 cut(s) 950
MscI TGGCCA 1 cut(s) 950
MseI TTAA 3 cut(s) 200, 380, 2042
Msp20I TGGCCA 1 cut(s) 950
MspA1I CMGCKG 2 cut(s) 695, 1604
MspI CCGG 7 cut(s) 978, 1057, 1302, 1493, 1553, 1568, 1614
MspR9I CCNGG 7 cut(s) 583, 711, 888, 952, 1058, 1569, 1875
Mva1269I GAATGC 1 cut(s) 1505
MvaI CCWGG 5 cut(s) 583, 711, 888, 952, 1875
MwoI GCNNNNNNNGC 8 cut(s) 264, 301, 764, 773, 932, 941, 1172, 1406
NarI GGCGCC 1 cut(s) 579
NciI CCSGG 2 cut(s) 1058, 1569
NcoI CCATGG 2 cut(s) 667, 945
NdeI CATATG 1 cut(s) 1848
NheI GCTAGC 1 cut(s) 716
NlaIV GGNNCC 5 cut(s) 580, 700, 814, 1730, 2107
NmuCI GTSAC 5 cut(s) 1498, 1531, 1979, 2131, 2218
NspI RCATGY 1 cut(s) 792
PaeI GCATGC 1 cut(s) 792
PaeR7I CTCGAG 1 cut(s) 1984
PagI TCATGA 1 cut(s) 1755
PctI GAATGC 1 cut(s) 1505
PfeI GAWTC 4 cut(s) 61, 638, 1219, 1525
PfoI TCCNGGA 2 cut(s) 709, 886
PinAI ACCGGT 1 cut(s) 1492
PleI GAGTC 3 cut(s) 1588, 1976, 2083
PluTI GGCGCC 1 cut(s) 582
PpsI GAGTC 3 cut(s) 1588, 1976, 2083
Ppu21I YACGTR 1 cut(s) 2287
Psp6I CCWGG 5 cut(s) 581, 709, 886, 950, 1873
PspFI CCCAGC 5 cut(s) 406, 1066, 1235, 1586, 1841
PspGI CCWGG 5 cut(s) 581, 709, 886, 950, 1873
PspN4I GGNNCC 5 cut(s) 580, 700, 814, 1730, 2107
PspPI GGNCC 5 cut(s) 813, 989, 1520, 1607, 2125
PspXI VCTCGAGB 1 cut(s) 1984
PsuI RGATCY 5 cut(s) 490, 617, 698, 2069, 2157
PvuII CAGCTG 2 cut(s) 695, 1604
RsaI GTAC 7 cut(s) 30, 54, 899, 960, 1126, 1663, 2107
RsaNI GTAC 7 cut(s) 29, 53, 898, 959, 1125, 1662, 2106
SaqAI TTAA 3 cut(s) 200, 380, 2042
Sau96I GGNCC 5 cut(s) 813, 989, 1520, 1607, 2125
ScaI AGTACT 2 cut(s) 54, 960
SchI GAGTC 3 cut(s) 1588, 1976, 2083
ScrFI CCNGG 7 cut(s) 583, 711, 888, 952, 1058, 1569, 1875
SduI GDGCHC 2 cut(s) 895, 1733
SfaNI GCATC 3 cut(s) 419, 743, 1396
SfcI CTRYAG 1 cut(s) 246
SfoI GGCGCC 1 cut(s) 580
Sfr274I CTCGAG 1 cut(s) 1984
SgrAI CRCCGGYG 1 cut(s) 1492
SinI GGWCC 4 cut(s) 813, 1520, 1607, 2125
SlaI CTCGAG 1 cut(s) 1984
SmlI CTYRAG 4 cut(s) 553, 682, 1706, 1984
SmoI CTYRAG 4 cut(s) 553, 682, 1706, 1984
SpeI ACTAGT 1 cut(s) 1187
SphI GCATGC 1 cut(s) 792
Sse9I AATT 4 cut(s) 1242, 1930, 2014, 2077
SsiI CCGC 7 cut(s) 304, 828, 1154, 1689, 1698, 1713, 2109
SspDI GGCGCC 1 cut(s) 578
SspI AATATT 1 cut(s) 1579
SspMI CTAG 8 cut(s) 47, 717, 824, 1052, 1188, 1515, 1583, 1910
StyD4I CCNGG 7 cut(s) 581, 709, 886, 950, 1056, 1567, 1873
StyI CCWWGG 5 cut(s) 506, 667, 945, 1022, 1909
TaaI ACNGT 6 cut(s) 247, 897, 1036, 1214, 1473, 2218
TaiI ACGT 4 cut(s) 365, 432, 921, 2289
TaqI TCGA 5 cut(s) 12, 165, 1116, 1985, 2274
TaqII GACCGA 1 cut(s) 1537
TasI AATT 4 cut(s) 1242, 1930, 2014, 2077
TatI WGTACW 2 cut(s) 52, 958
TauI GCSGC 3 cut(s) 830, 1700, 1715
TfiI GAWTC 4 cut(s) 61, 638, 1219, 1525
Tru1I TTAA 3 cut(s) 200, 380, 2042
Tru9I TTAA 3 cut(s) 200, 380, 2042
TscAI CASTG 5 cut(s) 213, 457, 1039, 1336, 2221
TseFI GTSAC 5 cut(s) 1498, 1531, 1979, 2131, 2218
TseI GCWGC 9 cut(s) 292, 558, 692, 758, 1601, 1736, 1862, 2030, 2111
Tsp45I GTSAC 5 cut(s) 1498, 1531, 1979, 2131, 2218
TspRI CASTG 5 cut(s) 213, 457, 1039, 1336, 2221
VpaK11BI GGWCC 4 cut(s) 813, 1520, 1607, 2125
XagI CCTNNNNNAGG 1 cut(s) 708
XbaI TCTAGA 1 cut(s) 1514
XceI RCATGY 1 cut(s) 792
XhoI CTCGAG 1 cut(s) 1984
XmaJI CCTAGG 1 cut(s) 1909
XmiI GTMKAC 1 cut(s) 447
XspI CTAG 8 cut(s) 47, 717, 824, 1052, 1188, 1515, 1583, 1910
ZraI GACGTC 1 cut(s) 363
ZrmI AGTACT 2 cut(s) 54, 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.