MD08G1087900.v1.1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001634
Physical Location & Seq
Forward (+)
7268429 .. 7270744
2316 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1087900.v1.1.491

Sequence Viewer

Length: 2316 bp
ATGAAGATATCCAGTTTCATGCTTCTGCTACCAGTCATGCTTCTTGCTCTCTCTCGCATCTCCGCTATCGTAGCGGAAGACGCGAGAGAGCAGCAGGTGAAAAAGACTTACATCATTCACATGGACAAGTCCGAAATGCCGGCGAGTTTTGAAGATGATCATTTCCAATGGTATGATTCTTCTTTGAAATCCGTGTCGAGTTCAGCCGACATGCTTTACACTTACAAGACCATAATCCACGGCTTCGCCACCAGGCTAACCGCCGAGGAAGCTGAGCTGCTTGGGAAGCAGTCGGGAATTCTGTCCGTTCTGCCCGAAAGGAGGTACGAGCTGCATACAACTCGGACGCCCGAGTTTCTTGGATTAGGAAAGAGTGAAGCCCTCTTGCCGGCGTCGGGTAAAGTGAGTGAAGTGATTGTCGGAGTGGTGGACACTGGCGTGTGGCCTGAGCTCAAAAGCTACGACGACACAGGGCTCGCGGCGGTACCAAGTGGCTGGAAAGGGGTGTGTGAGGCGGGAACAAACTTCAGCTCCTCAAACTGCAACCGGAAACTCATAGGCGCGAGGTTTTTCTCGAAAGGGTATGAAGCTACAGTTGGACCAATCGATGTGAAAACAGAATCGAAATCACCACGAGATGATGATGGCCACGGAACCCACACCTCATCCACTGCAACCGGTTCCGCAGTTTCAGGAGCTAGCCTCTTTGGTTATGCTTCGGGGACAGCGCGAGGGATGGCGCCACAAGCTAGATTGGCCGCGTACAAGGCGTGCTGGCTTGGTGGGTGTTTCGGCTCTGATATCACAGCTGCAATGGAGAAGGCCGTGGAAGACGGTGTCGATGTTTTATCTTTGTCTATTGGGGGATCACAGTCTGAATATTACAGAGACACTGTTGCTATTGGAGCTTTCTCTGCCGCGGCGCAGGGGATCCTTGTGTCGTGTTCAGCTGGTAATGGAGGACCGGAAGCAGGGAGCCTGTCCAACGTTGCGCCTTGGATAACTACAGTGGGTGCTGGAACATTAGACAGGGATTTCCCGGCTTTTGTTAACCTCGGAAGTGCAAAGAAATACAGAGGTATATCACTGTATAGGGGAACATCCTTATCTAGGGGATTGCTTCCGCTTGTATATGCTGGCAATGCAAGTGTCTCCTCCAGTGGCGGGCTATGCGCTCCTGAAAGTCTGACTCCCTCAAAGGTTGCAGGGAAAATTGTGGTATGTGATCGAGGGGGAACCCCTAGGGTCCAAAAGAGTTTGGTGGTGAAAAAGGCCGGCGGTTTAGGGATGATTTTGACGAACACTGATGCTTACGGAGAGGAACTTGTTGCGGATGCGTATCTACTGCCTACAGCAGCGGTTGGTGCGAAAGCTGGTGCTGCAATAAAAAGCTATATTGCCTCGCAATCTAATCCTACAGGCACAATTGCGCTTGGAGACACGGAGTTAGATGTGCAGCCCTCTCCCGTGGTGGCATCATTCAGCTCAAGAGGACCGAACCTAGTCACCCCGGAAATACTCAAACCAGACCTAATAGCACCGGGAGTAAATATCCTTGCTGGGTGGACTGGTGCGATTGGACCAACCGGACTAACCGAAGACAAGAGGCATGTCACCTTCAACATCATTTCGGGTACTTCCATGTCATGCCCGCATGTGAGTGGTTTGGCCGCCCTTGTCAAGGCCGCTCATCCAGAATGGAGCCCTGCAGCCATTAAGTCTGCACTCATGACTACATCCTACACAGCATACAAAACTGGAGAAACCATCATAGATGTAGCATCTGGAAATCCTGCAACGCCGTTTGATTATGGTGCTGGGCATGTGGATCCGATGGCAGCCCTCGACCCTGGCCTTGTTTATGATAATGCGGTCGAGGACTACTTCAGCTTCCTCTGCGCCTTGAATTATAGCTCAACTGAGATCAAACTAACCACTCACAGAGACTTCACTTGCGATACAAGCAAGAAATACAGGCTTGGAGATTTCAACTACCCGTCTTTTGCTGTTCCCCTAGAAACATCTTCTGGCACAGGGGGTGGAACAGGTGCTTCAACAACTGTAAAATACACAAGGACACTGACCAATGTGGGTACCCCAGGAACATACAAGGTCACGATAACTTCACAGGCGCCATCAGTGAAGATCTCGGTTGAGCCACAATCACTGACTTTCAGTCAAGCATACGAGAAGAAAACATATACCGTGACTTTTGTTGCTGGTTCTTCACCATCCGGTACAAGCAGCTTTGCTCGGTTGGTATGGTCCGATGGGAAACGCACAGTAGGCAGTCCAATTGCTTTCACCTGGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

772

Amino Acids

80.69

Weight (kDa)

6.3

Isoelectric Point (pI)

31.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 36 - 112 4.6e-18 Peptidase inhibitor I9
Peptidase_S8 PF00082 137 - 607 1.6e-47 Subtilase family
PA PF02225 375 - 462 1.9e-12 PA domain
fn3_6 PF17766 662 - 767 2.6e-24 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 85
Acc36I ACCTGC 1 cut(s) 85
Acc65I GGTACC 2 cut(s) 484, 2093
AccB1I GGYRCC 4 cut(s) 484, 739, 2093, 2131
AccBSI CCGCTC 1 cut(s) 1688
AccII CGCG 6 cut(s) 83, 479, 563, 730, 761, 920
AclI AACGTT 1 cut(s) 987
AclWI GGATC 5 cut(s) 874, 925, 938, 1823, 1836
AcoI YGGCCR 3 cut(s) 646, 756, 1668
AcsI RAATTY 1 cut(s) 297
AcuI CTGAAG 2 cut(s) 511, 1870
AcyI GRCGYC 4 cut(s) 347, 392, 740, 2132
AfaI GTAC 6 cut(s) 326, 486, 764, 1636, 2095, 2239
AfiI CCNNNNNNNGG 7 cut(s) 321, 388, 395, 971, 1110, 1164, 1681
AgeI ACCGGT 1 cut(s) 677
AgsI TTSAA 6 cut(s) 152, 187, 1621, 1906, 1990, 2055
AhdI GACNNNNNGTC 1 cut(s) 2175
AjnI CCWGG 4 cut(s) 251, 1849, 2098, 2306
AjuI GAANNNNNNNTTGG 2 cut(s) 579, 611
AleI CACNNNNGTG 1 cut(s) 437
Alw21I GWGCWC 1 cut(s) 453
Alw26I GTCTC 4 cut(s) 882, 1156, 1431, 1938
AlwI GGATC 5 cut(s) 874, 925, 938, 1823, 1836
Ama87I CYCGRG 1 cut(s) 350
AoxI GGCC 8 cut(s) 443, 646, 756, 822, 1272, 1668, 1683, 1852
ApoI RAATTY 1 cut(s) 297
AsiGI ACCGGT 1 cut(s) 677
Asp718I GGTACC 2 cut(s) 484, 2093
AspA2I CCTAGG 1 cut(s) 1241
AspLEI GCGC 9 cut(s) 563, 730, 742, 925, 994, 1175, 1432, 1901, 2134
AspS9I GGNCC 6 cut(s) 599, 962, 1246, 1493, 1580, 2265
AsuC2I CCSGG 3 cut(s) 1040, 1511, 1542
AsuHPI GGTGA 7 cut(s) 109, 621, 1276, 1498, 1606, 2220, 2296
AsuNHI GCTAGC 1 cut(s) 698
AvaI CYCGRG 1 cut(s) 350
AvaII GGWCC 6 cut(s) 599, 962, 1246, 1493, 1580, 2265
AvrII CCTAGG 1 cut(s) 1241
BalI TGGCCA 1 cut(s) 648
BamHI GGATCC 2 cut(s) 930, 1828
BanI GGYRCC 4 cut(s) 484, 739, 2093, 2131
BanII GRGCYC 3 cut(s) 453, 477, 1706
BauI CACGAG 1 cut(s) 633
BbsI GAAGAC 3 cut(s) 84, 837, 1605
Bbv12I GWGCWC 1 cut(s) 453
BccI CCATC 7 cut(s) 638, 730, 1775, 1828, 2143, 2239, 2264
BceAI ACGGC 3 cut(s) 256, 809, 1786
BcgI CGANNNNNNTGC 2 cut(s) 323, 357
BciT130I CCWGG 4 cut(s) 253, 1851, 2100, 2308
BclI TGATCA 1 cut(s) 157
BcnI CCSGG 3 cut(s) 1040, 1511, 1542
BcoDI GTCTC 4 cut(s) 882, 1156, 1431, 1938
BfaI CTAG 6 cut(s) 699, 750, 1110, 1242, 1502, 2015
BfmI CTRYAG 5 cut(s) 591, 1005, 1350, 1416, 1707
BfoI RGCGCY 2 cut(s) 743, 2135
BfuAI ACCTGC 1 cut(s) 85
BglII AGATCT 1 cut(s) 2145
BlnI CCTAGG 1 cut(s) 1241
BlpI GCTNAGC 1 cut(s) 273
Bme1390I CCNGG 7 cut(s) 253, 1040, 1511, 1542, 1851, 2100, 2308
Bme18I GGWCC 6 cut(s) 599, 962, 1246, 1493, 1580, 2265
BmeRI GACNNNNNGTC 1 cut(s) 2175
BmeT110I CYCGRG 1 cut(s) 350
BmgT120I GGNCC 6 cut(s) 599, 962, 1246, 1493, 1580, 2265
BmrFI CCNGG 7 cut(s) 253, 1040, 1511, 1542, 1851, 2100, 2308
BmsI GCATC 5 cut(s) 66, 1297, 1324, 1484, 1790
BmtI GCTAGC 1 cut(s) 702
BpiI GAAGAC 3 cut(s) 84, 837, 1605
BplI GAGNNNNNCTC 2 cut(s) 687, 719
BpmI CTGGAG 2 cut(s) 1141, 1779
Bpu10I CCTNAGC 1 cut(s) 447
Bpu1102I GCTNAGC 1 cut(s) 273
BpuEI CTTGAG 1 cut(s) 1471
BpuMI CCSGG 3 cut(s) 1040, 1511, 1542
Bsa29I ATCGAT 1 cut(s) 606
BsaBI GATNNNNATC 1 cut(s) 1338
BsaHI GRCGYC 4 cut(s) 347, 392, 740, 2132
BsaWI WCCGGW 5 cut(s) 546, 677, 964, 1586, 2234
BsaXI ACNNNNNCTCC 2 cut(s) 515, 545
Bsc4I CCNNNNNNNGG 7 cut(s) 321, 388, 395, 971, 1110, 1164, 1681
Bse118I RCCGGY 4 cut(s) 139, 388, 677, 1274
Bse1I ACTGG 6 cut(s) 12, 32, 439, 1158, 1573, 1762
Bse3DI GCAATG 2 cut(s) 819, 1147
Bse8I GATNNNNATC 1 cut(s) 1338
BseBI CCWGG 4 cut(s) 253, 1851, 2100, 2308
BseCI ATCGAT 1 cut(s) 606
BseGI GGATG 8 cut(s) 665, 741, 1100, 1293, 1339, 1690, 1736, 2231
BseJI GATNNNNATC 1 cut(s) 1338
BseLI CCNNNNNNNGG 7 cut(s) 321, 388, 395, 971, 1110, 1164, 1681
BseMI GCAATG 2 cut(s) 819, 1147
BseMII CTCAG 3 cut(s) 264, 438, 1911
BseNI ACTGG 6 cut(s) 12, 32, 439, 1158, 1573, 1762
BseRI GAGGAG 2 cut(s) 523, 1144
BseYI CCCAGC 2 cut(s) 1559, 1817
BsgI GTGCAG 2 cut(s) 1475, 1707
Bsh1236I CGCG 6 cut(s) 83, 479, 563, 730, 761, 920
Bsh1285I CGRYCG 1 cut(s) 1875
BshFI GGCC 8 cut(s) 445, 648, 758, 824, 1274, 1670, 1685, 1854
BshNI GGYRCC 4 cut(s) 484, 739, 2093, 2131
BshTI ACCGGT 1 cut(s) 677
BshVI ATCGAT 1 cut(s) 606
BsiEI CGRYCG 1 cut(s) 1875
BsiHKAI GWGCWC 1 cut(s) 453
BsiHKCI CYCGRG 1 cut(s) 350
BslFI GGGAC 1 cut(s) 736
BslI CCNNNNNNNGG 7 cut(s) 321, 388, 395, 971, 1110, 1164, 1681
BsmAI GTCTC 4 cut(s) 882, 1156, 1431, 1938
BsmFI GGGAC 1 cut(s) 736
BsnI GGCC 8 cut(s) 445, 648, 758, 824, 1274, 1670, 1685, 1854
BsoBI CYCGRG 1 cut(s) 350
Bsp1286I GDGCHC 3 cut(s) 453, 477, 1706
Bsp143I GATC 7 cut(s) 157, 866, 930, 1225, 1828, 1923, 2145
Bsp1720I GCTNAGC 1 cut(s) 273
BspANI GGCC 8 cut(s) 445, 648, 758, 824, 1274, 1670, 1685, 1854
BspCNI CTCAG 3 cut(s) 265, 439, 1912
BspDI ATCGAT 1 cut(s) 606
BspFNI CGCG 6 cut(s) 83, 479, 563, 730, 761, 920
BspHI TCATGA 1 cut(s) 1728
BspMAI CTGCAG 1 cut(s) 1711
BspMI ACCTGC 1 cut(s) 85
BspOI GCTAGC 1 cut(s) 702
BspPI GGATC 5 cut(s) 874, 925, 938, 1823, 1836
BspT107I GGYRCC 4 cut(s) 484, 739, 2093, 2131
BsrBI CCGCTC 1 cut(s) 1688
BsrDI GCAATG 2 cut(s) 819, 1147
BsrFI RCCGGY 4 cut(s) 139, 388, 677, 1274
BsrI ACTGG 6 cut(s) 12, 32, 439, 1158, 1573, 1762
BssAI RCCGGY 4 cut(s) 139, 388, 677, 1274
BssMI GATC 7 cut(s) 157, 866, 930, 1225, 1828, 1923, 2145
BssNI GRCGYC 4 cut(s) 347, 392, 740, 2132
BssSI CACGAG 1 cut(s) 633
BssT1I CCWWGG 2 cut(s) 995, 1241
Bst2BI CACGAG 1 cut(s) 633
Bst2UI CCWGG 4 cut(s) 253, 1851, 2100, 2308
Bst4CI ACNGT 9 cut(s) 595, 836, 873, 895, 1009, 1089, 2062, 2206, 2284
BstACI GRCGYC 4 cut(s) 347, 392, 740, 2132
BstDEI CTNAG 3 cut(s) 273, 447, 1920
BstDSI CCRYGG 5 cut(s) 238, 649, 825, 918, 1467
BstENI CCTNNNNNAGG 2 cut(s) 1108, 1679
BstF5I GGATG 8 cut(s) 665, 741, 1100, 1293, 1339, 1690, 1736, 2231
BstFNI CGCG 6 cut(s) 83, 479, 563, 730, 761, 920
BstH2I RGCGCY 2 cut(s) 743, 2135
BstHHI GCGC 9 cut(s) 563, 730, 742, 925, 994, 1175, 1432, 1901, 2134
BstKTI GATC 7 cut(s) 160, 869, 933, 1228, 1831, 1926, 2148
BstMAI GTCTC 4 cut(s) 882, 1156, 1431, 1938
BstMBI GATC 7 cut(s) 157, 866, 930, 1225, 1828, 1923, 2145
BstMCI CGRYCG 1 cut(s) 1875
BstNI CCWGG 4 cut(s) 253, 1851, 2100, 2308
BstNSI RCATGY 4 cut(s) 214, 1613, 1658, 1826
BstSCI CCNGG 7 cut(s) 251, 1038, 1509, 1540, 1849, 2098, 2306
BstSFI CTRYAG 5 cut(s) 591, 1005, 1350, 1416, 1707
BstUI CGCG 6 cut(s) 83, 479, 563, 730, 761, 920
BstV2I GAAGAC 3 cut(s) 84, 837, 1605
BstX2I RGATCY 3 cut(s) 930, 1828, 2145
BstXI CCANNNNNNTGG 1 cut(s) 495
BstYI RGATCY 3 cut(s) 930, 1828, 2145
Bsu15I ATCGAT 1 cut(s) 606
BsuRI GGCC 8 cut(s) 445, 648, 758, 824, 1274, 1670, 1685, 1854
BsuTUI ATCGAT 1 cut(s) 606
BtgI CCRYGG 5 cut(s) 238, 649, 825, 918, 1467
BtsCI GGATG 8 cut(s) 665, 741, 1100, 1293, 1339, 1690, 1736, 2231
BtsI GCAGTG 1 cut(s) 669
BveI ACCTGC 1 cut(s) 85
CciI TCATGA 1 cut(s) 1728
CfoI GCGC 9 cut(s) 563, 730, 742, 925, 994, 1175, 1432, 1901, 2134
Cfr10I RCCGGY 4 cut(s) 139, 388, 677, 1274
Cfr13I GGNCC 6 cut(s) 599, 962, 1246, 1493, 1580, 2265
Cfr42I CCGCGG 1 cut(s) 921
ClaI ATCGAT 1 cut(s) 606
CseI GACGC 3 cut(s) 89, 355, 381
Csp6I GTAC 6 cut(s) 325, 485, 763, 1635, 2094, 2238
CspAI ACCGGT 1 cut(s) 677
CviQI GTAC 6 cut(s) 325, 485, 763, 1635, 2094, 2238
DdeI CTNAG 3 cut(s) 273, 447, 1920
DinI GGCGCC 2 cut(s) 741, 2133
DpnI GATC 7 cut(s) 159, 868, 932, 1227, 1830, 1925, 2147
DpnII GATC 7 cut(s) 157, 866, 930, 1225, 1828, 1923, 2145
DriI GACNNNNNGTC 1 cut(s) 2175
EaeI YGGCCR 3 cut(s) 646, 756, 1668
Eam1105I GACNNNNNGTC 1 cut(s) 2175
Ecl136II GAGCTC 1 cut(s) 451
Eco130I CCWWGG 2 cut(s) 995, 1241
Eco24I GRGCYC 3 cut(s) 453, 477, 1706
Eco32I GATATC 2 cut(s) 9, 802
Eco47I GGWCC 6 cut(s) 599, 962, 1246, 1493, 1580, 2265
Eco53kI GAGCTC 1 cut(s) 451
Eco57I CTGAAG 2 cut(s) 511, 1870
Eco88I CYCGRG 1 cut(s) 350
EcoICRI GAGCTC 1 cut(s) 451
EcoNI CCTNNNNNAGG 2 cut(s) 1108, 1679
EcoRI GAATTC 1 cut(s) 297
EcoRII CCWGG 4 cut(s) 251, 1849, 2098, 2306
EcoRV GATATC 2 cut(s) 9, 802
EcoT14I CCWWGG 2 cut(s) 995, 1241
EcoT38I GRGCYC 3 cut(s) 453, 477, 1706
EgeI GGCGCC 2 cut(s) 741, 2133
EheI GGCGCC 2 cut(s) 741, 2133
ErhI CCWWGG 2 cut(s) 995, 1241
FaqI GGGAC 1 cut(s) 736
FauI CCCGC 3 cut(s) 508, 1157, 1659
FbaI TGATCA 1 cut(s) 157
FokI GGATG 8 cut(s) 652, 748, 1087, 1300, 1346, 1677, 1723, 2218
FriOI GRGCYC 3 cut(s) 453, 477, 1706
FspBI CTAG 6 cut(s) 699, 750, 1110, 1242, 1502, 2015
GlaI GCGC 9 cut(s) 562, 729, 741, 924, 993, 1174, 1431, 1900, 2133
GsaI CCCAGC 2 cut(s) 1563, 1821
GsuI CTGGAG 2 cut(s) 1141, 1779
HaeII RGCGCY 2 cut(s) 743, 2135
HaeIII GGCC 8 cut(s) 445, 648, 758, 824, 1274, 1670, 1685, 1854
HgaI GACGC 3 cut(s) 89, 355, 381
HhaI GCGC 9 cut(s) 563, 730, 742, 925, 994, 1175, 1432, 1901, 2134
Hin1I GRCGYC 4 cut(s) 347, 392, 740, 2132
Hin6I GCGC 9 cut(s) 561, 728, 740, 923, 992, 1173, 1430, 1899, 2132
HinP1I GCGC 9 cut(s) 561, 728, 740, 923, 992, 1173, 1430, 1899, 2132
HincII GTYRAC 1 cut(s) 1051
HindII GTYRAC 1 cut(s) 1051
HinfI GANTC 3 cut(s) 176, 620, 1189
HpaI GTTAAC 1 cut(s) 1051
HphI GGTGA 7 cut(s) 109, 621, 1276, 1498, 1606, 2220, 2296
Hpy166II GTNNAC 3 cut(s) 430, 1051, 1566
Hpy188I TCNGA 9 cut(s) 133, 345, 422, 799, 877, 1058, 1188, 1833, 2269
Hpy188III TCNNGA 9 cut(s) 294, 574, 693, 1178, 1488, 1694, 1729, 1785, 2116
Hpy8I GTNNAC 3 cut(s) 430, 1051, 1566
Hpy99I CGWCG 2 cut(s) 397, 467
HpyAV CCTTC 2 cut(s) 814, 1627
HpyCH4III ACNGT 9 cut(s) 595, 836, 873, 895, 1009, 1089, 2062, 2206, 2284
HpyCH4IV ACGT 1 cut(s) 987
HpyF3I CTNAG 3 cut(s) 273, 447, 1920
HpySE526I ACGT 1 cut(s) 987
Hsp92I GRCGYC 4 cut(s) 347, 392, 740, 2132
HspAI GCGC 9 cut(s) 561, 728, 740, 923, 992, 1173, 1430, 1899, 2132
KasI GGCGCC 2 cut(s) 739, 2131
KpnI GGTACC 2 cut(s) 488, 2097
KroI GCCGGC 3 cut(s) 139, 388, 1274
KroNI GCCGGC 3 cut(s) 141, 390, 1276
Ksp22I TGATCA 1 cut(s) 157
KspAI GTTAAC 1 cut(s) 1051
KspI CCGCGG 1 cut(s) 921
Kzo9I GATC 7 cut(s) 157, 866, 930, 1225, 1828, 1923, 2145
LmnI GCTCC 6 cut(s) 536, 695, 905, 975, 1180, 1701
LweI GCATC 5 cut(s) 66, 1297, 1324, 1484, 1790
MaeI CTAG 6 cut(s) 699, 750, 1110, 1242, 1502, 2015
MaeII ACGT 1 cut(s) 987
MaeIII GTNAC 4 cut(s) 1504, 1612, 2113, 2206
MalI GATC 7 cut(s) 159, 868, 932, 1227, 1830, 1925, 2147
MbiI CCGCTC 1 cut(s) 1688
MboI GATC 7 cut(s) 157, 866, 930, 1225, 1828, 1923, 2145
MfeI CAATTG 2 cut(s) 1425, 2295
MflI RGATCY 3 cut(s) 930, 1828, 2145
MhlI GDGCHC 3 cut(s) 453, 477, 1706
MlsI TGGCCA 1 cut(s) 648
MluCI AATT 5 cut(s) 297, 1212, 1425, 1906, 2295
MluNI TGGCCA 1 cut(s) 648
Mly113I GGCGCC 2 cut(s) 740, 2132
MlyI GAGTC 1 cut(s) 1183
MmeI TCCRAC 3 cut(s) 400, 577, 1008
Mox20I TGGCCA 1 cut(s) 648
MroNI GCCGGC 3 cut(s) 139, 388, 1274
MscI TGGCCA 1 cut(s) 648
MseI TTAA 2 cut(s) 1050, 1716
MslI CAYNNNNRTG 3 cut(s) 119, 437, 1659
Msp20I TGGCCA 1 cut(s) 648
MspA1I CMGCKG 4 cut(s) 809, 920, 950, 1358
MspR9I CCNGG 7 cut(s) 253, 1040, 1511, 1542, 1851, 2100, 2308
MunI CAATTG 2 cut(s) 1425, 2295
MvaI CCWGG 4 cut(s) 253, 1851, 2100, 2308
MvnI CGCG 6 cut(s) 83, 479, 563, 730, 761, 920
NaeI GCCGGC 3 cut(s) 141, 390, 1276
NarI GGCGCC 2 cut(s) 740, 2132
NciI CCSGG 3 cut(s) 1040, 1511, 1542
NdeII GATC 7 cut(s) 157, 866, 930, 1225, 1828, 1923, 2145
NgoMIV GCCGGC 3 cut(s) 139, 388, 1274
NheI GCTAGC 1 cut(s) 698
NmeAIII GCCGAG 1 cut(s) 289
NmuCI GTSAC 4 cut(s) 1504, 1612, 2113, 2206
NspI RCATGY 4 cut(s) 214, 1613, 1658, 1826
OliI CACNNNNGTG 1 cut(s) 437
PagI TCATGA 1 cut(s) 1728
PaqCI CACCTGC 1 cut(s) 85
PdiI GCCGGC 3 cut(s) 141, 390, 1276
PfeI GAWTC 2 cut(s) 176, 620
PflFI GACNNNGTC 1 cut(s) 836
PinAI ACCGGT 1 cut(s) 677
PleI GAGTC 1 cut(s) 1183
PluTI GGCGCC 2 cut(s) 743, 2135
PpsI GAGTC 1 cut(s) 1183
Psp124BI GAGCTC 1 cut(s) 453
Psp1406I AACGTT 1 cut(s) 987
Psp6I CCWGG 4 cut(s) 251, 1849, 2098, 2306
PspFI CCCAGC 2 cut(s) 1559, 1817
PspGI CCWGG 4 cut(s) 251, 1849, 2098, 2306
PspPI GGNCC 6 cut(s) 599, 962, 1246, 1493, 1580, 2265
PstI CTGCAG 1 cut(s) 1711
PsuI RGATCY 3 cut(s) 930, 1828, 2145
PsyI GACNNNGTC 1 cut(s) 836
PvuII CAGCTG 2 cut(s) 809, 950
RsaI GTAC 6 cut(s) 326, 486, 764, 1636, 2095, 2239
RsaNI GTAC 6 cut(s) 325, 485, 763, 1635, 2094, 2238
RseI CAYNNNNRTG 3 cut(s) 119, 437, 1659
SacI GAGCTC 1 cut(s) 453
SacII CCGCGG 1 cut(s) 921
SaqAI TTAA 2 cut(s) 1050, 1716
Sau3AI GATC 7 cut(s) 157, 866, 930, 1225, 1828, 1923, 2145
Sau96I GGNCC 6 cut(s) 599, 962, 1246, 1493, 1580, 2265
SchI GAGTC 1 cut(s) 1183
ScrFI CCNGG 7 cut(s) 253, 1040, 1511, 1542, 1851, 2100, 2308
SduI GDGCHC 3 cut(s) 453, 477, 1706
SfaNI GCATC 5 cut(s) 66, 1297, 1324, 1484, 1790
SfcI CTRYAG 5 cut(s) 591, 1005, 1350, 1416, 1707
SfoI GGCGCC 2 cut(s) 741, 2133
Sfr303I CCGCGG 1 cut(s) 921
SgrBI CCGCGG 1 cut(s) 921
SinI GGWCC 6 cut(s) 599, 962, 1246, 1493, 1580, 2265
SmiMI CAYNNNNRTG 3 cut(s) 119, 437, 1659
SmlI CTYRAG 1 cut(s) 1486
SmoI CTYRAG 1 cut(s) 1486
Sse9I AATT 5 cut(s) 297, 1212, 1425, 1906, 2295
SspDI GGCGCC 2 cut(s) 739, 2131
SspI AATATT 1 cut(s) 881
SspMI CTAG 6 cut(s) 699, 750, 1110, 1242, 1502, 2015
SstI GAGCTC 1 cut(s) 453
StyD4I CCNGG 7 cut(s) 251, 1038, 1509, 1540, 1849, 2098, 2306
StyI CCWWGG 2 cut(s) 995, 1241
TaaI ACNGT 9 cut(s) 595, 836, 873, 895, 1009, 1089, 2062, 2206, 2284
TaiI ACGT 1 cut(s) 990
TaqI TCGA 8 cut(s) 197, 575, 606, 623, 840, 1228, 1845, 1875
TaqII GACCGA 1 cut(s) 1510
TasI AATT 5 cut(s) 297, 1212, 1425, 1906, 2295
TauI GCSGC 6 cut(s) 482, 761, 920, 923, 1673, 1688
TfiI GAWTC 2 cut(s) 176, 620
Tru1I TTAA 2 cut(s) 1050, 1716
Tru9I TTAA 2 cut(s) 1050, 1716
TseFI GTSAC 4 cut(s) 1504, 1612, 2113, 2206
Tsp45I GTSAC 4 cut(s) 1504, 1612, 2113, 2206
TspDTI ATGAA 3 cut(s) 7, 17, 600
TspGWI ACGGA 5 cut(s) 181, 295, 666, 1329, 1457
Tth111I GACNNNGTC 1 cut(s) 836
VpaK11BI GGWCC 6 cut(s) 599, 962, 1246, 1493, 1580, 2265
XagI CCTNNNNNAGG 2 cut(s) 1108, 1679
XapI RAATTY 1 cut(s) 297
XceI RCATGY 4 cut(s) 214, 1613, 1658, 1826
XmaJI CCTAGG 1 cut(s) 1241
XspI CTAG 6 cut(s) 699, 750, 1110, 1242, 1502, 2015
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.