MD15G1072700.v1.1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001634
Physical Location & Seq
Forward (+)
4958834 .. 4961149
2316 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1072700.v1.1.491

Sequence Viewer

Length: 2316 bp
ATGAAGATATCCACTTTCATGCTTCTGCTAACAGTCATGCTTCTTGCTCTCTCTCACGTCTCCGCTATTGCGGAAGACGACGCGAGAGAGCAGCAGGTGAAAAAGACTTACATCATTCACATGGACAAGTCCGAAATGCCGGCAAGTTTTGAAGATGATCGTTTCCAATGGTATGATTCGTCTTTGAAATCCGTGTCGGATTCAGCCGACATGCTTTACGCTTACAAGACCATATTCCACGGCTTCGCCACCAGGCTAACCGCCGAGGAAGCTGGGCTGCTTGAGAAGCAGTCGGGAATTCTGTCCGTTCTGCCCGAAAGGAGGTACGAGCTGCATACAACACGGACGCCCGAGTTTCTTGGATTGGGAAAGAGTGAAGCCCTCTTACCAGCGACGGATAAAGCGAGTGATTTGATAGTCGGAGTGGTGGACACTGGCGTGTGGCCTGAGATCAAAAGCTACGATGACACAGGTCTCGCGGCGGTACCAAGTGGCTGGAAAGGGGTGTGTGAGGAGGGAACAAACTTCAACTCCTCGAGCTGCAACCGGAAACTCATTGGCGCGAGGTTTTTCTCAAAAGGGTATGAAGCTGCATTTGGACCAATCGATGTGAAAACAGAATCGAAATCACCACGAGATGACGATGGCCACGGAACTCACACCTCATCCACTGCAGCCGGCTCCGCAGTTTCAGGAGCTAGCCTCTTTGGTTATGCTTCGGGGACAGCGCGAGGGATGGCTCCACAAGCTCGAGTGGCCACATACAAGGCGTGCTGGCTTGGTGGGTGTTTCGGCTCTGATATCATAGCTGCAATGGAGAAGGCCATTGAAGACGGTGTCAATGTTTTATCTTTGTCTATCGGGGGACCACTAACTGATTATTACAAAGACACTGTTGCTATCGGAGCTTTCTCAGCCGTTGCACAGGGGATCCTTGTGTCGTGTTCAGCTGGTAATGGTGGGCCGGAAGCGGGGAGTTTGTCCAACGTTGCGCCTTGGATAACCACCGTCGGTGCTGGAACATTAGACAGGGATTTCCCGGCTTTTGTAAGCCTCGGAAGTGCAAAGAAATTCAGAGGTATATCGCTCTACGGGGGAACATCCTTATCTGGTGGATTACTTCCGCTTGTATATGCTCGCGATGCAAGTAACTCCTCCGATGGCGAGCTATGCGCTCCTGAAAGTTTGATTCCTGCAAAGGTTGCAGGGAAAATTGTGGTATGTGATCGAGGGGGAACGCCTAGGGCCCAAAAGAGTTTGGTGGTGAAACAGGCCGGTGGCTTAGGGATGATTTTGACGAACACTGATGATTACGGAGAAGAACTTGTTGCGGATGCATATCTACTGCCTGCAGCAGCAGTTGGTGCGATAGCTGGTGCTGCAATAAAAAGCTATATTGCCTCTGATTCTAATCCCACAGCCACAATTGCGCTTGGAGACACGGAGTTAGGTGTGCAGCCCTCGCCTGTGGTGGCATCATTCAGCTCCAGAGGACCAAATCTATTCACTCCCGAAATACTGAAACCAGACCTAATAGCACCGGGAGTAAATATCCTTGCTGGGTGGACTGGTGCCGTTGGACCAACCGGACTGGATGAAGACAAGAGGCGGGTCGACTTCAACATCATTTCAGGTACTTCCATGTCATGCCCCCATGTGAGTGGTTTGGCCGCCCTTGTCAAGGCTGCTCATCCAGAATGGAGCCCTGCAGCCATCAAGTCTGCACTCATGACCACATCCTACATAGCATACAAAACTGGAGAAACCATCATAGATGTAGCAACTGGAAATCCTGCAACGCCTTTCGATTATGGTGCTGGGCATGTGGATCCGGTGGCAGCCCTCGACCCTGGCCTTGTTTATGATAATGCGGTCGAGGACTACTTCAGCTTCCTCTGCGCCTTGAATTATAGCTCAAGTCAGATCAAACTAACCACTCACAAAGACTTCACTTGTGATACAAGCAAGAAATACAGCCTCAGAGATTTTAATTACCCGTCTTTTGCTGTTCCTCTAGAAACATCTTCAGGCAAAGGGGGCGGGACAGGTGCATCAACAACTGTAAAATACACAAGGACACTGACCAATGTGGGTACCCCAGGAACATACGAGGTCTCGGTATCTTCACAGGCGCCATCAGTGAAGATTTCGGTCAAACCACAATCCCTGACTTTCAGTCAAGCATACGAGAAGAAAACATATACCGTTACTTTTGTTGCAGGTTCTTCGCCATCTGGTACAAACAGCTTTGCTCGGTTGGCATGGTCCGATGGGAAACACACAGTAGGTAGTCCAATTGCTTTCAGCTGGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

772

Amino Acids

80.76

Weight (kDa)

5.39

Isoelectric Point (pI)

32.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 36 - 112 3.2e-16 Peptidase inhibitor I9
Peptidase_S8 PF00082 137 - 607 2.6e-45 Subtilase family
PA PF02225 375 - 462 3.1e-12 PA domain
fn3_6 PF17766 662 - 767 2.7e-25 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 85
Acc36I ACCTGC 2 cut(s) 85, 2210
Acc65I GGTACC 2 cut(s) 484, 2093
AccB1I GGYRCC 4 cut(s) 484, 1571, 2093, 2131
AccI GTMKAC 1 cut(s) 1614
AccII CGCG 5 cut(s) 83, 479, 563, 730, 1140
AclI AACGTT 1 cut(s) 987
AclWI GGATC 4 cut(s) 925, 938, 1823, 1836
AcoI YGGCCR 3 cut(s) 646, 756, 1668
AcsI RAATTY 2 cut(s) 297, 1070
AcuI CTGAAG 2 cut(s) 1870, 2010
AcyI GRCGYC 2 cut(s) 347, 2132
AfaI GTAC 5 cut(s) 326, 486, 1636, 2095, 2239
AfiI CCNNNNNNNGG 3 cut(s) 321, 971, 1681
AgsI TTSAA 6 cut(s) 152, 187, 529, 830, 1621, 1906
AhdI GACNNNNNGTC 1 cut(s) 2175
AjiI CACGTC 1 cut(s) 58
AjnI CCWGG 3 cut(s) 251, 1849, 2098
AjuI GAANNNNNNNTTGG 2 cut(s) 579, 611
AleI CACNNNNGTG 1 cut(s) 437
Alw26I GTCTC 4 cut(s) 64, 479, 1431, 2119
AlwI GGATC 4 cut(s) 925, 938, 1823, 1836
Ama87I CYCGRG 3 cut(s) 350, 535, 750
AoxI GGCC 9 cut(s) 443, 646, 756, 822, 962, 1245, 1272, 1668, 1852
ApaI GGGCCC 1 cut(s) 1249
ApoI RAATTY 2 cut(s) 297, 1070
Asp718I GGTACC 2 cut(s) 484, 2093
AspA2I CCTAGG 1 cut(s) 1241
AspLEI GCGC 7 cut(s) 563, 730, 994, 1175, 1432, 1901, 2134
AspS9I GGNCC 8 cut(s) 599, 866, 962, 1245, 1246, 1493, 1580, 2265
AsuC2I CCSGG 2 cut(s) 1040, 1542
AsuHPI GGTGA 3 cut(s) 109, 621, 1276
AsuNHI GCTAGC 1 cut(s) 698
AvaI CYCGRG 3 cut(s) 350, 535, 750
AvaII GGWCC 5 cut(s) 599, 866, 1493, 1580, 2265
AvrII CCTAGG 1 cut(s) 1241
BaeGI GKGCMC 1 cut(s) 1249
BalI TGGCCA 2 cut(s) 648, 758
BamHI GGATCC 2 cut(s) 930, 1828
BanI GGYRCC 4 cut(s) 484, 1571, 2093, 2131
BanII GRGCYC 2 cut(s) 1249, 1706
BarI GAAGNNNNNNTAC 2 cut(s) 369, 401
BauI CACGAG 1 cut(s) 633
BbsI GAAGAC 3 cut(s) 81, 837, 1605
BccI CCATC 8 cut(s) 638, 730, 1154, 1721, 1775, 2143, 2239, 2264
BceAI ACGGC 3 cut(s) 256, 902, 1559
BcgI CGANNNNNNTGC 4 cut(s) 1796, 1830, 2208, 2242
BciT130I CCWGG 3 cut(s) 253, 1851, 2100
BcnI CCSGG 2 cut(s) 1040, 1542
BcoDI GTCTC 4 cut(s) 64, 479, 1431, 2119
BfaI CTAG 3 cut(s) 699, 1242, 2015
BfmI CTRYAG 3 cut(s) 672, 1350, 1707
BfoI RGCGCY 1 cut(s) 2135
BfuAI ACCTGC 2 cut(s) 85, 2210
BlnI CCTAGG 1 cut(s) 1241
Bme1390I CCNGG 5 cut(s) 253, 1040, 1542, 1851, 2100
Bme18I GGWCC 5 cut(s) 599, 866, 1493, 1580, 2265
BmeRI GACNNNNNGTC 1 cut(s) 2175
BmeT110I CYCGRG 3 cut(s) 350, 535, 750
BmgBI CACGTC 1 cut(s) 58
BmgT120I GGNCC 8 cut(s) 599, 866, 962, 1245, 1246, 1493, 1580, 2265
BmrFI CCNGG 5 cut(s) 253, 1040, 1542, 1851, 2100
BmsI GCATC 4 cut(s) 1132, 1324, 1484, 2060
BmtI GCTAGC 1 cut(s) 702
BoxI GACNNNNGTC 1 cut(s) 471
BpiI GAAGAC 3 cut(s) 81, 837, 1605
BplI GAGNNNNNCTC 2 cut(s) 687, 719
BpmI CTGGAG 2 cut(s) 1471, 1779
Bpu10I CCTNAGC 1 cut(s) 1282
BpuEI CTTGAG 2 cut(s) 302, 1900
BpuMI CCSGG 2 cut(s) 1040, 1542
Bsa29I ATCGAT 1 cut(s) 606
BsaBI GATNNNNATC 2 cut(s) 1338, 1410
BsaHI GRCGYC 2 cut(s) 347, 2132
BsaI GGTCTC 2 cut(s) 479, 2119
BsaJI CCNNGG 8 cut(s) 238, 264, 649, 995, 1054, 1241, 1849, 2098
BsaWI WCCGGW 3 cut(s) 546, 1586, 1831
BsaXI ACNNNNNCTCC 2 cut(s) 515, 545
Bsc4I CCNNNNNNNGG 3 cut(s) 321, 971, 1681
Bse118I RCCGGY 3 cut(s) 139, 677, 1274
Bse1I ACTGG 5 cut(s) 439, 1573, 1596, 1762, 1789
Bse3DI GCAATG 1 cut(s) 819
Bse8I GATNNNNATC 2 cut(s) 1338, 1410
BseBI CCWGG 3 cut(s) 253, 1851, 2100
BseCI ATCGAT 1 cut(s) 606
BseDI CCNNGG 8 cut(s) 238, 264, 649, 995, 1054, 1241, 1849, 2098
BseGI GGATG 8 cut(s) 665, 741, 1100, 1293, 1339, 1600, 1690, 1736
BseJI GATNNNNATC 2 cut(s) 1338, 1410
BseLI CCNNNNNNNGG 3 cut(s) 321, 971, 1681
BseMI GCAATG 1 cut(s) 819
BseMII CTCAG 3 cut(s) 438, 927, 1993
BseNI ACTGG 5 cut(s) 439, 1573, 1596, 1762, 1789
BseRI GAGGAG 3 cut(s) 523, 527, 1144
BseSI GKGCMC 1 cut(s) 1249
BseYI CCCAGC 3 cut(s) 272, 1559, 1817
BsgI GTGCAG 2 cut(s) 1475, 1707
Bsh1236I CGCG 5 cut(s) 83, 479, 563, 730, 1140
Bsh1285I CGRYCG 1 cut(s) 1875
BshFI GGCC 9 cut(s) 445, 648, 758, 824, 964, 1247, 1274, 1670, 1854
BshNI GGYRCC 4 cut(s) 484, 1571, 2093, 2131
BshVI ATCGAT 1 cut(s) 606
BsiEI CGRYCG 1 cut(s) 1875
BsiHKCI CYCGRG 3 cut(s) 350, 535, 750
BsiSI CCGG 9 cut(s) 140, 547, 678, 965, 1040, 1275, 1541, 1587, 1832
BslFI GGGAC 3 cut(s) 736, 879, 2056
BslI CCNNNNNNNGG 3 cut(s) 321, 971, 1681
BsmAI GTCTC 4 cut(s) 64, 479, 1431, 2119
BsmBI CGTCTC 1 cut(s) 64
BsmFI GGGAC 3 cut(s) 736, 879, 2056
BsnI GGCC 9 cut(s) 445, 648, 758, 824, 964, 1247, 1274, 1670, 1854
Bso31I GGTCTC 2 cut(s) 479, 2119
BsoBI CYCGRG 3 cut(s) 350, 535, 750
Bsp120I GGGCCC 1 cut(s) 1245
Bsp1286I GDGCHC 2 cut(s) 1249, 1706
Bsp143I GATC 6 cut(s) 157, 450, 930, 1225, 1828, 1923
Bsp68I TCGCGA 1 cut(s) 1140
BspANI GGCC 9 cut(s) 445, 648, 758, 824, 964, 1247, 1274, 1670, 1854
BspCNI CTCAG 3 cut(s) 439, 926, 1992
BspDI ATCGAT 1 cut(s) 606
BspFNI CGCG 5 cut(s) 83, 479, 563, 730, 1140
BspHI TCATGA 1 cut(s) 1728
BspMAI CTGCAG 3 cut(s) 676, 1354, 1711
BspMI ACCTGC 2 cut(s) 85, 2210
BspOI GCTAGC 1 cut(s) 702
BspPI GGATC 4 cut(s) 925, 938, 1823, 1836
BspT107I GGYRCC 4 cut(s) 484, 1571, 2093, 2131
BspTNI GGTCTC 2 cut(s) 479, 2119
BsrDI GCAATG 1 cut(s) 819
BsrFI RCCGGY 3 cut(s) 139, 677, 1274
BsrI ACTGG 5 cut(s) 439, 1573, 1596, 1762, 1789
BssAI RCCGGY 3 cut(s) 139, 677, 1274
BssECI CCNNGG 8 cut(s) 238, 264, 649, 995, 1054, 1241, 1849, 2098
BssMI GATC 6 cut(s) 157, 450, 930, 1225, 1828, 1923
BssNI GRCGYC 2 cut(s) 347, 2132
BssSI CACGAG 1 cut(s) 633
BssT1I CCWWGG 2 cut(s) 995, 1241
Bst2BI CACGAG 1 cut(s) 633
Bst2UI CCWGG 3 cut(s) 253, 1851, 2100
Bst4CI ACNGT 7 cut(s) 34, 836, 895, 1009, 2062, 2206, 2284
BstACI GRCGYC 2 cut(s) 347, 2132
BstAPI GCANNNNNTGC 2 cut(s) 1202, 1364
BstC8I GCNNGC 8 cut(s) 141, 679, 700, 772, 776, 1138, 1166, 1350
BstDEI CTNAG 4 cut(s) 447, 913, 1282, 1979
BstDSI CCRYGG 2 cut(s) 238, 649
BstENI CCTNNNNNAGG 1 cut(s) 1679
BstF5I GGATG 8 cut(s) 665, 741, 1100, 1293, 1339, 1600, 1690, 1736
BstFNI CGCG 5 cut(s) 83, 479, 563, 730, 1140
BstH2I RGCGCY 1 cut(s) 2135
BstHHI GCGC 7 cut(s) 563, 730, 994, 1175, 1432, 1901, 2134
BstKTI GATC 6 cut(s) 160, 453, 933, 1228, 1831, 1926
BstMAI GTCTC 4 cut(s) 64, 479, 1431, 2119
BstMBI GATC 6 cut(s) 157, 450, 930, 1225, 1828, 1923
BstMCI CGRYCG 1 cut(s) 1875
BstNI CCWGG 3 cut(s) 253, 1851, 2100
BstNSI RCATGY 2 cut(s) 214, 1826
BstPAI GACNNNNGTC 1 cut(s) 471
BstSCI CCNGG 5 cut(s) 251, 1038, 1540, 1849, 2098
BstSFI CTRYAG 3 cut(s) 672, 1350, 1707
BstSLI GKGCMC 1 cut(s) 1249
BstUI CGCG 5 cut(s) 83, 479, 563, 730, 1140
BstV2I GAAGAC 3 cut(s) 81, 837, 1605
BstX2I RGATCY 2 cut(s) 930, 1828
BstXI CCANNNNNNTGG 2 cut(s) 495, 1661
BstYI RGATCY 2 cut(s) 930, 1828
Bsu15I ATCGAT 1 cut(s) 606
BsuRI GGCC 9 cut(s) 445, 648, 758, 824, 964, 1247, 1274, 1670, 1854
BsuTUI ATCGAT 1 cut(s) 606
BtgI CCRYGG 2 cut(s) 238, 649
BtgZI GCGATG 1 cut(s) 1155
BtrI CACGTC 1 cut(s) 58
BtsCI GGATG 8 cut(s) 665, 741, 1100, 1293, 1339, 1600, 1690, 1736
BtsI GCAGTG 1 cut(s) 669
BtsIMutI CAGTG 6 cut(s) 432, 669, 891, 1302, 2078, 2145
BtuMI TCGCGA 1 cut(s) 1140
BveI ACCTGC 2 cut(s) 85, 2210
Cac8I GCNNGC 8 cut(s) 141, 679, 700, 772, 776, 1138, 1166, 1350
CciI TCATGA 1 cut(s) 1728
CfoI GCGC 7 cut(s) 563, 730, 994, 1175, 1432, 1901, 2134
Cfr10I RCCGGY 3 cut(s) 139, 677, 1274
Cfr13I GGNCC 8 cut(s) 599, 866, 962, 1245, 1246, 1493, 1580, 2265
ClaI ATCGAT 1 cut(s) 606
CseI GACGC 2 cut(s) 89, 355
Csp6I GTAC 5 cut(s) 325, 485, 1635, 2094, 2238
CviQI GTAC 5 cut(s) 325, 485, 1635, 2094, 2238
DdeI CTNAG 4 cut(s) 447, 913, 1282, 1979
DinI GGCGCC 1 cut(s) 2133
DpnI GATC 6 cut(s) 159, 452, 932, 1227, 1830, 1925
DpnII GATC 6 cut(s) 157, 450, 930, 1225, 1828, 1923
DriI GACNNNNNGTC 1 cut(s) 2175
EaeI YGGCCR 3 cut(s) 646, 756, 1668
Eam1105I GACNNNNNGTC 1 cut(s) 2175
Eco130I CCWWGG 2 cut(s) 995, 1241
Eco24I GRGCYC 2 cut(s) 1249, 1706
Eco31I GGTCTC 2 cut(s) 479, 2119
Eco32I GATATC 2 cut(s) 9, 802
Eco47I GGWCC 5 cut(s) 599, 866, 1493, 1580, 2265
Eco57I CTGAAG 2 cut(s) 1870, 2010
Eco88I CYCGRG 3 cut(s) 350, 535, 750
EcoNI CCTNNNNNAGG 1 cut(s) 1679
EcoO109I RGGNCCY 1 cut(s) 1245
EcoRI GAATTC 1 cut(s) 297
EcoRII CCWGG 3 cut(s) 251, 1849, 2098
EcoRV GATATC 2 cut(s) 9, 802
EcoT14I CCWWGG 2 cut(s) 995, 1241
EcoT22I ATGCAT 1 cut(s) 1339
EcoT38I GRGCYC 2 cut(s) 1249, 1706
EgeI GGCGCC 1 cut(s) 2133
EheI GGCGCC 1 cut(s) 2133
ErhI CCWWGG 2 cut(s) 995, 1241
Esp3I CGTCTC 1 cut(s) 64
FaqI GGGAC 3 cut(s) 736, 879, 2056
FauI CCCGC 3 cut(s) 964, 1602, 2033
FblI GTMKAC 1 cut(s) 1614
FokI GGATG 8 cut(s) 652, 748, 1087, 1300, 1346, 1607, 1677, 1723
FriOI GRGCYC 2 cut(s) 1249, 1706
FspBI CTAG 3 cut(s) 699, 1242, 2015
GlaI GCGC 7 cut(s) 562, 729, 993, 1174, 1431, 1900, 2133
GsaI CCCAGC 3 cut(s) 276, 1563, 1821
GsuI CTGGAG 2 cut(s) 1471, 1779
HaeII RGCGCY 1 cut(s) 2135
HaeIII GGCC 9 cut(s) 445, 648, 758, 824, 964, 1247, 1274, 1670, 1854
HapII CCGG 9 cut(s) 140, 547, 678, 965, 1040, 1275, 1541, 1587, 1832
HgaI GACGC 2 cut(s) 89, 355
HhaI GCGC 7 cut(s) 563, 730, 994, 1175, 1432, 1901, 2134
Hin1I GRCGYC 2 cut(s) 347, 2132
Hin6I GCGC 7 cut(s) 561, 728, 992, 1173, 1430, 1899, 2132
HinP1I GCGC 7 cut(s) 561, 728, 992, 1173, 1430, 1899, 2132
HincII GTYRAC 1 cut(s) 1615
HindII GTYRAC 1 cut(s) 1615
HinfI GANTC 5 cut(s) 176, 200, 620, 1189, 1406
HpaII CCGG 9 cut(s) 140, 547, 678, 965, 1040, 1275, 1541, 1587, 1832
HphI GGTGA 3 cut(s) 109, 621, 1276
Hpy166II GTNNAC 3 cut(s) 430, 1566, 1615
Hpy188III TCNNGA 9 cut(s) 294, 693, 1139, 1178, 1488, 1511, 1694, 1729, 2015
Hpy8I GTNNAC 3 cut(s) 430, 1566, 1615
Hpy99I CGWCG 3 cut(s) 83, 397, 1013
HpyAV CCTTC 1 cut(s) 814
HpyCH4III ACNGT 7 cut(s) 34, 836, 895, 1009, 2062, 2206, 2284
HpyCH4IV ACGT 2 cut(s) 57, 987
HpyF3I CTNAG 4 cut(s) 447, 913, 1282, 1979
HpySE526I ACGT 2 cut(s) 57, 987
Hsp92I GRCGYC 2 cut(s) 347, 2132
HspAI GCGC 7 cut(s) 561, 728, 992, 1173, 1430, 1899, 2132
KasI GGCGCC 1 cut(s) 2131
KpnI GGTACC 2 cut(s) 488, 2097
KroI GCCGGC 2 cut(s) 139, 677
KroNI GCCGGC 2 cut(s) 141, 679
Kzo9I GATC 6 cut(s) 157, 450, 930, 1225, 1828, 1923
LmnI GCTCC 7 cut(s) 686, 695, 745, 905, 1180, 1490, 1701
LweI GCATC 4 cut(s) 1132, 1324, 1484, 2060
MaeI CTAG 3 cut(s) 699, 1242, 2015
MaeII ACGT 2 cut(s) 57, 987
MaeIII GTNAC 2 cut(s) 1148, 2206
MalI GATC 6 cut(s) 159, 452, 932, 1227, 1830, 1925
MboI GATC 6 cut(s) 157, 450, 930, 1225, 1828, 1923
MfeI CAATTG 2 cut(s) 1425, 2295
MflI RGATCY 2 cut(s) 930, 1828
MhlI GDGCHC 2 cut(s) 1249, 1706
MlsI TGGCCA 2 cut(s) 648, 758
MluCI AATT 7 cut(s) 297, 1070, 1212, 1425, 1906, 1990, 2295
MluNI TGGCCA 2 cut(s) 648, 758
Mly113I GGCGCC 1 cut(s) 2132
MmeI TCCRAC 4 cut(s) 177, 400, 1008, 1558
Mox20I TGGCCA 2 cut(s) 648, 758
Mph1103I ATGCAT 1 cut(s) 1339
MroNI GCCGGC 2 cut(s) 139, 677
MscI TGGCCA 2 cut(s) 648, 758
MseI TTAA 1 cut(s) 1989
MslI CAYNNNNRTG 4 cut(s) 17, 119, 437, 1659
Msp20I TGGCCA 2 cut(s) 648, 758
MspA1I CMGCKG 2 cut(s) 950, 2307
MspI CCGG 9 cut(s) 140, 547, 678, 965, 1040, 1275, 1541, 1587, 1832
MspR9I CCNGG 5 cut(s) 253, 1040, 1542, 1851, 2100
MunI CAATTG 2 cut(s) 1425, 2295
MvaI CCWGG 3 cut(s) 253, 1851, 2100
MvnI CGCG 5 cut(s) 83, 479, 563, 730, 1140
NaeI GCCGGC 2 cut(s) 141, 679
NarI GGCGCC 1 cut(s) 2132
NciI CCSGG 2 cut(s) 1040, 1542
NdeII GATC 6 cut(s) 157, 450, 930, 1225, 1828, 1923
NgoMIV GCCGGC 2 cut(s) 139, 677
NheI GCTAGC 1 cut(s) 698
NmeAIII GCCGAG 1 cut(s) 289
NruI TCGCGA 1 cut(s) 1140
NsiI ATGCAT 1 cut(s) 1339
NspI RCATGY 2 cut(s) 214, 1826
OliI CACNNNNGTG 1 cut(s) 437
PaeR7I CTCGAG 2 cut(s) 535, 750
PagI TCATGA 1 cut(s) 1728
PaqCI CACCTGC 1 cut(s) 85
PcsI WCGNNNNNNNCGW 1 cut(s) 401
PdiI GCCGGC 2 cut(s) 141, 679
PfeI GAWTC 5 cut(s) 176, 200, 620, 1189, 1406
PflFI GACNNNGTC 1 cut(s) 836
PluTI GGCGCC 1 cut(s) 2135
PshAI GACNNNNGTC 1 cut(s) 471
Psp1406I AACGTT 1 cut(s) 987
Psp6I CCWGG 3 cut(s) 251, 1849, 2098
PspFI CCCAGC 3 cut(s) 272, 1559, 1817
PspGI CCWGG 3 cut(s) 251, 1849, 2098
PspOMI GGGCCC 1 cut(s) 1245
PspPI GGNCC 8 cut(s) 599, 866, 962, 1245, 1246, 1493, 1580, 2265
PspXI VCTCGAGB 2 cut(s) 535, 750
PstI CTGCAG 3 cut(s) 676, 1354, 1711
PsuI RGATCY 2 cut(s) 930, 1828
PsyI GACNNNGTC 1 cut(s) 836
PvuII CAGCTG 2 cut(s) 950, 2307
RruI TCGCGA 1 cut(s) 1140
RsaI GTAC 5 cut(s) 326, 486, 1636, 2095, 2239
RsaNI GTAC 5 cut(s) 325, 485, 1635, 2094, 2238
RseI CAYNNNNRTG 4 cut(s) 17, 119, 437, 1659
SalI GTCGAC 1 cut(s) 1613
SaqAI TTAA 1 cut(s) 1989
Sau3AI GATC 6 cut(s) 157, 450, 930, 1225, 1828, 1923
Sau96I GGNCC 8 cut(s) 599, 866, 962, 1245, 1246, 1493, 1580, 2265
ScrFI CCNGG 5 cut(s) 253, 1040, 1542, 1851, 2100
SduI GDGCHC 2 cut(s) 1249, 1706
SfaNI GCATC 4 cut(s) 1132, 1324, 1484, 2060
SfcI CTRYAG 3 cut(s) 672, 1350, 1707
SfoI GGCGCC 1 cut(s) 2133
Sfr274I CTCGAG 2 cut(s) 535, 750
SinI GGWCC 5 cut(s) 599, 866, 1493, 1580, 2265
SlaI CTCGAG 2 cut(s) 535, 750
SmiMI CAYNNNNRTG 4 cut(s) 17, 119, 437, 1659
SmlI CTYRAG 4 cut(s) 281, 535, 750, 1915
SmoI CTYRAG 4 cut(s) 281, 535, 750, 1915
Sse9I AATT 7 cut(s) 297, 1070, 1212, 1425, 1906, 1990, 2295
SspDI GGCGCC 1 cut(s) 2131
SspMI CTAG 3 cut(s) 699, 1242, 2015
StyD4I CCNGG 5 cut(s) 251, 1038, 1540, 1849, 2098
StyI CCWWGG 2 cut(s) 995, 1241
TaaI ACNGT 7 cut(s) 34, 836, 895, 1009, 2062, 2206, 2284
TaiI ACGT 2 cut(s) 60, 990
TaqI TCGA 9 cut(s) 536, 606, 623, 751, 1228, 1614, 1806, 1845, 1875
TaqII GACCGA 1 cut(s) 2140
TasI AATT 7 cut(s) 297, 1070, 1212, 1425, 1906, 1990, 2295
TauI GCSGC 2 cut(s) 482, 1673
TfiI GAWTC 5 cut(s) 176, 200, 620, 1189, 1406
Tru1I TTAA 1 cut(s) 1989
Tru9I TTAA 1 cut(s) 1989
TscAI CASTG 6 cut(s) 439, 676, 898, 1309, 2085, 2145
TspDTI ATGAA 4 cut(s) 7, 17, 600, 1611
TspGWI ACGGA 7 cut(s) 181, 295, 358, 410, 666, 1329, 1457
TspRI CASTG 6 cut(s) 439, 676, 898, 1309, 2085, 2145
Tth111I GACNNNGTC 1 cut(s) 836
VpaK11BI GGWCC 5 cut(s) 599, 866, 1493, 1580, 2265
XagI CCTNNNNNAGG 1 cut(s) 1679
XapI RAATTY 2 cut(s) 297, 1070
XbaI TCTAGA 1 cut(s) 2014
XceI RCATGY 2 cut(s) 214, 1826
XhoI CTCGAG 2 cut(s) 535, 750
XmaJI CCTAGG 1 cut(s) 1241
XmiI GTMKAC 1 cut(s) 1614
XspI CTAG 3 cut(s) 699, 1242, 2015
Zsp2I ATGCAT 1 cut(s) 1339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.