Rh6DG484600

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0001634
Physical Location & Seq
Reverse (-)
64700692 .. 64705044
4353 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG484600.1

Sequence Viewer

Length: 2313 bp
ATGAAGAAGGCATCGAAATCCGTGCTTCTGGTAACGCTTATGGTTCTGGTTATCACTCACAACTGTGAGGTGGTAGCAGAAGAGAGTGAGCATGTGAAAAAGACGTACATCATTCACATGGACAAGTCCCAAATGCCCGCGAGTTTCGACGACGACCATGTCAACTGGTACGGCTCGTCGTTGAAAGCGGTCTCCGATTCAGCTGACATGCTTTACACGTACAAGACTGTCATCCATGGCTACTCAACAAGGCTAACTGCCGAGGAAGCTGAGCAGCTCGAAAAGCAGCCAGGAATTCTCTCTGTCCTGCCGGAAAGGAGGTACGAGCTTCACACAACTAGGACTCCGGAGTTTCTTGGACTAGGCAAAAGCGAAGCTGTGTTACCGGCTTCGGATAAGATGGGTGAGGTGATCATCGGAGTAGTGGACACCGGCGTGTGGCCGGAGCTCAAAAGCTACGACGACACTGGTCTTGGACCAGTGCCGAGTGGCTGGAAAGGGTCGTGCCAGCAGGGAAAGACATTCAACTCTTCGAGCTGCAACAAAAAGCTGATTGGTGCTGCGTTTTTCTCAAACGGGTACGAAGCAGCGTTTGGACCTGTTGATGAAAACGTAGAATCAAAGTCACCGAGAGATGACGATGGCCATGGAACTCACACCTCAACAACTGCGGCGGGGTCTGTTGTTACAGGAGCTAGCTTCCTTGGTTATGCTTCAGGGACAGCTAGGGGTATGGCGCCGCAAGCTCGAGTGGCCACATACAAGGCTTGCTGGCTTGGAGGGTGTTTCGGTTCTGATATCATAGCTGCAATGGAGAAGGCGGTTGAAGATGGGGTTGATGTTTTGTCTTTGTCTATTGGTGGATCGCAGTCTGATTATTATAGAGACACGGTTGCCATTGGAGCTTTCGCTGCCGCGGCGCAGGGTATTCTAGTATCGTGTTCGGCTGGAAATGGTGGACCGGAAGCGGGAAGCTTGTCCAATGTTGCGCCTTGGATAACTACTGTGGGCGCTGGAACATTGGACAGGGACTTCCCGGCTTATGTCAGCCTTGGAAATGCAAAGAAATTTAGAGGTATATCTCTATACAGGGGAACGGCCTTGTCTAGTGGATTGATTCCACTTGTTGATGCTCGCAATGCGAGCAGTTCGAGTGGTGATCTGTGCAGTCCCGGCAGTCTGATTCCGGCAAAGGTTGCCGGGAAGATTGTGGTATGTGACCGAGGGGGAACTCCAAGAGTTCAAAAGAGTCTGGTGGTGAAAAAGGCTGGTGGTGTGGGAATGATTTTGACCAACACCGAAACATATGGTGAAGAGCTTGTTGCTGATGCATATTTCTTGCCTACAGCAGCAGTTGGCCAGAAAGCTGGTGACGCAATAAAGAGCTATATTGCATCCGATTCTAATCCAACAGCAACAATTTCTCTTGGAGAAACGGAGTTAGGCGTTCAACCTTCACCGGTAGTGGCTGCGTTTAGCTCTAGAGGACCGAATTTTGTCACTCCGGAAGTACTCAAACCAGACCTGATTGCACCAGGAATGAACATCCTAGCTGGGTGGACTGGCGCAGTTGGACCAACTGGACTTCCTGAAGACAAGAGGAGGGTGGGCTTCAACATCATTTCAGGTACATCCATGTCGTGTCCACACGTGAGTGGGCTCGCGGCCCTTGTCAAGGCGTCTCACCCAGACTGGAGCCCCGCGGCCATTAAGTCTGCCCTCATGACCACAGCCTATTCTAACTACAAAAGTACTGGAGAAACAATCGAAGATGTTTCTACTGGAAGTGCTGCAACACCTTTCGATTATGGGGCTGGGCATGTGGATCCAGTGGCAGCTCTTGACCCTGGCCTTGTCTACGATCTTGCTGTTGAAGACTACCTAAGTTTCCTCTGTGCCTTGAATTATTCCCCTTCTCAGATCAAGCAAACCACTAAGAAGGACTTCACCTGTGACTCAAGCAAGAAATACAGCCTCGGAGATTTGAATTACCCGTCCTTTTCTCTTGCTCTAGATACATCTTCAGGCCAAGGGGGTGGTACTGGTGCATCAACCACTTTAAAGTACACAAGGACTCTGACCAATGTGGGTGCCCCGGCAACATACAAGGTCTCGATGTCTTCACTGACCAGATCAGTGAAGATGTCCGTAGAACCAGAATCACTGAGTTTCAGTGATGCGTATGAGAAGAAGACATACACTGTGACTTTTGTTGCTAGCTCTTTGCCATCCGGTACAGCCAGCTTTGGTCGCTTGGAATGGTCTGATGGGAAACACATAGTAGGTAGTCCTATTGCTTACACCTGGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

770

Amino Acids

80.6

Weight (kDa)

5.59

Isoelectric Point (pI)

34.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 35 - 111 3e-19 Peptidase inhibitor I9
Peptidase_S8 PF00082 136 - 606 3.7e-45 Subtilase family
PA PF02225 375 - 460 9.2e-14 PA domain
fn3_6 PF17766 661 - 766 1.8e-24 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 158
AccB1I GGYRCC 2 cut(s) 736, 2090
AccI GTMKAC 1 cut(s) 1857
AccII CGCG 4 cut(s) 140, 917, 1664, 1703
AccIII TCCGGA 2 cut(s) 346, 1504
AclWI GGATC 3 cut(s) 871, 1820, 1833
AcoI YGGCCR 5 cut(s) 440, 643, 753, 1357, 1704
AcsI RAATTY 3 cut(s) 294, 1067, 1492
AcuI CTGAAG 3 cut(s) 699, 1611, 2007
AcvI CACGTG 1 cut(s) 1651
AcyI GRCGYC 2 cut(s) 737, 1679
AfiI CCNNNNNNNGG 3 cut(s) 438, 968, 1675
AflIII ACRYGT 2 cut(s) 216, 1648
AgeI ACCGGT 1 cut(s) 1459
AgsI TTSAA 9 cut(s) 184, 526, 827, 1244, 1451, 1615, 1874, 1903, 1987
AjnI CCWGG 4 cut(s) 289, 1534, 1846, 2303
AjuI GAANNNNNNNTTGG 2 cut(s) 576, 608
AleI CACNNNNGTG 3 cut(s) 63, 434, 1653
Alw21I GWGCWC 1 cut(s) 450
Alw26I GTCTC 4 cut(s) 196, 879, 1686, 2116
AlwI GGATC 3 cut(s) 871, 1820, 1833
Ama87I CYCGRG 1 cut(s) 747
Aor13HI TCCGGA 2 cut(s) 346, 1504
AoxI GGCC 9 cut(s) 440, 643, 753, 1098, 1357, 1665, 1704, 1849, 2026
ApoI RAATTY 3 cut(s) 294, 1067, 1492
AsiGI ACCGGT 1 cut(s) 1459
Asp700I GAANNNNTTC 1 cut(s) 1943
AspLEI GCGC 5 cut(s) 739, 922, 991, 1013, 1568
AspS9I GGNCC 6 cut(s) 476, 596, 959, 1487, 1574, 1666
AsuC2I CCSGG 4 cut(s) 1037, 1173, 1201, 2096
AsuNHI GCTAGC 2 cut(s) 695, 2216
AvaI CYCGRG 1 cut(s) 747
AvaII GGWCC 5 cut(s) 476, 596, 959, 1487, 1574
BaeGI GKGCMC 1 cut(s) 2095
BalI TGGCCA 3 cut(s) 645, 755, 1359
BamHI GGATCC 1 cut(s) 1825
BanI GGYRCC 2 cut(s) 736, 2090
BanII GRGCYC 3 cut(s) 450, 1662, 1700
BarI GAAGNNNNNNTAC 4 cut(s) 366, 398, 2180, 2212
BbrPI CACGTG 1 cut(s) 1651
BbsI GAAGAC 4 cut(s) 1599, 1881, 2112, 2198
Bbv12I GWGCWC 1 cut(s) 450
BccI CCATC 5 cut(s) 394, 635, 824, 2236, 2261
BceAI ACGGC 2 cut(s) 187, 1113
BcgI CGANNNNNNTGC 2 cut(s) 4, 38
BciT130I CCWGG 4 cut(s) 291, 1536, 1848, 2305
BclI TGATCA 1 cut(s) 411
BcnI CCSGG 4 cut(s) 1037, 1173, 1201, 2096
BcoDI GTCTC 4 cut(s) 196, 879, 1686, 2116
BfmI CTRYAG 1 cut(s) 1344
BfoI RGCGCY 2 cut(s) 740, 1014
BlpI GCTNAGC 1 cut(s) 270
BmcAI AGTACT 2 cut(s) 1512, 1753
Bme1390I CCNGG 8 cut(s) 291, 1037, 1173, 1201, 1536, 1848, 2096, 2305
Bme18I GGWCC 5 cut(s) 476, 596, 959, 1487, 1574
BmeT110I CYCGRG 1 cut(s) 747
BmgT120I GGNCC 6 cut(s) 476, 596, 959, 1487, 1574, 1666
BmiI GGNNCC 4 cut(s) 738, 1697, 1827, 2092
BmrFI CCNGG 8 cut(s) 291, 1037, 1173, 1201, 1536, 1848, 2096, 2305
BmsI GCATC 6 cut(s) 20, 1120, 1318, 1403, 2057, 2167
BmtI GCTAGC 2 cut(s) 699, 2220
BoxI GACNNNNGTC 1 cut(s) 468
BpiI GAAGAC 4 cut(s) 1599, 1881, 2112, 2198
BpmI CTGGAG 2 cut(s) 1714, 1776
Bpu1102I GCTNAGC 1 cut(s) 270
BpuEI CTTGAG 1 cut(s) 1942
BpuMI CCSGG 4 cut(s) 1037, 1173, 1201, 2096
BsaAI YACGTR 2 cut(s) 219, 1651
BsaBI GATNNNNATC 1 cut(s) 1404
BsaHI GRCGYC 2 cut(s) 737, 1679
BsaI GGTCTC 2 cut(s) 196, 2116
BsaWI WCCGGW 5 cut(s) 346, 961, 1459, 1504, 2231
BsaXI ACNNNNNCTCC 2 cut(s) 328, 358
Bsc4I CCNNNNNNNGG 3 cut(s) 438, 968, 1675
Bse118I RCCGGY 3 cut(s) 385, 431, 1459
Bse3DI GCAATG 2 cut(s) 816, 1144
Bse8I GATNNNNATC 1 cut(s) 1404
BseAI TCCGGA 2 cut(s) 346, 1504
BseBI CCWGG 4 cut(s) 291, 1536, 1848, 2305
BseGI GGATG 5 cut(s) 231, 1394, 1545, 1631, 2228
BseJI GATNNNNATC 1 cut(s) 1404
BseLI CCNNNNNNNGG 3 cut(s) 438, 968, 1675
BseMI GCAATG 2 cut(s) 816, 1144
BseMII CTCAG 3 cut(s) 261, 1931, 2156
BseRI GAGGAG 1 cut(s) 1615
BseSI GKGCMC 1 cut(s) 2095
BseYI CCCAGC 2 cut(s) 1553, 1814
BsgI GTGCAG 1 cut(s) 1186
Bsh1236I CGCG 4 cut(s) 140, 917, 1664, 1703
BshFI GGCC 9 cut(s) 442, 645, 755, 1100, 1359, 1667, 1706, 1851, 2028
BshNI GGYRCC 2 cut(s) 736, 2090
BshTI ACCGGT 1 cut(s) 1459
BsiHKAI GWGCWC 1 cut(s) 450
BsiHKCI CYCGRG 1 cut(s) 747
BslFI GGGAC 4 cut(s) 112, 733, 1043, 1155
BslI CCNNNNNNNGG 3 cut(s) 438, 968, 1675
BsmAI GTCTC 4 cut(s) 196, 879, 1686, 2116
BsmBI CGTCTC 1 cut(s) 1686
BsmFI GGGAC 4 cut(s) 112, 733, 1043, 1155
BsnI GGCC 9 cut(s) 442, 645, 755, 1100, 1359, 1667, 1706, 1851, 2028
Bso31I GGTCTC 2 cut(s) 196, 2116
BsoBI CYCGRG 1 cut(s) 747
Bsp1286I GDGCHC 4 cut(s) 450, 1662, 1700, 2095
Bsp13I TCCGGA 2 cut(s) 346, 1504
Bsp143I GATC 7 cut(s) 411, 863, 1159, 1825, 1861, 1920, 2132
Bsp1720I GCTNAGC 1 cut(s) 270
Bsp19I CCATGG 2 cut(s) 235, 646
BspANI GGCC 9 cut(s) 442, 645, 755, 1100, 1359, 1667, 1706, 1851, 2028
BspCNI CTCAG 3 cut(s) 262, 1930, 2157
BspEI TCCGGA 2 cut(s) 346, 1504
BspFNI CGCG 4 cut(s) 140, 917, 1664, 1703
BspHI TCATGA 1 cut(s) 1722
BspLI GGNNCC 4 cut(s) 738, 1697, 1827, 2092
BspOI GCTAGC 2 cut(s) 699, 2220
BspPI GGATC 3 cut(s) 871, 1820, 1833
BspQI GCTCTTC 1 cut(s) 1308
BspT107I GGYRCC 2 cut(s) 736, 2090
BspTNI GGTCTC 2 cut(s) 196, 2116
BsrDI GCAATG 2 cut(s) 816, 1144
BsrFI RCCGGY 3 cut(s) 385, 431, 1459
BssAI RCCGGY 3 cut(s) 385, 431, 1459
BssMI GATC 7 cut(s) 411, 863, 1159, 1825, 1861, 1920, 2132
BssNI GRCGYC 2 cut(s) 737, 1679
BssT1I CCWWGG 6 cut(s) 235, 646, 703, 992, 1051, 2029
Bst2UI CCWGG 4 cut(s) 291, 1536, 1848, 2305
Bst4CI ACNGT 5 cut(s) 65, 229, 892, 1006, 2203
Bst6I CTCTTC 3 cut(s) 75, 535, 1308
BstACI GRCGYC 2 cut(s) 737, 1679
BstAPI GCANNNNNTGC 1 cut(s) 1196
BstBAI YACGTR 2 cut(s) 219, 1651
BstDEI CTNAG 5 cut(s) 270, 1883, 1917, 1935, 2165
BstDSI CCRYGG 4 cut(s) 235, 646, 915, 1701
BstENI CCTNNNNNAGG 1 cut(s) 1673
BstF5I GGATG 5 cut(s) 231, 1394, 1545, 1631, 2228
BstFNI CGCG 4 cut(s) 140, 917, 1664, 1703
BstH2I RGCGCY 2 cut(s) 740, 1014
BstHHI GCGC 5 cut(s) 739, 922, 991, 1013, 1568
BstKTI GATC 7 cut(s) 414, 866, 1162, 1828, 1864, 1923, 2135
BstMAI GTCTC 4 cut(s) 196, 879, 1686, 2116
BstMBI GATC 7 cut(s) 411, 863, 1159, 1825, 1861, 1920, 2132
BstNI CCWGG 4 cut(s) 291, 1536, 1848, 2305
BstNSI RCATGY 3 cut(s) 95, 211, 1823
BstPAI GACNNNNGTC 1 cut(s) 468
BstSCI CCNGG 8 cut(s) 289, 1035, 1171, 1199, 1534, 1846, 2094, 2303
BstSFI CTRYAG 1 cut(s) 1344
BstSLI GKGCMC 1 cut(s) 2095
BstUI CGCG 4 cut(s) 140, 917, 1664, 1703
BstV2I GAAGAC 4 cut(s) 1599, 1881, 2112, 2198
BstX2I RGATCY 1 cut(s) 1825
BstXI CCANNNNNNTGG 2 cut(s) 1367, 2036
BstYI RGATCY 1 cut(s) 1825
BsuRI GGCC 9 cut(s) 442, 645, 755, 1100, 1359, 1667, 1706, 1851, 2028
BtgI CCRYGG 4 cut(s) 235, 646, 915, 1701
BtsCI GGATG 5 cut(s) 231, 1394, 1545, 1631, 2228
BtsIMutI CAGTG 8 cut(s) 465, 486, 1836, 2123, 2142, 2162, 2179, 2199
CciI TCATGA 1 cut(s) 1722
CfoI GCGC 5 cut(s) 739, 922, 991, 1013, 1568
Cfr10I RCCGGY 3 cut(s) 385, 431, 1459
Cfr13I GGNCC 6 cut(s) 476, 596, 959, 1487, 1574, 1666
Cfr42I CCGCGG 2 cut(s) 918, 1704
CseI GACGC 2 cut(s) 1382, 1668
CspAI ACCGGT 1 cut(s) 1459
CviAII CATG 9 cut(s) 92, 118, 158, 208, 236, 647, 1636, 1723, 1820
DdeI CTNAG 5 cut(s) 270, 1883, 1917, 1935, 2165
DinI GGCGCC 1 cut(s) 738
DpnI GATC 7 cut(s) 413, 865, 1161, 1827, 1863, 1922, 2134
DpnII GATC 7 cut(s) 411, 863, 1159, 1825, 1861, 1920, 2132
DraI TTTAAA 1 cut(s) 2061
DrdI GACNNNNNNGTC 1 cut(s) 158
DseDI GACNNNNNNGTC 1 cut(s) 158
EaeI YGGCCR 5 cut(s) 440, 643, 753, 1357, 1704
Eam1104I CTCTTC 3 cut(s) 75, 535, 1308
EarI CTCTTC 3 cut(s) 75, 535, 1308
Ecl136II GAGCTC 1 cut(s) 448
Eco130I CCWWGG 6 cut(s) 235, 646, 703, 992, 1051, 2029
Eco24I GRGCYC 3 cut(s) 450, 1662, 1700
Eco31I GGTCTC 2 cut(s) 196, 2116
Eco32I GATATC 1 cut(s) 799
Eco47I GGWCC 5 cut(s) 476, 596, 959, 1487, 1574
Eco53kI GAGCTC 1 cut(s) 448
Eco57I CTGAAG 3 cut(s) 699, 1611, 2007
Eco72I CACGTG 1 cut(s) 1651
Eco88I CYCGRG 1 cut(s) 747
EcoICRI GAGCTC 1 cut(s) 448
EcoNI CCTNNNNNAGG 1 cut(s) 1673
EcoRI GAATTC 1 cut(s) 294
EcoRII CCWGG 4 cut(s) 289, 1534, 1846, 2303
EcoRV GATATC 1 cut(s) 799
EcoT14I CCWWGG 6 cut(s) 235, 646, 703, 992, 1051, 2029
EcoT22I ATGCAT 1 cut(s) 1333
EcoT38I GRGCYC 3 cut(s) 450, 1662, 1700
EgeI GGCGCC 1 cut(s) 738
EheI GGCGCC 1 cut(s) 738
ErhI CCWWGG 6 cut(s) 235, 646, 703, 992, 1051, 2029
Esp3I CGTCTC 1 cut(s) 1686
FaeI CATG 9 cut(s) 95, 121, 161, 211, 239, 650, 1639, 1726, 1823
FalI AAGNNNNNCTT 2 cut(s) 1928, 1960
FaqI GGGAC 4 cut(s) 112, 733, 1043, 1155
FatI CATG 9 cut(s) 91, 117, 157, 207, 235, 646, 1635, 1722, 1819
FauI CCCGC 4 cut(s) 145, 667, 961, 1708
FauNDI CATATG 1 cut(s) 1306
FbaI TGATCA 1 cut(s) 411
FblI GTMKAC 1 cut(s) 1857
FokI GGATG 5 cut(s) 218, 1381, 1532, 1618, 2215
FriOI GRGCYC 3 cut(s) 450, 1662, 1700
GlaI GCGC 5 cut(s) 738, 921, 990, 1012, 1567
GsaI CCCAGC 2 cut(s) 1557, 1818
GsuI CTGGAG 2 cut(s) 1714, 1776
HaeII RGCGCY 2 cut(s) 740, 1014
HaeIII GGCC 9 cut(s) 442, 645, 755, 1100, 1359, 1667, 1706, 1851, 2028
HgaI GACGC 2 cut(s) 1382, 1668
HhaI GCGC 5 cut(s) 739, 922, 991, 1013, 1568
Hin1I GRCGYC 2 cut(s) 737, 1679
Hin1II CATG 9 cut(s) 95, 121, 161, 211, 239, 650, 1639, 1726, 1823
Hin6I GCGC 5 cut(s) 737, 920, 989, 1011, 1566
HinP1I GCGC 5 cut(s) 737, 920, 989, 1011, 1566
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HindIII AAGCTT 1 cut(s) 973
Hpy166II GTNNAC 7 cut(s) 163, 427, 959, 1560, 1646, 1858, 2067
Hpy188III TCNNGA 8 cut(s) 347, 1482, 1505, 1589, 1723, 1841, 2012, 2113
Hpy8I GTNNAC 7 cut(s) 163, 427, 959, 1560, 1646, 1858, 2067
Hpy99I CGWCG 4 cut(s) 152, 155, 181, 464
HpyAV CCTTC 4 cut(s) 811, 1464, 1923, 1933
HpyCH4III ACNGT 5 cut(s) 65, 229, 892, 1006, 2203
HpyCH4IV ACGT 4 cut(s) 104, 218, 612, 1650
HpyCH4V TGCA 9 cut(s) 540, 809, 1061, 1167, 1331, 1394, 1532, 1793, 2048
HpyF3I CTNAG 5 cut(s) 270, 1883, 1917, 1935, 2165
HpySE526I ACGT 4 cut(s) 104, 218, 612, 1650
Hsp92I GRCGYC 2 cut(s) 737, 1679
Hsp92II CATG 9 cut(s) 95, 121, 161, 211, 239, 650, 1639, 1726, 1823
HspAI GCGC 5 cut(s) 737, 920, 989, 1011, 1566
KasI GGCGCC 1 cut(s) 736
Kpn2I TCCGGA 2 cut(s) 346, 1504
Ksp22I TGATCA 1 cut(s) 411
KspI CCGCGG 2 cut(s) 918, 1704
Kzo9I GATC 7 cut(s) 411, 863, 1159, 1825, 1861, 1920, 2132
LguI GCTCTTC 1 cut(s) 1308
LmnI GCTCC 4 cut(s) 445, 692, 902, 1695
LweI GCATC 6 cut(s) 20, 1120, 1318, 1403, 2057, 2167
MaeII ACGT 4 cut(s) 104, 218, 612, 1650
MaeIII GTNAC 9 cut(s) 31, 381, 624, 685, 1217, 1370, 1498, 1952, 2203
MalI GATC 7 cut(s) 413, 865, 1161, 1827, 1863, 1922, 2134
MboI GATC 7 cut(s) 411, 863, 1159, 1825, 1861, 1920, 2132
MflI RGATCY 1 cut(s) 1825
MhlI GDGCHC 4 cut(s) 450, 1662, 1700, 2095
MlsI TGGCCA 3 cut(s) 645, 755, 1359
MluCI AATT 6 cut(s) 294, 1067, 1419, 1492, 1903, 1987
MluNI TGGCCA 3 cut(s) 645, 755, 1359
Mly113I GGCGCC 1 cut(s) 737
MlyI GAGTC 4 cut(s) 337, 1258, 1949, 2068
MmeI TCCRAC 2 cut(s) 1433, 1552
Mox20I TGGCCA 3 cut(s) 645, 755, 1359
Mph1103I ATGCAT 1 cut(s) 1333
MroI TCCGGA 2 cut(s) 346, 1504
MroXI GAANNNNTTC 1 cut(s) 1943
MscI TGGCCA 3 cut(s) 645, 755, 1359
MseI TTAA 2 cut(s) 1710, 2060
MslI CAYNNNNRTG 4 cut(s) 63, 116, 434, 1653
Msp20I TGGCCA 3 cut(s) 645, 755, 1359
MspA1I CMGCKG 3 cut(s) 203, 917, 1703
MspR9I CCNGG 8 cut(s) 291, 1037, 1173, 1201, 1536, 1848, 2096, 2305
MvaI CCWGG 4 cut(s) 291, 1536, 1848, 2305
MvnI CGCG 4 cut(s) 140, 917, 1664, 1703
NarI GGCGCC 1 cut(s) 737
NciI CCSGG 4 cut(s) 1037, 1173, 1201, 2096
NcoI CCATGG 2 cut(s) 235, 646
NdeI CATATG 1 cut(s) 1306
NdeII GATC 7 cut(s) 411, 863, 1159, 1825, 1861, 1920, 2132
NheI GCTAGC 2 cut(s) 695, 2216
NlaIII CATG 9 cut(s) 95, 121, 161, 211, 239, 650, 1639, 1726, 1823
NlaIV GGNNCC 4 cut(s) 738, 1697, 1827, 2092
NmeAIII GCCGAG 2 cut(s) 286, 510
NmuCI GTSAC 6 cut(s) 624, 1217, 1370, 1498, 1952, 2203
NsiI ATGCAT 1 cut(s) 1333
NspI RCATGY 3 cut(s) 95, 211, 1823
OliI CACNNNNGTG 3 cut(s) 63, 434, 1653
PaeR7I CTCGAG 1 cut(s) 747
PagI TCATGA 1 cut(s) 1722
PciSI GCTCTTC 1 cut(s) 1308
PdmI GAANNNNTTC 1 cut(s) 1943
PfeI GAWTC 6 cut(s) 197, 617, 1117, 1183, 1400, 2159
PflFI GACNNNGTC 1 cut(s) 158
PinAI ACCGGT 1 cut(s) 1459
PleI GAGTC 4 cut(s) 337, 1257, 1949, 2068
PluTI GGCGCC 1 cut(s) 740
PmaCI CACGTG 1 cut(s) 1651
PmlI CACGTG 1 cut(s) 1651
PpsI GAGTC 4 cut(s) 337, 1257, 1949, 2068
Ppu21I YACGTR 2 cut(s) 219, 1651
PshAI GACNNNNGTC 1 cut(s) 468
Psp124BI GAGCTC 1 cut(s) 450
Psp6I CCWGG 4 cut(s) 289, 1534, 1846, 2303
PspCI CACGTG 1 cut(s) 1651
PspFI CCCAGC 2 cut(s) 1553, 1814
PspGI CCWGG 4 cut(s) 289, 1534, 1846, 2303
PspN4I GGNNCC 4 cut(s) 738, 1697, 1827, 2092
PspPI GGNCC 6 cut(s) 476, 596, 959, 1487, 1574, 1666
PspXI VCTCGAGB 1 cut(s) 747
PsuI RGATCY 1 cut(s) 1825
PsyI GACNNNGTC 1 cut(s) 158
PvuII CAGCTG 1 cut(s) 203
RseI CAYNNNNRTG 4 cut(s) 63, 116, 434, 1653
SacI GAGCTC 1 cut(s) 450
SacII CCGCGG 2 cut(s) 918, 1704
SapI GCTCTTC 1 cut(s) 1308
SaqAI TTAA 2 cut(s) 1710, 2060
Sau3AI GATC 7 cut(s) 411, 863, 1159, 1825, 1861, 1920, 2132
Sau96I GGNCC 6 cut(s) 476, 596, 959, 1487, 1574, 1666
ScaI AGTACT 2 cut(s) 1512, 1753
SchI GAGTC 4 cut(s) 337, 1258, 1949, 2068
ScrFI CCNGG 8 cut(s) 291, 1037, 1173, 1201, 1536, 1848, 2096, 2305
SduI GDGCHC 4 cut(s) 450, 1662, 1700, 2095
SfaNI GCATC 6 cut(s) 20, 1120, 1318, 1403, 2057, 2167
SfcI CTRYAG 1 cut(s) 1344
SfoI GGCGCC 1 cut(s) 738
Sfr274I CTCGAG 1 cut(s) 747
Sfr303I CCGCGG 2 cut(s) 918, 1704
SgrAI CRCCGGYG 1 cut(s) 431
SgrBI CCGCGG 2 cut(s) 918, 1704
SinI GGWCC 5 cut(s) 476, 596, 959, 1487, 1574
SlaI CTCGAG 1 cut(s) 747
SmiMI CAYNNNNRTG 4 cut(s) 63, 116, 434, 1653
SmlI CTYRAG 2 cut(s) 747, 1957
SmoI CTYRAG 2 cut(s) 747, 1957
Sse9I AATT 6 cut(s) 294, 1067, 1419, 1492, 1903, 1987
SspDI GGCGCC 1 cut(s) 736
SstI GAGCTC 1 cut(s) 450
StyD4I CCNGG 8 cut(s) 289, 1035, 1171, 1199, 1534, 1846, 2094, 2303
StyI CCWWGG 6 cut(s) 235, 646, 703, 992, 1051, 2029
TaaI ACNGT 5 cut(s) 65, 229, 892, 1006, 2203
TaiI ACGT 4 cut(s) 107, 221, 615, 1653
TaqI TCGA 9 cut(s) 14, 147, 279, 533, 748, 1151, 1767, 1803, 2114
TaqII GACCGA 2 cut(s) 1236, 1504
TasI AATT 6 cut(s) 294, 1067, 1419, 1492, 1903, 1987
TatI WGTACW 3 cut(s) 1510, 1751, 2064
TauI GCSGC 6 cut(s) 674, 742, 917, 920, 1667, 1706
TfiI GAWTC 6 cut(s) 197, 617, 1117, 1183, 1400, 2159
Tru1I TTAA 2 cut(s) 1710, 2060
Tru9I TTAA 2 cut(s) 1710, 2060
TscAI CASTG 8 cut(s) 472, 486, 1836, 2130, 2142, 2169, 2179, 2206
TseFI GTSAC 6 cut(s) 624, 1217, 1370, 1498, 1952, 2203
Tsp45I GTSAC 6 cut(s) 624, 1217, 1370, 1498, 1952, 2203
TspDTI ATGAA 3 cut(s) 17, 621, 1556
TspGWI ACGGA 3 cut(s) 10, 1451, 2137
TspRI CASTG 8 cut(s) 472, 486, 1836, 2130, 2142, 2169, 2179, 2206
Tth111I GACNNNGTC 1 cut(s) 158
VpaK11BI GGWCC 5 cut(s) 476, 596, 959, 1487, 1574
XagI CCTNNNNNAGG 1 cut(s) 1673
XapI RAATTY 3 cut(s) 294, 1067, 1492
XbaI TCTAGA 2 cut(s) 1481, 2011
XceI RCATGY 3 cut(s) 95, 211, 1823
XhoI CTCGAG 1 cut(s) 747
XmiI GTMKAC 1 cut(s) 1857
XmnI GAANNNNTTC 1 cut(s) 1943
ZrmI AGTACT 2 cut(s) 1512, 1753
Zsp2I ATGCAT 1 cut(s) 1333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.