pycom08g07160

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0001634
Physical Location & Seq
Forward (+)
5765397 .. 5767651
2255 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g07160.1

Sequence Viewer

Length: 2103 bp
ATGCTTTACACTTACAAGACCATAATCCACGGCTTCGCCACTAAGCTAACCGCCGAGGAAGCTGAGCTGCTTGAGAAGCAGTCGGGAATTCTGTCCGTTCTGCCCGAAAGGAGATACGAGCTGCATACAACTCGGTCGCCCGAGTTTCTTGGATTGGGAAAGAGTGAAGCCCTCTTGCCGGCGTCGGGTACAGTGAGTGACGTGATTGTCGGAGTGGTGGACACTGGCGTGTGGCCTGAGCTCAAAAGCTACGATGACACAGGTCTCGCGGCAGTACCAAGTGGCTGGAAAGGGGTGTGTGAGGCGGGAACAAACTTCAGCTCCTCAAACTGCAACCGGAAACTCATAGGCGCGAGGTTTTTCTCGAGAGGGTATGAAGCTACAGTTGGACCGATCGATGTGAAAGCAGAATCGAAATCACCACGAGATGATGATGGTCACGGAACTCACACCTCATCCACTGCAACCGGTTCAGCAGTTTCAGGAGCAAGCCTCTTTGGTTATGCTTCGGGGACAGCGCGAGGGATGGCGCCACAAGCTAGATTGGCCACGTACAAGGCGTGCTGGCTTGGTGGGTGTTTCGGCTCTGATATCACAGCCGCAATGGAGAAGGCCGTGGAAGACGGTGTCGATGTTTTATCTTTGTCTATTGGGGGATCACAGTCTGAATATTACAGAGACACTGTTGCTATTGGAGCTTTCTCTGCCGCGGCGCAGGGATCGATCCTTGTGTCTTGTTCAGCTGGTAATGGAGGACCGGAAGCAGGGAGTCTGTCCAACGTTGCGCCTTGGATAACTACAGTGGGTGCTGGAACATTAGACAGGGATTTCCCGGCTTTTGTTAGCCTCGGAAGTGCAAAGAAATACAGAGGTATATCGCTCTATAGGGGAACATCCTTATCTAGTGGATTGCTTCCGCTTGTATATGCTGGCAACGCAAGTGAGTCCTCCATGGGCGGGCTATGCGCTCCAGAAAGTCTGATTCCCGGAAAGGTTGCAGGGAAAATTGTGGTATGTGATCGAGGGGGAACCCCTAGGGTCCAAAAGAGTTTGGTGGTGAAAAAGGCCGGCGGTTTAGGGATGATTTTGACGAACACGGATGCTTACGGAGAAGAACTTGTTGCGGATGCATATCTACTGCCTACAGCAGCAGTTGGTGCGAAAGCTGGGGCTGCAATAAAAAGCTATATTGCCTCGCAATCTAATCCTACCGCCACAATTGCGCTTGGAGACACGGAGTTAGATGTGCAGCCCTCGCCCGTGGTGGCATCATTCAGCTCAAGAGGACCGAACCTAGTCACCCCGGAATTACTCAAACCAGACCTAATAGCACCGGGAGTAAATATCCTTGCTGGGTGGACTGGTGCGATTGGACCAACCGGACTAGCCGAAGACAAGAGGCATGTCACCTTCAACATCATTTCAGGTACTTCCATGTCATGCCCGCATGTGAGTGGTTTGGCCGCCCTTGTCAAGGCGGCTCATCCAGGATGGAGCCCTGCAGCCATTAAGTCTGCACTCATGACTACATCCTACACAGCATACAAAACTGGAGAAACCATCATAGATGTAGCAACTGGAAATCCTGCAACGCCGTTTGATTATGGTGCTGGGCATGTGGATCCGGTGGCAGCCCTCGACCCTGGCCTTGTTTATGATAATGCGGTCGAGGACTACTTCAGCTTCCTCTACTTCACTTGCGATACAAGAAAGAAATACAGCGTCGGAGATTTCAACTACCCGTCTTTTGCTGTTCCTCTAGAAACATCTTCTGGCAGAGGGGGTGGAACAGGTGCTTCAACAACTGTAAAACACACAAGGACACTGACCAATGTGGGTACCCCAGGAACATACAAGGTCACGATATCTTCACAGGCTCAAGCATACGAGAAGAAAACATATACCGTGACTTTTGTTGCTGGTTCTTCGCCATCCGGTACAAGCAGCTTTGCTCGGTTGGTATGGTCCGATGGGAAACGCACAGTAGGTAGTCCAATTATCAGGGGTTTTAATGTTCCTGCAAAAAACCCAATTGTATATTTCTTAGTATGTTGTGAAATTATCATGTACCTCTCTTGTATCGTAACAACTGCGCGAACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

701

Amino Acids

72.54

Weight (kDa)

6.76

Isoelectric Point (pI)

29.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 1 - 42 3.4e-09 Peptidase inhibitor I9
Peptidase_S8 PF00082 67 - 538 1.1e-45 Subtilase family
PA PF02225 306 - 393 4.8e-12 PA domain
fn3_6 PF17766 577 - 667 3.9e-11 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 206
Acc65I GGTACC 1 cut(s) 1838
AccB1I GGYRCC 2 cut(s) 529, 1838
AccII CGCG 5 cut(s) 269, 353, 520, 710, 2095
AclI AACGTT 1 cut(s) 780
AclWI GGATC 5 cut(s) 664, 718, 727, 1616, 1629
AcoI YGGCCR 2 cut(s) 546, 1461
AcsI RAATTY 1 cut(s) 87
AcuI CTGAAG 2 cut(s) 301, 1663
AcyI GRCGYC 2 cut(s) 182, 530
AfaI GTAC 7 cut(s) 190, 276, 554, 1429, 1840, 1939, 2069
AfiI CCNNNNNNNGG 5 cut(s) 178, 185, 764, 957, 1474
AgeI ACCGGT 1 cut(s) 467
AgsI TTSAA 3 cut(s) 1414, 1735, 1800
AjiI CACGTC 1 cut(s) 202
AjnI CCWGG 3 cut(s) 1486, 1642, 1843
AjuI GAANNNNNNNTTGG 2 cut(s) 369, 401
AleI CACNNNNGTG 1 cut(s) 227
Alw21I GWGCWC 1 cut(s) 243
Alw26I GTCTC 3 cut(s) 269, 672, 1224
AlwI GGATC 5 cut(s) 664, 718, 727, 1616, 1629
Ama87I CYCGRG 2 cut(s) 140, 364
AoxI GGCC 6 cut(s) 233, 546, 612, 1065, 1461, 1645
ApeKI GCWGC 8 cut(s) 67, 121, 1148, 1172, 1249, 1502, 1631, 1944
ApoI RAATTY 1 cut(s) 87
AsiGI ACCGGT 1 cut(s) 467
Asp718I GGTACC 1 cut(s) 1838
AspA2I CCTAGG 1 cut(s) 1034
AspLEI GCGC 8 cut(s) 353, 520, 532, 715, 787, 968, 1225, 2095
AspS9I GGNCC 6 cut(s) 389, 755, 1039, 1286, 1373, 1965
AsuC2I CCSGG 4 cut(s) 833, 987, 1304, 1335
AsuHPI GGTGA 4 cut(s) 411, 1069, 1291, 1399
AvaI CYCGRG 2 cut(s) 140, 364
AvaII GGWCC 6 cut(s) 389, 755, 1039, 1286, 1373, 1965
AvrII CCTAGG 1 cut(s) 1034
BalI TGGCCA 1 cut(s) 548
BamHI GGATCC 1 cut(s) 1621
BanI GGYRCC 2 cut(s) 529, 1838
BanII GRGCYC 2 cut(s) 243, 1499
BauI CACGAG 1 cut(s) 423
BbsI GAAGAC 2 cut(s) 627, 1398
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 8 cut(s) 54, 108, 1159, 1160, 1261, 1514, 1643, 1956
BccI CCATC 6 cut(s) 428, 520, 1485, 1568, 1939, 1964
BceAI ACGGC 3 cut(s) 46, 599, 1579
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciT130I CCWGG 3 cut(s) 1488, 1644, 1845
BcnI CCSGG 4 cut(s) 833, 987, 1304, 1335
BcoDI GTCTC 3 cut(s) 269, 672, 1224
BfaI CTAG 6 cut(s) 540, 903, 1035, 1295, 1385, 1760
BfmI CTRYAG 5 cut(s) 381, 798, 883, 1143, 1500
BfoI RGCGCY 1 cut(s) 533
BlnI CCTAGG 1 cut(s) 1034
BlpI GCTNAGC 1 cut(s) 63
Bme1390I CCNGG 7 cut(s) 833, 987, 1304, 1335, 1488, 1644, 1845
Bme18I GGWCC 6 cut(s) 389, 755, 1039, 1286, 1373, 1965
BmeT110I CYCGRG 2 cut(s) 140, 364
BmgBI CACGTC 1 cut(s) 202
BmgT120I GGNCC 6 cut(s) 389, 755, 1039, 1286, 1373, 1965
BmiI GGNNCC 6 cut(s) 531, 1030, 1040, 1496, 1623, 1840
BmrFI CCNGG 7 cut(s) 833, 987, 1304, 1335, 1488, 1644, 1845
BmsI GCATC 3 cut(s) 1090, 1117, 1277
BoxI GACNNNNGTC 1 cut(s) 261
BpiI GAAGAC 2 cut(s) 627, 1398
BplI GAGNNNNNCTC 2 cut(s) 477, 509
BpmI CTGGAG 2 cut(s) 954, 1572
Bpu10I CCTNAGC 1 cut(s) 237
Bpu1102I GCTNAGC 1 cut(s) 63
BpuEI CTTGAG 3 cut(s) 92, 1264, 1863
BpuMI CCSGG 4 cut(s) 833, 987, 1304, 1335
Bsa29I ATCGAT 2 cut(s) 396, 722
BsaAI YACGTR 1 cut(s) 552
BsaBI GATNNNNATC 1 cut(s) 1131
BsaHI GRCGYC 2 cut(s) 182, 530
BsaI GGTCTC 1 cut(s) 269
BsaWI WCCGGW 6 cut(s) 336, 467, 757, 1379, 1624, 1934
BsaXI ACNNNNNCTCC 2 cut(s) 305, 335
Bsc4I CCNNNNNNNGG 5 cut(s) 178, 185, 764, 957, 1474
Bse118I RCCGGY 3 cut(s) 178, 467, 1067
Bse1I ACTGG 4 cut(s) 229, 1366, 1555, 1582
Bse3DI GCAATG 1 cut(s) 609
Bse8I GATNNNNATC 1 cut(s) 1131
BseBI CCWGG 3 cut(s) 1488, 1644, 1845
BseCI ATCGAT 2 cut(s) 396, 722
BseJI GATNNNNATC 1 cut(s) 1131
BseLI CCNNNNNNNGG 5 cut(s) 178, 185, 764, 957, 1474
BseMI GCAATG 1 cut(s) 609
BseMII CTCAG 2 cut(s) 54, 228
BseNI ACTGG 4 cut(s) 229, 1366, 1555, 1582
BseRI GAGGAG 1 cut(s) 313
BseXI GCAGC 8 cut(s) 54, 108, 1159, 1160, 1261, 1514, 1643, 1956
BseYI CCCAGC 3 cut(s) 1166, 1352, 1610
BsgI GTGCAG 2 cut(s) 1268, 1500
Bsh1236I CGCG 5 cut(s) 269, 353, 520, 710, 2095
Bsh1285I CGRYCG 3 cut(s) 137, 396, 1668
BshFI GGCC 6 cut(s) 235, 548, 614, 1067, 1463, 1647
BshNI GGYRCC 2 cut(s) 529, 1838
BshTI ACCGGT 1 cut(s) 467
BshVI ATCGAT 2 cut(s) 396, 722
BsiEI CGRYCG 3 cut(s) 137, 396, 1668
BsiHKAI GWGCWC 1 cut(s) 243
BsiHKCI CYCGRG 2 cut(s) 140, 364
BslFI GGGAC 1 cut(s) 526
BslI CCNNNNNNNGG 5 cut(s) 178, 185, 764, 957, 1474
BsmAI GTCTC 3 cut(s) 269, 672, 1224
BsmFI GGGAC 1 cut(s) 526
BsnI GGCC 6 cut(s) 235, 548, 614, 1067, 1463, 1647
Bso31I GGTCTC 1 cut(s) 269
BsoBI CYCGRG 2 cut(s) 140, 364
Bsp1286I GDGCHC 2 cut(s) 243, 1499
Bsp143I GATC 6 cut(s) 393, 656, 719, 723, 1018, 1621
Bsp1720I GCTNAGC 1 cut(s) 63
Bsp19I CCATGG 1 cut(s) 951
BspANI GGCC 6 cut(s) 235, 548, 614, 1067, 1463, 1647
BspCNI CTCAG 2 cut(s) 55, 229
BspDI ATCGAT 2 cut(s) 396, 722
BspFNI CGCG 5 cut(s) 269, 353, 520, 710, 2095
BspHI TCATGA 1 cut(s) 1521
BspLI GGNNCC 6 cut(s) 531, 1030, 1040, 1496, 1623, 1840
BspMAI CTGCAG 1 cut(s) 1504
BspPI GGATC 5 cut(s) 664, 718, 727, 1616, 1629
BspT107I GGYRCC 2 cut(s) 529, 1838
BspTNI GGTCTC 1 cut(s) 269
BsrDI GCAATG 1 cut(s) 609
BsrFI RCCGGY 3 cut(s) 178, 467, 1067
BsrI ACTGG 4 cut(s) 229, 1366, 1555, 1582
BssAI RCCGGY 3 cut(s) 178, 467, 1067
BssMI GATC 6 cut(s) 393, 656, 719, 723, 1018, 1621
BssNI GRCGYC 2 cut(s) 182, 530
BssSI CACGAG 1 cut(s) 423
BssT1I CCWWGG 3 cut(s) 788, 951, 1034
Bst2BI CACGAG 1 cut(s) 423
Bst2UI CCWGG 3 cut(s) 1488, 1644, 1845
Bst4CI ACNGT 9 cut(s) 193, 385, 626, 663, 685, 802, 1807, 1906, 1984
BstACI GRCGYC 2 cut(s) 182, 530
BstAPI GCANNNNNTGC 1 cut(s) 1157
BstBAI YACGTR 1 cut(s) 552
BstC8I GCNNGC 8 cut(s) 180, 490, 562, 566, 931, 959, 1069, 1445
BstDEI CTNAG 4 cut(s) 42, 63, 237, 2044
BstDSI CCRYGG 5 cut(s) 28, 615, 708, 951, 1260
BstENI CCTNNNNNAGG 1 cut(s) 1472
BstFNI CGCG 5 cut(s) 269, 353, 520, 710, 2095
BstH2I RGCGCY 1 cut(s) 533
BstHHI GCGC 8 cut(s) 353, 520, 532, 715, 787, 968, 1225, 2095
BstKTI GATC 6 cut(s) 396, 659, 722, 726, 1021, 1624
BstMAI GTCTC 3 cut(s) 269, 672, 1224
BstMBI GATC 6 cut(s) 393, 656, 719, 723, 1018, 1621
BstMCI CGRYCG 3 cut(s) 137, 396, 1668
BstNI CCWGG 3 cut(s) 1488, 1644, 1845
BstNSI RCATGY 3 cut(s) 1406, 1451, 1619
BstPAI GACNNNNGTC 1 cut(s) 261
BstSCI CCNGG 7 cut(s) 831, 985, 1302, 1333, 1486, 1642, 1843
BstSFI CTRYAG 5 cut(s) 381, 798, 883, 1143, 1500
BstUI CGCG 5 cut(s) 269, 353, 520, 710, 2095
BstV1I GCAGC 8 cut(s) 54, 108, 1159, 1160, 1261, 1514, 1643, 1956
BstV2I GAAGAC 2 cut(s) 627, 1398
BstX2I RGATCY 1 cut(s) 1621
BstXI CCANNNNNNTGG 1 cut(s) 285
BstYI RGATCY 1 cut(s) 1621
Bsu15I ATCGAT 2 cut(s) 396, 722
BsuRI GGCC 6 cut(s) 235, 548, 614, 1067, 1463, 1647
BsuTUI ATCGAT 2 cut(s) 396, 722
BtgI CCRYGG 5 cut(s) 28, 615, 708, 951, 1260
BtrI CACGTC 1 cut(s) 202
BtsI GCAGTG 1 cut(s) 459
BtsIMutI CAGTG 6 cut(s) 198, 222, 459, 681, 807, 1823
Cac8I GCNNGC 8 cut(s) 180, 490, 562, 566, 931, 959, 1069, 1445
CciI TCATGA 1 cut(s) 1521
CfoI GCGC 8 cut(s) 353, 520, 532, 715, 787, 968, 1225, 2095
Cfr10I RCCGGY 3 cut(s) 178, 467, 1067
Cfr13I GGNCC 6 cut(s) 389, 755, 1039, 1286, 1373, 1965
Cfr42I CCGCGG 1 cut(s) 711
ClaI ATCGAT 2 cut(s) 396, 722
CseI GACGC 2 cut(s) 171, 1711
Csp6I GTAC 7 cut(s) 189, 275, 553, 1428, 1839, 1938, 2068
CspAI ACCGGT 1 cut(s) 467
CviAII CATG 8 cut(s) 952, 1403, 1435, 1440, 1448, 1522, 1616, 2065
CviQI GTAC 7 cut(s) 189, 275, 553, 1428, 1839, 1938, 2068
DdeI CTNAG 4 cut(s) 42, 63, 237, 2044
DinI GGCGCC 1 cut(s) 531
DpnI GATC 6 cut(s) 395, 658, 721, 725, 1020, 1623
DpnII GATC 6 cut(s) 393, 656, 719, 723, 1018, 1621
DrdI GACNNNNNNGTC 1 cut(s) 206
DseDI GACNNNNNNGTC 1 cut(s) 206
EaeI YGGCCR 2 cut(s) 546, 1461
Ecl136II GAGCTC 1 cut(s) 241
Eco130I CCWWGG 3 cut(s) 788, 951, 1034
Eco24I GRGCYC 2 cut(s) 243, 1499
Eco31I GGTCTC 1 cut(s) 269
Eco32I GATATC 2 cut(s) 592, 1866
Eco47I GGWCC 6 cut(s) 389, 755, 1039, 1286, 1373, 1965
Eco53kI GAGCTC 1 cut(s) 241
Eco57I CTGAAG 2 cut(s) 301, 1663
Eco88I CYCGRG 2 cut(s) 140, 364
EcoICRI GAGCTC 1 cut(s) 241
EcoNI CCTNNNNNAGG 1 cut(s) 1472
EcoRI GAATTC 1 cut(s) 87
EcoRII CCWGG 3 cut(s) 1486, 1642, 1843
EcoRV GATATC 2 cut(s) 592, 1866
EcoT14I CCWWGG 3 cut(s) 788, 951, 1034
EcoT22I ATGCAT 1 cut(s) 1132
EcoT38I GRGCYC 2 cut(s) 243, 1499
EgeI GGCGCC 1 cut(s) 531
EheI GGCGCC 1 cut(s) 531
ErhI CCWWGG 3 cut(s) 788, 951, 1034
FaeI CATG 8 cut(s) 955, 1406, 1438, 1443, 1451, 1525, 1619, 2068
FaqI GGGAC 1 cut(s) 526
FatI CATG 8 cut(s) 951, 1402, 1434, 1439, 1447, 1521, 1615, 2064
FauI CCCGC 3 cut(s) 298, 950, 1452
FriOI GRGCYC 2 cut(s) 243, 1499
FspBI CTAG 6 cut(s) 540, 903, 1035, 1295, 1385, 1760
GlaI GCGC 8 cut(s) 352, 519, 531, 714, 786, 967, 1224, 2094
GsaI CCCAGC 3 cut(s) 1170, 1356, 1614
GsuI CTGGAG 2 cut(s) 954, 1572
HaeII RGCGCY 1 cut(s) 533
HaeIII GGCC 6 cut(s) 235, 548, 614, 1067, 1463, 1647
HgaI GACGC 2 cut(s) 171, 1711
HhaI GCGC 8 cut(s) 353, 520, 532, 715, 787, 968, 1225, 2095
Hin1I GRCGYC 2 cut(s) 182, 530
Hin1II CATG 8 cut(s) 955, 1406, 1438, 1443, 1451, 1525, 1619, 2068
Hin6I GCGC 8 cut(s) 351, 518, 530, 713, 785, 966, 1223, 2093
HinP1I GCGC 8 cut(s) 351, 518, 530, 713, 785, 966, 1223, 2093
HinfI GANTC 4 cut(s) 410, 769, 944, 982
HphI GGTGA 4 cut(s) 411, 1069, 1291, 1399
Hpy166II GTNNAC 2 cut(s) 220, 1359
Hpy188I TCNGA 7 cut(s) 212, 589, 667, 851, 981, 1727, 1969
Hpy188III TCNNGA 9 cut(s) 84, 364, 366, 483, 971, 1281, 1522, 1760, 1861
Hpy8I GTNNAC 2 cut(s) 220, 1359
Hpy99I CGWCG 2 cut(s) 187, 1727
HpyAV CCTTC 2 cut(s) 604, 1420
HpyCH4III ACNGT 9 cut(s) 193, 385, 626, 663, 685, 802, 1807, 1906, 1984
HpyCH4IV ACGT 4 cut(s) 201, 551, 780, 2099
HpyF3I CTNAG 4 cut(s) 42, 63, 237, 2044
HpySE526I ACGT 4 cut(s) 201, 551, 780, 2099
Hsp92I GRCGYC 2 cut(s) 182, 530
Hsp92II CATG 8 cut(s) 955, 1406, 1438, 1443, 1451, 1525, 1619, 2068
HspAI GCGC 8 cut(s) 351, 518, 530, 713, 785, 966, 1223, 2093
KasI GGCGCC 1 cut(s) 529
KpnI GGTACC 1 cut(s) 1842
KroI GCCGGC 2 cut(s) 178, 1067
KroNI GCCGGC 2 cut(s) 180, 1069
KspI CCGCGG 1 cut(s) 711
Kzo9I GATC 6 cut(s) 393, 656, 719, 723, 1018, 1621
LmnI GCTCC 5 cut(s) 326, 485, 695, 973, 1494
Lsp1109I GCAGC 8 cut(s) 54, 108, 1159, 1160, 1261, 1514, 1643, 1956
LweI GCATC 3 cut(s) 1090, 1117, 1277
MaeI CTAG 6 cut(s) 540, 903, 1035, 1295, 1385, 1760
MaeII ACGT 4 cut(s) 201, 551, 780, 2099
MaeIII GTNAC 7 cut(s) 197, 437, 1297, 1405, 1858, 1906, 2083
MalI GATC 6 cut(s) 395, 658, 721, 725, 1020, 1623
MboI GATC 6 cut(s) 393, 656, 719, 723, 1018, 1621
MboII GAAGA 7 cut(s) 632, 1124, 1403, 1761, 1860, 1903, 1917
MfeI CAATTG 2 cut(s) 1218, 2031
MflI RGATCY 1 cut(s) 1621
MhlI GDGCHC 2 cut(s) 243, 1499
MlsI TGGCCA 1 cut(s) 548
MluCI AATT 7 cut(s) 87, 1005, 1218, 1307, 1995, 2031, 2058
MluNI TGGCCA 1 cut(s) 548
Mly113I GGCGCC 1 cut(s) 530
MlyI GAGTC 2 cut(s) 778, 953
MmeI TCCRAC 4 cut(s) 190, 367, 801, 1705
Mox20I TGGCCA 1 cut(s) 548
Mph1103I ATGCAT 1 cut(s) 1132
MroNI GCCGGC 2 cut(s) 178, 1067
MscI TGGCCA 1 cut(s) 548
MseI TTAA 2 cut(s) 1509, 2010
MslI CAYNNNNRTG 2 cut(s) 227, 1452
Msp20I TGGCCA 1 cut(s) 548
MspA1I CMGCKG 2 cut(s) 710, 743
MspR9I CCNGG 7 cut(s) 833, 987, 1304, 1335, 1488, 1644, 1845
MunI CAATTG 2 cut(s) 1218, 2031
MvaI CCWGG 3 cut(s) 1488, 1644, 1845
MvnI CGCG 5 cut(s) 269, 353, 520, 710, 2095
NaeI GCCGGC 2 cut(s) 180, 1069
NarI GGCGCC 1 cut(s) 530
NciI CCSGG 4 cut(s) 833, 987, 1304, 1335
NcoI CCATGG 1 cut(s) 951
NdeII GATC 6 cut(s) 393, 656, 719, 723, 1018, 1621
NgoMIV GCCGGC 2 cut(s) 178, 1067
NlaIII CATG 8 cut(s) 955, 1406, 1438, 1443, 1451, 1525, 1619, 2068
NlaIV GGNNCC 6 cut(s) 531, 1030, 1040, 1496, 1623, 1840
NmeAIII GCCGAG 1 cut(s) 79
NmuCI GTSAC 6 cut(s) 197, 437, 1297, 1405, 1858, 1906
NsiI ATGCAT 1 cut(s) 1132
NspI RCATGY 3 cut(s) 1406, 1451, 1619
OliI CACNNNNGTG 1 cut(s) 227
PaeR7I CTCGAG 1 cut(s) 364
PagI TCATGA 1 cut(s) 1521
PcsI WCGNNNNNNNCGW 1 cut(s) 557
PdiI GCCGGC 2 cut(s) 180, 1069
PfeI GAWTC 2 cut(s) 410, 982
PflFI GACNNNGTC 1 cut(s) 626
PfoI TCCNGGA 2 cut(s) 985, 1486
PinAI ACCGGT 1 cut(s) 467
Ple19I CGATCG 1 cut(s) 396
PleI GAGTC 2 cut(s) 777, 952
PluTI GGCGCC 1 cut(s) 533
PpsI GAGTC 2 cut(s) 777, 952
Ppu21I YACGTR 1 cut(s) 552
PshAI GACNNNNGTC 1 cut(s) 261
Psp124BI GAGCTC 1 cut(s) 243
Psp1406I AACGTT 1 cut(s) 780
Psp6I CCWGG 3 cut(s) 1486, 1642, 1843
PspFI CCCAGC 3 cut(s) 1166, 1352, 1610
PspGI CCWGG 3 cut(s) 1486, 1642, 1843
PspN4I GGNNCC 6 cut(s) 531, 1030, 1040, 1496, 1623, 1840
PspPI GGNCC 6 cut(s) 389, 755, 1039, 1286, 1373, 1965
PstI CTGCAG 1 cut(s) 1504
PsuI RGATCY 1 cut(s) 1621
PsyI GACNNNGTC 1 cut(s) 626
PvuI CGATCG 1 cut(s) 396
PvuII CAGCTG 1 cut(s) 743
RsaI GTAC 7 cut(s) 190, 276, 554, 1429, 1840, 1939, 2069
RsaNI GTAC 7 cut(s) 189, 275, 553, 1428, 1839, 1938, 2068
RseI CAYNNNNRTG 2 cut(s) 227, 1452
SacI GAGCTC 1 cut(s) 243
SacII CCGCGG 1 cut(s) 711
SaqAI TTAA 2 cut(s) 1509, 2010
Sau3AI GATC 6 cut(s) 393, 656, 719, 723, 1018, 1621
Sau96I GGNCC 6 cut(s) 389, 755, 1039, 1286, 1373, 1965
SchI GAGTC 2 cut(s) 778, 953
ScrFI CCNGG 7 cut(s) 833, 987, 1304, 1335, 1488, 1644, 1845
SduI GDGCHC 2 cut(s) 243, 1499
SfaNI GCATC 3 cut(s) 1090, 1117, 1277
SfcI CTRYAG 5 cut(s) 381, 798, 883, 1143, 1500
SfoI GGCGCC 1 cut(s) 531
Sfr274I CTCGAG 1 cut(s) 364
Sfr303I CCGCGG 1 cut(s) 711
SgrBI CCGCGG 1 cut(s) 711
SinI GGWCC 6 cut(s) 389, 755, 1039, 1286, 1373, 1965
SlaI CTCGAG 1 cut(s) 364
SmiMI CAYNNNNRTG 2 cut(s) 227, 1452
SmlI CTYRAG 4 cut(s) 71, 364, 1279, 1878
SmoI CTYRAG 4 cut(s) 71, 364, 1279, 1878
Sse9I AATT 7 cut(s) 87, 1005, 1218, 1307, 1995, 2031, 2058
SspDI GGCGCC 1 cut(s) 529
SspI AATATT 1 cut(s) 671
SspMI CTAG 6 cut(s) 540, 903, 1035, 1295, 1385, 1760
SstI GAGCTC 1 cut(s) 243
StyD4I CCNGG 7 cut(s) 831, 985, 1302, 1333, 1486, 1642, 1843
StyI CCWWGG 3 cut(s) 788, 951, 1034
TaaI ACNGT 9 cut(s) 193, 385, 626, 663, 685, 802, 1807, 1906, 1984
TaiI ACGT 4 cut(s) 204, 554, 783, 2102
TaqI TCGA 8 cut(s) 365, 396, 413, 630, 722, 1021, 1638, 1668
TaqII GACCGA 3 cut(s) 123, 406, 1303
TasI AATT 7 cut(s) 87, 1005, 1218, 1307, 1995, 2031, 2058
TauI GCSGC 6 cut(s) 272, 602, 710, 713, 1466, 1481
TfiI GAWTC 2 cut(s) 410, 982
Tru1I TTAA 2 cut(s) 1509, 2010
Tru9I TTAA 2 cut(s) 1509, 2010
TscAI CASTG 6 cut(s) 198, 229, 466, 688, 807, 1830
TseFI GTSAC 6 cut(s) 197, 437, 1297, 1405, 1858, 1906
TseI GCWGC 8 cut(s) 67, 121, 1148, 1172, 1249, 1502, 1631, 1944
Tsp45I GTSAC 6 cut(s) 197, 437, 1297, 1405, 1858, 1906
TspDTI ATGAA 1 cut(s) 390
TspGWI ACGGA 5 cut(s) 85, 456, 1112, 1122, 1250
TspRI CASTG 6 cut(s) 198, 229, 466, 688, 807, 1830
Tth111I GACNNNGTC 1 cut(s) 626
VpaK11BI GGWCC 6 cut(s) 389, 755, 1039, 1286, 1373, 1965
XagI CCTNNNNNAGG 1 cut(s) 1472
XapI RAATTY 1 cut(s) 87
XbaI TCTAGA 1 cut(s) 1759
XceI RCATGY 3 cut(s) 1406, 1451, 1619
XhoI CTCGAG 1 cut(s) 364
XmaJI CCTAGG 1 cut(s) 1034
XspI CTAG 6 cut(s) 540, 903, 1035, 1295, 1385, 1760
Zsp2I ATGCAT 1 cut(s) 1132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.