Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0001634

Physical Location & Seq

Genomic Coordinates Reverse (-)

18394573 .. 18396870

Gene Structure 2298 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000017570

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2298 bp

ATGGCCATGTCTAGTTTCTTGATGGTTGTTTTGTTTCTGGGTCTGGGTCTGGGCCATTTCGCCGTGGCGGAGGTGGAGGCGGAAGCTAGAAGCAGTCGGAGCACCTACATTATTCACATGGCGAAATCCGAAATGCCTGCGACTTTCGAGCACCACACTCACTGGTACGACGCGTCGCTCAAATCGGCCTCCGACTCGGCCGAGATGCTCTACACCTACACCAACGCCATCCACGGCTTCTCCACGCAGCTGACGCCGGAGGAGGCGGAGACGCTTAAGTTCCAACCCGGGGTTCTGTTCGTTCTTCCCGAGTTGAAGTACGAGCTCCACACCACTCGCACCCCCGAGTTTCTCGGACTCGACCAGAACAACCAGCTCTTCCCCGACTCGGTGTCTGCCAGTGACGTCATCGTCGGAGTCTTGGACACTGGAGTCTGGCCAGAAAGCAAGAGCTTTGATGACGCCGGACTCGGACCTGTTCCGGCGAGCTGGAAGGGCACGTGTGAAGTTGGCACTAGCTTCAATTCCTCGGCGTGTAATCGGAAGCTGATCGGGGCCAGGTACTTCGCCAAAGGCTACGAGGCCACATTAGGCCCCATCGACACTTCCAGAGAGTCCAGGTCCCCCAGAGACGACGACGGCCACGGAACCCACACCGCCACCACCGCGGCGGGGTCCGTCGTCGCCGGAGCGAGCCTGTTCGGATACGCTCCGGGATCCGCTCGTGGGATGGCCACGCGCGCCAGGATCGCCGCCTACAAAGTGTGCTGGATCGGCGGGTGTTTCAGCTCCGACATCTTGATGGCCATTGACCAAGCCATCGACGACAACGTCAACGTTTTGTCCATGTCACTCGGCGGCGGAATGTCCGACTATTTCAGGGACAGCGTGGCAATCGGAGCTTTCTCGGCGATGGAGAAGGGGATCTTGATCTCGTGCTCGGCCGGAAACGCGGGTCCCAGCGCGTACAGTTTGTCCAACTCGGCTCCGTGGATCACAACCGTAGGCGCGGGCACACTGGATCGCGACTTCCCGGCTTACCTCAGCCTCGGTGACGGTAAAAACATCTCAGGCGTTTCGCTTTACCCCCGTAATTCCGAGGCCACCGCTTTGCAAATGACGCCCTTTATTTACGCCGGTAACGCGAGTAACTCGACGTCCGGGAATCTCTGCATGATGGGAAGTTTGATACCTGAAAAAGTGAAGGGCAAAATAGTAATGTGCGACCGTGGAGTCAACTCCCGGGTCCAGAAAGGAACCGTGGTCAAAGCCGCCGGAGGAATCGGCATGGTCCTCTCCAACACCGCCGCAAACGGCGAGGAGCTGGTGGCCGACGCTCATTTGCTGCCGGCGACGGCGGTGGGCAAGAAAAACGGCGATGTCGTAAGGAGCTATCTGTTTTCGGATCCGAATCCAACGGCCGCGATCCTGTTCGAGGGCACCAAGGTCGGAATCGAGCCTTCTCCGGTAGTCGCGGCGTTCAGTTCCCGAGGCCCGAACGCGATCACGCCGGACATTCTGAAACCCGACATCGTCGCGCCAGGTGTCAACATCTTAGCTGGGTGGTCAGGCGCCGTCGGCCCCACCGGTCTGGCGGTCGACTCGCGGCGCGTGGCGTTCAACATCATCTCCGGCACGTCGATGTCCTGCCCGCACGTGAGCGGCCTCGCCGCGCTGCTCAAAGGGGCCCACCCGGACTGGAGCCCTGCCGCGATCCGATCGGCGCTGATGACCACCGCCTACACGGCCTACAAGAATGGACAGAAGTTGCAGGACGTGGCCACCGGAAAGCCATCGACGCCCTTCGATCACGGTGCTGGACACGTGGACCCCGTATCGGCCCTCAATCCGGGGCTGGTGTACGATCTAACGGTGGAGGATTATCTAAACTTTCTCTGTGCGTTGAACTACACCGAGTCCGAAATCACCAGCTTGGCTAAAAGAAAGTTTACTTGCGACGAAAGCAAGAGTTACAGCGTTAGGGATCTGAACTACCCTTCTTTTGCTGTAAATTTGGTGAGCGGCTCGTCGTCTTCGTCCACCGTATTGAAGTACTCGAGGACTCTCACCAACGTGGGCCCGGCCGGAACGTACAAGGCGACTGCGACCATGGATGACCCGTCGGTGAAGATTTCGGTGGAGCCAGAGTCGTTGAGTTTCGCCGCGACGAATGAGAAGAAGAGTTACACGGTGACGTTCGCGGTAACGGGGTCGAGATCATTGAGCAGCTTTGGGAGGCTCGAGTGGTCTGATGGGAAGCACATTGTTGGAAGTCCGATAGCAATCAGCCGGAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0006508 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009505 GO:0009791 GO:0009845 GO:0009987 GO:0010154 GO:0010191 GO:0010214 GO:0016787 GO:0017171 GO:0019538 GO:0022414 GO:0030312 GO:0032501 GO:0032502 GO:0032504 GO:0043170 GO:0044237 GO:0044238 GO:0044464 GO:0048046 GO:0048316 GO:0048359 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0061458 GO:0070011 GO:0071704 GO:0071944 GO:0080001 GO:0090351 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

766

Amino Acids

80.45

Weight (kDa)

5.88

Isoelectric Point (pI)

36.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	35 - 110	4e-19	Peptidase inhibitor I9
Peptidase_S8	PF00082	134 - 588	1.1e-48	Subtilase family
PA	PF02225	390 - 460	3.6e-12	PA domain
fn3_6	PF17766	662 - 762	7.7e-25	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001634)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G67360
fragaria_vesca	FvH4_1g17150 FvH4_2g33321 FvH4_2g33321 FvH4_2g33321 FvH4_2g33321 FvH4_2g33340
malus_domestica	MD08G1087900.v1.1 MD08G1088000.v1.1 MD15G1072700.v1.1 MD15G1292900.v1.1
prunus_persica	Prupe.1G425400_v2.0.a1 Prupe.1G425500_v2.0.a1 Prupe.1G425600_v2.0.a1 Prupe.1G425700_v2.0.a1 Prupe.6G224600_v2.0.a1
pyrus_communis	pycom08g07160 pycom15g06800 pycom15g25580
rosa_chinensis	RchiOBHm_Chr2g0107331 RchiOBHm_Chr6g0309241 RchiOBHm_Chr6g0309251
rosa_laevigata	RLG00000010577 RLG00000010578 RLG00000017570
rosa_multiflora	Rmu_co8090004.1_g000001 Rmu_co8331721.1_g000001 Rmu_sc0007504.1_g000002 Rmu_sc0015692.1_g000006
rosa_roxburghii	Rroxscaffold_2G00136430 Rroxscaffold_7G00159520 Rroxscaffold_7G00159530
rosa_rugosa	Rorug02G0142100 Rorug06G0370500 Rorug06G0370600
rosa_samantha	Rh2AG192800 Rh2BG204900 Rh2CG198000 Rh2DG200000 Rh6AG483900 Rh6AG484000 Rh6BG492900 Rh6BG493000 Rh6CG497800 Rh6CG497900 Rh6DG484400 Rh6DG484600
rosa_wichuraiana	Rw2G015270 Rw6G042130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	4 cut(s)	410, 431, 830, 1232
AatII	GACGTC	2 cut(s)	408, 1160
AccB1I	GGYRCC	2 cut(s)	1439, 1571
AccBSI	CCGCTC	3 cut(s)	722, 1662, 2022
AccI	GTMKAC	1 cut(s)	1599
AclI	AACGTT	1 cut(s)	837
AcsI	RAATTY	1 cut(s)	2011
AcvI	CACGTG	3 cut(s)	501, 1657, 1825
AcyI	GRCGYC	7 cut(s)	254, 405, 462, 1121, 1157, 1572, 1799
AfaI	GTAC	7 cut(s)	167, 320, 563, 968, 1862, 2054, 2093
AfiI	CCNNNNNNNGG	7 cut(s)	289, 388, 672, 726, 1435, 1837, 1849
AflII	CTTAAG	1 cut(s)	275
AflIII	ACRYGT	3 cut(s)	171, 500, 1822
AgeI	ACCGGT	1 cut(s)	1586
AgsI	TTSAA	5 cut(s)	316, 523, 1621, 1906, 2050
AhdI	GACNNNNNGTC	1 cut(s)	391
AjiI	CACGTC	2 cut(s)	1638, 1777
AjnI	CCWGG	4 cut(s)	557, 617, 743, 1540
AjuI	GAANNNNNNNTTGG	2 cut(s)	276, 308
AleI	CACNNNNGTG	1 cut(s)	2072
Alw21I	GWGCWC	4 cut(s)	104, 153, 327, 941
Alw26I	GTCTC	2 cut(s)	263, 624
Ama87I	CYCGRG	7 cut(s)	287, 308, 344, 1242, 1488, 2056, 2240
ApaI	GGGCCC	2 cut(s)	1690, 2081
ApeKI	GCWGC	4 cut(s)	247, 1345, 1675, 2226
ApoI	RAATTY	1 cut(s)	2011
AsiGI	ACCGGT	1 cut(s)	1586
AspLEI	GCGC	9 cut(s)	741, 743, 965, 1010, 1540, 1574, 1611, 1675, 1726
AsuHPI	GGTGA	6 cut(s)	1064, 1918, 2029, 2059, 2137, 2203
AvaI	CYCGRG	7 cut(s)	287, 308, 344, 1242, 1488, 2056, 2240
AvaII	GGWCC	7 cut(s)	473, 621, 675, 956, 1246, 1291, 1828
BaeGI	GKGCMC	5 cut(s)	500, 1016, 1442, 1690, 2081
BalI	TGGCCA	5 cut(s)	5, 439, 734, 806, 1781
BamHI	GGATCC	2 cut(s)	716, 1405
BanI	GGYRCC	2 cut(s)	1439, 1571
BanII	GRGCYC	4 cut(s)	327, 1690, 1706, 2081
BarI	GAAGNNNNNNTAC	2 cut(s)	2168, 2200
BauI	CACGAG	2 cut(s)	723, 934
BbrPI	CACGTG	3 cut(s)	501, 1657, 1825
BbsI	GAAGAC	1 cut(s)	2025
Bbv12I	GWGCWC	4 cut(s)	104, 153, 327, 941
BbvCI	CCTCAGC	1 cut(s)	1043
BbvI	GCAGC	4 cut(s)	259, 1332, 1662, 2238
BceAI	ACGGC	9 cut(s)	47, 250, 655, 1330, 1371, 1390, 1434, 1559, 1761
BcgI	CGANNNNNNTGC	6 cut(s)	119, 153, 1429, 1463, 1796, 1830
BciT130I	CCWGG	4 cut(s)	559, 619, 745, 1542
BciVI	GTATCC	1 cut(s)	698
BcoDI	GTCTC	2 cut(s)	263, 624
BfaI	CTAG	3 cut(s)	12, 87, 516
BfoI	RGCGCY	2 cut(s)	1575, 1727
BfrI	CTTAAG	1 cut(s)	275
BfuI	GTATCC	1 cut(s)	698
BglI	GCCNNNNNGGC	1 cut(s)	1745
BmcAI	AGTACT	1 cut(s)	2054
Bme18I	GGWCC	7 cut(s)	473, 621, 675, 956, 1246, 1291, 1828
BmeRI	GACNNNNNGTC	1 cut(s)	391
BmeT110I	CYCGRG	7 cut(s)	287, 308, 344, 1242, 1488, 2056, 2240
BmgBI	CACGTC	2 cut(s)	1638, 1777
BmsI	GCATC	1 cut(s)	195
BpiI	GAAGAC	1 cut(s)	2025
BpmI	CTGGAG	2 cut(s)	450, 1720
Bpu10I	CCTNAGC	1 cut(s)	1043
BsaAI	YACGTR	3 cut(s)	501, 1657, 1825
BsaBI	GATNNNNATC	3 cut(s)	929, 1410, 2282
BsaHI	GRCGYC	7 cut(s)	254, 405, 462, 1121, 1157, 1572, 1799
BsaWI	WCCGGW	3 cut(s)	1465, 1586, 1784
BsaXI	ACNNNNNCTCC	6 cut(s)	224, 254, 1613, 1643, 2132, 2162
Bsc4I	CCNNNNNNNGG	7 cut(s)	289, 388, 672, 726, 1435, 1837, 1849
Bse118I	RCCGGY	3 cut(s)	1136, 1348, 1586
Bse1I	ACTGG	5 cut(s)	167, 399, 433, 1023, 1703
Bse8I	GATNNNNATC	3 cut(s)	929, 1410, 2282
BseBI	CCWGG	4 cut(s)	559, 619, 745, 1542
BseGI	GGATG	3 cut(s)	228, 735, 2119
BseJI	GATNNNNATC	3 cut(s)	929, 1410, 2282
BseLI	CCNNNNNNNGG	7 cut(s)	289, 388, 672, 726, 1435, 1837, 1849
BseMII	CTCAG	2 cut(s)	1057, 1083
BseNI	ACTGG	5 cut(s)	167, 399, 433, 1023, 1703
BsePI	GCGCGC	1 cut(s)	739
BseRI	GAGGAG	2 cut(s)	275, 1334
BseSI	GKGCMC	5 cut(s)	500, 1016, 1442, 1690, 2081
BseX3I	CGGCCG	4 cut(s)	198, 942, 1419, 2082
BseXI	GCAGC	4 cut(s)	259, 1332, 1662, 2238
BseYI	CCCAGC	2 cut(s)	959, 1559
Bsh1285I	CGRYCG	7 cut(s)	201, 945, 1228, 1422, 1599, 1721, 2085
BshNI	GGYRCC	2 cut(s)	1439, 1571
BshTI	ACCGGT	1 cut(s)	1586
BsiEI	CGRYCG	7 cut(s)	201, 945, 1228, 1422, 1599, 1721, 2085
BsiHKAI	GWGCWC	4 cut(s)	104, 153, 327, 941
BsiHKCI	CYCGRG	7 cut(s)	287, 308, 344, 1242, 1488, 2056, 2240
BslFI	GGGAC	3 cut(s)	607, 896, 942
BslI	CCNNNNNNNGG	7 cut(s)	289, 388, 672, 726, 1435, 1837, 1849
BsmAI	GTCTC	2 cut(s)	263, 624
BsmBI	CGTCTC	2 cut(s)	263, 624
BsmFI	GGGAC	3 cut(s)	607, 896, 942
BsoBI	CYCGRG	7 cut(s)	287, 308, 344, 1242, 1488, 2056, 2240
Bsp120I	GGGCCC	2 cut(s)	1686, 2077
Bsp19I	CCATGG	1 cut(s)	2109
Bsp68I	TCGCGA	1 cut(s)	1026
BspCNI	CTCAG	2 cut(s)	1056, 1082
BspQI	GCTCTTC	1 cut(s)	383
BspT107I	GGYRCC	2 cut(s)	1439, 1571
BspTI	CTTAAG	1 cut(s)	275
BsrBI	CCGCTC	3 cut(s)	722, 1662, 2022
BsrFI	RCCGGY	3 cut(s)	1136, 1348, 1586
BsrI	ACTGG	5 cut(s)	167, 399, 433, 1023, 1703
BssAI	RCCGGY	3 cut(s)	1136, 1348, 1586
BssHII	GCGCGC	1 cut(s)	739
BssNI	GRCGYC	7 cut(s)	254, 405, 462, 1121, 1157, 1572, 1799
BssSI	CACGAG	2 cut(s)	723, 934
BssT1I	CCWWGG	2 cut(s)	1443, 2109
Bst2BI	CACGAG	2 cut(s)	723, 934
Bst2UI	CCWGG	4 cut(s)	559, 619, 745, 1542
Bst4CI	ACNGT	9 cut(s)	971, 1003, 1058, 1229, 1261, 1814, 1873, 2044, 2191
Bst6I	CTCTTC	2 cut(s)	383, 2174
BstACI	GRCGYC	7 cut(s)	254, 405, 462, 1121, 1157, 1572, 1799
BstAFI	CTTAAG	1 cut(s)	275
BstBAI	YACGTR	3 cut(s)	501, 1657, 1825
BstC8I	GCNNGC	7 cut(s)	138, 487, 694, 741, 1012, 1350, 1652
BstDEI	CTNAG	3 cut(s)	1043, 1069, 1555
BstDSI	CCRYGG	8 cut(s)	63, 232, 643, 666, 989, 1228, 1260, 2109
BstENI	CCTNNNNNAGG	1 cut(s)	1433
BstF5I	GGATG	3 cut(s)	228, 735, 2119
BstH2I	RGCGCY	2 cut(s)	1575, 1727
BstHHI	GCGC	9 cut(s)	741, 743, 965, 1010, 1540, 1574, 1611, 1675, 1726
BstMAI	GTCTC	2 cut(s)	263, 624
BstMCI	CGRYCG	7 cut(s)	201, 945, 1228, 1422, 1599, 1721, 2085
BstNI	CCWGG	4 cut(s)	559, 619, 745, 1542
BstSLI	GKGCMC	5 cut(s)	500, 1016, 1442, 1690, 2081
BstV1I	GCAGC	4 cut(s)	259, 1332, 1662, 2238
BstV2I	GAAGAC	1 cut(s)	2025
BstX2I	RGATCY	4 cut(s)	716, 924, 1405, 1984
BstXI	CCANNNNNNTGG	1 cut(s)	1591
BstYI	RGATCY	4 cut(s)	716, 924, 1405, 1984
BstZI	CGGCCG	4 cut(s)	198, 942, 1419, 2082
BsuI	GTATCC	1 cut(s)	698
BtgI	CCRYGG	8 cut(s)	63, 232, 643, 666, 989, 1228, 1260, 2109
BtgZI	GCGATG	2 cut(s)	926, 1392
BtrI	CACGTC	2 cut(s)	1638, 1777
BtsCI	GGATG	3 cut(s)	228, 735, 2119
BtsIMutI	CAGTG	4 cut(s)	160, 406, 426, 1016
BtuMI	TCGCGA	1 cut(s)	1026
Cac8I	GCNNGC	7 cut(s)	138, 487, 694, 741, 1012, 1350, 1652
CfoI	GCGC	9 cut(s)	741, 743, 965, 1010, 1540, 1574, 1611, 1675, 1726
Cfr10I	RCCGGY	3 cut(s)	1136, 1348, 1586
Cfr42I	CCGCGG	1 cut(s)	669
Cfr9I	CCCGGG	2 cut(s)	287, 1242
CseI	GACGC	8 cut(s)	162, 179, 262, 280, 470, 1129, 1343, 1807
Csp6I	GTAC	7 cut(s)	166, 319, 562, 967, 1861, 2053, 2092
CspAI	ACCGGT	1 cut(s)	1586
CviAII	CATG	6 cut(s)	7, 118, 847, 1174, 1288, 2110
CviQI	GTAC	7 cut(s)	166, 319, 562, 967, 1861, 2053, 2092
DdeI	CTNAG	3 cut(s)	1043, 1069, 1555
DinI	GGCGCC	1 cut(s)	1573
DrdI	GACNNNNNNGTC	4 cut(s)	410, 431, 830, 1232
DriI	GACNNNNNGTC	1 cut(s)	391
DseDI	GACNNNNNNGTC	4 cut(s)	410, 431, 830, 1232
EagI	CGGCCG	4 cut(s)	198, 942, 1419, 2082
Eam1104I	CTCTTC	2 cut(s)	383, 2174
Eam1105I	GACNNNNNGTC	1 cut(s)	391
EarI	CTCTTC	2 cut(s)	383, 2174
EciI	GGCGGA	4 cut(s)	83, 95, 281, 876
Ecl136II	GAGCTC	1 cut(s)	325
EclXI	CGGCCG	4 cut(s)	198, 942, 1419, 2082
Eco130I	CCWWGG	2 cut(s)	1443, 2109
Eco24I	GRGCYC	4 cut(s)	327, 1690, 1706, 2081
Eco47I	GGWCC	7 cut(s)	473, 621, 675, 956, 1246, 1291, 1828
Eco52I	CGGCCG	4 cut(s)	198, 942, 1419, 2082
Eco53kI	GAGCTC	1 cut(s)	325
Eco72I	CACGTG	3 cut(s)	501, 1657, 1825
Eco88I	CYCGRG	7 cut(s)	287, 308, 344, 1242, 1488, 2056, 2240
EcoICRI	GAGCTC	1 cut(s)	325
EcoNI	CCTNNNNNAGG	1 cut(s)	1433
EcoO109I	RGGNCCY	4 cut(s)	593, 621, 956, 1686
EcoRII	CCWGG	4 cut(s)	557, 617, 743, 1540
EcoT14I	CCWWGG	2 cut(s)	1443, 2109
EcoT38I	GRGCYC	4 cut(s)	327, 1690, 1706, 2081
EgeI	GGCGCC	1 cut(s)	1573
EheI	GGCGCC	1 cut(s)	1573
ErhI	CCWWGG	2 cut(s)	1443, 2109
Esp3I	CGTCTC	2 cut(s)	263, 624
FaeI	CATG	6 cut(s)	10, 121, 850, 1177, 1291, 2113
FaiI	YATR	6 cut(s)	8, 119, 848, 1175, 1289, 2111
FalI	AAGNNNNNCTT	2 cut(s)	911, 943
FaqI	GGGAC	3 cut(s)	607, 896, 942
FatI	CATG	6 cut(s)	6, 117, 846, 1173, 1287, 2109
FauI	CCCGC	5 cut(s)	664, 770, 946, 1003, 1659
FblI	GTMKAC	1 cut(s)	1599
FokI	GGATG	3 cut(s)	215, 742, 2126
FriOI	GRGCYC	4 cut(s)	327, 1690, 1706, 2081
FspBI	CTAG	3 cut(s)	12, 87, 516
GlaI	GCGC	9 cut(s)	740, 742, 964, 1009, 1539, 1573, 1610, 1674, 1725
GsaI	CCCAGC	2 cut(s)	963, 1563
GsuI	CTGGAG	2 cut(s)	450, 1720
HaeII	RGCGCY	2 cut(s)	1575, 1727
HgaI	GACGC	8 cut(s)	162, 179, 262, 280, 470, 1129, 1343, 1807
HhaI	GCGC	9 cut(s)	741, 743, 965, 1010, 1540, 1574, 1611, 1675, 1726
Hin1I	GRCGYC	7 cut(s)	254, 405, 462, 1121, 1157, 1572, 1799
Hin1II	CATG	6 cut(s)	10, 121, 850, 1177, 1291, 2113
Hin6I	GCGC	9 cut(s)	739, 741, 963, 1008, 1538, 1572, 1609, 1673, 1724
HinP1I	GCGC	9 cut(s)	739, 741, 963, 1008, 1538, 1572, 1609, 1673, 1724
HincII	GTYRAC	4 cut(s)	835, 1237, 1549, 1600
HindII	GTYRAC	4 cut(s)	835, 1237, 1549, 1600
HphI	GGTGA	6 cut(s)	1064, 1918, 2029, 2059, 2137, 2203
Hpy166II	GTNNAC	8 cut(s)	835, 1237, 1549, 1600, 1828, 1861, 1950, 2040
Hpy188III	TCNNGA	9 cut(s)	19, 308, 609, 799, 928, 1025, 1249, 1488, 2214
Hpy8I	GTNNAC	8 cut(s)	835, 1237, 1549, 1600, 1828, 1861, 1950, 2040
HpyAV	CCTTC	6 cut(s)	487, 913, 1198, 1470, 1813, 2007
HpyCH4III	ACNGT	9 cut(s)	971, 1003, 1058, 1229, 1261, 1814, 1873, 2044, 2191
HpyCH4V	TGCA	3 cut(s)	1114, 1173, 1771
HpyF3I	CTNAG	3 cut(s)	1043, 1069, 1555
Hsp92I	GRCGYC	7 cut(s)	254, 405, 462, 1121, 1157, 1572, 1799
Hsp92II	CATG	6 cut(s)	10, 121, 850, 1177, 1291, 2113
HspAI	GCGC	9 cut(s)	739, 741, 963, 1008, 1538, 1572, 1609, 1673, 1724
KasI	GGCGCC	1 cut(s)	1571
KflI	GGGWCCC	1 cut(s)	956
KroI	GCCGGC	1 cut(s)	1348
KroNI	GCCGGC	1 cut(s)	1350
KspI	CCGCGG	1 cut(s)	669
LguI	GCTCTTC	1 cut(s)	383
Lsp1109I	GCAGC	4 cut(s)	259, 1332, 1662, 2238
LweI	GCATC	1 cut(s)	195
MaeI	CTAG	3 cut(s)	12, 87, 516
MaeIII	GTNAC	9 cut(s)	401, 849, 1052, 1139, 1148, 1970, 2183, 2191, 2203
MbiI	CCGCTC	3 cut(s)	722, 1662, 2022
MboII	GAAGA	6 cut(s)	296, 370, 2025, 2140, 2188, 2191
MflI	RGATCY	4 cut(s)	716, 924, 1405, 1984
MlsI	TGGCCA	5 cut(s)	5, 439, 734, 806, 1781
MluCI	AATT	3 cut(s)	523, 1093, 2011
MluI	ACGCGT	1 cut(s)	171
MluNI	TGGCCA	5 cut(s)	5, 439, 734, 806, 1781
Mly113I	GGCGCC	1 cut(s)	1572
Mox20I	TGGCCA	5 cut(s)	5, 439, 734, 806, 1781
MroNI	GCCGGC	1 cut(s)	1348
MscI	TGGCCA	5 cut(s)	5, 439, 734, 806, 1781
MseI	TTAA	1 cut(s)	276
MslI	CAYNNNNRTG	3 cut(s)	800, 1640, 2072
Msp20I	TGGCCA	5 cut(s)	5, 439, 734, 806, 1781
MspA1I	CMGCKG	2 cut(s)	250, 668
MspCI	CTTAAG	1 cut(s)	275
MvaI	CCWGG	4 cut(s)	559, 619, 745, 1542
NaeI	GCCGGC	1 cut(s)	1350
NarI	GGCGCC	1 cut(s)	1572
NcoI	CCATGG	1 cut(s)	2109
NgoMIV	GCCGGC	1 cut(s)	1348
NlaIII	CATG	6 cut(s)	10, 121, 850, 1177, 1291, 2113
NmeAIII	GCCGAG	7 cut(s)	176, 226, 509, 834, 887, 920, 962
NmuCI	GTSAC	4 cut(s)	401, 849, 1052, 2191
NruI	TCGCGA	1 cut(s)	1026
OliI	CACNNNNGTG	1 cut(s)	2072
PaeR7I	CTCGAG	2 cut(s)	2056, 2240
PauI	GCGCGC	1 cut(s)	739
PciSI	GCTCTTC	1 cut(s)	383
PcsI	WCGNNNNNNNCGW	6 cut(s)	306, 828, 1380, 1830, 2033, 2096
PdiI	GCCGGC	1 cut(s)	1350
PfeI	GAWTC	4 cut(s)	1165, 1281, 1411, 1452
PflFI	GACNNNGTC	2 cut(s)	830, 1532
PfoI	TCCNGGA	2 cut(s)	712, 1160
PinAI	ACCGGT	1 cut(s)	1586
Ple19I	CGATCG	1 cut(s)	1721
PluTI	GGCGCC	1 cut(s)	1575
PmaCI	CACGTG	3 cut(s)	501, 1657, 1825
PmlI	CACGTG	3 cut(s)	501, 1657, 1825
Ppu21I	YACGTR	3 cut(s)	501, 1657, 1825
PpuMI	RGGWCCY	2 cut(s)	621, 956
Psp124BI	GAGCTC	1 cut(s)	327
Psp1406I	AACGTT	1 cut(s)	837
Psp5II	RGGWCCY	2 cut(s)	621, 956
Psp6I	CCWGG	4 cut(s)	557, 617, 743, 1540
PspCI	CACGTG	3 cut(s)	501, 1657, 1825
PspFI	CCCAGC	2 cut(s)	959, 1559
PspGI	CCWGG	4 cut(s)	557, 617, 743, 1540
PspOMI	GGGCCC	2 cut(s)	1686, 2077
PspPPI	RGGWCCY	2 cut(s)	621, 956
PspXI	VCTCGAGB	2 cut(s)	2056, 2240
PsuI	RGATCY	4 cut(s)	716, 924, 1405, 1984
PsyI	GACNNNGTC	2 cut(s)	830, 1532
PteI	GCGCGC	1 cut(s)	739
PvuI	CGATCG	1 cut(s)	1721
PvuII	CAGCTG	1 cut(s)	250
RruI	TCGCGA	1 cut(s)	1026
RsaI	GTAC	7 cut(s)	167, 320, 563, 968, 1862, 2054, 2093
RsaNI	GTAC	7 cut(s)	166, 319, 562, 967, 1861, 2053, 2092
RseI	CAYNNNNRTG	3 cut(s)	800, 1640, 2072
SacI	GAGCTC	1 cut(s)	327
SacII	CCGCGG	1 cut(s)	669
SalI	GTCGAC	1 cut(s)	1598
SapI	GCTCTTC	1 cut(s)	383
SaqAI	TTAA	1 cut(s)	276
ScaI	AGTACT	1 cut(s)	2054
SfaNI	GCATC	1 cut(s)	195
SfoI	GGCGCC	1 cut(s)	1573
Sfr274I	CTCGAG	2 cut(s)	2056, 2240
Sfr303I	CCGCGG	1 cut(s)	669
SgrBI	CCGCGG	1 cut(s)	669
SinI	GGWCC	7 cut(s)	473, 621, 675, 956, 1246, 1291, 1828
SlaI	CTCGAG	2 cut(s)	2056, 2240
SmaI	CCCGGG	2 cut(s)	289, 1244
SmiMI	CAYNNNNRTG	3 cut(s)	800, 1640, 2072
SmlI	CTYRAG	3 cut(s)	275, 2056, 2240
SmoI	CTYRAG	3 cut(s)	275, 2056, 2240
Sse9I	AATT	3 cut(s)	523, 1093, 2011
SspDI	GGCGCC	1 cut(s)	1571
SspMI	CTAG	3 cut(s)	12, 87, 516
SstI	GAGCTC	1 cut(s)	327
StyI	CCWWGG	2 cut(s)	1443, 2109
TaaI	ACNGT	9 cut(s)	971, 1003, 1058, 1229, 1261, 1814, 1873, 2044, 2191
TasI	AATT	3 cut(s)	523, 1093, 2011
TatI	WGTACW	1 cut(s)	2052
TfiI	GAWTC	4 cut(s)	1165, 1281, 1411, 1452
Tru1I	TTAA	1 cut(s)	276
Tru9I	TTAA	1 cut(s)	276
TscAI	CASTG	4 cut(s)	167, 406, 433, 1023
TseFI	GTSAC	4 cut(s)	401, 849, 1052, 2191
TseI	GCWGC	4 cut(s)	247, 1345, 1675, 2226
Tsp45I	GTSAC	4 cut(s)	401, 849, 1052, 2191
TspGWI	ACGGA	3 cut(s)	660, 667, 978
TspMI	CCCGGG	2 cut(s)	287, 1242
TspRI	CASTG	4 cut(s)	167, 406, 433, 1023
Tth111I	GACNNNGTC	2 cut(s)	830, 1532
Vha464I	CTTAAG	1 cut(s)	275
VpaK11BI	GGWCC	7 cut(s)	473, 621, 675, 956, 1246, 1291, 1828
XagI	CCTNNNNNAGG	1 cut(s)	1433
XapI	RAATTY	1 cut(s)	2011
XhoI	CTCGAG	2 cut(s)	2056, 2240
XmaI	CCCGGG	2 cut(s)	287, 1242
XmiI	GTMKAC	1 cut(s)	1599
XspI	CTAG	3 cut(s)	12, 87, 516
ZraI	GACGTC	2 cut(s)	406, 1158
ZrmI	AGTACT	1 cut(s)	2054

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.