Prupe.1G425500_v2.0.a1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001634
Physical Location & Seq
Forward (+)
36626426 .. 36630116
3691 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G425500.1

Sequence Viewer

Length: 2340 bp
ATGAAAACATCCATTTGCATGCTTTCACTAACGCGGATGCTGATGCTTCTGGTTCTCTCTCACAACATCTTGTGCACGGTAGCGGTAGCAGGAGAGAGAGAGCAGCAGACGAAAAAGACTTACATCATTCAGATGGACAAATCCAAAATGCCTGCAAGTTTTGCCGACGATCATTTCCAATGGTATGGCTCCTCTTTGAAATCGGTGTCCAACTCAGCAGACATGCTCTACACCTACAAAAATGTTATCCACGGCTTCTCTACCAGGCTAACTGCTGAGGAAGCTGAGCTTCTTGAAAGACAATCCGGAATCCTTTCTGTTATGCCTGAGTTGAGATATGAGCTCCACACAACTCGGACGCCCCAGTTCCTTGGAATGTTAGGCGGCATAAACGAAGCCGTCTTCCCGGCATCAGAGAAACTCGGCAAGGTGATTATTGGAGTGGTTGACACCGGCGTATGGCCTGAGATCAAAAGCTACGATGACAAAGGGCTTGGGCCAGTGCCAAGAAGCTGGAGAGGGCAGTGTGAGGAAGGCAAGAACTTCAACTCCTCAAGCTGCAACCGAAAACTCATTGGTGCGAGGTTTTTCCCAAAAGGGTATGAAGCATCAAATCTTGGACCCATTGATGAAAAAGTAGAATCAAGATCACCAAGAGATGTTGATGGCCATGGAACTCAAGCCTCAACCATTGCAGCCGGGTCTGCAGTTCCAGGAGCTAGCCTCTATGGTTATGCTTCGGGGACAGCAAGGGGAATGGCTACACAAGCTAGAGTGGCCACATATAAGGCTTGCTGGTCTGGTTGGTGCTTGAGCTCTGATATTCTAGCCGCAATGGACAAAGCTGTTGAAGATGGTGTCCACATTTTATCTGTGTCTATTGGGAGATCGCAGTATGAGGATTTCTACACCGACTTTATTGCTATTGGAGCATTCTCTGCCATGGCCAAGGGAGTCTTTGTATCGTGTTCAGCTGGAAATCGTGGGCCAGAAGCGGATAGCACTTCTAACAACGCACCTTGGATAACCACCGTGGGTGCTGGAACAATAGACCGTGATTTCCCCGCTCATGTTAGCTTGGGAAATGGGAAAAAATACAGAGGAGCATCAATTTACAGCGGAACACACTTATCTAGTGGATTGCATCCGCTTGTTTATGCTCGCAATGCAAGTAACTCCACAAGTGACTCCACAAGTGATCCATGCGCTCCTGACAGCTTGATTCCTGAAAAGGTTTTTGGGAAAATTGTGGTGTGTGATCAGGGAGGAACTTACAGCAGGGTCGATAAGAGTATGGTGGTCAAAAAGGCCGGTGGCATGGGGATGATTTTGGCTGACATTGAAGGTTATGGAGAAGAACTTGTTGTCGACTCATATGTCTTGCCTGTAGTAGTGGTAGGCCAAAAAGAAGGTGATGCAATAAAAAGGTATATTGTCTCCCACGACAATCCAAAAGCCACATTTTCTGGCGGGAAGACAGAGTTAGGTGTCGAACCCTCACCGGTGGTGGCAGCATTCAGCTCTCGAGGACCAAATCCAGTCGCTTTAACAGTACTCAAACCAGACCTAATAGCACCAGGAGTGAATATCCTAGCTGGGTGGACTGGTGCACTTGGACCAGCTAGACGAGCTGAGGACACCAGGCGGGTGAGCTTCAACATTTTTTCAGGTACATCCATGTCATGCCCACATGTGAGTGGACTGGCTGCAGTTCTCAAGGCCGCTCACCCCAAATGGAGCCCTGCAGCCATTAAGTCTGCTCTCATGACCACATCCTATGCAACGTACAAAAATGGTGCACCAATCAAAGATGTTGCTACTGGAAAACCTGCAACACCATTTGATTATGGCGCCGGGCATGTCGACCCTGTGGCAGCCCTTGACCCAGGCCTTGTTTATGATCTTGGTGTCAAGGACTACCTAAACTTCCTCTGTGCCTATCGTTATACAAGCAGCGATATCAAGATACTCACCCACATAGACTTCACCTGCGATTCAAGCAAGAATTACAGCGCTGGAGATTTAAATTATCCATCTTTTGCTGTTTCCCTAAACACAAATTCAGGCAACTGGGGAGCAGGCACTAAAATATACACAAGGACTCTCACCAATGTTGGTACCCCAGGAACATACAAGGTATCTGTGTCTACACCATCACCAGCTGTGAAGATCTTGGTTGAACCAAAATCACTGAGTTTCACTCGAGCATATGAGAAGAAGACTTATACTGTGACTTTTGTGGTCAGTGCTATGCCATCTGGTACAAACAACTTTACTCGCTTGGAATGGTCTGATGGGAAACACATAGTGAGTAGTCCTATTGCTGTCAGCTGGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

780

Amino Acids

83.32

Weight (kDa)

8.48

Isoelectric Point (pI)

33.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1997
AasI GACNNNNNNGTC 1 cut(s) 1376
Acc36I ACCTGC 2 cut(s) 1837, 1997
Acc65I GGTACC 1 cut(s) 2117
AccB1I GGYRCC 2 cut(s) 1850, 2117
AccBSI CCGCTC 2 cut(s) 1067, 1724
AccI GTMKAC 3 cut(s) 1368, 1863, 2147
AccII CGCG 1 cut(s) 34
AccIII TCCGGA 1 cut(s) 305
AclWI GGATC 1 cut(s) 1193
AcoI YGGCCR 3 cut(s) 667, 777, 945
AcsI RAATTY 1 cut(s) 2059
AcyI GRCGYC 2 cut(s) 359, 1851
AdeI CACNNNGTG 1 cut(s) 2308
AfaI GTAC 5 cut(s) 1554, 1672, 1787, 2119, 2263
AfeI AGCGCT 1 cut(s) 2014
AfiI CCNNNNNNNGG 1 cut(s) 459
AflIII ACRYGT 1 cut(s) 1690
AgeI ACCGGT 1 cut(s) 1501
AgsI TTSAA 8 cut(s) 199, 296, 547, 851, 1343, 1657, 1998, 2180
AjnI CCWGG 6 cut(s) 263, 712, 1576, 1640, 1885, 2122
AjuI GAANNNNNNNTTGG 2 cut(s) 1221, 1253
AloI GAACNNNNNNTCC 2 cut(s) 533, 565
Alw21I GWGCWC 5 cut(s) 77, 345, 818, 1612, 1801
Alw26I GTCTC 1 cut(s) 1441
Alw44I GTGCAC 3 cut(s) 73, 1608, 1797
AlwI GGATC 1 cut(s) 1193
Ama87I CYCGRG 2 cut(s) 1524, 2202
Aor13HI TCCGGA 1 cut(s) 305
Aor51HI AGCGCT 1 cut(s) 2014
ApaLI GTGCAC 3 cut(s) 73, 1608, 1797
ApeKI GCWGC 8 cut(s) 103, 558, 695, 1511, 1706, 1745, 1874, 1953
ApoI RAATTY 1 cut(s) 2059
AsiGI ACCGGT 1 cut(s) 1501
Asp700I GAANNNNTTC 1 cut(s) 313
Asp718I GGTACC 1 cut(s) 2117
AspLEI GCGC 3 cut(s) 1208, 1853, 2015
AspS9I GGNCC 5 cut(s) 497, 620, 986, 1529, 1616
AsuC2I CCSGG 3 cut(s) 407, 700, 1855
AsuNHI GCTAGC 1 cut(s) 719
AvaI CYCGRG 2 cut(s) 1524, 2202
AvaII GGWCC 3 cut(s) 620, 1529, 1616
BaeGI GKGCMC 3 cut(s) 77, 1612, 1801
BalI TGGCCA 3 cut(s) 669, 779, 947
BanI GGYRCC 2 cut(s) 1850, 2117
BanII GRGCYC 3 cut(s) 345, 818, 1742
BbsI GAAGAC 3 cut(s) 394, 1481, 2225
Bbv12I GWGCWC 5 cut(s) 77, 345, 818, 1612, 1801
BbvCI CCTCAGC 2 cut(s) 276, 1632
BbvI GCAGC 8 cut(s) 115, 545, 707, 1523, 1693, 1757, 1886, 1965
BccI CCATC 7 cut(s) 127, 659, 848, 2041, 2161, 2263, 2288
BceAI ACGGC 2 cut(s) 268, 383
BcgI CGANNNNNNTGC 2 cut(s) 902, 936
BciT130I CCWGG 6 cut(s) 265, 714, 1578, 1642, 1887, 2124
BclI TGATCA 1 cut(s) 1258
BcnI CCSGG 3 cut(s) 407, 700, 1855
BcoDI GTCTC 1 cut(s) 1441
BfaI CTAG 6 cut(s) 720, 771, 827, 1134, 1592, 1623
BfmI CTRYAG 4 cut(s) 705, 1386, 1707, 1743
BfoI RGCGCY 2 cut(s) 1854, 2016
BfuAI ACCTGC 2 cut(s) 1837, 1997
BglII AGATCT 1 cut(s) 2169
BlpI GCTNAGC 1 cut(s) 285
BmcAI AGTACT 1 cut(s) 1554
Bme1390I CCNGG 9 cut(s) 265, 407, 700, 714, 1578, 1642, 1855, 1887, 2124
Bme18I GGWCC 3 cut(s) 620, 1529, 1616
BmeT110I CYCGRG 2 cut(s) 1524, 2202
BmgT120I GGNCC 5 cut(s) 497, 620, 986, 1529, 1616
BmiI GGNNCC 5 cut(s) 190, 622, 1739, 1852, 2119
BmrFI CCNGG 9 cut(s) 265, 407, 700, 714, 1578, 1642, 1855, 1887, 2124
BmrI ACTGGG 2 cut(s) 358, 2080
BmsI GCATC 7 cut(s) 27, 33, 419, 617, 1115, 1153, 1405
BmtI GCTAGC 1 cut(s) 723
BmuI ACTGGG 2 cut(s) 358, 2080
BpiI GAAGAC 3 cut(s) 394, 1481, 2225
BplI GAGNNNNNCTC 4 cut(s) 708, 740, 2185, 2217
BpmI CTGGAG 2 cut(s) 535, 2037
Bpu10I CCTNAGC 2 cut(s) 276, 1632
Bpu1102I GCTNAGC 1 cut(s) 285
BpuEI CTTGAG 4 cut(s) 538, 663, 832, 1700
BpuMI CCSGG 3 cut(s) 407, 700, 1855
BsaHI GRCGYC 2 cut(s) 359, 1851
BsaJI CCNNGG 9 cut(s) 250, 370, 670, 942, 948, 1019, 1032, 1885, 2122
BsaWI WCCGGW 2 cut(s) 305, 1501
BsaXI ACNNNNNCTCC 4 cut(s) 533, 563, 945, 975
Bsc4I CCNNNNNNNGG 1 cut(s) 459
Bse118I RCCGGY 3 cut(s) 452, 1310, 1501
Bse1I ACTGG 7 cut(s) 364, 500, 1538, 1609, 1707, 1825, 2075
Bse3DI GCAATG 3 cut(s) 690, 840, 1171
BseAI TCCGGA 1 cut(s) 305
BseBI CCWGG 6 cut(s) 265, 714, 1578, 1642, 1887, 2124
BseDI CCNNGG 9 cut(s) 250, 370, 670, 942, 948, 1019, 1032, 1885, 2122
BseGI GGATG 6 cut(s) 8, 42, 1144, 1329, 1673, 1772
BseLI CCNNNNNNNGG 1 cut(s) 459
BseMI GCAATG 3 cut(s) 690, 840, 1171
BseMII CTCAG 7 cut(s) 228, 267, 276, 318, 456, 1623, 2183
BseNI ACTGG 7 cut(s) 364, 500, 1538, 1609, 1707, 1825, 2075
BseRI GAGGAG 3 cut(s) 181, 541, 1116
BseSI GKGCMC 3 cut(s) 77, 1612, 1801
BseXI GCAGC 8 cut(s) 115, 545, 707, 1523, 1693, 1757, 1886, 1965
BseYI CCCAGC 1 cut(s) 1595
Bsh1236I CGCG 1 cut(s) 34
BshNI GGYRCC 2 cut(s) 1850, 2117
BshTI ACCGGT 1 cut(s) 1501
BsiHKAI GWGCWC 5 cut(s) 77, 345, 818, 1612, 1801
BsiHKCI CYCGRG 2 cut(s) 1524, 2202
BsiSI CCGG 7 cut(s) 306, 407, 453, 699, 1311, 1502, 1854
BslFI GGGAC 1 cut(s) 757
BslI CCNNNNNNNGG 1 cut(s) 459
BsmAI GTCTC 1 cut(s) 1441
BsmFI GGGAC 1 cut(s) 757
BsmI GAATGC 2 cut(s) 932, 1514
BsoBI CYCGRG 2 cut(s) 1524, 2202
Bsp1286I GDGCHC 6 cut(s) 77, 345, 818, 1612, 1742, 1801
Bsp13I TCCGGA 1 cut(s) 305
Bsp143I GATC 8 cut(s) 169, 468, 647, 887, 1198, 1258, 1900, 2169
Bsp1720I GCTNAGC 1 cut(s) 285
Bsp19I CCATGG 2 cut(s) 670, 942
BspCNI CTCAG 7 cut(s) 227, 268, 277, 319, 457, 1624, 2184
BspEI TCCGGA 1 cut(s) 305
BspFNI CGCG 1 cut(s) 34
BspHI TCATGA 1 cut(s) 1764
BspLI GGNNCC 5 cut(s) 190, 622, 1739, 1852, 2119
BspMAI CTGCAG 3 cut(s) 709, 1711, 1747
BspMI ACCTGC 2 cut(s) 1837, 1997
BspOI GCTAGC 1 cut(s) 723
BspPI GGATC 1 cut(s) 1193
BspT107I GGYRCC 2 cut(s) 1850, 2117
BsrBI CCGCTC 2 cut(s) 1067, 1724
BsrDI GCAATG 3 cut(s) 690, 840, 1171
BsrFI RCCGGY 3 cut(s) 452, 1310, 1501
BsrI ACTGG 7 cut(s) 364, 500, 1538, 1609, 1707, 1825, 2075
BssAI RCCGGY 3 cut(s) 452, 1310, 1501
BssECI CCNNGG 9 cut(s) 250, 370, 670, 942, 948, 1019, 1032, 1885, 2122
BssMI GATC 8 cut(s) 169, 468, 647, 887, 1198, 1258, 1900, 2169
BssNI GRCGYC 2 cut(s) 359, 1851
BssT1I CCWWGG 5 cut(s) 370, 670, 942, 948, 1019
Bst2UI CCWGG 6 cut(s) 265, 714, 1578, 1642, 1887, 2124
Bst4CI ACNGT 5 cut(s) 79, 1033, 1055, 1552, 2230
BstACI GRCGYC 2 cut(s) 359, 1851
BstAPI GCANNNNNTGC 2 cut(s) 161, 938
BstC8I GCNNGC 6 cut(s) 20, 153, 721, 793, 1162, 2080
BstDEI CTNAG 7 cut(s) 214, 276, 285, 327, 465, 1632, 2192
BstDSI CCRYGG 4 cut(s) 250, 670, 942, 1032
BstF5I GGATG 6 cut(s) 8, 42, 1144, 1329, 1673, 1772
BstFNI CGCG 1 cut(s) 34
BstH2I RGCGCY 2 cut(s) 1854, 2016
BstHHI GCGC 3 cut(s) 1208, 1853, 2015
BstKTI GATC 8 cut(s) 172, 471, 650, 890, 1201, 1261, 1903, 2172
BstMAI GTCTC 1 cut(s) 1441
BstMBI GATC 8 cut(s) 169, 468, 647, 887, 1198, 1258, 1900, 2169
BstNI CCWGG 6 cut(s) 265, 714, 1578, 1642, 1887, 2124
BstNSI RCATGY 4 cut(s) 22, 226, 1694, 1862
BstSCI CCNGG 9 cut(s) 263, 405, 698, 712, 1576, 1640, 1853, 1885, 2122
BstSFI CTRYAG 4 cut(s) 705, 1386, 1707, 1743
BstSLI GKGCMC 3 cut(s) 77, 1612, 1801
BstUI CGCG 1 cut(s) 34
BstV1I GCAGC 8 cut(s) 115, 545, 707, 1523, 1693, 1757, 1886, 1965
BstV2I GAAGAC 3 cut(s) 394, 1481, 2225
BstX2I RGATCY 1 cut(s) 2169
BstXI CCANNNNNNTGG 3 cut(s) 185, 371, 513
BstYI RGATCY 1 cut(s) 2169
BtgI CCRYGG 4 cut(s) 250, 670, 942, 1032
BtsCI GGATG 6 cut(s) 8, 42, 1144, 1329, 1673, 1772
BtsI GCAGTG 1 cut(s) 530
BtsIMutI CAGTG 4 cut(s) 507, 530, 2189, 2251
BveI ACCTGC 2 cut(s) 1837, 1997
Cac8I GCNNGC 6 cut(s) 20, 153, 721, 793, 1162, 2080
CciI TCATGA 1 cut(s) 1764
CfoI GCGC 3 cut(s) 1208, 1853, 2015
Cfr10I RCCGGY 3 cut(s) 452, 1310, 1501
Cfr13I GGNCC 5 cut(s) 497, 620, 986, 1529, 1616
CseI GACGC 1 cut(s) 367
Csp6I GTAC 5 cut(s) 1553, 1671, 1786, 2118, 2262
CspAI ACCGGT 1 cut(s) 1501
CviQI GTAC 5 cut(s) 1553, 1671, 1786, 2118, 2262
DdeI CTNAG 7 cut(s) 214, 276, 285, 327, 465, 1632, 2192
DinI GGCGCC 1 cut(s) 1852
DpnI GATC 8 cut(s) 171, 470, 649, 889, 1200, 1260, 1902, 2171
DpnII GATC 8 cut(s) 169, 468, 647, 887, 1198, 1258, 1900, 2169
DraI TTTAAA 1 cut(s) 2025
DraIII CACNNNGTG 1 cut(s) 2308
DrdI GACNNNNNNGTC 1 cut(s) 1376
DseDI GACNNNNNNGTC 1 cut(s) 1376
EaeI YGGCCR 3 cut(s) 667, 777, 945
Ecl136II GAGCTC 2 cut(s) 343, 816
Eco130I CCWWGG 5 cut(s) 370, 670, 942, 948, 1019
Eco147I AGGCCT 1 cut(s) 1890
Eco24I GRGCYC 3 cut(s) 345, 818, 1742
Eco32I GATATC 1 cut(s) 1960
Eco47I GGWCC 3 cut(s) 620, 1529, 1616
Eco47III AGCGCT 1 cut(s) 2014
Eco53kI GAGCTC 2 cut(s) 343, 816
Eco88I CYCGRG 2 cut(s) 1524, 2202
EcoICRI GAGCTC 2 cut(s) 343, 816
EcoRII CCWGG 6 cut(s) 263, 712, 1576, 1640, 1885, 2122
EcoRV GATATC 1 cut(s) 1960
EcoT14I CCWWGG 5 cut(s) 370, 670, 942, 948, 1019
EcoT38I GRGCYC 3 cut(s) 345, 818, 1742
EgeI GGCGCC 1 cut(s) 1852
EheI GGCGCC 1 cut(s) 1852
ErhI CCWWGG 5 cut(s) 370, 670, 942, 948, 1019
FalI AAGNNNNNCTT 4 cut(s) 273, 305, 941, 973
FaqI GGGAC 1 cut(s) 757
FauI CCCGC 3 cut(s) 1072, 1463, 1638
FauNDI CATATG 2 cut(s) 1375, 2209
FbaI TGATCA 1 cut(s) 1258
FblI GTMKAC 3 cut(s) 1368, 1863, 2147
FokI GGATG 5 cut(s) 49, 1131, 1336, 1660, 1759
FriOI GRGCYC 3 cut(s) 345, 818, 1742
FspBI CTAG 6 cut(s) 720, 771, 827, 1134, 1592, 1623
GlaI GCGC 3 cut(s) 1207, 1852, 2014
GsaI CCCAGC 1 cut(s) 1599
GsuI CTGGAG 2 cut(s) 535, 2037
HaeII RGCGCY 2 cut(s) 1854, 2016
HapII CCGG 7 cut(s) 306, 407, 453, 699, 1311, 1502, 1854
HgaI GACGC 1 cut(s) 367
HhaI GCGC 3 cut(s) 1208, 1853, 2015
Hin1I GRCGYC 2 cut(s) 359, 1851
Hin6I GCGC 3 cut(s) 1206, 1851, 2013
HinP1I GCGC 3 cut(s) 1206, 1851, 2013
HincII GTYRAC 3 cut(s) 448, 1369, 1864
HindII GTYRAC 3 cut(s) 448, 1369, 1864
HinfI GANTC 8 cut(s) 309, 641, 954, 1187, 1222, 1370, 1994, 2101
HpaII CCGG 7 cut(s) 306, 407, 453, 699, 1311, 1502, 1854
Hpy188I TCNGA 5 cut(s) 132, 357, 415, 820, 2293
Hpy188III TCNNGA 8 cut(s) 293, 306, 645, 1211, 1226, 1524, 1765, 1963
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 3 cut(s) 527, 1337, 1403
HpyCH4III ACNGT 5 cut(s) 79, 1033, 1055, 1552, 2230
HpyCH4IV ACGT 1 cut(s) 1784
HpyF3I CTNAG 7 cut(s) 214, 276, 285, 327, 465, 1632, 2192
HpySE526I ACGT 1 cut(s) 1784
Hsp92I GRCGYC 2 cut(s) 359, 1851
HspAI GCGC 3 cut(s) 1206, 1851, 2013
KasI GGCGCC 1 cut(s) 1850
Kpn2I TCCGGA 1 cut(s) 305
KpnI GGTACC 1 cut(s) 2121
Ksp22I TGATCA 1 cut(s) 1258
Kzo9I GATC 8 cut(s) 169, 468, 647, 887, 1198, 1258, 1900, 2169
LmnI GCTCC 8 cut(s) 194, 348, 716, 929, 1103, 1213, 1737, 2075
Lsp1109I GCAGC 8 cut(s) 115, 545, 707, 1523, 1693, 1757, 1886, 1965
LweI GCATC 7 cut(s) 27, 33, 419, 617, 1115, 1153, 1405
MaeI CTAG 6 cut(s) 720, 771, 827, 1134, 1592, 1623
MaeII ACGT 1 cut(s) 1784
MaeIII GTNAC 3 cut(s) 1172, 1184, 2230
MalI GATC 8 cut(s) 171, 470, 649, 889, 1200, 1260, 1902, 2171
MbiI CCGCTC 2 cut(s) 1067, 1724
MboI GATC 8 cut(s) 169, 468, 647, 887, 1198, 1258, 1900, 2169
MboII GAAGA 7 cut(s) 394, 863, 1367, 1486, 2179, 2227, 2230
MflI RGATCY 1 cut(s) 2169
MhlI GDGCHC 6 cut(s) 77, 345, 818, 1612, 1742, 1801
MlsI TGGCCA 3 cut(s) 669, 779, 947
MluCI AATT 5 cut(s) 1110, 1245, 2005, 2026, 2059
MluNI TGGCCA 3 cut(s) 669, 779, 947
Mly113I GGCGCC 1 cut(s) 1851
MlyI GAGTC 4 cut(s) 963, 1181, 1364, 2095
MmeI TCCRAC 1 cut(s) 234
Mox20I TGGCCA 3 cut(s) 669, 779, 947
MroI TCCGGA 1 cut(s) 305
MroXI GAANNNNTTC 1 cut(s) 313
MscI TGGCCA 3 cut(s) 669, 779, 947
MseI TTAA 4 cut(s) 1547, 1752, 2024, 2338
MslI CAYNNNNRTG 4 cut(s) 17, 131, 1322, 1695
Msp20I TGGCCA 3 cut(s) 669, 779, 947
MspA1I CMGCKG 4 cut(s) 974, 1119, 2162, 2331
MspI CCGG 7 cut(s) 306, 407, 453, 699, 1311, 1502, 1854
MspR9I CCNGG 9 cut(s) 265, 407, 700, 714, 1578, 1642, 1855, 1887, 2124
Mva1269I GAATGC 2 cut(s) 932, 1514
MvaI CCWGG 6 cut(s) 265, 714, 1578, 1642, 1887, 2124
MvnI CGCG 1 cut(s) 34
NarI GGCGCC 1 cut(s) 1851
NciI CCSGG 3 cut(s) 407, 700, 1855
NcoI CCATGG 2 cut(s) 670, 942
NdeI CATATG 2 cut(s) 1375, 2209
NdeII GATC 8 cut(s) 169, 468, 647, 887, 1198, 1258, 1900, 2169
NheI GCTAGC 1 cut(s) 719
NlaIV GGNNCC 5 cut(s) 190, 622, 1739, 1852, 2119
NmeAIII GCCGAG 1 cut(s) 402
NmuCI GTSAC 2 cut(s) 1184, 2230
NspI RCATGY 4 cut(s) 22, 226, 1694, 1862
PaeI GCATGC 1 cut(s) 22
PaeR7I CTCGAG 2 cut(s) 1524, 2202
PagI TCATGA 1 cut(s) 1764
PaqCI CACCTGC 1 cut(s) 1997
PceI AGGCCT 1 cut(s) 1890
PciI ACATGT 1 cut(s) 1690
PctI GAATGC 2 cut(s) 932, 1514
PdmI GAANNNNTTC 1 cut(s) 313
PfeI GAWTC 4 cut(s) 309, 641, 1222, 1994
PfoI TCCNGGA 1 cut(s) 712
PinAI ACCGGT 1 cut(s) 1501
PleI GAGTC 4 cut(s) 962, 1181, 1364, 2095
PluTI GGCGCC 1 cut(s) 1854
PpsI GAGTC 4 cut(s) 962, 1181, 1364, 2095
PscI ACATGT 1 cut(s) 1690
Psp124BI GAGCTC 2 cut(s) 345, 818
Psp6I CCWGG 6 cut(s) 263, 712, 1576, 1640, 1885, 2122
PspFI CCCAGC 1 cut(s) 1595
PspGI CCWGG 6 cut(s) 263, 712, 1576, 1640, 1885, 2122
PspN4I GGNNCC 5 cut(s) 190, 622, 1739, 1852, 2119
PspPI GGNCC 5 cut(s) 497, 620, 986, 1529, 1616
PspXI VCTCGAGB 1 cut(s) 2202
PstI CTGCAG 3 cut(s) 709, 1711, 1747
PsuI RGATCY 1 cut(s) 2169
PvuII CAGCTG 3 cut(s) 974, 2162, 2331
RsaI GTAC 5 cut(s) 1554, 1672, 1787, 2119, 2263
RsaNI GTAC 5 cut(s) 1553, 1671, 1786, 2118, 2262
RseI CAYNNNNRTG 4 cut(s) 17, 131, 1322, 1695
SacI GAGCTC 2 cut(s) 345, 818
SalI GTCGAC 2 cut(s) 1367, 1862
SaqAI TTAA 4 cut(s) 1547, 1752, 2024, 2338
Sau3AI GATC 8 cut(s) 169, 468, 647, 887, 1198, 1258, 1900, 2169
Sau96I GGNCC 5 cut(s) 497, 620, 986, 1529, 1616
ScaI AGTACT 1 cut(s) 1554
SchI GAGTC 4 cut(s) 963, 1181, 1364, 2095
ScrFI CCNGG 9 cut(s) 265, 407, 700, 714, 1578, 1642, 1855, 1887, 2124
SduI GDGCHC 6 cut(s) 77, 345, 818, 1612, 1742, 1801
SfaNI GCATC 7 cut(s) 27, 33, 419, 617, 1115, 1153, 1405
SfcI CTRYAG 4 cut(s) 705, 1386, 1707, 1743
SfoI GGCGCC 1 cut(s) 1852
Sfr274I CTCGAG 2 cut(s) 1524, 2202
SgrAI CRCCGGYG 2 cut(s) 452, 1501
SinI GGWCC 3 cut(s) 620, 1529, 1616
SlaI CTCGAG 2 cut(s) 1524, 2202
SmiI ATTTAAAT 1 cut(s) 2025
SmiMI CAYNNNNRTG 4 cut(s) 17, 131, 1322, 1695
SmlI CTYRAG 6 cut(s) 553, 678, 811, 1524, 1715, 2202
SmoI CTYRAG 6 cut(s) 553, 678, 811, 1524, 1715, 2202
SphI GCATGC 1 cut(s) 22
Sse9I AATT 5 cut(s) 1110, 1245, 2005, 2026, 2059
SseBI AGGCCT 1 cut(s) 1890
SspDI GGCGCC 1 cut(s) 1850
SspMI CTAG 6 cut(s) 720, 771, 827, 1134, 1592, 1623
SstI GAGCTC 2 cut(s) 345, 818
StuI AGGCCT 1 cut(s) 1890
StyD4I CCNGG 9 cut(s) 263, 405, 698, 712, 1576, 1640, 1853, 1885, 2122
StyI CCWWGG 5 cut(s) 370, 670, 942, 948, 1019
SwaI ATTTAAAT 1 cut(s) 2025
TaaI ACNGT 5 cut(s) 79, 1033, 1055, 1552, 2230
TaiI ACGT 1 cut(s) 1787
TaqI TCGA 6 cut(s) 1284, 1368, 1491, 1525, 1863, 2203
TasI AATT 5 cut(s) 1110, 1245, 2005, 2026, 2059
TatI WGTACW 1 cut(s) 1552
TauI GCSGC 3 cut(s) 387, 833, 1724
TfiI GAWTC 4 cut(s) 309, 641, 1222, 1994
Tru1I TTAA 4 cut(s) 1547, 1752, 2024, 2338
Tru9I TTAA 4 cut(s) 1547, 1752, 2024, 2338
TscAI CASTG 4 cut(s) 507, 530, 2196, 2251
TseFI GTSAC 2 cut(s) 1184, 2230
TseI GCWGC 8 cut(s) 103, 558, 695, 1511, 1706, 1745, 1874, 1953
Tsp45I GTSAC 2 cut(s) 1184, 2230
TspDTI ATGAA 3 cut(s) 17, 618, 645
TspRI CASTG 4 cut(s) 507, 530, 2196, 2251
VneI GTGCAC 3 cut(s) 73, 1608, 1797
VpaK11BI GGWCC 3 cut(s) 620, 1529, 1616
XapI RAATTY 1 cut(s) 2059
XceI RCATGY 4 cut(s) 22, 226, 1694, 1862
XhoI CTCGAG 2 cut(s) 1524, 2202
XmiI GTMKAC 3 cut(s) 1368, 1863, 2147
XmnI GAANNNNTTC 1 cut(s) 313
XspI CTAG 6 cut(s) 720, 771, 827, 1134, 1592, 1623
ZrmI AGTACT 1 cut(s) 1554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.