Prupe.1G425700_v2.0.a1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001634
Physical Location & Seq
Forward (+)
36635468 .. 36637780
2313 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G425700.1

Sequence Viewer

Length: 2313 bp
ATGAAGATACCCATTTGCATGCTTCCACTAACGCTTATGCTTCTGGCTCTCTCTCACATCTCGTGCGTGGTAGCAGAAGAGAGAGAGCAGCAGATGAATAAGACCTACATCATTCGCATGGACAAGTCCAAAATGCCTGCGAGTTTTGGCGACGACCATTTCCAATGGTATGGCTCATCTTTGAAATCGGTGTCCAAGTCAGCAGACGTGCTCTACACCTACAGAACCATAATTCACGGCTTCTCCACAAGGCTAACTGCTGAGGAAGCTGAGCTGCTTGAAAAACAAACAGGGGTTGTTTCTGTTTTACGTGAGTTAAGATATGAACTTCACACAACTCGGACACCCGAGTTCCTTGGAATAACCTTTGCCAGAAACGAGGCCATCTTCCCGACATCAGATAAAATAAGTAGGGTGGTTATTGGCTTAATTGACACCGGTGTCTGGCCTGAGATCAAGAGCTACGATGATAAAGGTCTTGGGCCAGTGCCAAAGACCTGGAAAGGGAAGTGCGAGGAAGGAACAAACTTCAACTCATCAAGTTGCAATAGAAAGCTAGTTGGTGCGAGATTTTTCCCAAAAGGATATGAAGCATCAATGGGACAACCCATTGATGAAAGAGTAGAATCAAGATCACCAAGAGATGATAGTGGCCATGGAACTCACACCTCGACCACTGCAGCCGGGTCTGCAGTCCCGGGAGCTAGCCTATTTGGTTATGCTTCGGGGACAGCAAGGGGTATGGCTACACAAGCTCGAGTAGCCACATACAAGGCCTGCTGGTTTGGTGGGTGTTACTCCTCTGATATTATAGCCGCAATGGATAAGGCTGTTGAAGATGGCGTCAACATTTTATCTTTGTCCATAGGGGGTACACGGTACCATAATTATTACACAGACGCTATGGCCATCGGAGCTTTCTCTGCCATGGCCAAGGGAATCTTTGTGTCAGGTTCAGCCGGAAATAGGGGGCCAGCTAAGGGGAGCTTGTCCCACAATGCACCTTGGATAACCACCGTGGGTGCTGGAACACTAGACCGTGATTTCCCGGCTTATGTTAGCCTTGGAAATAGAAAAAAATATAGAGGTATATCAATCTATGCCGGACCATCACTATCTTGTGGATTACTTCCACTTGTTTATGCTGGTAATGCAAGTAACTCCATAGATGGTGATCTATGCAGTCTTGACAGTCTGATTCCTGGAAAGGTTGCCGGGAAAATTGTGGTATGTGATAAAGGGATAACCTACAACTCTGAGAAGAGTGCGGTGGTAAAAAAGGCTGGTGGTCTAGGGATGATTTTGGCAAATACTAAAGCTTATGGAGAAGAGGTTGTTGCCGACTCGTATCTTTTGCCTACAGTAGTGGTTGGCCAGAAAGCTGGTGATGCAATAAAACGATATATTGCCTCCCATGATAATCCGAAAGCCACATTTGATTTTGGGAAAACGCAATTAGGAGTGGAGCCCTCGCCAGTGGTGGCAGCATTCAGCTCTAGAGGACCAAATCTAATCTCTCCAACCGTACTCAAACCGGACTTAATAGCACCAGGAGTGAATATCCTAGCTGGGTGGTCTGGTGCAGTTCCACCATCTGGATTTTATGAAGACACCAGGAGGGTGAGCTTCAACATCATTTCAGGTACATCCATGTCATGCCCACATGTGAGTGGGCTAGCTGCCCTTCTCAAGGCGGCTCACCCAAAATGGAGCCCTGCAGCCGTTAAGTCTGCTCTCATGACCACATCCTATACCACATACAAAAATGGAAAACCACTCAAAGATATTGCTACCAGAAATGCTGCAACACCATTCGATTACGGTGCCGGGCATGTGGACCCAGTGGCAGCCCTTGACCCAGGCCTTGTTTATGATCTTGGTGTCGAGGACTACCTAAGCTTCCTTTGTGCCTTGAATTATACAACAAGAGATATCAAGATACTCACCCACATAGACTTCACCTGTGATTCAAGCAAGAATTACAGGGCTGGAGATCTAAATTATCCATCTTTTGCTGTTTCTCTAAACACAAGTTCAGGCAACAGGGGAGCAGGCACTAAAATATACACAAGGACTCTCACAAATGTGGGTACCCCAGGAACATACAAGGTATCTGTTTCTGCACTATCACCATCAGTGAAGATCTGGGTTGAACCAAAATCACTGAGTTTCGCTCAAGCATATGAGAAGAAGATGTATACTGTAACCTTTGTGACCAGTGCTATGCCATCTGGTACAAAAAGCTTTGCTAACTTGGAATGGTCTGATGGGAAACATATAGTGAGTAGTCCTATTGCTATCAGCTGGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

771

Amino Acids

82.43

Weight (kDa)

8.98

Isoelectric Point (pI)

32.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 440
Acc65I GGTACC 2 cut(s) 879, 2090
AccB1I GGYRCC 3 cut(s) 879, 1823, 2090
AccB7I CCANNNNNTGG 1 cut(s) 1595
AccI GTMKAC 1 cut(s) 2198
AciI CCGC 3 cut(s) 816, 1268, 1694
AcoI YGGCCR 4 cut(s) 652, 906, 930, 1372
AcyI GRCGYC 1 cut(s) 843
AfaI GTAC 6 cut(s) 874, 881, 1527, 1645, 2092, 2236
AfiI CCNNNNNNNGG 6 cut(s) 444, 504, 966, 980, 1595, 1690
AflIII ACRYGT 1 cut(s) 1663
AgeI ACCGGT 1 cut(s) 437
AgsI TTSAA 8 cut(s) 184, 281, 532, 836, 1630, 1915, 1971, 2153
AjiI CACGTC 1 cut(s) 208
AjnI CCWGG 6 cut(s) 497, 1201, 1549, 1613, 1858, 2095
AleI CACNNNNGTG 1 cut(s) 2084
Alw21I GWGCWC 1 cut(s) 213
Ama87I CYCGRG 3 cut(s) 347, 697, 756
ApeKI GCWGC 8 cut(s) 88, 274, 680, 1484, 1679, 1718, 1802, 1847
AsiGI ACCGGT 1 cut(s) 437
Asp718I GGTACC 2 cut(s) 879, 2090
AspS9I GGNCC 5 cut(s) 482, 971, 1106, 1502, 1837
AsuC2I CCSGG 6 cut(s) 685, 698, 699, 1049, 1216, 1828
AsuHPI GGTGA 8 cut(s) 627, 1184, 1397, 1633, 1691, 1936, 1951, 2121
AsuNHI GCTAGC 2 cut(s) 704, 1675
AvaI CYCGRG 3 cut(s) 347, 697, 756
AvaII GGWCC 3 cut(s) 1106, 1502, 1837
BalI TGGCCA 4 cut(s) 654, 908, 932, 1374
BanI GGYRCC 3 cut(s) 879, 1823, 2090
BanII GRGCYC 2 cut(s) 1470, 1715
BauI CACGAG 1 cut(s) 61
BbsI GAAGAC 1 cut(s) 1614
Bbv12I GWGCWC 1 cut(s) 213
BbvCI CCTCAGC 1 cut(s) 261
BbvI GCAGC 8 cut(s) 100, 261, 692, 1496, 1666, 1730, 1789, 1859
BceAI ACGGC 2 cut(s) 253, 1706
BcgI CGANNNNNNTGC 2 cut(s) 1805, 1839
BciT130I CCWGG 6 cut(s) 499, 1203, 1551, 1615, 1860, 2097
BcnI CCSGG 6 cut(s) 685, 698, 699, 1049, 1216, 1828
BfaI CTAG 7 cut(s) 557, 705, 1034, 1292, 1497, 1565, 1676
BfmI CTRYAG 5 cut(s) 220, 678, 690, 1359, 1716
BglII AGATCT 2 cut(s) 1993, 2142
BlpI GCTNAGC 1 cut(s) 270
Bme18I GGWCC 3 cut(s) 1106, 1502, 1837
BmeT110I CYCGRG 3 cut(s) 347, 697, 756
BmgBI CACGTC 1 cut(s) 208
BmgT120I GGNCC 5 cut(s) 482, 971, 1106, 1502, 1837
BmiI GGNNCC 7 cut(s) 881, 972, 1467, 1712, 1825, 1839, 2092
BmrI ACTGGG 1 cut(s) 1835
BmsI GCATC 2 cut(s) 602, 1378
BmtI GCTAGC 2 cut(s) 708, 1679
BmuI ACTGGG 1 cut(s) 1835
BpiI GAAGAC 1 cut(s) 1614
BplI GAGNNNNNCTC 2 cut(s) 2158, 2190
BpmI CTGGAG 1 cut(s) 2010
Bpu10I CCTNAGC 3 cut(s) 261, 978, 1895
Bpu1102I GCTNAGC 1 cut(s) 270
BpuEI CTTGAG 2 cut(s) 1673, 2160
BpuMI CCSGG 6 cut(s) 685, 698, 699, 1049, 1216, 1828
BsaAI YACGTR 1 cut(s) 311
BsaBI GATNNNNATC 1 cut(s) 1173
BsaHI GRCGYC 1 cut(s) 843
BsaWI WCCGGW 2 cut(s) 437, 1534
BsaXI ACNNNNNCTCC 4 cut(s) 227, 257, 1317, 1347
Bsc4I CCNNNNNNNGG 6 cut(s) 444, 504, 966, 980, 1595, 1690
Bse118I RCCGGY 1 cut(s) 437
Bse1I ACTGG 4 cut(s) 485, 1475, 1841, 2217
Bse3DI GCAATG 1 cut(s) 825
Bse8I GATNNNNATC 1 cut(s) 1173
BseBI CCWGG 6 cut(s) 499, 1203, 1551, 1615, 1860, 2097
BseGI GGATG 3 cut(s) 1302, 1646, 1745
BseJI GATNNNNATC 1 cut(s) 1173
BseLI CCNNNNNNNGG 6 cut(s) 444, 504, 966, 980, 1595, 1690
BseMI GCAATG 1 cut(s) 825
BseMII CTCAG 5 cut(s) 252, 261, 441, 1248, 2156
BseNI ACTGG 4 cut(s) 485, 1475, 1841, 2217
BseRI GAGGAG 1 cut(s) 790
BseXI GCAGC 8 cut(s) 100, 261, 692, 1496, 1666, 1730, 1789, 1859
BseYI CCCAGC 1 cut(s) 1568
BsgI GTGCAG 2 cut(s) 1602, 2106
BshNI GGYRCC 3 cut(s) 879, 1823, 2090
BshTI ACCGGT 1 cut(s) 437
BsiHKAI GWGCWC 1 cut(s) 213
BsiHKCI CYCGRG 3 cut(s) 347, 697, 756
BsiSI CCGG 9 cut(s) 438, 684, 698, 960, 1049, 1104, 1215, 1535, 1827
BslFI GGGAC 4 cut(s) 615, 680, 742, 976
BslI CCNNNNNNNGG 6 cut(s) 444, 504, 966, 980, 1595, 1690
BsmFI GGGAC 4 cut(s) 615, 680, 742, 976
BsmI GAATGC 1 cut(s) 1487
BsoBI CYCGRG 3 cut(s) 347, 697, 756
Bsp1286I GDGCHC 3 cut(s) 213, 1470, 1715
Bsp143I GATC 6 cut(s) 453, 632, 1174, 1873, 1993, 2142
Bsp1720I GCTNAGC 1 cut(s) 270
Bsp19I CCATGG 2 cut(s) 655, 927
BspACI CCGC 3 cut(s) 816, 1268, 1694
BspCNI CTCAG 5 cut(s) 253, 262, 442, 1249, 2157
BspHI TCATGA 1 cut(s) 1737
BspLI GGNNCC 7 cut(s) 881, 972, 1467, 1712, 1825, 1839, 2092
BspMAI CTGCAG 3 cut(s) 682, 694, 1720
BspOI GCTAGC 2 cut(s) 708, 1679
BspT107I GGYRCC 3 cut(s) 879, 1823, 2090
BsrDI GCAATG 1 cut(s) 825
BsrFI RCCGGY 1 cut(s) 437
BsrI ACTGG 4 cut(s) 485, 1475, 1841, 2217
BssAI RCCGGY 1 cut(s) 437
BssMI GATC 6 cut(s) 453, 632, 1174, 1873, 1993, 2142
BssNAI GTATAC 1 cut(s) 2199
BssNI GRCGYC 1 cut(s) 843
BssSI CACGAG 1 cut(s) 61
BssT1I CCWWGG 6 cut(s) 355, 655, 927, 933, 1004, 1063
Bst1107I GTATAC 1 cut(s) 2199
Bst2BI CACGAG 1 cut(s) 61
Bst2UI CCWGG 6 cut(s) 499, 1203, 1551, 1615, 1860, 2097
Bst4CI ACNGT 8 cut(s) 879, 1018, 1040, 1193, 1363, 1525, 1823, 2203
Bst6I CTCTTC 3 cut(s) 72, 1256, 1323
BstACI GRCGYC 1 cut(s) 843
BstBAI YACGTR 1 cut(s) 311
BstC8I GCNNGC 7 cut(s) 20, 138, 706, 778, 975, 1677, 2053
BstDEI CTNAG 7 cut(s) 261, 270, 450, 978, 1257, 1895, 2165
BstDSI CCRYGG 3 cut(s) 655, 927, 1017
BstENI CCTNNNNNAGG 1 cut(s) 1688
BstF5I GGATG 3 cut(s) 1302, 1646, 1745
BstKTI GATC 6 cut(s) 456, 635, 1177, 1876, 1996, 2145
BstMBI GATC 6 cut(s) 453, 632, 1174, 1873, 1993, 2142
BstMWI GCNNNNNNNGC 8 cut(s) 266, 689, 752, 761, 914, 923, 1151, 1388
BstNI CCWGG 6 cut(s) 499, 1203, 1551, 1615, 1860, 2097
BstNSI RCATGY 3 cut(s) 22, 1667, 1835
BstSFI CTRYAG 5 cut(s) 220, 678, 690, 1359, 1716
BstV1I GCAGC 8 cut(s) 100, 261, 692, 1496, 1666, 1730, 1789, 1859
BstV2I GAAGAC 1 cut(s) 1614
BstX2I RGATCY 2 cut(s) 1993, 2142
BstXI CCANNNNNNTGG 3 cut(s) 170, 498, 1382
BstYI RGATCY 2 cut(s) 1993, 2142
BstZ17I GTATAC 1 cut(s) 2199
BtgI CCRYGG 3 cut(s) 655, 927, 1017
BtrI CACGTC 1 cut(s) 208
BtsCI GGATG 3 cut(s) 1302, 1646, 1745
BtsI GCAGTG 1 cut(s) 675
BtsIMutI CAGTG 7 cut(s) 492, 675, 1482, 1848, 2142, 2162, 2224
Cac8I GCNNGC 7 cut(s) 20, 138, 706, 778, 975, 1677, 2053
CciI TCATGA 1 cut(s) 1737
Cfr10I RCCGGY 1 cut(s) 437
Cfr13I GGNCC 5 cut(s) 482, 971, 1106, 1502, 1837
Cfr9I CCCGGG 1 cut(s) 697
CseI GACGC 2 cut(s) 832, 908
Csp6I GTAC 6 cut(s) 873, 880, 1526, 1644, 2091, 2235
CspAI ACCGGT 1 cut(s) 437
CviQI GTAC 6 cut(s) 873, 880, 1526, 1644, 2091, 2235
DdeI CTNAG 7 cut(s) 261, 270, 450, 978, 1257, 1895, 2165
DpnI GATC 6 cut(s) 455, 634, 1176, 1875, 1995, 2144
DpnII GATC 6 cut(s) 453, 632, 1174, 1873, 1993, 2142
DrdI GACNNNNNNGTC 1 cut(s) 440
DseDI GACNNNNNNGTC 1 cut(s) 440
EaeI YGGCCR 4 cut(s) 652, 906, 930, 1372
Eam1104I CTCTTC 3 cut(s) 72, 1256, 1323
EarI CTCTTC 3 cut(s) 72, 1256, 1323
Eco130I CCWWGG 6 cut(s) 355, 655, 927, 933, 1004, 1063
Eco147I AGGCCT 2 cut(s) 776, 1863
Eco24I GRGCYC 2 cut(s) 1470, 1715
Eco32I GATATC 1 cut(s) 1933
Eco47I GGWCC 3 cut(s) 1106, 1502, 1837
Eco88I CYCGRG 3 cut(s) 347, 697, 756
EcoNI CCTNNNNNAGG 1 cut(s) 1688
EcoRII CCWGG 6 cut(s) 497, 1201, 1549, 1613, 1858, 2095
EcoRV GATATC 1 cut(s) 1933
EcoT14I CCWWGG 6 cut(s) 355, 655, 927, 933, 1004, 1063
EcoT38I GRGCYC 2 cut(s) 1470, 1715
ErhI CCWWGG 6 cut(s) 355, 655, 927, 933, 1004, 1063
FalI AAGNNNNNCTT 4 cut(s) 926, 958, 971, 1003
FaqI GGGAC 4 cut(s) 615, 680, 742, 976
FauNDI CATATG 1 cut(s) 2182
FblI GTMKAC 1 cut(s) 2198
FokI GGATG 3 cut(s) 1309, 1633, 1732
FriOI GRGCYC 2 cut(s) 1470, 1715
FspBI CTAG 7 cut(s) 557, 705, 1034, 1292, 1497, 1565, 1676
GsaI CCCAGC 1 cut(s) 1572
GsuI CTGGAG 1 cut(s) 2010
HapII CCGG 9 cut(s) 438, 684, 698, 960, 1049, 1104, 1215, 1535, 1827
HgaI GACGC 2 cut(s) 832, 908
Hin1I GRCGYC 1 cut(s) 843
HincII GTYRAC 1 cut(s) 847
HindII GTYRAC 1 cut(s) 847
HindIII AAGCTT 3 cut(s) 1317, 1897, 2242
HinfI GANTC 6 cut(s) 626, 939, 1198, 1343, 1967, 2074
HpaII CCGG 9 cut(s) 438, 684, 698, 960, 1049, 1104, 1215, 1535, 1827
HphI GGTGA 8 cut(s) 627, 1184, 1397, 1633, 1691, 1936, 1951, 2121
Hpy166II GTNNAC 4 cut(s) 847, 875, 1837, 2199
Hpy188I TCNGA 8 cut(s) 342, 400, 805, 914, 1197, 1258, 1425, 2266
Hpy188III TCNNGA 8 cut(s) 391, 457, 630, 1187, 1497, 1596, 1738, 1936
Hpy8I GTNNAC 4 cut(s) 847, 875, 1837, 2199
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 2 cut(s) 512, 1694
HpyCH4III ACNGT 8 cut(s) 879, 1018, 1040, 1193, 1363, 1525, 1823, 2203
HpyCH4IV ACGT 2 cut(s) 207, 310
HpyF10VI GCNNNNNNNGC 8 cut(s) 266, 689, 752, 761, 914, 923, 1151, 1388
HpyF3I CTNAG 7 cut(s) 261, 270, 450, 978, 1257, 1895, 2165
HpySE526I ACGT 2 cut(s) 207, 310
Hsp92I GRCGYC 1 cut(s) 843
KpnI GGTACC 2 cut(s) 883, 2094
Kzo9I GATC 6 cut(s) 453, 632, 1174, 1873, 1993, 2142
LmnI GCTCC 6 cut(s) 701, 914, 984, 1465, 1710, 2048
Lsp1109I GCAGC 8 cut(s) 100, 261, 692, 1496, 1666, 1730, 1789, 1859
LweI GCATC 2 cut(s) 602, 1378
MaeI CTAG 7 cut(s) 557, 705, 1034, 1292, 1497, 1565, 1676
MaeII ACGT 2 cut(s) 207, 310
MaeIII GTNAC 4 cut(s) 794, 1157, 2203, 2212
MalI GATC 6 cut(s) 455, 634, 1176, 1875, 1995, 2144
MboI GATC 6 cut(s) 453, 632, 1174, 1873, 1993, 2142
MflI RGATCY 2 cut(s) 1993, 2142
MhlI GDGCHC 3 cut(s) 213, 1470, 1715
MlsI TGGCCA 4 cut(s) 654, 908, 932, 1374
MluCI AATT 8 cut(s) 231, 429, 886, 1221, 1454, 1915, 1978, 1999
MluNI TGGCCA 4 cut(s) 654, 908, 932, 1374
MlyI GAGTC 2 cut(s) 1337, 2068
MmeI TCCRAC 1 cut(s) 1544
Mox20I TGGCCA 4 cut(s) 654, 908, 932, 1374
MscI TGGCCA 4 cut(s) 654, 908, 932, 1374
MseI TTAA 5 cut(s) 317, 428, 1541, 1725, 2311
MslI CAYNNNNRTG 4 cut(s) 17, 116, 1668, 2084
Msp20I TGGCCA 4 cut(s) 654, 908, 932, 1374
MspA1I CMGCKG 1 cut(s) 2304
MspI CCGG 9 cut(s) 438, 684, 698, 960, 1049, 1104, 1215, 1535, 1827
Mva1269I GAATGC 1 cut(s) 1487
MvaI CCWGG 6 cut(s) 499, 1203, 1551, 1615, 1860, 2097
MwoI GCNNNNNNNGC 8 cut(s) 266, 689, 752, 761, 914, 923, 1151, 1388
NciI CCSGG 6 cut(s) 685, 698, 699, 1049, 1216, 1828
NcoI CCATGG 2 cut(s) 655, 927
NdeI CATATG 1 cut(s) 2182
NdeII GATC 6 cut(s) 453, 632, 1174, 1873, 1993, 2142
NheI GCTAGC 2 cut(s) 704, 1675
NlaIV GGNNCC 7 cut(s) 881, 972, 1467, 1712, 1825, 1839, 2092
NmuCI GTSAC 1 cut(s) 2212
NspI RCATGY 3 cut(s) 22, 1667, 1835
OliI CACNNNNGTG 1 cut(s) 2084
PaeI GCATGC 1 cut(s) 22
PaeR7I CTCGAG 1 cut(s) 756
PagI TCATGA 1 cut(s) 1737
PceI AGGCCT 2 cut(s) 776, 1863
PciI ACATGT 1 cut(s) 1663
PctI GAATGC 1 cut(s) 1487
PfeI GAWTC 4 cut(s) 626, 939, 1198, 1967
PflMI CCANNNNNTGG 1 cut(s) 1595
PfoI TCCNGGA 1 cut(s) 1201
PinAI ACCGGT 1 cut(s) 437
PleI GAGTC 2 cut(s) 1337, 2068
PpsI GAGTC 2 cut(s) 1337, 2068
Ppu21I YACGTR 1 cut(s) 311
PscI ACATGT 1 cut(s) 1663
Psp6I CCWGG 6 cut(s) 497, 1201, 1549, 1613, 1858, 2095
PspFI CCCAGC 1 cut(s) 1568
PspGI CCWGG 6 cut(s) 497, 1201, 1549, 1613, 1858, 2095
PspN4I GGNNCC 7 cut(s) 881, 972, 1467, 1712, 1825, 1839, 2092
PspPI GGNCC 5 cut(s) 482, 971, 1106, 1502, 1837
PspXI VCTCGAGB 1 cut(s) 756
PstI CTGCAG 3 cut(s) 682, 694, 1720
PsuI RGATCY 2 cut(s) 1993, 2142
PvuII CAGCTG 1 cut(s) 2304
RsaI GTAC 6 cut(s) 874, 881, 1527, 1645, 2092, 2236
RsaNI GTAC 6 cut(s) 873, 880, 1526, 1644, 2091, 2235
RseI CAYNNNNRTG 4 cut(s) 17, 116, 1668, 2084
SaqAI TTAA 5 cut(s) 317, 428, 1541, 1725, 2311
Sau3AI GATC 6 cut(s) 453, 632, 1174, 1873, 1993, 2142
Sau96I GGNCC 5 cut(s) 482, 971, 1106, 1502, 1837
SchI GAGTC 2 cut(s) 1337, 2068
SduI GDGCHC 3 cut(s) 213, 1470, 1715
SfaNI GCATC 2 cut(s) 602, 1378
SfcI CTRYAG 5 cut(s) 220, 678, 690, 1359, 1716
Sfr274I CTCGAG 1 cut(s) 756
SgrAI CRCCGGYG 1 cut(s) 437
SinI GGWCC 3 cut(s) 1106, 1502, 1837
SlaI CTCGAG 1 cut(s) 756
SmaI CCCGGG 1 cut(s) 699
SmiMI CAYNNNNRTG 4 cut(s) 17, 116, 1668, 2084
SmlI CTYRAG 3 cut(s) 756, 1688, 2175
SmoI CTYRAG 3 cut(s) 756, 1688, 2175
SphI GCATGC 1 cut(s) 22
Sse9I AATT 8 cut(s) 231, 429, 886, 1221, 1454, 1915, 1978, 1999
SseBI AGGCCT 2 cut(s) 776, 1863
SsiI CCGC 3 cut(s) 816, 1268, 1694
SspMI CTAG 7 cut(s) 557, 705, 1034, 1292, 1497, 1565, 1676
StuI AGGCCT 2 cut(s) 776, 1863
StyI CCWWGG 6 cut(s) 355, 655, 927, 933, 1004, 1063
TaaI ACNGT 8 cut(s) 879, 1018, 1040, 1193, 1363, 1525, 1823, 2203
TaiI ACGT 2 cut(s) 210, 313
TaqI TCGA 4 cut(s) 671, 757, 1815, 1884
TasI AATT 8 cut(s) 231, 429, 886, 1221, 1454, 1915, 1978, 1999
TauI GCSGC 2 cut(s) 818, 1697
TfiI GAWTC 4 cut(s) 626, 939, 1198, 1967
Tru1I TTAA 5 cut(s) 317, 428, 1541, 1725, 2311
Tru9I TTAA 5 cut(s) 317, 428, 1541, 1725, 2311
TscAI CASTG 7 cut(s) 492, 682, 1482, 1848, 2142, 2169, 2224
TseFI GTSAC 1 cut(s) 2212
TseI GCWGC 8 cut(s) 88, 274, 680, 1484, 1679, 1718, 1802, 1847
Tsp45I GTSAC 1 cut(s) 2212
TspDTI ATGAA 6 cut(s) 17, 110, 339, 603, 630, 1620
TspMI CCCGGG 1 cut(s) 697
TspRI CASTG 7 cut(s) 492, 682, 1482, 1848, 2142, 2169, 2224
Van91I CCANNNNNTGG 1 cut(s) 1595
VpaK11BI GGWCC 3 cut(s) 1106, 1502, 1837
XagI CCTNNNNNAGG 1 cut(s) 1688
XbaI TCTAGA 1 cut(s) 1496
XceI RCATGY 3 cut(s) 22, 1667, 1835
XhoI CTCGAG 1 cut(s) 756
XmaI CCCGGG 1 cut(s) 697
XmiI GTMKAC 1 cut(s) 2198
XspI CTAG 7 cut(s) 557, 705, 1034, 1292, 1497, 1565, 1676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.