Rorug06G0370600

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001634
Physical Location & Seq
Reverse (-)
51772383 .. 51773148
766 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0370600.1

Sequence Viewer

Length: 363 bp
ATGTGCCTCCTGCGTGACTGCAACGTCTACGAAAGCCCAAAAAATGAATTTTCAGAACCTTTCAAGAGGCCCTTTCACCGTGGCCTTCCATCCGACAGTTTGGTTTGTCAAGATGACAAGCTGCAGGGGTGTGTTGACCGCACTCTAGACAGTAGGGCTTTCAGAGGCTGGATTGAGACAGATAACCCTTGGACAAATGATGACGAGACTGATAATGATGAGATAACATATGTGAAACTTCTACTGAACCCTGAACGTTACACTGGCTATGCTGGTCCATCAGCAAGAAGGATATGGGATGCTGTTTATTCTGAAAATTGCCCAAATTGTGAGTCGAAACTTTTGGTAGATCTACTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

120

Amino Acids

13.85

Weight (kDa)

4.56

Isoelectric Point (pI)

43.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ERO1 PF04137 1 - 109 1.7e-19 Endoplasmic Reticulum Oxidoreductin 1 (ERO1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 23
AccI GTMKAC 1 cut(s) 27
AciI CCGC 1 cut(s) 139
AclI AACGTT 1 cut(s) 256
AcsI RAATTY 1 cut(s) 47
AgsI TTSAA 1 cut(s) 64
AluBI AGCT 1 cut(s) 121
AluI AGCT 1 cut(s) 121
Alw26I GTCTC 2 cut(s) 170, 200
AlwNI CAGNNNCTG 1 cut(s) 168
AoxI GGCC 2 cut(s) 68, 82
ApeKI GCWGC 1 cut(s) 121
ApoI RAATTY 1 cut(s) 47
ArsI GACNNNNNNTTYG 2 cut(s) 317, 349
AspS9I GGNCC 2 cut(s) 69, 275
AsuHPI GGTGA 1 cut(s) 68
AvaII GGWCC 1 cut(s) 275
BbvI GCAGC 1 cut(s) 108
BccI CCATC 2 cut(s) 97, 286
BcoDI GTCTC 2 cut(s) 170, 200
BfaI CTAG 1 cut(s) 146
BfmI CTRYAG 1 cut(s) 122
BglII AGATCT 1 cut(s) 349
BisI GCNGC 1 cut(s) 122
BlsI GCNGC 1 cut(s) 123
Bme18I GGWCC 1 cut(s) 275
BmgT120I GGNCC 2 cut(s) 69, 275
BmsI GCATC 1 cut(s) 289
BsaJI CCNNGG 2 cut(s) 79, 188
Bse1I ACTGG 1 cut(s) 268
BseDI CCNNGG 2 cut(s) 79, 188
BseGI GGATG 2 cut(s) 89, 304
BseNI ACTGG 1 cut(s) 268
BseXI GCAGC 1 cut(s) 108
BshFI GGCC 2 cut(s) 70, 84
BsmAI GTCTC 2 cut(s) 170, 200
BsnI GGCC 2 cut(s) 70, 84
Bsp143I GATC 1 cut(s) 349
BspACI CCGC 1 cut(s) 139
BspANI GGCC 2 cut(s) 70, 84
BspMAI CTGCAG 1 cut(s) 126
BsrI ACTGG 1 cut(s) 268
BssECI CCNNGG 2 cut(s) 79, 188
BssMI GATC 1 cut(s) 349
BssT1I CCWWGG 1 cut(s) 188
Bst4CI ACNGT 3 cut(s) 80, 98, 152
BstDSI CCRYGG 1 cut(s) 79
BstF5I GGATG 2 cut(s) 89, 304
BstKTI GATC 1 cut(s) 352
BstMAI GTCTC 2 cut(s) 170, 200
BstMBI GATC 1 cut(s) 349
BstSFI CTRYAG 1 cut(s) 122
BstV1I GCAGC 1 cut(s) 108
BstX2I RGATCY 1 cut(s) 349
BstYI RGATCY 1 cut(s) 349
BsuRI GGCC 2 cut(s) 70, 84
BtgI CCRYGG 1 cut(s) 79
BtsCI GGATG 2 cut(s) 89, 304
BtsIMutI CAGTG 1 cut(s) 261
CaiI CAGNNNCTG 1 cut(s) 168
Cfr13I GGNCC 2 cut(s) 69, 275
CviJI RGCY 7 cut(s) 36, 70, 84, 121, 158, 168, 267
CviKI_1 RGCY 7 cut(s) 36, 70, 84, 121, 158, 168, 267
DpnI GATC 1 cut(s) 351
DpnII GATC 1 cut(s) 349
DrdI GACNNNNNNGTC 1 cut(s) 23
DseDI GACNNNNNNGTC 1 cut(s) 23
Eco130I CCWWGG 1 cut(s) 188
Eco47I GGWCC 1 cut(s) 275
EcoO109I RGGNCCY 1 cut(s) 69
EcoT14I CCWWGG 1 cut(s) 188
ErhI CCWWGG 1 cut(s) 188
FaiI YATR 4 cut(s) 229, 231, 270, 295
FalI AAGNNNNNCTT 2 cut(s) 56, 88
FauNDI CATATG 1 cut(s) 229
FblI GTMKAC 1 cut(s) 27
Fnu4HI GCNGC 1 cut(s) 122
FokI GGATG 2 cut(s) 76, 311
Fsp4HI GCNGC 1 cut(s) 122
FspBI CTAG 1 cut(s) 146
GluI GCNGC 1 cut(s) 122
HaeIII GGCC 2 cut(s) 70, 84
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HinfI GANTC 1 cut(s) 332
HphI GGTGA 1 cut(s) 68
Hpy166II GTNNAC 2 cut(s) 28, 136
Hpy188I TCNGA 4 cut(s) 55, 94, 164, 313
Hpy188III TCNNGA 3 cut(s) 64, 110, 146
Hpy8I GTNNAC 2 cut(s) 28, 136
HpyAV CCTTC 2 cut(s) 95, 282
HpyCH4III ACNGT 3 cut(s) 80, 98, 152
HpyCH4IV ACGT 2 cut(s) 24, 256
HpyCH4V TGCA 2 cut(s) 21, 124
HpySE526I ACGT 2 cut(s) 24, 256
Kzo9I GATC 1 cut(s) 349
LpnPI CCDG 6 cut(s) 23, 110, 154, 249, 258, 264
Lsp1109I GCAGC 1 cut(s) 108
LweI GCATC 1 cut(s) 289
MaeI CTAG 1 cut(s) 146
MaeII ACGT 2 cut(s) 24, 256
MaeIII GTNAC 2 cut(s) 14, 257
MalI GATC 1 cut(s) 351
MboI GATC 1 cut(s) 349
MflI RGATCY 1 cut(s) 349
MluCI AATT 3 cut(s) 47, 316, 325
MlyI GAGTC 1 cut(s) 341
MmeI TCCRAC 1 cut(s) 117
MnlI CCTC 3 cut(s) 17, 60, 158
NdeI CATATG 1 cut(s) 229
NdeII GATC 1 cut(s) 349
NmuCI GTSAC 1 cut(s) 14
PkrI GCNGC 1 cut(s) 123
PleI GAGTC 1 cut(s) 340
PpsI GAGTC 1 cut(s) 340
Psp1406I AACGTT 1 cut(s) 256
PspPI GGNCC 2 cut(s) 69, 275
PstI CTGCAG 1 cut(s) 126
PstNI CAGNNNCTG 1 cut(s) 168
PsuI RGATCY 1 cut(s) 349
SatI GCNGC 1 cut(s) 122
Sau3AI GATC 1 cut(s) 349
Sau96I GGNCC 2 cut(s) 69, 275
SchI GAGTC 1 cut(s) 341
SetI ASST 4 cut(s) 27, 61, 123, 259
SfaNI GCATC 1 cut(s) 289
SfcI CTRYAG 1 cut(s) 122
SinI GGWCC 1 cut(s) 275
Sse9I AATT 3 cut(s) 47, 316, 325
SsiI CCGC 1 cut(s) 139
SspMI CTAG 1 cut(s) 146
StyI CCWWGG 1 cut(s) 188
TaaI ACNGT 3 cut(s) 80, 98, 152
TaiI ACGT 2 cut(s) 27, 259
TaqI TCGA 1 cut(s) 335
TasI AATT 3 cut(s) 47, 316, 325
TscAI CASTG 1 cut(s) 268
TseFI GTSAC 1 cut(s) 14
TseI GCWGC 1 cut(s) 121
Tsp45I GTSAC 1 cut(s) 14
TspDTI ATGAA 1 cut(s) 60
TspRI CASTG 1 cut(s) 268
VpaK11BI GGWCC 1 cut(s) 275
XapI RAATTY 1 cut(s) 47
XbaI TCTAGA 1 cut(s) 145
XmiI GTMKAC 1 cut(s) 27
XspI CTAG 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.