Rmu_co8090004.1_g000001

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8090004.1
OG0001634
Physical Location & Seq
Forward (+)
1 .. 592
592 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8090004.1_g000001.1.cds

Sequence Viewer

Length: 592 bp
agtaaggtggtcatcggacttgtagacactggtgtatggcctgagagcaaaagctgcaatgacaagggtcttgcacctgtgccaaggagctggaaagggcagtgctgcgaggaggttcaggacttcaactcctcaagctgcaacaggaaactgattggcgccaggtttttctcaaaagggtatgaagcagcacttggacccatcaatgaaaagttagaatcaagatcaccacgagatgatgatggccatggaactcacacctccagcattgcagctggatcccctgttccaggagctagcctctttggttatgcgtcagggacagcacggggaatggctgcacaagctcgtgttgccatatataaggcatgctggcttggaggttgtttcggctctgatgttccagctgcaatggacaaggctattaaagatggtgtcaacattttatccttgtctttgtcttttccgggagcacaatacgaggatttctccacagacagtcagacattgttgctattggagctttttctgccatggccaagggagtatttgtgtcatgttcagccggaaatgatgggccaatttcctatac
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

197

Amino Acids

21.15

Weight (kDa)

5.61

Isoelectric Point (pI)

43.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 158
AccI GTMKAC 1 cut(s) 24
AclWI GGATC 2 cut(s) 273, 286
AcoI YGGCCR 2 cut(s) 244, 536
AcyI GRCGYC 1 cut(s) 159
AfiI CCNNNNNNNGG 1 cut(s) 290
AgsI TTSAA 1 cut(s) 127
AjnI CCWGG 2 cut(s) 161, 289
AjuI GAANNNNNNNTTGG 2 cut(s) 177, 209
AloI GAACNNNNNNTCC 2 cut(s) 270, 302
AluBI AGCT 8 cut(s) 54, 90, 138, 275, 296, 347, 407, 523
AluI AGCT 8 cut(s) 54, 90, 138, 275, 296, 347, 407, 523
Alw21I GWGCWC 1 cut(s) 475
AlwI GGATC 2 cut(s) 273, 286
AoxI GGCC 4 cut(s) 38, 244, 536, 577
ApeKI GCWGC 7 cut(s) 54, 105, 138, 188, 272, 338, 407
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 2 cut(s) 197, 577
AsuC2I CCSGG 1 cut(s) 468
AsuHPI GGTGA 1 cut(s) 219
AsuNHI GCTAGC 1 cut(s) 296
AvaII GGWCC 1 cut(s) 197
BalI TGGCCA 2 cut(s) 246, 538
BamHI GGATCC 1 cut(s) 278
BanI GGYRCC 1 cut(s) 158
BauI CACGAG 2 cut(s) 231, 348
Bbv12I GWGCWC 1 cut(s) 475
BbvI GCAGC 7 cut(s) 41, 92, 125, 200, 284, 325, 394
BccI CCATC 4 cut(s) 209, 236, 425, 568
BciT130I CCWGG 2 cut(s) 163, 291
BcnI CCSGG 1 cut(s) 468
BfaI CTAG 1 cut(s) 297
BfoI RGCGCY 1 cut(s) 162
BisI GCNGC 7 cut(s) 55, 106, 139, 189, 273, 339, 408
BlsI GCNGC 7 cut(s) 56, 107, 140, 190, 274, 340, 409
Bme1390I CCNGG 3 cut(s) 163, 291, 468
Bme18I GGWCC 1 cut(s) 197
BmgT120I GGNCC 2 cut(s) 197, 577
BmiI GGNNCC 3 cut(s) 160, 199, 280
BmrFI CCNGG 3 cut(s) 163, 291, 468
BmtI GCTAGC 1 cut(s) 300
BoxI GACNNNNGTC 1 cut(s) 66
BplI GAGNNNNNCTC 4 cut(s) 285, 317, 473, 505
BpmI CTGGAG 1 cut(s) 247
BpuEI CTTGAG 1 cut(s) 118
BpuMI CCSGG 1 cut(s) 468
BsaHI GRCGYC 1 cut(s) 159
BsaJI CCNNGG 4 cut(s) 83, 247, 533, 539
BsaXI ACNNNNNCTCC 4 cut(s) 113, 143, 536, 566
Bsc4I CCNNNNNNNGG 1 cut(s) 290
Bse1I ACTGG 1 cut(s) 34
Bse3DI GCAATG 3 cut(s) 64, 267, 417
BseBI CCWGG 2 cut(s) 163, 291
BseDI CCNNGG 4 cut(s) 83, 247, 533, 539
BseLI CCNNNNNNNGG 1 cut(s) 290
BseMI GCAATG 3 cut(s) 64, 267, 417
BseMII CTCAG 1 cut(s) 33
BseNI ACTGG 1 cut(s) 34
BseRI GAGGAG 2 cut(s) 121, 125
BseXI GCAGC 7 cut(s) 41, 92, 125, 200, 284, 325, 394
BsgI GTGCAG 1 cut(s) 324
BshFI GGCC 4 cut(s) 40, 246, 538, 579
BshNI GGYRCC 1 cut(s) 158
BsiHKAI GWGCWC 1 cut(s) 475
BsiSI CCGG 2 cut(s) 467, 566
BslFI GGGAC 1 cut(s) 334
BslI CCNNNNNNNGG 1 cut(s) 290
BsmFI GGGAC 1 cut(s) 334
BsnI GGCC 4 cut(s) 40, 246, 538, 579
Bsp1286I GDGCHC 1 cut(s) 475
Bsp143I GATC 2 cut(s) 224, 278
Bsp19I CCATGG 2 cut(s) 247, 533
BspANI GGCC 4 cut(s) 40, 246, 538, 579
BspCNI CTCAG 1 cut(s) 34
BspLI GGNNCC 3 cut(s) 160, 199, 280
BspOI GCTAGC 1 cut(s) 300
BspPI GGATC 2 cut(s) 273, 286
BspT107I GGYRCC 1 cut(s) 158
BsrDI GCAATG 3 cut(s) 64, 267, 417
BsrI ACTGG 1 cut(s) 34
BssECI CCNNGG 4 cut(s) 83, 247, 533, 539
BssMI GATC 2 cut(s) 224, 278
BssNI GRCGYC 1 cut(s) 159
BssSI CACGAG 2 cut(s) 231, 348
BssT1I CCWWGG 4 cut(s) 83, 247, 533, 539
Bst2BI CACGAG 2 cut(s) 231, 348
Bst2UI CCWGG 2 cut(s) 163, 291
Bst4CI ACNGT 1 cut(s) 500
BstACI GRCGYC 1 cut(s) 159
BstAPI GCANNNNNTGC 1 cut(s) 54
BstC8I GCNNGC 3 cut(s) 298, 370, 374
BstDEI CTNAG 1 cut(s) 42
BstDSI CCRYGG 2 cut(s) 247, 533
BstENI CCTNNNNNAGG 1 cut(s) 288
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 2 cut(s) 227, 281
BstMBI GATC 2 cut(s) 224, 278
BstMWI GCNNNNNNNGC 5 cut(s) 54, 344, 353, 520, 529
BstNI CCWGG 2 cut(s) 163, 291
BstNSI RCATGY 1 cut(s) 372
BstPAI GACNNNNGTC 1 cut(s) 66
BstSCI CCNGG 3 cut(s) 161, 289, 466
BstV1I GCAGC 7 cut(s) 41, 92, 125, 200, 284, 325, 394
BstX2I RGATCY 1 cut(s) 278
BstXI CCANNNNNNTGG 1 cut(s) 90
BstYI RGATCY 1 cut(s) 278
BsuRI GGCC 4 cut(s) 40, 246, 538, 579
BtgI CCRYGG 2 cut(s) 247, 533
BtsI GCAGTG 1 cut(s) 107
BtsIMutI CAGTG 2 cut(s) 27, 107
Cac8I GCNNGC 3 cut(s) 298, 370, 374
CfoI GCGC 1 cut(s) 161
Cfr13I GGNCC 2 cut(s) 197, 577
CseI GACGC 1 cut(s) 303
CviAII CATG 4 cut(s) 248, 369, 534, 557
DdeI CTNAG 1 cut(s) 42
DinI GGCGCC 1 cut(s) 160
DpnI GATC 2 cut(s) 226, 280
DpnII GATC 2 cut(s) 224, 278
EaeI YGGCCR 2 cut(s) 244, 536
Eco130I CCWWGG 4 cut(s) 83, 247, 533, 539
Eco47I GGWCC 1 cut(s) 197
EcoNI CCTNNNNNAGG 1 cut(s) 288
EcoRII CCWGG 2 cut(s) 161, 289
EcoT14I CCWWGG 4 cut(s) 83, 247, 533, 539
EgeI GGCGCC 1 cut(s) 160
EheI GGCGCC 1 cut(s) 160
ErhI CCWWGG 4 cut(s) 83, 247, 533, 539
FaeI CATG 4 cut(s) 251, 372, 537, 560
FalI AAGNNNNNCTT 2 cut(s) 177, 209
FaqI GGGAC 1 cut(s) 334
FatI CATG 4 cut(s) 247, 368, 533, 556
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 7 cut(s) 55, 106, 139, 189, 273, 339, 408
Fsp4HI GCNGC 7 cut(s) 55, 106, 139, 189, 273, 339, 408
FspBI CTAG 1 cut(s) 297
GlaI GCGC 1 cut(s) 160
GluI GCNGC 7 cut(s) 55, 106, 139, 189, 273, 339, 408
GsuI CTGGAG 1 cut(s) 247
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 4 cut(s) 40, 246, 538, 579
HapII CCGG 2 cut(s) 467, 566
HgaI GACGC 1 cut(s) 303
HhaI GCGC 1 cut(s) 161
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 4 cut(s) 251, 372, 537, 560
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HinfI GANTC 1 cut(s) 218
HpaII CCGG 2 cut(s) 467, 566
HphI GGTGA 1 cut(s) 219
Hpy166II GTNNAC 2 cut(s) 25, 439
Hpy188I TCNGA 3 cut(s) 17, 397, 504
Hpy188III TCNNGA 2 cut(s) 119, 222
Hpy8I GTNNAC 2 cut(s) 25, 439
HpyCH4III ACNGT 1 cut(s) 500
HpyCH4V TGCA 6 cut(s) 57, 74, 141, 272, 341, 410
HpyF10VI GCNNNNNNNGC 5 cut(s) 54, 344, 353, 520, 529
HpyF3I CTNAG 1 cut(s) 42
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 4 cut(s) 251, 372, 537, 560
HspAI GCGC 1 cut(s) 159
KasI GGCGCC 1 cut(s) 158
Kzo9I GATC 2 cut(s) 224, 278
LmnI GCTCC 4 cut(s) 87, 293, 470, 520
Lsp1109I GCAGC 7 cut(s) 41, 92, 125, 200, 284, 325, 394
MaeI CTAG 1 cut(s) 297
MalI GATC 2 cut(s) 226, 280
MboI GATC 2 cut(s) 224, 278
MflI RGATCY 1 cut(s) 278
MhlI GDGCHC 1 cut(s) 475
MlsI TGGCCA 2 cut(s) 246, 538
MluCI AATT 1 cut(s) 581
MluNI TGGCCA 2 cut(s) 246, 538
Mly113I GGCGCC 1 cut(s) 159
MnlI CCTC 7 cut(s) 103, 106, 142, 271, 311, 374, 475
Mox20I TGGCCA 2 cut(s) 246, 538
MscI TGGCCA 2 cut(s) 246, 538
MseI TTAA 1 cut(s) 426
Msp20I TGGCCA 2 cut(s) 246, 538
MspA1I CMGCKG 2 cut(s) 275, 407
MspI CCGG 2 cut(s) 467, 566
MspR9I CCNGG 3 cut(s) 163, 291, 468
MvaI CCWGG 2 cut(s) 163, 291
MwoI GCNNNNNNNGC 5 cut(s) 54, 344, 353, 520, 529
NarI GGCGCC 1 cut(s) 159
NciI CCSGG 1 cut(s) 468
NcoI CCATGG 2 cut(s) 247, 533
NdeII GATC 2 cut(s) 224, 278
NheI GCTAGC 1 cut(s) 296
NlaIII CATG 4 cut(s) 251, 372, 537, 560
NlaIV GGNNCC 3 cut(s) 160, 199, 280
NspI RCATGY 1 cut(s) 372
PaeI GCATGC 1 cut(s) 372
PfeI GAWTC 1 cut(s) 218
PfoI TCCNGGA 2 cut(s) 289, 466
PkrI GCNGC 7 cut(s) 56, 107, 140, 190, 274, 340, 409
PluTI GGCGCC 1 cut(s) 162
PshAI GACNNNNGTC 1 cut(s) 66
Psp6I CCWGG 2 cut(s) 161, 289
PspGI CCWGG 2 cut(s) 161, 289
PspN4I GGNNCC 3 cut(s) 160, 199, 280
PspPI GGNCC 2 cut(s) 197, 577
PsuI RGATCY 1 cut(s) 278
PvuII CAGCTG 2 cut(s) 275, 407
SaqAI TTAA 1 cut(s) 426
SatI GCNGC 7 cut(s) 55, 106, 139, 189, 273, 339, 408
Sau3AI GATC 2 cut(s) 224, 278
Sau96I GGNCC 2 cut(s) 197, 577
ScrFI CCNGG 3 cut(s) 163, 291, 468
SduI GDGCHC 1 cut(s) 475
SfoI GGCGCC 1 cut(s) 160
SinI GGWCC 1 cut(s) 197
SmlI CTYRAG 1 cut(s) 133
SmoI CTYRAG 1 cut(s) 133
SphI GCATGC 1 cut(s) 372
Sse9I AATT 1 cut(s) 581
SspDI GGCGCC 1 cut(s) 158
SspMI CTAG 1 cut(s) 297
StyD4I CCNGG 3 cut(s) 161, 289, 466
StyI CCWWGG 4 cut(s) 83, 247, 533, 539
TaaI ACNGT 1 cut(s) 500
TasI AATT 1 cut(s) 581
TfiI GAWTC 1 cut(s) 218
Tru1I TTAA 1 cut(s) 426
Tru9I TTAA 1 cut(s) 426
TscAI CASTG 2 cut(s) 34, 107
TseI GCWGC 7 cut(s) 54, 105, 138, 188, 272, 338, 407
TspDTI ATGAA 2 cut(s) 198, 222
TspRI CASTG 2 cut(s) 34, 107
VpaK11BI GGWCC 1 cut(s) 197
XagI CCTNNNNNAGG 1 cut(s) 288
XceI RCATGY 1 cut(s) 372
XmiI GTMKAC 1 cut(s) 24
XspI CTAG 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.