subtilisin-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 4 cut(s) | 398, 419, 818, 1220 |
| AatII | GACGTC | 1 cut(s) | 396 |
| AccB1I | GGYRCC | 3 cut(s) | 542, 1427, 1559 |
| AccBSI | CCGCTC | 2 cut(s) | 1650, 2010 |
| AccI | GTMKAC | 2 cut(s) | 1587, 1848 |
| AclI | AACGTT | 1 cut(s) | 825 |
| AcsI | RAATTY | 1 cut(s) | 1999 |
| AcvI | CACGTG | 3 cut(s) | 489, 1645, 1813 |
| AcyI | GRCGYC | 5 cut(s) | 242, 393, 450, 1109, 1560 |
| AfaI | GTAC | 6 cut(s) | 155, 308, 551, 956, 2042, 2081 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 277, 376, 660, 714, 1423, 1600, 1825, 1837 |
| AflIII | ACRYGT | 3 cut(s) | 159, 488, 1810 |
| AgeI | ACCGGT | 1 cut(s) | 1574 |
| AgsI | TTSAA | 5 cut(s) | 304, 511, 1609, 1894, 2038 |
| AjiI | CACGTC | 2 cut(s) | 1626, 1765 |
| AjnI | CCWGG | 4 cut(s) | 545, 605, 731, 1528 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 264, 296 |
| AleI | CACNNNNGTG | 1 cut(s) | 2060 |
| Alw21I | GWGCWC | 4 cut(s) | 92, 141, 315, 929 |
| Alw26I | GTCTC | 2 cut(s) | 251, 612 |
| Ama87I | CYCGRG | 7 cut(s) | 275, 296, 332, 1230, 1476, 2044, 2228 |
| ApaI | GGGCCC | 1 cut(s) | 2069 |
| ApeKI | GCWGC | 4 cut(s) | 235, 1333, 1663, 2214 |
| ApoI | RAATTY | 1 cut(s) | 1999 |
| AsiGI | ACCGGT | 1 cut(s) | 1574 |
| AspLEI | GCGC | 9 cut(s) | 700, 712, 953, 998, 1528, 1562, 1599, 1663, 1714 |
| AsuHPI | GGTGA | 7 cut(s) | 664, 1201, 1264, 2017, 2047, 2125, 2191 |
| AsuNHI | GCTAGC | 1 cut(s) | 680 |
| AvaI | CYCGRG | 7 cut(s) | 275, 296, 332, 1230, 1476, 2044, 2228 |
| BaeGI | GKGCMC | 4 cut(s) | 488, 1004, 1430, 2069 |
| BalI | TGGCCA | 4 cut(s) | 5, 427, 722, 794 |
| BamHI | GGATCC | 2 cut(s) | 704, 1393 |
| BanI | GGYRCC | 3 cut(s) | 542, 1427, 1559 |
| BanII | GRGCYC | 3 cut(s) | 315, 1694, 2069 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 1129, 1161, 2156, 2188 |
| BauI | CACGAG | 2 cut(s) | 502, 922 |
| BbrPI | CACGTG | 3 cut(s) | 489, 1645, 1813 |
| BbsI | GAAGAC | 1 cut(s) | 2013 |
| Bbv12I | GWGCWC | 4 cut(s) | 92, 141, 315, 929 |
| BbvCI | CCTCAGC | 1 cut(s) | 1031 |
| BbvI | GCAGC | 4 cut(s) | 247, 1320, 1650, 2226 |
| BceAI | ACGGC | 7 cut(s) | 238, 643, 1318, 1359, 1378, 1422, 1749 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1417, 1451, 1784, 1818 |
| BciT130I | CCWGG | 4 cut(s) | 547, 607, 733, 1530 |
| BcoDI | GTCTC | 2 cut(s) | 251, 612 |
| BfaI | CTAG | 4 cut(s) | 12, 75, 681, 1007 |
| BfoI | RGCGCY | 2 cut(s) | 1563, 1715 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1733 |
| BmcAI | AGTACT | 1 cut(s) | 2042 |
| BmeT110I | CYCGRG | 7 cut(s) | 275, 296, 332, 1230, 1476, 2044, 2228 |
| BmgBI | CACGTC | 2 cut(s) | 1626, 1765 |
| BmsI | GCATC | 1 cut(s) | 183 |
| BmtI | GCTAGC | 1 cut(s) | 684 |
| BpiI | GAAGAC | 1 cut(s) | 2013 |
| BpmI | CTGGAG | 1 cut(s) | 438 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1031 |
| BpuEI | CTTGAG | 2 cut(s) | 248, 1652 |
| BsaAI | YACGTR | 3 cut(s) | 489, 1645, 1813 |
| BsaBI | GATNNNNATC | 3 cut(s) | 917, 1398, 2270 |
| BsaHI | GRCGYC | 5 cut(s) | 242, 393, 450, 1109, 1560 |
| BsaWI | WCCGGW | 4 cut(s) | 674, 1453, 1574, 1772 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 212, 242, 593, 623, 1601, 1631, 2120, 2150 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 277, 376, 660, 714, 1423, 1600, 1825, 1837 |
| Bse118I | RCCGGY | 4 cut(s) | 124, 1124, 1336, 1574 |
| Bse1I | ACTGG | 4 cut(s) | 155, 387, 421, 1134 |
| Bse8I | GATNNNNATC | 3 cut(s) | 917, 1398, 2270 |
| BseBI | CCWGG | 4 cut(s) | 547, 607, 733, 1530 |
| BseGI | GGATG | 4 cut(s) | 216, 723, 1781, 2107 |
| BseJI | GATNNNNATC | 3 cut(s) | 917, 1398, 2270 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 277, 376, 660, 714, 1423, 1600, 1825, 1837 |
| BseMII | CTCAG | 2 cut(s) | 1045, 1071 |
| BseNI | ACTGG | 4 cut(s) | 155, 387, 421, 1134 |
| BseRI | GAGGAG | 3 cut(s) | 263, 505, 1322 |
| BseSI | GKGCMC | 4 cut(s) | 488, 1004, 1430, 2069 |
| BseX3I | CGGCCG | 4 cut(s) | 186, 930, 1407, 2070 |
| BseXI | GCAGC | 4 cut(s) | 247, 1320, 1650, 2226 |
| BseYI | CCCAGC | 1 cut(s) | 947 |
| Bsh1285I | CGRYCG | 8 cut(s) | 189, 885, 933, 1216, 1410, 1587, 1709, 2073 |
| BshNI | GGYRCC | 3 cut(s) | 542, 1427, 1559 |
| BshTI | ACCGGT | 1 cut(s) | 1574 |
| BsiEI | CGRYCG | 8 cut(s) | 189, 885, 933, 1216, 1410, 1587, 1709, 2073 |
| BsiHKAI | GWGCWC | 4 cut(s) | 92, 141, 315, 929 |
| BsiHKCI | CYCGRG | 7 cut(s) | 275, 296, 332, 1230, 1476, 2044, 2228 |
| BslFI | GGGAC | 3 cut(s) | 595, 884, 930 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 277, 376, 660, 714, 1423, 1600, 1825, 1837 |
| BsmAI | GTCTC | 2 cut(s) | 251, 612 |
| BsmBI | CGTCTC | 2 cut(s) | 251, 612 |
| BsmFI | GGGAC | 3 cut(s) | 595, 884, 930 |
| BsoBI | CYCGRG | 7 cut(s) | 275, 296, 332, 1230, 1476, 2044, 2228 |
| Bsp120I | GGGCCC | 1 cut(s) | 2065 |
| Bsp1286I | GDGCHC | 9 cut(s) | 92, 141, 315, 488, 929, 1004, 1430, 1694, 2069 |
| Bsp19I | CCATGG | 1 cut(s) | 2097 |
| Bsp68I | TCGCGA | 1 cut(s) | 1014 |
| BspCNI | CTCAG | 2 cut(s) | 1044, 1070 |
| BspOI | GCTAGC | 1 cut(s) | 684 |
| BspQI | GCTCTTC | 1 cut(s) | 371 |
| BspT107I | GGYRCC | 3 cut(s) | 542, 1427, 1559 |
| BsrBI | CCGCTC | 2 cut(s) | 1650, 2010 |
| BsrFI | RCCGGY | 4 cut(s) | 124, 1124, 1336, 1574 |
| BsrI | ACTGG | 4 cut(s) | 155, 387, 421, 1134 |
| BssAI | RCCGGY | 4 cut(s) | 124, 1124, 1336, 1574 |
| BssNI | GRCGYC | 5 cut(s) | 242, 393, 450, 1109, 1560 |
| BssSI | CACGAG | 2 cut(s) | 502, 922 |
| BssT1I | CCWWGG | 3 cut(s) | 597, 1431, 2097 |
| Bst2BI | CACGAG | 2 cut(s) | 502, 922 |
| Bst2UI | CCWGG | 4 cut(s) | 547, 607, 733, 1530 |
| Bst4CI | ACNGT | 9 cut(s) | 959, 991, 1046, 1217, 1249, 1802, 1861, 2032, 2179 |
| Bst6I | CTCTTC | 1 cut(s) | 371 |
| BstACI | GRCGYC | 5 cut(s) | 242, 393, 450, 1109, 1560 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1756 |
| BstBAI | YACGTR | 3 cut(s) | 489, 1645, 1813 |
| BstC8I | GCNNGC | 8 cut(s) | 126, 475, 649, 682, 729, 1000, 1338, 1942 |
| BstDEI | CTNAG | 3 cut(s) | 1031, 1057, 1543 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1421 |
| BstF5I | GGATG | 4 cut(s) | 216, 723, 1781, 2107 |
| BstH2I | RGCGCY | 2 cut(s) | 1563, 1715 |
| BstHHI | GCGC | 9 cut(s) | 700, 712, 953, 998, 1528, 1562, 1599, 1663, 1714 |
| BstMAI | GTCTC | 2 cut(s) | 251, 612 |
| BstMCI | CGRYCG | 8 cut(s) | 189, 885, 933, 1216, 1410, 1587, 1709, 2073 |
| BstNI | CCWGG | 4 cut(s) | 547, 607, 733, 1530 |
| BstSLI | GKGCMC | 4 cut(s) | 488, 1004, 1430, 2069 |
| BstV1I | GCAGC | 4 cut(s) | 247, 1320, 1650, 2226 |
| BstV2I | GAAGAC | 1 cut(s) | 2013 |
| BstX2I | RGATCY | 5 cut(s) | 704, 887, 912, 1393, 1972 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1099, 1579 |
| BstYI | RGATCY | 5 cut(s) | 704, 887, 912, 1393, 1972 |
| BstZI | CGGCCG | 4 cut(s) | 186, 930, 1407, 2070 |
| BtgZI | GCGATG | 2 cut(s) | 914, 1380 |
| BtrI | CACGTC | 2 cut(s) | 1626, 1765 |
| BtsCI | GGATG | 4 cut(s) | 216, 723, 1781, 2107 |
| BtsIMutI | CAGTG | 4 cut(s) | 148, 394, 414, 2028 |
| BtuMI | TCGCGA | 1 cut(s) | 1014 |
| Cac8I | GCNNGC | 8 cut(s) | 126, 475, 649, 682, 729, 1000, 1338, 1942 |
| CfoI | GCGC | 9 cut(s) | 700, 712, 953, 998, 1528, 1562, 1599, 1663, 1714 |
| Cfr10I | RCCGGY | 4 cut(s) | 124, 1124, 1336, 1574 |
| Cfr42I | CCGCGG | 4 cut(s) | 54, 657, 1078, 2154 |
| Cfr9I | CCCGGG | 2 cut(s) | 275, 1230 |
| CseI | GACGC | 8 cut(s) | 80, 150, 167, 250, 268, 458, 1117, 1331 |
| Csp6I | GTAC | 6 cut(s) | 154, 307, 550, 955, 2041, 2080 |
| CspAI | ACCGGT | 1 cut(s) | 1574 |
| CviAII | CATG | 6 cut(s) | 7, 106, 835, 1162, 1276, 2098 |
| CviQI | GTAC | 6 cut(s) | 154, 307, 550, 955, 2041, 2080 |
| DdeI | CTNAG | 3 cut(s) | 1031, 1057, 1543 |
| DinI | GGCGCC | 1 cut(s) | 1561 |
| DrdI | GACNNNNNNGTC | 4 cut(s) | 398, 419, 818, 1220 |
| DseDI | GACNNNNNNGTC | 4 cut(s) | 398, 419, 818, 1220 |
| EagI | CGGCCG | 4 cut(s) | 186, 930, 1407, 2070 |
| Eam1104I | CTCTTC | 1 cut(s) | 371 |
| EarI | CTCTTC | 1 cut(s) | 371 |
| EciI | GGCGGA | 3 cut(s) | 71, 83, 864 |
| Ecl136II | GAGCTC | 1 cut(s) | 313 |
| EclXI | CGGCCG | 4 cut(s) | 186, 930, 1407, 2070 |
| Eco130I | CCWWGG | 3 cut(s) | 597, 1431, 2097 |
| Eco24I | GRGCYC | 3 cut(s) | 315, 1694, 2069 |
| Eco52I | CGGCCG | 4 cut(s) | 186, 930, 1407, 2070 |
| Eco53kI | GAGCTC | 1 cut(s) | 313 |
| Eco72I | CACGTG | 3 cut(s) | 489, 1645, 1813 |
| Eco88I | CYCGRG | 7 cut(s) | 275, 296, 332, 1230, 1476, 2044, 2228 |
| EcoICRI | GAGCTC | 1 cut(s) | 313 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1421 |
| EcoO109I | RGGNCCY | 3 cut(s) | 581, 609, 944 |
| EcoRII | CCWGG | 4 cut(s) | 545, 605, 731, 1528 |
| EcoT14I | CCWWGG | 3 cut(s) | 597, 1431, 2097 |
| EcoT38I | GRGCYC | 3 cut(s) | 315, 1694, 2069 |
| EgeI | GGCGCC | 1 cut(s) | 1561 |
| EheI | GGCGCC | 1 cut(s) | 1561 |
| ErhI | CCWWGG | 3 cut(s) | 597, 1431, 2097 |
| Esp3I | CGTCTC | 2 cut(s) | 251, 612 |
| FaeI | CATG | 6 cut(s) | 10, 109, 838, 1165, 1279, 2101 |
| FaiI | YATR | 7 cut(s) | 8, 107, 797, 836, 1163, 1277, 2099 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 899, 931 |
| FaqI | GGGAC | 3 cut(s) | 595, 884, 930 |
| FatI | CATG | 6 cut(s) | 6, 105, 834, 1161, 1275, 2097 |
| FauI | CCCGC | 5 cut(s) | 652, 758, 934, 991, 1702 |
| FblI | GTMKAC | 2 cut(s) | 1587, 1848 |
| FokI | GGATG | 4 cut(s) | 203, 730, 1768, 2114 |
| FriOI | GRGCYC | 3 cut(s) | 315, 1694, 2069 |
| FspBI | CTAG | 4 cut(s) | 12, 75, 681, 1007 |
| GlaI | GCGC | 9 cut(s) | 699, 711, 952, 997, 1527, 1561, 1598, 1662, 1713 |
| GsaI | CCCAGC | 1 cut(s) | 951 |
| GsuI | CTGGAG | 1 cut(s) | 438 |
| HaeII | RGCGCY | 2 cut(s) | 1563, 1715 |
| HgaI | GACGC | 8 cut(s) | 80, 150, 167, 250, 268, 458, 1117, 1331 |
| HhaI | GCGC | 9 cut(s) | 700, 712, 953, 998, 1528, 1562, 1599, 1663, 1714 |
| Hin1I | GRCGYC | 5 cut(s) | 242, 393, 450, 1109, 1560 |
| Hin1II | CATG | 6 cut(s) | 10, 109, 838, 1165, 1279, 2101 |
| Hin6I | GCGC | 9 cut(s) | 698, 710, 951, 996, 1526, 1560, 1597, 1661, 1712 |
| HinP1I | GCGC | 9 cut(s) | 698, 710, 951, 996, 1526, 1560, 1597, 1661, 1712 |
| HincII | GTYRAC | 4 cut(s) | 823, 1225, 1537, 1588 |
| HindII | GTYRAC | 4 cut(s) | 823, 1225, 1537, 1588 |
| HphI | GGTGA | 7 cut(s) | 664, 1201, 1264, 2017, 2047, 2125, 2191 |
| Hpy166II | GTNNAC | 9 cut(s) | 103, 823, 1225, 1537, 1588, 1678, 1816, 1849, 2028 |
| Hpy188III | TCNNGA | 8 cut(s) | 19, 296, 787, 916, 1013, 1237, 1476, 2202 |
| Hpy8I | GTNNAC | 9 cut(s) | 103, 823, 1225, 1537, 1588, 1678, 1816, 1849, 2028 |
| HpyAV | CCTTC | 7 cut(s) | 475, 901, 1186, 1458, 1801, 1995, 2161 |
| HpyCH4III | ACNGT | 9 cut(s) | 959, 991, 1046, 1217, 1249, 1802, 1861, 2032, 2179 |
| HpyCH4V | TGCA | 2 cut(s) | 1161, 1759 |
| HpyF3I | CTNAG | 3 cut(s) | 1031, 1057, 1543 |
| Hsp92I | GRCGYC | 5 cut(s) | 242, 393, 450, 1109, 1560 |
| Hsp92II | CATG | 6 cut(s) | 10, 109, 838, 1165, 1279, 2101 |
| HspAI | GCGC | 9 cut(s) | 698, 710, 951, 996, 1526, 1560, 1597, 1661, 1712 |
| KasI | GGCGCC | 1 cut(s) | 1559 |
| KflI | GGGWCCC | 1 cut(s) | 944 |
| KroI | GCCGGC | 2 cut(s) | 124, 1336 |
| KroNI | GCCGGC | 2 cut(s) | 126, 1338 |
| KspI | CCGCGG | 4 cut(s) | 54, 657, 1078, 2154 |
| LguI | GCTCTTC | 1 cut(s) | 371 |
| LmnI | GCTCC | 9 cut(s) | 87, 318, 677, 782, 979, 1309, 1377, 1689, 2128 |
| Lsp1109I | GCAGC | 4 cut(s) | 247, 1320, 1650, 2226 |
| LweI | GCATC | 1 cut(s) | 183 |
| MaeI | CTAG | 4 cut(s) | 12, 75, 681, 1007 |
| MbiI | CCGCTC | 2 cut(s) | 1650, 2010 |
| MboII | GAAGA | 6 cut(s) | 275, 284, 358, 2013, 2128, 2176 |
| MflI | RGATCY | 5 cut(s) | 704, 887, 912, 1393, 1972 |
| MhlI | GDGCHC | 9 cut(s) | 92, 141, 315, 488, 929, 1004, 1430, 1694, 2069 |
| MlsI | TGGCCA | 4 cut(s) | 5, 427, 722, 794 |
| MluCI | AATT | 2 cut(s) | 1081, 1999 |
| MluI | ACGCGT | 1 cut(s) | 159 |
| MluNI | TGGCCA | 4 cut(s) | 5, 427, 722, 794 |
| Mly113I | GGCGCC | 1 cut(s) | 1560 |
| Mox20I | TGGCCA | 4 cut(s) | 5, 427, 722, 794 |
| MroNI | GCCGGC | 2 cut(s) | 124, 1336 |
| MscI | TGGCCA | 4 cut(s) | 5, 427, 722, 794 |
| MslI | CAYNNNNRTG | 3 cut(s) | 788, 1628, 2060 |
| Msp20I | TGGCCA | 4 cut(s) | 5, 427, 722, 794 |
| MspA1I | CMGCKG | 5 cut(s) | 53, 238, 656, 1077, 2153 |
| MvaI | CCWGG | 4 cut(s) | 547, 607, 733, 1530 |
| NaeI | GCCGGC | 2 cut(s) | 126, 1338 |
| NarI | GGCGCC | 1 cut(s) | 1560 |
| NcoI | CCATGG | 1 cut(s) | 2097 |
| NgoMIV | GCCGGC | 2 cut(s) | 124, 1336 |
| NheI | GCTAGC | 1 cut(s) | 680 |
| NlaIII | CATG | 6 cut(s) | 10, 109, 838, 1165, 1279, 2101 |
| NmeAIII | GCCGAG | 8 cut(s) | 164, 214, 280, 497, 822, 875, 908, 950 |
| NmuCI | GTSAC | 3 cut(s) | 389, 670, 2179 |
| NruI | TCGCGA | 1 cut(s) | 1014 |
| OliI | CACNNNNGTG | 1 cut(s) | 2060 |
| PaeR7I | CTCGAG | 2 cut(s) | 2044, 2228 |
| PciSI | GCTCTTC | 1 cut(s) | 371 |
| PcsI | WCGNNNNNNNCGW | 6 cut(s) | 294, 816, 1368, 1818, 2021, 2084 |
| PdiI | GCCGGC | 2 cut(s) | 126, 1338 |
| PfeI | GAWTC | 4 cut(s) | 1153, 1269, 1399, 1440 |
| PflFI | GACNNNGTC | 2 cut(s) | 818, 1520 |
| PfoI | TCCNGGA | 1 cut(s) | 1148 |
| PinAI | ACCGGT | 1 cut(s) | 1574 |
| Ple19I | CGATCG | 2 cut(s) | 885, 1709 |
| PluTI | GGCGCC | 1 cut(s) | 1563 |
| PmaCI | CACGTG | 3 cut(s) | 489, 1645, 1813 |
| PmlI | CACGTG | 3 cut(s) | 489, 1645, 1813 |
| Ppu21I | YACGTR | 3 cut(s) | 489, 1645, 1813 |
| PpuMI | RGGWCCY | 2 cut(s) | 609, 944 |
| Psp124BI | GAGCTC | 1 cut(s) | 315 |
| Psp1406I | AACGTT | 1 cut(s) | 825 |
| Psp5II | RGGWCCY | 2 cut(s) | 609, 944 |
| Psp6I | CCWGG | 4 cut(s) | 545, 605, 731, 1528 |
| PspCI | CACGTG | 3 cut(s) | 489, 1645, 1813 |
| PspFI | CCCAGC | 1 cut(s) | 947 |
| PspGI | CCWGG | 4 cut(s) | 545, 605, 731, 1528 |
| PspOMI | GGGCCC | 1 cut(s) | 2065 |
| PspPPI | RGGWCCY | 2 cut(s) | 609, 944 |
| PspXI | VCTCGAGB | 2 cut(s) | 2044, 2228 |
| PsuI | RGATCY | 5 cut(s) | 704, 887, 912, 1393, 1972 |
| PsyI | GACNNNGTC | 2 cut(s) | 818, 1520 |
| PvuI | CGATCG | 2 cut(s) | 885, 1709 |
| PvuII | CAGCTG | 1 cut(s) | 238 |
| RruI | TCGCGA | 1 cut(s) | 1014 |
| RsaI | GTAC | 6 cut(s) | 155, 308, 551, 956, 2042, 2081 |
| RsaNI | GTAC | 6 cut(s) | 154, 307, 550, 955, 2041, 2080 |
| RseI | CAYNNNNRTG | 3 cut(s) | 788, 1628, 2060 |
| SacI | GAGCTC | 1 cut(s) | 315 |
| SacII | CCGCGG | 4 cut(s) | 54, 657, 1078, 2154 |
| SalI | GTCGAC | 1 cut(s) | 1586 |
| SapI | GCTCTTC | 1 cut(s) | 371 |
| ScaI | AGTACT | 1 cut(s) | 2042 |
| SduI | GDGCHC | 9 cut(s) | 92, 141, 315, 488, 929, 1004, 1430, 1694, 2069 |
| SfaNI | GCATC | 1 cut(s) | 183 |
| SfoI | GGCGCC | 1 cut(s) | 1561 |
| Sfr274I | CTCGAG | 2 cut(s) | 2044, 2228 |
| Sfr303I | CCGCGG | 4 cut(s) | 54, 657, 1078, 2154 |
| SgrBI | CCGCGG | 4 cut(s) | 54, 657, 1078, 2154 |
| SlaI | CTCGAG | 2 cut(s) | 2044, 2228 |
| SmaI | CCCGGG | 2 cut(s) | 277, 1232 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 788, 1628, 2060 |
| SmlI | CTYRAG | 4 cut(s) | 263, 1667, 2044, 2228 |
| SmoI | CTYRAG | 4 cut(s) | 263, 1667, 2044, 2228 |
| Sse9I | AATT | 2 cut(s) | 1081, 1999 |
| SspDI | GGCGCC | 1 cut(s) | 1559 |
| SspMI | CTAG | 4 cut(s) | 12, 75, 681, 1007 |
| SstI | GAGCTC | 1 cut(s) | 315 |
| StyI | CCWWGG | 3 cut(s) | 597, 1431, 2097 |
| TaaI | ACNGT | 9 cut(s) | 959, 991, 1046, 1217, 1249, 1802, 1861, 2032, 2179 |
| TasI | AATT | 2 cut(s) | 1081, 1999 |
| TatI | WGTACW | 1 cut(s) | 2040 |
| TfiI | GAWTC | 4 cut(s) | 1153, 1269, 1399, 1440 |
| TscAI | CASTG | 4 cut(s) | 155, 394, 421, 2035 |
| TseFI | GTSAC | 3 cut(s) | 389, 670, 2179 |
| TseI | GCWGC | 4 cut(s) | 235, 1333, 1663, 2214 |
| Tsp45I | GTSAC | 3 cut(s) | 389, 670, 2179 |
| TspGWI | ACGGA | 3 cut(s) | 648, 655, 966 |
| TspMI | CCCGGG | 2 cut(s) | 275, 1230 |
| TspRI | CASTG | 4 cut(s) | 155, 394, 421, 2035 |
| Tth111I | GACNNNGTC | 2 cut(s) | 818, 1520 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1421 |
| XapI | RAATTY | 1 cut(s) | 1999 |
| XhoI | CTCGAG | 2 cut(s) | 2044, 2228 |
| XmaI | CCCGGG | 2 cut(s) | 275, 1230 |
| XmiI | GTMKAC | 2 cut(s) | 1587, 1848 |
| XspI | CTAG | 4 cut(s) | 12, 75, 681, 1007 |
| ZraI | GACGTC | 1 cut(s) | 394 |
| ZrmI | AGTACT | 1 cut(s) | 2042 |