FvH4_3g14600

Knottins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001621
Physical Location & Seq
Forward (+)
9004132 .. 9006668
2537 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g14600.t2

Sequence Viewer

Length: 225 bp
ATGGAGAGGAAGTACTTCGGGTTTGCTTTGTTCTTCATCATCCTCTTAACTTCTCAAGAGATGGTGATTCAGAGTGAAGCAAGAGTCTGCGAAGTGCCAAGCAAGACCTTCAAGGGGATGTGCTTCAGAGACACCCACTGCATTGTGAGATGCCACAGTGAGGGTTACGGTTACGGCAAATGCTCTCATGTCCTGCGACGGTGCAAATGCTTGAAGGCTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

75

Amino Acids

8.59

Weight (kDa)

9.03

Isoelectric Point (pI)

45.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 29 - 74 2e-14 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 109
AfaI GTAC 1 cut(s) 14
AfiI CCNNNNNNNGG 2 cut(s) 114, 160
AgsI TTSAA 2 cut(s) 112, 214
Alw26I GTCTC 1 cut(s) 123
Asp700I GAANNNNTTC 1 cut(s) 14
AsuHPI GGTGA 1 cut(s) 76
BccI CCATC 1 cut(s) 55
BceAI ACGGC 1 cut(s) 190
BcoDI GTCTC 1 cut(s) 123
BfaI CTAG 1 cut(s) 223
BmcAI AGTACT 1 cut(s) 14
BmsI GCATC 1 cut(s) 140
BpuEI CTTGAG 1 cut(s) 39
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 114, 160
BseGI GGATG 2 cut(s) 39, 123
BseLI CCNNNNNNNGG 2 cut(s) 114, 160
BslI CCNNNNNNNGG 2 cut(s) 114, 160
BsmAI GTCTC 1 cut(s) 123
Bst4CI ACNGT 3 cut(s) 158, 170, 201
BstC8I GCNNGC 1 cut(s) 220
BstF5I GGATG 2 cut(s) 39, 123
BstMAI GTCTC 1 cut(s) 123
BtsCI GGATG 2 cut(s) 39, 123
BtsI GCAGTG 1 cut(s) 136
BtsIMutI CAGTG 2 cut(s) 136, 163
Cac8I GCNNGC 1 cut(s) 220
Csp6I GTAC 1 cut(s) 13
CviAII CATG 1 cut(s) 188
CviJI RGCY 1 cut(s) 218
CviKI_1 RGCY 1 cut(s) 218
CviQI GTAC 1 cut(s) 13
Eco57I CTGAAG 1 cut(s) 109
FaeI CATG 1 cut(s) 191
FaiI YATR 1 cut(s) 189
FatI CATG 1 cut(s) 187
FokI GGATG 2 cut(s) 26, 130
FspBI CTAG 1 cut(s) 223
Hin1II CATG 1 cut(s) 191
HinfI GANTC 2 cut(s) 67, 84
HphI GGTGA 1 cut(s) 76
Hpy188I TCNGA 2 cut(s) 72, 128
Hpy188III TCNNGA 1 cut(s) 56
Hpy99I CGWCG 1 cut(s) 201
HpyAV CCTTC 2 cut(s) 118, 208
HpyCH4III ACNGT 3 cut(s) 158, 170, 201
HpyCH4V TGCA 2 cut(s) 141, 204
Hsp92II CATG 1 cut(s) 191
LpnPI CCDG 1 cut(s) 206
LweI GCATC 1 cut(s) 140
MaeI CTAG 1 cut(s) 223
MaeIII GTNAC 2 cut(s) 164, 170
MboII GAAGA 1 cut(s) 25
MlyI GAGTC 1 cut(s) 93
MnlI CCTC 2 cut(s) 53, 154
MroXI GAANNNNTTC 1 cut(s) 14
MseI TTAA 1 cut(s) 47
NlaIII CATG 1 cut(s) 191
PdmI GAANNNNTTC 1 cut(s) 14
PfeI GAWTC 1 cut(s) 67
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
RsaI GTAC 1 cut(s) 14
RsaNI GTAC 1 cut(s) 13
SaqAI TTAA 1 cut(s) 47
ScaI AGTACT 1 cut(s) 14
SchI GAGTC 1 cut(s) 93
SetI ASST 1 cut(s) 110
SfaNI GCATC 1 cut(s) 140
SgeI CNNG 8 cut(s) 31, 68, 93, 111, 115, 124, 200, 205
SmlI CTYRAG 1 cut(s) 54
SmoI CTYRAG 1 cut(s) 54
SspMI CTAG 1 cut(s) 223
TaaI ACNGT 3 cut(s) 158, 170, 201
TatI WGTACW 1 cut(s) 12
TfiI GAWTC 1 cut(s) 67
Tru1I TTAA 1 cut(s) 47
Tru9I TTAA 1 cut(s) 47
TscAI CASTG 2 cut(s) 143, 163
TspDTI ATGAA 1 cut(s) 25
TspRI CASTG 2 cut(s) 143, 163
XmnI GAANNNNTTC 1 cut(s) 14
XspI CTAG 1 cut(s) 223
ZrmI AGTACT 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.