FvH4_5g33001

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001621
Physical Location & Seq
Reverse (-)
23837046 .. 23837378
333 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g33001.t1

Sequence Viewer

Length: 228 bp
ATGATGGGGAGGAAATGTCTGGTTGTTTTCTTCTTGCTCTTCATTGTTCTCGCTTCACAAGATATGATAGTGCCAAGTGAGGCTAGGGTTTGCGAGTCGCAGAGTCATGGTTTCCATGGGACATGCATTCGCGCCCATAACTGTGCTCTCGTCTGCAGGCACGAGGGTTTCTCCGGTGGCAGATGCCGCGGCTTCCGGCGCCGATGCTTTTGCACTCGAAGATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

76

Amino Acids

8.56

Weight (kDa)

9.48

Isoelectric Point (pI)

61.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 29 - 75 2.1e-17 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 198
AccII CGCG 2 cut(s) 132, 189
AciI CCGC 2 cut(s) 187, 189
AcyI GRCGYC 1 cut(s) 199
Alw21I GWGCWC 1 cut(s) 148
AspLEI GCGC 2 cut(s) 134, 201
BanI GGYRCC 1 cut(s) 198
BauI CACGAG 1 cut(s) 161
Bbv12I GWGCWC 1 cut(s) 148
BcgI CGANNNNNNTGC 2 cut(s) 192, 226
BfaI CTAG 1 cut(s) 84
BfmI CTRYAG 1 cut(s) 154
BfoI RGCGCY 1 cut(s) 202
BisI GCNGC 2 cut(s) 187, 190
BlsI GCNGC 2 cut(s) 188, 191
BmiI GGNNCC 1 cut(s) 200
BmsI GCATC 2 cut(s) 173, 194
BplI GAGNNNNNCTC 2 cut(s) 155, 187
BsaHI GRCGYC 1 cut(s) 199
BsaJI CCNNGG 2 cut(s) 115, 187
BsaWI WCCGGW 1 cut(s) 173
BsaXI ACNNNNNCTCC 1 cut(s) 31
BseDI CCNNGG 2 cut(s) 115, 187
Bsh1236I CGCG 2 cut(s) 132, 189
BshNI GGYRCC 1 cut(s) 198
BsiHKAI GWGCWC 1 cut(s) 148
BsiSI CCGG 2 cut(s) 174, 196
BslFI GGGAC 1 cut(s) 133
BsmFI GGGAC 1 cut(s) 133
BsmI GAATGC 1 cut(s) 126
Bsp1286I GDGCHC 1 cut(s) 148
Bsp19I CCATGG 1 cut(s) 115
BspACI CCGC 2 cut(s) 187, 189
BspFNI CGCG 2 cut(s) 132, 189
BspLI GGNNCC 1 cut(s) 200
BspMAI CTGCAG 1 cut(s) 158
BspQI GCTCTTC 1 cut(s) 44
BspT107I GGYRCC 1 cut(s) 198
BssECI CCNNGG 2 cut(s) 115, 187
BssNI GRCGYC 1 cut(s) 199
BssSI CACGAG 1 cut(s) 161
BssT1I CCWWGG 1 cut(s) 115
Bst2BI CACGAG 1 cut(s) 161
Bst4CI ACNGT 1 cut(s) 143
Bst6I CTCTTC 1 cut(s) 44
BstACI GRCGYC 1 cut(s) 199
BstC8I GCNNGC 1 cut(s) 158
BstDSI CCRYGG 2 cut(s) 115, 187
BstFNI CGCG 2 cut(s) 132, 189
BstH2I RGCGCY 1 cut(s) 202
BstHHI GCGC 2 cut(s) 134, 201
BstMWI GCNNNNNNNGC 2 cut(s) 186, 198
BstNSI RCATGY 1 cut(s) 126
BstSFI CTRYAG 1 cut(s) 154
BstUI CGCG 2 cut(s) 132, 189
BtgI CCRYGG 2 cut(s) 115, 187
Cac8I GCNNGC 1 cut(s) 158
CfoI GCGC 2 cut(s) 134, 201
Cfr42I CCGCGG 1 cut(s) 190
CviAII CATG 3 cut(s) 107, 116, 123
CviJI RGCY 2 cut(s) 83, 192
CviKI_1 RGCY 2 cut(s) 83, 192
DinI GGCGCC 1 cut(s) 200
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
Eco130I CCWWGG 1 cut(s) 115
EcoT14I CCWWGG 1 cut(s) 115
EcoT22I ATGCAT 1 cut(s) 128
EgeI GGCGCC 1 cut(s) 200
EheI GGCGCC 1 cut(s) 200
ErhI CCWWGG 1 cut(s) 115
FaeI CATG 3 cut(s) 110, 119, 126
FaiI YATR 5 cut(s) 65, 108, 117, 124, 138
FaqI GGGAC 1 cut(s) 133
FatI CATG 3 cut(s) 106, 115, 122
Fnu4HI GCNGC 2 cut(s) 187, 190
Fsp4HI GCNGC 2 cut(s) 187, 190
FspBI CTAG 1 cut(s) 84
GlaI GCGC 2 cut(s) 133, 200
GluI GCNGC 2 cut(s) 187, 190
HaeII RGCGCY 1 cut(s) 202
HapII CCGG 2 cut(s) 174, 196
HhaI GCGC 2 cut(s) 134, 201
Hin1I GRCGYC 1 cut(s) 199
Hin1II CATG 3 cut(s) 110, 119, 126
Hin6I GCGC 2 cut(s) 132, 199
HinP1I GCGC 2 cut(s) 132, 199
HinfI GANTC 2 cut(s) 95, 103
HpaII CCGG 2 cut(s) 174, 196
HpyCH4III ACNGT 1 cut(s) 143
HpyCH4V TGCA 3 cut(s) 126, 156, 213
HpyF10VI GCNNNNNNNGC 2 cut(s) 186, 198
Hsp92I GRCGYC 1 cut(s) 199
Hsp92II CATG 3 cut(s) 110, 119, 126
HspAI GCGC 2 cut(s) 132, 199
KasI GGCGCC 1 cut(s) 198
KspI CCGCGG 1 cut(s) 190
LguI GCTCTTC 1 cut(s) 44
LpnPI CCDG 4 cut(s) 5, 142, 187, 209
LweI GCATC 2 cut(s) 173, 194
MaeI CTAG 1 cut(s) 84
MboII GAAGA 2 cut(s) 22, 31
MhlI GDGCHC 1 cut(s) 148
Mly113I GGCGCC 1 cut(s) 199
MlyI GAGTC 2 cut(s) 104, 112
MnlI CCTC 3 cut(s) 3, 73, 157
Mph1103I ATGCAT 1 cut(s) 128
MslI CAYNNNNRTG 1 cut(s) 141
MspA1I CMGCKG 1 cut(s) 189
MspI CCGG 2 cut(s) 174, 196
Mva1269I GAATGC 1 cut(s) 126
MvnI CGCG 2 cut(s) 132, 189
MwoI GCNNNNNNNGC 2 cut(s) 186, 198
NarI GGCGCC 1 cut(s) 199
NcoI CCATGG 1 cut(s) 115
NlaIII CATG 3 cut(s) 110, 119, 126
NlaIV GGNNCC 1 cut(s) 200
NsiI ATGCAT 1 cut(s) 128
NspI RCATGY 1 cut(s) 126
PciSI GCTCTTC 1 cut(s) 44
PctI GAATGC 1 cut(s) 126
PkrI GCNGC 2 cut(s) 188, 191
PleI GAGTC 2 cut(s) 103, 111
PluTI GGCGCC 1 cut(s) 202
PpsI GAGTC 2 cut(s) 103, 111
PspN4I GGNNCC 1 cut(s) 200
PstI CTGCAG 1 cut(s) 158
RseI CAYNNNNRTG 1 cut(s) 141
SacII CCGCGG 1 cut(s) 190
SapI GCTCTTC 1 cut(s) 44
SatI GCNGC 2 cut(s) 187, 190
SchI GAGTC 2 cut(s) 104, 112
SduI GDGCHC 1 cut(s) 148
SfaNI GCATC 2 cut(s) 173, 194
SfcI CTRYAG 1 cut(s) 154
SfoI GGCGCC 1 cut(s) 200
Sfr303I CCGCGG 1 cut(s) 190
SgrBI CCGCGG 1 cut(s) 190
SmiMI CAYNNNNRTG 1 cut(s) 141
SsiI CCGC 2 cut(s) 187, 189
SspDI GGCGCC 1 cut(s) 198
SspMI CTAG 1 cut(s) 84
StyI CCWWGG 1 cut(s) 115
TaaI ACNGT 1 cut(s) 143
TaqI TCGA 1 cut(s) 217
TauI GCSGC 2 cut(s) 189, 192
TspDTI ATGAA 1 cut(s) 31
XceI RCATGY 1 cut(s) 126
XspI CTAG 1 cut(s) 84
Zsp2I ATGCAT 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.