Rw0G010770

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00464
OG0001621
Physical Location & Seq
Forward (+)
7565 .. 8720
1156 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G010770.1

Sequence Viewer

Length: 222 bp
ATGAAATTGAAATTAAAATTGATGGCTTTTGCTTTTGCATTTGCTCTTATCATTTTGGTGTTGCTTACAAATGAGGCTAGGGTTTGCGAGTCACAGAGTCATGGATTCCATGGGACATGCATTCGCGCCCACAACTGTGCTCTCGTCTGCAGGCACGAGGGTTTCTCTGGTGGCAGATGCCGCGGCTTCCGGCGCCGATGCTTTTGCACTCGAAGATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

73

Amino Acids

8.32

Weight (kDa)

10.04

Isoelectric Point (pI)

46.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 27 - 73 1.9e-17 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 192
AccII CGCG 2 cut(s) 126, 183
AciI CCGC 2 cut(s) 181, 183
AcyI GRCGYC 1 cut(s) 193
AgsI TTSAA 1 cut(s) 10
AleI CACNNNNGTG 1 cut(s) 135
Alw21I GWGCWC 1 cut(s) 142
AspLEI GCGC 2 cut(s) 128, 195
BanI GGYRCC 1 cut(s) 192
BauI CACGAG 1 cut(s) 155
Bbv12I GWGCWC 1 cut(s) 142
BccI CCATC 1 cut(s) 16
BcgI CGANNNNNNTGC 2 cut(s) 186, 220
BfaI CTAG 1 cut(s) 78
BfmI CTRYAG 1 cut(s) 148
BfoI RGCGCY 1 cut(s) 196
BisI GCNGC 2 cut(s) 181, 184
BlsI GCNGC 2 cut(s) 182, 185
BmiI GGNNCC 1 cut(s) 194
BmsI GCATC 2 cut(s) 167, 188
BplI GAGNNNNNCTC 2 cut(s) 149, 181
BsaHI GRCGYC 1 cut(s) 193
BsaJI CCNNGG 2 cut(s) 109, 181
BseDI CCNNGG 2 cut(s) 109, 181
Bsh1236I CGCG 2 cut(s) 126, 183
BshNI GGYRCC 1 cut(s) 192
BsiHKAI GWGCWC 1 cut(s) 142
BsiSI CCGG 1 cut(s) 190
BslFI GGGAC 1 cut(s) 127
BsmFI GGGAC 1 cut(s) 127
BsmI GAATGC 1 cut(s) 120
Bsp1286I GDGCHC 1 cut(s) 142
Bsp19I CCATGG 1 cut(s) 109
BspACI CCGC 2 cut(s) 181, 183
BspFNI CGCG 2 cut(s) 126, 183
BspLI GGNNCC 1 cut(s) 194
BspMAI CTGCAG 1 cut(s) 152
BspT107I GGYRCC 1 cut(s) 192
BssECI CCNNGG 2 cut(s) 109, 181
BssNI GRCGYC 1 cut(s) 193
BssSI CACGAG 1 cut(s) 155
BssT1I CCWWGG 1 cut(s) 109
Bst2BI CACGAG 1 cut(s) 155
Bst4CI ACNGT 1 cut(s) 137
BstACI GRCGYC 1 cut(s) 193
BstC8I GCNNGC 1 cut(s) 152
BstDSI CCRYGG 2 cut(s) 109, 181
BstFNI CGCG 2 cut(s) 126, 183
BstH2I RGCGCY 1 cut(s) 196
BstHHI GCGC 2 cut(s) 128, 195
BstMWI GCNNNNNNNGC 2 cut(s) 180, 192
BstNSI RCATGY 1 cut(s) 120
BstSFI CTRYAG 1 cut(s) 148
BstUI CGCG 2 cut(s) 126, 183
BtgI CCRYGG 2 cut(s) 109, 181
Cac8I GCNNGC 1 cut(s) 152
CfoI GCGC 2 cut(s) 128, 195
Cfr42I CCGCGG 1 cut(s) 184
CviAII CATG 3 cut(s) 101, 110, 117
CviJI RGCY 3 cut(s) 26, 77, 186
CviKI_1 RGCY 3 cut(s) 26, 77, 186
DinI GGCGCC 1 cut(s) 194
Eco130I CCWWGG 1 cut(s) 109
EcoT14I CCWWGG 1 cut(s) 109
EcoT22I ATGCAT 1 cut(s) 122
EgeI GGCGCC 1 cut(s) 194
EheI GGCGCC 1 cut(s) 194
ErhI CCWWGG 1 cut(s) 109
FaeI CATG 3 cut(s) 104, 113, 120
FaiI YATR 3 cut(s) 102, 111, 118
FaqI GGGAC 1 cut(s) 127
FatI CATG 3 cut(s) 100, 109, 116
Fnu4HI GCNGC 2 cut(s) 181, 184
Fsp4HI GCNGC 2 cut(s) 181, 184
FspBI CTAG 1 cut(s) 78
GlaI GCGC 2 cut(s) 127, 194
GluI GCNGC 2 cut(s) 181, 184
HaeII RGCGCY 1 cut(s) 196
HapII CCGG 1 cut(s) 190
HhaI GCGC 2 cut(s) 128, 195
Hin1I GRCGYC 1 cut(s) 193
Hin1II CATG 3 cut(s) 104, 113, 120
Hin6I GCGC 2 cut(s) 126, 193
HinP1I GCGC 2 cut(s) 126, 193
HinfI GANTC 3 cut(s) 89, 97, 105
HpaII CCGG 1 cut(s) 190
HpyCH4III ACNGT 1 cut(s) 137
HpyCH4V TGCA 4 cut(s) 38, 120, 150, 207
HpyF10VI GCNNNNNNNGC 2 cut(s) 180, 192
Hsp92I GRCGYC 1 cut(s) 193
Hsp92II CATG 3 cut(s) 104, 113, 120
HspAI GCGC 2 cut(s) 126, 193
KasI GGCGCC 1 cut(s) 192
KspI CCGCGG 1 cut(s) 184
LpnPI CCDG 3 cut(s) 136, 153, 203
LweI GCATC 2 cut(s) 167, 188
MaeI CTAG 1 cut(s) 78
MaeIII GTNAC 1 cut(s) 90
MhlI GDGCHC 1 cut(s) 142
MluCI AATT 3 cut(s) 5, 11, 17
Mly113I GGCGCC 1 cut(s) 193
MlyI GAGTC 2 cut(s) 98, 106
MnlI CCTC 2 cut(s) 67, 151
Mph1103I ATGCAT 1 cut(s) 122
MseI TTAA 1 cut(s) 14
MslI CAYNNNNRTG 2 cut(s) 56, 135
MspA1I CMGCKG 1 cut(s) 183
MspI CCGG 1 cut(s) 190
Mva1269I GAATGC 1 cut(s) 120
MvnI CGCG 2 cut(s) 126, 183
MwoI GCNNNNNNNGC 2 cut(s) 180, 192
NarI GGCGCC 1 cut(s) 193
NcoI CCATGG 1 cut(s) 109
NlaIII CATG 3 cut(s) 104, 113, 120
NlaIV GGNNCC 1 cut(s) 194
NmuCI GTSAC 1 cut(s) 90
NsiI ATGCAT 1 cut(s) 122
NspI RCATGY 1 cut(s) 120
OliI CACNNNNGTG 1 cut(s) 135
PctI GAATGC 1 cut(s) 120
PfeI GAWTC 1 cut(s) 105
PkrI GCNGC 2 cut(s) 182, 185
PleI GAGTC 2 cut(s) 97, 105
PluTI GGCGCC 1 cut(s) 196
PpsI GAGTC 2 cut(s) 97, 105
PspN4I GGNNCC 1 cut(s) 194
PstI CTGCAG 1 cut(s) 152
RseI CAYNNNNRTG 2 cut(s) 56, 135
SacII CCGCGG 1 cut(s) 184
SaqAI TTAA 1 cut(s) 14
SatI GCNGC 2 cut(s) 181, 184
SchI GAGTC 2 cut(s) 98, 106
SduI GDGCHC 1 cut(s) 142
SfaNI GCATC 2 cut(s) 167, 188
SfcI CTRYAG 1 cut(s) 148
SfoI GGCGCC 1 cut(s) 194
Sfr303I CCGCGG 1 cut(s) 184
SgrBI CCGCGG 1 cut(s) 184
SmiMI CAYNNNNRTG 2 cut(s) 56, 135
Sse9I AATT 3 cut(s) 5, 11, 17
SsiI CCGC 2 cut(s) 181, 183
SspDI GGCGCC 1 cut(s) 192
SspMI CTAG 1 cut(s) 78
StyI CCWWGG 1 cut(s) 109
TaaI ACNGT 1 cut(s) 137
TaqI TCGA 1 cut(s) 211
TasI AATT 3 cut(s) 5, 11, 17
TauI GCSGC 2 cut(s) 183, 186
TfiI GAWTC 1 cut(s) 105
Tru1I TTAA 1 cut(s) 14
Tru9I TTAA 1 cut(s) 14
TseFI GTSAC 1 cut(s) 90
Tsp45I GTSAC 1 cut(s) 90
TspDTI ATGAA 1 cut(s) 17
XceI RCATGY 1 cut(s) 120
XspI CTAG 1 cut(s) 78
Zsp2I ATGCAT 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.