RchiOBHm_Chr5g0024091

Knottins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001621
Physical Location & Seq
Forward (+)
18290605 .. 18290941
337 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30387

Sequence Viewer

Length: 225 bp
ATGGACAGGAAGTTCTTAGGGCTTGTTTTGTTCTTCGTCATCCTCCTGACTTCTCAAGAGACGGTGCTGCAGAGTGAAGCAAAGATCTGCGAGTACCCAAGCAGAACATTCAAAGGGCTGTGCGTCAAGGACAAAAACTGTGTCGTGAGATGTCGGAGTGAGGGTTACAATTACGGCAAATGCTCCCATGTCCGCCGACGATGTAGGTGCTTCAAGCCTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

74

Amino Acids

8.66

Weight (kDa)

9.43

Isoelectric Point (pI)

46.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 28 - 74 1e-13 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 193
AfaI GTAC 1 cut(s) 95
AgsI TTSAA 2 cut(s) 112, 214
AloI GAACNNNNNNTCC 1 cut(s) 28
Alw26I GTCTC 1 cut(s) 53
ApeKI GCWGC 1 cut(s) 67
ArsI GACNNNNNNTTYG 2 cut(s) 126, 158
BbvI GCAGC 1 cut(s) 54
BceAI ACGGC 1 cut(s) 190
BcgI CGANNNNNNTGC 2 cut(s) 189, 223
BcoDI GTCTC 1 cut(s) 53
BfmI CTRYAG 1 cut(s) 68
BglII AGATCT 1 cut(s) 84
BisI GCNGC 1 cut(s) 68
BlsI GCNGC 1 cut(s) 69
BpuEI CTTGAG 1 cut(s) 39
BsaXI ACNNNNNCTCC 2 cut(s) 148, 178
BseGI GGATG 1 cut(s) 39
BseXI GCAGC 1 cut(s) 54
BsmAI GTCTC 1 cut(s) 53
BsmBI CGTCTC 1 cut(s) 53
Bsp143I GATC 1 cut(s) 84
BspACI CCGC 1 cut(s) 193
BspMAI CTGCAG 1 cut(s) 72
BssMI GATC 1 cut(s) 84
Bst4CI ACNGT 2 cut(s) 64, 140
BstDEI CTNAG 1 cut(s) 16
BstF5I GGATG 1 cut(s) 39
BstKTI GATC 1 cut(s) 87
BstMAI GTCTC 1 cut(s) 53
BstMBI GATC 1 cut(s) 84
BstSFI CTRYAG 1 cut(s) 68
BstV1I GCAGC 1 cut(s) 54
BstX2I RGATCY 1 cut(s) 84
BstYI RGATCY 1 cut(s) 84
BtsCI GGATG 1 cut(s) 39
CseI GACGC 1 cut(s) 112
Csp6I GTAC 1 cut(s) 94
CviAII CATG 1 cut(s) 188
CviJI RGCY 3 cut(s) 22, 118, 217
CviKI_1 RGCY 3 cut(s) 22, 118, 217
CviQI GTAC 1 cut(s) 94
DdeI CTNAG 1 cut(s) 16
DpnI GATC 1 cut(s) 86
DpnII GATC 1 cut(s) 84
EciI GGCGGA 1 cut(s) 182
Esp3I CGTCTC 1 cut(s) 53
FaeI CATG 1 cut(s) 191
FaiI YATR 1 cut(s) 189
FatI CATG 1 cut(s) 187
Fnu4HI GCNGC 1 cut(s) 68
FokI GGATG 1 cut(s) 26
Fsp4HI GCNGC 1 cut(s) 68
GluI GCNGC 1 cut(s) 68
HgaI GACGC 1 cut(s) 112
Hin1II CATG 1 cut(s) 191
Hpy188I TCNGA 1 cut(s) 156
Hpy188III TCNNGA 3 cut(s) 46, 56, 145
Hpy99I CGWCG 1 cut(s) 201
HpyCH4III ACNGT 2 cut(s) 64, 140
HpyCH4V TGCA 1 cut(s) 70
HpyF3I CTNAG 1 cut(s) 16
Hsp92II CATG 1 cut(s) 191
Kzo9I GATC 1 cut(s) 84
LmnI GCTCC 1 cut(s) 188
LpnPI CCDG 1 cut(s) 59
Lsp1109I GCAGC 1 cut(s) 54
MaeIII GTNAC 1 cut(s) 164
MalI GATC 1 cut(s) 86
MboI GATC 1 cut(s) 84
MboII GAAGA 1 cut(s) 25
MflI RGATCY 1 cut(s) 84
MluCI AATT 1 cut(s) 169
MmeI TCCRAC 1 cut(s) 134
MnlI CCTC 2 cut(s) 53, 154
NdeII GATC 1 cut(s) 84
NlaIII CATG 1 cut(s) 191
PkrI GCNGC 1 cut(s) 69
PstI CTGCAG 1 cut(s) 72
PsuI RGATCY 1 cut(s) 84
RsaI GTAC 1 cut(s) 95
RsaNI GTAC 1 cut(s) 94
SatI GCNGC 1 cut(s) 68
Sau3AI GATC 1 cut(s) 84
SetI ASST 1 cut(s) 209
SfcI CTRYAG 1 cut(s) 68
SgeI CNNG 9 cut(s) 19, 35, 58, 68, 103, 111, 139, 157, 200
SmlI CTYRAG 1 cut(s) 54
SmoI CTYRAG 1 cut(s) 54
Sse9I AATT 1 cut(s) 169
SsiI CCGC 1 cut(s) 193
TaaI ACNGT 2 cut(s) 64, 140
TasI AATT 1 cut(s) 169
TseI GCWGC 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.