Rmu_sc0004593.1_g000016

Knottins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004593.1
OG0001621
Physical Location & Seq
Reverse (-)
103942 .. 104250
309 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004593.1_g000016.1.cds

Sequence Viewer

Length: 309 bp
atgcaggtgcaagttggagctgatgaatgtgagtatcgaagcgcacatttcagggggccctgctttggacgcaatcacgagtgctacatctgttgccaggaggaaggttttgatcggggctcatgcaaagggtttctcaaccgtcattgtatctgcactaagccttgtggatcacctggaggtcctcctaccaacgaacctcctccttctccagaagaagatgttcctcctcctccagaggaagatgttcctcctcctcccgaagaagatgttcctcctactcctgaagaagaagctcccactaattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

102

Amino Acids

11.15

Weight (kDa)

4.36

Isoelectric Point (pI)

89.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 178
AcuI CTGAAG 1 cut(s) 306
AfiI CCNNNNNNNGG 1 cut(s) 65
AjnI CCWGG 2 cut(s) 96, 175
AluBI AGCT 2 cut(s) 20, 296
AluI AGCT 2 cut(s) 20, 296
AlwI GGATC 1 cut(s) 178
AoxI GGCC 1 cut(s) 56
ApaI GGGCCC 1 cut(s) 60
Asp700I GAANNNNTTC 3 cut(s) 222, 246, 270
AspLEI GCGC 1 cut(s) 44
AspS9I GGNCC 3 cut(s) 56, 57, 182
AsuHPI GGTGA 1 cut(s) 165
AvaII GGWCC 1 cut(s) 182
BaeGI GKGCMC 1 cut(s) 60
BanII GRGCYC 2 cut(s) 60, 122
BauI CACGAG 1 cut(s) 77
BciT130I CCWGG 2 cut(s) 98, 177
Bme1390I CCNGG 2 cut(s) 98, 177
Bme18I GGWCC 1 cut(s) 182
BmgT120I GGNCC 3 cut(s) 56, 57, 182
BmiI GGNNCC 2 cut(s) 57, 58
BmrFI CCNGG 2 cut(s) 98, 177
BpmI CTGGAG 3 cut(s) 195, 198, 219
Bsc4I CCNNNNNNNGG 1 cut(s) 65
BseBI CCWGG 2 cut(s) 98, 177
BseLI CCNNNNNNNGG 1 cut(s) 65
BseRI GAGGAG 5 cut(s) 192, 219, 222, 243, 246
BseSI GKGCMC 1 cut(s) 60
BsgI GTGCAG 1 cut(s) 139
BshFI GGCC 1 cut(s) 58
BslI CCNNNNNNNGG 1 cut(s) 65
BsnI GGCC 1 cut(s) 58
Bsp120I GGGCCC 1 cut(s) 56
Bsp1286I GDGCHC 2 cut(s) 60, 122
Bsp143I GATC 2 cut(s) 112, 170
BspANI GGCC 1 cut(s) 58
BspLI GGNNCC 2 cut(s) 57, 58
BspPI GGATC 1 cut(s) 178
BssMI GATC 2 cut(s) 112, 170
BssSI CACGAG 1 cut(s) 77
Bst2BI CACGAG 1 cut(s) 77
Bst2UI CCWGG 2 cut(s) 98, 177
Bst4CI ACNGT 1 cut(s) 143
BstDEI CTNAG 1 cut(s) 159
BstHHI GCGC 1 cut(s) 44
BstKTI GATC 2 cut(s) 115, 173
BstMBI GATC 2 cut(s) 112, 170
BstMWI GCNNNNNNNGC 1 cut(s) 69
BstNI CCWGG 2 cut(s) 98, 177
BstSCI CCNGG 2 cut(s) 96, 175
BstSLI GKGCMC 1 cut(s) 60
BsuRI GGCC 1 cut(s) 58
CfoI GCGC 1 cut(s) 44
Cfr13I GGNCC 3 cut(s) 56, 57, 182
CseI GACGC 1 cut(s) 78
CviAII CATG 1 cut(s) 123
CviJI RGCY 5 cut(s) 20, 58, 120, 163, 296
CviKI_1 RGCY 5 cut(s) 20, 58, 120, 163, 296
DdeI CTNAG 1 cut(s) 159
DpnI GATC 2 cut(s) 114, 172
DpnII GATC 2 cut(s) 112, 170
Eco24I GRGCYC 2 cut(s) 60, 122
Eco47I GGWCC 1 cut(s) 182
Eco57I CTGAAG 1 cut(s) 306
EcoO109I RGGNCCY 3 cut(s) 56, 57, 182
EcoRII CCWGG 2 cut(s) 96, 175
EcoT38I GRGCYC 2 cut(s) 60, 122
FaeI CATG 1 cut(s) 126
FaiI YATR 1 cut(s) 124
FatI CATG 1 cut(s) 122
FriOI GRGCYC 2 cut(s) 60, 122
GlaI GCGC 1 cut(s) 43
GsuI CTGGAG 3 cut(s) 195, 198, 219
HaeIII GGCC 1 cut(s) 58
HgaI GACGC 1 cut(s) 78
HhaI GCGC 1 cut(s) 44
Hin1II CATG 1 cut(s) 126
Hin6I GCGC 1 cut(s) 42
HinP1I GCGC 1 cut(s) 42
HphI GGTGA 1 cut(s) 165
Hpy188III TCNNGA 5 cut(s) 77, 212, 236, 260, 284
HpyAV CCTTC 2 cut(s) 98, 216
HpyCH4III ACNGT 1 cut(s) 143
HpyCH4V TGCA 4 cut(s) 4, 10, 126, 156
HpyF10VI GCNNNNNNNGC 1 cut(s) 69
HpyF3I CTNAG 1 cut(s) 159
Hsp92II CATG 1 cut(s) 126
HspAI GCGC 1 cut(s) 42
Kzo9I GATC 2 cut(s) 112, 170
LmnI GCTCC 2 cut(s) 17, 301
LpnPI CCDG 9 cut(s) 37, 73, 83, 110, 162, 189, 225, 249, 297
MalI GATC 2 cut(s) 114, 172
MboI GATC 2 cut(s) 112, 170
MboII GAAGA 7 cut(s) 227, 230, 254, 275, 278, 299, 302
MhlI GDGCHC 2 cut(s) 60, 122
MluCI AATT 1 cut(s) 304
MroXI GAANNNNTTC 3 cut(s) 222, 246, 270
MspR9I CCNGG 2 cut(s) 98, 177
MvaI CCWGG 2 cut(s) 98, 177
MwoI GCNNNNNNNGC 1 cut(s) 69
NdeII GATC 2 cut(s) 112, 170
NlaIII CATG 1 cut(s) 126
NlaIV GGNNCC 2 cut(s) 57, 58
PdmI GAANNNNTTC 3 cut(s) 222, 246, 270
PpuMI RGGWCCY 1 cut(s) 182
Psp5II RGGWCCY 1 cut(s) 182
Psp6I CCWGG 2 cut(s) 96, 175
PspGI CCWGG 2 cut(s) 96, 175
PspN4I GGNNCC 2 cut(s) 57, 58
PspOMI GGGCCC 1 cut(s) 56
PspPI GGNCC 3 cut(s) 56, 57, 182
PspPPI RGGWCCY 1 cut(s) 182
Sau3AI GATC 2 cut(s) 112, 170
Sau96I GGNCC 3 cut(s) 56, 57, 182
ScrFI CCNGG 2 cut(s) 98, 177
SduI GDGCHC 2 cut(s) 60, 122
SetI ASST 7 cut(s) 9, 22, 109, 178, 184, 202, 298
SinI GGWCC 1 cut(s) 182
Sse9I AATT 1 cut(s) 304
StyD4I CCNGG 2 cut(s) 96, 175
TaaI ACNGT 1 cut(s) 143
TaqI TCGA 1 cut(s) 37
TasI AATT 1 cut(s) 304
TspDTI ATGAA 1 cut(s) 39
VpaK11BI GGWCC 1 cut(s) 182
XmnI GAANNNNTTC 3 cut(s) 222, 246, 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.