Rroxscaffold_1G00055150

Knottins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001621
Physical Location & Seq
Reverse (-)
76605934 .. 76625474
19541 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00055150.1

Sequence Viewer

Length: 228 bp
ATGGACAGGAAGTTCTTAGGGCTTGTTCTGTTCTTCATCGTCCTCCTGACTTCTCAAGAGATGGTGATTCGCAGCGAAGCGCAGATATGCGAAGTCCCGAGCAAAACATTCAAGGGGTTGTGCATCAAGGACAGGAACTGTGTCGTGAGATGTCACAGTGAGGGTTACGGTTACGGCAAATGCTCCCTTGTCCGACGACAGTGCATGTGCTTGAAGATTTGTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

75

Amino Acids

8.61

Weight (kDa)

8.88

Isoelectric Point (pI)

40.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 28 - 74 1.3e-14 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AgsI TTSAA 2 cut(s) 112, 214
AloI GAACNNNNNNTCC 1 cut(s) 28
AlwNI CAGNNNCTG 1 cut(s) 138
Ama87I CYCGRG 1 cut(s) 97
ApeKI GCWGC 1 cut(s) 72
AspLEI GCGC 1 cut(s) 82
AsuHPI GGTGA 1 cut(s) 76
AvaI CYCGRG 1 cut(s) 97
BbvI GCAGC 1 cut(s) 84
BccI CCATC 1 cut(s) 55
BceAI ACGGC 1 cut(s) 190
BcgI CGANNNNNNTGC 2 cut(s) 183, 217
BisI GCNGC 1 cut(s) 73
BlsI GCNGC 1 cut(s) 74
BmeT110I CYCGRG 1 cut(s) 97
BmsI GCATC 1 cut(s) 132
BpuEI CTTGAG 1 cut(s) 39
BseXI GCAGC 1 cut(s) 84
BsiHKCI CYCGRG 1 cut(s) 97
BslFI GGGAC 1 cut(s) 80
BsmFI GGGAC 1 cut(s) 80
BsoBI CYCGRG 1 cut(s) 97
Bst4CI ACNGT 4 cut(s) 140, 158, 170, 201
BstDEI CTNAG 1 cut(s) 16
BstHHI GCGC 1 cut(s) 82
BstNSI RCATGY 1 cut(s) 208
BstV1I GCAGC 1 cut(s) 84
BtsIMutI CAGTG 2 cut(s) 163, 206
CaiI CAGNNNCTG 1 cut(s) 138
CfoI GCGC 1 cut(s) 82
CviAII CATG 1 cut(s) 205
CviJI RGCY 1 cut(s) 22
CviKI_1 RGCY 1 cut(s) 22
DdeI CTNAG 1 cut(s) 16
Eco88I CYCGRG 1 cut(s) 97
FaeI CATG 1 cut(s) 208
FaiI YATR 2 cut(s) 88, 206
FaqI GGGAC 1 cut(s) 80
FatI CATG 1 cut(s) 204
Fnu4HI GCNGC 1 cut(s) 73
Fsp4HI GCNGC 1 cut(s) 73
GlaI GCGC 1 cut(s) 81
GluI GCNGC 1 cut(s) 73
HhaI GCGC 1 cut(s) 82
Hin1II CATG 1 cut(s) 208
Hin6I GCGC 1 cut(s) 80
HinP1I GCGC 1 cut(s) 80
HinfI GANTC 1 cut(s) 67
HphI GGTGA 1 cut(s) 76
Hpy188I TCNGA 1 cut(s) 194
Hpy188III TCNNGA 4 cut(s) 46, 56, 97, 145
Hpy99I CGWCG 1 cut(s) 198
HpyCH4III ACNGT 4 cut(s) 140, 158, 170, 201
HpyCH4V TGCA 2 cut(s) 123, 204
HpyF3I CTNAG 1 cut(s) 16
Hsp92II CATG 1 cut(s) 208
HspAI GCGC 1 cut(s) 80
LmnI GCTCC 1 cut(s) 188
LpnPI CCDG 2 cut(s) 59, 118
Lsp1109I GCAGC 1 cut(s) 84
LweI GCATC 1 cut(s) 132
MaeIII GTNAC 4 cut(s) 152, 164, 170, 221
MboII GAAGA 2 cut(s) 25, 226
MmeI TCCRAC 1 cut(s) 217
MnlI CCTC 2 cut(s) 53, 154
NlaIII CATG 1 cut(s) 208
NmuCI GTSAC 2 cut(s) 152, 221
NspI RCATGY 1 cut(s) 208
PfeI GAWTC 1 cut(s) 67
PkrI GCNGC 1 cut(s) 74
PstNI CAGNNNCTG 1 cut(s) 138
SatI GCNGC 1 cut(s) 73
SfaNI GCATC 1 cut(s) 132
SmlI CTYRAG 1 cut(s) 54
SmoI CTYRAG 1 cut(s) 54
TaaI ACNGT 4 cut(s) 140, 158, 170, 201
TfiI GAWTC 1 cut(s) 67
TscAI CASTG 2 cut(s) 163, 206
TseFI GTSAC 2 cut(s) 152, 221
TseI GCWGC 1 cut(s) 72
Tsp45I GTSAC 2 cut(s) 152, 221
TspDTI ATGAA 1 cut(s) 25
TspRI CASTG 2 cut(s) 163, 206
XceI RCATGY 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.