Rh5DG170600

Knottins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001621
Physical Location & Seq
Forward (+)
18298300 .. 18309462
11163 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG170600.1

Sequence Viewer

Length: 246 bp
ATGGAGAGGAAGTACTTTGGGCTTGTTTTGTTCTTCATCATCCTCCTAACTTCTCAAGAGATGGTGATTCAGAGTGAAGCAAGAGTTTGCGAAGTGCCAAGCAAGACATTCAGCGGGCTGTGCTTCAGTGACACCCACTGCATCGTGAGATGCCACAGTGAGGGTTACGAAGAGATGGTGGTGCACAGTGAAGCAAGGATCTGCGAAGTTCCAAGCAAGACATTGAGCGGGCTTCCACGAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

81

Amino Acids

9.23

Weight (kDa)

5.57

Isoelectric Point (pI)

45.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 28 - 56 1e-06 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 228
AciI CCGC 2 cut(s) 114, 228
AclWI GGATC 1 cut(s) 206
AcuI CTGAAG 1 cut(s) 109
AfaI GTAC 1 cut(s) 14
AfiI CCNNNNNNNGG 1 cut(s) 160
Alw21I GWGCWC 1 cut(s) 186
Alw44I GTGCAC 1 cut(s) 182
AlwI GGATC 1 cut(s) 206
ApaLI GTGCAC 1 cut(s) 182
AsuHPI GGTGA 1 cut(s) 76
BaeGI GKGCMC 1 cut(s) 186
BauI CACGAG 1 cut(s) 237
Bbv12I GWGCWC 1 cut(s) 186
BccI CCATC 2 cut(s) 55, 169
BmcAI AGTACT 1 cut(s) 14
BmsI GCATC 2 cut(s) 140, 150
BpuEI CTTGAG 1 cut(s) 39
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 1 cut(s) 160
BseGI GGATG 1 cut(s) 39
BseLI CCNNNNNNNGG 1 cut(s) 160
BseSI GKGCMC 1 cut(s) 186
BsiHKAI GWGCWC 1 cut(s) 186
BslI CCNNNNNNNGG 1 cut(s) 160
Bsp1286I GDGCHC 1 cut(s) 186
Bsp143I GATC 1 cut(s) 198
BspACI CCGC 2 cut(s) 114, 228
BspPI GGATC 1 cut(s) 206
BsrBI CCGCTC 1 cut(s) 228
BssMI GATC 1 cut(s) 198
BssSI CACGAG 1 cut(s) 237
Bst2BI CACGAG 1 cut(s) 237
Bst4CI ACNGT 2 cut(s) 158, 188
Bst6I CTCTTC 1 cut(s) 165
BstC8I GCNNGC 2 cut(s) 116, 230
BstF5I GGATG 1 cut(s) 39
BstKTI GATC 1 cut(s) 201
BstMBI GATC 1 cut(s) 198
BstMWI GCNNNNNNNGC 1 cut(s) 120
BstSLI GKGCMC 1 cut(s) 186
BstX2I RGATCY 1 cut(s) 198
BstYI RGATCY 1 cut(s) 198
BtsCI GGATG 1 cut(s) 39
BtsI GCAGTG 1 cut(s) 136
BtsIMutI CAGTG 4 cut(s) 133, 136, 163, 193
Cac8I GCNNGC 2 cut(s) 116, 230
Csp6I GTAC 1 cut(s) 13
CviJI RGCY 3 cut(s) 22, 118, 232
CviKI_1 RGCY 3 cut(s) 22, 118, 232
CviQI GTAC 1 cut(s) 13
DpnI GATC 1 cut(s) 200
DpnII GATC 1 cut(s) 198
Eam1104I CTCTTC 1 cut(s) 165
EarI CTCTTC 1 cut(s) 165
Eco57I CTGAAG 1 cut(s) 109
FauI CCCGC 2 cut(s) 107, 221
FokI GGATG 1 cut(s) 26
HinfI GANTC 1 cut(s) 67
HphI GGTGA 1 cut(s) 76
Hpy166II GTNNAC 1 cut(s) 184
Hpy188I TCNGA 1 cut(s) 72
Hpy188III TCNNGA 2 cut(s) 56, 145
Hpy8I GTNNAC 1 cut(s) 184
HpyCH4III ACNGT 2 cut(s) 158, 188
HpyCH4V TGCA 2 cut(s) 141, 184
HpyF10VI GCNNNNNNNGC 1 cut(s) 120
Kzo9I GATC 1 cut(s) 198
LweI GCATC 2 cut(s) 140, 150
MaeIII GTNAC 2 cut(s) 128, 164
MalI GATC 1 cut(s) 200
MbiI CCGCTC 1 cut(s) 228
MboI GATC 1 cut(s) 198
MboII GAAGA 2 cut(s) 25, 182
MflI RGATCY 1 cut(s) 198
MhlI GDGCHC 1 cut(s) 186
MnlI CCTC 2 cut(s) 53, 154
MspA1I CMGCKG 1 cut(s) 114
MwoI GCNNNNNNNGC 1 cut(s) 120
NdeII GATC 1 cut(s) 198
NmuCI GTSAC 1 cut(s) 128
PfeI GAWTC 1 cut(s) 67
PsuI RGATCY 1 cut(s) 198
RsaI GTAC 1 cut(s) 14
RsaNI GTAC 1 cut(s) 13
Sau3AI GATC 1 cut(s) 198
ScaI AGTACT 1 cut(s) 14
SduI GDGCHC 1 cut(s) 186
SfaNI GCATC 2 cut(s) 140, 150
SmlI CTYRAG 1 cut(s) 54
SmoI CTYRAG 1 cut(s) 54
SsiI CCGC 2 cut(s) 114, 228
TaaI ACNGT 2 cut(s) 158, 188
TatI WGTACW 1 cut(s) 12
TfiI GAWTC 1 cut(s) 67
TscAI CASTG 4 cut(s) 133, 143, 163, 193
TseFI GTSAC 1 cut(s) 128
Tsp45I GTSAC 1 cut(s) 128
TspDTI ATGAA 1 cut(s) 25
TspRI CASTG 4 cut(s) 133, 143, 163, 193
VneI GTGCAC 1 cut(s) 182
ZrmI AGTACT 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.