RchiOBHm_Chr5g0024081

Knottins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001621
Physical Location & Seq
Forward (+)
18279260 .. 18279571
312 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30386

Sequence Viewer

Length: 228 bp
ATGGATAGGAAGTTCTTGGGGCTTTTACTGTTCTTCATCATCCTCCTGAATTCTCAAGAGATGGTGATTCGCAGCGAAGCGAAGATATGCGAAGTCCCGAGCAAAACATTCAAGGGGTTGTGCGTCAAGGACAGGAACTGTGTCGTGAGATGTCACAGTGAGGGTTACGGTAACGGCAAATGCTCCCATGTCCGACGACAGTGCATGTGCTTGAAGATTTGTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

75

Amino Acids

8.61

Weight (kDa)

9.04

Isoelectric Point (pI)

42.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 28 - 74 8.7e-15 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 49
AgsI TTSAA 2 cut(s) 112, 214
AloI GAACNNNNNNTCC 1 cut(s) 28
AlwNI CAGNNNCTG 1 cut(s) 138
Ama87I CYCGRG 1 cut(s) 97
ApeKI GCWGC 1 cut(s) 72
ApoI RAATTY 1 cut(s) 49
AsuHPI GGTGA 1 cut(s) 76
AvaI CYCGRG 1 cut(s) 97
BbvI GCAGC 1 cut(s) 84
BccI CCATC 1 cut(s) 55
BceAI ACGGC 1 cut(s) 190
BcgI CGANNNNNNTGC 2 cut(s) 183, 217
BisI GCNGC 1 cut(s) 73
BlsI GCNGC 1 cut(s) 74
BmeT110I CYCGRG 1 cut(s) 97
BpuEI CTTGAG 1 cut(s) 39
BseGI GGATG 1 cut(s) 39
BseXI GCAGC 1 cut(s) 84
BsiHKCI CYCGRG 1 cut(s) 97
BslFI GGGAC 1 cut(s) 80
BsmFI GGGAC 1 cut(s) 80
BsoBI CYCGRG 1 cut(s) 97
Bst4CI ACNGT 5 cut(s) 30, 140, 158, 170, 201
BstF5I GGATG 1 cut(s) 39
BstNSI RCATGY 1 cut(s) 208
BstV1I GCAGC 1 cut(s) 84
BtsCI GGATG 1 cut(s) 39
BtsIMutI CAGTG 2 cut(s) 163, 206
CaiI CAGNNNCTG 1 cut(s) 138
CseI GACGC 1 cut(s) 112
CviAII CATG 2 cut(s) 188, 205
CviJI RGCY 1 cut(s) 22
CviKI_1 RGCY 1 cut(s) 22
Eco88I CYCGRG 1 cut(s) 97
EcoRI GAATTC 1 cut(s) 49
FaeI CATG 2 cut(s) 191, 208
FaiI YATR 3 cut(s) 88, 189, 206
FaqI GGGAC 1 cut(s) 80
FatI CATG 2 cut(s) 187, 204
Fnu4HI GCNGC 1 cut(s) 73
FokI GGATG 1 cut(s) 26
Fsp4HI GCNGC 1 cut(s) 73
GluI GCNGC 1 cut(s) 73
HgaI GACGC 1 cut(s) 112
Hin1II CATG 2 cut(s) 191, 208
HinfI GANTC 1 cut(s) 67
HphI GGTGA 1 cut(s) 76
Hpy188I TCNGA 1 cut(s) 194
Hpy188III TCNNGA 4 cut(s) 46, 56, 97, 145
Hpy99I CGWCG 1 cut(s) 198
HpyCH4III ACNGT 5 cut(s) 30, 140, 158, 170, 201
HpyCH4V TGCA 1 cut(s) 204
Hsp92II CATG 2 cut(s) 191, 208
LmnI GCTCC 1 cut(s) 188
LpnPI CCDG 2 cut(s) 59, 118
Lsp1109I GCAGC 1 cut(s) 84
MaeIII GTNAC 4 cut(s) 152, 164, 170, 221
MboII GAAGA 3 cut(s) 25, 94, 226
MluCI AATT 1 cut(s) 49
MmeI TCCRAC 1 cut(s) 217
MnlI CCTC 2 cut(s) 53, 154
NlaIII CATG 2 cut(s) 191, 208
NmuCI GTSAC 2 cut(s) 152, 221
NspI RCATGY 1 cut(s) 208
PfeI GAWTC 1 cut(s) 67
PkrI GCNGC 1 cut(s) 74
PstNI CAGNNNCTG 1 cut(s) 138
SatI GCNGC 1 cut(s) 73
SmlI CTYRAG 1 cut(s) 54
SmoI CTYRAG 1 cut(s) 54
Sse9I AATT 1 cut(s) 49
TaaI ACNGT 5 cut(s) 30, 140, 158, 170, 201
TasI AATT 1 cut(s) 49
TfiI GAWTC 1 cut(s) 67
TscAI CASTG 2 cut(s) 163, 206
TseFI GTSAC 2 cut(s) 152, 221
TseI GCWGC 1 cut(s) 72
Tsp45I GTSAC 2 cut(s) 152, 221
TspDTI ATGAA 1 cut(s) 25
TspRI CASTG 2 cut(s) 163, 206
XapI RAATTY 1 cut(s) 49
XceI RCATGY 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.