Rh7DG388400

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0001621
Physical Location & Seq
Forward (+)
53144612 .. 53144945
334 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG388400.1

Sequence Viewer

Length: 225 bp
ATGGGGAGCAAATGTCTGGTTGTTTTCTTTTTGCTCTTCATTGTTTTCGCTTCACAAGAGATGGTAGTGCCGAGTGAGGCTAGGGTCTGCGAGTCACAGAGTCATGGATTCCATGGGACATGCATTCGCGCCCACAACTGTGCTCTCGTCTGCAGGCACGAGGGTTTCTCCGGTGGAAGATGCCGCGGCTTCCGGCGCCGATGCTTTTGCACTCGCAGATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

74

Amino Acids

8.39

Weight (kDa)

9.27

Isoelectric Point (pI)

63.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 28 - 74 2e-17 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 195
AccII CGCG 2 cut(s) 129, 186
AciI CCGC 2 cut(s) 184, 186
AcyI GRCGYC 1 cut(s) 196
AleI CACNNNNGTG 1 cut(s) 138
Alw21I GWGCWC 1 cut(s) 145
AspLEI GCGC 2 cut(s) 131, 198
BanI GGYRCC 1 cut(s) 195
BauI CACGAG 1 cut(s) 158
Bbv12I GWGCWC 1 cut(s) 145
BccI CCATC 1 cut(s) 55
BcgI CGANNNNNNTGC 2 cut(s) 189, 223
BfaI CTAG 1 cut(s) 81
BfmI CTRYAG 1 cut(s) 151
BfoI RGCGCY 1 cut(s) 199
BisI GCNGC 2 cut(s) 184, 187
BlsI GCNGC 2 cut(s) 185, 188
BmiI GGNNCC 1 cut(s) 197
BmsI GCATC 2 cut(s) 170, 191
BplI GAGNNNNNCTC 2 cut(s) 152, 184
BsaHI GRCGYC 1 cut(s) 196
BsaJI CCNNGG 2 cut(s) 112, 184
BsaWI WCCGGW 1 cut(s) 170
BsaXI ACNNNNNCTCC 1 cut(s) 28
BseDI CCNNGG 2 cut(s) 112, 184
Bsh1236I CGCG 2 cut(s) 129, 186
BshNI GGYRCC 1 cut(s) 195
BsiHKAI GWGCWC 1 cut(s) 145
BsiSI CCGG 2 cut(s) 171, 193
BslFI GGGAC 1 cut(s) 130
BsmFI GGGAC 1 cut(s) 130
BsmI GAATGC 1 cut(s) 123
Bsp1286I GDGCHC 1 cut(s) 145
Bsp19I CCATGG 1 cut(s) 112
BspACI CCGC 2 cut(s) 184, 186
BspFNI CGCG 2 cut(s) 129, 186
BspLI GGNNCC 1 cut(s) 197
BspMAI CTGCAG 1 cut(s) 155
BspQI GCTCTTC 1 cut(s) 41
BspT107I GGYRCC 1 cut(s) 195
BssECI CCNNGG 2 cut(s) 112, 184
BssNI GRCGYC 1 cut(s) 196
BssSI CACGAG 1 cut(s) 158
BssT1I CCWWGG 1 cut(s) 112
Bst2BI CACGAG 1 cut(s) 158
Bst4CI ACNGT 1 cut(s) 140
Bst6I CTCTTC 1 cut(s) 41
BstACI GRCGYC 1 cut(s) 196
BstC8I GCNNGC 1 cut(s) 155
BstDSI CCRYGG 2 cut(s) 112, 184
BstFNI CGCG 2 cut(s) 129, 186
BstH2I RGCGCY 1 cut(s) 199
BstHHI GCGC 2 cut(s) 131, 198
BstMWI GCNNNNNNNGC 1 cut(s) 195
BstNSI RCATGY 1 cut(s) 123
BstSFI CTRYAG 1 cut(s) 151
BstUI CGCG 2 cut(s) 129, 186
BtgI CCRYGG 2 cut(s) 112, 184
Cac8I GCNNGC 1 cut(s) 155
CfoI GCGC 2 cut(s) 131, 198
Cfr42I CCGCGG 1 cut(s) 187
CviAII CATG 3 cut(s) 104, 113, 120
CviJI RGCY 2 cut(s) 80, 189
CviKI_1 RGCY 2 cut(s) 80, 189
DinI GGCGCC 1 cut(s) 197
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Eco130I CCWWGG 1 cut(s) 112
EcoT14I CCWWGG 1 cut(s) 112
EcoT22I ATGCAT 1 cut(s) 125
EgeI GGCGCC 1 cut(s) 197
EheI GGCGCC 1 cut(s) 197
ErhI CCWWGG 1 cut(s) 112
FaeI CATG 3 cut(s) 107, 116, 123
FaiI YATR 3 cut(s) 105, 114, 121
FaqI GGGAC 1 cut(s) 130
FatI CATG 3 cut(s) 103, 112, 119
Fnu4HI GCNGC 2 cut(s) 184, 187
Fsp4HI GCNGC 2 cut(s) 184, 187
FspBI CTAG 1 cut(s) 81
GlaI GCGC 2 cut(s) 130, 197
GluI GCNGC 2 cut(s) 184, 187
HaeII RGCGCY 1 cut(s) 199
HapII CCGG 2 cut(s) 171, 193
HhaI GCGC 2 cut(s) 131, 198
Hin1I GRCGYC 1 cut(s) 196
Hin1II CATG 3 cut(s) 107, 116, 123
Hin6I GCGC 2 cut(s) 129, 196
HinP1I GCGC 2 cut(s) 129, 196
HinfI GANTC 3 cut(s) 92, 100, 108
HpaII CCGG 2 cut(s) 171, 193
HpyCH4III ACNGT 1 cut(s) 140
HpyCH4V TGCA 3 cut(s) 123, 153, 210
HpyF10VI GCNNNNNNNGC 1 cut(s) 195
Hsp92I GRCGYC 1 cut(s) 196
Hsp92II CATG 3 cut(s) 107, 116, 123
HspAI GCGC 2 cut(s) 129, 196
KasI GGCGCC 1 cut(s) 195
KspI CCGCGG 1 cut(s) 187
LguI GCTCTTC 1 cut(s) 41
LmnI GCTCC 1 cut(s) 6
LpnPI CCDG 4 cut(s) 2, 139, 184, 206
LweI GCATC 2 cut(s) 170, 191
MaeI CTAG 1 cut(s) 81
MaeIII GTNAC 1 cut(s) 93
MboII GAAGA 2 cut(s) 28, 189
MhlI GDGCHC 1 cut(s) 145
Mly113I GGCGCC 1 cut(s) 196
MlyI GAGTC 2 cut(s) 101, 109
MnlI CCTC 2 cut(s) 70, 154
Mph1103I ATGCAT 1 cut(s) 125
MslI CAYNNNNRTG 1 cut(s) 138
MspA1I CMGCKG 1 cut(s) 186
MspI CCGG 2 cut(s) 171, 193
Mva1269I GAATGC 1 cut(s) 123
MvnI CGCG 2 cut(s) 129, 186
MwoI GCNNNNNNNGC 1 cut(s) 195
NarI GGCGCC 1 cut(s) 196
NcoI CCATGG 1 cut(s) 112
NlaIII CATG 3 cut(s) 107, 116, 123
NlaIV GGNNCC 1 cut(s) 197
NmeAIII GCCGAG 1 cut(s) 96
NmuCI GTSAC 1 cut(s) 93
NsiI ATGCAT 1 cut(s) 125
NspI RCATGY 1 cut(s) 123
OliI CACNNNNGTG 1 cut(s) 138
PciSI GCTCTTC 1 cut(s) 41
PctI GAATGC 1 cut(s) 123
PfeI GAWTC 1 cut(s) 108
PkrI GCNGC 2 cut(s) 185, 188
PleI GAGTC 2 cut(s) 100, 108
PluTI GGCGCC 1 cut(s) 199
PpsI GAGTC 2 cut(s) 100, 108
PspN4I GGNNCC 1 cut(s) 197
PstI CTGCAG 1 cut(s) 155
RseI CAYNNNNRTG 1 cut(s) 138
SacII CCGCGG 1 cut(s) 187
SapI GCTCTTC 1 cut(s) 41
SatI GCNGC 2 cut(s) 184, 187
SchI GAGTC 2 cut(s) 101, 109
SduI GDGCHC 1 cut(s) 145
SfaNI GCATC 2 cut(s) 170, 191
SfcI CTRYAG 1 cut(s) 151
SfoI GGCGCC 1 cut(s) 197
Sfr303I CCGCGG 1 cut(s) 187
SgrBI CCGCGG 1 cut(s) 187
SmiMI CAYNNNNRTG 1 cut(s) 138
SsiI CCGC 2 cut(s) 184, 186
SspDI GGCGCC 1 cut(s) 195
SspMI CTAG 1 cut(s) 81
StyI CCWWGG 1 cut(s) 112
TaaI ACNGT 1 cut(s) 140
TauI GCSGC 2 cut(s) 186, 189
TfiI GAWTC 1 cut(s) 108
TseFI GTSAC 1 cut(s) 93
Tsp45I GTSAC 1 cut(s) 93
TspDTI ATGAA 1 cut(s) 28
XceI RCATGY 1 cut(s) 123
XspI CTAG 1 cut(s) 81
Zsp2I ATGCAT 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.