FvH4_3g34390

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002864
Physical Location & Seq
Forward (+)
29848832 .. 29849912
1081 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g34390.t1

Sequence Viewer

Length: 498 bp
ATGTGTCCTAATCCAGATTGGCACCCGACCTTTCAGTGCCCCAACCTCACTCCTGAGCTCAAGGAATGGCTAAAAGAAGCAAGACTCAAAGGTGGAACATTTAGGGCTTTCCACCCTGATGACCGGTATTGTCACGTTTGTTTGTCGAAAGACCACTGGTCTTCAGCATGCCCGTACCAGATTGAACTTCCAGTAGGTGCAACAATTGGCCCTTTTGCTGAAATACTCTGTCTGTGTTGTGGTGAGGATAGACCTCACCCTGGTGTGCCTGGTGTCGACTGGAAAGCAAGAGTTGTTCTCAAGGATTCTTGTATGTTAGGTAGCCCTGAAGCTTTAAGAAGGGCAGCTGCCGCCCGCGGCATCAAGGCAAATACATCTGCAGCAGCCAGTGGCACTGTCCTGAAGGCAAGTGCTTCAAAAGCCACAACTGCTGGCACTGCCACCTCCACCTCCACATCTGATGAGTACTTGGTTTCTTCAACCAGCCTTTCTGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

166

Amino Acids

17.59

Weight (kDa)

7.56

Isoelectric Point (pI)

40.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 21
AccI GTMKAC 1 cut(s) 276
AccII CGCG 1 cut(s) 357
AciI CCGC 3 cut(s) 351, 355, 357
AcuI CTGAAG 3 cut(s) 147, 348, 422
AfaI GTAC 2 cut(s) 176, 467
AfiI CCNNNNNNNGG 1 cut(s) 260
AgeI ACCGGT 1 cut(s) 123
AgsI TTSAA 3 cut(s) 185, 417, 480
AhdI GACNNNNNGTC 1 cut(s) 157
AjnI CCWGG 2 cut(s) 259, 268
AleI CACNNNNGTG 1 cut(s) 261
AluBI AGCT 3 cut(s) 58, 332, 347
AluI AGCT 3 cut(s) 58, 332, 347
Alw21I GWGCWC 1 cut(s) 60
AoxI GGCC 1 cut(s) 208
ApeKI GCWGC 4 cut(s) 344, 347, 380, 383
AsiGI ACCGGT 1 cut(s) 123
AspS9I GGNCC 1 cut(s) 209
AsuHPI GGTGA 2 cut(s) 248, 254
BaeGI GKGCMC 1 cut(s) 41
BanI GGYRCC 1 cut(s) 21
BanII GRGCYC 1 cut(s) 60
BbsI GAAGAC 1 cut(s) 153
Bbv12I GWGCWC 1 cut(s) 60
BbvI GCAGC 4 cut(s) 334, 356, 392, 395
BciT130I CCWGG 2 cut(s) 261, 270
BfmI CTRYAG 1 cut(s) 378
BisI GCNGC 6 cut(s) 345, 348, 351, 358, 381, 384
BlsI GCNGC 6 cut(s) 346, 349, 352, 359, 382, 385
BmcAI AGTACT 1 cut(s) 467
Bme1390I CCNGG 2 cut(s) 261, 270
BmeRI GACNNNNNGTC 1 cut(s) 157
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 1 cut(s) 23
BmrFI CCNGG 2 cut(s) 261, 270
BmsI GCATC 1 cut(s) 369
BpiI GAAGAC 1 cut(s) 153
BplI GAGNNNNNCTC 2 cut(s) 282, 314
Bpu10I CCTNAGC 1 cut(s) 54
BpuEI CTTGAG 2 cut(s) 44, 284
BsaJI CCNNGG 2 cut(s) 259, 355
BsaWI WCCGGW 1 cut(s) 123
Bsc4I CCNNNNNNNGG 1 cut(s) 260
Bse118I RCCGGY 1 cut(s) 123
Bse1I ACTGG 4 cut(s) 161, 191, 284, 387
BseBI CCWGG 2 cut(s) 261, 270
BseDI CCNNGG 2 cut(s) 259, 355
BseLI CCNNNNNNNGG 1 cut(s) 260
BseMII CTCAG 1 cut(s) 45
BseNI ACTGG 4 cut(s) 161, 191, 284, 387
BseSI GKGCMC 1 cut(s) 41
BseXI GCAGC 4 cut(s) 334, 356, 392, 395
Bsh1236I CGCG 1 cut(s) 357
BshFI GGCC 1 cut(s) 210
BshNI GGYRCC 1 cut(s) 21
BshTI ACCGGT 1 cut(s) 123
BsiHKAI GWGCWC 1 cut(s) 60
BsiSI CCGG 1 cut(s) 124
BslI CCNNNNNNNGG 1 cut(s) 260
BsnI GGCC 1 cut(s) 210
Bsp1286I GDGCHC 2 cut(s) 41, 60
BspACI CCGC 3 cut(s) 351, 355, 357
BspANI GGCC 1 cut(s) 210
BspCNI CTCAG 1 cut(s) 46
BspFNI CGCG 1 cut(s) 357
BspLI GGNNCC 1 cut(s) 23
BspMAI CTGCAG 1 cut(s) 382
BspT107I GGYRCC 1 cut(s) 21
BsrFI RCCGGY 1 cut(s) 123
BsrI ACTGG 4 cut(s) 161, 191, 284, 387
BssAI RCCGGY 1 cut(s) 123
BssECI CCNNGG 2 cut(s) 259, 355
Bst2UI CCWGG 2 cut(s) 261, 270
Bst4CI ACNGT 1 cut(s) 397
BstC8I GCNNGC 3 cut(s) 169, 355, 433
BstDEI CTNAG 1 cut(s) 54
BstDSI CCRYGG 1 cut(s) 355
BstFNI CGCG 1 cut(s) 357
BstMWI GCNNNNNNNGC 4 cut(s) 350, 419, 428, 437
BstNI CCWGG 2 cut(s) 261, 270
BstNSI RCATGY 1 cut(s) 171
BstSCI CCNGG 2 cut(s) 259, 268
BstSFI CTRYAG 1 cut(s) 378
BstSLI GKGCMC 1 cut(s) 41
BstUI CGCG 1 cut(s) 357
BstV1I GCAGC 4 cut(s) 334, 356, 392, 395
BstV2I GAAGAC 1 cut(s) 153
BsuRI GGCC 1 cut(s) 210
BtgI CCRYGG 1 cut(s) 355
BtsI GCAGTG 1 cut(s) 435
BtsIMutI CAGTG 5 cut(s) 41, 154, 393, 394, 435
Cac8I GCNNGC 3 cut(s) 169, 355, 433
Cfr10I RCCGGY 1 cut(s) 123
Cfr13I GGNCC 1 cut(s) 209
Cfr42I CCGCGG 1 cut(s) 358
Csp6I GTAC 2 cut(s) 175, 466
CspAI ACCGGT 1 cut(s) 123
CviAII CATG 1 cut(s) 168
CviQI GTAC 2 cut(s) 175, 466
DdeI CTNAG 1 cut(s) 54
DriI GACNNNNNGTC 1 cut(s) 157
Eam1105I GACNNNNNGTC 1 cut(s) 157
Ecl136II GAGCTC 1 cut(s) 58
Eco24I GRGCYC 1 cut(s) 60
Eco53kI GAGCTC 1 cut(s) 58
Eco57I CTGAAG 3 cut(s) 147, 348, 422
EcoICRI GAGCTC 1 cut(s) 58
EcoRII CCWGG 2 cut(s) 259, 268
EcoT38I GRGCYC 1 cut(s) 60
FaeI CATG 1 cut(s) 171
FaiI YATR 2 cut(s) 169, 314
FatI CATG 1 cut(s) 167
FauI CCCGC 1 cut(s) 362
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 6 cut(s) 345, 348, 351, 358, 381, 384
FriOI GRGCYC 1 cut(s) 60
Fsp4HI GCNGC 6 cut(s) 345, 348, 351, 358, 381, 384
GluI GCNGC 6 cut(s) 345, 348, 351, 358, 381, 384
HaeIII GGCC 1 cut(s) 210
HapII CCGG 1 cut(s) 124
Hin1II CATG 1 cut(s) 171
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 1 cut(s) 330
HinfI GANTC 2 cut(s) 84, 305
HpaII CCGG 1 cut(s) 124
HphI GGTGA 2 cut(s) 248, 254
Hpy166II GTNNAC 1 cut(s) 277
Hpy188I TCNGA 1 cut(s) 460
Hpy188III TCNNGA 3 cut(s) 14, 53, 400
Hpy8I GTNNAC 1 cut(s) 277
HpyAV CCTTC 2 cut(s) 333, 397
HpyCH4III ACNGT 1 cut(s) 397
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 2 cut(s) 200, 380
HpyF10VI GCNNNNNNNGC 4 cut(s) 350, 419, 428, 437
HpyF3I CTNAG 1 cut(s) 54
HpySE526I ACGT 1 cut(s) 135
Hsp92II CATG 1 cut(s) 171
KspI CCGCGG 1 cut(s) 358
Lsp1109I GCAGC 4 cut(s) 334, 356, 392, 395
LweI GCATC 1 cut(s) 369
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 1 cut(s) 131
MboII GAAGA 2 cut(s) 153, 468
MfeI CAATTG 1 cut(s) 204
MhlI GDGCHC 2 cut(s) 41, 60
MluCI AATT 1 cut(s) 204
MlyI GAGTC 1 cut(s) 78
MnlI CCTC 5 cut(s) 56, 238, 264, 454, 460
MseI TTAA 1 cut(s) 335
MslI CAYNNNNRTG 2 cut(s) 117, 261
MspA1I CMGCKG 2 cut(s) 347, 357
MspI CCGG 1 cut(s) 124
MspR9I CCNGG 2 cut(s) 261, 270
MunI CAATTG 1 cut(s) 204
MvaI CCWGG 2 cut(s) 261, 270
MvnI CGCG 1 cut(s) 357
MwoI GCNNNNNNNGC 4 cut(s) 350, 419, 428, 437
NlaIII CATG 1 cut(s) 171
NlaIV GGNNCC 1 cut(s) 23
NmuCI GTSAC 1 cut(s) 131
NspI RCATGY 1 cut(s) 171
OliI CACNNNNGTG 1 cut(s) 261
PaeI GCATGC 1 cut(s) 171
PfeI GAWTC 1 cut(s) 305
PinAI ACCGGT 1 cut(s) 123
PkrI GCNGC 6 cut(s) 346, 349, 352, 359, 382, 385
PleI GAGTC 1 cut(s) 78
PpsI GAGTC 1 cut(s) 78
Psp124BI GAGCTC 1 cut(s) 60
Psp6I CCWGG 2 cut(s) 259, 268
PspGI CCWGG 2 cut(s) 259, 268
PspN4I GGNNCC 1 cut(s) 23
PspPI GGNCC 1 cut(s) 209
PstI CTGCAG 1 cut(s) 382
PvuII CAGCTG 1 cut(s) 347
RsaI GTAC 2 cut(s) 176, 467
RsaNI GTAC 2 cut(s) 175, 466
RseI CAYNNNNRTG 2 cut(s) 117, 261
SacI GAGCTC 1 cut(s) 60
SacII CCGCGG 1 cut(s) 358
SalI GTCGAC 1 cut(s) 275
SaqAI TTAA 1 cut(s) 335
SatI GCNGC 6 cut(s) 345, 348, 351, 358, 381, 384
Sau96I GGNCC 1 cut(s) 209
ScaI AGTACT 1 cut(s) 467
SchI GAGTC 1 cut(s) 78
ScrFI CCNGG 2 cut(s) 261, 270
SduI GDGCHC 2 cut(s) 41, 60
SfaNI GCATC 1 cut(s) 369
SfcI CTRYAG 1 cut(s) 378
Sfr303I CCGCGG 1 cut(s) 358
SgrBI CCGCGG 1 cut(s) 358
SmiMI CAYNNNNRTG 2 cut(s) 117, 261
SmlI CTYRAG 2 cut(s) 59, 299
SmoI CTYRAG 2 cut(s) 59, 299
SphI GCATGC 1 cut(s) 171
Sse9I AATT 1 cut(s) 204
SsiI CCGC 3 cut(s) 351, 355, 357
SstI GAGCTC 1 cut(s) 60
StyD4I CCNGG 2 cut(s) 259, 268
TaaI ACNGT 1 cut(s) 397
TaiI ACGT 1 cut(s) 138
TaqI TCGA 2 cut(s) 146, 276
TasI AATT 1 cut(s) 204
TatI WGTACW 1 cut(s) 465
TauI GCSGC 2 cut(s) 353, 360
TfiI GAWTC 1 cut(s) 305
Tru1I TTAA 1 cut(s) 335
Tru9I TTAA 1 cut(s) 335
TscAI CASTG 5 cut(s) 41, 161, 394, 400, 442
TseFI GTSAC 1 cut(s) 131
TseI GCWGC 4 cut(s) 344, 347, 380, 383
Tsp45I GTSAC 1 cut(s) 131
TspRI CASTG 5 cut(s) 41, 161, 394, 400, 442
XceI RCATGY 1 cut(s) 171
XmiI GTMKAC 1 cut(s) 276
ZrmI AGTACT 1 cut(s) 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.