MD08G1121500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002864
Physical Location & Seq
Forward (+)
11112266 .. 11121843
9578 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1121500.v1.1.491

Sequence Viewer

Length: 480 bp
ATGGAGTGGCGTCCGTTGATAGCTATCGATGCGAACGTCCAGATGCAGAACCCTAAAGACGAAGCCATGTTCGACGAAACAACCATGGTCGATGATGATGAAGTAATGGGCAAATCTAAAGCTACCCTCAAACCAAAACCAAGTTGGTTCGATGATGATGATGACTACATTGGCCAGAATTGTGGCGATGAAGAGGAAGGTATACAAGAGGCGATTGGAGACGCAATGGCTCGACTCCAACTGCGAAAAAAGCGTAACTACTGTGAAGTTTGTTGTGAGGAAGTTGAGGACCACAAGAGTCACAATTGCCCATACTTTGTTTTGGTCCCAAGGGGCGCTCATGTTGGGGAACACTGTGATATAGTTTGCACAGAATGTGGTGAGCAAGTTTCTGAGCACGAAGGTGAGCTTAACGTAGACTATGAAGGACGTGCTATTTTGAAGTACTGTGATTGGTGTGAGGATTACCGTAGCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

160

Amino Acids

18.3

Weight (kDa)

4.4

Isoelectric Point (pI)

36.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 202, 417
AcoI YGGCCR 1 cut(s) 172
AcyI GRCGYC 1 cut(s) 10
AfaI GTAC 1 cut(s) 446
AgsI TTSAA 1 cut(s) 442
AjiI CACGTC 1 cut(s) 431
AleI CACNNNNGTG 1 cut(s) 402
AluBI AGCT 3 cut(s) 23, 122, 409
AluI AGCT 3 cut(s) 23, 122, 409
Alw21I GWGCWC 1 cut(s) 399
Alw26I GTCTC 1 cut(s) 213
AoxI GGCC 1 cut(s) 172
AspLEI GCGC 1 cut(s) 338
AspS9I GGNCC 2 cut(s) 289, 325
AsuHPI GGTGA 2 cut(s) 392, 416
AvaII GGWCC 2 cut(s) 289, 325
BalI TGGCCA 1 cut(s) 174
Bbv12I GWGCWC 1 cut(s) 399
BcoDI GTCTC 1 cut(s) 213
BfaI CTAG 1 cut(s) 478
BfoI RGCGCY 1 cut(s) 339
BmcAI AGTACT 1 cut(s) 446
Bme18I GGWCC 2 cut(s) 289, 325
BmgBI CACGTC 1 cut(s) 431
BmgT120I GGNCC 2 cut(s) 289, 325
BmiI GGNNCC 1 cut(s) 327
BmsI GCATC 2 cut(s) 19, 33
Bsa29I ATCGAT 1 cut(s) 27
BsaBI GATNNNNATC 1 cut(s) 23
BsaHI GRCGYC 1 cut(s) 10
BsaJI CCNNGG 2 cut(s) 84, 329
Bse3DI GCAATG 1 cut(s) 231
Bse8I GATNNNNATC 1 cut(s) 23
BseCI ATCGAT 1 cut(s) 27
BseDI CCNNGG 2 cut(s) 84, 329
BseJI GATNNNNATC 1 cut(s) 23
BseMI GCAATG 1 cut(s) 231
BseMII CTCAG 1 cut(s) 384
BshFI GGCC 1 cut(s) 174
BshVI ATCGAT 1 cut(s) 27
BsiHKAI GWGCWC 1 cut(s) 399
BslFI GGGAC 1 cut(s) 311
BsmAI GTCTC 1 cut(s) 213
BsmBI CGTCTC 1 cut(s) 213
BsmFI GGGAC 1 cut(s) 311
BsnI GGCC 1 cut(s) 174
Bsp1286I GDGCHC 1 cut(s) 399
Bsp19I CCATGG 1 cut(s) 84
BspANI GGCC 1 cut(s) 174
BspCNI CTCAG 1 cut(s) 385
BspDI ATCGAT 1 cut(s) 27
BspLI GGNNCC 1 cut(s) 327
BsrDI GCAATG 1 cut(s) 231
BssECI CCNNGG 2 cut(s) 84, 329
BssNAI GTATAC 1 cut(s) 203
BssNI GRCGYC 1 cut(s) 10
BssT1I CCWWGG 2 cut(s) 84, 329
Bst1107I GTATAC 1 cut(s) 203
Bst4CI ACNGT 4 cut(s) 263, 356, 449, 470
Bst6I CTCTTC 1 cut(s) 186
BstACI GRCGYC 1 cut(s) 10
BstDEI CTNAG 1 cut(s) 393
BstDSI CCRYGG 1 cut(s) 84
BstH2I RGCGCY 1 cut(s) 339
BstHHI GCGC 1 cut(s) 338
BstMAI GTCTC 1 cut(s) 213
BstMWI GCNNNNNNNGC 2 cut(s) 29, 250
BstXI CCANNNNNNTGG 1 cut(s) 182
BstZ17I GTATAC 1 cut(s) 203
Bsu15I ATCGAT 1 cut(s) 27
BsuRI GGCC 1 cut(s) 174
BsuTUI ATCGAT 1 cut(s) 27
BtgI CCRYGG 1 cut(s) 84
BtgZI GCGATG 1 cut(s) 201
BtrI CACGTC 1 cut(s) 431
BtsIMutI CAGTG 1 cut(s) 352
CfoI GCGC 1 cut(s) 338
Cfr13I GGNCC 2 cut(s) 289, 325
ClaI ATCGAT 1 cut(s) 27
CseI GACGC 1 cut(s) 230
Csp6I GTAC 1 cut(s) 445
CviAII CATG 3 cut(s) 67, 85, 341
CviJI RGCY 7 cut(s) 23, 65, 122, 174, 230, 409, 474
CviKI_1 RGCY 7 cut(s) 23, 65, 122, 174, 230, 409, 474
CviQI GTAC 1 cut(s) 445
DdeI CTNAG 1 cut(s) 393
EaeI YGGCCR 1 cut(s) 172
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
Eco130I CCWWGG 2 cut(s) 84, 329
Eco47I GGWCC 2 cut(s) 289, 325
EcoT14I CCWWGG 2 cut(s) 84, 329
ErhI CCWWGG 2 cut(s) 84, 329
Esp3I CGTCTC 1 cut(s) 213
FaeI CATG 3 cut(s) 70, 88, 344
FaiI YATR 7 cut(s) 68, 86, 203, 313, 342, 362, 423
FalI AAGNNNNNCTT 2 cut(s) 393, 425
FaqI GGGAC 1 cut(s) 311
FatI CATG 3 cut(s) 66, 84, 340
FblI GTMKAC 2 cut(s) 202, 417
FspBI CTAG 1 cut(s) 478
GlaI GCGC 1 cut(s) 337
HaeII RGCGCY 1 cut(s) 339
HaeIII GGCC 1 cut(s) 174
HgaI GACGC 1 cut(s) 230
HhaI GCGC 1 cut(s) 338
Hin1I GRCGYC 1 cut(s) 10
Hin1II CATG 3 cut(s) 70, 88, 344
Hin6I GCGC 1 cut(s) 336
HinP1I GCGC 1 cut(s) 336
HinfI GANTC 2 cut(s) 234, 298
HphI GGTGA 2 cut(s) 392, 416
Hpy166II GTNNAC 2 cut(s) 203, 418
Hpy188I TCNGA 1 cut(s) 394
Hpy188III TCNNGA 1 cut(s) 40
Hpy8I GTNNAC 2 cut(s) 203, 418
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 3 cut(s) 191, 395, 419
HpyCH4III ACNGT 4 cut(s) 263, 356, 449, 470
HpyCH4IV ACGT 3 cut(s) 36, 414, 430
HpyCH4V TGCA 2 cut(s) 46, 369
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 250
HpyF3I CTNAG 1 cut(s) 393
HpySE526I ACGT 3 cut(s) 36, 414, 430
Hsp92I GRCGYC 1 cut(s) 10
Hsp92II CATG 3 cut(s) 70, 88, 344
HspAI GCGC 1 cut(s) 336
LpnPI CCDG 2 cut(s) 53, 188
LweI GCATC 2 cut(s) 19, 33
MaeI CTAG 1 cut(s) 478
MaeII ACGT 3 cut(s) 36, 414, 430
MaeIII GTNAC 2 cut(s) 254, 299
MboII GAAGA 1 cut(s) 203
MfeI CAATTG 1 cut(s) 304
MhlI GDGCHC 1 cut(s) 399
MlsI TGGCCA 1 cut(s) 174
MluCI AATT 2 cut(s) 178, 304
MluNI TGGCCA 1 cut(s) 174
MlyI GAGTC 2 cut(s) 228, 307
MmeI TCCRAC 1 cut(s) 262
MnlI CCTC 6 cut(s) 137, 187, 202, 271, 280, 454
Mox20I TGGCCA 1 cut(s) 174
MscI TGGCCA 1 cut(s) 174
MseI TTAA 1 cut(s) 411
MslI CAYNNNNRTG 1 cut(s) 402
Msp20I TGGCCA 1 cut(s) 174
MunI CAATTG 1 cut(s) 304
MwoI GCNNNNNNNGC 2 cut(s) 29, 250
NcoI CCATGG 1 cut(s) 84
NlaIII CATG 3 cut(s) 70, 88, 344
NlaIV GGNNCC 1 cut(s) 327
NmuCI GTSAC 1 cut(s) 299
OliI CACNNNNGTG 1 cut(s) 402
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PleI GAGTC 2 cut(s) 228, 306
PpsI GAGTC 2 cut(s) 228, 306
PspN4I GGNNCC 1 cut(s) 327
PspPI GGNCC 2 cut(s) 289, 325
RsaI GTAC 1 cut(s) 446
RsaNI GTAC 1 cut(s) 445
RseI CAYNNNNRTG 1 cut(s) 402
SaqAI TTAA 1 cut(s) 411
Sau96I GGNCC 2 cut(s) 289, 325
ScaI AGTACT 1 cut(s) 446
SchI GAGTC 2 cut(s) 228, 307
SduI GDGCHC 1 cut(s) 399
SetI ASST 8 cut(s) 25, 39, 124, 202, 406, 411, 417, 433
SfaNI GCATC 2 cut(s) 19, 33
SinI GGWCC 2 cut(s) 289, 325
SmiMI CAYNNNNRTG 1 cut(s) 402
Sse9I AATT 2 cut(s) 178, 304
SspMI CTAG 1 cut(s) 478
StyI CCWWGG 2 cut(s) 84, 329
TaaI ACNGT 4 cut(s) 263, 356, 449, 470
TaiI ACGT 3 cut(s) 39, 417, 433
TaqI TCGA 5 cut(s) 27, 72, 90, 150, 232
TasI AATT 2 cut(s) 178, 304
TatI WGTACW 1 cut(s) 444
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TscAI CASTG 1 cut(s) 359
TseFI GTSAC 1 cut(s) 299
Tsp45I GTSAC 1 cut(s) 299
TspDTI ATGAA 3 cut(s) 114, 204, 438
TspRI CASTG 1 cut(s) 359
VpaK11BI GGWCC 2 cut(s) 289, 325
XcmI CCANNNNNNNNNTGG 1 cut(s) 141
XmiI GTMKAC 2 cut(s) 202, 417
XspI CTAG 1 cut(s) 478
ZrmI AGTACT 1 cut(s) 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.