Rh5DG435100

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0002864
Physical Location & Seq
Forward (+)
69465313 .. 69466833
1521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG435100.1

Sequence Viewer

Length: 729 bp
ATGCCAGTGCCAATCCCTAACCGCAGAGCCATGGGCCAGGAACTTGAAGAACAAGCCGCGCCGCCGGTCATCCTCCGATGGCAAGATCTGGGCGAGTTGCCGGAAGGCGTGCTGATTATTAAGGCTCCCGAAGATTATACCCCTCCTCGTGGGAAAGGGAAAGGGAAAGGGAACAAGGGGAAAGGGAACCAAGGGAGAGTGAAAGCACAGGTAAGTGATGATAAGGAAGGACCACCATTGGCTAATTATATCAAACTGAGTGAGTATTCCTTTGCTTGTAAGATGTGTCCGAATCCAGATTGGCACCGGACTGTTCACTGCCCCAACCTCACTCCTGAGCTCAAGGAAAGGCTAAAAGTAGCAAGAAGCAGGGATCGAAAACTTAAAGCTTTCCACCCTGATGACATGGCTTGTCATATTTGTCTAAAGACAGACCACTGGTCTTCAGAATGCTCATACCTGCTCGAATATCCGAGGGGTGCAATTGTTGGCCGTTTTGCTCAAATAATTTGCTTGTGTTGTGGTGAAGAGATGCCCCACCCTGGTGTACCTGGTCTTGACTGGAAAGCAAGAGTTATTCTCAAGCCTGCTGTTACGTATCCCAGTATCAAGGAACAGCTGATGGAACTGACACTGGCATCAGAAGGCGCAGCTGATGGCAATGGCAACAACACAAGTACATCATCAGAGGGCGCAGCTTATGGCACTGGCATCAAGGCAAGTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

242

Amino Acids

26.49

Weight (kDa)

8.32

Isoelectric Point (pI)

44.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 468
AccB1I GGYRCC 1 cut(s) 303
AccII CGCG 1 cut(s) 59
AciI CCGC 3 cut(s) 22, 57, 62
AclWI GGATC 1 cut(s) 381
AcoI YGGCCR 1 cut(s) 490
AcuI CTGAAG 1 cut(s) 429
AfaI GTAC 2 cut(s) 549, 679
AfiI CCNNNNNNNGG 2 cut(s) 149, 542
AgsI TTSAA 1 cut(s) 47
AhdI GACNNNNNGTC 1 cut(s) 439
AjnI CCWGG 3 cut(s) 36, 541, 550
AleI CACNNNNGTG 1 cut(s) 543
AluBI AGCT 5 cut(s) 340, 389, 619, 653, 698
AluI AGCT 5 cut(s) 340, 389, 619, 653, 698
Alw21I GWGCWC 1 cut(s) 342
AlwI GGATC 1 cut(s) 381
AoxI GGCC 2 cut(s) 34, 490
ApeKI GCWGC 2 cut(s) 650, 695
AspLEI GCGC 3 cut(s) 61, 650, 695
AspS9I GGNCC 2 cut(s) 34, 230
AsuHPI GGTGA 1 cut(s) 536
AvaII GGWCC 1 cut(s) 230
BanI GGYRCC 1 cut(s) 303
BanII GRGCYC 1 cut(s) 342
BauI CACGAG 1 cut(s) 147
BbsI GAAGAC 1 cut(s) 435
Bbv12I GWGCWC 1 cut(s) 342
BbvI GCAGC 2 cut(s) 662, 707
BccI CCATC 3 cut(s) 72, 616, 650
BceAI ACGGC 1 cut(s) 477
BciT130I CCWGG 3 cut(s) 38, 543, 552
BciVI GTATCC 1 cut(s) 609
BfuAI ACCTGC 1 cut(s) 468
BfuI GTATCC 1 cut(s) 609
BglII AGATCT 1 cut(s) 85
BisI GCNGC 4 cut(s) 57, 62, 651, 696
BlsI GCNGC 4 cut(s) 58, 63, 652, 697
Bme1390I CCNGG 3 cut(s) 38, 543, 552
Bme18I GGWCC 1 cut(s) 230
BmeRI GACNNNNNGTC 1 cut(s) 439
BmgT120I GGNCC 2 cut(s) 34, 230
BmiI GGNNCC 3 cut(s) 126, 188, 305
BmrFI CCNGG 3 cut(s) 38, 543, 552
BmrI ACTGGG 1 cut(s) 597
BmsI GCATC 3 cut(s) 522, 647, 720
BmuI ACTGGG 1 cut(s) 597
BpiI GAAGAC 1 cut(s) 435
BplI GAGNNNNNCTC 2 cut(s) 564, 596
Bpu10I CCTNAGC 1 cut(s) 336
BpuEI CTTGAG 2 cut(s) 326, 566
BsaAI YACGTR 1 cut(s) 597
BsaJI CCNNGG 4 cut(s) 30, 190, 473, 541
BsaWI WCCGGW 1 cut(s) 306
Bsc4I CCNNNNNNNGG 2 cut(s) 149, 542
Bse118I RCCGGY 1 cut(s) 64
Bse1I ACTGG 6 cut(s) 5, 443, 566, 603, 639, 712
Bse3DI GCAATG 1 cut(s) 667
BseBI CCWGG 3 cut(s) 38, 543, 552
BseDI CCNNGG 4 cut(s) 30, 190, 473, 541
BseGI GGATG 1 cut(s) 69
BseLI CCNNNNNNNGG 2 cut(s) 149, 542
BseMI GCAATG 1 cut(s) 667
BseMII CTCAG 2 cut(s) 248, 327
BseNI ACTGG 6 cut(s) 5, 443, 566, 603, 639, 712
BseRI GAGGAG 1 cut(s) 135
BseXI GCAGC 2 cut(s) 662, 707
Bsh1236I CGCG 1 cut(s) 59
BshFI GGCC 2 cut(s) 36, 492
BshNI GGYRCC 1 cut(s) 303
BsiHKAI GWGCWC 1 cut(s) 342
BsiSI CCGG 3 cut(s) 65, 101, 307
BslI CCNNNNNNNGG 2 cut(s) 149, 542
BsmI GAATGC 1 cut(s) 455
BsnI GGCC 2 cut(s) 36, 492
Bsp1286I GDGCHC 1 cut(s) 342
Bsp143I GATC 2 cut(s) 85, 373
Bsp19I CCATGG 1 cut(s) 30
BspACI CCGC 3 cut(s) 22, 57, 62
BspANI GGCC 2 cut(s) 36, 492
BspCNI CTCAG 2 cut(s) 249, 328
BspFNI CGCG 1 cut(s) 59
BspLI GGNNCC 3 cut(s) 126, 188, 305
BspMI ACCTGC 1 cut(s) 468
BspPI GGATC 1 cut(s) 381
BspT107I GGYRCC 1 cut(s) 303
BsrDI GCAATG 1 cut(s) 667
BsrFI RCCGGY 1 cut(s) 64
BsrI ACTGG 6 cut(s) 5, 443, 566, 603, 639, 712
BssAI RCCGGY 1 cut(s) 64
BssECI CCNNGG 4 cut(s) 30, 190, 473, 541
BssMI GATC 2 cut(s) 85, 373
BssSI CACGAG 1 cut(s) 147
BssT1I CCWWGG 2 cut(s) 30, 190
Bst2BI CACGAG 1 cut(s) 147
Bst2UI CCWGG 3 cut(s) 38, 543, 552
Bst4CI ACNGT 1 cut(s) 313
Bst6I CTCTTC 1 cut(s) 522
BstBAI YACGTR 1 cut(s) 597
BstC8I GCNNGC 2 cut(s) 110, 588
BstDEI CTNAG 2 cut(s) 257, 336
BstDSI CCRYGG 1 cut(s) 30
BstF5I GGATG 1 cut(s) 69
BstFNI CGCG 1 cut(s) 59
BstHHI GCGC 3 cut(s) 61, 650, 695
BstKTI GATC 2 cut(s) 88, 376
BstMBI GATC 2 cut(s) 85, 373
BstNI CCWGG 3 cut(s) 38, 543, 552
BstSCI CCNGG 3 cut(s) 36, 541, 550
BstSNI TACGTA 1 cut(s) 597
BstUI CGCG 1 cut(s) 59
BstV1I GCAGC 2 cut(s) 662, 707
BstV2I GAAGAC 1 cut(s) 435
BstX2I RGATCY 1 cut(s) 85
BstYI RGATCY 1 cut(s) 85
BsuI GTATCC 1 cut(s) 609
BsuRI GGCC 2 cut(s) 36, 492
BtgI CCRYGG 1 cut(s) 30
BtsCI GGATG 1 cut(s) 69
BtsI GCAGTG 1 cut(s) 316
BtsIMutI CAGTG 5 cut(s) 12, 316, 436, 632, 705
BveI ACCTGC 1 cut(s) 468
Cac8I GCNNGC 2 cut(s) 110, 588
CfoI GCGC 3 cut(s) 61, 650, 695
Cfr10I RCCGGY 1 cut(s) 64
Cfr13I GGNCC 2 cut(s) 34, 230
CsiI ACCWGGT 1 cut(s) 550
Csp6I GTAC 2 cut(s) 548, 678
CspCI CAANNNNNGTGG 1 cut(s) 703
CviAII CATG 2 cut(s) 31, 406
CviQI GTAC 2 cut(s) 548, 678
DdeI CTNAG 2 cut(s) 257, 336
DpnI GATC 2 cut(s) 87, 375
DpnII GATC 2 cut(s) 85, 373
DriI GACNNNNNGTC 1 cut(s) 439
EaeI YGGCCR 1 cut(s) 490
Eam1104I CTCTTC 1 cut(s) 522
Eam1105I GACNNNNNGTC 1 cut(s) 439
EarI CTCTTC 1 cut(s) 522
Ecl136II GAGCTC 1 cut(s) 340
Eco105I TACGTA 1 cut(s) 597
Eco130I CCWWGG 2 cut(s) 30, 190
Eco24I GRGCYC 1 cut(s) 342
Eco47I GGWCC 1 cut(s) 230
Eco53kI GAGCTC 1 cut(s) 340
Eco57I CTGAAG 1 cut(s) 429
EcoICRI GAGCTC 1 cut(s) 340
EcoRII CCWGG 3 cut(s) 36, 541, 550
EcoT14I CCWWGG 2 cut(s) 30, 190
EcoT38I GRGCYC 1 cut(s) 342
ErhI CCWWGG 2 cut(s) 30, 190
FaeI CATG 2 cut(s) 34, 409
FaiI YATR 7 cut(s) 32, 138, 249, 407, 417, 457, 702
FatI CATG 2 cut(s) 30, 405
Fnu4HI GCNGC 4 cut(s) 57, 62, 651, 696
FokI GGATG 1 cut(s) 56
FriOI GRGCYC 1 cut(s) 342
Fsp4HI GCNGC 4 cut(s) 57, 62, 651, 696
GlaI GCGC 3 cut(s) 60, 649, 694
GluI GCNGC 4 cut(s) 57, 62, 651, 696
HaeIII GGCC 2 cut(s) 36, 492
HapII CCGG 3 cut(s) 65, 101, 307
HhaI GCGC 3 cut(s) 61, 650, 695
Hin1II CATG 2 cut(s) 34, 409
Hin6I GCGC 3 cut(s) 59, 648, 693
HinP1I GCGC 3 cut(s) 59, 648, 693
HindIII AAGCTT 1 cut(s) 387
HinfI GANTC 1 cut(s) 292
HpaII CCGG 3 cut(s) 65, 101, 307
HphI GGTGA 1 cut(s) 536
Hpy166II GTNNAC 2 cut(s) 316, 548
Hpy188I TCNGA 6 cut(s) 77, 291, 448, 474, 643, 688
Hpy188III TCNNGA 4 cut(s) 128, 296, 335, 557
Hpy8I GTNNAC 2 cut(s) 316, 548
HpyAV CCTTC 3 cut(s) 98, 221, 638
HpyCH4III ACNGT 1 cut(s) 313
HpyCH4IV ACGT 1 cut(s) 596
HpyCH4V TGCA 1 cut(s) 482
HpyF3I CTNAG 2 cut(s) 257, 336
HpySE526I ACGT 1 cut(s) 596
Hsp92II CATG 2 cut(s) 34, 409
HspAI GCGC 3 cut(s) 59, 648, 693
Kzo9I GATC 2 cut(s) 85, 373
LmnI GCTCC 1 cut(s) 130
Lsp1109I GCAGC 2 cut(s) 662, 707
LweI GCATC 3 cut(s) 522, 647, 720
MabI ACCWGGT 1 cut(s) 550
MaeII ACGT 1 cut(s) 596
MaeIII GTNAC 1 cut(s) 592
MalI GATC 2 cut(s) 87, 375
MboI GATC 2 cut(s) 85, 373
MboII GAAGA 4 cut(s) 59, 143, 435, 539
MfeI CAATTG 1 cut(s) 483
MflI RGATCY 1 cut(s) 85
MhlI GDGCHC 1 cut(s) 342
MluCI AATT 3 cut(s) 244, 483, 507
MnlI CCTC 6 cut(s) 83, 153, 156, 338, 468, 682
MseI TTAA 2 cut(s) 120, 384
MslI CAYNNNNRTG 2 cut(s) 399, 543
MspA1I CMGCKG 2 cut(s) 619, 653
MspI CCGG 3 cut(s) 65, 101, 307
MspR9I CCNGG 3 cut(s) 38, 543, 552
MunI CAATTG 1 cut(s) 483
Mva1269I GAATGC 1 cut(s) 455
MvaI CCWGG 3 cut(s) 38, 543, 552
MvnI CGCG 1 cut(s) 59
NcoI CCATGG 1 cut(s) 30
NdeII GATC 2 cut(s) 85, 373
NlaIII CATG 2 cut(s) 34, 409
NlaIV GGNNCC 3 cut(s) 126, 188, 305
OliI CACNNNNGTG 1 cut(s) 543
PctI GAATGC 1 cut(s) 455
PfeI GAWTC 1 cut(s) 292
PkrI GCNGC 4 cut(s) 58, 63, 652, 697
Ppu21I YACGTR 1 cut(s) 597
Psp124BI GAGCTC 1 cut(s) 342
Psp6I CCWGG 3 cut(s) 36, 541, 550
PspGI CCWGG 3 cut(s) 36, 541, 550
PspN4I GGNNCC 3 cut(s) 126, 188, 305
PspPI GGNCC 2 cut(s) 34, 230
PsuI RGATCY 1 cut(s) 85
PvuII CAGCTG 2 cut(s) 619, 653
RsaI GTAC 2 cut(s) 549, 679
RsaNI GTAC 2 cut(s) 548, 678
RseI CAYNNNNRTG 2 cut(s) 399, 543
SacI GAGCTC 1 cut(s) 342
SaqAI TTAA 2 cut(s) 120, 384
SatI GCNGC 4 cut(s) 57, 62, 651, 696
Sau3AI GATC 2 cut(s) 85, 373
Sau96I GGNCC 2 cut(s) 34, 230
ScrFI CCNGG 3 cut(s) 38, 543, 552
SduI GDGCHC 1 cut(s) 342
SexAI ACCWGGT 1 cut(s) 550
SfaNI GCATC 3 cut(s) 522, 647, 720
SinI GGWCC 1 cut(s) 230
SmiMI CAYNNNNRTG 2 cut(s) 399, 543
SmlI CTYRAG 2 cut(s) 341, 581
SmoI CTYRAG 2 cut(s) 341, 581
SnaBI TACGTA 1 cut(s) 597
Sse9I AATT 3 cut(s) 244, 483, 507
SsiI CCGC 3 cut(s) 22, 57, 62
SstI GAGCTC 1 cut(s) 342
StyD4I CCNGG 3 cut(s) 36, 541, 550
StyI CCWWGG 2 cut(s) 30, 190
TaaI ACNGT 1 cut(s) 313
TaiI ACGT 1 cut(s) 599
TaqI TCGA 2 cut(s) 376, 465
TasI AATT 3 cut(s) 244, 483, 507
TatI WGTACW 1 cut(s) 677
TauI GCSGC 2 cut(s) 59, 64
TfiI GAWTC 1 cut(s) 292
Tru1I TTAA 2 cut(s) 120, 384
Tru9I TTAA 2 cut(s) 120, 384
TscAI CASTG 5 cut(s) 12, 323, 443, 639, 712
TseI GCWGC 2 cut(s) 650, 695
TspRI CASTG 5 cut(s) 12, 323, 443, 639, 712
VpaK11BI GGWCC 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.