Rh5DG391900

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0002864
Physical Location & Seq
Reverse (-)
61317949 .. 61323815
5867 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG391900.1

Sequence Viewer

Length: 363 bp
ATGGAACACATATGGCGGAAGGCAGGCACAATTGCGGTGGCGCAAGAGCTCAAGGAAATGCTAAAAGAAGATAGAAGCAAGTTTGGAACATGTAGGCCTCTTCGCCCTGAGGACATGGGTTGTCATATATGTCTGAAGAGAGACCACTGGCCTATATCATGCCCCTACCAGCTCGTATATCCTCCGGGTGCACCTGTTGGCCCTTTTGCTGAGATAGTTTGTTTGTGTTGTGGCGAGGAGAAACCCCACCCTGGTGAGCCTGGTGTTGACTGGAAAGCAAGAGCCATTCTCAAGATTTCTCCAACTTATCCCAGTTTCGACTCATATGCAGCGGTGGGTGCAGCTGATGGCGCAAGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

120

Amino Acids

13.2

Weight (kDa)

6.4

Isoelectric Point (pI)

55.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 16, 35, 332
AcuI CTGAAG 1 cut(s) 155
AfiI CCNNNNNNNGG 1 cut(s) 251
AflIII ACRYGT 1 cut(s) 89
AjnI CCWGG 2 cut(s) 250, 259
AleI CACNNNNGTG 1 cut(s) 252
AluBI AGCT 3 cut(s) 49, 172, 344
AluI AGCT 3 cut(s) 49, 172, 344
Alw21I GWGCWC 2 cut(s) 51, 193
Alw26I GTCTC 1 cut(s) 135
Alw44I GTGCAC 1 cut(s) 189
AoxI GGCC 3 cut(s) 95, 149, 199
ApaLI GTGCAC 1 cut(s) 189
ApeKI GCWGC 2 cut(s) 329, 341
AspLEI GCGC 2 cut(s) 43, 353
AspS9I GGNCC 1 cut(s) 200
AsuC2I CCSGG 1 cut(s) 186
AsuHPI GGTGA 1 cut(s) 266
AxyI CCTNAGG 1 cut(s) 108
BaeGI GKGCMC 1 cut(s) 193
BanII GRGCYC 1 cut(s) 51
Bbv12I GWGCWC 2 cut(s) 51, 193
BbvI GCAGC 2 cut(s) 341, 353
BccI CCATC 1 cut(s) 341
BcgI CGANNNNNNTGC 2 cut(s) 308, 342
BciT130I CCWGG 2 cut(s) 252, 261
BcnI CCSGG 1 cut(s) 186
BcoDI GTCTC 1 cut(s) 135
BisI GCNGC 2 cut(s) 330, 342
BlsI GCNGC 2 cut(s) 331, 343
Bme1390I CCNGG 3 cut(s) 186, 252, 261
BmgT120I GGNCC 1 cut(s) 200
BmrFI CCNGG 3 cut(s) 186, 252, 261
BmrI ACTGGG 1 cut(s) 306
BmuI ACTGGG 1 cut(s) 306
BplI GAGNNNNNCTC 2 cut(s) 273, 305
BpuEI CTTGAG 2 cut(s) 35, 275
BpuMI CCSGG 1 cut(s) 186
BsaI GGTCTC 1 cut(s) 135
BsaJI CCNNGG 1 cut(s) 250
Bsc4I CCNNNNNNNGG 1 cut(s) 251
Bse1I ACTGG 3 cut(s) 152, 275, 312
Bse21I CCTNAGG 1 cut(s) 108
BseBI CCWGG 2 cut(s) 252, 261
BseDI CCNNGG 1 cut(s) 250
BseLI CCNNNNNNNGG 1 cut(s) 251
BseMII CTCAG 2 cut(s) 99, 201
BseNI ACTGG 3 cut(s) 152, 275, 312
BseRI GAGGAG 1 cut(s) 251
BseSI GKGCMC 1 cut(s) 193
BseXI GCAGC 2 cut(s) 341, 353
BsgI GTGCAG 1 cut(s) 360
BshFI GGCC 3 cut(s) 97, 151, 201
BsiHKAI GWGCWC 2 cut(s) 51, 193
BsiSI CCGG 1 cut(s) 185
BslI CCNNNNNNNGG 1 cut(s) 251
BsmAI GTCTC 1 cut(s) 135
BsnI GGCC 3 cut(s) 97, 151, 201
Bso31I GGTCTC 1 cut(s) 135
Bsp1286I GDGCHC 2 cut(s) 51, 193
BspACI CCGC 3 cut(s) 16, 35, 332
BspANI GGCC 3 cut(s) 97, 151, 201
BspCNI CTCAG 2 cut(s) 100, 202
BspTNI GGTCTC 1 cut(s) 135
BsrI ACTGG 3 cut(s) 152, 275, 312
BssECI CCNNGG 1 cut(s) 250
Bst2UI CCWGG 2 cut(s) 252, 261
Bst6I CTCTTC 2 cut(s) 105, 131
BstC8I GCNNGC 1 cut(s) 25
BstDEI CTNAG 2 cut(s) 108, 210
BstHHI GCGC 2 cut(s) 43, 353
BstMAI GTCTC 1 cut(s) 135
BstMWI GCNNNNNNNGC 2 cut(s) 338, 350
BstNI CCWGG 2 cut(s) 252, 261
BstNSI RCATGY 1 cut(s) 93
BstSCI CCNGG 3 cut(s) 184, 250, 259
BstSLI GKGCMC 1 cut(s) 193
BstV1I GCAGC 2 cut(s) 341, 353
Bsu36I CCTNAGG 1 cut(s) 108
BsuRI GGCC 3 cut(s) 97, 151, 201
BtsIMutI CAGTG 1 cut(s) 145
Cac8I GCNNGC 1 cut(s) 25
CfoI GCGC 2 cut(s) 43, 353
Cfr13I GGNCC 1 cut(s) 200
CspCI CAANNNNNGTGG 2 cut(s) 18, 53
CviAII CATG 3 cut(s) 90, 115, 159
CviJI RGCY 8 cut(s) 49, 97, 151, 172, 201, 259, 284, 344
CviKI_1 RGCY 8 cut(s) 49, 97, 151, 172, 201, 259, 284, 344
DdeI CTNAG 2 cut(s) 108, 210
Eam1104I CTCTTC 2 cut(s) 105, 131
EarI CTCTTC 2 cut(s) 105, 131
EciI GGCGGA 1 cut(s) 31
Ecl136II GAGCTC 1 cut(s) 49
Eco147I AGGCCT 1 cut(s) 97
Eco24I GRGCYC 1 cut(s) 51
Eco31I GGTCTC 1 cut(s) 135
Eco53kI GAGCTC 1 cut(s) 49
Eco57I CTGAAG 1 cut(s) 155
Eco81I CCTNAGG 1 cut(s) 108
EcoICRI GAGCTC 1 cut(s) 49
EcoRII CCWGG 2 cut(s) 250, 259
EcoT38I GRGCYC 1 cut(s) 51
FaeI CATG 3 cut(s) 93, 118, 162
FatI CATG 3 cut(s) 89, 114, 158
FauNDI CATATG 2 cut(s) 11, 325
Fnu4HI GCNGC 2 cut(s) 330, 342
FriOI GRGCYC 1 cut(s) 51
Fsp4HI GCNGC 2 cut(s) 330, 342
GlaI GCGC 2 cut(s) 42, 352
GluI GCNGC 2 cut(s) 330, 342
HaeIII GGCC 3 cut(s) 97, 151, 201
HapII CCGG 1 cut(s) 185
HhaI GCGC 2 cut(s) 43, 353
Hin1II CATG 3 cut(s) 93, 118, 162
Hin6I GCGC 2 cut(s) 41, 351
HinP1I GCGC 2 cut(s) 41, 351
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HinfI GANTC 1 cut(s) 320
HpaII CCGG 1 cut(s) 185
HphI GGTGA 1 cut(s) 266
Hpy166II GTNNAC 2 cut(s) 191, 268
Hpy188I TCNGA 1 cut(s) 135
Hpy188III TCNNGA 1 cut(s) 292
Hpy8I GTNNAC 2 cut(s) 191, 268
HpyAV CCTTC 1 cut(s) 13
HpyCH4V TGCA 3 cut(s) 191, 329, 341
HpyF10VI GCNNNNNNNGC 2 cut(s) 338, 350
HpyF3I CTNAG 2 cut(s) 108, 210
Hsp92II CATG 3 cut(s) 93, 118, 162
HspAI GCGC 2 cut(s) 41, 351
Lsp1109I GCAGC 2 cut(s) 341, 353
MboII GAAGA 3 cut(s) 80, 92, 148
MfeI CAATTG 1 cut(s) 30
MhlI GDGCHC 2 cut(s) 51, 193
MluCI AATT 1 cut(s) 30
MlyI GAGTC 1 cut(s) 314
MmeI TCCRAC 1 cut(s) 326
MnlI CCTC 4 cut(s) 103, 108, 192, 229
MslI CAYNNNNRTG 1 cut(s) 252
MspA1I CMGCKG 2 cut(s) 332, 344
MspI CCGG 1 cut(s) 185
MspR9I CCNGG 3 cut(s) 186, 252, 261
MunI CAATTG 1 cut(s) 30
MvaI CCWGG 2 cut(s) 252, 261
MwoI GCNNNNNNNGC 2 cut(s) 338, 350
NciI CCSGG 1 cut(s) 186
NdeI CATATG 2 cut(s) 11, 325
NlaIII CATG 3 cut(s) 93, 118, 162
NspI RCATGY 1 cut(s) 93
OliI CACNNNNGTG 1 cut(s) 252
PceI AGGCCT 1 cut(s) 97
PciI ACATGT 1 cut(s) 89
PkrI GCNGC 2 cut(s) 331, 343
PleI GAGTC 1 cut(s) 314
PpsI GAGTC 1 cut(s) 314
PscI ACATGT 1 cut(s) 89
Psp124BI GAGCTC 1 cut(s) 51
Psp6I CCWGG 2 cut(s) 250, 259
PspGI CCWGG 2 cut(s) 250, 259
PspPI GGNCC 1 cut(s) 200
PvuII CAGCTG 1 cut(s) 344
RseI CAYNNNNRTG 1 cut(s) 252
SacI GAGCTC 1 cut(s) 51
SatI GCNGC 2 cut(s) 330, 342
Sau96I GGNCC 1 cut(s) 200
SchI GAGTC 1 cut(s) 314
ScrFI CCNGG 3 cut(s) 186, 252, 261
SduI GDGCHC 2 cut(s) 51, 193
SetI ASST 4 cut(s) 51, 174, 196, 346
SmiMI CAYNNNNRTG 1 cut(s) 252
SmlI CTYRAG 2 cut(s) 50, 290
SmoI CTYRAG 2 cut(s) 50, 290
Sse9I AATT 1 cut(s) 30
SseBI AGGCCT 1 cut(s) 97
SsiI CCGC 3 cut(s) 16, 35, 332
SstI GAGCTC 1 cut(s) 51
StuI AGGCCT 1 cut(s) 97
StyD4I CCNGG 3 cut(s) 184, 250, 259
TaqI TCGA 1 cut(s) 318
TasI AATT 1 cut(s) 30
TscAI CASTG 1 cut(s) 152
TseI GCWGC 2 cut(s) 329, 341
TspRI CASTG 1 cut(s) 152
VneI GTGCAC 1 cut(s) 189
XceI RCATGY 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.