FvH4_3g36540

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000624
Physical Location & Seq
Forward (+)
31293469 .. 31298271
4803 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g36540.t1

Sequence Viewer

Length: 1128 bp
ATGGAGGAGGACATGGAGCCAAAGCCGTGCATGTTCCATGACCCTTTGGTTCTTCAAGCCACTGAGGAGTCTCTTTATGAGGTTATTGGAGGAGGGAAGTGCTCAACTTCCCAAGACCAAAACCCTCATTGGGTTGATCAAAATGAGTATGGAAATGGAAATGTGATGGTGGGTTCTTATAGGAGTGAGCTTATGGTTCCTGATGCGGTCAGTAAGAACGAATTGTTAGTGCAATTTGACCGAGGGGCAGAGGATGGTTGCAAGTTTCTTCCTAGAGGTCAGTTGATTACTGCAGAGGGCAACAAGACATATGCTGTGGCGAATAGTAAGGCAGGAAATGTGATTGTTACGACCCAGAAGAATGGCAGTGAGCATGTTCATGTTGGTTCAAGAGGAAAGACGAGTCATCAGAGGGAGGAGATAGATTTGGAGGATGGGAAGATTAACAAGCAATCGGCGGTTTATATGGATGATGTGGAGGGTGAGCTGTCTGATAGTTTCGGGAAGGCGTTGCTCAGCTGTAAGGCGGTCGAGAGTAACGAGGATCAAGTCTGTCAGAATGGAGCTAACAATGGCCTGCAGCAGGATGTGCTAGTCAGTCAGAAGGCTTGTGGCATGAAACGAGGCAACAAGAAGGAAGTGATAGATTTGAGGCGTCTTCTGATTTCATGTGCAGAAGCTGTTGCTGTTGATGATCGAGGGACTGCTAGTGAGCTACTAAAGCAAATTAGGCAGCACTCTTCCCCATTTGGTGATTATACTCAGAGGTTGGCTCATTGCTTTGCAAATGCTCTTGAAGCGCGGTTGGCTGGCACTGGAACTCAACTATATACTACTTGGATCCATACTACTTTAAGTACTAAACGAGTGTCAGGTGCAGATATTTCGAAGTTTTACCGAGCTTATATTGCAAGAGGAATAGATCCCATATCAGAAATGACTTCCACACTGGATGCTACATGGATGGAAGACATGGATGGAAGGGCAGACCTTAAGTCAGGTCAAGGGGGCGGGTTGGACATGTTTGAGAGGAATAGATCCCATATCAGAAATGACTTCCACACTGGATGCTACATGGATGGAAGGGCAGACCTTGCCCAACCCATGCTCAGGCGAGGCTGGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

376

Amino Acids

41.46

Weight (kDa)

5.46

Isoelectric Point (pI)

36.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 217 - 301 7.4e-16 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 802
AciI CCGC 5 cut(s) 206, 458, 527, 802, 1011
AclWI GGATC 5 cut(s) 552, 835, 848, 917, 1032
AcyI GRCGYC 1 cut(s) 655
AfaI GTAC 1 cut(s) 859
AfiI CCNNNNNNNGG 3 cut(s) 130, 583, 1110
AflII CTTAAG 1 cut(s) 992
AflIII ACRYGT 1 cut(s) 1020
AgsI TTSAA 3 cut(s) 56, 390, 797
AhdI GACNNNNNGTC 1 cut(s) 994
AluBI AGCT 7 cut(s) 190, 487, 519, 566, 680, 715, 902
AluI AGCT 7 cut(s) 190, 487, 519, 566, 680, 715, 902
Alw21I GWGCWC 1 cut(s) 104
Alw26I GTCTC 1 cut(s) 75
AlwI GGATC 5 cut(s) 552, 835, 848, 917, 1032
AlwNI CAGNNNCTG 1 cut(s) 680
AoxI GGCC 1 cut(s) 574
ApeKI GCWGC 2 cut(s) 580, 733
AspLEI GCGC 1 cut(s) 802
AsuHPI GGTGA 2 cut(s) 494, 764
AsuII TTCGAA 1 cut(s) 887
BamHI GGATCC 1 cut(s) 840
BbsI GAAGAC 2 cut(s) 650, 975
Bbv12I GWGCWC 1 cut(s) 104
BbvI GCAGC 2 cut(s) 592, 745
BccI CCATC 6 cut(s) 160, 248, 428, 958, 971, 1073
BceAI ACGGC 1 cut(s) 10
BcgI CGANNNNNNTGC 2 cut(s) 867, 901
BclI TGATCA 1 cut(s) 136
BcoDI GTCTC 1 cut(s) 75
BfaI CTAG 3 cut(s) 273, 593, 708
BfmI CTRYAG 2 cut(s) 291, 578
BfrI CTTAAG 1 cut(s) 992
BisI GCNGC 2 cut(s) 581, 734
BlpI GCTNAGC 1 cut(s) 515
BlsI GCNGC 2 cut(s) 582, 735
BmcAI AGTACT 1 cut(s) 859
BmeRI GACNNNNNGTC 1 cut(s) 994
BmiI GGNNCC 3 cut(s) 18, 198, 842
BmsI GCATC 3 cut(s) 193, 943, 1058
BpiI GAAGAC 2 cut(s) 650, 975
BplI GAGNNNNNCTC 2 cut(s) 757, 789
Bpu10I CCTNAGC 1 cut(s) 1109
Bpu1102I GCTNAGC 1 cut(s) 515
Bpu14I TTCGAA 1 cut(s) 887
BsaHI GRCGYC 1 cut(s) 655
BsaJI CCNNGG 1 cut(s) 241
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 583, 1110
Bse1I ACTGG 3 cut(s) 820, 954, 1069
Bse3DI GCAATG 1 cut(s) 775
BseDI CCNNGG 1 cut(s) 241
BseGI GGATG 9 cut(s) 259, 439, 475, 592, 958, 969, 982, 1073, 1084
BseLI CCNNNNNNNGG 3 cut(s) 130, 583, 1110
BseMI GCAATG 1 cut(s) 775
BseMII CTCAG 4 cut(s) 54, 529, 776, 1123
BseNI ACTGG 3 cut(s) 820, 954, 1069
BseRI GAGGAG 4 cut(s) 20, 80, 105, 431
BseXI GCAGC 2 cut(s) 592, 745
BsgI GTGCAG 2 cut(s) 693, 897
Bsh1236I CGCG 1 cut(s) 802
Bsh1285I CGRYCG 1 cut(s) 531
BshFI GGCC 1 cut(s) 576
BsiEI CGRYCG 1 cut(s) 531
BsiHKAI GWGCWC 1 cut(s) 104
BslFI GGGAC 1 cut(s) 715
BslI CCNNNNNNNGG 3 cut(s) 130, 583, 1110
BsmAI GTCTC 1 cut(s) 75
BsmFI GGGAC 1 cut(s) 715
BsnI GGCC 1 cut(s) 576
Bsp119I TTCGAA 1 cut(s) 887
Bsp1286I GDGCHC 1 cut(s) 104
Bsp143I GATC 6 cut(s) 136, 544, 694, 840, 922, 1037
Bsp1720I GCTNAGC 1 cut(s) 515
BspACI CCGC 5 cut(s) 206, 458, 527, 802, 1011
BspANI GGCC 1 cut(s) 576
BspCNI CTCAG 4 cut(s) 55, 528, 775, 1122
BspFNI CGCG 1 cut(s) 802
BspLI GGNNCC 3 cut(s) 18, 198, 842
BspMAI CTGCAG 2 cut(s) 295, 582
BspPI GGATC 5 cut(s) 552, 835, 848, 917, 1032
BspT104I TTCGAA 1 cut(s) 887
BspTI CTTAAG 1 cut(s) 992
BsrDI GCAATG 1 cut(s) 775
BsrI ACTGG 3 cut(s) 820, 954, 1069
BssECI CCNNGG 1 cut(s) 241
BssMI GATC 6 cut(s) 136, 544, 694, 840, 922, 1037
BssNI GRCGYC 1 cut(s) 655
Bst6I CTCTTC 1 cut(s) 745
BstACI GRCGYC 1 cut(s) 655
BstAFI CTTAAG 1 cut(s) 992
BstAPI GCANNNNNTGC 2 cut(s) 589, 1094
BstBI TTCGAA 1 cut(s) 887
BstC8I GCNNGC 2 cut(s) 578, 811
BstDEI CTNAG 4 cut(s) 63, 515, 762, 1109
BstENI CCTNNNNNAGG 1 cut(s) 581
BstF5I GGATG 9 cut(s) 259, 439, 475, 592, 958, 969, 982, 1073, 1084
BstFNI CGCG 1 cut(s) 802
BstHHI GCGC 1 cut(s) 802
BstKTI GATC 6 cut(s) 139, 547, 697, 843, 925, 1040
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 6 cut(s) 136, 544, 694, 840, 922, 1037
BstMCI CGRYCG 1 cut(s) 531
BstMWI GCNNNNNNNGC 7 cut(s) 589, 721, 730, 797, 806, 908, 1094
BstNSI RCATGY 3 cut(s) 34, 377, 1024
BstSFI CTRYAG 2 cut(s) 291, 578
BstUI CGCG 1 cut(s) 802
BstV1I GCAGC 2 cut(s) 592, 745
BstV2I GAAGAC 2 cut(s) 650, 975
BstX2I RGATCY 3 cut(s) 840, 922, 1037
BstXI CCANNNNNNTGG 1 cut(s) 362
BstYI RGATCY 3 cut(s) 840, 922, 1037
BsuRI GGCC 1 cut(s) 576
BtsCI GGATG 9 cut(s) 259, 439, 475, 592, 958, 969, 982, 1073, 1084
BtsI GCAGTG 1 cut(s) 373
BtsIMutI CAGTG 5 cut(s) 60, 373, 813, 947, 1062
Cac8I GCNNGC 2 cut(s) 578, 811
CaiI CAGNNNCTG 1 cut(s) 680
CfoI GCGC 1 cut(s) 802
CseI GACGC 1 cut(s) 644
Csp6I GTAC 1 cut(s) 858
CviQI GTAC 1 cut(s) 858
DdeI CTNAG 4 cut(s) 63, 515, 762, 1109
DpnI GATC 6 cut(s) 138, 546, 696, 842, 924, 1039
DpnII GATC 6 cut(s) 136, 544, 694, 840, 922, 1037
DriI GACNNNNNGTC 1 cut(s) 994
Eam1104I CTCTTC 1 cut(s) 745
Eam1105I GACNNNNNGTC 1 cut(s) 994
EarI CTCTTC 1 cut(s) 745
EcoNI CCTNNNNNAGG 1 cut(s) 581
FaqI GGGAC 1 cut(s) 715
FauI CCCGC 1 cut(s) 1004
FauNDI CATATG 1 cut(s) 310
FbaI TGATCA 1 cut(s) 136
Fnu4HI GCNGC 2 cut(s) 581, 734
FokI GGATG 9 cut(s) 266, 446, 482, 599, 965, 976, 989, 1080, 1091
Fsp4HI GCNGC 2 cut(s) 581, 734
FspBI CTAG 3 cut(s) 273, 593, 708
GlaI GCGC 1 cut(s) 801
GluI GCNGC 2 cut(s) 581, 734
HaeIII GGCC 1 cut(s) 576
HgaI GACGC 1 cut(s) 644
HhaI GCGC 1 cut(s) 802
Hin1I GRCGYC 1 cut(s) 655
Hin6I GCGC 1 cut(s) 800
HinP1I GCGC 1 cut(s) 800
HinfI GANTC 2 cut(s) 68, 403
HphI GGTGA 2 cut(s) 494, 764
Hpy188I TCNGA 8 cut(s) 411, 493, 558, 603, 663, 765, 934, 1049
Hpy188III TCNNGA 5 cut(s) 200, 390, 502, 532, 794
HpyAV CCTTC 5 cut(s) 499, 598, 628, 975, 1077
HpyCH4V TGCA 9 cut(s) 30, 232, 261, 293, 580, 674, 785, 878, 911
HpyF10VI GCNNNNNNNGC 7 cut(s) 589, 721, 730, 797, 806, 908, 1094
HpyF3I CTNAG 4 cut(s) 63, 515, 762, 1109
Hsp92I GRCGYC 1 cut(s) 655
HspAI GCGC 1 cut(s) 800
Ksp22I TGATCA 1 cut(s) 136
Kzo9I GATC 6 cut(s) 136, 544, 694, 840, 922, 1037
LmnI GCTCC 2 cut(s) 16, 563
Lsp1109I GCAGC 2 cut(s) 592, 745
LweI GCATC 3 cut(s) 193, 943, 1058
MaeI CTAG 3 cut(s) 273, 593, 708
MaeIII GTNAC 2 cut(s) 346, 536
MalI GATC 6 cut(s) 138, 546, 696, 842, 924, 1039
MboI GATC 6 cut(s) 136, 544, 694, 840, 922, 1037
MboII GAAGA 7 cut(s) 44, 260, 370, 451, 650, 732, 980
MflI RGATCY 3 cut(s) 840, 922, 1037
MhlI GDGCHC 1 cut(s) 104
MluCI AATT 3 cut(s) 221, 233, 726
MlyI GAGTC 2 cut(s) 77, 412
MmeI TCCRAC 1 cut(s) 996
MseI TTAA 3 cut(s) 444, 854, 993
MslI CAYNNNNRTG 1 cut(s) 378
MspA1I CMGCKG 1 cut(s) 519
MspCI CTTAAG 1 cut(s) 992
MvnI CGCG 1 cut(s) 802
MwoI GCNNNNNNNGC 7 cut(s) 589, 721, 730, 797, 806, 908, 1094
NdeI CATATG 1 cut(s) 310
NdeII GATC 6 cut(s) 136, 544, 694, 840, 922, 1037
NlaIV GGNNCC 3 cut(s) 18, 198, 842
NspI RCATGY 3 cut(s) 34, 377, 1024
NspV TTCGAA 1 cut(s) 887
PciI ACATGT 1 cut(s) 1020
PcsI WCGNNNNNNNCGW 1 cut(s) 537
PkrI GCNGC 2 cut(s) 582, 735
PleI GAGTC 2 cut(s) 76, 411
PpsI GAGTC 2 cut(s) 76, 411
PscI ACATGT 1 cut(s) 1020
PspN4I GGNNCC 3 cut(s) 18, 198, 842
PstI CTGCAG 2 cut(s) 295, 582
PstNI CAGNNNCTG 1 cut(s) 680
PsuI RGATCY 3 cut(s) 840, 922, 1037
PvuII CAGCTG 1 cut(s) 519
RsaI GTAC 1 cut(s) 859
RsaNI GTAC 1 cut(s) 858
RseI CAYNNNNRTG 1 cut(s) 378
SaqAI TTAA 3 cut(s) 444, 854, 993
SatI GCNGC 2 cut(s) 581, 734
Sau3AI GATC 6 cut(s) 136, 544, 694, 840, 922, 1037
ScaI AGTACT 1 cut(s) 859
SchI GAGTC 2 cut(s) 77, 412
SduI GDGCHC 1 cut(s) 104
SfaNI GCATC 3 cut(s) 193, 943, 1058
SfcI CTRYAG 2 cut(s) 291, 578
SfuI TTCGAA 1 cut(s) 887
SmiMI CAYNNNNRTG 1 cut(s) 378
SmlI CTYRAG 1 cut(s) 992
SmoI CTYRAG 1 cut(s) 992
Sse9I AATT 3 cut(s) 221, 233, 726
SsiI CCGC 5 cut(s) 206, 458, 527, 802, 1011
SspMI CTAG 3 cut(s) 273, 593, 708
TaqI TCGA 3 cut(s) 531, 697, 887
TaqII GACCGA 1 cut(s) 255
TasI AATT 3 cut(s) 221, 233, 726
TatI WGTACW 1 cut(s) 857
Tru1I TTAA 3 cut(s) 444, 854, 993
Tru9I TTAA 3 cut(s) 444, 854, 993
TscAI CASTG 5 cut(s) 67, 373, 820, 954, 1069
TseI GCWGC 2 cut(s) 580, 733
TspDTI ATGAA 3 cut(s) 368, 632, 657
TspRI CASTG 5 cut(s) 67, 373, 820, 954, 1069
Vha464I CTTAAG 1 cut(s) 992
XagI CCTNNNNNAGG 1 cut(s) 581
XceI RCATGY 3 cut(s) 34, 377, 1024
XspI CTAG 3 cut(s) 273, 593, 708
ZrmI AGTACT 1 cut(s) 859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.