Rh5DG457500

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000624
Physical Location & Seq
Forward (+)
72757446 .. 72760252
2807 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG457500.1

Sequence Viewer

Length: 1962 bp
ATGGATCCACGTTTCTCTGAATTTCCCAATTTTAATGATCAAACCCTTTTGCCTGATATGTTGAATGAGTACCCAAGTAGTCACCCTTTTGAAGATCTTAGCTTTTTGGACCCAAATCCCAGTAACTATGCTCTATCATCAAGCTTTGCCCCTGAGGGAGATGACGGTGAGTTTTCTGGTAGTGTGCTCAAGTACATAAACCAGGTGCTTATGGAGGAGGACATGGACCCTTTGGCTCTTCAAGCCACTGAGGAGTCTCTTTATGAGGCTATTGGAGGGAAGTACCCAACTTCCCATGACCAAAACCCTATTTGTACCGATCAAAATGTGCATGGAAATGGGAATGGGATGGTGGGTTGTGAGCTTATGGTTCCTGATGTAATTTGTAAGAATGAACTGTTAGTGCAATTTAACCGAGGGGCAGAGGAGGGTAGTAAGTTTCTTCCTAGAGGTCAATTGGTTAGTGCGGAGGGCAACAAGCCTTATGCTGTGGCGGATAGAAAGGCTGGAGATGCGGTAGTTATGACGCAGAAGAATGGCAGCGAGCATTTTTGTGTTAGTTCAAGAGGAAAGAAGAATCATCGGAGGGAGGAGATAGATTTGGAGGATGGGAGGTGTAACAAGCAATCGGCGGTTTATATGGATGATGAGGAGGGTGAGCTGTCTGATATTTTTGACAAGGTGTTGCTCTCCAAGCCTATCAAGAGTAAAGACGATCAAGTCAGTCAGAATGGAGCGAACAATGGCTTGCAGCAGGATGGGCAATCTGTTGGAATTGGTAATGGGAAGGTTTGTGGCAAGAATCAAGGCAACAAGAAGGAAGTGATAGATTTGAGGGCTCTTCTGATGTCATGTGCAGAAGCTGTATCTGTTGATGACTGCGAGACTGCTAGTGAGCTACTAAAGCAGATTATGCAGCACTCTTCCCCATTTGGTGATTATACTCAGAGGTTGGCTCATTGCTTTGCAAATGCTCTTGAAGCACGGTTGGCTGGCACCGGAACTCAAATATATACTGCTTTATCTACTAAACGAATGTCAGCTGCTGATACGCTGAAGTTTTACCAAGCTTACATTGCAGTCTGCCCGTTCATGAAGATGTTGATGGTCTTTGCAAACCATATGATTTTAAAAGCTGCTGAGAAAGCAGAAACACTACATATAATAGACTTTGGTATCCTTTATGGTTTTCAATGGCCTGCTCTCATTCATTGCCTCTCAAGAAGACCTGGAGGACCTCCTAAATTACGTATTACAGGGGTAGAGCTTCCCTTAAGTGGTTTTCGACCAGAAGAAAGAGTCCAAGAGACAGGGCATCGGCTTTCAAAATATTGTAAGCGATTTAATGTTCCTTTTGAGTACTGTGCCATAGCCAAAAAATGGGAAACTCTCCAATTTGATGACCTTAAAGTTCAAAGAAACGAGGTGCTTGCTGTTAATTGTCTGTGCCGATTTAAGTACCTCCTTGATGAGACGGTTGTGGTAAACAGTCCAAGGAATATTGTTCTAAATTTAATTAGGAAGTTGAATCCTGATGTCTTTGTTCAAAGTGTTGTTAATGGATCCCATCAGTTTCCCTTCTTTGTTCCACGCTTCCGAGAGGCACTATTCCACTTCTCTGCATTGTTTGATATGTTGGATAACAATTTTCCTCCTGACGATCAGATGAGATTGGGGTTTGAGAAAGAGTTTATTGGACGTGAAGTTCTGAATATAGTAGCTTGTGAGGGCTCTCAAAGAATTGTTAGGCCAGAAACATACAAGCAGTGGCAGATTCGAAACATGAGGGCTGGGTTCAGACAGCTGCCATTGGACCATGAAGTCATGAACAAAATAAAGGACAATGTAAAAATGGGGTACCACCCTTATTTTGTTGTTGATGAAGATGGCCACTGGATGCTGCAGGGATGGAAGGGCAGAATTATGTTTGCTTCTGCCTGTTGGGCACCATCTAGGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

653

Amino Acids

73.74

Weight (kDa)

5.68

Isoelectric Point (pI)

39.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 278 - 648 2.2e-117 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 719, 1820
Acc65I GGTACC 1 cut(s) 1857
AccB1I GGYRCC 3 cut(s) 995, 1857, 1945
AccB7I CCANNNNNTGG 1 cut(s) 1380
AciI CCGC 4 cut(s) 467, 494, 515, 632
AclWI GGATC 3 cut(s) 12, 1557, 1570
AcoI YGGCCR 1 cut(s) 1888
AcsI RAATTY 2 cut(s) 20, 1510
AcuI CTGAAG 1 cut(s) 1076
AfaI GTAC 7 cut(s) 71, 194, 284, 316, 1361, 1460, 1859
AfiI CCNNNNNNNGG 2 cut(s) 1277, 1380
AflII CTTAAG 1 cut(s) 1273
AjiI CACGTC 1 cut(s) 1700
AjnI CCWGG 2 cut(s) 201, 1228
AloI GAACNNNNNNTCC 2 cut(s) 1689, 1721
Alw21I GWGCWC 1 cut(s) 189
Alw26I GTCTC 4 cut(s) 261, 878, 1301, 1466
AlwI GGATC 3 cut(s) 12, 1557, 1570
AlwNI CAGNNNCTG 2 cut(s) 863, 1046
AoxI GGCC 3 cut(s) 1196, 1748, 1888
ApeKI GCWGC 7 cut(s) 540, 751, 916, 1043, 1136, 1804, 1900
ApoI RAATTY 2 cut(s) 20, 1510
Asp718I GGTACC 1 cut(s) 1857
AspS9I GGNCC 4 cut(s) 109, 226, 1235, 1813
AsuHPI GGTGA 4 cut(s) 74, 179, 668, 947
AsuII TTCGAA 1 cut(s) 1777
AvaII GGWCC 4 cut(s) 109, 226, 1235, 1813
AxyI CCTNAGG 1 cut(s) 153
BaeGI GKGCMC 1 cut(s) 1948
BaeI ACNNNNGTAYC 2 cut(s) 1159, 1192
BalI TGGCCA 1 cut(s) 1890
BamHI GGATCC 2 cut(s) 4, 1562
BanI GGYRCC 3 cut(s) 995, 1857, 1945
BanII GRGCYC 2 cut(s) 841, 1733
BbsI GAAGAC 1 cut(s) 1231
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 7 cut(s) 552, 763, 928, 1030, 1123, 1791, 1887
BccI CCATC 8 cut(s) 343, 602, 752, 1099, 1575, 1880, 1902, 1957
BcgI CGANNNNNNTGC 2 cut(s) 1412, 1446
BciT130I CCWGG 2 cut(s) 203, 1230
BciVI GTATCC 1 cut(s) 1187
BclI TGATCA 1 cut(s) 37
BcoDI GTCTC 4 cut(s) 261, 878, 1301, 1466
BfaI CTAG 3 cut(s) 447, 891, 1953
BfmI CTRYAG 1 cut(s) 1901
BfrI CTTAAG 1 cut(s) 1273
BfuI GTATCC 1 cut(s) 1187
BglI GCCNNNNNGGC 1 cut(s) 1943
BglII AGATCT 1 cut(s) 94
BisI GCNGC 7 cut(s) 541, 752, 917, 1044, 1137, 1805, 1901
BlsI GCNGC 7 cut(s) 542, 753, 918, 1045, 1138, 1806, 1902
BmcAI AGTACT 1 cut(s) 1361
Bme1390I CCNGG 2 cut(s) 203, 1230
Bme18I GGWCC 4 cut(s) 109, 226, 1235, 1813
BmgBI CACGTC 1 cut(s) 1700
BmgT120I GGNCC 4 cut(s) 109, 226, 1235, 1813
BmiI GGNNCC 8 cut(s) 6, 111, 228, 372, 997, 1564, 1859, 1947
BmrFI CCNGG 2 cut(s) 203, 1230
BmrI ACTGGG 1 cut(s) 114
BmsI GCATC 3 cut(s) 502, 1324, 1887
BmuI ACTGGG 1 cut(s) 114
BpiI GAAGAC 1 cut(s) 1231
BplI GAGNNNNNCTC 2 cut(s) 940, 972
BpmI CTGGAG 2 cut(s) 528, 1251
Bpu14I TTCGAA 1 cut(s) 1777
BpuEI CTTGAG 2 cut(s) 173, 1204
BsaAI YACGTR 1 cut(s) 1250
BsaJI CCNNGG 2 cut(s) 415, 1493
BsaWI WCCGGW 1 cut(s) 998
BsaXI ACNNNNNCTCC 2 cut(s) 501, 531
Bsc4I CCNNNNNNNGG 2 cut(s) 1277, 1380
Bse1I ACTGG 2 cut(s) 120, 1898
Bse21I CCTNAGG 1 cut(s) 153
Bse3DI GCAATG 3 cut(s) 958, 1074, 1210
BseBI CCWGG 2 cut(s) 203, 1230
BseDI CCNNGG 2 cut(s) 415, 1493
BseGI GGATG 6 cut(s) 354, 613, 649, 763, 1902, 1913
BseLI CCNNNNNNNGG 2 cut(s) 1277, 1380
BseMI GCAATG 3 cut(s) 958, 1074, 1210
BseMII CTCAG 4 cut(s) 144, 240, 959, 1131
BseNI ACTGG 2 cut(s) 120, 1898
BseRI GAGGAG 5 cut(s) 230, 266, 440, 605, 665
BseSI GKGCMC 1 cut(s) 1948
BseXI GCAGC 7 cut(s) 552, 763, 928, 1030, 1123, 1791, 1887
BseYI CCCAGC 1 cut(s) 1790
BsgI GTGCAG 1 cut(s) 876
BshFI GGCC 3 cut(s) 1198, 1750, 1890
BshNI GGYRCC 3 cut(s) 995, 1857, 1945
BsiHKAI GWGCWC 1 cut(s) 189
BsiSI CCGG 1 cut(s) 999
BslI CCNNNNNNNGG 2 cut(s) 1277, 1380
BsmAI GTCTC 4 cut(s) 261, 878, 1301, 1466
BsmBI CGTCTC 1 cut(s) 1466
BsnI GGCC 3 cut(s) 1198, 1750, 1890
Bsp119I TTCGAA 1 cut(s) 1777
Bsp1286I GDGCHC 4 cut(s) 189, 841, 1733, 1948
Bsp143I GATC 7 cut(s) 4, 37, 94, 319, 715, 1562, 1660
BspACI CCGC 4 cut(s) 467, 494, 515, 632
BspANI GGCC 3 cut(s) 1198, 1750, 1890
BspCNI CTCAG 4 cut(s) 145, 241, 958, 1132
BspHI TCATGA 2 cut(s) 1092, 1824
BspLI GGNNCC 8 cut(s) 6, 111, 228, 372, 997, 1564, 1859, 1947
BspMAI CTGCAG 1 cut(s) 1905
BspPI GGATC 3 cut(s) 12, 1557, 1570
BspQI GCTCTTC 2 cut(s) 243, 846
BspT104I TTCGAA 1 cut(s) 1777
BspT107I GGYRCC 3 cut(s) 995, 1857, 1945
BspTI CTTAAG 1 cut(s) 1273
BsrDI GCAATG 3 cut(s) 958, 1074, 1210
BsrI ACTGG 2 cut(s) 120, 1898
BssECI CCNNGG 2 cut(s) 415, 1493
BssMI GATC 7 cut(s) 4, 37, 94, 319, 715, 1562, 1660
BssT1I CCWWGG 1 cut(s) 1493
Bst2UI CCWGG 2 cut(s) 203, 1230
Bst4CI ACNGT 6 cut(s) 167, 399, 987, 1364, 1477, 1490
Bst6I CTCTTC 3 cut(s) 243, 846, 928
BstAFI CTTAAG 1 cut(s) 1273
BstAPI GCANNNNNTGC 1 cut(s) 913
BstBAI YACGTR 1 cut(s) 1250
BstBI TTCGAA 1 cut(s) 1777
BstC8I GCNNGC 5 cut(s) 545, 749, 994, 1200, 1431
BstDEI CTNAG 5 cut(s) 98, 153, 249, 945, 1140
BstF5I GGATG 6 cut(s) 354, 613, 649, 763, 1902, 1913
BstKTI GATC 7 cut(s) 7, 40, 97, 322, 718, 1565, 1663
BstMAI GTCTC 4 cut(s) 261, 878, 1301, 1466
BstMBI GATC 7 cut(s) 4, 37, 94, 319, 715, 1562, 1660
BstNI CCWGG 2 cut(s) 203, 1230
BstSCI CCNGG 2 cut(s) 201, 1228
BstSFI CTRYAG 1 cut(s) 1901
BstSLI GKGCMC 1 cut(s) 1948
BstSNI TACGTA 1 cut(s) 1250
BstV1I GCAGC 7 cut(s) 552, 763, 928, 1030, 1123, 1791, 1887
BstV2I GAAGAC 1 cut(s) 1231
BstX2I RGATCY 3 cut(s) 4, 94, 1562
BstYI RGATCY 3 cut(s) 4, 94, 1562
Bsu36I CCTNAGG 1 cut(s) 153
BsuI GTATCC 1 cut(s) 1187
BsuRI GGCC 3 cut(s) 1198, 1750, 1890
BtrI CACGTC 1 cut(s) 1700
BtsCI GGATG 6 cut(s) 354, 613, 649, 763, 1902, 1913
BtsI GCAGTG 1 cut(s) 1772
BtsIMutI CAGTG 3 cut(s) 246, 1772, 1891
Cac8I GCNNGC 5 cut(s) 545, 749, 994, 1200, 1431
CaiI CAGNNNCTG 2 cut(s) 863, 1046
CciI TCATGA 2 cut(s) 1092, 1824
Cfr13I GGNCC 4 cut(s) 109, 226, 1235, 1813
CseI GACGC 1 cut(s) 535
CsiI ACCWGGT 1 cut(s) 201
Csp6I GTAC 7 cut(s) 70, 193, 283, 315, 1360, 1459, 1858
CviAII CATG 8 cut(s) 223, 296, 332, 852, 1093, 1783, 1817, 1825
CviQI GTAC 7 cut(s) 70, 193, 283, 315, 1360, 1459, 1858
DdeI CTNAG 5 cut(s) 98, 153, 249, 945, 1140
DpnI GATC 7 cut(s) 6, 39, 96, 321, 717, 1564, 1662
DpnII GATC 7 cut(s) 4, 37, 94, 319, 715, 1562, 1660
DraI TTTAAA 1 cut(s) 1131
DrdI GACNNNNNNGTC 2 cut(s) 719, 1820
DseDI GACNNNNNNGTC 2 cut(s) 719, 1820
EaeI YGGCCR 1 cut(s) 1888
Eam1104I CTCTTC 3 cut(s) 243, 846, 928
EarI CTCTTC 3 cut(s) 243, 846, 928
EciI GGCGGA 1 cut(s) 509
Eco105I TACGTA 1 cut(s) 1250
Eco130I CCWWGG 1 cut(s) 1493
Eco24I GRGCYC 2 cut(s) 841, 1733
Eco47I GGWCC 4 cut(s) 109, 226, 1235, 1813
Eco57I CTGAAG 1 cut(s) 1076
Eco81I CCTNAGG 1 cut(s) 153
EcoO109I RGGNCCY 1 cut(s) 1235
EcoRII CCWGG 2 cut(s) 201, 1228
EcoT14I CCWWGG 1 cut(s) 1493
EcoT38I GRGCYC 2 cut(s) 841, 1733
ErhI CCWWGG 1 cut(s) 1493
Esp3I CGTCTC 1 cut(s) 1466
FaeI CATG 8 cut(s) 226, 299, 335, 855, 1096, 1786, 1820, 1828
FatI CATG 8 cut(s) 222, 295, 331, 851, 1092, 1782, 1816, 1824
FauNDI CATATG 1 cut(s) 1122
FbaI TGATCA 1 cut(s) 37
Fnu4HI GCNGC 7 cut(s) 541, 752, 917, 1044, 1137, 1805, 1901
FokI GGATG 6 cut(s) 361, 620, 656, 770, 1909, 1920
FriOI GRGCYC 2 cut(s) 841, 1733
Fsp4HI GCNGC 7 cut(s) 541, 752, 917, 1044, 1137, 1805, 1901
FspBI CTAG 3 cut(s) 447, 891, 1953
GluI GCNGC 7 cut(s) 541, 752, 917, 1044, 1137, 1805, 1901
GsaI CCCAGC 1 cut(s) 1794
GsuI CTGGAG 2 cut(s) 528, 1251
HaeIII GGCC 3 cut(s) 1198, 1750, 1890
HapII CCGG 1 cut(s) 999
HgaI GACGC 1 cut(s) 535
Hin1II CATG 8 cut(s) 226, 299, 335, 855, 1096, 1786, 1820, 1828
HindIII AAGCTT 2 cut(s) 142, 1068
HinfI GANTC 6 cut(s) 254, 577, 802, 1299, 1528, 1774
HpaII CCGG 1 cut(s) 999
HphI GGTGA 4 cut(s) 74, 179, 668, 947
Hpy166II GTNNAC 1 cut(s) 1486
Hpy188III TCNNGA 9 cut(s) 374, 564, 703, 977, 1093, 1221, 1532, 1655, 1825
Hpy8I GTNNAC 1 cut(s) 1486
HpyAV CCTTC 4 cut(s) 781, 811, 1588, 1906
HpyCH4III ACNGT 6 cut(s) 167, 399, 987, 1364, 1477, 1490
HpyCH4IV ACGT 3 cut(s) 10, 1249, 1699
HpyF3I CTNAG 5 cut(s) 98, 153, 249, 945, 1140
HpySE526I ACGT 3 cut(s) 10, 1249, 1699
Hsp92II CATG 8 cut(s) 226, 299, 335, 855, 1096, 1786, 1820, 1828
KpnI GGTACC 1 cut(s) 1861
Ksp22I TGATCA 1 cut(s) 37
Kzo9I GATC 7 cut(s) 4, 37, 94, 319, 715, 1562, 1660
LguI GCTCTTC 2 cut(s) 243, 846
LmnI GCTCC 1 cut(s) 734
Lsp1109I GCAGC 7 cut(s) 552, 763, 928, 1030, 1123, 1791, 1887
LweI GCATC 3 cut(s) 502, 1324, 1887
MabI ACCWGGT 1 cut(s) 201
MaeI CTAG 3 cut(s) 447, 891, 1953
MaeII ACGT 3 cut(s) 10, 1249, 1699
MaeIII GTNAC 3 cut(s) 80, 122, 617
MalI GATC 7 cut(s) 6, 39, 96, 321, 717, 1564, 1662
MboI GATC 7 cut(s) 4, 37, 94, 319, 715, 1562, 1660
MfeI CAATTG 1 cut(s) 455
MflI RGATCY 3 cut(s) 4, 94, 1562
MhlI GDGCHC 4 cut(s) 189, 841, 1733, 1948
MlsI TGGCCA 1 cut(s) 1890
MluNI TGGCCA 1 cut(s) 1890
MlyI GAGTC 2 cut(s) 263, 1308
MmeI TCCRAC 2 cut(s) 751, 1617
Mox20I TGGCCA 1 cut(s) 1890
MscI TGGCCA 1 cut(s) 1890
MslI CAYNNNNRTG 3 cut(s) 336, 552, 1097
Msp20I TGGCCA 1 cut(s) 1890
MspA1I CMGCKG 2 cut(s) 1043, 1804
MspCI CTTAAG 1 cut(s) 1273
MspI CCGG 1 cut(s) 999
MspR9I CCNGG 2 cut(s) 203, 1230
MunI CAATTG 1 cut(s) 455
MvaI CCWGG 2 cut(s) 203, 1230
NdeI CATATG 1 cut(s) 1122
NdeII GATC 7 cut(s) 4, 37, 94, 319, 715, 1562, 1660
NlaIII CATG 8 cut(s) 226, 299, 335, 855, 1096, 1786, 1820, 1828
NlaIV GGNNCC 8 cut(s) 6, 111, 228, 372, 997, 1564, 1859, 1947
NmuCI GTSAC 1 cut(s) 80
NspV TTCGAA 1 cut(s) 1777
PagI TCATGA 2 cut(s) 1092, 1824
PciSI GCTCTTC 2 cut(s) 243, 846
PfeI GAWTC 4 cut(s) 577, 802, 1528, 1774
PflMI CCANNNNNTGG 1 cut(s) 1380
PkrI GCNGC 7 cut(s) 542, 753, 918, 1045, 1138, 1806, 1902
PleI GAGTC 2 cut(s) 262, 1307
PpsI GAGTC 2 cut(s) 262, 1307
Ppu21I YACGTR 1 cut(s) 1250
PpuMI RGGWCCY 1 cut(s) 1235
Psp5II RGGWCCY 1 cut(s) 1235
Psp6I CCWGG 2 cut(s) 201, 1228
PspFI CCCAGC 1 cut(s) 1790
PspGI CCWGG 2 cut(s) 201, 1228
PspN4I GGNNCC 8 cut(s) 6, 111, 228, 372, 997, 1564, 1859, 1947
PspPI GGNCC 4 cut(s) 109, 226, 1235, 1813
PspPPI RGGWCCY 1 cut(s) 1235
PstI CTGCAG 1 cut(s) 1905
PstNI CAGNNNCTG 2 cut(s) 863, 1046
PsuI RGATCY 3 cut(s) 4, 94, 1562
PvuII CAGCTG 2 cut(s) 1043, 1804
RsaI GTAC 7 cut(s) 71, 194, 284, 316, 1361, 1460, 1859
RsaNI GTAC 7 cut(s) 70, 193, 283, 315, 1360, 1459, 1858
RseI CAYNNNNRTG 3 cut(s) 336, 552, 1097
SapI GCTCTTC 2 cut(s) 243, 846
SatI GCNGC 7 cut(s) 541, 752, 917, 1044, 1137, 1805, 1901
Sau3AI GATC 7 cut(s) 4, 37, 94, 319, 715, 1562, 1660
Sau96I GGNCC 4 cut(s) 109, 226, 1235, 1813
ScaI AGTACT 1 cut(s) 1361
SchI GAGTC 2 cut(s) 263, 1308
ScrFI CCNGG 2 cut(s) 203, 1230
SduI GDGCHC 4 cut(s) 189, 841, 1733, 1948
SexAI ACCWGGT 1 cut(s) 201
SfaNI GCATC 3 cut(s) 502, 1324, 1887
SfcI CTRYAG 1 cut(s) 1901
SfuI TTCGAA 1 cut(s) 1777
SinI GGWCC 4 cut(s) 109, 226, 1235, 1813
SmiMI CAYNNNNRTG 3 cut(s) 336, 552, 1097
SmlI CTYRAG 3 cut(s) 188, 1219, 1273
SmoI CTYRAG 3 cut(s) 188, 1219, 1273
SnaBI TACGTA 1 cut(s) 1250
SsiI CCGC 4 cut(s) 467, 494, 515, 632
SspI AATATT 2 cut(s) 1331, 1501
SspMI CTAG 3 cut(s) 447, 891, 1953
StyD4I CCNGG 2 cut(s) 201, 1228
StyI CCWWGG 1 cut(s) 1493
TaaI ACNGT 6 cut(s) 167, 399, 987, 1364, 1477, 1490
TaiI ACGT 3 cut(s) 13, 1252, 1702
TaqI TCGA 2 cut(s) 1285, 1777
TatI WGTACW 2 cut(s) 192, 1359
TfiI GAWTC 4 cut(s) 577, 802, 1528, 1774
TscAI CASTG 3 cut(s) 253, 1772, 1898
TseFI GTSAC 1 cut(s) 80
TseI GCWGC 7 cut(s) 540, 751, 916, 1043, 1136, 1804, 1900
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 7 cut(s) 408, 1081, 1109, 1199, 1833, 1841, 1896
TspRI CASTG 3 cut(s) 253, 1772, 1898
Van91I CCANNNNNTGG 1 cut(s) 1380
Vha464I CTTAAG 1 cut(s) 1273
VpaK11BI GGWCC 4 cut(s) 109, 226, 1235, 1813
XapI RAATTY 2 cut(s) 20, 1510
XspI CTAG 3 cut(s) 447, 891, 1953
ZrmI AGTACT 1 cut(s) 1361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.