FvH4_3g36580

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000624
Physical Location & Seq
Forward (+)
31321542 .. 31327658
6117 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g36580.t1

Sequence Viewer

Length: 1650 bp
ATGTTGAACGAGTACCCAAGTGTTGACCTTTTTGATGATCTTAGCTTTTTGGACCCAAACCCCATGAACTCTGCTCTGTCTTCGAGCTTTAGCCCTGTAGGAGATGATGGTGAGTTTTCTAACACTGTGATCAAGCACATAAATCAGGTGCTTATGGAGGAGGACATGGAGCCAACACCATGTCTGTTCCCTGATCCTTTGGTTCTTCAAGCCACTGAGGAGTCTCTTTATGAGATTATTGGAGCAGGGAAGTGTTCAACTTCCCTAGACCAAAACCCTCTTTGGGTTGATCAAAATGAGTATGGAAATGGAAATGAGATGGTGGGTTCTTATAGGAGTGAGCTTATGGTTCGTGATGCGGTTACGGAGGGCAACAAGCCGTATACTGTGGCGAATAGCAAGGCAGGAAATGTGGTTGTTAGGACCCAGAAGAATGGAAGTGAGCATGTCCGTGTTGGTTCAAGAGGAAATAATAGTCATCTGAGGGAGGAGGTAGAGTTGGAGGATGGGAGGAATGACAAGCAATCTGTGGTTTATGTGGATGATGAGGAGGGTGAGCTGTCTGATAGTTTTGGCAAGGCGTTGCTCGGCGGTAAGGCTGTTGAAAGTAAAGAGGATCAAGTCTGTCAAAATGGAGCTAACAATGGCCTGCAGCAGGATGTGCTAGTCAGTCAGAAGGCTTGTGGCATGAAACGAGGCAACAAGAAGGAAGTGATAGATTTGAGGGGTCTTCTGATTTCATGTGCAGAAGCTGTTGCTGTTGATGATCGAGGGACTGCTAGTGAGCTACTAAAGCAAATTAGGCAGCACTCTTCCCCATTTGGTGATTATACTCAGAGGTTGTCTCATTGCTTTGCAAATGCTCTTGAAGCGTGGTTTCAATGGCCTGCTCTCATTCATGGCCTCTCAAGAAGACCTGGTGGACCTCCCAAATTACGCATTACAGGGGTAGAGCTTACCGTAAGTGGTTGTCAACCAGAAGAAACAGTCCAAGAGACAGGGCATCGCTTGGCAAATTATTGTAAGCGATTTAATGTTCCTTTTGAGTACTATGCCATAGCCAAGAAATGGGAAACACTCCAACTTGTTGACCTTAAAGTTCAAAGAAATGAGGTGCTTGCTGTTAATTGTCTCTGCCGATTTAAGTACCTCCTTGATGAGACAGTTATGGTAAACAGTCCAAGGGATACTGTTCTAAATTTAATTAGAAAGTTGAATCCTGATATATTTGTCCAAAGTGTTGTCAATGGATCCCATGATGCACCCTTCTTTGTTCCACGCTTCCGAGAGGCACTATTCCACTTCTCTGCATACTTTGATTTGTTGGATACTAATTTGCCTCGCGAAGATCAGATAAGATTGGGGTTTGAAAAAGAGTTTATTGGACGTGAAGTTCTGAATATAGTTGCTTGTGAGGGCACTAAAAGAATTGCGAGGCCTGAAACATATAAGCAGTGGCAGATTCGAAACACGAGGGCTGGTTTCAGACAGCTGCCATTGGACTATGAAGTTGTGAACAAAATAAAGGATAAGGTAAAAATGGGATACCACCCGTTTTTTGTGGTTAATGAAGATGGCCACTGGATGCTGCAGGGTTGGAAGGGTAGAATTTTGGTTGGTTCAGCCTGTTGGGCACCTTCTAGGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

550

Amino Acids

61.85

Weight (kDa)

5.43

Isoelectric Point (pI)

39.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 242 - 291 9.8e-10 GRAS domain family
GRAS PF03514 293 - 544 5e-75 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1633
AccB7I CCANNNNNTGG 1 cut(s) 1068
AccI GTMKAC 1 cut(s) 383
AccII CGCG 1 cut(s) 1344
AciI CCGC 2 cut(s) 359, 591
AclWI GGATC 4 cut(s) 188, 624, 1245, 1258
AcoI YGGCCR 1 cut(s) 1576
AcsI RAATTY 2 cut(s) 1198, 1608
AfaI GTAC 3 cut(s) 14, 1049, 1148
AfiI CCNNNNNNNGG 3 cut(s) 283, 655, 1068
AjiI CACGTC 1 cut(s) 1388
AjnI CCWGG 1 cut(s) 916
AloI GAACNNNNNNTCC 2 cut(s) 1377, 1409
AluBI AGCT 9 cut(s) 45, 87, 343, 559, 638, 752, 787, 955, 1492
AluI AGCT 9 cut(s) 45, 87, 343, 559, 638, 752, 787, 955, 1492
Alw26I GTCTC 5 cut(s) 228, 849, 989, 1136, 1154
AlwI GGATC 4 cut(s) 188, 624, 1245, 1258
AlwNI CAGNNNCTG 1 cut(s) 752
AoxI GGCC 5 cut(s) 646, 884, 901, 1436, 1576
ApeKI GCWGC 4 cut(s) 652, 805, 1492, 1588
ApoI RAATTY 2 cut(s) 1198, 1608
AspS9I GGNCC 3 cut(s) 52, 423, 923
AsuHPI GGTGA 3 cut(s) 122, 566, 836
AsuII TTCGAA 1 cut(s) 1465
AvaII GGWCC 3 cut(s) 52, 423, 923
BaeGI GKGCMC 2 cut(s) 1421, 1636
BalI TGGCCA 1 cut(s) 1578
BamHI GGATCC 1 cut(s) 1250
BanI GGYRCC 1 cut(s) 1633
BauI CACGAG 1 cut(s) 1471
BbsI GAAGAC 3 cut(s) 72, 722, 919
BbvI GCAGC 4 cut(s) 664, 817, 1479, 1575
BccI CCATC 4 cut(s) 101, 313, 500, 1568
BceAI ACGGC 1 cut(s) 364
BciT130I CCWGG 1 cut(s) 918
BciVI GTATCC 3 cut(s) 1180, 1321, 1538
BclI TGATCA 2 cut(s) 129, 289
BcoDI GTCTC 5 cut(s) 228, 849, 989, 1136, 1154
BfaI CTAG 4 cut(s) 266, 665, 780, 1641
BfmI CTRYAG 3 cut(s) 96, 650, 1589
BfuI GTATCC 3 cut(s) 1180, 1321, 1538
BglI GCCNNNNNGGC 1 cut(s) 1631
BisI GCNGC 4 cut(s) 653, 806, 1493, 1589
BlsI GCNGC 4 cut(s) 654, 807, 1494, 1590
BmcAI AGTACT 1 cut(s) 1049
Bme1390I CCNGG 1 cut(s) 918
Bme18I GGWCC 3 cut(s) 52, 423, 923
BmgBI CACGTC 1 cut(s) 1388
BmgT120I GGNCC 3 cut(s) 52, 423, 923
BmiI GGNNCC 5 cut(s) 54, 171, 425, 1252, 1635
BmrFI CCNGG 1 cut(s) 918
BmsI GCATC 4 cut(s) 346, 1012, 1249, 1575
BpiI GAAGAC 3 cut(s) 72, 722, 919
BplI GAGNNNNNCTC 2 cut(s) 829, 861
Bpu14I TTCGAA 1 cut(s) 1465
BpuEI CTTGAG 1 cut(s) 892
BsaJI CCNNGG 1 cut(s) 1181
BsaXI ACNNNNNCTCC 2 cut(s) 482, 512
Bsc4I CCNNNNNNNGG 3 cut(s) 283, 655, 1068
Bse1I ACTGG 1 cut(s) 1586
Bse3DI GCAATG 1 cut(s) 847
BseBI CCWGG 1 cut(s) 918
BseDI CCNNGG 1 cut(s) 1181
BseGI GGATG 4 cut(s) 511, 547, 664, 1590
BseLI CCNNNNNNNGG 3 cut(s) 283, 655, 1068
BseMI GCAATG 1 cut(s) 847
BseMII CTCAG 3 cut(s) 207, 473, 848
BseNI ACTGG 1 cut(s) 1586
BseRI GAGGAG 4 cut(s) 173, 233, 503, 563
BseSI GKGCMC 2 cut(s) 1421, 1636
BseXI GCAGC 4 cut(s) 664, 817, 1479, 1575
BsgI GTGCAG 1 cut(s) 765
Bsh1236I CGCG 1 cut(s) 1344
BshFI GGCC 5 cut(s) 648, 886, 903, 1438, 1578
BshNI GGYRCC 1 cut(s) 1633
BslFI GGGAC 1 cut(s) 787
BslI CCNNNNNNNGG 3 cut(s) 283, 655, 1068
BsmAI GTCTC 5 cut(s) 228, 849, 989, 1136, 1154
BsmFI GGGAC 1 cut(s) 787
BsnI GGCC 5 cut(s) 648, 886, 903, 1438, 1578
Bsp119I TTCGAA 1 cut(s) 1465
Bsp1286I GDGCHC 2 cut(s) 1421, 1636
Bsp143I GATC 8 cut(s) 37, 129, 193, 289, 616, 766, 1250, 1348
Bsp68I TCGCGA 1 cut(s) 1344
BspACI CCGC 2 cut(s) 359, 591
BspANI GGCC 5 cut(s) 648, 886, 903, 1438, 1578
BspCNI CTCAG 3 cut(s) 208, 474, 847
BspFNI CGCG 1 cut(s) 1344
BspLI GGNNCC 5 cut(s) 54, 171, 425, 1252, 1635
BspMAI CTGCAG 2 cut(s) 654, 1593
BspPI GGATC 4 cut(s) 188, 624, 1245, 1258
BspT104I TTCGAA 1 cut(s) 1465
BspT107I GGYRCC 1 cut(s) 1633
BsrDI GCAATG 1 cut(s) 847
BsrI ACTGG 1 cut(s) 1586
BssECI CCNNGG 1 cut(s) 1181
BssMI GATC 8 cut(s) 37, 129, 193, 289, 616, 766, 1250, 1348
BssNAI GTATAC 1 cut(s) 384
BssSI CACGAG 1 cut(s) 1471
BssT1I CCWWGG 1 cut(s) 1181
Bst1107I GTATAC 1 cut(s) 384
Bst2BI CACGAG 1 cut(s) 1471
Bst2UI CCWGG 1 cut(s) 918
Bst4CI ACNGT 7 cut(s) 127, 388, 961, 988, 1165, 1178, 1192
Bst6I CTCTTC 1 cut(s) 817
BstAPI GCANNNNNTGC 1 cut(s) 661
BstBI TTCGAA 1 cut(s) 1465
BstC8I GCNNGC 3 cut(s) 650, 888, 1119
BstDEI CTNAG 4 cut(s) 41, 216, 482, 834
BstENI CCTNNNNNAGG 1 cut(s) 653
BstF5I GGATG 4 cut(s) 511, 547, 664, 1590
BstFNI CGCG 1 cut(s) 1344
BstKTI GATC 8 cut(s) 40, 132, 196, 292, 619, 769, 1253, 1351
BstMAI GTCTC 5 cut(s) 228, 849, 989, 1136, 1154
BstMBI GATC 8 cut(s) 37, 129, 193, 289, 616, 766, 1250, 1348
BstMWI GCNNNNNNNGC 5 cut(s) 661, 793, 802, 869, 1631
BstNI CCWGG 1 cut(s) 918
BstNSI RCATGY 1 cut(s) 449
BstSCI CCNGG 1 cut(s) 916
BstSFI CTRYAG 3 cut(s) 96, 650, 1589
BstSLI GKGCMC 2 cut(s) 1421, 1636
BstUI CGCG 1 cut(s) 1344
BstV1I GCAGC 4 cut(s) 664, 817, 1479, 1575
BstV2I GAAGAC 3 cut(s) 72, 722, 919
BstX2I RGATCY 1 cut(s) 1250
BstXI CCANNNNNNTGG 1 cut(s) 434
BstYI RGATCY 1 cut(s) 1250
BstZ17I GTATAC 1 cut(s) 384
BsuI GTATCC 3 cut(s) 1180, 1321, 1538
BsuRI GGCC 5 cut(s) 648, 886, 903, 1438, 1578
BtgZI GCGATG 1 cut(s) 989
BtrI CACGTC 1 cut(s) 1388
BtsCI GGATG 4 cut(s) 511, 547, 664, 1590
BtsI GCAGTG 1 cut(s) 1460
BtsIMutI CAGTG 4 cut(s) 123, 213, 1460, 1579
BtuMI TCGCGA 1 cut(s) 1344
Cac8I GCNNGC 3 cut(s) 650, 888, 1119
CaiI CAGNNNCTG 1 cut(s) 752
Cfr13I GGNCC 3 cut(s) 52, 423, 923
CsiI ACCWGGT 1 cut(s) 916
Csp6I GTAC 3 cut(s) 13, 1048, 1147
CviAII CATG 8 cut(s) 64, 166, 180, 446, 688, 741, 899, 1256
CviQI GTAC 3 cut(s) 13, 1048, 1147
DdeI CTNAG 4 cut(s) 41, 216, 482, 834
DpnI GATC 8 cut(s) 39, 131, 195, 291, 618, 768, 1252, 1350
DpnII GATC 8 cut(s) 37, 129, 193, 289, 616, 766, 1250, 1348
EaeI YGGCCR 1 cut(s) 1576
Eam1104I CTCTTC 1 cut(s) 817
EarI CTCTTC 1 cut(s) 817
Eco130I CCWWGG 1 cut(s) 1181
Eco147I AGGCCT 1 cut(s) 1438
Eco47I GGWCC 3 cut(s) 52, 423, 923
EcoNI CCTNNNNNAGG 1 cut(s) 653
EcoO109I RGGNCCY 1 cut(s) 423
EcoRII CCWGG 1 cut(s) 916
EcoT14I CCWWGG 1 cut(s) 1181
ErhI CCWWGG 1 cut(s) 1181
FaeI CATG 8 cut(s) 67, 169, 183, 449, 691, 744, 902, 1259
FaqI GGGAC 1 cut(s) 787
FatI CATG 8 cut(s) 63, 165, 179, 445, 687, 740, 898, 1255
FbaI TGATCA 2 cut(s) 129, 289
FblI GTMKAC 1 cut(s) 383
Fnu4HI GCNGC 4 cut(s) 653, 806, 1493, 1589
FokI GGATG 4 cut(s) 518, 554, 671, 1597
Fsp4HI GCNGC 4 cut(s) 653, 806, 1493, 1589
FspBI CTAG 4 cut(s) 266, 665, 780, 1641
GluI GCNGC 4 cut(s) 653, 806, 1493, 1589
HaeIII GGCC 5 cut(s) 648, 886, 903, 1438, 1578
Hin1II CATG 8 cut(s) 67, 169, 183, 449, 691, 744, 902, 1259
HincII GTYRAC 3 cut(s) 25, 974, 1090
HindII GTYRAC 3 cut(s) 25, 974, 1090
HinfI GANTC 3 cut(s) 221, 1216, 1462
HphI GGTGA 3 cut(s) 122, 566, 836
Hpy166II GTNNAC 7 cut(s) 25, 384, 923, 974, 1090, 1174, 1516
Hpy188I TCNGA 9 cut(s) 483, 565, 675, 735, 837, 1286, 1353, 1398, 1487
Hpy188III TCNNGA 6 cut(s) 353, 462, 866, 909, 1220, 1343
Hpy8I GTNNAC 7 cut(s) 25, 384, 923, 974, 1090, 1174, 1516
HpyAV CCTTC 5 cut(s) 670, 700, 1276, 1594, 1647
HpyCH4III ACNGT 7 cut(s) 127, 388, 961, 988, 1165, 1178, 1192
HpyCH4IV ACGT 1 cut(s) 1387
HpyCH4V TGCA 6 cut(s) 652, 746, 857, 1262, 1310, 1591
HpyF10VI GCNNNNNNNGC 5 cut(s) 661, 793, 802, 869, 1631
HpyF3I CTNAG 4 cut(s) 41, 216, 482, 834
HpySE526I ACGT 1 cut(s) 1387
Hsp92II CATG 8 cut(s) 67, 169, 183, 449, 691, 744, 902, 1259
Ksp22I TGATCA 2 cut(s) 129, 289
Kzo9I GATC 8 cut(s) 37, 129, 193, 289, 616, 766, 1250, 1348
LmnI GCTCC 3 cut(s) 169, 242, 635
Lsp1109I GCAGC 4 cut(s) 664, 817, 1479, 1575
LweI GCATC 4 cut(s) 346, 1012, 1249, 1575
MabI ACCWGGT 1 cut(s) 916
MaeI CTAG 4 cut(s) 266, 665, 780, 1641
MaeII ACGT 1 cut(s) 1387
MaeIII GTNAC 1 cut(s) 361
MalI GATC 8 cut(s) 39, 131, 195, 291, 618, 768, 1252, 1350
MboI GATC 8 cut(s) 37, 129, 193, 289, 616, 766, 1250, 1348
MboII GAAGA 9 cut(s) 72, 197, 442, 722, 804, 924, 992, 1358, 1583
MflI RGATCY 1 cut(s) 1250
MhlI GDGCHC 2 cut(s) 1421, 1636
MlsI TGGCCA 1 cut(s) 1578
MluCI AATT 9 cut(s) 798, 932, 1015, 1126, 1198, 1203, 1333, 1428, 1608
MluNI TGGCCA 1 cut(s) 1578
MlyI GAGTC 1 cut(s) 230
MmeI TCCRAC 4 cut(s) 480, 1105, 1305, 1577
Mox20I TGGCCA 1 cut(s) 1578
MscI TGGCCA 1 cut(s) 1578
MseI TTAA 6 cut(s) 1032, 1095, 1125, 1143, 1202, 1566
MslI CAYNNNNRTG 1 cut(s) 450
Msp20I TGGCCA 1 cut(s) 1578
MspA1I CMGCKG 1 cut(s) 1492
MspR9I CCNGG 1 cut(s) 918
MvaI CCWGG 1 cut(s) 918
MvnI CGCG 1 cut(s) 1344
MwoI GCNNNNNNNGC 5 cut(s) 661, 793, 802, 869, 1631
NdeII GATC 8 cut(s) 37, 129, 193, 289, 616, 766, 1250, 1348
NlaIII CATG 8 cut(s) 67, 169, 183, 449, 691, 744, 902, 1259
NlaIV GGNNCC 5 cut(s) 54, 171, 425, 1252, 1635
NmeAIII GCCGAG 1 cut(s) 567
NruI TCGCGA 1 cut(s) 1344
NspI RCATGY 1 cut(s) 449
NspV TTCGAA 1 cut(s) 1465
PceI AGGCCT 1 cut(s) 1438
PfeI GAWTC 2 cut(s) 1216, 1462
PflMI CCANNNNNTGG 1 cut(s) 1068
PkrI GCNGC 4 cut(s) 654, 807, 1494, 1590
PleI GAGTC 1 cut(s) 229
PpsI GAGTC 1 cut(s) 229
PpuMI RGGWCCY 1 cut(s) 423
Psp5II RGGWCCY 1 cut(s) 423
Psp6I CCWGG 1 cut(s) 916
PspGI CCWGG 1 cut(s) 916
PspN4I GGNNCC 5 cut(s) 54, 171, 425, 1252, 1635
PspPI GGNCC 3 cut(s) 52, 423, 923
PspPPI RGGWCCY 1 cut(s) 423
PstI CTGCAG 2 cut(s) 654, 1593
PstNI CAGNNNCTG 1 cut(s) 752
PsuI RGATCY 1 cut(s) 1250
PvuII CAGCTG 1 cut(s) 1492
RruI TCGCGA 1 cut(s) 1344
RsaI GTAC 3 cut(s) 14, 1049, 1148
RsaNI GTAC 3 cut(s) 13, 1048, 1147
RseI CAYNNNNRTG 1 cut(s) 450
SaqAI TTAA 6 cut(s) 1032, 1095, 1125, 1143, 1202, 1566
SatI GCNGC 4 cut(s) 653, 806, 1493, 1589
Sau3AI GATC 8 cut(s) 37, 129, 193, 289, 616, 766, 1250, 1348
Sau96I GGNCC 3 cut(s) 52, 423, 923
ScaI AGTACT 1 cut(s) 1049
SchI GAGTC 1 cut(s) 230
ScrFI CCNGG 1 cut(s) 918
SduI GDGCHC 2 cut(s) 1421, 1636
SexAI ACCWGGT 1 cut(s) 916
SfaNI GCATC 4 cut(s) 346, 1012, 1249, 1575
SfcI CTRYAG 3 cut(s) 96, 650, 1589
SfuI TTCGAA 1 cut(s) 1465
SinI GGWCC 3 cut(s) 52, 423, 923
SmiMI CAYNNNNRTG 1 cut(s) 450
SmlI CTYRAG 1 cut(s) 907
SmoI CTYRAG 1 cut(s) 907
Sse9I AATT 9 cut(s) 798, 932, 1015, 1126, 1198, 1203, 1333, 1428, 1608
SseBI AGGCCT 1 cut(s) 1438
SsiI CCGC 2 cut(s) 359, 591
SspMI CTAG 4 cut(s) 266, 665, 780, 1641
StuI AGGCCT 1 cut(s) 1438
StyD4I CCNGG 1 cut(s) 916
StyI CCWWGG 1 cut(s) 1181
TaaI ACNGT 7 cut(s) 127, 388, 961, 988, 1165, 1178, 1192
TaiI ACGT 1 cut(s) 1390
TaqI TCGA 3 cut(s) 83, 769, 1465
TasI AATT 9 cut(s) 798, 932, 1015, 1126, 1198, 1203, 1333, 1428, 1608
TatI WGTACW 1 cut(s) 1047
TfiI GAWTC 2 cut(s) 1216, 1462
Tru1I TTAA 6 cut(s) 1032, 1095, 1125, 1143, 1202, 1566
Tru9I TTAA 6 cut(s) 1032, 1095, 1125, 1143, 1202, 1566
TscAI CASTG 4 cut(s) 130, 220, 1460, 1586
TseI GCWGC 4 cut(s) 652, 805, 1492, 1588
TspDTI ATGAA 6 cut(s) 80, 704, 729, 887, 1521, 1584
TspGWI ACGGA 2 cut(s) 380, 440
TspRI CASTG 4 cut(s) 130, 220, 1460, 1586
Van91I CCANNNNNTGG 1 cut(s) 1068
VpaK11BI GGWCC 3 cut(s) 52, 423, 923
XagI CCTNNNNNAGG 1 cut(s) 653
XapI RAATTY 2 cut(s) 1198, 1608
XceI RCATGY 1 cut(s) 449
XmiI GTMKAC 1 cut(s) 383
XspI CTAG 4 cut(s) 266, 665, 780, 1641
ZrmI AGTACT 1 cut(s) 1049
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.