Rroxscaffold_1G00015650

oxidosqualene cyclase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000624
Physical Location & Seq
Reverse (-)
19446371 .. 19449456
3086 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00015650.1

Sequence Viewer

Length: 2190 bp
ATGGATCCACGTTTCTCTGAATTTCCCAATTTCAATGATCAAACCCTTTTGCCCGATATGTTGAATGAGTACCCAAGTAGTGACCCTTTCGAAGATCTTAGCTTTTCGGACCCAAACCCCAGTAACTCTGCTCTGTCGTCGAGCTTTAGCGCTGATGGAGATGATGTTGAGTTTTCTGATAGTGTGCTCAAGTACATAAACCAGGTCCTTATGGAGGAGGACATGGAGTCAAAGCCATGTATGTTCCATGATCCTTTGGCTCTTGAAGCCACTGAGGAGTCTCTTTATGAGGTTATTGGGGGAGGGAAGTACCCAATTTCCCAAGACCAAAACCGTCTTTGTGTTGATCAAAATGTTCAGAGCCCAGATGGTGGTTATTCTTCTGGAAGTTTCAGTGATTCCAGCTCTAGTCCTGGTCCTTCCGTTGACTCTTGCCCAATCACTGCCCAGTTTGTAGAGACTAGGAGCTCTATATTGCAAATCCCTATTCCTGAAAACTTTGTTTTCCAGTCCCCTTCCAGGGCTAGGTCTCAATCGTCGTCGGATGGGTATGGAAATGGGAATGGGATGGTGGGTTCTTATAGGAGTGAGCTAATGGTTCCTGATGTGCTTAGTAAGGATGAATTGTTAGTGCAGTTTAACCTAGGGGCAGAGGAGGGTAGTAAGTTTCTTCCTAGAGGTCAATTGATTAGTGCGGAGGGCAACAAGATGTATGCTGTGGTGAATAGAAAGGCTGGAGATGTGGTAGTAACAACGCAGAAGAATGGGAGTGAGCAATTTCATGTTGGTTCAAGAGGAAAAAAGAATCATCAAAGGGAAGATATAGATTTGGAGGATGGGAGGAGTAACAAGCAACCAGCAGTTTATATGGACGATGAGGAGGGTGAGCTGTCTGATATTTTTGACAAGGTGTTGCTCTGTAAGCCTTTGGAGAGTAAAGACGATGAAGCCAGTCAGAATGGAGCAAACAATGGCCTGCAGCAGGATGTGCAATCTGTTGGAATTGCTAATGGGAAGGGTCGCGGCAAGATTCGTGGCAACAAGAAGGAAGTCATAGATTTGAGGGCTCTTCTGATTTTATGTGCACAAGCTGTTTCTGTTGATGATCGTGAGACTGCTAGTGAGCTACTAAAGCAGATTATGCAGCACTCTTCCCCATTTGGTGATTGTGCTCAGAGGTTGGCTCATTGCTTTGCAAATGCTCTTGAAGCGCGGTTAGCTGGCACTGGAACTCAAATATATACTGCTTTATCTACTAAACGAATGTCAGCTGCTGATATGCTGAAGTTTTACCAAGCTTATATTGCAGTCTGCCCATTCATGAAGATGTCCATGATCTTTGCAAACCATATGATTTTAAAAGCAGCTGAGAAAGCAGAAACACTTCATATAATAGATTTTGGTATCCTTTATGGTTTTCAGTGGCCTGCTCTTATTCATTGCCTTTCAAGAAGACCTGGTGGACCTCCCAAATTACGTATTACAGGGGTAGAGCTTCCCTTAAGTGGTTTTCGACCAGAAGAAAGAGTCCAAGAGACAGGGCATCGCTTGGCAAAGTATTGTAAGCGATTTAATGTTCCTTTTGAGTACTTTGCCATAGCCAAAAAATGGGAAACTGTCCAATTTGATGACCTCAAAGTTCAAAGAAATGAGGTGCTTGCTGTTAATTGTCTCTGCCGATTTAAGAACCTCCTTGATGAGACAGTTGTGGTAAACAGTCCAAGGGATACTGTTCTAAATTTAATTAGGAAGTTAAATCCTGATGTCTTTGTCCAAAGTGTTGTTAATGGATCCTATCATGCGCCCTTCTTTGTTTCACGCTTCCGAGAGGCGCTATTCCACTTCTCTGCATTGTTTGATATGTTTGATAACAATTTGCCTCGTGAAGATCAGATGAGATTAGGGTTTGAGAAAGAGTTTATTGGACGTGAAGTTGTGAATATAGTAGCTTGTGAGGGCTCAGAAAGAATTGTGAGGCCAGAAACATATAAGCAGTGGCAGGTTCGGAACTTGAGGGCTGGGTTCAGACAGCTGCCCTTGGACCGTGAAGTCATGATCAAAATAAAGGATAAAGTAAAAACGGGGTACAGCCCTGATTTTGTGGTTGATGAGGATGGCCGCTGGATGCTGCAGGGATGGAAGGGCAGAATTATATTTTGTTCCGCCTGTTGGGCACCGCCTAGGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 map00100 map00909 map01100 map01110
Pfam Domains
Protein Families
Prenyltrans SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

729

Amino Acids

81.75

Weight (kDa)

5.3

Isoelectric Point (pI)

45.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 354 - 724 6.9e-118 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2048
Acc36I ACCTGC 1 cut(s) 1990
AccB1I GGYRCC 1 cut(s) 2173
AccB7I CCANNNNNTGG 2 cut(s) 371, 1608
AccII CGCG 2 cut(s) 1023, 1213
AciI CCGC 6 cut(s) 695, 1023, 1213, 2119, 2163, 2177
AclWI GGATC 4 cut(s) 12, 245, 1785, 1798
AcoI YGGCCR 1 cut(s) 2116
AcsI RAATTY 2 cut(s) 20, 1738
AcuI CTGAAG 1 cut(s) 1304
AfaI GTAC 5 cut(s) 71, 194, 311, 1589, 2087
AfeI AGCGCT 1 cut(s) 151
AfiI CCNNNNNNNGG 9 cut(s) 214, 371, 519, 520, 525, 982, 1505, 1608, 2169
AflII CTTAAG 1 cut(s) 1501
AgsI TTSAA 7 cut(s) 34, 64, 266, 792, 1208, 1449, 1643
AhdI GACNNNNNGTC 1 cut(s) 226
AjiI CACGTC 1 cut(s) 1928
AjnI CCWGG 4 cut(s) 201, 412, 518, 1456
AloI GAACNNNNNNTCC 2 cut(s) 559, 591
Alw21I GWGCWC 4 cut(s) 189, 470, 1087, 1174
Alw26I GTCTC 7 cut(s) 285, 452, 534, 1106, 1529, 1676, 1694
Alw44I GTGCAC 1 cut(s) 1083
AlwI GGATC 4 cut(s) 12, 245, 1785, 1798
AlwNI CAGNNNCTG 1 cut(s) 1274
Aor51HI AGCGCT 1 cut(s) 151
AoxI GGCC 4 cut(s) 973, 1424, 1976, 2116
ApaLI GTGCAC 1 cut(s) 1083
ApeKI GCWGC 6 cut(s) 979, 1144, 1271, 1364, 2032, 2128
ApoI RAATTY 2 cut(s) 20, 1738
Asp700I GAANNNNTTC 1 cut(s) 1383
AspA2I CCTAGG 2 cut(s) 643, 2180
AspLEI GCGC 4 cut(s) 152, 1213, 1804, 1834
AspS9I GGNCC 5 cut(s) 109, 205, 416, 1463, 2041
AsuHPI GGTGA 3 cut(s) 733, 896, 1175
AsuII TTCGAA 1 cut(s) 90
AvaII GGWCC 5 cut(s) 109, 205, 416, 1463, 2041
AvrII CCTAGG 2 cut(s) 643, 2180
BaeGI GKGCMC 2 cut(s) 1087, 2176
BamHI GGATCC 2 cut(s) 4, 1790
BanI GGYRCC 1 cut(s) 2173
BanII GRGCYC 4 cut(s) 365, 470, 1069, 1961
BauI CACGAG 1 cut(s) 1881
BbsI GAAGAC 1 cut(s) 1459
Bbv12I GWGCWC 4 cut(s) 189, 470, 1087, 1174
BbvI GCAGC 6 cut(s) 991, 1156, 1258, 1376, 2019, 2115
BccI CCATC 7 cut(s) 149, 362, 539, 562, 830, 2108, 2130
BciT130I CCWGG 4 cut(s) 203, 414, 520, 1458
BciVI GTATCC 2 cut(s) 1415, 1720
BclI TGATCA 3 cut(s) 37, 346, 2055
BcoDI GTCTC 7 cut(s) 285, 452, 534, 1106, 1529, 1676, 1694
BfaI CTAG 7 cut(s) 408, 462, 525, 644, 675, 1119, 2181
BfmI CTRYAG 2 cut(s) 977, 2129
BfoI RGCGCY 2 cut(s) 153, 1835
BfrI CTTAAG 1 cut(s) 1501
BfuAI ACCTGC 1 cut(s) 1990
BfuI GTATCC 2 cut(s) 1415, 1720
BglI GCCNNNNNGGC 1 cut(s) 2171
BglII AGATCT 1 cut(s) 94
BisI GCNGC 8 cut(s) 980, 1024, 1145, 1272, 1365, 2033, 2119, 2129
BlnI CCTAGG 2 cut(s) 643, 2180
BlsI GCNGC 8 cut(s) 981, 1025, 1146, 1273, 1366, 2034, 2120, 2130
BmcAI AGTACT 1 cut(s) 1589
Bme1390I CCNGG 4 cut(s) 203, 414, 520, 1458
Bme18I GGWCC 5 cut(s) 109, 205, 416, 1463, 2041
BmeRI GACNNNNNGTC 1 cut(s) 226
BmgBI CACGTC 1 cut(s) 1928
BmgT120I GGNCC 5 cut(s) 109, 205, 416, 1463, 2041
BmiI GGNNCC 5 cut(s) 6, 111, 600, 1792, 2175
BmrFI CCNGG 4 cut(s) 203, 414, 520, 1458
BmrI ACTGGG 2 cut(s) 114, 442
BmsI GCATC 2 cut(s) 1552, 2115
BmuI ACTGGG 2 cut(s) 114, 442
BpiI GAAGAC 1 cut(s) 1459
BplI GAGNNNNNCTC 2 cut(s) 1168, 1200
BpmI CTGGAG 1 cut(s) 756
Bpu14I TTCGAA 1 cut(s) 90
BpuEI CTTGAG 2 cut(s) 173, 2032
BsaAI YACGTR 1 cut(s) 1478
BsaI GGTCTC 1 cut(s) 534
BsaJI CCNNGG 5 cut(s) 519, 643, 1721, 2037, 2180
BsaXI ACNNNNNCTCC 4 cut(s) 150, 180, 729, 759
Bsc4I CCNNNNNNNGG 9 cut(s) 214, 371, 519, 520, 525, 982, 1505, 1608, 2169
Bse1I ACTGG 5 cut(s) 120, 448, 508, 951, 1231
Bse3DI GCAATG 2 cut(s) 1186, 1438
BseBI CCWGG 4 cut(s) 203, 414, 520, 1458
BseDI CCNNGG 5 cut(s) 519, 643, 1721, 2037, 2180
BseGI GGATG 8 cut(s) 550, 573, 625, 841, 991, 2119, 2130, 2141
BseLI CCNNNNNNNGG 9 cut(s) 214, 371, 519, 520, 525, 982, 1505, 1608, 2169
BseMI GCAATG 2 cut(s) 1186, 1438
BseMII CTCAG 4 cut(s) 264, 1187, 1359, 1974
BseNI ACTGG 5 cut(s) 120, 448, 508, 951, 1231
BseRI GAGGAG 5 cut(s) 230, 290, 668, 856, 893
BseSI GKGCMC 2 cut(s) 1087, 2176
BseXI GCAGC 6 cut(s) 991, 1156, 1258, 1376, 2019, 2115
BseYI CCCAGC 1 cut(s) 2018
BsgI GTGCAG 1 cut(s) 653
Bsh1236I CGCG 2 cut(s) 1023, 1213
BshFI GGCC 4 cut(s) 975, 1426, 1978, 2118
BshNI GGYRCC 1 cut(s) 2173
BsiHKAI GWGCWC 4 cut(s) 189, 470, 1087, 1174
BslFI GGGAC 1 cut(s) 496
BslI CCNNNNNNNGG 9 cut(s) 214, 371, 519, 520, 525, 982, 1505, 1608, 2169
BsmAI GTCTC 7 cut(s) 285, 452, 534, 1106, 1529, 1676, 1694
BsmFI GGGAC 1 cut(s) 496
BsnI GGCC 4 cut(s) 975, 1426, 1978, 2118
Bso31I GGTCTC 1 cut(s) 534
Bsp119I TTCGAA 1 cut(s) 90
Bsp1286I GDGCHC 8 cut(s) 189, 365, 470, 1069, 1087, 1174, 1961, 2176
BspACI CCGC 6 cut(s) 695, 1023, 1213, 2119, 2163, 2177
BspANI GGCC 4 cut(s) 975, 1426, 1978, 2118
BspCNI CTCAG 4 cut(s) 265, 1186, 1360, 1973
BspFNI CGCG 2 cut(s) 1023, 1213
BspHI TCATGA 2 cut(s) 1320, 2052
BspLI GGNNCC 5 cut(s) 6, 111, 600, 1792, 2175
BspMAI CTGCAG 2 cut(s) 981, 2133
BspMI ACCTGC 1 cut(s) 1990
BspPI GGATC 4 cut(s) 12, 245, 1785, 1798
BspQI GCTCTTC 1 cut(s) 1074
BspT104I TTCGAA 1 cut(s) 90
BspT107I GGYRCC 1 cut(s) 2173
BspTI CTTAAG 1 cut(s) 1501
BspTNI GGTCTC 1 cut(s) 534
BsrDI GCAATG 2 cut(s) 1186, 1438
BsrI ACTGG 5 cut(s) 120, 448, 508, 951, 1231
BssECI CCNNGG 5 cut(s) 519, 643, 1721, 2037, 2180
BssSI CACGAG 1 cut(s) 1881
BssT1I CCWWGG 4 cut(s) 643, 1721, 2037, 2180
Bst2BI CACGAG 1 cut(s) 1881
Bst2UI CCWGG 4 cut(s) 203, 414, 520, 1458
Bst4CI ACNGT 6 cut(s) 335, 1618, 1705, 1718, 1732, 2045
Bst6I CTCTTC 2 cut(s) 1074, 1156
BstAFI CTTAAG 1 cut(s) 1501
BstAPI GCANNNNNTGC 2 cut(s) 988, 1141
BstBAI YACGTR 1 cut(s) 1478
BstBI TTCGAA 1 cut(s) 90
BstC8I GCNNGC 4 cut(s) 977, 1222, 1428, 1659
BstDEI CTNAG 6 cut(s) 98, 273, 611, 1173, 1368, 1960
BstENI CCTNNNNNAGG 2 cut(s) 212, 980
BstF5I GGATG 8 cut(s) 550, 573, 625, 841, 991, 2119, 2130, 2141
BstFNI CGCG 2 cut(s) 1023, 1213
BstH2I RGCGCY 2 cut(s) 153, 1835
BstHHI GCGC 4 cut(s) 152, 1213, 1804, 1834
BstMAI GTCTC 7 cut(s) 285, 452, 534, 1106, 1529, 1676, 1694
BstNI CCWGG 4 cut(s) 203, 414, 520, 1458
BstSCI CCNGG 4 cut(s) 201, 412, 518, 1456
BstSFI CTRYAG 2 cut(s) 977, 2129
BstSLI GKGCMC 2 cut(s) 1087, 2176
BstSNI TACGTA 1 cut(s) 1478
BstUI CGCG 2 cut(s) 1023, 1213
BstV1I GCAGC 6 cut(s) 991, 1156, 1258, 1376, 2019, 2115
BstV2I GAAGAC 1 cut(s) 1459
BstX2I RGATCY 3 cut(s) 4, 94, 1790
BstYI RGATCY 3 cut(s) 4, 94, 1790
BsuI GTATCC 2 cut(s) 1415, 1720
BsuRI GGCC 4 cut(s) 975, 1426, 1978, 2118
BtgZI GCGATG 1 cut(s) 1529
BtrI CACGTC 1 cut(s) 1928
BtsCI GGATG 8 cut(s) 550, 573, 625, 841, 991, 2119, 2130, 2141
BtsI GCAGTG 2 cut(s) 441, 2000
BtsIMutI CAGTG 6 cut(s) 270, 400, 441, 1224, 1427, 2000
BveI ACCTGC 1 cut(s) 1990
Cac8I GCNNGC 4 cut(s) 977, 1222, 1428, 1659
CaiI CAGNNNCTG 1 cut(s) 1274
CciI TCATGA 2 cut(s) 1320, 2052
CfoI GCGC 4 cut(s) 152, 1213, 1804, 1834
Cfr13I GGNCC 5 cut(s) 109, 205, 416, 1463, 2041
CsiI ACCWGGT 2 cut(s) 201, 1456
Csp6I GTAC 5 cut(s) 70, 193, 310, 1588, 2086
CspCI CAANNNNNGTGG 2 cut(s) 1015, 1050
CviAII CATG 8 cut(s) 223, 237, 248, 782, 1321, 1333, 1799, 2053
CviQI GTAC 5 cut(s) 70, 193, 310, 1588, 2086
DdeI CTNAG 6 cut(s) 98, 273, 611, 1173, 1368, 1960
DraI TTTAAA 1 cut(s) 1359
DrdI GACNNNNNNGTC 1 cut(s) 2048
DriI GACNNNNNGTC 1 cut(s) 226
DseDI GACNNNNNNGTC 1 cut(s) 2048
EaeI YGGCCR 1 cut(s) 2116
Eam1104I CTCTTC 2 cut(s) 1074, 1156
Eam1105I GACNNNNNGTC 1 cut(s) 226
EarI CTCTTC 2 cut(s) 1074, 1156
EciI GGCGGA 1 cut(s) 2152
Ecl136II GAGCTC 1 cut(s) 468
Eco105I TACGTA 1 cut(s) 1478
Eco130I CCWWGG 4 cut(s) 643, 1721, 2037, 2180
Eco24I GRGCYC 4 cut(s) 365, 470, 1069, 1961
Eco31I GGTCTC 1 cut(s) 534
Eco47I GGWCC 5 cut(s) 109, 205, 416, 1463, 2041
Eco47III AGCGCT 1 cut(s) 151
Eco53kI GAGCTC 1 cut(s) 468
Eco57I CTGAAG 1 cut(s) 1304
EcoICRI GAGCTC 1 cut(s) 468
EcoNI CCTNNNNNAGG 2 cut(s) 212, 980
EcoO109I RGGNCCY 1 cut(s) 205
EcoRII CCWGG 4 cut(s) 201, 412, 518, 1456
EcoT14I CCWWGG 4 cut(s) 643, 1721, 2037, 2180
EcoT38I GRGCYC 4 cut(s) 365, 470, 1069, 1961
ErhI CCWWGG 4 cut(s) 643, 1721, 2037, 2180
FaeI CATG 8 cut(s) 226, 240, 251, 785, 1324, 1336, 1802, 2056
FaqI GGGAC 1 cut(s) 496
FatI CATG 8 cut(s) 222, 236, 247, 781, 1320, 1332, 1798, 2052
FauNDI CATATG 1 cut(s) 1350
FbaI TGATCA 3 cut(s) 37, 346, 2055
Fnu4HI GCNGC 8 cut(s) 980, 1024, 1145, 1272, 1365, 2033, 2119, 2129
FokI GGATG 8 cut(s) 557, 580, 632, 848, 998, 2126, 2137, 2148
FriOI GRGCYC 4 cut(s) 365, 470, 1069, 1961
Fsp4HI GCNGC 8 cut(s) 980, 1024, 1145, 1272, 1365, 2033, 2119, 2129
FspBI CTAG 7 cut(s) 408, 462, 525, 644, 675, 1119, 2181
GlaI GCGC 4 cut(s) 151, 1212, 1803, 1833
GluI GCNGC 8 cut(s) 980, 1024, 1145, 1272, 1365, 2033, 2119, 2129
GsaI CCCAGC 1 cut(s) 2022
GsuI CTGGAG 1 cut(s) 756
HaeII RGCGCY 2 cut(s) 153, 1835
HaeIII GGCC 4 cut(s) 975, 1426, 1978, 2118
HhaI GCGC 4 cut(s) 152, 1213, 1804, 1834
Hin1II CATG 8 cut(s) 226, 240, 251, 785, 1324, 1336, 1802, 2056
Hin6I GCGC 4 cut(s) 150, 1211, 1802, 1832
HinP1I GCGC 4 cut(s) 150, 1211, 1802, 1832
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HindIII AAGCTT 1 cut(s) 1296
HinfI GANTC 7 cut(s) 227, 278, 398, 428, 805, 1030, 1527
HphI GGTGA 3 cut(s) 733, 896, 1175
Hpy166II GTNNAC 4 cut(s) 427, 1085, 1463, 1714
Hpy8I GTNNAC 4 cut(s) 427, 1085, 1463, 1714
Hpy99I CGWCG 3 cut(s) 142, 541, 544
HpyAV CCTTC 6 cut(s) 429, 525, 1009, 1039, 1816, 2134
HpyCH4III ACNGT 6 cut(s) 335, 1618, 1705, 1718, 1732, 2045
HpyCH4IV ACGT 3 cut(s) 10, 1477, 1927
HpyF3I CTNAG 6 cut(s) 98, 273, 611, 1173, 1368, 1960
HpySE526I ACGT 3 cut(s) 10, 1477, 1927
Hsp92II CATG 8 cut(s) 226, 240, 251, 785, 1324, 1336, 1802, 2056
HspAI GCGC 4 cut(s) 150, 1211, 1802, 1832
Ksp22I TGATCA 3 cut(s) 37, 346, 2055
LguI GCTCTTC 1 cut(s) 1074
LmnI GCTCC 2 cut(s) 465, 962
Lsp1109I GCAGC 6 cut(s) 991, 1156, 1258, 1376, 2019, 2115
LweI GCATC 2 cut(s) 1552, 2115
MabI ACCWGGT 2 cut(s) 201, 1456
MaeI CTAG 7 cut(s) 408, 462, 525, 644, 675, 1119, 2181
MaeII ACGT 3 cut(s) 10, 1477, 1927
MaeIII GTNAC 4 cut(s) 80, 122, 748, 845
MfeI CAATTG 1 cut(s) 683
MflI RGATCY 3 cut(s) 4, 94, 1790
MhlI GDGCHC 8 cut(s) 189, 365, 470, 1069, 1087, 1174, 1961, 2176
MlyI GAGTC 4 cut(s) 236, 287, 422, 1536
MmeI TCCRAC 2 cut(s) 522, 979
MroXI GAANNNNTTC 1 cut(s) 1383
MseI TTAA 9 cut(s) 639, 1358, 1502, 1572, 1665, 1683, 1742, 1754, 1785
MslI CAYNNNNRTG 1 cut(s) 1325
MspA1I CMGCKG 4 cut(s) 1271, 1367, 2032, 2121
MspCI CTTAAG 1 cut(s) 1501
MspR9I CCNGG 4 cut(s) 203, 414, 520, 1458
MunI CAATTG 1 cut(s) 683
MvaI CCWGG 4 cut(s) 203, 414, 520, 1458
MvnI CGCG 2 cut(s) 1023, 1213
NdeI CATATG 1 cut(s) 1350
NlaIII CATG 8 cut(s) 226, 240, 251, 785, 1324, 1336, 1802, 2056
NlaIV GGNNCC 5 cut(s) 6, 111, 600, 1792, 2175
NmuCI GTSAC 1 cut(s) 80
NspV TTCGAA 1 cut(s) 90
PagI TCATGA 2 cut(s) 1320, 2052
PciSI GCTCTTC 1 cut(s) 1074
PdmI GAANNNNTTC 1 cut(s) 1383
PfeI GAWTC 3 cut(s) 398, 805, 1030
PflMI CCANNNNNTGG 2 cut(s) 371, 1608
PkrI GCNGC 8 cut(s) 981, 1025, 1146, 1273, 1366, 2034, 2120, 2130
PleI GAGTC 4 cut(s) 235, 286, 422, 1535
PpsI GAGTC 4 cut(s) 235, 286, 422, 1535
Ppu21I YACGTR 1 cut(s) 1478
PpuMI RGGWCCY 1 cut(s) 205
Psp124BI GAGCTC 1 cut(s) 470
Psp5II RGGWCCY 1 cut(s) 205
Psp6I CCWGG 4 cut(s) 201, 412, 518, 1456
PspFI CCCAGC 1 cut(s) 2018
PspGI CCWGG 4 cut(s) 201, 412, 518, 1456
PspN4I GGNNCC 5 cut(s) 6, 111, 600, 1792, 2175
PspPI GGNCC 5 cut(s) 109, 205, 416, 1463, 2041
PspPPI RGGWCCY 1 cut(s) 205
PstI CTGCAG 2 cut(s) 981, 2133
PstNI CAGNNNCTG 1 cut(s) 1274
PsuI RGATCY 3 cut(s) 4, 94, 1790
PvuII CAGCTG 3 cut(s) 1271, 1367, 2032
RsaI GTAC 5 cut(s) 71, 194, 311, 1589, 2087
RsaNI GTAC 5 cut(s) 70, 193, 310, 1588, 2086
RseI CAYNNNNRTG 1 cut(s) 1325
SacI GAGCTC 1 cut(s) 470
SapI GCTCTTC 1 cut(s) 1074
SaqAI TTAA 9 cut(s) 639, 1358, 1502, 1572, 1665, 1683, 1742, 1754, 1785
SatI GCNGC 8 cut(s) 980, 1024, 1145, 1272, 1365, 2033, 2119, 2129
Sau96I GGNCC 5 cut(s) 109, 205, 416, 1463, 2041
ScaI AGTACT 1 cut(s) 1589
SchI GAGTC 4 cut(s) 236, 287, 422, 1536
ScrFI CCNGG 4 cut(s) 203, 414, 520, 1458
SduI GDGCHC 8 cut(s) 189, 365, 470, 1069, 1087, 1174, 1961, 2176
SexAI ACCWGGT 2 cut(s) 201, 1456
SfaNI GCATC 2 cut(s) 1552, 2115
SfcI CTRYAG 2 cut(s) 977, 2129
SfuI TTCGAA 1 cut(s) 90
SinI GGWCC 5 cut(s) 109, 205, 416, 1463, 2041
SmiMI CAYNNNNRTG 1 cut(s) 1325
SmlI CTYRAG 3 cut(s) 188, 1501, 2011
SmoI CTYRAG 3 cut(s) 188, 1501, 2011
SnaBI TACGTA 1 cut(s) 1478
SsiI CCGC 6 cut(s) 695, 1023, 1213, 2119, 2163, 2177
SspMI CTAG 7 cut(s) 408, 462, 525, 644, 675, 1119, 2181
SstI GAGCTC 1 cut(s) 470
StyD4I CCNGG 4 cut(s) 201, 412, 518, 1456
StyI CCWWGG 4 cut(s) 643, 1721, 2037, 2180
TaaI ACNGT 6 cut(s) 335, 1618, 1705, 1718, 1732, 2045
TaiI ACGT 3 cut(s) 13, 1480, 1930
TaqI TCGA 3 cut(s) 90, 140, 1513
TatI WGTACW 2 cut(s) 192, 1587
TauI GCSGC 2 cut(s) 1026, 2121
TfiI GAWTC 3 cut(s) 398, 805, 1030
Tru1I TTAA 9 cut(s) 639, 1358, 1502, 1572, 1665, 1683, 1742, 1754, 1785
Tru9I TTAA 9 cut(s) 639, 1358, 1502, 1572, 1665, 1683, 1742, 1754, 1785
TscAI CASTG 6 cut(s) 277, 400, 448, 1231, 1427, 2000
TseFI GTSAC 1 cut(s) 80
TseI GCWGC 6 cut(s) 979, 1144, 1271, 1364, 2032, 2128
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 7 cut(s) 636, 770, 960, 1309, 1337, 1376, 1427
TspGWI ACGGA 1 cut(s) 412
TspRI CASTG 6 cut(s) 277, 400, 448, 1231, 1427, 2000
Van91I CCANNNNNTGG 2 cut(s) 371, 1608
Vha464I CTTAAG 1 cut(s) 1501
VneI GTGCAC 1 cut(s) 1083
VpaK11BI GGWCC 5 cut(s) 109, 205, 416, 1463, 2041
XagI CCTNNNNNAGG 2 cut(s) 212, 980
XapI RAATTY 2 cut(s) 20, 1738
XmaJI CCTAGG 2 cut(s) 643, 2180
XmnI GAANNNNTTC 1 cut(s) 1383
XspI CTAG 7 cut(s) 408, 462, 525, 644, 675, 1119, 2181
ZrmI AGTACT 1 cut(s) 1589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.