FvH4_3g36641

oxidosqualene cyclase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000624
Physical Location & Seq
Forward (+)
31387744 .. 31389312
1569 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g36641.t1

Sequence Viewer

Length: 1569 bp
ATGGTCAATGCTCCGTTTGTAGAAACCAGGAGCTCTATATTGCAAAACCCAATTCCTGAAAACTTTGTTTTCCAGTCCCCTTCCAGGGCTAACAGTCAGTCATCAACAAATAGGTATGGAGAAGGGAATGGGAATTGGATGGTGGGTTCTTATAGAAGGGAGTTTTTGGTTCCTGATGTGGTTAGTAAGAATGAATTGTTATTGCAATTTAACCGAGGGGCAGAGGAGGGTAGTAAGTTTCTTCCTAGAGGTCAGTTGATTAGTGCGGAGGGCAACAAGACATATGTGGTGGCAAATAGCAAGGCAGGAAATATGGTTGTTACGATGCAGAATGGAAGTGAGCATGTTCGTGTTGGGTCAAGAGGAAAAAAGCATCATCAAAGGGAAGATACAGATTTGGAGGATGGGAGGAGTAACAAGCAGTCGGCTGTTTATATGGACGATGAGGAGGGTGAGCTGTCAGATATTTTTGACAAGGTGTTGCTGTGTAAGCCTTTGGAGAGTAGAGACAATCAAGTCAGTCAGAATGGAGCAGACAACGGGCAATCTGTTGGAATTGGTAATGGGATGGTTCGTGGCAAGAGTCAAGGCAACAAGAAGAAAGTTGTAGATTTGTGGGCTCTTCTAATTTTATGTGCACAAGCTGTTTCTGTTGATGATCTGGAGACTGCTAGTGAGCTACTACAGCAGATTACGCAGCACTTTTCCCCATCTGGTGATTGCACTCAGAGGTTGGCTCATTGCTTTGCAAATGCTCTTGAAGCGCGGTTGGCTGGCACTGGAACTCAAATATATACTGCTTTATCTTCTAAACGAAGGTCAGCTGCTGATATGCTGAGGTTTTACCAAACTTATATTGCAGCCTGCCCTTTCATGAAGATGTCGTTAATCTTCGCAAACCATATGATTTTAAAAGCGGCTGAAAAAGCAGACACACTTCATATAATAGATTTTGGCATCCTTTATGGTTTTCAATGGCCTGCTCTCATTCATTGCCTCTCAAGAAGACCTGGTGGACCTCCCAAATTACGCATTACAGGGGTAGAGCTTCCCTTAAGTGGTTTTCGACCAGAAGAAAGAGTCCAAGAGACAGGGCATCGCTTGGCAAAGTATTGTAAGCGATTTAATGTTCCTTTCGAGTACTATGCCATAGCCAAAAAATGGGAAACTGTCCAATTTGATGACCTCAAAGTTCAAAGAAATGAGGTGGTTGCTGTTAATTGTGTGTGCCGATTTAAGGATCTCCTTGATGAGACAGTTGTGGTAACCAGTCCAAGGGATACTGTTCTAAATTTAATCAGGAAGTTGAATCCTGATGTCTTTGTCCAAACTGTTGTAAATGGATCCTATCATGCACCCTTCTTTGTTTCACGCTTCCGAGAGGCAATATTCCACTTCTCTGCGTTGTTTGATATGTTTGATTCCAATTTGCCTCGTGATGATCAGATGAGATTGGGGTTTGAGAAAGAGTTTATTGGACGTGAAGTTGTGAATATAGTAGCATGTGAGGGCACTGAAAGAACTGTGAGGCCAGAAAAACATATAAGCAGTGGCAGGTACGGAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 map00100 map00909 map01100 map01110
Pfam Domains
Protein Families
Prenyltrans SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

523

Amino Acids

58.64

Weight (kDa)

8.23

Isoelectric Point (pI)

41.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 207 - 513 9.8e-101 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 515
Acc36I ACCTGC 1 cut(s) 1545
AccB7I CCANNNNNTGG 1 cut(s) 1161
AccII CGCG 1 cut(s) 766
AciI CCGC 3 cut(s) 266, 766, 917
AclWI GGATC 3 cut(s) 1248, 1338, 1351
AcsI RAATTY 1 cut(s) 1291
AfaI GTAC 2 cut(s) 1142, 1559
AfiI CCNNNNNNNGG 6 cut(s) 84, 85, 1058, 1161, 1237, 1275
AflII CTTAAG 1 cut(s) 1054
AgsI TTSAA 4 cut(s) 761, 974, 1196, 1309
AjiI CACGTC 1 cut(s) 1481
AjnI CCWGG 3 cut(s) 26, 83, 1009
AloI GAACNNNNNNTCC 2 cut(s) 130, 162
AluBI AGCT 6 cut(s) 33, 457, 644, 679, 824, 1048
AluI AGCT 6 cut(s) 33, 457, 644, 679, 824, 1048
Alw21I GWGCWC 2 cut(s) 35, 640
Alw26I GTCTC 4 cut(s) 501, 659, 1082, 1247
Alw44I GTGCAC 1 cut(s) 636
AlwI GGATC 3 cut(s) 1248, 1338, 1351
AlwNI CAGNNNCTG 1 cut(s) 827
AoxI GGCC 2 cut(s) 977, 1529
ApaLI GTGCAC 1 cut(s) 636
ApeKI GCWGC 3 cut(s) 697, 824, 860
ApoI RAATTY 1 cut(s) 1291
AspLEI GCGC 1 cut(s) 766
AspS9I GGNCC 1 cut(s) 1016
AsuHPI GGTGA 2 cut(s) 464, 728
AvaII GGWCC 1 cut(s) 1016
BaeGI GKGCMC 2 cut(s) 640, 1514
BamHI GGATCC 1 cut(s) 1343
BanII GRGCYC 2 cut(s) 35, 622
BauI CACGAG 1 cut(s) 1434
BbsI GAAGAC 1 cut(s) 1012
Bbv12I GWGCWC 2 cut(s) 35, 640
BbvCI CCTCAGC 1 cut(s) 836
BbvI GCAGC 3 cut(s) 709, 811, 872
BccI CCATC 4 cut(s) 133, 398, 562, 718
BcgI CGANNNNNNTGC 2 cut(s) 1127, 1161
BciT130I CCWGG 3 cut(s) 28, 85, 1011
BciVI GTATCC 1 cut(s) 1273
BclI TGATCA 1 cut(s) 1441
BcoDI GTCTC 4 cut(s) 501, 659, 1082, 1247
BfaI CTAG 2 cut(s) 246, 672
BfmI CTRYAG 1 cut(s) 683
BfrI CTTAAG 1 cut(s) 1054
BfuAI ACCTGC 1 cut(s) 1545
BfuI GTATCC 1 cut(s) 1273
BisI GCNGC 4 cut(s) 698, 825, 861, 918
BlsI GCNGC 4 cut(s) 699, 826, 862, 919
BmcAI AGTACT 1 cut(s) 1142
Bme1390I CCNGG 3 cut(s) 28, 85, 1011
Bme18I GGWCC 1 cut(s) 1016
BmgBI CACGTC 1 cut(s) 1481
BmgT120I GGNCC 1 cut(s) 1016
BmiI GGNNCC 2 cut(s) 171, 1345
BmrFI CCNGG 3 cut(s) 28, 85, 1011
BmsI GCATC 4 cut(s) 315, 382, 966, 1105
BpiI GAAGAC 1 cut(s) 1012
BplI GAGNNNNNCTC 2 cut(s) 721, 753
BpmI CTGGAG 1 cut(s) 683
Bpu10I CCTNAGC 1 cut(s) 836
BpuEI CTTGAG 1 cut(s) 985
BsaJI CCNNGG 3 cut(s) 84, 214, 1274
Bsc4I CCNNNNNNNGG 6 cut(s) 84, 85, 1058, 1161, 1237, 1275
Bse1I ACTGG 3 cut(s) 73, 784, 1269
Bse3DI GCAATG 2 cut(s) 739, 991
BseBI CCWGG 3 cut(s) 28, 85, 1011
BseDI CCNNGG 3 cut(s) 84, 214, 1274
BseGI GGATG 4 cut(s) 144, 409, 573, 957
BseLI CCNNNNNNNGG 6 cut(s) 84, 85, 1058, 1161, 1237, 1275
BseMI GCAATG 2 cut(s) 739, 991
BseMII CTCAG 2 cut(s) 740, 827
BseNI ACTGG 3 cut(s) 73, 784, 1269
BseRI GAGGAG 3 cut(s) 239, 424, 461
BseSI GKGCMC 2 cut(s) 640, 1514
BseXI GCAGC 3 cut(s) 709, 811, 872
Bsh1236I CGCG 1 cut(s) 766
BshFI GGCC 2 cut(s) 979, 1531
BsiHKAI GWGCWC 2 cut(s) 35, 640
BslFI GGGAC 1 cut(s) 61
BslI CCNNNNNNNGG 6 cut(s) 84, 85, 1058, 1161, 1237, 1275
BsmAI GTCTC 4 cut(s) 501, 659, 1082, 1247
BsmFI GGGAC 1 cut(s) 61
BsnI GGCC 2 cut(s) 979, 1531
Bsp1286I GDGCHC 4 cut(s) 35, 622, 640, 1514
Bsp143I GATC 4 cut(s) 658, 1240, 1343, 1441
BspACI CCGC 3 cut(s) 266, 766, 917
BspANI GGCC 2 cut(s) 979, 1531
BspCNI CTCAG 2 cut(s) 739, 828
BspFNI CGCG 1 cut(s) 766
BspHI TCATGA 1 cut(s) 873
BspLI GGNNCC 2 cut(s) 171, 1345
BspMI ACCTGC 1 cut(s) 1545
BspPI GGATC 3 cut(s) 1248, 1338, 1351
BspQI GCTCTTC 1 cut(s) 627
BspTI CTTAAG 1 cut(s) 1054
BsrDI GCAATG 2 cut(s) 739, 991
BsrI ACTGG 3 cut(s) 73, 784, 1269
BssECI CCNNGG 3 cut(s) 84, 214, 1274
BssMI GATC 4 cut(s) 658, 1240, 1343, 1441
BssSI CACGAG 1 cut(s) 1434
BssT1I CCWWGG 1 cut(s) 1274
Bst2BI CACGAG 1 cut(s) 1434
Bst2UI CCWGG 3 cut(s) 28, 85, 1011
Bst4CI ACNGT 6 cut(s) 95, 1171, 1258, 1285, 1333, 1525
Bst6I CTCTTC 1 cut(s) 627
BstAFI CTTAAG 1 cut(s) 1054
BstC8I GCNNGC 3 cut(s) 775, 865, 981
BstDEI CTNAG 2 cut(s) 726, 836
BstEII GGTNACC 1 cut(s) 1264
BstF5I GGATG 4 cut(s) 144, 409, 573, 957
BstFNI CGCG 1 cut(s) 766
BstHHI GCGC 1 cut(s) 766
BstKTI GATC 4 cut(s) 661, 1243, 1346, 1444
BstMAI GTCTC 4 cut(s) 501, 659, 1082, 1247
BstMBI GATC 4 cut(s) 658, 1240, 1343, 1441
BstMWI GCNNNNNNNGC 6 cut(s) 490, 685, 694, 761, 770, 926
BstNI CCWGG 3 cut(s) 28, 85, 1011
BstNSI RCATGY 2 cut(s) 347, 1506
BstPI GGTNACC 1 cut(s) 1264
BstSCI CCNGG 3 cut(s) 26, 83, 1009
BstSFI CTRYAG 1 cut(s) 683
BstSLI GKGCMC 2 cut(s) 640, 1514
BstUI CGCG 1 cut(s) 766
BstV1I GCAGC 3 cut(s) 709, 811, 872
BstV2I GAAGAC 1 cut(s) 1012
BstX2I RGATCY 2 cut(s) 1240, 1343
BstYI RGATCY 2 cut(s) 1240, 1343
BsuI GTATCC 1 cut(s) 1273
BsuRI GGCC 2 cut(s) 979, 1531
BtgZI GCGATG 1 cut(s) 1082
BtrI CACGTC 1 cut(s) 1481
BtsCI GGATG 4 cut(s) 144, 409, 573, 957
BtsI GCAGTG 1 cut(s) 1555
BtsIMutI CAGTG 3 cut(s) 777, 1512, 1555
BveI ACCTGC 1 cut(s) 1545
Cac8I GCNNGC 3 cut(s) 775, 865, 981
CaiI CAGNNNCTG 1 cut(s) 827
CciI TCATGA 1 cut(s) 873
CfoI GCGC 1 cut(s) 766
Cfr13I GGNCC 1 cut(s) 1016
CsiI ACCWGGT 1 cut(s) 1009
Csp6I GTAC 2 cut(s) 1141, 1558
CviAII CATG 5 cut(s) 344, 874, 1352, 1503, 1566
CviQI GTAC 2 cut(s) 1141, 1558
DdeI CTNAG 2 cut(s) 726, 836
DpnI GATC 4 cut(s) 660, 1242, 1345, 1443
DpnII GATC 4 cut(s) 658, 1240, 1343, 1441
DraI TTTAAA 1 cut(s) 912
DrdI GACNNNNNNGTC 1 cut(s) 515
DseDI GACNNNNNNGTC 1 cut(s) 515
Eam1104I CTCTTC 1 cut(s) 627
EarI CTCTTC 1 cut(s) 627
Ecl136II GAGCTC 1 cut(s) 33
Eco130I CCWWGG 1 cut(s) 1274
Eco24I GRGCYC 2 cut(s) 35, 622
Eco47I GGWCC 1 cut(s) 1016
Eco53kI GAGCTC 1 cut(s) 33
Eco91I GGTNACC 1 cut(s) 1264
EcoICRI GAGCTC 1 cut(s) 33
EcoO65I GGTNACC 1 cut(s) 1264
EcoRII CCWGG 3 cut(s) 26, 83, 1009
EcoT14I CCWWGG 1 cut(s) 1274
EcoT38I GRGCYC 2 cut(s) 35, 622
ErhI CCWWGG 1 cut(s) 1274
FaeI CATG 5 cut(s) 347, 877, 1355, 1506, 1569
FaqI GGGAC 1 cut(s) 61
FatI CATG 5 cut(s) 343, 873, 1351, 1502, 1565
FauNDI CATATG 2 cut(s) 283, 903
FbaI TGATCA 1 cut(s) 1441
Fnu4HI GCNGC 4 cut(s) 698, 825, 861, 918
FokI GGATG 4 cut(s) 151, 416, 580, 944
FriOI GRGCYC 2 cut(s) 35, 622
Fsp4HI GCNGC 4 cut(s) 698, 825, 861, 918
FspBI CTAG 2 cut(s) 246, 672
GlaI GCGC 1 cut(s) 765
GluI GCNGC 4 cut(s) 698, 825, 861, 918
GsuI CTGGAG 1 cut(s) 683
HaeIII GGCC 2 cut(s) 979, 1531
HhaI GCGC 1 cut(s) 766
Hin1II CATG 5 cut(s) 347, 877, 1355, 1506, 1569
Hin6I GCGC 1 cut(s) 764
HinP1I GCGC 1 cut(s) 764
HinfI GANTC 4 cut(s) 583, 1080, 1309, 1421
HphI GGTGA 2 cut(s) 464, 728
Hpy166II GTNNAC 2 cut(s) 638, 1016
Hpy188I TCNGA 5 cut(s) 463, 525, 729, 1379, 1446
Hpy8I GTNNAC 2 cut(s) 638, 1016
HpyAV CCTTC 5 cut(s) 90, 116, 150, 810, 1369
HpyCH4III ACNGT 6 cut(s) 95, 1171, 1258, 1285, 1333, 1525
HpyCH4IV ACGT 1 cut(s) 1480
HpyCH4V TGCA 8 cut(s) 43, 205, 328, 638, 723, 749, 860, 1355
HpyF10VI GCNNNNNNNGC 6 cut(s) 490, 685, 694, 761, 770, 926
HpyF3I CTNAG 2 cut(s) 726, 836
HpySE526I ACGT 1 cut(s) 1480
Hsp92II CATG 5 cut(s) 347, 877, 1355, 1506, 1569
HspAI GCGC 1 cut(s) 764
Ksp22I TGATCA 1 cut(s) 1441
Kzo9I GATC 4 cut(s) 658, 1240, 1343, 1441
LguI GCTCTTC 1 cut(s) 627
LmnI GCTCC 3 cut(s) 16, 30, 530
Lsp1109I GCAGC 3 cut(s) 709, 811, 872
LweI GCATC 4 cut(s) 315, 382, 966, 1105
MabI ACCWGGT 1 cut(s) 1009
MaeI CTAG 2 cut(s) 246, 672
MaeII ACGT 1 cut(s) 1480
MaeIII GTNAC 3 cut(s) 319, 413, 1264
MalI GATC 4 cut(s) 660, 1242, 1345, 1443
MboI GATC 4 cut(s) 658, 1240, 1343, 1441
MboII GAAGA 9 cut(s) 233, 398, 610, 614, 798, 883, 889, 1017, 1085
MflI RGATCY 2 cut(s) 1240, 1343
MhlI GDGCHC 4 cut(s) 35, 622, 640, 1514
MlyI GAGTC 2 cut(s) 592, 1089
MmeI TCCRAC 1 cut(s) 532
MseI TTAA 8 cut(s) 210, 887, 911, 1055, 1125, 1218, 1236, 1295
MslI CAYNNNNRTG 2 cut(s) 348, 878
MspA1I CMGCKG 1 cut(s) 824
MspCI CTTAAG 1 cut(s) 1054
MspR9I CCNGG 3 cut(s) 28, 85, 1011
MvaI CCWGG 3 cut(s) 28, 85, 1011
MvnI CGCG 1 cut(s) 766
MwoI GCNNNNNNNGC 6 cut(s) 490, 685, 694, 761, 770, 926
NdeI CATATG 2 cut(s) 283, 903
NdeII GATC 4 cut(s) 658, 1240, 1343, 1441
NlaIII CATG 5 cut(s) 347, 877, 1355, 1506, 1569
NlaIV GGNNCC 2 cut(s) 171, 1345
NspI RCATGY 2 cut(s) 347, 1506
PagI TCATGA 1 cut(s) 873
PciSI GCTCTTC 1 cut(s) 627
PfeI GAWTC 2 cut(s) 1309, 1421
PflMI CCANNNNNTGG 1 cut(s) 1161
PkrI GCNGC 4 cut(s) 699, 826, 862, 919
PleI GAGTC 2 cut(s) 591, 1088
PpsI GAGTC 2 cut(s) 591, 1088
Psp124BI GAGCTC 1 cut(s) 35
Psp6I CCWGG 3 cut(s) 26, 83, 1009
PspEI GGTNACC 1 cut(s) 1264
PspGI CCWGG 3 cut(s) 26, 83, 1009
PspN4I GGNNCC 2 cut(s) 171, 1345
PspPI GGNCC 1 cut(s) 1016
PstNI CAGNNNCTG 1 cut(s) 827
PsuI RGATCY 2 cut(s) 1240, 1343
PvuII CAGCTG 1 cut(s) 824
RsaI GTAC 2 cut(s) 1142, 1559
RsaNI GTAC 2 cut(s) 1141, 1558
RseI CAYNNNNRTG 2 cut(s) 348, 878
SacI GAGCTC 1 cut(s) 35
SapI GCTCTTC 1 cut(s) 627
SaqAI TTAA 8 cut(s) 210, 887, 911, 1055, 1125, 1218, 1236, 1295
SatI GCNGC 4 cut(s) 698, 825, 861, 918
Sau3AI GATC 4 cut(s) 658, 1240, 1343, 1441
Sau96I GGNCC 1 cut(s) 1016
ScaI AGTACT 1 cut(s) 1142
SchI GAGTC 2 cut(s) 592, 1089
ScrFI CCNGG 3 cut(s) 28, 85, 1011
SduI GDGCHC 4 cut(s) 35, 622, 640, 1514
SexAI ACCWGGT 1 cut(s) 1009
SfaNI GCATC 4 cut(s) 315, 382, 966, 1105
SfcI CTRYAG 1 cut(s) 683
SinI GGWCC 1 cut(s) 1016
SmiMI CAYNNNNRTG 2 cut(s) 348, 878
SmlI CTYRAG 2 cut(s) 1000, 1054
SmoI CTYRAG 2 cut(s) 1000, 1054
SsiI CCGC 3 cut(s) 266, 766, 917
SspI AATATT 1 cut(s) 1389
SspMI CTAG 2 cut(s) 246, 672
SstI GAGCTC 1 cut(s) 35
StyD4I CCNGG 3 cut(s) 26, 83, 1009
StyI CCWWGG 1 cut(s) 1274
TaaI ACNGT 6 cut(s) 95, 1171, 1258, 1285, 1333, 1525
TaiI ACGT 1 cut(s) 1483
TaqI TCGA 2 cut(s) 1066, 1137
TatI WGTACW 1 cut(s) 1140
TauI GCSGC 1 cut(s) 920
TfiI GAWTC 2 cut(s) 1309, 1421
Tru1I TTAA 8 cut(s) 210, 887, 911, 1055, 1125, 1218, 1236, 1295
Tru9I TTAA 8 cut(s) 210, 887, 911, 1055, 1125, 1218, 1236, 1295
TscAI CASTG 3 cut(s) 784, 1519, 1555
TseI GCWGC 3 cut(s) 697, 824, 860
TspDTI ATGAA 5 cut(s) 207, 862, 890, 929, 980
TspRI CASTG 3 cut(s) 784, 1519, 1555
Van91I CCANNNNNTGG 1 cut(s) 1161
Vha464I CTTAAG 1 cut(s) 1054
VneI GTGCAC 1 cut(s) 636
VpaK11BI GGWCC 1 cut(s) 1016
XapI RAATTY 1 cut(s) 1291
XceI RCATGY 2 cut(s) 347, 1506
XspI CTAG 2 cut(s) 246, 672
ZrmI AGTACT 1 cut(s) 1142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.