Rorug05G0372500

oxidosqualene cyclase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000624
Physical Location & Seq
Forward (+)
50218448 .. 50221034
2587 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0372500.1

Sequence Viewer

Length: 1623 bp
ATGGAGCTTGTTCAGCAAAGTCATCCGCATCCCAAGGCTTCTGTTACCCAAGTTACTGCTTACGGCGGTAGCCCTAGTCCTATAAAGCAACTGCAACCAATTCCAAATGAAGCTGATCGCAAGGCTTCTTTGACCGGGACGCATGATGCCATTGTTGTGTCCTCTGTTGCAGCAGAGGTCGATCAGTTTTCGGACGTGGAAGAGGAGCTGAACAATTTCAATAACAATCAGAAAAGGCAGCAGGATGCTTTTCTGAACAGTTTTCCACCTGGGTATAGGTTCAATCCTCGTGATGACGAACTTGTTCGGTATTACCTGCAGAAGAAGATCATGGACGAGCCATTGCCCCCCAACAAGATCATGGAAGTTAACCTCTACCGCCACAATCCCGAGACGCTTGCTGAAAGATATGAAACTTATGGAGAAAAAGAATGGTACTTCTTTACTCCAAGGGACCGGAAGTATCGCAATGGATTTCGACCAAATAGGGCAGCTGGTGATGGATATTGGAAGGCCACAGGAGCTGACAAAACAATAAGGTCTGGCAATGTAGAGGTTGGGTGTAGAAAGGCTCTCGTTTTTTACAGAGGTAAACCTCCAAAGGGTAAAAAGACGAATTGGATCATGCATGAGTATCGAGTCCCTGATTCCCCTGATTCTCGTCCTCCCAAAAGGACTGAAAATGACATGAGGTTGGATAACTGGGTGCTGTGTAGAATTTATAAGAAAGCTGATAACAAGAACAACAAAAGCCAGAGCCGAAATCATGTAGAGGATCCTGTTGAAGATACATCACTACCAGATAACATGGACATGGAGATGGAGACGAACGGTCAAGTTGATAACCCTCAAGCATTGGAGTCTGAAAAGGCATACCCTTACCCTATTGTTCATCCACCATTGGAGTTTGACAGGGCGTATCCTAATTATGCATTTCCTTACCCGGGTGGGCGGCCTCTATGTGCTCTAGTTAACAACAATATGAACAACATGCTTCATCACCACCGATTCCAACCAGTCTCTTTGAATTCTGGGGATAGTTATGCAAATATGGGGCAAATGCAACAGGCTTGTGGCACTTATTCACCAATGCAATTTCCTGATCAAAACTGTGGTGTGCAATTTTCTGATCAAAAGCGTGGTGTGCAATTTTCGGACCAAAGGTTTATTATGCAACCAGTTGATGTCACATCAGGATCAATGGTATGTACAGGTGATCAAAAGTGTGCTGTTCAATTTCCAGACGAAAAGTTTATTATGCAATCACTTGATGTCACATCAGAATCAATAGTATGTACAGATGAGGCATGTTCTGATCAGAAATGTGGTAGGCAATTTCCGGATCAAAAATTTATTGTGCAATCACCTGATTGTAGCCCACAACCAATGACATTTTCTCATGAAAAAAGTGAAAAGAGTGTCCAACCAGATGATGCAGTTGATAGTAGACCTTCATCAGTGCCTTCTTTACCTGATCAAAAGAGTACTCAACAAGGAATTGGTAAGCCTCTATTACCACCATCTGATCAAGGTCTACGATACGTAGAAGAATCGAATGAAGATCATAATCTCATGTTTGGTTATCCACCATTTGCGTCTTTGGAAACTTTTTCATGGCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 map00100 map00909 map01100 map01110
Pfam Domains
Protein Families
Prenyltrans SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

540

Amino Acids

61.5

Weight (kDa)

5.5

Isoelectric Point (pI)

42.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 89 - 214 1.8e-44 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 723
Acc36I ACCTGC 1 cut(s) 324
AccI GTMKAC 2 cut(s) 1447, 1534
AccIII TCCGGA 1 cut(s) 1339
AciI CCGC 4 cut(s) 26, 66, 379, 952
AclWI GGATC 5 cut(s) 629, 770, 783, 1204, 1350
AcsI RAATTY 3 cut(s) 717, 1027, 1349
AfaI GTAC 4 cut(s) 437, 1210, 1297, 1486
AfiI CCNNNNNNNGG 2 cut(s) 602, 944
AgsI TTSAA 5 cut(s) 220, 283, 785, 1027, 1235
AhdI GACNNNNNGTC 1 cut(s) 831
AjiI CACGTC 1 cut(s) 196
AjnI CCWGG 1 cut(s) 268
AjuI GAANNNNNNNTTGG 2 cut(s) 1378, 1410
AluBI AGCT 6 cut(s) 7, 113, 208, 494, 524, 731
AluI AGCT 6 cut(s) 7, 113, 208, 494, 524, 731
Alw21I GWGCWC 1 cut(s) 967
Alw26I GTCTC 3 cut(s) 386, 818, 1024
AlwI GGATC 5 cut(s) 629, 770, 783, 1204, 1350
AlwNI CAGNNNCTG 1 cut(s) 524
Ama87I CYCGRG 2 cut(s) 389, 943
Aor13HI TCCGGA 1 cut(s) 1339
AoxI GGCC 3 cut(s) 513, 953, 1616
ApeKI GCWGC 3 cut(s) 170, 238, 491
ApoI RAATTY 3 cut(s) 717, 1027, 1349
Asp700I GAANNNNTTC 2 cut(s) 215, 303
AspS9I GGNCC 3 cut(s) 454, 1156, 1617
AsuC2I CCSGG 3 cut(s) 136, 944, 945
AsuHPI GGTGA 5 cut(s) 509, 992, 1077, 1226, 1356
AvaI CYCGRG 2 cut(s) 389, 943
AvaII GGWCC 2 cut(s) 454, 1156
BamHI GGATCC 1 cut(s) 775
BauI CACGAG 1 cut(s) 288
Bbv12I GWGCWC 1 cut(s) 967
BbvI GCAGC 3 cut(s) 182, 250, 503
BccI CCATC 3 cut(s) 494, 814, 1528
BceAI ACGGC 1 cut(s) 79
BcgI CGANNNNNNTGC 4 cut(s) 380, 414, 617, 651
BciT130I CCWGG 1 cut(s) 270
BciVI GTATCC 1 cut(s) 930
BclI TGATCA 6 cut(s) 1102, 1129, 1216, 1315, 1474, 1525
BcnI CCSGG 3 cut(s) 136, 944, 945
BcoDI GTCTC 3 cut(s) 386, 818, 1024
BfaI CTAG 2 cut(s) 75, 968
BfmI CTRYAG 1 cut(s) 317
BfuAI ACCTGC 1 cut(s) 324
BfuI GTATCC 1 cut(s) 930
BisI GCNGC 4 cut(s) 171, 239, 492, 953
BlsI GCNGC 4 cut(s) 172, 240, 493, 954
BmcAI AGTACT 1 cut(s) 1486
Bme1390I CCNGG 4 cut(s) 136, 270, 944, 945
Bme18I GGWCC 2 cut(s) 454, 1156
BmeRI GACNNNNNGTC 1 cut(s) 831
BmeT110I CYCGRG 2 cut(s) 389, 943
BmgBI CACGTC 1 cut(s) 196
BmgT120I GGNCC 3 cut(s) 454, 1156, 1617
BmiI GGNNCC 2 cut(s) 455, 777
BmrFI CCNGG 4 cut(s) 136, 270, 944, 945
BmrI ACTGGG 1 cut(s) 712
BmsI GCATC 4 cut(s) 37, 136, 235, 1423
BmuI ACTGGG 1 cut(s) 712
BpuEI CTTGAG 1 cut(s) 834
BpuMI CCSGG 3 cut(s) 136, 944, 945
BsaAI YACGTR 1 cut(s) 1543
BsaBI GATNNNNATC 1 cut(s) 1566
BsaJI CCNNGG 4 cut(s) 33, 269, 449, 943
BsaWI WCCGGW 2 cut(s) 456, 1339
Bsc4I CCNNNNNNNGG 2 cut(s) 602, 944
Bse1I ACTGG 3 cut(s) 707, 1016, 1178
Bse3DI GCAATG 3 cut(s) 341, 475, 553
Bse8I GATNNNNATC 1 cut(s) 1566
BseAI TCCGGA 1 cut(s) 1339
BseBI CCWGG 1 cut(s) 270
BseDI CCNNGG 4 cut(s) 33, 269, 449, 943
BseGI GGATG 4 cut(s) 22, 28, 250, 892
BseJI GATNNNNATC 1 cut(s) 1566
BseLI CCNNNNNNNGG 2 cut(s) 602, 944
BseMI GCAATG 3 cut(s) 341, 475, 553
BseNI ACTGG 3 cut(s) 707, 1016, 1178
BseRI GAGGAG 1 cut(s) 218
BseXI GCAGC 3 cut(s) 182, 250, 503
BshFI GGCC 3 cut(s) 515, 955, 1618
BsiHKAI GWGCWC 1 cut(s) 967
BsiHKCI CYCGRG 2 cut(s) 389, 943
BsiSI CCGG 4 cut(s) 135, 457, 944, 1340
BslFI GGGAC 3 cut(s) 151, 467, 626
BslI CCNNNNNNNGG 2 cut(s) 602, 944
BsmAI GTCTC 3 cut(s) 386, 818, 1024
BsmBI CGTCTC 2 cut(s) 386, 818
BsmFI GGGAC 3 cut(s) 151, 467, 626
BsnI GGCC 3 cut(s) 515, 955, 1618
BsoBI CYCGRG 2 cut(s) 389, 943
Bsp1286I GDGCHC 1 cut(s) 967
Bsp13I TCCGGA 1 cut(s) 1339
Bsp1407I TGTACA 2 cut(s) 1208, 1295
BspACI CCGC 4 cut(s) 26, 66, 379, 952
BspANI GGCC 3 cut(s) 515, 955, 1618
BspEI TCCGGA 1 cut(s) 1339
BspHI TCATGA 1 cut(s) 1399
BspLI GGNNCC 2 cut(s) 455, 777
BspMAI CTGCAG 1 cut(s) 321
BspMI ACCTGC 1 cut(s) 324
BspPI GGATC 5 cut(s) 629, 770, 783, 1204, 1350
BsrDI GCAATG 3 cut(s) 341, 475, 553
BsrGI TGTACA 2 cut(s) 1208, 1295
BsrI ACTGG 3 cut(s) 707, 1016, 1178
BssECI CCNNGG 4 cut(s) 33, 269, 449, 943
BssSI CACGAG 1 cut(s) 288
BssT1I CCWWGG 2 cut(s) 33, 449
Bst2BI CACGAG 1 cut(s) 288
Bst2UI CCWGG 1 cut(s) 270
Bst4CI ACNGT 3 cut(s) 260, 833, 1112
Bst6I CTCTTC 1 cut(s) 195
BstAUI TGTACA 2 cut(s) 1208, 1295
BstBAI YACGTR 1 cut(s) 1543
BstC8I GCNNGC 1 cut(s) 399
BstF5I GGATG 4 cut(s) 22, 28, 250, 892
BstMAI GTCTC 3 cut(s) 386, 818, 1024
BstMWI GCNNNNNNNGC 3 cut(s) 13, 521, 1144
BstNI CCWGG 1 cut(s) 270
BstNSI RCATGY 2 cut(s) 994, 1311
BstSCI CCNGG 4 cut(s) 134, 268, 942, 943
BstSFI CTRYAG 1 cut(s) 317
BstSNI TACGTA 1 cut(s) 1543
BstV1I GCAGC 3 cut(s) 182, 250, 503
BstX2I RGATCY 1 cut(s) 775
BstYI RGATCY 1 cut(s) 775
BsuI GTATCC 1 cut(s) 930
BsuRI GGCC 3 cut(s) 515, 955, 1618
BtrI CACGTC 1 cut(s) 196
BtsCI GGATG 4 cut(s) 22, 28, 250, 892
BtsIMutI CAGTG 1 cut(s) 1464
BveI ACCTGC 1 cut(s) 324
Cac8I GCNNGC 1 cut(s) 399
CaiI CAGNNNCTG 1 cut(s) 524
CciI TCATGA 1 cut(s) 1399
Cfr13I GGNCC 3 cut(s) 454, 1156, 1617
Cfr9I CCCGGG 1 cut(s) 943
CseI GACGC 3 cut(s) 148, 403, 1584
Csp6I GTAC 4 cut(s) 436, 1209, 1296, 1485
CviQI GTAC 4 cut(s) 436, 1209, 1296, 1485
DriI GACNNNNNGTC 1 cut(s) 831
Eam1104I CTCTTC 1 cut(s) 195
Eam1105I GACNNNNNGTC 1 cut(s) 831
EarI CTCTTC 1 cut(s) 195
Eco105I TACGTA 1 cut(s) 1543
Eco130I CCWWGG 2 cut(s) 33, 449
Eco47I GGWCC 2 cut(s) 454, 1156
Eco88I CYCGRG 2 cut(s) 389, 943
EcoRI GAATTC 1 cut(s) 1027
EcoRII CCWGG 1 cut(s) 268
EcoT14I CCWWGG 2 cut(s) 33, 449
EcoT22I ATGCAT 2 cut(s) 630, 934
ErhI CCWWGG 2 cut(s) 33, 449
Esp3I CGTCTC 2 cut(s) 386, 818
FaqI GGGAC 3 cut(s) 151, 467, 626
FbaI TGATCA 6 cut(s) 1102, 1129, 1216, 1315, 1474, 1525
FblI GTMKAC 2 cut(s) 1447, 1534
Fnu4HI GCNGC 4 cut(s) 171, 239, 492, 953
FokI GGATG 4 cut(s) 9, 15, 257, 879
Fsp4HI GCNGC 4 cut(s) 171, 239, 492, 953
FspBI CTAG 2 cut(s) 75, 968
GluI GCNGC 4 cut(s) 171, 239, 492, 953
HaeIII GGCC 3 cut(s) 515, 955, 1618
HapII CCGG 4 cut(s) 135, 457, 944, 1340
HgaI GACGC 3 cut(s) 148, 403, 1584
HincII GTYRAC 2 cut(s) 370, 973
HindII GTYRAC 2 cut(s) 370, 973
HinfI GANTC 7 cut(s) 639, 647, 656, 860, 1008, 1283, 1550
HpaI GTTAAC 2 cut(s) 370, 973
HpaII CCGG 4 cut(s) 135, 457, 944, 1340
HphI GGTGA 5 cut(s) 509, 992, 1077, 1226, 1356
Hpy166II GTNNAC 5 cut(s) 370, 593, 973, 1448, 1535
Hpy188III TCNNGA 7 cut(s) 290, 389, 1100, 1194, 1241, 1340, 1400
Hpy8I GTNNAC 5 cut(s) 370, 593, 973, 1448, 1535
HpyAV CCTTC 3 cut(s) 505, 1461, 1473
HpyCH4III ACNGT 3 cut(s) 260, 833, 1112
HpyCH4IV ACGT 2 cut(s) 195, 1542
HpyF10VI GCNNNNNNNGC 3 cut(s) 13, 521, 1144
HpySE526I ACGT 2 cut(s) 195, 1542
Kpn2I TCCGGA 1 cut(s) 1339
Ksp22I TGATCA 6 cut(s) 1102, 1129, 1216, 1315, 1474, 1525
KspAI GTTAAC 2 cut(s) 370, 973
LmnI GCTCC 3 cut(s) 4, 205, 521
Lsp1109I GCAGC 3 cut(s) 182, 250, 503
LweI GCATC 4 cut(s) 37, 136, 235, 1423
MaeI CTAG 2 cut(s) 75, 968
MaeII ACGT 2 cut(s) 195, 1542
MaeIII GTNAC 4 cut(s) 43, 52, 1186, 1273
MboII GAAGA 6 cut(s) 212, 334, 337, 797, 1559, 1571
MflI RGATCY 1 cut(s) 775
MhlI GDGCHC 1 cut(s) 967
MlyI GAGTC 2 cut(s) 648, 869
MmeI TCCRAC 3 cut(s) 675, 1036, 1447
Mph1103I ATGCAT 2 cut(s) 630, 934
MroI TCCGGA 1 cut(s) 1339
MroXI GAANNNNTTC 2 cut(s) 215, 303
MseI TTAA 2 cut(s) 369, 972
MslI CAYNNNNRTG 3 cut(s) 155, 812, 818
MspA1I CMGCKG 1 cut(s) 494
MspI CCGG 4 cut(s) 135, 457, 944, 1340
MspR9I CCNGG 4 cut(s) 136, 270, 944, 945
MvaI CCWGG 1 cut(s) 270
MwoI GCNNNNNNNGC 3 cut(s) 13, 521, 1144
NciI CCSGG 3 cut(s) 136, 944, 945
NlaIV GGNNCC 2 cut(s) 455, 777
NmuCI GTSAC 2 cut(s) 1186, 1273
NsiI ATGCAT 2 cut(s) 630, 934
NspI RCATGY 2 cut(s) 994, 1311
PagI TCATGA 1 cut(s) 1399
PdmI GAANNNNTTC 2 cut(s) 215, 303
PfeI GAWTC 5 cut(s) 647, 656, 1008, 1283, 1550
PkrI GCNGC 4 cut(s) 172, 240, 493, 954
PleI GAGTC 2 cut(s) 647, 868
PpsI GAGTC 2 cut(s) 647, 868
Ppu21I YACGTR 1 cut(s) 1543
PsiI TTATAA 1 cut(s) 723
Psp6I CCWGG 1 cut(s) 268
PspGI CCWGG 1 cut(s) 268
PspN4I GGNNCC 2 cut(s) 455, 777
PspPI GGNCC 3 cut(s) 454, 1156, 1617
PstI CTGCAG 1 cut(s) 321
PstNI CAGNNNCTG 1 cut(s) 524
PsuI RGATCY 1 cut(s) 775
PvuII CAGCTG 1 cut(s) 494
RsaI GTAC 4 cut(s) 437, 1210, 1297, 1486
RsaNI GTAC 4 cut(s) 436, 1209, 1296, 1485
RseI CAYNNNNRTG 3 cut(s) 155, 812, 818
SaqAI TTAA 2 cut(s) 369, 972
SatI GCNGC 4 cut(s) 171, 239, 492, 953
Sau96I GGNCC 3 cut(s) 454, 1156, 1617
ScaI AGTACT 1 cut(s) 1486
SchI GAGTC 2 cut(s) 648, 869
ScrFI CCNGG 4 cut(s) 136, 270, 944, 945
SduI GDGCHC 1 cut(s) 967
SfaNI GCATC 4 cut(s) 37, 136, 235, 1423
SfcI CTRYAG 1 cut(s) 317
SinI GGWCC 2 cut(s) 454, 1156
SmaI CCCGGG 1 cut(s) 945
SmiMI CAYNNNNRTG 3 cut(s) 155, 812, 818
SmlI CTYRAG 1 cut(s) 849
SmoI CTYRAG 1 cut(s) 849
SnaBI TACGTA 1 cut(s) 1543
SsiI CCGC 4 cut(s) 26, 66, 379, 952
SspMI CTAG 2 cut(s) 75, 968
StyD4I CCNGG 4 cut(s) 134, 268, 942, 943
StyI CCWWGG 2 cut(s) 33, 449
TaaI ACNGT 3 cut(s) 260, 833, 1112
TaiI ACGT 2 cut(s) 198, 1545
TaqI TCGA 4 cut(s) 180, 478, 637, 1553
TatI WGTACW 3 cut(s) 1208, 1295, 1484
TauI GCSGC 1 cut(s) 955
TfiI GAWTC 5 cut(s) 647, 656, 1008, 1283, 1550
Tru1I TTAA 2 cut(s) 369, 972
Tru9I TTAA 2 cut(s) 369, 972
TscAI CASTG 1 cut(s) 1464
TseFI GTSAC 2 cut(s) 1186, 1273
TseI GCWGC 3 cut(s) 170, 238, 491
Tsp45I GTSAC 2 cut(s) 1186, 1273
TspDTI ATGAA 9 cut(s) 123, 426, 881, 986, 998, 1416, 1443, 1572, 1602
TspMI CCCGGG 1 cut(s) 943
TspRI CASTG 1 cut(s) 1464
VpaK11BI GGWCC 2 cut(s) 454, 1156
XapI RAATTY 3 cut(s) 717, 1027, 1349
XceI RCATGY 2 cut(s) 994, 1311
XcmI CCANNNNNNNNNTGG 1 cut(s) 358
XmaI CCCGGG 1 cut(s) 943
XmiI GTMKAC 2 cut(s) 1447, 1534
XmnI GAANNNNTTC 2 cut(s) 215, 303
XspI CTAG 2 cut(s) 75, 968
ZrmI AGTACT 1 cut(s) 1486
Zsp2I ATGCAT 2 cut(s) 630, 934
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.