RchiOBHm_Chr5g0065531

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000624
Physical Location & Seq
Forward (+)
71487409 .. 71490520
3112 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34139

Sequence Viewer

Length: 1809 bp
ATGGATCCACGTTTCTCTGAATTTCCCAGTTTCAATGATCAAACCCTTTTGCCTGATATGTTGAGTGAGTACCCAAGTAGTGATCCTTCTCAAGATCTTAGCTTTTTGGACCCAAATCCCAGTAACTCTTCATCAAGCTTTAGCCCTGAGGGAGATGATAGTGAGTTTTCTGACAGTGTCCTCAAGTACATAAACCAGGTGCTTATTAAGGAGGAAATGCAATCAAAACCCTGTATGTTCCATGACCCTTTGGCTCTTCAAGCCACTGAGGAGTCTCTTCATGAGATTATTGGAGGAGGGAAAAACCCAACTTCCCAACACCTAAACCCTCTTTGTGTTGATCAAAATGTGTATGGAAATGGGAATGGGAATGGGATGCTGGGTTCTTATAGGAGTGAGCTAATTGTTCCTGATATGCTTAGTAAGAGTGAATTGTTATTGCAATTTAACCTAGGGGCAGAGGAGGGTAGTAAGTTTCTTCCTAGAGGTCAATTGATTAGTGCAGAGGGCAACAAGCCGTATGCTGTGGCAGATAGAAAGGCTGGAGATGTGGTAGTCCCGACCCAGAAGAATGGGAGTGAGCATTTTCGTGTTGGTTCTAGAGGAAAGAAAAATCATCAAAGGGAAGATATAGATTTGGAGGATGGGAGGAGTAACAAGCAATCGGCGGTTTATATGGATGGTGAGGAGGGTGAGCTGTCTCATATTTTTGACAAGGTGTTGCTCTCCAAGCCTATCAAGAGTAAAGACGATCAAGTCAGTCAGAATGGAGCAAACAATAGCCCGCAGCAGGATGGGCTATCTGTTGGAATTGGTAATGGGAAGGTTTGTGGCAAGAATCAAGGCAACAAGAAGGAAGTGATAGATTTGAGGGCTCTTCTGATTTCATCTGCTGATTCGCTGAAGTTTTACCAAGCTTATATTGCAGTCTGCCCGTTCATGAAGTTGTTGATGATCTTTGCAAACGATATGATTTTAAAAGCTGCTGAGAAAGCAGAAACACTACATATAATAGATTTTGGTATCCGTTATGGTTTTCAATGGCCAGCTCTCATTCATTGCCTCTCAAGAAGACCTGGTGGACCTCCGAAATTACGCATTACAGGGTTAGAGTTTCCCTCAAGTGGTTTTCGACCAGAAGAAGGAGTCCAAGAGACAGGGCATCGCTTGGCAAAGTATTGTAGGCGATTTAATGTTCCTTTTGAGTACTATGGCATAGCCAAAAAATGGGAAACTCTCCAATTTGATGACCTTAGAGTTCAAAGAAATGAGGTGCTTGCTGTTAATTGTCTCAACCGATTCAAGTACCTCCTTGATGAGACTGTTGTGGTAAACAGTCCACGGGATACTGTTCTAAATTTAATTAGGAATTTGAATCCTGATGTCTTTGTCCAGAGTGTTGTTAATGGATCCCATCATGCACCCTTCTTTGTTCCACGCTTCCGAGAGGCACTATTCCACTTCTCTGCATTGTTTGATATGTTGGATACCAATTTGCCTCGTGAAGATCAGATGAGATTGGGGTTTGAGAAAGAGTTTATTGGACGTGAAGTTGTGAATATAGTAGCTTGTGAGGGCTTTCAAAGAATTGTTAGGCCAGAAACATACAAGCAGTGGCAGATTCGAAACATGAGGGCTGGGTTCAGACAGCTGCCATTGGACCGTGAAGTCATGAACAAAATAAAGGATAAGGTAAAAATGGGGTACCACCCTTCTTTTGTGGTTGATGAAGATGGCCACTGGATGCTGCATGAATGGAAGGGCAGAATTCTGTATGCTTCCGCCTGTTGGGCACATTCTAGGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

602

Amino Acids

68.15

Weight (kDa)

6.1

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 297 - 597 4.4e-96 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 755, 1667
Acc65I GGTACC 1 cut(s) 1704
AccB1I GGYRCC 1 cut(s) 1704
AccB7I CCANNNNNTGG 1 cut(s) 1227
AciI CCGC 3 cut(s) 668, 785, 1782
AclWI GGATC 4 cut(s) 12, 77, 1404, 1417
AcoI YGGCCR 2 cut(s) 1043, 1735
AcsI RAATTY 4 cut(s) 20, 1357, 1369, 1767
AcuI CTGAAG 1 cut(s) 923
AfaI GTAC 5 cut(s) 71, 188, 1208, 1307, 1706
AfiI CCNNNNNNNGG 5 cut(s) 790, 1124, 1142, 1227, 1788
AgsI TTSAA 7 cut(s) 34, 260, 1040, 1262, 1303, 1375, 1583
AjiI CACGTC 1 cut(s) 1547
AjnI CCWGG 2 cut(s) 195, 1075
AloI GAACNNNNNNTCC 2 cut(s) 367, 399
AluBI AGCT 9 cut(s) 102, 138, 400, 697, 917, 983, 1049, 1568, 1651
AluI AGCT 9 cut(s) 102, 138, 400, 697, 917, 983, 1049, 1568, 1651
Alw26I GTCTC 5 cut(s) 279, 705, 1148, 1295, 1313
AlwI GGATC 4 cut(s) 12, 77, 1404, 1417
AoxI GGCC 3 cut(s) 1043, 1595, 1735
ApeKI GCWGC 4 cut(s) 787, 983, 1651, 1747
ApoI RAATTY 4 cut(s) 20, 1357, 1369, 1767
Asp718I GGTACC 1 cut(s) 1704
AspA2I CCTAGG 1 cut(s) 451
AspS9I GGNCC 3 cut(s) 109, 1082, 1660
AsuHPI GGTGA 2 cut(s) 695, 704
AsuII TTCGAA 1 cut(s) 1624
AvaII GGWCC 3 cut(s) 109, 1082, 1660
AvrII CCTAGG 1 cut(s) 451
AxyI CCTNAGG 1 cut(s) 147
BaeGI GKGCMC 1 cut(s) 1795
BalI TGGCCA 2 cut(s) 1045, 1737
BamHI GGATCC 2 cut(s) 4, 1409
BanI GGYRCC 1 cut(s) 1704
BanII GRGCYC 1 cut(s) 877
BarI GAAGNNNNNNTAC 2 cut(s) 70, 102
BauI CACGAG 1 cut(s) 1500
BbsI GAAGAC 1 cut(s) 1078
BbvI GCAGC 4 cut(s) 799, 970, 1638, 1734
BccI CCATC 5 cut(s) 638, 674, 788, 1422, 1727
BceAI ACGGC 1 cut(s) 502
BciT130I CCWGG 2 cut(s) 197, 1077
BciVI GTATCC 3 cut(s) 1034, 1339, 1480
BclI TGATCA 2 cut(s) 37, 340
BcoDI GTCTC 5 cut(s) 279, 705, 1148, 1295, 1313
BfaI CTAG 4 cut(s) 452, 483, 600, 1800
BfuI GTATCC 3 cut(s) 1034, 1339, 1480
BglI GCCNNNNNGGC 1 cut(s) 1790
BglII AGATCT 1 cut(s) 94
BisI GCNGC 4 cut(s) 788, 984, 1652, 1748
BlnI CCTAGG 1 cut(s) 451
BlsI GCNGC 4 cut(s) 789, 985, 1653, 1749
BmcAI AGTACT 1 cut(s) 1208
Bme1390I CCNGG 2 cut(s) 197, 1077
Bme18I GGWCC 3 cut(s) 109, 1082, 1660
BmgBI CACGTC 1 cut(s) 1547
BmgT120I GGNCC 3 cut(s) 109, 1082, 1660
BmiI GGNNCC 4 cut(s) 6, 111, 1411, 1706
BmrFI CCNGG 2 cut(s) 197, 1077
BmrI ACTGGG 2 cut(s) 21, 114
BmsI GCATC 3 cut(s) 366, 1171, 1734
BmuI ACTGGG 2 cut(s) 21, 114
BpiI GAAGAC 1 cut(s) 1078
BplI GAGNNNNNCTC 2 cut(s) 1103, 1135
BpmI CTGGAG 1 cut(s) 564
Bpu14I TTCGAA 1 cut(s) 1624
BpuEI CTTGAG 4 cut(s) 75, 167, 1051, 1105
BsaJI CCNNGG 2 cut(s) 451, 1340
BsaXI ACNNNNNCTCC 2 cut(s) 537, 567
Bsc4I CCNNNNNNNGG 5 cut(s) 790, 1124, 1142, 1227, 1788
Bse1I ACTGG 3 cut(s) 27, 120, 1745
Bse21I CCTNAGG 1 cut(s) 147
Bse3DI GCAATG 1 cut(s) 1057
BseBI CCWGG 2 cut(s) 197, 1077
BseDI CCNNGG 2 cut(s) 451, 1340
BseGI GGATG 5 cut(s) 381, 649, 685, 799, 1749
BseLI CCNNNNNNNGG 5 cut(s) 790, 1124, 1142, 1227, 1788
BseMI GCAATG 1 cut(s) 1057
BseMII CTCAG 3 cut(s) 138, 258, 978
BseNI ACTGG 3 cut(s) 27, 120, 1745
BseRI GAGGAG 5 cut(s) 284, 309, 476, 664, 701
BseSI GKGCMC 1 cut(s) 1795
BseXI GCAGC 4 cut(s) 799, 970, 1638, 1734
BseYI CCCAGC 2 cut(s) 379, 1637
BsgI GTGCAG 1 cut(s) 522
BshFI GGCC 3 cut(s) 1045, 1597, 1737
BshNI GGYRCC 1 cut(s) 1704
BslFI GGGAC 1 cut(s) 542
BslI CCNNNNNNNGG 5 cut(s) 790, 1124, 1142, 1227, 1788
BsmAI GTCTC 5 cut(s) 279, 705, 1148, 1295, 1313
BsmFI GGGAC 1 cut(s) 542
BsnI GGCC 3 cut(s) 1045, 1597, 1737
Bsp119I TTCGAA 1 cut(s) 1624
Bsp1286I GDGCHC 2 cut(s) 877, 1795
Bsp143I GATC 9 cut(s) 4, 37, 82, 94, 340, 751, 954, 1409, 1507
BspACI CCGC 3 cut(s) 668, 785, 1782
BspANI GGCC 3 cut(s) 1045, 1597, 1737
BspCNI CTCAG 3 cut(s) 139, 259, 979
BspHI TCATGA 3 cut(s) 280, 939, 1671
BspLI GGNNCC 4 cut(s) 6, 111, 1411, 1706
BspPI GGATC 4 cut(s) 12, 77, 1404, 1417
BspQI GCTCTTC 2 cut(s) 261, 882
BspT104I TTCGAA 1 cut(s) 1624
BspT107I GGYRCC 1 cut(s) 1704
BsrDI GCAATG 1 cut(s) 1057
BsrI ACTGG 3 cut(s) 27, 120, 1745
BssECI CCNNGG 2 cut(s) 451, 1340
BssMI GATC 9 cut(s) 4, 37, 82, 94, 340, 751, 954, 1409, 1507
BssSI CACGAG 1 cut(s) 1500
BssT1I CCWWGG 1 cut(s) 451
Bst2BI CACGAG 1 cut(s) 1500
Bst2UI CCWGG 2 cut(s) 197, 1077
Bst4CI ACNGT 5 cut(s) 176, 1324, 1337, 1351, 1664
Bst6I CTCTTC 4 cut(s) 133, 261, 282, 882
BstBI TTCGAA 1 cut(s) 1624
BstC8I GCNNGC 3 cut(s) 785, 1047, 1278
BstDEI CTNAG 6 cut(s) 98, 147, 267, 419, 987, 1253
BstDSI CCRYGG 1 cut(s) 1340
BstF5I GGATG 5 cut(s) 381, 649, 685, 799, 1749
BstKTI GATC 9 cut(s) 7, 40, 85, 97, 343, 754, 957, 1412, 1510
BstMAI GTCTC 5 cut(s) 279, 705, 1148, 1295, 1313
BstMBI GATC 9 cut(s) 4, 37, 82, 94, 340, 751, 954, 1409, 1507
BstMWI GCNNNNNNNGC 6 cut(s) 260, 730, 796, 923, 992, 1790
BstNI CCWGG 2 cut(s) 197, 1077
BstSCI CCNGG 2 cut(s) 195, 1075
BstSLI GKGCMC 1 cut(s) 1795
BstV1I GCAGC 4 cut(s) 799, 970, 1638, 1734
BstV2I GAAGAC 1 cut(s) 1078
BstX2I RGATCY 3 cut(s) 4, 94, 1409
BstXI CCANNNNNNTGG 1 cut(s) 572
BstYI RGATCY 3 cut(s) 4, 94, 1409
Bsu36I CCTNAGG 1 cut(s) 147
BsuI GTATCC 3 cut(s) 1034, 1339, 1480
BsuRI GGCC 3 cut(s) 1045, 1597, 1737
BtgI CCRYGG 1 cut(s) 1340
BtgZI GCGATG 1 cut(s) 1148
BtrI CACGTC 1 cut(s) 1547
BtsCI GGATG 5 cut(s) 381, 649, 685, 799, 1749
BtsI GCAGTG 1 cut(s) 1619
BtsIMutI CAGTG 4 cut(s) 181, 264, 1619, 1738
Cac8I GCNNGC 3 cut(s) 785, 1047, 1278
CciI TCATGA 3 cut(s) 280, 939, 1671
Cfr13I GGNCC 3 cut(s) 109, 1082, 1660
CsiI ACCWGGT 2 cut(s) 195, 1075
Csp6I GTAC 5 cut(s) 70, 187, 1207, 1306, 1705
CviAII CATG 7 cut(s) 242, 281, 940, 1418, 1630, 1672, 1751
CviQI GTAC 5 cut(s) 70, 187, 1207, 1306, 1705
DdeI CTNAG 6 cut(s) 98, 147, 267, 419, 987, 1253
DpnI GATC 9 cut(s) 6, 39, 84, 96, 342, 753, 956, 1411, 1509
DpnII GATC 9 cut(s) 4, 37, 82, 94, 340, 751, 954, 1409, 1507
DraI TTTAAA 1 cut(s) 978
DrdI GACNNNNNNGTC 2 cut(s) 755, 1667
DseDI GACNNNNNNGTC 2 cut(s) 755, 1667
EaeI YGGCCR 2 cut(s) 1043, 1735
Eam1104I CTCTTC 4 cut(s) 133, 261, 282, 882
EarI CTCTTC 4 cut(s) 133, 261, 282, 882
EciI GGCGGA 1 cut(s) 1771
Eco130I CCWWGG 1 cut(s) 451
Eco24I GRGCYC 1 cut(s) 877
Eco47I GGWCC 3 cut(s) 109, 1082, 1660
Eco57I CTGAAG 1 cut(s) 923
Eco81I CCTNAGG 1 cut(s) 147
EcoRI GAATTC 1 cut(s) 1767
EcoRII CCWGG 2 cut(s) 195, 1075
EcoT14I CCWWGG 1 cut(s) 451
EcoT38I GRGCYC 1 cut(s) 877
ErhI CCWWGG 1 cut(s) 451
FaeI CATG 7 cut(s) 245, 284, 943, 1421, 1633, 1675, 1754
FaqI GGGAC 1 cut(s) 542
FatI CATG 7 cut(s) 241, 280, 939, 1417, 1629, 1671, 1750
FauI CCCGC 1 cut(s) 792
FbaI TGATCA 2 cut(s) 37, 340
Fnu4HI GCNGC 4 cut(s) 788, 984, 1652, 1748
FokI GGATG 5 cut(s) 388, 656, 692, 806, 1756
FriOI GRGCYC 1 cut(s) 877
Fsp4HI GCNGC 4 cut(s) 788, 984, 1652, 1748
FspBI CTAG 4 cut(s) 452, 483, 600, 1800
GluI GCNGC 4 cut(s) 788, 984, 1652, 1748
GsaI CCCAGC 2 cut(s) 383, 1641
GsuI CTGGAG 1 cut(s) 564
HaeIII GGCC 3 cut(s) 1045, 1597, 1737
Hin1II CATG 7 cut(s) 245, 284, 943, 1421, 1633, 1675, 1754
HindIII AAGCTT 2 cut(s) 136, 915
HinfI GANTC 7 cut(s) 272, 838, 896, 1146, 1299, 1375, 1621
HphI GGTGA 2 cut(s) 695, 704
Hpy166II GTNNAC 3 cut(s) 1082, 1333, 1340
Hpy188I TCNGA 8 cut(s) 19, 172, 765, 882, 1089, 1445, 1512, 1646
Hpy8I GTNNAC 3 cut(s) 1082, 1333, 1340
HpyAV CCTTC 7 cut(s) 96, 817, 847, 1136, 1435, 1722, 1753
HpyCH4III ACNGT 5 cut(s) 176, 1324, 1337, 1351, 1664
HpyCH4IV ACGT 2 cut(s) 10, 1546
HpyCH4V TGCA 8 cut(s) 220, 442, 503, 926, 962, 1421, 1469, 1750
HpyF10VI GCNNNNNNNGC 6 cut(s) 260, 730, 796, 923, 992, 1790
HpyF3I CTNAG 6 cut(s) 98, 147, 267, 419, 987, 1253
HpySE526I ACGT 2 cut(s) 10, 1546
Hsp92II CATG 7 cut(s) 245, 284, 943, 1421, 1633, 1675, 1754
KpnI GGTACC 1 cut(s) 1708
Ksp22I TGATCA 2 cut(s) 37, 340
Kzo9I GATC 9 cut(s) 4, 37, 82, 94, 340, 751, 954, 1409, 1507
LguI GCTCTTC 2 cut(s) 261, 882
LmnI GCTCC 1 cut(s) 770
Lsp1109I GCAGC 4 cut(s) 799, 970, 1638, 1734
LweI GCATC 3 cut(s) 366, 1171, 1734
MabI ACCWGGT 2 cut(s) 195, 1075
MaeI CTAG 4 cut(s) 452, 483, 600, 1800
MaeII ACGT 2 cut(s) 10, 1546
MaeIII GTNAC 2 cut(s) 122, 653
MalI GATC 9 cut(s) 6, 39, 84, 96, 342, 753, 956, 1411, 1509
MboI GATC 9 cut(s) 4, 37, 82, 94, 340, 751, 954, 1409, 1507
MfeI CAATTG 1 cut(s) 491
MflI RGATCY 3 cut(s) 4, 94, 1409
MhlI GDGCHC 2 cut(s) 877, 1795
MlsI TGGCCA 2 cut(s) 1045, 1737
MluNI TGGCCA 2 cut(s) 1045, 1737
MlyI GAGTC 2 cut(s) 281, 1155
MmeI TCCRAC 2 cut(s) 787, 1464
Mox20I TGGCCA 2 cut(s) 1045, 1737
MscI TGGCCA 2 cut(s) 1045, 1737
MseI TTAA 7 cut(s) 207, 447, 977, 1191, 1284, 1361, 1404
MslI CAYNNNNRTG 1 cut(s) 588
Msp20I TGGCCA 2 cut(s) 1045, 1737
MspA1I CMGCKG 1 cut(s) 1651
MspR9I CCNGG 2 cut(s) 197, 1077
MunI CAATTG 1 cut(s) 491
MvaI CCWGG 2 cut(s) 197, 1077
MwoI GCNNNNNNNGC 6 cut(s) 260, 730, 796, 923, 992, 1790
NdeII GATC 9 cut(s) 4, 37, 82, 94, 340, 751, 954, 1409, 1507
NlaIII CATG 7 cut(s) 245, 284, 943, 1421, 1633, 1675, 1754
NlaIV GGNNCC 4 cut(s) 6, 111, 1411, 1706
NspV TTCGAA 1 cut(s) 1624
PagI TCATGA 3 cut(s) 280, 939, 1671
PciSI GCTCTTC 2 cut(s) 261, 882
PfeI GAWTC 5 cut(s) 838, 896, 1299, 1375, 1621
PflFI GACNNNGTC 1 cut(s) 176
PflMI CCANNNNNTGG 1 cut(s) 1227
PkrI GCNGC 4 cut(s) 789, 985, 1653, 1749
PleI GAGTC 2 cut(s) 280, 1154
PpsI GAGTC 2 cut(s) 280, 1154
Psp6I CCWGG 2 cut(s) 195, 1075
PspFI CCCAGC 2 cut(s) 379, 1637
PspGI CCWGG 2 cut(s) 195, 1075
PspN4I GGNNCC 4 cut(s) 6, 111, 1411, 1706
PspPI GGNCC 3 cut(s) 109, 1082, 1660
PsuI RGATCY 3 cut(s) 4, 94, 1409
PsyI GACNNNGTC 1 cut(s) 176
PvuII CAGCTG 1 cut(s) 1651
RsaI GTAC 5 cut(s) 71, 188, 1208, 1307, 1706
RsaNI GTAC 5 cut(s) 70, 187, 1207, 1306, 1705
RseI CAYNNNNRTG 1 cut(s) 588
SapI GCTCTTC 2 cut(s) 261, 882
SaqAI TTAA 7 cut(s) 207, 447, 977, 1191, 1284, 1361, 1404
SatI GCNGC 4 cut(s) 788, 984, 1652, 1748
Sau3AI GATC 9 cut(s) 4, 37, 82, 94, 340, 751, 954, 1409, 1507
Sau96I GGNCC 3 cut(s) 109, 1082, 1660
ScaI AGTACT 1 cut(s) 1208
SchI GAGTC 2 cut(s) 281, 1155
ScrFI CCNGG 2 cut(s) 197, 1077
SduI GDGCHC 2 cut(s) 877, 1795
SexAI ACCWGGT 2 cut(s) 195, 1075
SfaNI GCATC 3 cut(s) 366, 1171, 1734
SfuI TTCGAA 1 cut(s) 1624
SinI GGWCC 3 cut(s) 109, 1082, 1660
SmiMI CAYNNNNRTG 1 cut(s) 588
SmlI CTYRAG 4 cut(s) 90, 182, 1066, 1120
SmoI CTYRAG 4 cut(s) 90, 182, 1066, 1120
SsiI CCGC 3 cut(s) 668, 785, 1782
SspMI CTAG 4 cut(s) 452, 483, 600, 1800
StyD4I CCNGG 2 cut(s) 195, 1075
StyI CCWWGG 1 cut(s) 451
TaaI ACNGT 5 cut(s) 176, 1324, 1337, 1351, 1664
TaiI ACGT 2 cut(s) 13, 1549
TaqI TCGA 2 cut(s) 1132, 1624
TatI WGTACW 2 cut(s) 186, 1206
TfiI GAWTC 5 cut(s) 838, 896, 1299, 1375, 1621
Tru1I TTAA 7 cut(s) 207, 447, 977, 1191, 1284, 1361, 1404
Tru9I TTAA 7 cut(s) 207, 447, 977, 1191, 1284, 1361, 1404
TscAI CASTG 4 cut(s) 181, 271, 1619, 1745
TseI GCWGC 4 cut(s) 787, 983, 1651, 1747
TspDTI ATGAA 9 cut(s) 120, 269, 876, 928, 956, 1046, 1688, 1743, 1767
TspGWI ACGGA 1 cut(s) 1016
TspRI CASTG 4 cut(s) 181, 271, 1619, 1745
Tth111I GACNNNGTC 1 cut(s) 176
Van91I CCANNNNNTGG 1 cut(s) 1227
VpaK11BI GGWCC 3 cut(s) 109, 1082, 1660
XapI RAATTY 4 cut(s) 20, 1357, 1369, 1767
XbaI TCTAGA 1 cut(s) 599
XmaJI CCTAGG 1 cut(s) 451
XspI CTAG 4 cut(s) 452, 483, 600, 1800
ZrmI AGTACT 1 cut(s) 1208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.