Rh5DG457800

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000624
Physical Location & Seq
Forward (+)
72775668 .. 72776717
1050 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG457800.1

Sequence Viewer

Length: 1050 bp
ATGCAGCACTCTTCCCCATTTGGTGATTGTACTCAGAGGTTGGCTCATTGCTTTGCAAATGCTCTTGAAGCGCGGTTGGCTGGCACAGGAACTCAAATATATACTGCTTTAACTACTAAACGAATGTCAGCTGCTGATTCGCTGAAGTTTTACCAAGCTTATATTGCAGTCTGCCCGTTCATGAAGTTGTTGATGATCTTTGCAAACGATATGATTTTAAAAGCTGCTAAGAAAGCAGAAACACTTCATATAATAGATTTTGGTATCCTTTATGGTTTTCAATGGCCTGCTCTCATTCATTGCCTCTCAAGAAGACCTGGTGGACCTCCGAAATTACGCATTACAGGGGTAGAGCTTCCCTCTAGTGGTTTTCGACCAGAAGAAGGAGTCCAAGAGACAGGGCATCGCTTGGCAAAGTATTGTAGGCGATTTAATGTTCATTTTGAGTACTATGGCATAGCCAAAAAATGGGAAACTCTCCAATTTGATGACCTTAGAGTTCAAAGAAATGAGGTGCTTGCTGTTAATTGTCTCTGCCGATTTAAGTACCTCCTTGATGAGACAGTTGTGGCAAACAGTCCAAGAGATACTGTTCTAAATTTAATTAGGAAGTTGAATCCTGATGTCTTTGTCCAAAGTGTTGTTAATGGATCCCATCATGCACCCTTCTTTGTTCCACGCTTCCGACAGGCACTGTTCCACTTCTCTGCATTGTTTGATATGTTGGATAACAATTTGCCTCCTGGCGATCAGATGAGATTGGGGTTTGAGAAAGAGTTTATTGGACGTGAAGTTGTGAATATAGTAGCTTGTGAGGGCTCTCAAAGAATTGTTAGGCCAGAAACATTTAAGCAGTGGCAGATTCGAAACATGAGGGCTGGGTTCAGACAGCTACCATTGGACCGTGAAGTCATGAACAAAATAAAGGATAAGGTAAAAATGGGGTACCACCCTTATTTTGTTGTTGATGAAGATGGCCACTGGATGCTGCAGGGATGGAAGGGCAGAATTATGTTTGCTTCTGCCTGTTGGGCACCATCTAGGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

349

Amino Acids

40.18

Weight (kDa)

9.38

Isoelectric Point (pI)

43.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 2 - 344 1.5e-109 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 908
Acc65I GGTACC 1 cut(s) 945
AccB1I GGYRCC 2 cut(s) 945, 1033
AccB7I CCANNNNNTGG 1 cut(s) 468
AccII CGCG 1 cut(s) 73
AciI CCGC 1 cut(s) 73
AclWI GGATC 2 cut(s) 645, 658
AcoI YGGCCR 1 cut(s) 976
AcsI RAATTY 1 cut(s) 598
AcuI CTGAAG 1 cut(s) 164
AfaI GTAC 4 cut(s) 31, 449, 548, 947
AfiI CCNNNNNNNGG 3 cut(s) 365, 383, 468
AgsI TTSAA 4 cut(s) 68, 281, 503, 616
AjiI CACGTC 1 cut(s) 788
AjnI CCWGG 2 cut(s) 316, 742
AluBI AGCT 6 cut(s) 131, 158, 224, 355, 809, 892
AluI AGCT 6 cut(s) 131, 158, 224, 355, 809, 892
Alw26I GTCTC 3 cut(s) 389, 536, 554
AlwI GGATC 2 cut(s) 645, 658
AlwNI CAGNNNCTG 2 cut(s) 134, 694
AoxI GGCC 3 cut(s) 284, 836, 976
ApeKI GCWGC 4 cut(s) 4, 131, 224, 988
ApoI RAATTY 1 cut(s) 598
Asp700I GAANNNNTTC 1 cut(s) 243
Asp718I GGTACC 1 cut(s) 945
AspLEI GCGC 1 cut(s) 73
AspS9I GGNCC 2 cut(s) 323, 901
AsuHPI GGTGA 1 cut(s) 35
AsuII TTCGAA 1 cut(s) 865
AvaII GGWCC 2 cut(s) 323, 901
BaeGI GKGCMC 1 cut(s) 1036
BalI TGGCCA 1 cut(s) 978
BamHI GGATCC 1 cut(s) 650
BanI GGYRCC 2 cut(s) 945, 1033
BanII GRGCYC 1 cut(s) 821
BbsI GAAGAC 1 cut(s) 319
BbvI GCAGC 4 cut(s) 16, 118, 211, 975
BccI CCATC 4 cut(s) 663, 968, 990, 1045
BciT130I CCWGG 2 cut(s) 318, 744
BciVI GTATCC 1 cut(s) 275
BcoDI GTCTC 3 cut(s) 389, 536, 554
BfaI CTAG 2 cut(s) 363, 1041
BfmI CTRYAG 1 cut(s) 989
BfuI GTATCC 1 cut(s) 275
BglI GCCNNNNNGGC 1 cut(s) 1031
BisI GCNGC 4 cut(s) 5, 132, 225, 989
BlsI GCNGC 4 cut(s) 6, 133, 226, 990
BmcAI AGTACT 1 cut(s) 449
Bme1390I CCNGG 2 cut(s) 318, 744
Bme18I GGWCC 2 cut(s) 323, 901
BmgBI CACGTC 1 cut(s) 788
BmgT120I GGNCC 2 cut(s) 323, 901
BmiI GGNNCC 3 cut(s) 652, 947, 1035
BmrFI CCNGG 2 cut(s) 318, 744
BmsI GCATC 2 cut(s) 412, 975
BpiI GAAGAC 1 cut(s) 319
BplI GAGNNNNNCTC 4 cut(s) 28, 60, 344, 376
Bpu14I TTCGAA 1 cut(s) 865
BpuEI CTTGAG 1 cut(s) 292
Bsc4I CCNNNNNNNGG 3 cut(s) 365, 383, 468
Bse1I ACTGG 1 cut(s) 986
Bse3DI GCAATG 2 cut(s) 46, 298
BseBI CCWGG 2 cut(s) 318, 744
BseGI GGATG 2 cut(s) 990, 1001
BseLI CCNNNNNNNGG 3 cut(s) 365, 383, 468
BseMI GCAATG 2 cut(s) 46, 298
BseMII CTCAG 1 cut(s) 47
BseNI ACTGG 1 cut(s) 986
BseSI GKGCMC 1 cut(s) 1036
BseXI GCAGC 4 cut(s) 16, 118, 211, 975
BseYI CCCAGC 1 cut(s) 878
Bsh1236I CGCG 1 cut(s) 73
BshFI GGCC 3 cut(s) 286, 838, 978
BshNI GGYRCC 2 cut(s) 945, 1033
BslI CCNNNNNNNGG 3 cut(s) 365, 383, 468
BsmAI GTCTC 3 cut(s) 389, 536, 554
BsnI GGCC 3 cut(s) 286, 838, 978
Bsp119I TTCGAA 1 cut(s) 865
Bsp1286I GDGCHC 2 cut(s) 821, 1036
Bsp143I GATC 3 cut(s) 195, 650, 748
BspACI CCGC 1 cut(s) 73
BspANI GGCC 3 cut(s) 286, 838, 978
BspCNI CTCAG 1 cut(s) 46
BspFNI CGCG 1 cut(s) 73
BspHI TCATGA 2 cut(s) 180, 912
BspLI GGNNCC 3 cut(s) 652, 947, 1035
BspMAI CTGCAG 1 cut(s) 993
BspPI GGATC 2 cut(s) 645, 658
BspT104I TTCGAA 1 cut(s) 865
BspT107I GGYRCC 2 cut(s) 945, 1033
BsrDI GCAATG 2 cut(s) 46, 298
BsrI ACTGG 1 cut(s) 986
BssMI GATC 3 cut(s) 195, 650, 748
Bst2UI CCWGG 2 cut(s) 318, 744
Bst4CI ACNGT 5 cut(s) 565, 578, 592, 696, 905
Bst6I CTCTTC 1 cut(s) 16
BstBI TTCGAA 1 cut(s) 865
BstC8I GCNNGC 3 cut(s) 82, 288, 519
BstDEI CTNAG 3 cut(s) 33, 228, 494
BstF5I GGATG 2 cut(s) 990, 1001
BstFNI CGCG 1 cut(s) 73
BstHHI GCGC 1 cut(s) 73
BstKTI GATC 3 cut(s) 198, 653, 751
BstMAI GTCTC 3 cut(s) 389, 536, 554
BstMBI GATC 3 cut(s) 195, 650, 748
BstMWI GCNNNNNNNGC 5 cut(s) 68, 77, 164, 233, 1031
BstNI CCWGG 2 cut(s) 318, 744
BstSCI CCNGG 2 cut(s) 316, 742
BstSFI CTRYAG 1 cut(s) 989
BstSLI GKGCMC 1 cut(s) 1036
BstUI CGCG 1 cut(s) 73
BstV1I GCAGC 4 cut(s) 16, 118, 211, 975
BstV2I GAAGAC 1 cut(s) 319
BstX2I RGATCY 1 cut(s) 650
BstYI RGATCY 1 cut(s) 650
BsuI GTATCC 1 cut(s) 275
BsuRI GGCC 3 cut(s) 286, 838, 978
BtgZI GCGATG 1 cut(s) 389
BtrI CACGTC 1 cut(s) 788
BtsCI GGATG 2 cut(s) 990, 1001
BtsI GCAGTG 1 cut(s) 860
BtsIMutI CAGTG 3 cut(s) 692, 860, 979
Cac8I GCNNGC 3 cut(s) 82, 288, 519
CaiI CAGNNNCTG 2 cut(s) 134, 694
CciI TCATGA 2 cut(s) 180, 912
CfoI GCGC 1 cut(s) 73
Cfr13I GGNCC 2 cut(s) 323, 901
CsiI ACCWGGT 1 cut(s) 316
Csp6I GTAC 4 cut(s) 30, 448, 547, 946
CviAII CATG 4 cut(s) 181, 659, 871, 913
CviQI GTAC 4 cut(s) 30, 448, 547, 946
DdeI CTNAG 3 cut(s) 33, 228, 494
DpnI GATC 3 cut(s) 197, 652, 750
DpnII GATC 3 cut(s) 195, 650, 748
DraI TTTAAA 1 cut(s) 219
DrdI GACNNNNNNGTC 1 cut(s) 908
DseDI GACNNNNNNGTC 1 cut(s) 908
EaeI YGGCCR 1 cut(s) 976
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
Eco24I GRGCYC 1 cut(s) 821
Eco47I GGWCC 2 cut(s) 323, 901
Eco57I CTGAAG 1 cut(s) 164
EcoRII CCWGG 2 cut(s) 316, 742
EcoT38I GRGCYC 1 cut(s) 821
FaeI CATG 4 cut(s) 184, 662, 874, 916
FatI CATG 4 cut(s) 180, 658, 870, 912
Fnu4HI GCNGC 4 cut(s) 5, 132, 225, 989
FokI GGATG 2 cut(s) 997, 1008
FriOI GRGCYC 1 cut(s) 821
Fsp4HI GCNGC 4 cut(s) 5, 132, 225, 989
FspBI CTAG 2 cut(s) 363, 1041
GlaI GCGC 1 cut(s) 72
GluI GCNGC 4 cut(s) 5, 132, 225, 989
GsaI CCCAGC 1 cut(s) 882
HaeIII GGCC 3 cut(s) 286, 838, 978
HhaI GCGC 1 cut(s) 73
Hin1II CATG 4 cut(s) 184, 662, 874, 916
Hin6I GCGC 1 cut(s) 71
HinP1I GCGC 1 cut(s) 71
HindIII AAGCTT 1 cut(s) 156
HinfI GANTC 4 cut(s) 137, 387, 616, 862
HphI GGTGA 1 cut(s) 35
Hpy166II GTNNAC 1 cut(s) 323
Hpy188I TCNGA 5 cut(s) 36, 330, 686, 753, 887
Hpy188III TCNNGA 5 cut(s) 65, 181, 309, 620, 913
Hpy8I GTNNAC 1 cut(s) 323
HpyAV CCTTC 3 cut(s) 377, 676, 994
HpyCH4III ACNGT 5 cut(s) 565, 578, 592, 696, 905
HpyCH4IV ACGT 1 cut(s) 787
HpyCH4V TGCA 7 cut(s) 4, 56, 167, 203, 662, 710, 991
HpyF10VI GCNNNNNNNGC 5 cut(s) 68, 77, 164, 233, 1031
HpyF3I CTNAG 3 cut(s) 33, 228, 494
HpySE526I ACGT 1 cut(s) 787
Hsp92II CATG 4 cut(s) 184, 662, 874, 916
HspAI GCGC 1 cut(s) 71
KpnI GGTACC 1 cut(s) 949
Kzo9I GATC 3 cut(s) 195, 650, 748
Lsp1109I GCAGC 4 cut(s) 16, 118, 211, 975
LweI GCATC 2 cut(s) 412, 975
MabI ACCWGGT 1 cut(s) 316
MaeI CTAG 2 cut(s) 363, 1041
MaeII ACGT 1 cut(s) 787
MalI GATC 3 cut(s) 197, 652, 750
MboI GATC 3 cut(s) 195, 650, 748
MboII GAAGA 4 cut(s) 3, 324, 392, 983
MflI RGATCY 1 cut(s) 650
MhlI GDGCHC 2 cut(s) 821, 1036
MlsI TGGCCA 1 cut(s) 978
MluCI AATT 8 cut(s) 332, 482, 526, 598, 603, 733, 828, 1008
MluNI TGGCCA 1 cut(s) 978
MlyI GAGTC 1 cut(s) 396
MmeI TCCRAC 2 cut(s) 705, 709
MnlI CCTC 9 cut(s) 30, 314, 336, 370, 505, 560, 750, 808, 867
Mox20I TGGCCA 1 cut(s) 978
MroXI GAANNNNTTC 1 cut(s) 243
MscI TGGCCA 1 cut(s) 978
MseI TTAA 8 cut(s) 110, 218, 432, 525, 543, 602, 645, 849
Msp20I TGGCCA 1 cut(s) 978
MspA1I CMGCKG 1 cut(s) 131
MspR9I CCNGG 2 cut(s) 318, 744
MvaI CCWGG 2 cut(s) 318, 744
MvnI CGCG 1 cut(s) 73
MwoI GCNNNNNNNGC 5 cut(s) 68, 77, 164, 233, 1031
NdeII GATC 3 cut(s) 195, 650, 748
NlaIII CATG 4 cut(s) 184, 662, 874, 916
NlaIV GGNNCC 3 cut(s) 652, 947, 1035
NspV TTCGAA 1 cut(s) 865
PagI TCATGA 2 cut(s) 180, 912
PdmI GAANNNNTTC 1 cut(s) 243
PfeI GAWTC 3 cut(s) 137, 616, 862
PflMI CCANNNNNTGG 1 cut(s) 468
PkrI GCNGC 4 cut(s) 6, 133, 226, 990
PleI GAGTC 1 cut(s) 395
PpsI GAGTC 1 cut(s) 395
Psp6I CCWGG 2 cut(s) 316, 742
PspFI CCCAGC 1 cut(s) 878
PspGI CCWGG 2 cut(s) 316, 742
PspN4I GGNNCC 3 cut(s) 652, 947, 1035
PspPI GGNCC 2 cut(s) 323, 901
PstI CTGCAG 1 cut(s) 993
PstNI CAGNNNCTG 2 cut(s) 134, 694
PsuI RGATCY 1 cut(s) 650
PvuII CAGCTG 1 cut(s) 131
RsaI GTAC 4 cut(s) 31, 449, 548, 947
RsaNI GTAC 4 cut(s) 30, 448, 547, 946
SaqAI TTAA 8 cut(s) 110, 218, 432, 525, 543, 602, 645, 849
SatI GCNGC 4 cut(s) 5, 132, 225, 989
Sau3AI GATC 3 cut(s) 195, 650, 748
Sau96I GGNCC 2 cut(s) 323, 901
ScaI AGTACT 1 cut(s) 449
SchI GAGTC 1 cut(s) 396
ScrFI CCNGG 2 cut(s) 318, 744
SduI GDGCHC 2 cut(s) 821, 1036
SexAI ACCWGGT 1 cut(s) 316
SfaNI GCATC 2 cut(s) 412, 975
SfcI CTRYAG 1 cut(s) 989
SfuI TTCGAA 1 cut(s) 865
SinI GGWCC 2 cut(s) 323, 901
SmlI CTYRAG 1 cut(s) 307
SmoI CTYRAG 1 cut(s) 307
Sse9I AATT 8 cut(s) 332, 482, 526, 598, 603, 733, 828, 1008
SsiI CCGC 1 cut(s) 73
SspMI CTAG 2 cut(s) 363, 1041
StyD4I CCNGG 2 cut(s) 316, 742
TaaI ACNGT 5 cut(s) 565, 578, 592, 696, 905
TaiI ACGT 1 cut(s) 790
TaqI TCGA 2 cut(s) 373, 865
TasI AATT 8 cut(s) 332, 482, 526, 598, 603, 733, 828, 1008
TatI WGTACW 2 cut(s) 29, 447
TfiI GAWTC 3 cut(s) 137, 616, 862
Tru1I TTAA 8 cut(s) 110, 218, 432, 525, 543, 602, 645, 849
Tru9I TTAA 8 cut(s) 110, 218, 432, 525, 543, 602, 645, 849
TscAI CASTG 3 cut(s) 699, 860, 986
TseI GCWGC 4 cut(s) 4, 131, 224, 988
TspDTI ATGAA 7 cut(s) 169, 197, 236, 287, 428, 929, 984
TspRI CASTG 3 cut(s) 699, 860, 986
Van91I CCANNNNNTGG 1 cut(s) 468
VpaK11BI GGWCC 2 cut(s) 323, 901
XapI RAATTY 1 cut(s) 598
XmnI GAANNNNTTC 1 cut(s) 243
XspI CTAG 2 cut(s) 363, 1041
ZrmI AGTACT 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.