Belongs to the GRAS family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 2 cut(s) | 80, 1181 |
| Acc65I | GGTACC | 1 cut(s) | 1218 |
| AccB1I | GGYRCC | 2 cut(s) | 1218, 1306 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 741 |
| AccII | CGCG | 1 cut(s) | 243 |
| AciI | CCGC | 1 cut(s) | 156 |
| AclWI | GGATC | 2 cut(s) | 918, 931 |
| AcoI | YGGCCR | 2 cut(s) | 557, 1249 |
| AcsI | RAATTY | 1 cut(s) | 1281 |
| AcuI | CTGAAG | 1 cut(s) | 437 |
| AfaI | GTAC | 2 cut(s) | 722, 1220 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 115, 638, 656, 741 |
| AgsI | TTSAA | 6 cut(s) | 341, 454, 554, 776, 889, 1097 |
| AjiI | CACGTC | 1 cut(s) | 1061 |
| AjnI | CCWGG | 1 cut(s) | 589 |
| Alw21I | GWGCWC | 2 cut(s) | 220, 307 |
| Alw26I | GTCTC | 2 cut(s) | 239, 662 |
| Alw44I | GTGCAC | 1 cut(s) | 216 |
| AlwI | GGATC | 2 cut(s) | 918, 931 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 407 |
| AoxI | GGCC | 3 cut(s) | 557, 1109, 1249 |
| ApaLI | GTGCAC | 1 cut(s) | 216 |
| ApeKI | GCWGC | 6 cut(s) | 112, 277, 404, 497, 1165, 1261 |
| ApoI | RAATTY | 1 cut(s) | 1281 |
| AseI | ATTAAT | 1 cut(s) | 875 |
| Asp718I | GGTACC | 1 cut(s) | 1218 |
| AspS9I | GGNCC | 2 cut(s) | 596, 1174 |
| AsuHPI | GGTGA | 3 cut(s) | 20, 29, 308 |
| AsuII | TTCGAA | 1 cut(s) | 1138 |
| AvaII | GGWCC | 2 cut(s) | 596, 1174 |
| BaeGI | GKGCMC | 2 cut(s) | 220, 1309 |
| BalI | TGGCCA | 2 cut(s) | 559, 1251 |
| BamHI | GGATCC | 1 cut(s) | 923 |
| BanI | GGYRCC | 2 cut(s) | 1218, 1306 |
| BanII | GRGCYC | 1 cut(s) | 202 |
| BauI | CACGAG | 1 cut(s) | 1014 |
| BbsI | GAAGAC | 1 cut(s) | 592 |
| Bbv12I | GWGCWC | 2 cut(s) | 220, 307 |
| BbvI | GCAGC | 6 cut(s) | 124, 289, 391, 484, 1152, 1248 |
| BccI | CCATC | 4 cut(s) | 113, 936, 1241, 1263 |
| BciT130I | CCWGG | 1 cut(s) | 591 |
| BciVI | GTATCC | 4 cut(s) | 548, 739, 853, 994 |
| BcoDI | GTCTC | 2 cut(s) | 239, 662 |
| BfaI | CTAG | 2 cut(s) | 252, 1314 |
| BfmI | CTRYAG | 2 cut(s) | 110, 1262 |
| BfuI | GTATCC | 4 cut(s) | 548, 739, 853, 994 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 121, 1304 |
| BisI | GCNGC | 7 cut(s) | 113, 157, 278, 405, 498, 1166, 1262 |
| BlsI | GCNGC | 7 cut(s) | 114, 158, 279, 406, 499, 1167, 1263 |
| BmcAI | AGTACT | 1 cut(s) | 722 |
| Bme1390I | CCNGG | 1 cut(s) | 591 |
| Bme18I | GGWCC | 2 cut(s) | 596, 1174 |
| BmgBI | CACGTC | 1 cut(s) | 1061 |
| BmgT120I | GGNCC | 2 cut(s) | 596, 1174 |
| BmiI | GGNNCC | 3 cut(s) | 925, 1220, 1308 |
| BmrFI | CCNGG | 1 cut(s) | 591 |
| BmsI | GCATC | 2 cut(s) | 685, 1248 |
| BpiI | GAAGAC | 1 cut(s) | 592 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 301, 333, 617, 649 |
| Bpu14I | TTCGAA | 1 cut(s) | 1138 |
| BpuEI | CTTGAG | 2 cut(s) | 565, 619 |
| BsaJI | CCNNGG | 1 cut(s) | 854 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 115, 638, 656, 741 |
| Bse118I | RCCGGY | 1 cut(s) | 810 |
| Bse1I | ACTGG | 2 cut(s) | 364, 1259 |
| Bse3DI | GCAATG | 2 cut(s) | 319, 571 |
| BseBI | CCWGG | 1 cut(s) | 591 |
| BseDI | CCNNGG | 1 cut(s) | 854 |
| BseGI | GGATG | 4 cut(s) | 10, 124, 1263, 1274 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 115, 638, 656, 741 |
| BseMI | GCAATG | 2 cut(s) | 319, 571 |
| BseMII | CTCAG | 2 cut(s) | 320, 492 |
| BseNI | ACTGG | 2 cut(s) | 364, 1259 |
| BseRI | GAGGAG | 1 cut(s) | 26 |
| BseSI | GKGCMC | 2 cut(s) | 220, 1309 |
| BseXI | GCAGC | 6 cut(s) | 124, 289, 391, 484, 1152, 1248 |
| BseYI | CCCAGC | 1 cut(s) | 1151 |
| Bsh1236I | CGCG | 1 cut(s) | 243 |
| BshFI | GGCC | 3 cut(s) | 559, 1111, 1251 |
| BshNI | GGYRCC | 2 cut(s) | 1218, 1306 |
| BsiHKAI | GWGCWC | 2 cut(s) | 220, 307 |
| BsiSI | CCGG | 2 cut(s) | 116, 811 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 115, 638, 656, 741 |
| BsmAI | GTCTC | 2 cut(s) | 239, 662 |
| BsnI | GGCC | 3 cut(s) | 559, 1111, 1251 |
| Bsp119I | TTCGAA | 1 cut(s) | 1138 |
| Bsp1286I | GDGCHC | 4 cut(s) | 202, 220, 307, 1309 |
| Bsp143I | GATC | 5 cut(s) | 76, 238, 468, 923, 1021 |
| Bsp68I | TCGCGA | 1 cut(s) | 243 |
| BspACI | CCGC | 1 cut(s) | 156 |
| BspANI | GGCC | 3 cut(s) | 559, 1111, 1251 |
| BspCNI | CTCAG | 2 cut(s) | 319, 493 |
| BspFNI | CGCG | 1 cut(s) | 243 |
| BspHI | TCATGA | 1 cut(s) | 1185 |
| BspLI | GGNNCC | 3 cut(s) | 925, 1220, 1308 |
| BspMAI | CTGCAG | 2 cut(s) | 114, 1266 |
| BspPI | GGATC | 2 cut(s) | 918, 931 |
| BspQI | GCTCTTC | 1 cut(s) | 207 |
| BspT104I | TTCGAA | 1 cut(s) | 1138 |
| BspT107I | GGYRCC | 2 cut(s) | 1218, 1306 |
| BsrDI | GCAATG | 2 cut(s) | 319, 571 |
| BsrFI | RCCGGY | 1 cut(s) | 810 |
| BsrI | ACTGG | 2 cut(s) | 364, 1259 |
| BssAI | RCCGGY | 1 cut(s) | 810 |
| BssECI | CCNNGG | 1 cut(s) | 854 |
| BssMI | GATC | 5 cut(s) | 76, 238, 468, 923, 1021 |
| BssSI | CACGAG | 1 cut(s) | 1014 |
| BssT1I | CCWWGG | 1 cut(s) | 854 |
| Bst2BI | CACGAG | 1 cut(s) | 1014 |
| Bst2UI | CCWGG | 1 cut(s) | 591 |
| Bst4CI | ACNGT | 4 cut(s) | 751, 851, 865, 1178 |
| Bst6I | CTCTTC | 2 cut(s) | 207, 289 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 274 |
| BstBI | TTCGAA | 1 cut(s) | 1138 |
| BstC8I | GCNNGC | 5 cut(s) | 110, 355, 445, 561, 792 |
| BstDEI | CTNAG | 2 cut(s) | 306, 501 |
| BstF5I | GGATG | 4 cut(s) | 10, 124, 1263, 1274 |
| BstFNI | CGCG | 1 cut(s) | 243 |
| BstKTI | GATC | 5 cut(s) | 79, 241, 471, 926, 1024 |
| BstMAI | GTCTC | 2 cut(s) | 239, 662 |
| BstMBI | GATC | 5 cut(s) | 76, 238, 468, 923, 1021 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 55, 121, 265, 274, 341, 350, 506, 1304 |
| BstNI | CCWGG | 1 cut(s) | 591 |
| BstSCI | CCNGG | 1 cut(s) | 589 |
| BstSFI | CTRYAG | 2 cut(s) | 110, 1262 |
| BstSLI | GKGCMC | 2 cut(s) | 220, 1309 |
| BstUI | CGCG | 1 cut(s) | 243 |
| BstV1I | GCAGC | 6 cut(s) | 124, 289, 391, 484, 1152, 1248 |
| BstV2I | GAAGAC | 1 cut(s) | 592 |
| BstX2I | RGATCY | 1 cut(s) | 923 |
| BstYI | RGATCY | 1 cut(s) | 923 |
| BsuI | GTATCC | 4 cut(s) | 548, 739, 853, 994 |
| BsuRI | GGCC | 3 cut(s) | 559, 1111, 1251 |
| BtgZI | GCGATG | 1 cut(s) | 662 |
| BtrI | CACGTC | 1 cut(s) | 1061 |
| BtsCI | GGATG | 4 cut(s) | 10, 124, 1263, 1274 |
| BtsI | GCAGTG | 1 cut(s) | 1133 |
| BtsIMutI | CAGTG | 3 cut(s) | 357, 1133, 1252 |
| BtuMI | TCGCGA | 1 cut(s) | 243 |
| Cac8I | GCNNGC | 5 cut(s) | 110, 355, 445, 561, 792 |
| CaiI | CAGNNNCTG | 1 cut(s) | 407 |
| CciI | TCATGA | 1 cut(s) | 1185 |
| Cfr10I | RCCGGY | 1 cut(s) | 810 |
| Cfr13I | GGNCC | 2 cut(s) | 596, 1174 |
| CsiI | ACCWGGT | 1 cut(s) | 589 |
| Csp6I | GTAC | 2 cut(s) | 721, 1219 |
| CviAII | CATG | 3 cut(s) | 932, 1144, 1186 |
| CviQI | GTAC | 2 cut(s) | 721, 1219 |
| DdeI | CTNAG | 2 cut(s) | 306, 501 |
| DpnI | GATC | 5 cut(s) | 78, 240, 470, 925, 1023 |
| DpnII | GATC | 5 cut(s) | 76, 238, 468, 923, 1021 |
| DraI | TTTAAA | 1 cut(s) | 492 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 80, 1181 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 80, 1181 |
| EaeI | YGGCCR | 2 cut(s) | 557, 1249 |
| Eam1104I | CTCTTC | 2 cut(s) | 207, 289 |
| EarI | CTCTTC | 2 cut(s) | 207, 289 |
| Eco130I | CCWWGG | 1 cut(s) | 854 |
| Eco24I | GRGCYC | 1 cut(s) | 202 |
| Eco47I | GGWCC | 2 cut(s) | 596, 1174 |
| Eco57I | CTGAAG | 1 cut(s) | 437 |
| EcoRI | GAATTC | 1 cut(s) | 1281 |
| EcoRII | CCWGG | 1 cut(s) | 589 |
| EcoT14I | CCWWGG | 1 cut(s) | 854 |
| EcoT38I | GRGCYC | 1 cut(s) | 202 |
| ErhI | CCWWGG | 1 cut(s) | 854 |
| FaeI | CATG | 3 cut(s) | 935, 1147, 1189 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 449, 481 |
| FatI | CATG | 3 cut(s) | 931, 1143, 1185 |
| Fnu4HI | GCNGC | 7 cut(s) | 113, 157, 278, 405, 498, 1166, 1262 |
| FokI | GGATG | 4 cut(s) | 17, 131, 1270, 1281 |
| FriOI | GRGCYC | 1 cut(s) | 202 |
| Fsp4HI | GCNGC | 7 cut(s) | 113, 157, 278, 405, 498, 1166, 1262 |
| FspBI | CTAG | 2 cut(s) | 252, 1314 |
| GluI | GCNGC | 7 cut(s) | 113, 157, 278, 405, 498, 1166, 1262 |
| GsaI | CCCAGC | 1 cut(s) | 1155 |
| HaeIII | GGCC | 3 cut(s) | 559, 1111, 1251 |
| HapII | CCGG | 2 cut(s) | 116, 811 |
| Hin1II | CATG | 3 cut(s) | 935, 1147, 1189 |
| HindIII | AAGCTT | 1 cut(s) | 429 |
| HinfI | GANTC | 5 cut(s) | 163, 660, 833, 889, 1135 |
| HpaII | CCGG | 2 cut(s) | 116, 811 |
| HphI | GGTGA | 3 cut(s) | 20, 29, 308 |
| Hpy166II | GTNNAC | 3 cut(s) | 218, 596, 847 |
| Hpy188I | TCNGA | 7 cut(s) | 28, 90, 207, 309, 959, 1026, 1160 |
| Hpy188III | TCNNGA | 8 cut(s) | 64, 242, 338, 582, 893, 907, 1016, 1186 |
| Hpy8I | GTNNAC | 3 cut(s) | 218, 596, 847 |
| HpyAV | CCTTC | 7 cut(s) | 142, 172, 650, 949, 1236, 1267, 1320 |
| HpyCH4III | ACNGT | 4 cut(s) | 751, 851, 865, 1178 |
| HpyCH4IV | ACGT | 1 cut(s) | 1060 |
| HpyCH4V | TGCA | 8 cut(s) | 112, 218, 277, 329, 476, 935, 983, 1264 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 55, 121, 265, 274, 341, 350, 506, 1304 |
| HpyF3I | CTNAG | 2 cut(s) | 306, 501 |
| HpySE526I | ACGT | 1 cut(s) | 1060 |
| Hsp92II | CATG | 3 cut(s) | 935, 1147, 1189 |
| KpnI | GGTACC | 1 cut(s) | 1222 |
| Kzo9I | GATC | 5 cut(s) | 76, 238, 468, 923, 1021 |
| LguI | GCTCTTC | 1 cut(s) | 207 |
| LmnI | GCTCC | 1 cut(s) | 95 |
| Lsp1109I | GCAGC | 6 cut(s) | 124, 289, 391, 484, 1152, 1248 |
| LweI | GCATC | 2 cut(s) | 685, 1248 |
| MabI | ACCWGGT | 1 cut(s) | 589 |
| MaeI | CTAG | 2 cut(s) | 252, 1314 |
| MaeII | ACGT | 1 cut(s) | 1060 |
| MalI | GATC | 5 cut(s) | 78, 240, 470, 925, 1023 |
| MboI | GATC | 5 cut(s) | 76, 238, 468, 923, 1021 |
| MboII | GAAGA | 6 cut(s) | 194, 276, 597, 665, 1031, 1256 |
| MflI | RGATCY | 1 cut(s) | 923 |
| MhlI | GDGCHC | 4 cut(s) | 202, 220, 307, 1309 |
| MlsI | TGGCCA | 2 cut(s) | 559, 1251 |
| MluCI | AATT | 9 cut(s) | 135, 605, 755, 799, 872, 876, 1006, 1101, 1281 |
| MluNI | TGGCCA | 2 cut(s) | 559, 1251 |
| MlyI | GAGTC | 2 cut(s) | 669, 842 |
| MmeI | TCCRAC | 2 cut(s) | 112, 978 |
| Mox20I | TGGCCA | 2 cut(s) | 559, 1251 |
| MscI | TGGCCA | 2 cut(s) | 559, 1251 |
| MseI | TTAA | 6 cut(s) | 491, 705, 798, 816, 875, 918 |
| Msp20I | TGGCCA | 2 cut(s) | 559, 1251 |
| MspA1I | CMGCKG | 3 cut(s) | 404, 839, 1165 |
| MspI | CCGG | 2 cut(s) | 116, 811 |
| MspR9I | CCNGG | 1 cut(s) | 591 |
| MvaI | CCWGG | 1 cut(s) | 591 |
| MvnI | CGCG | 1 cut(s) | 243 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 55, 121, 265, 274, 341, 350, 506, 1304 |
| NdeII | GATC | 5 cut(s) | 76, 238, 468, 923, 1021 |
| NlaIII | CATG | 3 cut(s) | 935, 1147, 1189 |
| NlaIV | GGNNCC | 3 cut(s) | 925, 1220, 1308 |
| NruI | TCGCGA | 1 cut(s) | 243 |
| NspV | TTCGAA | 1 cut(s) | 1138 |
| PagI | TCATGA | 1 cut(s) | 1185 |
| PciSI | GCTCTTC | 1 cut(s) | 207 |
| PfeI | GAWTC | 3 cut(s) | 163, 889, 1135 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 741 |
| PkrI | GCNGC | 7 cut(s) | 114, 158, 279, 406, 499, 1167, 1263 |
| PleI | GAGTC | 2 cut(s) | 668, 841 |
| PpsI | GAGTC | 2 cut(s) | 668, 841 |
| PshBI | ATTAAT | 1 cut(s) | 875 |
| Psp6I | CCWGG | 1 cut(s) | 589 |
| PspFI | CCCAGC | 1 cut(s) | 1151 |
| PspGI | CCWGG | 1 cut(s) | 589 |
| PspN4I | GGNNCC | 3 cut(s) | 925, 1220, 1308 |
| PspPI | GGNCC | 2 cut(s) | 596, 1174 |
| PstI | CTGCAG | 2 cut(s) | 114, 1266 |
| PstNI | CAGNNNCTG | 1 cut(s) | 407 |
| PsuI | RGATCY | 1 cut(s) | 923 |
| PvuII | CAGCTG | 3 cut(s) | 404, 839, 1165 |
| RruI | TCGCGA | 1 cut(s) | 243 |
| RsaI | GTAC | 2 cut(s) | 722, 1220 |
| RsaNI | GTAC | 2 cut(s) | 721, 1219 |
| SapI | GCTCTTC | 1 cut(s) | 207 |
| SaqAI | TTAA | 6 cut(s) | 491, 705, 798, 816, 875, 918 |
| SatI | GCNGC | 7 cut(s) | 113, 157, 278, 405, 498, 1166, 1262 |
| Sau3AI | GATC | 5 cut(s) | 76, 238, 468, 923, 1021 |
| Sau96I | GGNCC | 2 cut(s) | 596, 1174 |
| ScaI | AGTACT | 1 cut(s) | 722 |
| SchI | GAGTC | 2 cut(s) | 669, 842 |
| ScrFI | CCNGG | 1 cut(s) | 591 |
| SduI | GDGCHC | 4 cut(s) | 202, 220, 307, 1309 |
| SexAI | ACCWGGT | 1 cut(s) | 589 |
| SfaNI | GCATC | 2 cut(s) | 685, 1248 |
| SfcI | CTRYAG | 2 cut(s) | 110, 1262 |
| SfuI | TTCGAA | 1 cut(s) | 1138 |
| SinI | GGWCC | 2 cut(s) | 596, 1174 |
| SmlI | CTYRAG | 2 cut(s) | 580, 634 |
| SmoI | CTYRAG | 2 cut(s) | 580, 634 |
| Sse9I | AATT | 9 cut(s) | 135, 605, 755, 799, 872, 876, 1006, 1101, 1281 |
| SsiI | CCGC | 1 cut(s) | 156 |
| SspMI | CTAG | 2 cut(s) | 252, 1314 |
| StyD4I | CCNGG | 1 cut(s) | 589 |
| StyI | CCWWGG | 1 cut(s) | 854 |
| TaaI | ACNGT | 4 cut(s) | 751, 851, 865, 1178 |
| TaiI | ACGT | 1 cut(s) | 1063 |
| TaqI | TCGA | 2 cut(s) | 646, 1138 |
| TasI | AATT | 9 cut(s) | 135, 605, 755, 799, 872, 876, 1006, 1101, 1281 |
| TatI | WGTACW | 1 cut(s) | 720 |
| TauI | GCSGC | 1 cut(s) | 159 |
| TfiI | GAWTC | 3 cut(s) | 163, 889, 1135 |
| Tru1I | TTAA | 6 cut(s) | 491, 705, 798, 816, 875, 918 |
| Tru9I | TTAA | 6 cut(s) | 491, 705, 798, 816, 875, 918 |
| TscAI | CASTG | 3 cut(s) | 364, 1133, 1259 |
| TseI | GCWGC | 6 cut(s) | 112, 277, 404, 497, 1165, 1261 |
| TspDTI | ATGAA | 3 cut(s) | 560, 1202, 1257 |
| TspGWI | ACGGA | 1 cut(s) | 530 |
| TspRI | CASTG | 3 cut(s) | 364, 1133, 1259 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 741 |
| VneI | GTGCAC | 1 cut(s) | 216 |
| VpaK11BI | GGWCC | 2 cut(s) | 596, 1174 |
| VspI | ATTAAT | 1 cut(s) | 875 |
| XapI | RAATTY | 1 cut(s) | 1281 |
| XspI | CTAG | 2 cut(s) | 252, 1314 |
| ZrmI | AGTACT | 1 cut(s) | 722 |